Embed Size (px)
Citation preview
ppm (t1)0.05.010.0
0
50000
8.42
28.
416
8.37
78.
354
8.29
18.
272
8.22
18.
198
7.83
57.
829
7.81
37.
806
7.58
17.
571
7.56
07.
088
7.08
17.
066
7.05
8
4.07
33.
819
3.32
33.
169
2.96
32.
511
2.50
62.
502
2.49
72.
493
0.00
000
3.011.00
1.010.981.901.14
1.00
3.09
5.981.78
0.51
MedKoo Biosciences www.medkoo.com [email protected]
MedKoo BiosciencesProduct Quality Control Datawww.medkoo.com [email protected]
MedKoo BiosciencesProduct Quality Control Datawww.medkoo.com [email protected]
MedKoo BiosciencesProduct Quality Control Datawww.medkoo.com [email protected]
min
mV
0 5 10 15 20 25 300
1000
Det.A Ch1
min
mV
0 5 10 15 20 25 300
1000
2000 Det.A Ch2
Det.A Ch1/220nm1Det.A Ch2/254nm2
: Diamonsil C18(2)，5um 4.6*250mm: Solvent A: H2O(0.05%H3PO4) Solvent B:ACN(0.05%H3PO4) : 35 ℃ : 0.8mL/min : 6min gradient(5%B), 9min gradient (5%-95%B), 5min gradient (95%B),then 5min @(5%B): UV : 220/280 nm : 1369773-39-6
Column
Mobile phase Temperature Flow rate Run time Detector Wavelenth Sample name
PeakTableDetector A Ch1 220nmPeak#
1 2 3 4
Total
Ret. Time 7.878 9.792 10.409
10.805
Area 700
5050 34907
7829924 7870581
Area % 0.009 0.064 0.444
99.483 100.000
Theoretical Plate# 54041.700 62964.880 76593.122
87211.688
Data File NameMethod File Name
: 1369773-39-6.lcd : 1369773-39-6.lcm
Med
Koo
Bio
scie
nces
ww
w.m
edko
o.co
msa
les@
med
koo.
com
PeakTableDetector A Ch2 254nmPeak#
1 2 3 4
Total
Ret. Time 9.136 9.794 10.411 10.805
Area 554
2776 23040
10050042 10076412
Area % 0.006 0.028 0.229
99.738 100.000
Theoretical Plate# 86904.313 54097.807 64395.531
86371.242
MedKoo BiosciencesProduct Quality Control Datawww.medkoo.com [email protected]
MedKoo BiosciencesProduct Quality Control Datawww.medkoo.com [email protected]
MedKoo BiosciencesProduct Quality Control Datawww.medkoo.com [email protected]
LC-MS condition Column : Chromolith SpeedROD ,RP-18e,50*4.6mm LCMS-002Mobile Phase : A: H2O(0.05%FA)
B: CH3CNTemperature : 35℃ Flow rate : 2.5mL/min Run time : 0.1min@20%B,1.7min gradient(20-95% B), then0.7min@95% B,then0.4min@20% BInjection volume : 5 uLDetector : UV 220/254nm
: 100-1000: Postive/Negative: 1369773-39-6: 1369773-39-6.lcd
Mass range ScanUser Name Data Filename
<MS Chromatogram>
min
mAU
0.00 0.25 0.50 0.75 1.00 1.25 1.50 1.75 2.00 2.25 2.50 2.75
0
PDA Multi 1 220nm,4nm
1.3
96
min
mAU
0.00 0.25 0.50 0.75 1.00 1.25 1.50 1.75 2.00 2.25 2.50 2.75
0
500
PDA Multi 2 254nm,4nm 1
.398
MS Chromatogram
min
5,783,798 (-0.017min Delay)
(x1,000,000)5.03
0.25 0.50 0.75 1.00 1.25 1.50 1.75 2.00 2.25 2.50 2.75
TIC(+)@1TIC(-)@2
1 /
1.43
2
MS Spectrum
Med
Koo
Bio
scie
nces
ww
w.m
edko
o.co
msa
les@
med
koo.
com
Peak#:1 R.Time:1.432(Scan#:107)MassPeaks:463Spectrum Mode:Averaged 1.383-1.433(105-109)BG Mode:Calc Segment 1 - Event 1
m/z
1000000
100 200 300 400 500 600 700 800 900
121.
9
153.
7
203.
3
266.
3
307.
4
366.
3
412.
4
434.
4
485.
6
513.
6
572.
7
630.
6
675.
3
705.
6
748.
1
782.
1 845.
6
877.
6
928.
7
992.
7
MedKoo BiosciencesProduct Quality Control Datawww.medkoo.com [email protected]
MedKoo BiosciencesProduct Quality Control Datawww.medkoo.com [email protected]
MedKoo BiosciencesProduct Quality Control Datawww.medkoo.com [email protected]
