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Quasi-VivoQuasi-VivoTMTM Inter-connected Cell Culture Inter-connected Cell Culture
Dr Kate DarleyKirkstall Ltd.
Presentation April 2010
Quasi-VivoQuasi-VivoTMTM Cell Culture Systems Cell Culture Systems
Introduction to the Quasi-VivoTM system
System data
Future directions
About Kirkstall
Access to the Quasi-VivoTM system
Quasi-VivoQuasi-VivoTMTM Cell Culture Systems Cell Culture Systems
Launch of company, November 2006
South Yorkshire Seedcorn Fund investment July 2007
Licence with Pisa University and Collaboration begins with Sheffield University, October 2007
Proof of Concept Testing complete, June 2008
Second Round Seed Funding, September 2008
Manufacture and first validation studies complete, November 2008
First Product (kit) sales, February 2009
Launch of Contract Research Service, April 2009
About Kirkstall
Quasi-VivoQuasi-VivoTMTM Cell Culture Systems Cell Culture Systems
Introduction to the Quasi-VivoTM system
Quasi-VivoQuasi-VivoTMTM Cell Culture Systems Cell Culture Systems
INTER-CONNECTED CELL CULTURE …
Cells/tissue connected in series/parallel
Nutrient/media flow-through system
Allows cell-to-cell signalling
Metabolites/drugs/compounds circulate in system
Vitality/cell differentiation maintained
PROVIDES IN-VIVO LIKE (Quasi-vivo) CONDITIONS FOR CELL GROWTH
Quasi-VivoQuasi-VivoTMTM Cell Culture Systems Cell Culture Systems
INTER-CONNECTED CELL CULTURE …
Quasi-VivoQuasi-VivoTMTM Cell Culture Systems Cell Culture Systems
Chamber designMinimises bubble formation and shear stressShape & dimensions similar to 24 well plates. Facilitates cell transfer …from seeding to the bio-module Cell biologists familiar with conventional multi-well plates rapidly able to use ...the system
Proprietary design/covered by three patents
Quasi-VivoQuasi-VivoTMTM Cell Culture Systems Cell Culture Systems
Experimental design
Basic - 2 chambers connectedIn series/parallelComplex – 32 chambers connected in series/parallel
Quasi-VivoQuasi-VivoTMTM Cell Culture Systems Cell Culture Systems
PROVIDES IN-VIVO LIKE CONDITIONS FOR CELL GROWTH?
VALIDATION IIn primary hepatocytes xenobiotic gene expression is closer to in vivo.Cells maintain differentiation over a longer periods
Cell morphologystatic Quasi-vivo
Quasi-VivoQuasi-VivoTMTM Cell Culture Systems Cell Culture Systems
VALIDATION II - Correlation with drug metabolism/toxicity in vivo?
Improved Sensitivity for
Toxicity Screening:
Diclofenac A non-steroidal anti-inflammatory.
Dose-response curve inHuman Primary hepatocytes
Quasi-VivoTM
EC50
Conventional In vitro EC50
Clinical Trial Result
Quasi-VivoQuasi-VivoTMTM Cell Culture Systems Cell Culture Systems
Access to the Quasi-VivoTM system
Quasi-VivoQuasi-VivoTMTM Cell Culture Systems Cell Culture Systems
Products: The Quasi-Vivo™ 100 Kit ACCESS TO THE TECHNOLOGY
Bio-modules, reservoir bottle, connecting tubes,Peristaltic pump (Kit).Services: Contract research
Flexible service, labs service available in Sheffield or in your own lab
Quasi-VivoQuasi-VivoTMTM Cell Culture Systems Cell Culture Systems
WHO WE WORK WITH….Academic groups: UK universities,France (Montpellier), Italy (Pisa), EU framework 7 – InLIVEtox
Cosmetic industry – EU ban 2009Need for an alternative to animal testing
Chemical industry – REACH legislationRegistration, Evaluation, Authorisation and restriction of CHemicals
Pharmaceutical industry- Lead discovery/lead optimization
KEY NOTE SPEAKERS:Prof David Tweats (University of Swansea) ‘Toxicity Testing in the 21st Century’Prof Claus-Michael Lehr (Helmholtz-Institute, Saarland) ‘Drug delivery across biological barriers’ Dr Patrick Maurel (INSERM Montpellier)‘Human hepatocyte culture and applications for drug discovery’Dr David Standring (Idenix Pharma, MA, USA) ‘Preclinical drug discovery, in vitro/in vivo testing past and future’Dr Robert Landsiedel (BASF) ‘Safety Research on Nanomaterials’Prof Sheila Macneil (University of Sheffield) ‘3D Tissue engineering models for R&D’
Quasi-VivoQuasi-VivoTMTM Cell Culture Systems Cell Culture Systems
Thanks for listening & any questions…
www.kirkstall.org
