Copyright © 2004 John Wiley & Sons, Ltd. Biomed. Chromatogr. 18: 408 (2004)

408 Book reviewBOOK REVIEW

Copyright © 2004 John Wiley & Sons, Ltd.

BIOMEDICAL CHROMATOGRAPHYBiomed. Chromatogr. 18: 408 (2004)

Book review

BOOK REVIEW

spectrometry are described in an easy to follow fashion.General data and advice is given on buffers, avoid-ance of proteolysis, concentrations of extracts and bulkpurification. Protein purification protocols present anexcellent set of experimental procedures and a usefullaboratory reference. Authors introduce theory andbackground for each technique, along with lists ofequipment and reagents required to execute these pro-cedures. Advice on potential problems and pitfalls witheach technique is well documented. In summary, Pro-tein Purification Protocols, whether as a laboratorycompanion or reference guide, is ideal for researchersin pursuit of protein purification.

Naveed Ahmed KhanSchool of Biological and Chemical Sciences,

Birkbeck College University of London,London WC1E 7HX, UK

[DOI: 10.1002/bmc.403]

Protein Purification Protocols, 2nd edn, edited byPAUL CUTLER (Methods in Molecular Biology,Volume 244, Series Editor J. M. WALKER). HumanaPress, Totowa, NJ, 2003, US$115.00, 484 pp. ISBN:1-58829-067-0

Protein Purification Protocols is a detailed and com-prehensive collection of protein purification tech-niques. This book provides an up-to-date protocolthat is invaluable to newcomers and of vast benefitto established researchers/scientists. This book encom-passes protocols used on a regular basis by researchersfrom initial fractionation and isolation steps through toseparation by various chromatographic techniques andfinal ‘clean-up’ procedures. Extraction and purificationof proteins from a variety of sources are well described,ranging from proteins of animal and plant tissues torecombinant proteins from bacteria and fungal pro-teins. Analytical techniques of electrophoresis and mass