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Technical Note
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Pro l i fe ra t ion o f HepG2 ce l ls on BRANDpla tes ® ce l lGrade™ p lus sur face
A Metabolic activity measured by resazurin-resafurin turn over is used for relative quantification of cell numbers after 2 and 3 days post seeding. HepG2 cells were incubated in presence of 50µm resazurin for 3 hours prior to fluorescence measurement (Ex 506nm/Em 635nm) in a plate reader (GeminiEM Molecular Devices). HepG2 cells cultivated on BRANDplates® cellGrade™ plus show higher fluorescence signals indicating higher cell numbers after 2 and 3 days in vitro (DIV) when compared to non-treated microplates (PS). Resafurin fluorescence measured in cell-free wells was used for background correction. Data represent mean and standard deviation of 8 measurements.
B Representative images of HepG2 cells cultivated on non treated (PS) and cellGrade™ plus treated microplates at corresponding time points (200x magnification).
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Conc lus ion
BRANDplates® with cellGrade™ plus surface perfectly support attachment and proliferation of HepG2 cells.
Cul ture cond i t ionsFor each experiment HepG2 cells were seeded at a density of 6000 cells/cm2
in wells of transparent 96-well F-bottom BRANDplates® (781602) and cultivated in DMEM medium containing 7% FCS at 37° C, 95% relative humidity and 5% CO2.
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