Challenges

Learn more about Immusoft at http://immusoft.com/

ChemoMetec A/S | Gydevang 43, DK-3450 Allerød, Denmark | Tel: +45 48131020 | Fax: +45 48131021 | mail: contact@chemometec.com | www.chemometec.com

Immusoft’s mission is to develop a breakthrough autologous cell therapy platform for treating a variety of human diseases through a proprietaryImmune System Programming (ISP™) technology. The ISP™ technology can effectively re-programme a patient’s own cells to become miniature drug factories in the body.

This hybrid cell/gene therapy approach uses a clinically validated, non-viral vector for safe, reliable insertion of functional genes into immune cells using the Sleeping Beauty (SB) transposon system.

Immusoft’s autologous cell therapy platform modifies human B cells to become powerful secretory cells for in vivo drug delivery applications. With any cell-based therapeutic a central challenge is the ability to assess, accurately and quickly, the number of cells of interest in each sample and quantitate their viability. The ISP programmed cells are infused back into the patient to produce therapeutic proteins for extended periods

memory. If you get a vaccine, your body remembers it and suddenly the disease that used to kill you won’t even give you the sniffles.… So we really

Matthew Scholz, Founder

“We use ChemoMetec’s NC-200™ high precision automated cell counand accurate standalone instrument. We use the NC-200™ towardsdevelopment of cell viability release tests for our modified B c

Bring unrivalled precision and accuracy to your R&D, QC and manufacturing cell counts

Programming patient cells to cure disease

User Commentary

The NC-200™ is the only automated cell counter on the market that does not require regular maintenance or inter-instrument calibration. Each instrument is precisely calibrated to our master instrument before delivery and requires no future recalibration. To demonstrate this unique capability we have compared five different NC-200s at five different users.

Using serial dilutions ranging from 4x106 – 0.125x106 cells/ml, we show that the linearity of these counts all have R2 values > 0.997. In addition, the inter-instrument variation has CV values below 10% for most cell concentrations with the majority being less than 5%. Even at the lowest concentrations, the CV is less than or similar to that expected from a Poisson probability distribution.

Regression Analysis and Average Values- see next page.

Introduction

Customer Testimonial

©2019 ChemoMetec |

ChemoMetec A/S | Gydevang 43, DK-3450 Allerød, Denmark | Tel: +45 48131020 | Fax: +45 48131021 | mail: contact@chemometec.com | www.chemometec.com

obtain the R2 value.

Figure 1:

Figure 2:

Bring unrivalled precision and accuracy to R&D, QC and Manufacturing Cell Counts

The NucleoCounter® NC-200™ is a high precision automated cell counter utilizing state-of-the-art image cytometry. With user adaptable protocols and specialized assays for aggregated cells and cells on microcarriers, the NC-200™ provides an all-in-one solution for cell counting and cell viability determinations.

The NC-200 is the only automated cell counter on the market that does not require regular maintenance or inter-instrument calibration. Each instrument is precisely calibrated to our master instrument before delivery. No future recalibration is required. To demonstrate this unique capability we have compared five different NC-200s at five different users.

Using serial dilutions ranging from 4x106– 0.125x106 cells/ml, we show that the linearity of these counts all have R2 values > 0.997. In addition, the inter-instrument variation has CV values below 10% for most cell concentrations with the majority being less than 5%. Even at the lowest concentrations, the CV is less than or similar to that expected from a Poisson distribution.

The NC-200™ is also designed to limit human interference in counting. The Via1-Cassette™ includes an in-built pipette and the immobilized fluorescent dyes, acridine orange and DAPI, automatically stain the total and dead cell populations respectively. As a result, all errors introduced during pipetting and staining are avoided.

Dilution 1 Dilution 2 Dilution 3 Dilution 4 Dilution 5 Dilution 6

1 4.22E+106 1.57E+05 3.722 4.26E+106 1.23E+05 2.893 4.21E+106 1.13E+05 2.684 4.19E+106 6.91E+04 1.655 4.24E+106 1.11E+05 2.62

Operator Mean SD CV

1 2.18E+06 4.44E+04 2.042 2.18E+06 4.88E+04 2.233 2.13E+06 6.98E+04 3.274 2.16E+06 7.19E+04 3.335 2.15E+06 6.52E+04 3.02

Operator Mean SD CV

1 1.08E+06 1.64E+04 1.522 1.10E+06 1.30E+04 1.193 1.06E+06 4.91E+04 4.644 1.08E+06 3.03E+04 2.815 1.09E+06 2.70E+04 2.49

Operator Mean SD CV

1 5.41E+05 2.55E+04 4.722 5.52E+05 2.06E+04 3.733 5.18E+05 1.33E+04 2.584 5.37E+05 2.32E+04 4.325 5.24E+05 1.64E+04 3.12

Operator Mean SD CV

1 2.49E+05 2.60E+04 10.442 2.57E+05 4.30E+03 1.673 2.49E+05 1.75E+04 7.024 2.48E+05 1.83E+04 7.365 2.44E+05 8.53E+03 3.50

Operator Mean SD CV

1 1.17E+05 1.08E+04 9.292 1.07E+05 6.67E+03 6.253 1.09E+05 1.28E+04 11.774 1.04E+05 1.29E+04 12.26 5 1.18E+05 9.01E+03 7.65

Operator Mean SD CV

User 1 User 2 User 3 User 4 User 5

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