Proficiency Testing FINAL REPORT Extension …FINAL REPORT Extension Pathogens 16XP2 2016 October Proficiency Testing Provider Certificate Number 3189-02. Program Coordinator: Ingrid

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This report is only for the use of participating laboratories

Proficiency Testing FINAL REPORT

Extension Pathogens

16XP2 2016 October

Proficiency Testing Provider Certificate Number 3189-02.

Program Coordinator: Ingrid Flemming IFM Quality Services Pty Ltd PO Box 877 Ingleburn NSW 2565 Australia Tel: +61 (0)2 9618 3311 Fax: +61 (0)2 9618 3355 Samples Dispatched: 10 October 2016 Results Due: 7 November 2016 Report Issued: 22 November 2016



Operational Summary

Number of rounds and frequency of testing 2 rounds are provided per year.

Sample presentation and reconstitution Samples were presented as freeze-dried vials. Prior to testing, samples were to be reconstituted in accordance with the instructions provided. The final sample volume of 160 mL was to be treated as the “neat” sample.

Assurance of sample integrity Samples were prepared by the Support Division of IFM Quality Services. Homogeneity tests were conducted by the Testing Division of IFM Quality Services. On production, each sample batch was subjected to a homogeneity evaluation. Following initial acceptance, retained samples underwent regular monitoring to confirm ongoing homogeneity and fitness for purpose. All required homogeneity criteria were met.

Method of dispatch All samples were sent via express courier. Insulated packaging and a cooling agent were used to assist in protection against temperature fluctuations during transit.

Assessment procedure Please refer to IFM’s Policies on the assessment of microbiological PTP results:

http://www.ifmqs.com.au/proficiency/references/QPL001-04_Assessment_of_Microbiological_PT_Results.pdf

Participants with outliers are advised to consider their measurement uncertainty and customer expectations before determining whether corrective actions are necessary.

Dispute procedure Participants wishing to dispute their assessment, or appeal an evaluation on other grounds should submit their appeal in writing to the program coordinator.

Please refer to http://www.ifmqs.com.au/ifm_terms_and_conditions.htm for the detailed policy.

Confidentiality The identity of participants and any identifying information supplied by participants is treated as confidential. Appropriate policies and procedures have been put into place to ensure this confidentiality is maintained. Please refer to http://www.ifmqs.com.au/ifm_policy_on_confidentiality.htm for the detailed policy.

Test instructions Instructions applicable to this testing round may be found at:

http://www.ifmqs.com.au/proficiency/instructions/16XP2_Instructions.pdf

http://www.ifmqs.com.au/proficiency/references/QPL001-04_Assessment_of_Microbiological_PT_Results.pdf

http://www.ifmqs.com.au/ifm_terms_and_conditions.htm

http://www.ifmqs.com.au/ifm_policy_on_confidentiality.htm

http://www.ifmqs.com.au/proficiency/instructions/16XP2_Instructions.pdf



Individual results Should individual test results be required, these will be provided upon request.

Available Tests Tests Assessment and Acceptance Criteria

Detection (per 25g) Campylobacter spp. Salmonella spp. Vibrio cholerae Vibrio parahaemolyticus Vibrio (other species) Yersinia enterocolitica Yersinia spp.

Consensus results are used together with the confirmed content of samples. Results are assessed when ≥75% participant consensus is reached.

Identification Campylobacter species Salmonella species Vibrio species Yersinia species

Pass / Fail assessments.

Variations to the normal assessment procedure will be detailed in the discussion.

Participation Summary 29 organisations enrolled 26 organisations participated 38 sets of results were submitted 436 individual test results were received 369 results were assessed 67 results were not assessed 11 results were assessed as outliers

Sample Content Sample A RM Number Organism Name (IFM Number) RM1251 Yersinia enterocolitica SG1 (2801) RM2062 Campylobacter coli (2460) Sample B RM Number Organism Name (IFM Number) RM1625 Campylobacter coli (2460) RM2148 Vibrio parahaemolyticus (5701) RM2269 Vibrio cholerae (5702) Sample C RM Number Organism Name (IFM Number) RM1174 Salmonella Hofit (2318) RM2148 Vibrio parahaemolyticus (5701)



Discussion Generally all participants performed well in this program.

DETECTION AND IDENTIFICATION OF VIBRIO (OTHER) SPP. SAMPLE B AND C

Sample B contained Vibrio parahaemolyticus and Vibrio cholerae. Sample C only contained Vibrio parahaemolyticus. Approximately 50% of participants for sample B and 35% for sample C reported Vibrio species as false positives.

Participants are directed to the PT instructions for the program which states that "Detection of Vibrio (other species) means those species EXCLUDING V. cholerae and V. parahaemolyticus “. The participants who reported the false positive results were not formally assessed but are encouraged to perform appropriate corrective actions in order to prevent a reoccurrence.

DETECTION OF VIBRIO PARAHAEMOLYTICUS IN SAMPLE B

Sample B contained Vibrio parahaemolyticus. IFM’s most recent monitoring indicated that its concentration was <10 cfu/g and consequently may have been below the detection limit for some laboratories. Only 1 participant reported Vibrio parahaemolyticus to be present. As the consensus was that Vibrio parahaemolyticus was absent, all participants received pass assessments.

IDENTIFICATION OF SALMONELLA SPP. IN SAMPLE C

Sample C contained Salmonella Hofit. 2 participants reported the isolate as Salmonella subsp. I rough. While these participants did not identify the serovar, they did correctly identify the subspecies and received pass assessments.



Results

Detection of Campylobacter spp. Sample A (per 25g)

0

5

10

15

20

25

30

Present Absent Other

16XP2 eXtension PathogensDetection of Campylobacter spp.

Sample A (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Campylobacter spp. Sample B (per 25g)

0

5

10

15

20

25

30



Sample B (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Campylobacter spp. Sample C (per 25g)

0

5

10

15

20

25

30



Sample C (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Salmonella spp. Sample A (per 25g)

0

5

10

15

20

25


16XP2 eXtension PathogensDetection of Salmonella spp.

Sample A (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Salmonella spp. Sample B (per 25g)

0

5

10

15

20

25



Sample B (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Salmonella spp. Sample C (per 25g)

0

5

10

15

20

25



Sample C (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Vibrio cholerae Sample A (per 25g)

0

2

4

6

8

10

12

14

16

18

20


16XP2 eXtension PathogensDetection of Vibrio cholerae

Sample A (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Vibrio cholerae Sample B (per 25g)

0

2

4

6

8

10

12

14

16

18

20



Sample B (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Vibrio cholerae Sample C (per 25g)

0

2

4

6

8

10

12

14

16

18

20



Sample C (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Vibrio parahaemolyticus Sample A (per 25g)

0

2

4

6

8

10

12

14

16

18

20


16XP2 eXtension PathogensDetection of Vibrio parahaemolyticus

Sample A (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Vibrio parahaemolyticus Sample B (per 25g)

0

2

4

6

8

10

12

14

16

18

20



Sample B (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Vibrio parahaemolyticus Sample C (per 25g)

0

2

4

6

8

10

12

14

16

18

20



Sample C (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Vibrio (other species) Sample A (per 25g)

0

2

4

6

8

10

12

14


16XP2 eXtension PathogensDetection of Vibrio (other species)

Sample A (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Vibrio (other species) Sample B (per 25g)

0

1

2

3

4

5

6

7

8



Sample B (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Vibrio (other species) Sample C (per 25g)

0

1

2

3

4

5

6

7

8

9

10



Sample C (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Yersinia enterocolitica Sample A (per 25g)

0

1

2

3

4

5

6

7

8

9


16XP2 eXtension PathogensDetection of Yersinia enterocolitica

Sample A (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Yersinia enterocolitica Sample B (per 25g)

0

1

2

3

4

5

6

7

8

9

10



Sample B (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Yersinia enterocolitica Sample C (per 25g)

0

1

2

3

4

5

6

7

8

9

10



Sample C (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Yersinia spp. Sample A (per 25g)

0

1

2

3

4

5

6

7


16XP2 eXtension PathogensDetection of Yersinia spp.

Sample A (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Yersinia spp. Sample B (per 25g)

0

1

2

3

4

5

6

7

8



Sample B (per 25g)

Freq

uenc

y

Excluded

Included



Results

Detection of Yersinia spp. Sample C (per 25g)

0

1

2

3

4

5

6

7

8



Sample C (per 25g)

Freq

uenc

y

Excluded

Included



Results Each table below indicates the number of participants reporting the specified result.

Identification of Campylobacter spp. Identification Sample A Sample B Sample C Campylobacter coli 10 10

Identification of Salmonella spp. Identification Sample A Sample B Sample C Salmonella subsp. I rough 2

Identification of Vibrio spp. Identification Sample A Sample B Sample C Vibrio parahaemolyticus 0 7 Vibrio cholerae 6 0 V.alginolyticus 1 0

Identification of Yersinia spp. Identification Sample A Sample B Sample C Yersinia enterocolitica 1



�End of Report�

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Proficiency Testing FINAL REPORT Extension …FINAL REPORT Extension Pathogens 16XP2 2016 October Proficiency Testing Provider Certificate Number 3189-02. Program Coordinator: Ingrid