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REFERENCES

NANOBIOCONJUGATES FOR SIGNAL AMPLIFICATION IN ELECTROCHEMICAL GENOSENSORS

SEBASTIAN CAJIGAS & JAHIR OROZCO*Max Planck Tandem Group in Nanobioengineering, University of Antioquia

1. Alzate et al. Genosensor for differential detection of Zika virus, Talanta. 210 (2020) 120648.2. Cajigas et. al. Gold/DNA-based nanobioconjugates for ultrasensitive detection of zika genetic material.Microchimical Acta. Submitted, 2020.

*Grupotandem.nanobioe@udea.edu.co

NANOBIOCONJUGATES

Sandwich-like genosensor

ABSTRACT

METHODS AND RESULTS

CONCLUSION & PERSPECTIVE

➢ The approach demonstrated to be useful for the detection of viral RNA, at levels of clinical relevance and holds the potential for the development of electrochemical biosensors to fight pandemics.

Figure 2. Electrochemical genosensor nanobioconjugate-based platform forultrasensitive detection of RNA form the Zika virus A) Schematic representation of theplatform, B) Formation of hierarchical AuNP, C) DPV signal, D) Resultant calibrationcurve (with synthetic DNA) C) Specific detection of Zika RNA in real samples anddiscrimination from other related arbovirus. **level of statistical significance of 99%

Figure 1. A) genosensor assembly B) Magneticisolation onto SPCE C) Sample detection bychronoamperometry D) Signal response E)Calibration curve and LOD.

r2 = 0.9956

LOD = 3 pM

GENOSENSOR DEVELOPMENT

SIGNAL AMPLIFICATION

D

ED

A

E

B

Nanobioconjugates are hybrid materials that result from thecoalescence of biomolecules onto nanomaterials surfaces.They have demonstrated the ability to dramatically increasethe signal response of a biorecognition event whenassembled in biosensors .This work aims to show a generalstrategy for the design of a nanobioconjugate for theamplification of the electrochemical signal recorded ingenosensors for the detection of viral infections.

AuNPs Washing Suspension

METHODOLOGY

DNA reduction

C

C