BiotechnologyBIT-110 (3 hrs)

Dr. Hajra Sadia (PhD, Biotechnology)

Course Outline• Fundamentals of biotechnology• Core Techniques in Biotechnology• DNA Technology

– From DNA to proteins– Principals of recombinant DNA

technology• Microbial Biotechnology

– Products from microorganisms– Fermentation: principal and

application– Food biotechnology

• Plant Biotechnology– Plant tissue culture and

applications– Genetically Engineered Plants– Applications of Plant genetic

engineering– Biofertilizers

Animal BiotechnologyAnimal cell culture and characterization of cell linesApplication of animal cell cultureTransgenic animal technologyEnvironmental BiotechnologyBioremediationPhytoremediationUtilization of biomassBiomining and bioleachingBiotechnology in medicine and health careGene therapyXenotransplantationForensic BiotechnologyHuman Genome ProjectImpacts of biotechnology on human beings: Biotechnology Ethics

Recommended Books

1. Biotechnology By Bourgaize et al. (2003)

2. Basic Biotechnology 3rd ed. By Ratledge and Kristiansen (2008)

3. Biotechnology: Principles and Applications By Rastogi (2007)

4. Biotechnology: An introduction By Ignacimuthu (2008)

Marks distribution

First 1 hr Exam 20 %

Second 1 hr Exam 20 %

Assignment 5 %

Quiz 5 %

Terminal Exam 50 %

What is Biotechnology?

Biotechnology: An Introduction

What is Biotechnology ?bios = lifeteuchos = toollogos = study of or essence ofe.g. the study of tools from living things/organisms

Classical definition “Biotechnology is a set of tools that utilize living things

(and more recently, derivatives of living things) to solve problems or to provide products”

What is Biotechnology ?The application of various sciences (i.e., immunology, molecular biology, biochemistry, botany, animal science, etc.) to develop products or to solve problems.

• Technology Assessment of the U.S. Congress defines biotechnology as

"any technique that uses living organisms or their products to make or modify a product, to improve plants or animals, or to develop microorganisms for specific uses."

Utilization of a biological process, be it microbial, plant or animal cells or their constituents to provide goods and services to mankind.

Goods: Products of industries concerned with food, beverages, pharmaceutical, biochemicals and winning of metals

Services: Largely concerned with water purification, industrial and domestic waste management including sewage water

What is Biotechnology ?

The Promise of Biotechnology Diagnosing disease Curing disease Nutritious food/feed Healthy food/feed Productive land Feeding the poor Sustainable agriculture Healthy environment

History of BiotechnologyThe term "biotechnology" was coined in 1919 by Karl Ereky, Hungarian engineer

All lines of work by which products are produced from raw materials with the aid of living things

RawMaterial

Upstream Processing

Fermentation andBiotransformation

DownstreamProcessing

PureProduct

• Traditional biotechnology has been used for thousands of years to produce improved food and health care products.

• Modern biotechnology enables us to develop improved products more safely and more rapidly than ever before.

• Biotechnology in one form or another has flourished since prehistoric times.

Examples…They could plant their own crops and breed their own animals, they learned to use biotechnology.The discovery that fruit juices fermented into wine, or that milk could be converted into cheese or yogurt, or that beer could be made by fermenting solutions of malt and hops began the study of BiotechnologyWhen the first bakers found that they could make a soft, spongy bread rather than a firm, thin cracker, they were acting as fledgling biotechnologists.The first animal breeders, realizing that different physical traits could be either magnified or lost by mating appropriate pairs of animals, engaged in the manipulations of biotechnology.

Recombinant genetic engineering……….Using biological process to develop products

G. Steven Burrill 1997

Modern Biotechnology

Why Biotechnology?

Bioproduction of drugs so complex they can only be synthesized in a living system

Origins of Biotechnology• Historical pharmaceutical biotechnology:

– Alexander Fleming discovery of penicillin from bread mold - 1928

– Large scale broth tank production of penicillin for injuries – Florey & Chain

• Modern pharmaceutical biotechnology:– interspecies genetic transplantation– hybridoma – tumor cell and leukocyte fusions– heterologous protein production (microbes,

animal and plant cells)

Biotechnology Timeline1750 B.C. Sumerians use yeast to brew beer500 B.C. Chinese use mold as an antibiotic to treat boils1863 Mendel discovers transmission of genetic traits1906 First early study of genes; term “genetics”

introduced1919 Term “biotechnology” first used by agriculturalist1928 Penicillin discovered1953 Watson and Crick discover double-helix structure

of DNA1960 First synthetic antibiotic

Biotechnology Timeline (Cont…)1963-64 The Green Revolution (dwarf wheat with 70%

increased yield through hybridization)1965 Mouse-human cells successfully fused1966-68 Genetic code cracked1971 First complete synthesis of a gene1971 Restriction enzyme discovered (EcoRI)1973-78 Recombinant DNA technology to cut and paste genes1975 DNA sequencing1975 Hybridoma technology (monoclonal antibodies)1978 Insulin gene cloned1980-88 Live organism patented (Engineered mice)

Biotechnology: last 30 Years1981 First transgenic animal1982 First biotech drug marketed (Insulin)1983 First transgenic plant (tobacco) 1983 First artificial chromosome1985 Genetically engineered plants field tested1986 Use of microbes to clean up oil spill1986 Polymerase chain reaction by Kary Mullis,

revolutionize molecular biology (Nobel prize 93)1988 First patent for genetically altered animal

(transgenic mouse)1995 First non-viral full gene sequence

completed

Biotechnology: last 20 Years (Cont…)1996-98 First mammal cloned (Dolly the cloned sheep unveiled)2000 First entire plant genome sequenced (Arabidopsis

thaliana)2001 Cloning banned (the US house of representatives passes

the human cloning prohibition act of 2001, a ban on all human cloning2002 Mapping of human genome virtually complete2002-date Rethinking of RNA (scientists are forced to rethink their view of RNA when they discover how important small pieces of RNA are in controlling many cell functions 2005 Human genome confirmed

Crops Drugs Vaccines Diagnostic Livestock Environment

MolecularBiology

Microbiology Biochemistry Genetics ChemicalEngineering

Cell Biology

BIOTECHNOLOGY

Biotechnology is an integrated use of biochemistry, microbiology and engineering sciences to achieve technological applications of the capabilities of microbes, cultured tissue cells and parts thereof for production of value added products.

Biotechnology

Applications• Biotechnology has applications in four

major industrial areas, including – health care (medical), – crop production and agriculture, – non food (industrial) uses of crops and other

products (e.g. biodegradable plastics, vegetable oil, biofuels), and

– environmental uses.

Branches of Biotechnology

Bioinformatics• An interdisciplinary field which addresses biological

problems using computational techniques, and makes the rapid organization and analysis of biological data possible.

• The field may also be referred to as computational biology, – conceptualizing biology in terms of molecules and then

applying informatics techniques to understand and organize the information associated with these molecules, on a large scale.

• Bioinformatics plays a key role in various areas, such as functional genomics, structural genomics, and proteomics, and forms a key component in the biotechnology and pharmaceutical sector.

Blue biotechnology • Blue biotechnology is concerned with the

application of molecular biological methods to marine and freshwater organisms. – It involves the use of these organisms, and

their derivatives, for purposes such as increasing seafood supply and safety, controlling the proliferation of noxious water-borne organisms, and developing new drugs.

Green biotechnology • Biotechnology applied to agricultural processes.

– Selection and domestication of plants via micropropagation.

– Designing of transgenic plants to grow under specific environments in the presence (or absence) of chemicals.

Green biotechnology might produce more environmentally friendly solutions than traditional industrial agriculture.

– Engineering of a plant to express a pesticide, thereby ending the need of external application of pesticides. • E.g. Bt corn.

Red biotechnology • application of biotechnology in the

medicine and human healthcare sector.– Therapeutic proteins– Designing of organisms to produce

antibiotics, and – Engineering of genetic cures through

genetic manipulation.

White biotechnology• Also known as industrial biotechnology, is

biotechnology applied to industrial processes. – Designing of an organism to produce a useful

chemical. – Use of enzymes as industrial catalysts to either

produce valuable chemicals or destroy hazardous/polluting chemicals.

• White biotechnology tends to consume less in resources than traditional processes used to produce industrial goods.

Applications of Biotechnology:

Food Industry Pharmaceutical Industry Chemical Industry Enzyme Industry Mining Industry Agricultural Industry Environmental Industry

THANKS