PLANT RESPONSE BIOTECH S.L.

- R+D Biotechnology company - Spin-off from the Biotecnología Department/Centro de Biotecnología y Genómica de Plantas (CBGP) of the Universidad Politécnica de Madrid.

Mission and vision

- Transfer excellence science results to the farmers -Answer plant protection and breeding market needs developing new technologies compatible with intensive, integrated and ecological Agriculture

-Reduce agriculture’s environmental impact and improve our quality of life with healthier food without residues

PlantResponse

PlantResponse

Organization

Board of directors Scientific advisory board

Dr. John Ryals Metabolon Inc, Research Triangle Park, North

Carolina, USA

Dra. Salome Prat Research Professor, CNB-CSIC, Madrid, Spain

Dr. Dierk Sheel Director Leibniz Institute of Plant Biochemistry

(IPB), Halle, Germany

Management team

Collaborations Thorsten Nürnberger , Jürg Felix, Frederik Brunner, Andrea Gust (EKUTübingen) Ivo Feussner (University of Götinguen) Cyril Zypfel (The Sainsbury Laboratory in Norwich) Julio Salinas (CIB-CSIC)

Co- founder

Dr. Antonio

Molina

CEO

Eduardo Quemada

R&d Head Manager

Dr. Marisé

Borja

New technologies (Know-how and Patents) to control biotic or abiotic stress in plants, are

developed in collaboration through licence with Research Centers or companies to bring the following

products to the market:

* “New stimulators/elicitors” activating plant innate immunity in plants from:

- Natural sources: microorganism or plant derived molecules

- Chemical sinthesis: Chemetics (Chemical Genetics) identification

* Plant metabolites activating plant stress resistance

* Plant Genes coding for the proteines involved in the elicitor recognition, plant stress signaling or

down stream components to breed new plant varieties resistant to biotic and abiotic stresses by:

- Classical breeding (horticultural species)

- Biotechnology(GMOs for extensive crops corn, soy bean, potato,.. )

Plant Response Biotech Technologies and Products

MAMP/ELICITORS

RLK

Immune Response and Resistance to Pathogens

RLK

RLK

Plant Cell

Pathogens

PK

PK

TIR

PK

(PRRs)

K

LRR

DROSOPHILA

TOLL LR

R

TIR

MAMMALS

TLR4

LBP

LR

R

CD14

PLANTS

Xa21

K

LR

R

FLS2

K

LR

R

RPS5

BAK1

K

EFR

K

LR

R

MAMPs

flg22 Spätzle

PGN

LPS elf18

LR

R

NBS

LR

R

NBS CC

TIR

RPS4

LRR

NB

S

Avr

PphB

Avr

Xa21

Avr

Rps4

Effectors

TIR

PBS1

IMMUNE RESPONSE GENES

ANTIMICROBIAL PEPTIDES and PR (plant defence-related) proteins

Dif/

Relish

Cactus

TFs

(WRKYs/TGAs) NF-kB

IkB

MYD88 PK Tube PK

IRAK

MAPKs PK

PK

K

PK

KK

PK

PK

K

PK

KK

PK

PK

K

PK

KK

Pelle

MEKK1

MEK1/MKK2

MPK4

MEKK?

MKK4/MKK5

MPK3/MPK6

PlantResponse

Plant Innate immunity applied to biotic stress response

Tena et al, 2011

Plant Innate immunity applied to biotic stress response

High throughput screening for conserved responses High throughput screening

Tena et al, 2011

Plant Innate immunity applied to biotic stress response

High throughput screening for early responses

PlantResponse

Field/greenhouse trials

PBR1 product development

PlantResponse

Garlic field trials: 10-20% production increase

PBR1 product development

0

20

40

60

80

100

120

CONTROL Product A Product B PRB1 2g/l PRB1 4g/l CONTROL PRB1 2g/l PRB1 4g/l

Wei

gh

t (g

ram

s)

Treatment

Fresh weight 2012

0

10

20

30

40

50

60

CONTROL

(PRELUDE)

PRB1

(2g/L)

PRB1

(4g/L)

Product C

(Bion min)

Product C

(Bion max)

Dry weight 2009)

Pepper greenhouse trials: 10-20% production increase

PBR1 product development

0

2

4

6

8

10

12

14

ORIUS

SWIRSKII

It does not affect biological control insects and production is 1 month earlier

Kil

ogra

ms/

trea

tmen

t

PRB1 Product Development

Organic onion production: 20-30% production increase

*

*

We

igh

t

PBR1 TREATMENT

MEANS and Fisher 95% LSD

QUALITY CONTROL: The company has done extensive formulation

and quality control validation trials since there is a big variability among

different sources and formulations. Extensive data are available for the

PRODUCT REGISTRATION.

PlantResponse

Product Development QUALITY CONTROL

0

200

400

600

800

1000

1200

1400

0 7…

1…

2…

2…

3…

4…

4…

5…

6…

7…

7…

8…

RL

U

PBR3 ESP 10mM

Aequo

Col 0

Aequo+H2O

0

200

400

600

800

1000

1200

1400

0

68

138

210

282

354

426

498

570

642

714

786

858

RL

U

PBR3 AS12 50mM

Door

1 m

9

8 64 m2

Pathology

lab

96 m2

Working

station

Product Development GROWER TRIALS

CROPS STRESS

Tomato Pepper Melon Potato Rice Canola Citrus Grapevine Strawberry Ornamental

Xanthomonas axonopodis pv. citri

X X X

Sclerotinia sclerotiorum X

Phytophthora infestans X

Fusarium oxysporum X X

Botrytis cinerea X X X X X

Magnaporte oryzae X

Rhizoctonia solani X X X X X

Rhizopus oryzae X

Ralstonia solanacearum X X X

Drought X X X X

Salinity X X X X X

Frost X X X X X

Plant stress response Current Targets

X: ongoing X: planned

PlantResponse

From the lab to the grower: Solutions for food and agriculture challenges