Picria fel-terrae Lour

114

Lampiran 1. Hasil identifikasi daun poguntano (Picria fel-terrae Lour.)

Universitas Sumatera Utara

115

Lampiran 2 Simplisia daun poguntano (Picria fel-terrae Lour.)

A

a

b

Keterangan:

a. Gambar daun poguntano

b. Gambar simplisia daun poguntano


116

Lampiran 3. Mikroskopik serbuk simplisia daun poguntano (perbesaran 10x10)

Keterangan:

a. Sel epidermis

b. Trikoma (rambut penutup)

c. Kristal kalsium oksalat (bentuk prisma)

d. Tulang daun

e. Stomata tipe diasitik

f. Sel tetangga

a

b

c

d

e

f


117

Lampiran 4. : Bagan skrining fitokimia, karakterisasi simplisia dan ekstrak

Dicuci

Ditiriskan

Dikeringkan (diangin-anginkan)

dihaluskan

Daun poguntano

Simplisia

Serbuk Simplisia

Skrining

Fitokimia

Pembuatan Ekstrak

Karakterisasi

Simplisia

- Alkaloid

- Flavonoid

- Saponin

- Tanin

- Glikosida

- Antrakuinon

- Steroid/Triterpenoid

a

- Pemeriksaan mikroskopik

- Pemeriksaan makroskopik

- Penetapan kadar air

- Penetapan kadar sari larut etanol

- Penetapan kadar sari larut air

- Penetapan kadar abu total

- Penetapan kadar abu tidak larut asam

Ekstrak


118

Lampiran 5. Bagan kerja pembuatan ekstrak etanol secara maserasi

Ditambahkan etanol 96%,

dimaserasi selama 3 hari

Serbuk simplisia

daun poguntano

Maserat Ampas

Maserat 1 Ampas

Maserat 2 Ampas

Ekstrak etanol

(Krud ekstrak)

Diamkan 2 hari

Dimaserasi kembali

dengan etanol 96% 3

hari

Dienaptuangkan

Diremaserasi

dengan etanol

3 hari

Dipekatkan dengan

rotary evaporator

Dikeringkan dengan

freeze dryer

Sisa Maserat

Disaring


119

Lampiran 6. Bagan ekstraksi serbuk simplisia secara maserasi bertingkat

dipekatkan

diskrining

di KLT

j

Keterangan : * = Uji Aktivitas Antidiabetes

Serbuk simplisia

Ampas Maserat

Ampas Maserat

Ekstrak n-heksana*

Ampas

Maserat

Ekstrak etilasetat*

Kromatogram

Ekstrak etanol*

Hasil

Hasil

di maserasi

dengan etanol

3x

diskrining

diskrining

dipekatkan

dimaserasi dengan

etilasetat 3x

Dimaserasi dengan

n-heksana 3x

dipekatkan


120

Lampiran 7. Perhitungan kadar air simplisia dan ekstrak daun poguntano

(Picria fel-terrae Lour.)

% Kadar air simplisia =

x 100%

No. Berat sampel (g) Volume awal (ml) Volume akhir (ml)

1. 5,018 1,90 2,20

2. 5,039 2,20 2,50

3. 5,010 2,50 2,80

% Kadar air =

x 100%

1. Kadar air =

x 100% = 5,98%

2. Kadar air =

x 100% = 5,95%

3. Kadar air =

x 100% = 5,98%

% Rata-rata kadar air =

= 5,97%

Perhitungan kadar air ekstrak n-heksana daun poguntano (Picria fel-terrae Lour.)

% Kadar air simplisia =

x 100%

No. Berat sampel (g) Volume awal (ml) Volume akhir (ml)

1. 2,018 1,90 2,00

2. 2,039 2,00 2,10

3. 2,010 2,10 2,20

% Kadar air =

x 100%

1. Kadar air =

x 100% = 4,96%

2. Kadar air =

x 100% = 4,90%

3. Kadar air =

x 100% = 4,98%

% Rata-rata kadar air =

= 4,9


121

Lampiran 8. Perhitungan kadar sari larut air simplisia dan ekstrak

daun poguntano (Picria fel-terrae Lour.)

% Kadar sari larut dalam air =

x 100%

No. Berat sampel (g) Berat sari (g)

1. 4,010 0,205

2. 4,004 0,201

3. 4,007 0,211

1. Kadar sari larut dalam air =

x 100% = 25,56%


x 100% = 25,10%


x 100% = 26,33%

% Rata-rata kadar sari larut dalam air =

= 25,66%

Perhitungan kadar sari larut air ekstrak n-heksana daun poguntano


% Kadar sari larut dalam air =

x 100%


1. 1,158 0,002

2. 1,201 0,002

3. 1,125 0,001


x 100% = 0,86%


x 100% = 0,83%


x 100% = 0,44%

% Rata-rata kadar sari larut dalam air =

= 0,71%


122

Lampiran 9. Perhitungan kadar sari larut etanol simplisia daun poguntano


% Kadar sari larut dalam etanol =

x 100%


1. 4,009 0,154

2. 4,010 0,168

3. 4,014 0,143

1. Kadar sari larut dalam etanol =

x 100% = 19,21%


x 100% = 20,95%


x 100% = 17,81%

% Rata-rata kadar sari larut dalam etanol =

= 19,23%

Perhitungan kadar sari larut etanol ekstrak n-heksana daun poguntano


% Kadar sari larut dalam etanol =

x 100%


x 100% = 2,92%

2.Kadar sari larut dalam etanol =

x 100% = 2,88%

3.Kadar sari larut dalam etanol =

x 100% = 2,52%

% Rata-rata kadar sari larut dalam etanol =


1. 1,197 0,007

2. 1,217 0,007

3. 1,189 0,006


123

Lampiran 10. Perhitungan kadar abu total simplisia daun poguntano


% Kadar abu total =

100%

No. Berat sampel (g) Berat abu (g)

1. 2,0251 0,0803

2. 2,0200 0,0820

3. 2,0198 0,0810

1. Kadar abu total =

100% = 3,96%


100% = 4,06%


100% = 4,01%

% Rata-rata kadar abu total =

= 4,01%

Perhitungan kadar abu total ekstrak n-heksana daun poguntano


% Kadar abu total =

100%


1. 1,0047 0,0041

2. 1,0033 0,0065

3. 1,0109 0,0034


100% = 0,41%


100% = 0,65%

3.Kadar abu total =

100% = 0,34%


= 0,47%


124

Lampiran 11. Perhitungan kadar abu tidak larut asam simplisia

daun poguntano (Picria fel-terrae Lour.)

% Kadar abu total =

100%


1. 2,0251 0,0155

2. 2,0200 0,0159

3. 2,0198 0,0160


100% = 0,76%


100% = 0,79%


100% = 0,79%


= 0,78%

Perhitungan kadar abu tidak larut asam ekstrak n-heksana daun poguntano


% Kadar abu tidak larut dalam asam =

100%


1. 1,0047 0,0009

2. 1,0033 0,0007

3. 1,0109 0,0003

1. Kadar abu tidak larut dalam asam =

100% = 0,09%


100% = 0,07%


100% = 0,03%

% Rata-rata kadar abu tidak larut dalam asam =

= 0,06%


125

Lampiran 12. Gambar hewan percobaan

Hewan Mencit Jantan Putih


126

Lampiran 13 Data Pengukuran Kadar Glukosa Darah (KGD) Mencit.

(Toleransi Glukosa)

1. KGD mencit setelah pemberian Suspensi Na-CMC 0,5%.

No BB

Mencit

(g)

KGD

Puasa

(mg/dL)

Setelah Pemberian Larutan Glukosa (mg/dL)

Menit 30 Menit 60 Menit 90 Menit 120

1 34,5 92 276 220 155 128

2 31,6 90 285 265 160 129

3 27,1 95 250 235 146 120

4 33,7 90 288 259 160 123

5 34,3 93 280 236 190 130

6 33,2 89 270 260 185 121

Rata-rata 91,5 274,8 245,8 166 125,2

2. KGD mencit setelah pemberian Suspensi Glibenklamid dosis 0,65 mg/kg bb.

No BB

Mencit

(g)

KGD

Puasa

(mg/dL)



1 33,1 94 132 120 100 80

2 34,7 98 179 115 105 71

3 33,2 96 175 128 98 74

4 34,2 88 145 92 107 75

5 37,1 85 162 122 105 79

6 36,0 98 141 103 100 80

Rata-rata 93,2 155,7 113,3 102,5 76,5

3. KGD mencit setelah pemberian CEEDPt dosis 100 mg /kg bb

No BB

Mencit

(g)

KGD

Puasa

(mg/dL)



1 33,7 100 195 162 110 80

2 28,3 92 180 145 121 89

3 31,3 85 177 124 113 90

4 30,0 90 179 135 117 90

5 32,1 94 178 140 102 96

6 29,0 97 169 132 102 97

Rata-rata 93 179,7 139,7 110,8 90,3


127

4. KGD mencit setelah pemberian CEEDPt dosis 200 mg/kg bb.

No BB

Mencit

(g)

KGD

Puasa

(mg/dL)



1 38,5 86 170 136 110 80

2 32,7 95 172 124 109 90

3 28,6 90 175 140 100 78

4 36,2 90 179 133 109 86

5 35,0 87 170 118 99 76

6 30,9 91 178 122 100 85

Rata-rata 89,8 174 128,8 104,5 82.5

5. KGD mencit setelah pemberian CEEDPt dosis 300 mg/kg bb.

No BB

Mencit

(g)

KGD

Puasa

(mg/dL)



1 35,9 96 175 141 120 88

2 28,6 90 175 140 126 105

3 35,7 88 170 140 115 98

4 35,2 92 170 135 122 96

5 34,5 89 182 140 130 105

6 35,2 95 175 145 122 98

Rata-rata 91,7 174.5 140’2 122.5 98.3


128

Lampiran 14 Uji Distribusi Normalitas Kolmogorov-Smirnov Oneway

Descriptives

kadar gula darah puasa

N Mean

Std.

Deviation

Std.

Error

95% Confidence

Interval for Mean

Mini

mum

Maxim

um

Lower

Bound

Upper

Bound

cmc 6 91.5000 2.25832 .92195 89.1300 93.8700 89.00 95.00

glibelklamid 6 93.1667 5.45588 2.22736 87.4411 98.8923 85.00 98.00

CEEDPt 100 6 93.0000 5.29150 2.16025 87.4469 98.5531 85.00 100.00

CEEDPt 200 6 89.8333 3.18852 1.30171 86.4872 93.1795 86.00 95.00

CEEDPt 300 6 91.6667 3.26599 1.33333 88.2392 95.0941 88.00 96.00

Total 3

0

91.8333 3.99209 .72885 90.3427 93.3240 85.00 100.00

ANOVA


Sum of Squares Df Mean Square F Sig.

Between Groups 43.667 4 10.917 .652 .631

Within Groups 418.500 25 16.740

Total 462.167 29

Post Hoc Tests Homogeneous Subsets


Duncana

perlakuan N

Subset for alpha = 0.05

1

CEEDPt 200 6 89.8333

cmc 6 91.5000

CEEDPt 300 6 91.6667

CEEDPt 100 6 93.0000

glibelklamid 6 93.1667

Sig. .218

Means for groups in homogeneous subsets are displayed.


129


Duncana

perlakuan N


1

CEEDPt 200 6 89.8333

cmc 6 91.5000

CEEDPt 300 6 91.6667

CEEDPt 100 6 93.0000


Sig. .218


a. Uses Harmonic Mean Sample Size = 6.000.

Oneway

Descriptives

kadar gula darah menit ke 30

N Mean

Std.

Deviation

Std.

Error

95% Confidence

Interval for Mean

Minim

um

Maxim

um

Lower

Bound

Upper

Bound

cmc 6 274.8333 13.74651 5.61199 260.4073 289.2594 250.00 288.00

glibelklamid 6 155.6667 19.22152 7.84715 135.4949 175.8384 132.00 179.00

CEEDPt 100 6 179.6667 8.47742 3.46089 170.7702 188.5632 169.00 195.00

CEEDPt 200 6 174.0000 3.94968 1.61245 169.8551 178.1449 170.00 179.00

CEEDPt 300 6 174.5000 4.41588 1.80278 169.8658 179.1342 170.00 182.00

Total 30 191.7333 44.37608 8.10193 175.1630 208.3036 132.00 288.00

ANOVA



Between Groups 53780.867 4 13445.217 101.031 .000

Within Groups 3327.000 25 133.080

Total 57107.867 29


130



Duncana

perlakuan N


1 2 3


CEEDPt 200 6 174.0000

CEEDPt 300 6 174.5000

CEEDPt 100 6 179.6667

cmc 6 274.8333

Sig. 1.000 .430 1.000



Oneway

Descriptives


N Mean

Std.

Deviation

Std.

Error

95% Confidence

Interval for Mean

Minim

um

Maxim

um

Lower

Bound

Upper

Bound

cmc 6 245.8333 18.01573 7.35489 226.9270 264.7397 220.00 265.00

glibelklamid 6 113.3333 13.41144 5.47520 99.2589 127.4078 92.00 128.00

CEEDPt 100 6 139.6667 13.06395 5.33333 125.9569 153.3764 124.00 162.00

CEEDPt 200 6 128.8333 8.72735 3.56293 119.6745 137.9921 118.00 140.00

CEEDPt 300 6 140.1667 3.18852 1.30171 136.8205 143.5128 135.00 145.00

Total 30 153.5667 49.30740 9.00226 135.1550 171.9784 92.00 265.00

ANOVA



Between Groups 66698.200 4 16674.550 109.494 .000

Within Groups 3807.167 25 152.287

Total 70505.367 29


131



Duncana

perlakuan N


1 2 3


CEEDPt 200 6 128.8333

CEEDPt 100 6 139.6667

CEEDPt 300 6 140.1667

cmc 6 245.8333

Sig. 1.000 .145 1.000



Oneway

Descriptives


N Mean

Std.

Deviation

Std.

Error

95% Confidence

Interval for Mean

Minim

um

Maxim

um

Lower

Bound

Upper

Bound

cmc 6 166.0000 17.49286 7.14143 147.6424 184.3576 146.00 190.00

glibelklamid 6 102.5000 3.61939 1.47761 98.7017 106.2983 98.00 107.00

CEEDPt 100 6 110.8333 7.78246 3.17718 102.6661 119.0005 102.00 121.00

CEEDPt 200 6 104.5000 5.31977 2.17179 98.9172 110.0828 99.00 110.00

CEEDPt 300 6 122.5000 5.12835 2.09364 117.1181 127.8819 115.00 130.00

Total 30 121.2667 25.35259 4.62873 111.7999 130.7335 98.00 190.00

ANOVA



Between Groups 16468.533 4 4117.133 47.403 .000

Within Groups 2171.333 25 86.853

Total 18639.867 29


132

Oneway

Descriptives


N Mean

Std.

Deviation

Std.

Error

95% Confidence

Interval for Mean

Minim

um

Maxim

um

Lower

Bound

Upper

Bound

cmc 6 125.1667 4.35507 1.77795 120.5963 129.7370 120.00 130.00

glibelklamid 6 76.5000 3.72827 1.52206 72.5874 80.4126 71.00 80.00

CEEDPt 100 6 90.3333 6.08824 2.48551 83.9441 96.7225 80.00 97.00

CEEDPt 200 6 82.5000 5.35724 2.18708 76.8779 88.1221 76.00 90.00

CEEDPt 300 6 98.3333 6.34560 2.59058 91.6740 104.9926 88.00 105.00

Total 30 94.5667 17.94375 3.27607 87.8664 101.2670 71.00 130.00

ANOVA



Between Groups 8642.867 4 2160.717 77.780 .000

Within Groups 694.500 25 27.780

Total 9337.367 29

Post Hoc Tests

Homogeneous Subsets


Duncana

perlakuan N


1 2 3


CEEDPt 200 6 104.5000

CEEDPt 100 6 110.8333

CEEDPt 300 6 122.5000

cmc 6 166.0000

Sig. .155 1.000 1.000


a. Uses Harmonic Sample Size = 6.000.Mean


133



Duncana

perlakuan N


1 2 3 4


CEEDPt 200 6 82.5000

CEEDPt100 6 90.3333

CEEDPt300 6 98.3333

cmc 6 125.1667

Sig. .060 1.000 1.000 1.000




134

4. EKSTRAK n-HEKSANA (200 mg/Kg bb)

Lampiran 15. Data kadar gula darah mencit dengan berbagai perlakuan

Berat (gram) KGD Puasa

(mg/dl)

KGD aloksan

(mg/dl)

Hari ke-

3 5 7 10

29,5 83 588 545 505 430 290

28,9 95 590 550 516 424 285

30,2 78 588 550 505 415 276

28,8 83 585 540 500 416 285

29,7 80 592 543 495 432 282

30,1 79 592 538 514 435 288

Berat (gram) KGD Puasa KGD aloksan Hari ke-

3 5 7 10

27,3 83 401 399 410 400 401

26,8 78 412 405 413 405 395

28,1 85 440 429 398 387 380

26,8 83 399 380 397 391 401

27,1 80 408 410 402 400 393

26,9 81 419 395 399 387 395


(mg/dl)

KGD aloksan

(mg/dl)

Hari ke-

3 5 7 10

24,9 83 483 437 344 261 206

24,5 82 483 410 372 229 184

23,8 79 501 411 391 303 180

25,2 78 478 430 365 215 208

25,1 84 477 426 348 231 175

25,3 90 480 449 338 242 198


(mg/dl)

KGD aloksan

(mg/dl)

Hari ke-

3 5 7 10

25,1 82 466 430 368 321 263

24,8 78 476 421 361 311 254

25,3 85 475 434 376 332 270

26,0 83 465 421 367 328 251

24,3 81 466 420 371 312 273

24,8 78 480 427 375 325 259

1. METFORMIN HCL (50 mg/Kg bb)

2. KONTROL (CMC-Na 1%)

3. CRUDE EKSTRAK ETANOL (200 )mg/Kg bb)


135

5. EKSTRAK ETILASETAT (200 mg/Kg bb)


(mg/dl)

KGD aloksan

(mg/dl)

Hari ke-

3 5 7 10

26,9 83 514 482 420 275 237

27,3 79 518 471 433 301 225

28,1 82 513 470 422 276 217

26,5 91 505 495 435 278 241

27,8 79 516 495 422 311 249

29,0 82 510 501 430 289 247


(mg/dl)

KGD aloksan

(mg/dl)

Hari ke-

3 5 7 10

26,2 83 467 412 385 319 231

26,0 77 491 483 373 345 201

25,8 84 488 459 412 380 219

26,8 85 456 452 411 350 220

27,0 74 477 451 398 331 211

25,6 91 499 472 411 356 239

6. EKSTRAK ETANOL (200 mg/Kg bb)


136

Lampiran 15(Lanjutan) Uji Distribusi Normalitas Kolmogorov-Smirnov

Tests of Normalityb,c,d,e,f,g

Perlakuan

Kolmogorov-

Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

Hari 3 Metformin HCl 50 mg/kg bb .133 6 .200* .969 6 .886

Suspensi CMC-Na 1% .181 5 .200* .967 5 .858

Crude Ekstrak Etanol 200 mg/kg bb .256 6 .200* .938 6 .641

Ekstrak n-Heksan 200 mg/kg bb .155 6 .200* .972 6 .907

Ekstrak Etilasetat 200 mg/kg bb .184 6 .200* .901 6 .382

Ekstrak Etanol 200 mg/kg bb .256 6 .200* .897 6 .357


Suspensi CMC-Na 1% .267 5 .200* .875 5 .289


Ekstrak n-Heksan 200 mg/kg bb .305 6 .084 .799 6 .058




Suspensi CMC-Na 1% .346 5 .050 .794 5 .072


Ekstrak n-Heksan 200 mg/kg bb .139 6 .200* .972 6 .906



Hari

10

Metformin HCl 50 mg/kg bb .189 6 .200* .960 6 .821

Suspensi CMC-Na 1% .270 5 .200* .912 5 .477


Ekstrak n-Heksan 200 mg/kg bb .273 6 .185 .896 6 .352



a. Lilliefors Significance Correction

*. This is a lower bound of the true significance.

b. Hari 1 is constant when Perlakuan = Metformin HCl 50 mg/kg bb. It has been omitted.


137

c. Hari 1 is constant when Perlakuan = Suspensi CMC-Na 1%. It has been omitted.

d.Hari 1 is constant when Perlakuan = Crude Ekstrak Etanol 200 mg/kg bb. It has been

omitted.

e. Hari 1 is constant when Perlakuan = Ekstrak n-Heksan 200 mg/kg bb. It has been

omitted.

f. Hari 1 is constant when Perlakuan = Ekstrak Etilasetat 200 mg/kg bb. It has been

omitted.

g. Hari 1 is constant when Perlakuan = Ekstrak Etanol 200 mg/kg bb. It has been

omitted.

Lampiran 15 (lanjutan)

Uji Anova Oneway

Descriptives

N Mean

Std.

Deviati

on

Std.

Error

95% Confidence

Interval for

Mean

Minim

um

Maxim

um

Lower

Bound

Upper

Bound

Hari

1

Metformin HCl 50

mg/kg bb

6 .0000 .00000 .00000 .0000 .0000 .00 .00

Suspensi CMC-Na

1%

6 .0000 .00000 .00000 .0000 .0000 .00 .00

Crude Ekstrak

Etanol 200 mg/kg

bb

6 .0000 .00000 .00000 .0000 .0000 .00 .00

Ekstrak n-Heksan

200 mg/kg bb

6 .0000 .00000 .00000 .0000 .0000 .00 .00

Ekstrak Etilasetat

200 mg/kg bb

6 .0000 .00000 .00000 .0000 .0000 .00 .00

Ekstrak Etanol

200 mg/kg bb

6 .0000 .00000 .00000 .0000 .0000 .00 .00

Total 36 .0000 .00000 .00000 .0000 .0000 .00 .00

Hari

3

Metformin HCl 50

mg/kg BB

6 7.6067 .98354 .40153 6.5745 8.6388 6.46 9.12

Suspensi CMC-Na

1%

6 2.4500 2.4127 .98498 -.0820 4.9820 -.49 5.73

Crude Ekstrak

Etanol 200 mg/kg

bb

6 11.6300 4.1658 1.7007 7.2582 16.0018 6.46 17.96

Ekstrak n-Heksan

200 mg/kg bb

6 9.7133 1.4373 .58678 8.2050 11.2217 7.73 11.55

Ekstrak Etilasetat

200 mg/kg bb

6 5.2483 3.1500 1.2860 1.9425 8.5541 1.76 9.07

Ekstrak Etanol 200

mg/kg bb

6 5.1817 3.8821 1.5848 1.1076 9.2557 .88 11.78

Total 36 6.9717 4.1177 .68629 5.5784 8.3649 -.49 17.96


138

Hari

5

Metformin HCl 50

mg/kg bb

6 14.1467 1.3188 .53842 12.762 15.5307 12.54 16.39

Suspensi CMC-Na

1%

6 2.3017 4.2345 1.7287 -

2.1422

6.7455 -2.24 9.55

Crude Ekstrak

Etanol 200 mg/kg

bb

6 25.6633 3.2236

0

1.3160

3

22.280

4

29.0463 21.96 29.58

Ekstrak n-Heksan

200 mg/kg bb

6 21.5633 1.3609

5

.55561 20.135

1

22.9916 20.39 24.16

Ekstrak Etilasetat

200 mg/kg bb

6 16.7017 1.7372

3

.70922 14.878

6

18.5248 13.86 18.29

Ekstrak Etanol

200 mg/kg bb

6 16.8717 4.5413

8

1.8540

1

12.105

8

21.6376 9.87 24.03

Total 36 16.2081 7.8852

4

1.3142

1

13.540

1

18.8760 -2.24 29.58

Hari

7

Metformin HCl 50

mg/kg bb

6 27.8117 1.1852

0

.48386 26.567

9

29.0555 26.52 29.42

Suspensi CMC-Na

1%

5 5.0720 4.6292

0

2.0702

4

-.6759 10.8199 1.70 12.05

Crude Ekstrak

Etanol 200 mg/kg

bb

6 49.0400 5.5708

6

2.2742

9

43.193

7

54.8863 39.52 55.02

Ekstrak n-Heksan

200 mg/kg bb

6 31.7817 1.9377

5

.79108 29.748

1

33.8152 29.46 34.66

Ekstrak Etilasetat

200 mg/kg bb

6 43.7667 2.6381

7

1.0770

3

40.998

1

46.5353 39.73 46.50

Ekstrak Etanol 200

mg/kg bb

6 27.6800 4.0074

0

1.6360

2

23.474

5

31.8855 22.13 31.69

Total 35 31.5954 14.133

87

2.3890

6

26.740

3

36.4506 1.70 55.02

Hari

10

Metformin HCl 50

mg/kg bb

6 51.7367 .85047 .34720 50.844

1

52.6292 50.68 53.06

Suspensi CMC-Na

1%

6 4.4467 5.1204

4

2.0904

1

-.9269 9.8202 -.50 13.64

Crude Ekstrak

Etanol 200 mg/kg

bb

6 60.3117 3.2079

1

1.3096

2

56.945

2

63.6782 56.49 64.07

Ekstrak n-Heksan

200 mg/kg bb

6 44.4733 2.0699

2

.84504 42.301

1

46.6456 41.42 46.64

Ekstrak Etilasetat

200 mg/kg bb

6 53.9567 2.6159

3

1.0679

5

51.211

4

56.7019 51.57 57.70

Ekstrak Etanol 200

mg/kg bb

6 54.0567 3.1816

9

1.2989

2

50.717

7

57.3956 50.54 59.06

Total 36 44.8303 19.133

32

3.1888

9

38.356

5

51.3041 -.50 64.07


139

Levene Statistic df1 df2 Sig.

Hari 1 . 5 . .

Hari 3 2.230 5 30 .077

Hari 5 2.105 5 30 .092

Hari 7 3.195 5 29 .020

Hari 10 2.029 5 30 .103

ANOVA

Sum of Squares df

Mean

Square F Sig.

Hari 1 Between Groups .000 5 .000 . .

Within Groups .000 30 .000

Total .000 35

Hari 3 Between Groups 337.437 5 67.487 7.908 .000

Within Groups 256.015 30 8.534

Total 593.452 35



Total 2176.195 35


Within Groups 381.784 29 13.165

Total 6792.051 34



Total 12812.932 35

Test of Homogeneity of Variances


140

Lampiran 15 (lanjutan)

Post Hoc Tests

Homogeneous Subsets

Hari 3

Duncana

Perlakuan N


1 2 3 4

Suspensi CMC-Na 1% 6 2.4500

Ekstrak Etanol 200 mg/kg bb 6 5.1817 5.1817

Ekstrak Etilasetat 200 mg/kg bb 6 5.2483 5.2483

Metformin HCl 50 mg/kg bb 6 7.6067 7.6067

Ekstrak n-Heksan 200 mg/kg bb 6 9.7133 9.7133

Crude Ekstrak Etanol 200 mg/kg bb 6 11.6300

Sig. .127 .184 .221 .265



Hari 5

Duncana

Perlakuan N


1 2 3 4


Metformin HCl 50 mg/kg bb 6 14.1467

Ekstrak Etilasetat 200 mg/kg bb 6 16.7017

Ekstrak Etanol 200 mg/kg bb 6 16.8717

Ekstrak n-Heksan 200 mg/kg bb 6 21.5633


Sig. 1.000 .153 1.000 1.000




141

Lanjutan tabel Post Hoc Test Homogeneous

Subsets

Hari 7

Duncana,,b

Perlakuan N


1 2 3 4







Sig. 1.000 .078 1.000 1.000

Hari 10

Duncana

Perlakuan N


1 2 3 4







Sig. 1.000 1.000 .234 1.000





b. The group sizes are unequal. The harmonic mean of the group sizes is used. Type I

error levels are noguaranteed.


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