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Physalaemus petersi Geographic variation in Male Calls and Female Preferences. Biogeographic Hypotheses for Diversification in Amazonia. Methods. 2,400 bp of 12S and 16S mtDNA. 48 Physalaemus petersi from 18 sites. Outgroups: - P. pustulosus (2) - P. pustulatus (1) - PowerPoint PPT Presentation
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Physalaemus petersiGeographic variation in Male Calls and Female Preferences
Biogeographic Hypotheses for Diversification in Amazonia
Methods
• 48 Physalaemus petersi from 18 sites
• 2,400 bp of 12S and 16S mtDNA
• Outgroups: - P. pustulosus (2) - P. pustulatus (1) - P. coloradorum (1)
Predictions of Biogeographic Hypotheses
Results
Partial Mantel tests indicate that hypothesized refugia still significant after removing effects of geographic distance
Results
* = P < 0.05** = P < 0.01
Test statistic
CladeTajima’s D Mismatch
dist (SSD)Fu’s Fs FLUCTUATE
(g)Northern(e EC, ne PE)
-0.255 0.012 -9.834** 5.360
Southern(w BR, se PE,ne BO)
-1.074 0.036 -4.059* -33.183
Tests of Population Expansion
North-South Break in Western AmazoniaPhysalaemus petersi; current study
Phaeothlypis wood-warblers; Lovette 2004
Dendrobates spp; Symula et al. (2003)
Anolis nitens; Glor et al. 2001
Carollia castanea; Hoffman & Baker 2003
Photo by Arthur Grosset
Photo from Batkey (http://katydid.uni-graz.at/batkey/about.asp)
ResultsLarge Divergence in Male Calls and Female Preferences between Populations that have Diverged Recently
Future directions
Phylogeography1. Additional sites and individuals (central and eastern Brazil)
2. Additional analyses: Parametric bootstrapping
3. Additional loci: Microsatellites
Call variation1. Finish analysis of calls (12 sites)
2. Examine relationship between call divergence and genetic divergence among populations (9 sites with calls & tissues)
Acknowledgements
Collaborators- Ariadne Angulo- Kathryn Boul- Janalee Caldwell- David Cannatella- Luis Coloma- Ignacio De la Riva- José Padial- Santiago Ron- Michael Ryan
Field work- Samael Padilla- Claudia Torres Gastello
Lab work- Brian Caudle - Colin Peden
Analysis advice- Ximena Bernal- Meredith Mahoney- Derrick Zwickl
Funding- NSF IRCEB program