PerkinElmer chemagen Technologie GmbH - APHL … chemagen Technologie GmbH Alex B. Lopez, ... Any instance where Nucleic Acid Extraction is needed ... Easy automation possible Nucleic

1 1 © 2009 PerkinElmer © 2009 PerkinElmer © 2009 PerkinElmer © 2014 PerkinElmer

PerkinElmer chemagen Technologie GmbH

Alex B. Lopez, PhD MBA

June 2, 2014

Nucleic Acid Purification:

A New Perspective

2 2

Who is chemagen?

What can we do for Public Health Laboratories?

chemagen

Synergy within PKI

Core Competence (M-PVA Beads)

Chemistry and Automation

Summary

Human Genetics

Overview



Who is chemagen?

4 4

The Company

Arnold-Sommerfeld-Ring 2

52499 Baesweiler, Germany

Founded

December 1997

Founded

July 2009

February 2011: PerkinElmer, Inc. acquired chemagen

5 5

Any instance where Nucleic Acid Extraction is needed

Pathogen testing from clinical samples

Bacterial and Viral DNA/RNA isolation

Genomic DNA and Total RNA isolation

Waterborne pathogen testing

Extraction of pathogen DNA/RNA from filters

Food Testing

Extraction of DNA/RNA from plants/food/livestock

Together with our Life Sciences and Technology Division

An entire workflow of nucleic acid extraction, quantification, NGS, PCR and WGA can

be completely provided to Public Health Laboratories

Diagnostics Business (Newborn screening, Laboratory Services)

Human Genetics

Interest for Public Health Laboratories

6 6

Routinely Detected Pathogens

HAV, HBV, HCV, HGV, HIV, pB19, CMV, VZV, HPV, HSV (I + II ), EBV

Noroviruses, Influenza, Adenoviruses

Chlamydia, MRSA, FSME, EHEC, Legionella, Listeria, Borrelia, N. Gonorrhoeae,

Ureoplasma, Mycoplasma, Chlostridium

Cryptosporidium and Giardia

From sources

Human body fluids/tissue

Food and livestock

Water

Plants

Human Genetics




Core Competence: M-PVA

8 8 M-PVA Magnetic Beads

Core Competence

Magnetic Polymer Particles “Magnetic Beads“

based on Polyvinyl Alcohol (PVA)

Electron microscopic image

~ 1 µm

Superparamagnetic nanometer sized

magnetite particles encapsulated in a

matrix of crosslinked PVA.

9 9

High magnetite content

enables fast magnetic separation, even from large volumes

Specific carboxylated bead surface with high affinity to nucleic acids

resulting in high quantitative yields of NA

Hydrophilic bead surface with non-measurable binding properties for proteins

resulting in very pure DNA

DNA can easily be eluted at room temperature

(in contrast to e.g. silica particles)

Beads can be dried without affecting the bound DNA or RNA (high purities)

Allows the processing of inhomogeneous/compromised sample material

(leading e.g. to clogging of columns)

Ideal for automation

(no vacuum station or centrifuges necessary)

Key Features

M-PVA Magnetic Bead based DNA/RNA Isolation

MAGNETIC BEADS

ARE IDEAL FOR

AUTOMATION



Chemistry and Automation

11 11

Sample Source

Human Blood

Buffy Coats

Amniotic Fluid

Tissue /

FFPE Tissue

Buccal Swabs/

Saliva

Serum/

Plasma

Kits for Nucleic Acid Isolation

Kit Portfolio

Animal Blood

Plant

Food Bacteria

Cells

PCR Product

Purification

Sequencing Reaction

Cleanup

Human Veterinary

Plant Culture

Amplification Products

To isolate

Pathogen DNA/RNA

Genomic DNA

Total RNA

HUGE VARIETY OF

MAGNETIC BEAD

BASED KITS

ccf DNA

12 12

Highest Sensitivity

chemagic Technology Guarantees Best Virus Sensitivities

Virus Detection

Limits [IU/ml]

HAV 2.61

HBV 0,72

HCV 5,02

HIV 3,32

B19V 4,63

Data kindly provided by

1DRK-Blutspendedienst West gGmbH, Zentrallabor Hagen;

2DRK-Blutspendedienst Sachsen gGmbH, Institut für Transfusionsmedizin Plauen;

3DRK-Blutspendedienst Hessen gGmbH, Institut für Transfusionsmedizin Frankfurt.

chemagic Viral DNA/RNA Kit special

chemagic MSM I

9.6 ml plasma mini

pool samples

ca. 60 min

In house PCR Kits

.

13 13

Principle of Manual Nucleic Acid Isolation with Magnetic Beads

Advantages due to this process

Different kinds of sample material can be used

Easy automation possible

Nucleic Acid Isolation

Lysis Binding Washing Elution

Chemically driven release of

NA depending on the

sample material

Binding of free NA

by Magnetic Beads

Removal of non specificly

bound compounds

Release of pure, ready

to use NA from the

Magnetic Beads

14 14

The chemagic separation and resuspension technology consists of

a magnet that can be switched on and off

metal rods that can be magnetized for efficient collection of particles

a stirring motor to which the metal rods are connected enabling the efficient mixing of particles when the motor is switched

on and the electro magnet is switched off

The chemagic Separation Technology is realized in the

chemagic Magnetic Separation Module I

chemagic Prepito

JANUS chemagic Workstation

Switchable magnet and stirring metal rods

Magnet off

Rotation on

Metal rod covered

with a disposable tip

mounted to a motor;

magnetizable through

an external magnet

Magnet on

chemagic Separation Technology

TRANSFER OF MAGNETIC BEADS

NOT LIQUIDS

15 15 Switchable magnet and stirring metal rods

Automated chemagic Separation Technology

TRANSFER OF MAGNETIC BEADS

NOT LIQUIDS

Efficient collection of particles:

The external magnet generates an electric field

which is strongest at the top of the metal rods

Efficient mixing of particles:

Rotating needles guarantee thorough

resuspension of the particles

Magnet off

Rotation on Magnet on

Metal rod covered with a disposable tip

mounted to a motor;

magnetizable through an external magnet

16 16

The chemagic MSM I – use of an electro magnet to magnetize rods

chemagic Magnetic Separation Module I

Electro magnet

Strongest magnetic field at the top of the rods

The electro magnet consists

of an aluminium coil in a cover.

When the power is switched on,

an electric field (black line) is

generated

The electric field induces a

magnetic field (red lines)

The magnetic field lines are

conducted through metal rods.

The strongest magnetic field

at the top of the rods of the

chemagic Rod Head.

Nearly no magnetic field is

at their sides

17 17

The main parts of the chemagic MSM I

Electro magnet

circular unit with a

hole in the center

chemagic Rod Head

Consisting of metal rods and gearbox

with motor. Mounted to a linear motion

system ensuring its movement up and

down THROUGH the hole in the electro

magnet

X-Axis

Linear motion axis on which all required

process solutions are placed in appropriate

reaction vessels

18 18

chemagic Accessories

Modular setup guarantees maximal flexibility

chemagic Dispenser

Simultaneous filling of up to 8 different buffers

with different buffer volumes

Available for all three head formats

Fully integrated in the chemagic MSM I software

Reduces hands-on time

Waste of buffer is minimized

Based on membrane pump technology

chemagic

96 Rod Head

chemagic

24 Rod Head

chemagic

12 Rod Head

Processable

Sample Volume 10 µl – 400 µl 200 µl – 4 ml 1 ml – 10 ml

Samples per Run 1 – 96 1 – 24 1 – 12

chemagic Rod Heads

chemagic QA Software

Barcode reading/Sample tracking

LIMS compatible log files

User management

Easy navigation and set up

Pre-installed protocols

Tracking the progress of a running protocol

19 19

Schematic overview of the isolation process with the chemagic MSM I

chemagic Separation Technology

chemagic MSM I, equipped with the chemagic 96 Rod Head,

for low volume applications, from up to 400 µl sample volume

20 20

Chess board test with high titer and negative Norovirus samples

chemagic Viral DNA/RNA Kit special

chemagic Technology Guarantees no Cross Contamination

Real Time PCR

Positive und negative Norovirus samples were

processed with the chemagic MSM I

in one run.

In the subsequent Real-Time PCR

no cross contamination occurred.

TRANSFER

OF BEADS NOT LIQUIDS

21 21

Features at a Glance

Huge volume flexibility: one instrument for all sample volumes

(10 µl – 10 ml)

12 large volume samples in 45 – 60 min

24 medium volume samples in 35 – 60 min

96 low volume samples in 15 – 60 min

Huge kit portfolio

Enhanced success rates for downstream applications

(no drop outs)

Optional buffer dispensing and sample tracking

(bar code reading)

No cross contamination

Highest consistency

Automation and chemistry from the same company

Easy to connect to Liquid Handling platforms

The chemagic Magnetic Separation Module I

http://www.chemagen.com/chemagic-concept.html

Metal rod covered with a

disposable tip

mounted to a motor;

magnetizable through an

external magnet

22 22

chemagen Technology adopted to Liquid Handling Instruments

chemagic MSM I + LH chemagic Kits adopted to LH Instruments

From semiautomated workflow to fully automated solution

Janus/chemagic MSM I

Linear arrangement of a

chemagic MSM I with a PKI LH

Angulate arrangement of a

chemagic MSM I with a PKI LH

chemagic MSM I +

HAMILTON Star

chemagic MSM I +

BECKMAN COULTER

Biomek NX

chemagic MSM I +

TECAN Freedom Evo 150

chemagic MSM I +

TECAN Freedom Evo 150

with Bigneat housing

chemagic Kits LH +

PerkinElmer

JANUS Mini MDT

chemagic Kits LH +

PerkinElmer

JANUS expanded

chemagic Kits LH +

BECKMAN COULTER

Biomek® FX Robotic

Workstation

AUTOMATION

SOLUTIONS FOR

EVERY DEMAND

23 23

JANUS chemagic Workstation

Key Features and Benefits

Independent liquid handling and extraction scheduling:

Primary sample transfer, normalization and PCR setup applications

Up to 192 samples per run with 96-rod head

Up to 48 samples per run with 24-rod head

Sample volume range from 10 µl - 4 ml

Standardized pre-programed protocols for reproducible,

high throughput extraction of DNA in blood or saliva

Intuitive graphical user interface

Ideal solution for the unique requirements needed in

PCR/qPCR,

Genotyping

Genome wide analysis

24 24

JANUS chemagic Workstation Plus - Configuration

All standard features of the standard Workstation, with the following changes:

8-tip VariSpan Liquid Handling Workstation

Gripper Arm

Automated Tube Barcode Reader

Enclosure with Table

Optional integration of LabChip DS for

UV/Vis quantitation and sample normalization

Completion of genomic analysis workflows with LabChip® Microfluidic Technology

Genomic DNA assay is used to generate accurate size, concentration,

and quality assessment of intact, full length DNA

Additional DNA and RNA assays are available

LabChip DS analysis of a 96-well plate of

gDNA isolated from different blood volumes

using the JANUS chemagic Workstation.

LabChip GX analysis of gDNA isolated from 2 ml and 300 µl blood sample

volumes (Figures 1A and 1B) using the JANUS chemagic Workstation.

Virtual gel images created within the LabChip software illustrate intact

gDNA post extraction. n=24. Sample 1 is a blank.

25 25

chemagic 360 - Compact High Volume Nucleic Acid Isolation

Key Features and Benefits

Revolutionary compact benchtop design

800 x 650 x 900 mm (L/WH)

Sample Volumes from 10 µl - 10 ml

High throughput

Facilitating reliable and approved

chemagic Separation Technology, ensuring

No cross contamination

Smooth and efficient resuspension technology

Convenient and improved sample management

User-friendly modular system

Integrates with LH platforms

Quality Assurance through

Bar code reading for sample tracking

Ease of use chemagic QA Software

Unique Selling Proposition

The only compact benchtop,

high volume, high throughput system on the market

chemagic 360, equipped with chemagic 12 Rod Head

and chemagic Dispenser 360



Summary

27 27

Unique M-PVA magnetic beads and chemistry for nucleic acid isolation

MSM I technology is designed to specifically work with M-PVA beads for a premium

nucleic acid purification

We can offer Public Health Laboratories means for pathogen detection, human and

animal genetics and food testing through nucleic acid purification

PerkinElmer is able to provide a full-range of solutions from nucleic acid isolation to

detection to analysis

Human Genetics


28 28 Key Customers Worldwide

Selection of Worldwide chemagic MSM I Customers

29 29

chemagen Technology

Thank you for your attention!


Arnold-Sommerfeld-Ring 2

D-52499 Baesweiler

Documents

PerkinElmer chemagen Technologie GmbH - APHL … chemagen Technologie GmbH Alex B. Lopez, ... Any instance where Nucleic Acid Extraction is needed ... Easy automation possible Nucleic