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JNC CORPORATION

pCL41 Cat. No. P-008 Gene/Insert name: Wild-type Clytin I (C. gregaria) Vector backbone: pBluescript SK Vector type: E. coli Backbone size w/o insert (bp): 2,958 Bacterial resistance: Ampicillin Growth strain: JM83 Growth temperature (oC): 37 Growth instructions: pCL41 is resistant to ampicillin (50 µg/mL) High or low copy: High copy Vector map: pCL41 Coding sequence: Nucleotide sequence & Amino acid sequence Plasmid sequence: pCL41 (3,726 bp) Restriction enzyme list: Restriction enzyme sites of pCL41 GenBank Accession No.: L13247 Size: 10 µg Constituents: 10 mM Tris-HCl (pH 7.6)－1 mM EDTA Terms and Licenses: MTA

Laboratory Reagent For Research Use Only

Feature for pCL41:Sourse 1-331 1-230

Residue 1-331 1-230 380-973 1-594

CommentspBluescript SK backbone lac promoter/operator

1,094-3,726 326-2,958 pBluescript SK backbone

3,553 2,785 ori: Origin of replication1,925-2,785 1,157-2,017 Ampr: Ampicillin resistance gene

JNC Corporation, Yokohama Research Center

Name: pCL41Insert: Wild-type Clytin I cDNA Vector: pBluescript SK

Ref.1)

M A D T - - - F V P ***DNA fragment:・

・

JNC CORPORATION

Cat. No. P-008

1,356-1,662 588-894 f1(+) origin

Wild-type Clytin I cDNA

HindIIIHindIII

HindIII

EcoRI

EcoRI

EcoRI

NotI

NotI

NotI

XhoI

PstI

f1(+) origin

Ampr

pCL41(3,726 bp)

orilac promoter/operator

Ca2+-Binding Photoprotein, Clytin I

ATG-GCT-GAC-ACT-.......-TTT-GTT-CCT-TAA

Clytin I amino acid seq. & cDNA seq.: GenBank Accession No. L13247 Inouye, S. and Tsuji, F.I. FEBS Lett. (1993) 315: 343-346.

Wild-type Clytin I ORF

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JNC CORPORATION

Cat. No. P-008 Gene coding region (ORF: Clytin I)

Nucleotide sequence GAATTCGCGGCCGCATCAACTTTTGCAACTCAAAGCAAATTTCAAAACTTCAACATGGCTGACACTGCATCAAAATACGCCGTCAAACTCAGACCCAACTTCGACAACCCAAAATGGGTCAACAGACACAAATTTATGTTCAACTTTTTGGACATTAACGGCGACGGAAAAATCACTTTGGATGAAATCGTCTCCAAAGCTTCGGATGACATTTGCGCCAAACTTGGAGCAACACCAGAACAGACCAAACGTCACCAGGATGCTGTCGAAGCTTTCTTCAAAAAGATTGGTATGGATTATGGTAAAGAAGTCGAATTCCCAGCTTTTGTTGATGGATGGAAAGAACTGGCCAATTATGACTTGAAACTTTGGTCTCAAAACAAGAAATCTTTGATCCGCGACTGGGGAGAAGCTGTTTTCGACATTTTTGACAAAGACGGAAGTGGCTCAATCAGTTTGGACGAATGGAAGGCTTATGGACGAATCTCTGGAATCTGCTCATCAGACGAAGACGCCGAAAAGACCTTCAAACATTGCGATTTGGACAACAGTGGCAAACTTGATGTTGATGAGATGACCAGACAACATTTGGGATTCTGGTACACCTTGGACCCCAACGCTGATGGTCTTTACGGCAATTTTGTTCCTTAAACATCGAAACAAAAGCCCAAAAGAAGTTTTGGAAGAATTATTTGATACTATCATTTGTTACTATTTCGTAACATGCTATATTTTGTAACATGCTATATTTTATAATTTTGCGGCCGCGAATTC

Amino acid sequence EFAAASTFATQSKFQNFNMADTASKYAVKLRPNFDNPKWVNRHKFMFNFLDINGDGKITLDEIVSKASDDICAKLGATPEQTKRHQDAVEAFFKKIGMDYGKEVEFPAFVDGWKELANYDLKLWSQNKKSLIRDWGEAVFDIFDKDGSGSISLDEWKAYGRISGICSSDEDAEKTFKHCDLDNSGKLDVDEMTRQHLGFWYTLDPNADGLYGNFVP*

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JNC CORPORATION

Cat. No. P-008pCL41 (3,726 bp)

GCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGAAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCCACCGCGGTGGCGGCCGCTCTAGAACTAGTGGATCCCCCGGGCTGCAGGAATTCGCGGCCGCATCAACTTTTGCAACTCAAAGCAAATTTCAAAACTTCAACATGGCTGACACTGCATCAAAATACGCCGTCAAACTCAGACCCAACTTCGACAACCCAAAATGGGTCAACAGACACAAATTTATGTTCAACTTTTTGGACATTAACGGCGACGGAAAAATCACTTTGGATGAAATCGTCTCCAAAGCTTCGGATGACATTTGCGCCAAACTTGGAGCAACACCAGAACAGACCAAACGTCACCAGGATGCTGTCGAAGCTTTCTTCAAAAAGATTGGTATGGATTATGGTAAAGAAGTCGAATTCCCAGCTTTTGTTGATGGATGGAAAGAACTGGCCAATTATGACTTGAAACTTTGGTCTCAAAACAAGAAATCTTTGATCCGCGACTGGGGAGAAGCTGTTTTCGACATTTTTGACAAAGACGGAAGTGGCTCAATCAGTTTGGACGAATGGAAGGCTTATGGACGAATCTCTGGAATCTGCTCATCAGACGAAGACGCCGAAAAGACCTTCAAACATTGCGATTTGGACAACAGTGGCAAACTTGATGTTGATGAGATGACCAGACAACATTTGGGATTCTGGTACACCTTGGACCCCAACGCTGATGGTCTTTACGGCAATTTTGTTCCTTAAACATCGAAACAAAAGCCCAAAAGAAGTTTTGGAAGAATTATTTGATACTATCATTTGTTACTATTTCGTAACATGCTATATTTTGTAACATGCTATATTTTATAATTTTGCGGCCGCGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAGGGGGGGCCCGGTACCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGA

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JNC CORPORATION

Residue Sourse Comments1-331 1-331 pBluescript SK backbone 1-230 1-230 lac promoter/operator 380-973 1-594 Wild-type Clytin I ORF 1,094-3,726 326-2,958 pBluescript SK backbone 1,356-1,662 588-894 f1(+) origin 3,553 2,785 ori: Origin of replication 1,925-2,785 1,157-2,017 Ampr: Ampicillin resistance gene

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JNC CORPORATION

Cat. No. P-008Restriction enzyme sites of pCL41

Enzyme Name Sequence Count Cutting Positions AccI GT!MKAC 1 1123ApaI GGGCC!C 1 1141Asp718I G!GTACC 1 1143BamHI G!GATCC 1 309BclI T!GATCA 0 -BglII A!GATCT 0 -EcoRI G!AATTC 3 327, 639, 1095 EcoRV GAT!ATC 1 1103HincII GTY!RAC 2 446, 1124HindIII A!AGCTT 3 523, 595, 1107 KpnI GGTAC!C 1 1147MluI A!CGCGT 0 -NcoI C!CATGG 0 -NdeI CA!TATG 0 -NheI G!CTAGC 0 -NotI GC!GGCCGC 3 290, 334, 1088 PstI CTGCA!G 1 325SacI GAGCT!C 1 277SalI G!TCGAC 1 1122ScaI AGT!ACT 1 2232SmaI CCC!GGG 1 317StuI AGG!CCT 0 -XbaI T!CTAGA 1 297XhoI C!TCGAG 1 1128

JNC Corporation, Yokohama Research Center

5-1 Okawa, Kanazawa-ku, Yokohama, Japan 236-8605Tel: 045-786-5501 Fax: 045-786-5511 E-mail: jphoton@jnc-corp.co.jp

Contact usSupplier

Shin Otemachi Bldg. 9F 2-2-1 Otemachi, Chiyoda-ku, Tokyo 100-8105 URL http://www.jnc-corp.co.jp

JNC CORPORATION

SAFETY DATA SHEETAccording to JIS Z 7253:2012

Creation Date 01-Aug-2016

Revision Date

Section 1: PRODUCT AND COMPANY IDENTIFICATION

Product name pCL41Product code P-008Recommended uses and restrictions on use

For research purposes

Manufacturer

Supplier

Emergency telephone number

JNC CORPORATION,Yokohama research Center5-1, Okawa, Kanazawa-ku, Yokohama 236-8605, Japan Phone: +81-45-786-5501Fax: +81-45-786-5511JNC CORPORATIONShin Otemachi Bldg., 2-2-1 Otemachi, Chiyoda-ku, Tokyo 100-8105Phone: +81-3-3243-6330Fax: +81-3-3243-6363+81-45-786-5501

Section 2: HAZARDS IDENTIFICATION

GHS classificationClassification of the substance or mixture

･ Not a hazardous substance or mixture according to the Globally Harmonized System (GHS)

Pictograms

Signal word･ none

Hazard statements･ Not a hazardous substance or mixture according to the Globally Harmonized System (GHS)

Precautionary statements-(Prevention)

• Not applicablePrecautionary statements-(Response)

• Not applicablePrecautionary statements-(Storage)• Not applicable

Precautionary statements-(Disposal)

• Not applicable

Others Other hazards

･ Not available

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Section 3: COMPOSITION/INFORMATION ON INGREDIENTS

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Mixture

CPS-R-0037Y pCL41

･ none

Chemical Name Weight-% Molecular weight EC No. ISHL No. CAS No.EDTA <0.1% 292.24 200-449-4 N/A 60-00-4

Creation Date 01-Aug-2016 _____________________________________________________________________________________________

Section 4: FIRST AID MEASURES

InhalationRemove to fresh air. If symptoms persist, call a physician.

Skin contactWash off immediately with soap and plenty of water. If symptoms persist, call a physician.

Eye contactIF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. Immediate medical attention is required.

IngestionRinse mouth. Never give anything by mouth to an unconscious person. Call a physician or poison control center immediately.Do not induce vomiting without medical advice.

Protection of first-aidersUse personal protective equipment as required.

Section 5: FIRE FIGHTING MEASURES

Suitable extinguishing mediaWater spray (fog), carbon dioxide (CO2), Foam, Extinguishing powder, Sand

Unsuitable extinguishing mediaNo information available

Special extinguishing methodNo information available

Specific hazards arising from the chemical productThermal decomposition can lead to release of irritating and toxic gases and vapors.

Protection of fire-fightersUse personal protective equipment as required. Firefighters should wear self-contained breathing apparatus and full firefightingturnout gear.

Section 6: ACCIDENTAL RELEASE MEASURES

Personal precautions, protective equipment and emergency proceduresFor indoor, provide adequate ventilation process until the end of working. Deny unnecessary entry other than the peopleinvolved by, for example, using a rope. While working, wear appropriate protective equipments to avoid adhering it on skin, orinhaling the gas. Work from windward, and retract the people downwind.

Environmental precautionsTo be careful not discharged to the environment without being properly handled waste water contaminated.

Methods and materials for contaminent and methods and materials for cleaning up

_____________________________________________________________________________________________

Do not touch spilled material without suitable protection(See section 8). After material is completely picked up, wash the spill site with soap and water and ventilate the area. Put all wastes in a plastic bag for disposal and seal it tightly. Remove, clean, or dispose of contaminated clothing.

Recoverly, neutralization

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No information availableSecondary disaster prevention measures

Clean contaminated objects and areas thoroughly observing environmental regulations.

CPS-R-0037Y pCL41

Section 7: HANDLING AND STORAGE

Handling Technical measures

Avoid contact with strong oxidizing agents. Use with local exhaust ventilation.Precautions

Creation Date 01-Aug-2016 _____________________________________________________________________________________________

Do not rough handling containers, such as upsetting, falling, giving a shock, and dragging. Prevent leakage, overflow, andscattering. Not to generate steam and dust in vain. Seal the container after use. After handling, wash hands and face, and thengargle. In places other than those specified, should not be smoking or eating and drinking. Should not be brought contaminatedprotective equipment and gloves to rest stops. Deny unnecessary entry of non-emergency personnel to the handling area.

Safety handling precautionsAvoid contact with skin, eyes or clothing. Use personal protective equipment as required.

Storage Safe storage conditions

Storage conditions Container protected from light, and store tightly closed in freezer (-20°C).

Safe packaging materialIncompatible substances

Glass or Plastic containerStrong oxidizing agents

Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION

Engineering controlsIn case of indoor workplace, seal the source or use a local exhaust system. Provide the safety shower facility, and hand- andeye-wash facility. And display their position clearly.

Exposure limits

This product, as supplied, does not contain any hazardous materials with occupational exposure limits established by the region specific regulatory bodies.

Personal protective equipmentDust maskProtection glovesprotective eyeglasses or chemical safety goggles

Respiratory protection Hand protectionEye protectionSkin and body protection Long-sleeved work clothes

General hygiene considerationsHandle in accordance with good industrial hygiene and safety practice.

Section 9: PHYSICAL AND CHEMICAL PROPERTIES

Form

AppearanceColorless

Odor No data availablepH No data availableMelting point/freezing point No data availableBoiling point, initial boiling point and boiling range No data availableFlash point No data availableEvaporation rate: No data availableFlammability (solid, gas): No data availableUpper/lower flammability orexplosive limits

Color

Lower：：：：Vapour pressure

_____________________________________________________________________________________________

Vapour densitySpecific Gravity / Relative density

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Solubilitiesn-Octanol/water partition coefficient:(log Pow)Auto-ignition temperature:

CPS-R-0037Y pCL41

Decomposition temperature:

No data availableNo data available No data available No data available No data available Water: soluble No data available No data available No data available

No data available

Viscosity (coefficient of viscosity)Dynamic viscosity

No data availableNo data available

Creation Date 01-Aug-2016 _____________________________________________________________________________________________

Section 10: STABILITY AND REACTIVITY

Stability

Stability Stable under the recommendated strage conditionReactivity No data available

Hazardous reactionsNone under normal processing

Conditions to avoidExtremes of temperature and direct sunlight

Incompatible materialsStrong oxidizing agents

Hazardous decomposition productsCarbon monooxide (CO), carbon dioxide (CO2), Nitrogen oxides (NOx)

Section 11: TOXICOLOGICAL INFORMATION

Acute toxicity No data available

Skin irritation/corrosion No data availableSerious eye damage/ irritation No data availableRespiratory or skin sensitization No data availableReproductive cell mutagenicity No data availableCarcinogenicity No data available

Reproductive toxicity No data availableSTOT-single exposure No data availableSTOT-repeated exposure No data availableAspiration hazard No data available

Section 12: ECOLOGICAL INFORMATION

Ecotoxicity No information available

Other data No data available

Persistence and degradability No information availableBioaccumulative potential No information availableMobility in soil No information availableHazard to the ozone layer No information available

_____________________________________________________________________________________________

Section 13: DISPOSAL CONSIDERATIONS

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Waste from residuesDisposal should be in accordance with applicable regional, national and local laws and regulations.

CPS-R-0037Y

Contaminated container and contaminated packaging

pCL41

Disposal should be in accordance with applicable regional, national and local laws and regulations.

Creation Date 01-Aug-2016 _____________________________________________________________________________________________

Section 14: TRANSPORT INFORMATION

ADR/RID Not regulatedUN number -Proper shipping name:UN classficationSubsidiary hazard classPacking groupMarine pollutant Not applicable

IMDG Not regulatedUN number -Proper shipping name:UN classficationSubsidiary hazard classPacking groupMarine pollutant (Sea) Not applicableTransport in bulk according toAnnex II of MARPOL 73/78 andthe IBC Code

No information available

IATA Not regulatedUN number -Proper shipping name:UN classficationSubsidiary hazard classPacking groupEnvironmentally HazardousSubstance

Not applicable

Section 15: REGULATORY INFORMATION

International Inventories EINECS/ELINCS -TSCA -

Japanese regulations Fire Service Act Not applicablePoisonous and DeleteriousSubstances Control Law

Not applicable

Industrial Safety and Health Act Not applicableAct on the Evaluation ofChemical Substances andRegulation of Their Manufacture,etc

Not applicable

Regulations for the carriage andstorage of dangerous goods inship

Not applicable

Civil Aeronautics Law Not applicable

_____________________________________________________________________________________________

Pollutant Release and TransferRegister Law

Not applicable

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Export Trade Control Order Not applicable

Section 16: OTHER INFORMATION

CPS-R-0037Y pCL41

Literature and references

Creation Date 01-Aug-2016 _____________________________________________________________________________________________

DisclaimerThe information provided in this Safety Data Sheet is correct to the best of our knowledge, information and belief at the date of itspublication. The information given is designed only as a guidance for safe handling, and is not to be considered a warranty orquality specification. The information relates only to the specific material designated and may not be valid for such material used incombination with any other materials or in any process, unless specified in the text.GHS Classification is acoording to JIS Z7252(2010). *JIS: Japanese Industrial Standards

_____________________________________________________________________________________________

End of Safety Data Sheet

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