P I O N E E R I N G D I A G N O S T I C S

Pathogen Detection and Quality Indicators

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Raw MaterialEnvironmental SamplesSemi-finished Products

Finished ProductsMicrobial detection

LEADER IN INDUSTRIAL MICROBIOLOGYFOOD INDUSTRY APPLICATIONS

Media Preparation

Sample Preparation

Enumeration Identification

The food microbiology pathway: from sample to decision

Tempo®

Masterclave®

APS ONE®

Dilumat® Smasher® Labguard®

VIDAS®

VITEK® MS

VITEK® 2 Compact

API

IT Solutions

ALOA Plate

D-Count®

Gene-Up*

* Available soon

Pathogen Detection

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GENE-UP®

GENE-UP®

Real-Time PCR Pathogen Detection System• PCR-based molecular diagnostic system for the detection of

bacteria and viruses in food, raw materials and the production environment

• 6-Channel Multiplex qPCR Thermocycler with Routine Software and Open Software

• Combination of BioFire’s know-how in molecular biology and bioMérieux’s expertise in microbiology

• Added value: detection of food pathogen bacteria and viruses, precision of PCR molecular biology, improved lab productivity and limited risk of inter-sample contamination

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GENE-UP REAGENTS

• Generic 5 min. Mechanical Lysis• 192 Ready-to-Use lysis tubes

per kit• Room Temperature Storage• Patented Magic Cap• Red Lysis ensures traceability

• Salmonella, Cronobacter, Listeria spp, Listeria mono, E. coli O157:H7, STEC

• 192 PCR tests per kit• Room Temperature Storage• Internal Control in every test• Blue dye ensures traceability

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Amplification & Detection

SPIN DOWN(10 sec)

ENRICHMENT in X mL BPW Broth

Selective AGAR

ConfirmationSample Prep

Transfer 10µl Lysate intoPCR TUBES

PCR Tubes

Transfer20µL into

LYSIS TUBES(Red Dye)

MECHANICAL LYSIS

(15 min) PCR

GENE-UP METHOD: SIMPLIFIED WORKFLOW WITH THE GENEPROOF ASSAY

Transfer 30µl of

GENEPROOF PCR MIX

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FRET TECHNOLOGY PRINCIPLE

Target

640 nm detection

470 nm light

Internal Amplification

Control

705 nm detection

470 nm light

640 nm detection

705 nm detection

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GENE-UP PROBE-BASED MELT PEAK ANALYSIS

Melt peak analysis is more sensitive than other real time PCR and is

valuable for detecting low level samples.

IncreaseTemperature

Cool PCR reaction

Probes bind DNA

Probes melt off DNA

High Fluorescence

Low Fluorescence

negative derivative

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GENE-UP PROBE-BASED MELT PEAK ANALYSIS

Sample 13 amplified poorly during PCR but was called positive by melt and confirmed by culture

Low level sample + negatives

Amplification Curves

Melting peaks

This is Confidence

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GENE-UP ADVANTAGES

3 Levels of Specificity with FRET Technology Internal Control with every test Rapid, Standardized Workflow for all assay parameters

- <30 minutes sample prep time for 96 tests Ultra Fast and Efficient mechanical lysis procedure Only two enrichment broths used:

- BPW for GENE-UP E. coli O157:H7, STEC, Salmonella spp. and Cronobacter

- LPT Broth for GENE-UP Listeria spp. and L. monocytogenes

Assay Mix & Match Capability Open Platform

- foodborne virus detection, GMO, allergen testing, etc. Remote Access Software Room Temperature reagent storage bioMérieux Service and Support

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GENE-UP: WORLD WIDE VALIDATIONS

Full Suite of AOAC-RI and ISO/AFNOR Validations

Salmonella

E. coli O157:H7

Listeria spp.

L. monocytogenes

EHEC Series

Cronobacter*

Various Matrices and Sample Sizes

*Cronobacter assay offers enrichment harmony with Salmonella assay

P I O N E E R I N G D I A G N O S T I C S