- 1. Second year Nursing1433- 1434 A.H. / 2012- 2013Microbiology
Practical (Course I) By:Hibah A. W. Abusulaiman
2. Who knows these gentlemen?! 3. What about this lady?! 4. She
was afraid of being infected by 5. This is called:Health Associated
Infection (HAI) 6. The major cause of HAI isThe lack of compliance
by health care workers with basic prevention techniques. 7. The
word infection is most likelyrelated to 8. Microbes 9. 4 WH
Questions What are microbes? Where do they live? When do they
survive? Why they are participating other life forms? 10. So the
study of microbes iscalledMicrobiology 11. Why do we study medical
microbiology??? 12. Nine out of ten cells in our bodies are
microbes So, why are we not suffering fromany disease all the time?
13. When microbes can cause a disease?Weak immune system.Pathogenic
microbial invasion.Invasion to a sterile body site. 14. Microbes
IncludeBacteria.Viruses.Parasites.Fungi. 15. Bacteria Unicellular
prokaryotes. Examples: Staphylococcus spp. Vibrio cholera. 16.
Viruses Smallest infectious particle. Example: Rotavirus. 17.
Parasites Eukaryotes, unicellular or multicelluar. Example: Giardia
lambia. 18. Fungi Eukaryotes, in a unicellular or filamentousform.
Example: Trichophyton rubrum 19. Microbes are responsible
aboutinfectionsThus they are responsible about HAI 20. Objectives
Microbiology Lab safety. The preventive measures of infection
forhealthcare teams. 21. Sense we will enter a microbiologylab, we
have to know about Microbiology lab safety 22. Before enteringThe
Microbiology LabBe sure offollowing the safety rules 23. Safety
inMicrobiology Laboratory 24. Aims of following safety rules and
practices To prevent the occurrence of accidentalinfections in the
lab and reduce them if theyoccur. Prevent potentially harmful
microbes fromleaving the lab. 25. Safety in the Microbiology Lab
Wash your hands with soap and water before you begin and after you
finish your work and before leaving the lab. 26. Safety in the
Microbiology Lab Decontaminate your work surface before and after
you work 27. Safety in the Microbiology Lab No bags inside the lab.
28. Safety in the Microbiology Lab Wear only closed-toed shoes in
the laboratory. 29. Safety in the Microbiology Lab Wear lab scrub
and fully- buttoned lab coat. 30. Safety in the Microbiology Lab
Tight your hair. No cosmetics 31. Safety in the Microbiology Lab If
you are: Injured Immunocompromised.. Pregnant Allergic to latix 32.
Safety in the Microbiology Lab Be careful not to burn yourself when
using Bunsen burner. 33. Safety in the Microbiology Lab Keep your
hands away from your mouth and eyes 34. Safety in the Microbiology
Lab Do not eat, drink or chew gum while you are in the lab 35.
Safety in the Microbiology Lab Avoid using electronic devices
Mobiles, MP3players, laptops 36. Safety in the Microbiology Lab
Never mouth pipetting. 37. Safety in the Microbiology Lab Always
keep cultures in a test tube rack whether you are working at your
bench or walking about in the lab. 38. Safety in the Microbiology
Lab Place all contaminated tubes, plates and waste materials in
appropriate receptacles for subsequent sterilization. 39. Safety in
the Microbiology Lab If you spill a chemical on your skin,
immediately wash the affected area. If a chemical or a culture gets
into your eye, immediately wash your eye for 5 min. 40. Safety in
the Microbiology Lab If anything containing viable bacteria
spills,cover the spell or broken glass with a papertowel and a
generous amount of disinfectantto remain on the slip for at least
20 min.before cleaning it up. 41. Safety in the Microbiology Lab
Sweep up pieces of broken glass (afterdisinfection if necessary)
and place them inthe specified container. 42. Safety in the
Microbiology Lab Place sharps, such as syringes and slides, in a
designated sharp containers. 43. Safety in the Microbiology Lab Do
not keep cultures in your lab drawer orlocker when you have
finished with them. Do not remove cultures, reagents, or
othermaterials from the lab unless you havepermission to do so. 44.
Safety in the Microbiology Lab Know the first aid kit location in
your lab. 45. So, do we have to apply suchrules in the hospital?!
Yes, because 46. You have to apply : The Preventive
MeasuresPersonal Procedural Environmental 47. 1. Personal Hand
hygiene. Fingernails, artificial nails and jewelry. Post exposure
prophylaxis. Prevention of ventilator associatedpneumonia. Safe
injection practices. 48. 1. Personal Hand hygiene: (Resident flora
and transient flora) Be sure to wash your hand before andafter the
patient contact Be sure to wash your hand before startinginvasive
procedures Soaps are detergent based products that have mini ifany
antimicrobial activity. Alcohol based hand rubs have activity
against broadspectrum of medically important pathogens. 49. 1.
Personal Fingernails, artificial nails and jewelry: The subungual
area of the fingers harbor high concentrations of bacteria both
before and after handwashing. Wearing at least one ring on the
dominant hand increases the number of microbes contaminating the
hand. 50. 1. Personal Post exposure prophylaxis: TB, HBV, HCV, HIV
and influenza viruses Prevention of ventilator associatedpneumonia:
arises when there is bacterial invasion of thepulmonary parenchyma
in a patient receivingmechanical ventilation Eliminate
contamination of equipment Practice hand hygiene prior to and
following care 51. 1. Personal Safe injection practices: Unsafe
injection practices have been the source ofmany HCV outbreaks. 52.
2. Procedural Sterilization and disinfection. 53. 3. Environmental
House keeping practices for environmentalsurfaces: Environmental
surfaces (e.g., floors) are considered noncritical surfaces
requiring low-level disinfection. When equipment and environmental
surfaces are visibly contaminated with blood, body fluids or other
potentially infectious materials, or are soiled with or used in the
presence of infectious materials, decontamination should be
performed immediately following a procedure. 54. 3. Environmental
Laundry: laundry which has been soiled with blood or
otherpotentially infectious materials . Contaminatedlaundry shall
be handled as little aspossible, placed and transported in labeled
orcolor-coded bags. 55. 3. Environmental Personal protective
equipments: specialized clothing or equipment worn by anemployee
for protection against a hazard Hand hygiene should be performed
immediatelyafter the removal of any personal protectiveequipment.
56. 3. Environmental contaminant, labeling, and disposal
ofbiohazard waste: Biohazardous waste includes liquid or
semi-liquidblood or other potentially infectious
materials;contaminated items that would release blood orinfectious
materials in a liquid or semi-liquid state. All containers should
be labeled or color-coded. 57. To Contact Me Register on FB: Hibahs
microclasses Note: please send your name, departmentand Gp# e-mail:
[email protected] 58. Thank you for paying attentionAny
Questions???
