Table S1. QTL analyses QTL Traita Chromosome QTL position (cM) b Markerc LOD score R2 valued ae ZnT1 RelPR150 1 55.6 F5I14 5.99 12 3.66 ZnT2 RelPR150 3 6.0 ATHCHIB2 14.1 29 5.58 ZnT3 RelPR150 5 51.2 MSAT5.9 6.94 16 4.13

a. Relative variation of the Primary Root length between 150 µM Zn and control condition (RelPR150) b. The position of the QTL is expressed in cM from the 1st marker of the chromosome c. Name of the closest marker, according to the initial mapping approach d. Percentage of variance explained by the QTL e. Additive effect. Positive value indicates that for the three QTLs, the presence of the Sha allele at QTL

decreases root length in the presence of Zn.

Pineau Supp. Table S1

Table S2. List of A. thaliana accessions genotyped at FRD3 and their haplotypes Name Haplotypea AV codeb NASC codec Name Haplotype AV code NASC code

9481B Sha 261AV CS22460 Mh-0 Col 175AV N904 Abd-0 Ita 177AV N932 Mh-1 Col 215AV N1368 Akita Ita 252AV - Mir-0 Col 216AV N1378 Alc-0 Cvi 178AV N1656 Ms-0 Ita 93AV N905 Altaï-2 Cvi 547AV - Mt-0 Ita 94AV N1380 Altai-5 Cvi 548AV - N10 Ita 265AV CS22488 An-1 Ita 96AV N944 N13 Col 266AV CS22491

Anz-1a Col

705AV N14 Col 267AV CS22492 Bay-0 Bay 41AV N954 N15 Ita 268AV CS22493 Bik-1 Col 541AV - N4 Col 260AV CS22482 Bik-7 Cvi 543AV - N6 Ita 262AV CS22484 Bl-1 Ita 42AV N968 N7 Col 263AV CS22485 Bla-1 Ita 76AV N970 N8 Ita 264AV CS22486 Blh-1 Cvi 180AV N1030 Nd-1 Bay 220AV N1636 Br-0 Ita 60AV N994 Neo-3 Sha 539AV - Bur-0 Col 172AV N1028 Néo-6 Sha 540AV - C24 Ita 183AV N906 Niigata Ita 255AV - Can-0 Bay 163AV N1064 No-0 Bay 49AV N3081 Chi-0 Ita 89AV N1072 Ost-0 Col 61AV N1430 Co-4 Bay 174AV N1090 Oy-0 Col 224AV N1436 Col-0 Col 186AV N1092 Pa-1 Ita 50AV N1438 Ct-1 Bay 162AV N1094 Per-1 Ita 100AV N1444 Cvi-0 Cvi 166AV N902 Petergof Ita 226AV N926 Db-1 Col 132AV N1102 Pi-0 Ita 40AV N1454 Dja-1 Cvi 534AV - Pyl-1 Ita 8AV - Dja-5 Cvi 535AV - Qar-8a Col 549AV - Dr-0 Ita 51AV N1114 Rak-2 Ita 228AV N1484 Dra-0 Col 194AV N1116 Ran Col 21AV - Edi-0 Bay 83AV N1122 Ri-0 Ita 160AV N1492 Enkheim-T Ita 197AV N921 Rld-2 Col 229AV N1641 Est-0 Ita 71AV N1148 Rsch-4 Ita 90AV N1494 Fl-1 Col 199AV N1160 Rubezhnoe-1 Cvi 231AV N927 Ge-0 Ita 101AV N1186 Sah-0 Ita 233AV N1500 Gr-3 Col 58AV N1202 Sakata Ita 257AV - Gre-0 Bay 200AV N1210 Sap-0 Bay 234AV N1506 Had-1b Cvi 544AV - Sav-0 Ita 235AV N1514 Hiroshima Sha 254AV - Shahdara Sha 236AV N929 Hodja-Obi-Garm

Cvi 203AV CS922 Sorbo

Ita 238AV CS931

Ishikawa Sha 253AV - Sp-0 Col 53AV N1530 Ita-0 Ita 157AV N1244 St-0 Bay 62AV N1534 Jea Col 25AV - Stw-0 Sha 92AV N1538 Jl-3 Ita 205AV N1252 Sus-1 Cvi 533AV - Jm-0 Col 206AV N1258 Ta-0 Col 56AV N1548 Ka-0 Col 55AV N1266 Te-0 Ita 68AV N1550 Kar-1 Cvi 531AV - Tokushima Ita 258AV - Kar-2 Cvi 532AV - Tsu-0 Ita 91AV N1564

Kas-1 Ita

108AV N903 WestKar-3 Cvi

709AV Kin-0

Ita 209AV N1272 WestKar-4

Cvi 710AV

Kn-0 Ita 70AV N1286 Wil-1 Col 72AV N1594 Ko-2 Bay 46AV N1288 Ws Ita 244AV - Kondara Cvi 190AV N916 Ws-0 Bay 84AV N915 Kyr-1 Cvi 538AV - Yo-0 Ita 250AV N1622 Ldv-5 Col 11AV - Zal-1 Cvi 536AV - Ler-1 Ita 213AV N1642 Zal-3 Cvi 537AV - Lip-0 Ita 63AV N1336

a. Representive haplotype according to Figure S7 b. Accession number according to INRA Versailles Genomic Resource Centre

(http://dbsgap.versailles.inra.fr/vnat/) c. Accession number according to NASC, European Arabidopsis Stock Centre

(http://arabidopsis.info)

Pineau Supp. Table S2

Table S3. Characterization of Shahdara x frd3-7 F2 plants Perl staininga genotypeb N positive frd3/frd3 2 positive frd3/Sha 34 negative Sha/Sha 8 negative frd3/frd3 9 negative frd3/Sha 5

a. Fe accumulation is visualized by Perls’ stain [21] of intact roots of plants grown on compost for 3 weeks. Only frd3-7 plants accumulate Fe in the xylem

b. Genotype determined for the presence or absence of T-DNA using primers LBb1 and FRD3exon12R or FRD3exon7F and FRD3exon12R, respectively.

Pineau Supp. Table S3

Table S4. List of markers Name Positiona Typeb REc Forward (5’-3’) Reverse (5’-3’) NGA172 786 400 MSAT AGCTGCTTCCTTATAGCGTCC CATCCGAATGCCATTGTTC MSAT302422 2 422 445 MSAT CGGAATTAAGAACCGAATTGA ACAGGAAGGCTTCTGGGAAT MSAT302503 2 503 754 MSAT TTGTTGATTGCCCCATTTTT AAGGAATGAAATGATACATTTGGAA CAPS2524188 2 524 188 CAPS TaqI CAACTCCGGCAGTGATTTTG AACCAATGACGTCTTTCCAGA CAPS2533212 2 533 212 CAPS StuI TGCTTCCCTGCTCGTTAAGT TGGGAAGATATGCTGCTGAA CAPS2557387 2 557 387 CAPS Hpy188I TGAAGCTGAAGCTTGGGAAT TACCGCAAAAGCAAAAGAGG CAPS2560983 2 560 983 CAPS MnlI CAACTCCATGAACTCCAATGAA GATGAATTGGCGGAGGAAG CAPS2564930 2 564 930 CAPS HinfI ACGCTGTCGCCATTAAAGAG GAACCAAAGTGAACCAAAAACA IND302567 in12d 2 566 751 INDEL ATATGGCTCTCCGGGCTATT ATGATCGTCCACGCAAGAAC CAPS302569 d 2 568 974 CAPS BsrI AGCTTTGGCTTTAGCTGCTG CGGAATTTACCTGGAGGTTG IND302570 del27 d 2 569 837 INDEL CGAGTAAAAGTTCACGAAAACCA CCGTCCAAACTTAAGACACCA IND302570 del28 d 2 570 150 INDEL TTCTATACCAAATTAACCCGAAAA TTCTCATTTTTCTTTGATATTTTCCA SNP2579477 2 579 477 SNP TGTTCGACAAGTCTACCATGAAA TTCTGTTTGTTGGTCAACGATT CAPS2587730 2 587 730 CAPS RsaI GGCAACATTTGGGCATACAT GGCTCTAGAACTTGCCTGGA CAPS2619300 2 619 300 CAPS AluI ATGTGGTGCCATCTGTAGCC ACCCGTAGTTGCTTCCACAC CER464947 3 109 833 MSAT CTCACCTTGCTGGAGTCCG GTAAAGGTTTAGGCAGAGTGTG ATHCHIB2 3 963 350 MSAT GGATCCAAGTGCTCATATATAC CTTTCGTTTCTAAATATGAGAAGC CAPS4942156 4 942 156 CAPS HinfI TGGAAGCTCTAGAAACGATCG ACCACCTAAACCGAGAATTGG CAPS7012599 7 125 259 CAPS ClaI CACCTAACAGTTCACACCAA CCTTGATTTCCCTACAACAG MSAT3.19 8 808 167 MSAT TAATTCGATCCAATTGACAT TGGCTTGGCACAAAC

a. Position on chromosome 3 in bp. b. CAPS for Cleaved Amplified Polymorphic Sequence; INDEL for insertion or deletion; MSAT for microsatellite;

SNP for Single Nucleotide Polymorphism c. RE for Restriction Enzyme used for CAPS markers d. Markers used for the haplotyping of AtFRD3 in accessions of A. thaliana

Pineau Supp. Table S4

Table S5. List of primers

Application Primer name Forward (5’-3’) Reverse (5’-3’) Ref. seq. & cloning FRD3_promo1F TGACGTTGATGGCTTGAAAA a sequencing FRD3_promo2F TTTGGACGGAAATAAAGAGCA a sequencing FRD3_exon 2F GGCAGAGGAAGACACGATGG a sequencing FRD3_intron 4F GCCTTAATGCGTGAAACAGTC a sequencing FRD3_exon 12R TTCCTGTTGCCATCCTGCAT a seq. & cloning FRD3_post3'R CATTCCAAAAAGTGCAAGGAA a cDNA modification FRD3-V417I TCCTTGGAGAAAATTCCGGCACCAA TTGGTGCCGGAATTTTCTCCAAGGA a cDNA modification FRD3-LP-NS TTTCTGCATGAACAGGACTGGCTTTGTTTGCT AGCAAACAAAGCCAGTCCTGTTCATGCAGAAA a cDNA modification FRD3-L117P ACAAAGCCAATCCTGTTCATGCAGA TCTGCATGAACAGGATTGGCTTTGT a cDNA modification FRD3-N116S GCATGAACAAGACTGGCTTTGTTTG CAAACAAAGCCAGTCTTGTTCATGC a Q-RTPCR AtIRT1 (At4g19690) CGGTTGGACTTCTAAATGC CGATAATCGACATTCCACCG 35 Q-RTPCR AtFRO2 (At1g01580) GCGACTTGTAGTGCGGCTATG CGTTGCACGAGCGATTCTTG 35 Q-RTPCR AtFRD3 (At3g08040) CGAGGACCATAGCAGTGACG CAGCAAACGAACAAGCCAGA a Q-RTPCR Clathrin (At4g24550) AGCATACACTGCGTGCAAAG TCGCCTGTGTCACATATCTC 35 Q-RTPCR ACT2/ACT8

(At3g18780/At1g49240) GGTAACATTGTGCTCAGTGGTGG AACGACCTTAATCTTCATGCTGC 34

Q-RTPCR PP2A (At1g13320) TAACGTGGCCAAAATGATGC GTTCTCCACAACCGCTTGGT 36 Q-RTPCR At5g12240 AGCGGCTGCTGAGAAGAAGGT TCTCGAAAGCCTTGCAAAATCT 36 ASEb PyroSNP1-2 TGCTGTGGCTGGTTGGTAT GGAAGACACGATGGAGAAGATG a ASEb PyroSNP1-2_Seq CAAGTATAGTTTCTGCATGA a ASEb PyroSNP3 TGCTGTGGCTGGTTGGTAT GGAAGACACGATGGAGAAGATG a ASEb PyroSNP3_Seq GCATTTCTTCACCTACAAGT a ASEb PyroSNP4 CCCAAAGCTCGTATGCTCAAGT GCCAATCTTGTTCATGCAGAAACT a ASEb PyroSNP4_Seq CGCCTAGAAGCAACTT a T-DNA testing LBb1 GCGTGGACCGCTTGCTGCAACT T-DNA testing FRD3_exon 12R TTCCTGTTGCCATCCTGCAT T-DNA testing FRD3_exon 7F CGAGGACCATAGCAGTGACG

a. present study b. ASE for Allele-Specific Expression

Pineau Supp. Table S5

0

5

10

15

20

25

30

1-1.5

1.5-2

2-2.5

2.5-3

3-3.5

3.5-4

4-4.5

4.5-5

5-5.5

5.5-6

6-6.5

6.5-7

7-7.5

7.5-8

8-8.5

8.5-9

Primary root length (cm)

frequ

ency

(%)

1 µM Zn150 µM Zn

Bay-0

Sha

Figure S1

Sha

Bay-0

A B

Figure S2

1 2 3 4 5HIF044 HIF338

1 2 3 4 51 2 3 4 5HIF004

C

**

0

10

20

30

40

50

60

70

Rel

ativ

e P

rimar

y R

oot l

engt

h (%

)

**

D

HIF044

Sha0

10

20

30

40

50

60

70

Rel

ativ

e P

rimar

y R

oot l

engt

h (%

)

**

ZnT1 ZnT2

Bay

HIF004

Sha Bay

HIF338

Sha Bay

0

10

20

30

40

50

60

70

HIF004Bayx

HIF004Sha

Rel

ativ

e P

rimar

y R

oot l

engt

h (%

)

HIF004Bay HIF004Sha

a ab

Figure S3

1 2 3 4 5 1 2 3 4 5

NIL[Sha] NIL[Bay]

MSAT3.19

ATHCHIB2

NGA17233

CAPS7012599

CAPS6240557

MSAT302422CER464947

Figure S4

0

1

2

3

4

5

Col-0 frd3.7

NIL[Sha]BayP

rimar

y ro

ot le

ngth

(cm

)

Figure S5

Figure S6

FRD3_COL TGACGTTGATGGCTTGAAAAAGATGGCCTTATCTTGCGGAACAAACAATT FRD3_BAY TGACGTTGATGGCTTGAAAAAGATGGCCTTATCTTGCGGAACAAACAATT FRD3_SHA TGACGTTGATGGCTTGAAAAAGATGGCCTTATCTTGCGGAACAAACAATT FRD3_COL ACATACACGACACGCACTATATACAACTCACCTGTGTTGGTCTCTGTTGC FRD3_BAY ACATACACGACACGCACTATATACAACTCACCTGTGTTGGTCTCTGTTGC FRD3_SHA ACATACACGACACGCACGATATACAACTCACCTGTGTTGGTCTCTGTTGC FRD3_COL CATCTTTTATGTTATTGTTTTCCGACTGTCGCCTTCCTTTAACTAATATT FRD3_BAY CATCTTTTATGTTATTGTTTTCCGACTGTCGCCTTCCTTTAACTAATATT FRD3_SHA CATCTTTTATGTTATTGTTTTCCGACTGTCGCCTTCCTTTAACTAATATT FRD3_COL ATAATTTTAAAGATGTTCATAAATCACAGTAGAAAGCTTGTTTTTGCTAA FRD3_BAY ATAATTTTAAAGATGTTCATAAATCACAGTAGAAAGCTTGTTTTTGCTAA FRD3_SHA ATAATTTTAAAGATGTTCATAAATCACAGTAGAAAGCTTGTTTTTGCTAA FRD3_COL AATGAACATGACACGGATCATACAAAAAATATATTTTACACTATAGCTAT FRD3_BAY AATGAACATGACACGGATCATACAAAAAATATATTTTACACTATAGCTAT FRD3_SHA AATGAACATGACACGGATCATACAAAAAATATATTTTACACTATAGCTAT FRD3_COL ATACCGATTTAATCTTAGGTACTTTGAATCGTGCTAAAACTAAACGGCCT FRD3_BAY ACACCGATTTAATCTTAGGTACTTTGAATCGTGCTAAAACTAAACGGCCT FRD3_SHA ATACCGATTTAATCTTAGGTACTTTGAATCGTGCTAAAACTAAACGGCCT FRD3_COL TCTCAAAACCCTCCCTCTTTTCCTCCCTCCCTCAGAAACCCCTCCATCGA FRD3_BAY TCTCAAAACCCTCCCTCTTTTCCTCCCTCCCTCAGAAACCCCTCCATCGA FRD3_SHA TCTCAAAACCCTCCCTCTTTTCCTCCCTCCCGCAGAAACCCCTCCCTCGA FRD3_COL CAAATAACGTTATGCAATTCTCTAAACAATGCTCGACAAGCATGTGTTTT FRD3_BAY CAAATAACGTTATGCAATTCTCTAAACAATGCTCGACAAGCATGTGTTTT FRD3_SHA CAAATAACGTTATGCAATTCTCTAAACAATGCTCGACAAGCATGTGTTTT FRD3_COL TAGTAATGCTACAACTTATTTCTCTTTTCAACGTCCTAAGAGGCATCAAA FRD3_BAY TAGTAATGCTACAACTTATTTCTCTTTTCAACGTCCTAAGAGGCATCAAA FRD3_SHA TAGTAATGCTACAACTTATTTCTCTTTTCAACGTCCTAAGAGGCATCAAA FRD3_COL AAGATCAAAGATCTTGGAACCGAGGTCCTATGCAAGGAATCATTATAAAG FRD3_BAY AAGATCAAAGATCTTGGAACCGAGGTCCTATGCAAGGAATCATTATAAAG FRD3_SHA AAGATCAAAGATCTTGGAACCGAGGTCCTATGCAAGGAATCATTATAAAG FRD3_COL TACCATGTTATTTTTTTAAATAACATCGTTTTCTTAATATAATTTATAAA FRD3_BAY TACCATGTTATTTTTTTAAATAACATCGTTTTCTTAATATAATTTATAAA FRD3_SHA TACCATGTTATTTTTTTAAATAACATTGTTTTCTTAATATAATTTACAAA FRD3_COL TACCGTTATTTTTACCGAAATTTCATATATATGTCAGTTTTATACTTTGT FRD3_BAY TACCGTTATTTTTACCGAAATTTCATATATATGTCAGTTTTATACTTTGT FRD3_SHA TACCGTTATTTTTACCGAAATTTCATATCTATGTCAGTTTTATACTTTGT FRD3_COL ACGATAACGCCAAAAACTTTAATTATCGCCAAAATTGTAAACGGTATTTT FRD3_BAY ACGATAACGCCAAAAACTTTAATTATCGCCAAAATTGTAAACGGTATTTT FRD3_SHA ACGATAACGCCAAAAACTTTAATTATCGCCAAAATTGTAAACGGTATTTT FRD3_COL CGTCGTTTAGTTATTTACCAAAAATAAAATGACGATTGCAACTTATTTAG FRD3_BAY CGTCGTTTAGTTATTTACCAAAAATAAAATGACGATTGCAACTTATTTAG FRD3_SHA CGTCGTTTAGTTATTTACCAAAAATAAAATGACGATTGCAACTTCTTTAG FRD3_COL TTAAAATACAAAAAAAAACTAATATATTAATTGAGCGGACGGAATTTTTT FRD3_BAY TTAAAATACAAAAAAAA-CTAATATATTAATTGAGCGGACGGAATTTTTT FRD3_SHA TTAAAATACAAAAAAA--CTAATATATTAATTGAGCGGACGAAATTTTTT FRD3_COL TCCAAAATCCCGATGTGTAAATATGAGAACGTTTCGAGGATAACTTACAA FRD3_BAY TCCAAAATCCCGATGTGTAAATATGAGAACGTTTCGAGGATAACTTACAA FRD3_SHA TCCAAAATCCCGATGTGTAAATATGAGAACGTTTCGAGGATAACTTACAA FRD3_COL ATTAAACATTAATAAAAATGATAAAGTGTAGTTAGGAGCTAAATTGTGAT FRD3_BAY ATTAAACATTAATAAAAATGATAAAGTGTAGTTAGGAGCTAAATTGTGAT FRD3_SHA ATTAAACATTAATCAAAATGATAAAGTGTAGTTAGGAGCTAAATTGTGAT FRD3_COL AGTAAACATCTATCTCTAATATTATTAAATGAATTATAATACTATTTTAA FRD3_BAY AGTAAACATCTATCTCTAATATTATTAAATGAATTATAATACTATTTTAA FRD3_SHA AGTAAACATCTATCTCTAATATTATTAAATGAATTATAATAATATTTTAA FRD3_COL TCATAGTATTAAATTTCTTTAATTAAAAATATAAATAATTTCAATTTAAT FRD3_BAY TCATAGTATTAAATTTCTTTAATTAAAAATATAAATAATTTCAATTTAAT FRD3_SHA TTATAGTATCAAATTTCTTTAATTAAAAATATAAATAATTTCAATTTAAT FRD3_COL TCTATACCAAATTAACCCGAAAATATTTTATCTAACATACACAAAGACAC FRD3_BAY TCTATACCAAATTAACCCGAAAATATTTTATCTAACATACACAAGGACAC FRD3_SHA TCTATACCAAATTAACCCAAAAATATTCTATCTAACATA-ACAAAGACAC FRD3_COL ATAAAAGTTTTGATAACTGCCTAAAAAAATAAGCTTTTGAATTATTAATT FRD3_BAY ATAAAAGTTTTGATAACTGCCTAAAAAAATAAGCTTTTGAATTATTAATT FRD3_SHA ATAAAAGTTTTGATAACTGCCTAAAAAAATAAGCTTTTAAATTATTAATT FRD3_COL AGTTGTTATTCAATGATAAAATAACATTATTTGTCAACTAGTGAATTCCA FRD3_BAY AGTTGTTATTCAATGATAAAATAACATTATTTGTCAACTAGTGAATTCCA FRD3_SHA AGATAT----------------------------CAACTAGTGAATTTCA FRD3_COL ATTACGCAAAATGATTCACTTTTTTAGTGGAAAATATCAAAGAAAAATGA FRD3_BAY ATTACGCAAAATGATTCACTTTTTTAGTGGAAAATATCAAAGAAAAATGA FRD3_SHA ATTACGCAAAATGATTCACTTTTTTAGTGGAAAATATCAAAGAAAAATGA FRD3_COL GAAGTTTATATGAAAATAAACTCTTTCCCACTATGATGAATACATGTAAG FRD3_BAY GAAGTTTATATGAAAATAAACTCTTTCCCACTATGATGAATACATGTAAG FRD3_SHA GAAGTTTATATGAAAATAAACTCTTTCCCACTATGATGAATACATGTAAG FRD3_COL AAAACTTTCATGAAAAGAAAACTTATTTACTCAATATAAAAATAGAAGAC FRD3_BAY AAAACTTTCATGAAAAGAAAACTTATTTACTCAATATAAAAATAGAAGAC FRD3_SHA AAAACTTTCATGAAAAGAAAACTTATTTACTCAATATAAAAATAGAAGAC FRD3_COL TCTTTATCTTTCACGAGTAAAAGTTCACGAAAACCATATTTTCCTATTGA FRD3_BAY TCTTTCTCTTTCACGAGTAAAAGTTCACGAAAACCATATAATCCTATTGA FRD3_SHA TCTTTATCTTTCACGAGTAAAAGTTCACGAAAACCATATTTTCCTATTGA FRD3_COL TTAAAGAAATCATAGAAGTTAAAATAATCAACAAGGGCAAGCCAAAAACT FRD3_BAY TTAA---------------------------CAAGGGCAAGCCGAAAACT FRD3_SHA TTAAAGAAATCATAGAAGTTAAAATAATCAACAAGGGCAAGCCAAAAACT

FRD3_COL TCTAGTGTGGGATTTACTTAATAGAAGTATATATATTACGATGTTTATGC FRD3_BAY TCTAGTG--GGATTTACTTAATAGAAGTATATATATTACGATGTTTATGC FRD3_SHA TCTAGTGTGGGATTTACTTAATAGAAGTATATATATTACGATGTTTATGC FRD3_COL GTACCTATTTTCCCTCAATGAGAAGAGAAATTCCATAATATTGGTGTCTT FRD3_BAY GTACCTATTTTCCCTCAATGAGAAGAGAAATTCCATAATATTGGTGTCTT FRD3_SHA GTACCTATTTTCCCTCAATGAGAAGAGAAATTCCATAATATTGGTGTCTT FRD3_COL AAGTTTGGACGGAAATAAAGAGCAGCAAAAAAGTTAGGGAAGGAAACCTT FRD3_BAY AAGTTTGGACGGAAATAAAGAGCAGCAAAAAAGTTAGGGAAGGAAACCTT FRD3_SHA AAGTTTGGACGGAAATAAAGAGCAGCAAAAAAGTTAGGGAAGGAAACCTT FRD3_COL TGTTTTCTTCAATAATTATAGAAAATAATTTCTTTTATTG--ATTTAGAT FRD3_BAY TGTTTTCTTCAATAATTATAGAAAATAATTTAATTTATTTTTATTTAGAT FRD3_SHA TGTTTTCTTCAATAATTATAGAAAATAATTTCTTTTATTG--ATTTAGAT FRD3_COL ATTAAATAAGCAAAGATATGCATGCTCATTACGTGTCTATAAATAAAAAC FRD3_BAY ATTAAATAAGCAAAGATATGCATGCTCATTACGTGTCTATAAATAAAAAC FRD3_SHA ATTAAATAAGCAAAGATATGCATGCTCATTACGTGTCTATAAATAAAAAC FRD3_COL ACGTTTGTACATAGCATCTACTATAAACGTTCCTTTTGCTTCCCCGATTC FRD3_BAY ACGTTTGTACATAGCATCTACTATAAACGTTCCTTTTGCTTCCCCGATTC FRD3_SHA ACGTTTGTACATAGCATCTACTATAAACGTTCCTTTTGCTTCCCCGATTC FRD3_COL TTCGAAACACTTATTGATATCTTCAGACACAACAAATTAATTACAGAGAC FRD3_BAY TTCGAAACACTTATTGATATCTTCAGACACAACAAATTAATTACAGAGAC FRD3_SHA TTCGAAACACTTATTGATATCTTCAGACACAACAAATTAATTACAGAGAC FRD3_COL AGTTACAGAGGAAAAAGATCTATGACGGAAACTGGTGATGATCTTGCTAC FRD3_BAY AGTTACAGAGGAAAAAGATCTATGACGGAAACTGGTGATGATCTTGCTAC FRD3_SHA AGTTACAGAGGAAAAAGATCTATGACGGAAACTGGTGATGATCTTGCTAC FRD3_COL GGTGAAGAAGCCAATCCCATTTCTCGTTATCTTCAAAGATTTAAGGTGTG FRD3_BAY GGTGAAGAAGCCAATCCCATTTCTCGTTATCTTCAAAGATTTAAGGTGTG FRD3_SHA GGTGAAGAAGCCAATCCCATTTCTCGTTATCTTCAAAGATTTAAGGTGTG FRD3_COL TGTTTATGTATTCATGAAATGGTGATGAAATTTTTGAAAGAAGTGATGCA FRD3_BAY TGTTTATGTATTCATGAAATGGTGATGAAATTTTTGAAAGAAGTGATGCA FRD3_SHA TGTTTATGTATTCATGAAATGGTGATGAAATTTTTGAAAGAAGTGATGCA FRD3_COL TAACATTAGTTTATTTATGTAAAATTGCAGACATGTATTCAGTAGGGACA FRD3_BAY TAACATTAGTTTATTTATGTAAAATTGCAGACATGTATTCAGTAGGGACA FRD3_SHA TAACATTAGTTTATTTATGTAAAATTGCAGACATGTATTCAGTAGGGACA FRD3_COL CAACTGGGCGAGAGATTCTAGGCATCGCGTTTCCAGCAGCTTTGGCTTTA FRD3_BAY CAACTGGGCGAGAGATTCTAGGCATCGCGTTTCCAGCAGCTTTGGCTTTA FRD3_SHA CAACTGGGCGAGAGATTCTAGGCATCGCGTTTCCAGCAGCTTTGGCTTTA FRD3_COL GCTGCTGATCCAATCGCTTCTCTGATTGATACCGCTTTTGTCGGGCGTTT FRD3_BAY GCTGCTGATCCAATCGCTTCTCTGATTGATACCGCTTTTGTCGGGCGTTT FRD3_SHA GCTGCTGATCCAATCGCTTCTCTGATTGATACCGCTTTTGTCGGGCGTTT FRD3_COL AGGAGCGGTTCAGCTAGCGGCGGTTGGAGTTTCCATTGCCATATTCAATC FRD3_BAY AGGAGCGGTTCAGCTAGCGGCGGTTGGAGTTTCCATTGCCATATTCAATC FRD3_SHA AGGAGCGGTTCAGCTAGCGGCGGTTGGAGTTTCCATTGCCATATTCAATC FRD3_COL AAGCTTCTAGAATTACGATATTCCCACTTGTGAGCCTCACAACTTCATTT FRD3_BAY AAGCTTCTAGAATTACGATATTCCCACTTGTGAGCCTCACAACTTCATTT FRD3_SHA AAGCTTCTAGAATTACGATATTCCCACTTGTGAGCCTCACAACTTCATTT FRD3_COL GTGGCAGAGGAAGACACGATGGAGAAGATGAAAGAAGAAGCAAACAAAGC FRD3_BAY GTGGCAGAGGAAGACACGATGGAGAAGATGAAAGAAGAAGCAAACAAAGC FRD3_SHA GTGGCAGAGGAAGACACGATGGAGAAGATGAAAGAAGAAGCAAACAAAGC FRD3_COL CAATCTTGTTCATGCAGAAACTATACTTGTTCAAGATTCTTTGGAAAAGG FRD3_BAY CAATCTTGTTCATGCAGAAACTATACTTGTTCAAGATTCTTTGGAAAAGG FRD3_SHA CAGTCCTGTTCATGCAGAAACTATACTTGTTCAAGATTCTTTGGAAAAAG FRD3_COL GCATTTCTTCACCTACAAGTAACGATACCAACCAGCCACAGCAACCTCCA FRD3_BAY GCATTTCTTCACCTACAAGTAATGATACCAACCAGCCACAGCAACCTCCA FRD3_SHA GCATTTCTTCACCTACAAGTAATGATACCAACCAGCCACAGCAACCTCCA FRD3_COL GGTAAATTCCGCATATCTCACTCGACATTGATAACTTTTATTAAAGTTTC FRD3_BAY GGTAAATTCCGCATATCTCACTCGACATTGATAACTTTTATTAAAGTTTC FRD3_SHA GGTAAATTCCGCATATCTCACTCGACATTGATAACTTTTATTAAAGTTTC FRD3_COL GATTGTTTTTTTACTGTTGGTTTCTTCTCTCGATCTCTTTTGTTTCAATT FRD3_BAY GATTGTTTTTTTACTGTTGGTTTCTTCTCTCGATCTCTTTTGTTTCAATT FRD3_SHA GATTGTTTTTTTACTGTTGGTTTCTTCTCTCGATCTCTTTTGTTTCAATT FRD3_COL TGTTGTTTTTTTGGTTGTATTAAACTTAGCTCCGGATACAAAGTCAAATA FRD3_BAY TGTTGTTTTTTTGGTTGTATTAAACTTAGCTCCGGATACAAAGTCAAATA FRD3_SHA TGTTGTTTTTTTGGTTGTATTAAACTTAGCTCCGGATACAAAGTCAAATA FRD3_COL GCGGAAACAAATCGAATAAAAAGGAGAAGAGGACCATTAGAACAGCATCA FRD3_BAY GCGGAAACAAATCGAATAAAAAGGAGAAGAGGACCATTAGAACAGCATCA FRD3_SHA GCGGAAACAAATCGAATAAAAAGGAGAAGAGGACCATTAGAACAGCATCA FRD3_COL ACAGCTATGATCTTGGGGTTAATCCTTGGCCTTGTGCAAGCTATTTTCTT FRD3_BAY ACAGCTATGATCTTGGGGTTAATCCTTGGCCTTGTGCAAGCTATTTTCTT FRD3_SHA ACAGCTATGATCTTGGGGTTAATCCTTGGCCTTGTGCAAGCTATTTTCTT FRD3_COL GATTTTCAGTTCAAAGTTGCTTCTAGGCGTCATGGGAGTGAAACCAGTAA FRD3_BAY GATTTTCAGTTCAAAGTTGCTTCTAGGCGTCATGGGAGTGAAACCAGTAA FRD3_SHA GATTTTCAGTTCAAAGTTGCTTCTAGGCGTCATGGGAGTGAAACCAGTAA FRD3_COL GTTTTCAGAAATATACATATTTTGTTGGGATCTATAGCATAAAATGTTTT FRD3_BAY GTTTTCAGAAATATACATATTTTGTTGGGATCTATAGCATAAAATGTTTT FRD3_SHA GTTTTCAGAAATATACATATTTTGTTGGGATCTATAGCATAAAATGTTTT FRD3_COL GACTAATTTGAGTTGAATTTGGATAACAGAATTCACCAATGTTATCACCA FRD3_BAY GACTAATTTGAGTTGAATTTGGATAACAGAATTCACCAATGTTATCACCA FRD3_SHA GACTAATTTGAGGTGAATTTGGATAACAGAATTCACCAATGTTATCACCA FRD3_COL GCACACAAGTACTTGAGCATACGAGCTTTGGGGGCTCCTGCATTGCTTCT FRD3_BAY GCACACAAGTACTTGAGCATACGAGCTTTGGGGGCTCCTGCATTGCTTCT FRD3_SHA GCACACAAGTACTTGAGCATACGAGCTTTGGGGGCTCCTGCATTGCTTCT

Figure S6. Continued

FRD3_COL ATCTCTTGCTATGCAAGGCATCTTTCGTGGATTCAAGGACACCAAAACTC FRD3_BAY ATCTCTTGCTATGCAAGGCATCTTTCGTGGATTCAAGGACACCAAAACTC FRD3_SHA ATCTCTTGCTATGCAAGGCATCTTTCGTGGATTCAAGGACACCAAAACTC FRD3_COL CTCTCTTTGCCACTGGTAATTAAGTTGTTAACTTAGATCATCTTTAATGA FRD3_BAY CTCTCTTTGCCACTGGTAATTAAGTTGTTAACTTAGATCATCTTTAATGA FRD3_SHA CTCTCTTTGCCACTGGTAATTAAGTTGTTAAATTAGATCATCTTTAATGA FRD3_COL TCACTCTCCTTACTTCTTATAATAT-----------------TTTGCCTT FRD3_BAY TCACTCTCCTTACTTCTTATAATATGTTAGCTAATATAATATTTTGCCTT FRD3_SHA TCACTTTTCTTACTTCTTATAATATGTTAGCTAATATAATATCTTGCCTT FRD3_COL AATGCGTGAAACAGTCGTAGCAGATGTTATCAACATAGTTCTCGACCCCA FRD3_BAY AATGCGTGAAACAGTCGTAGCAGATGTTATCAACATAGTTCTCGACCCCA FRD3_SHA AATGCGTGAAACAGTCGTAGCAGATGTTATCAACATAGTTCTCGACCCCA FRD3_COL TCTTCATTTTTGTGCTTCGTCTAGGGATCATCGGTGCAGCCATTGCCCAT FRD3_BAY TCTTCATTTTTGTGCTTCGTCTAGGGATCATCGGTGCAGCCATTGCCCAT FRD3_SHA TCTTCATTTTTGTGCTTCGTCTAGGGATCATCGGTGCAGCCATTGCCCAT FRD3_COL GTCATTTCTCAGTAAGAGAAATCACTAAAAAAATTCCACACATGCAAAAG FRD3_BAY GTCATTTCTCAGTAAGAGAAATCACTAAAAAAATTCCACACATGCAAAAG FRD3_SHA GTCATTTCTCAGTAAGAGAAATCACTAAAAAAATTCCACACATGCAAAAG FRD3_COL TGATCATTATTGAACAAAATCGCTAGGCGCACTCTTGTTTTTCTACAGCT FRD3_BAY TGATCATTATTGAACAAAATCGCTAGGCGCACTCTTGTTTTTCTACAGCT FRD3_SHA TGATCATTATTGCACAAAATCGCTAGGCGCACTCTTGTTTTTCTACAGCT FRD3_COL ATAAATAGACTTGTGAAGTCATAACCTCAAACAAAAACAAATGATTTGTT FRD3_BAY ATAAATAGACTTGTGAAGTCATAACCTCAAACAAAAACAAATGATTTGTT FRD3_SHA ATAAATAGACTTGTGAAGTCATAACCTCAAACAAAAACTAATGATTTGTT FRD3_COL TGTGTACGTGAAGGTACTTCATGACTCTAATATTGTTCGTCTTCCTCGCA FRD3_BAY TGTGTACGTGAAGGTACTTCATGACTCTAATATTGTTCGTCTTCCTCGCA FRD3_SHA TGTGTACGTGAAGGTACTTCATGACTCTAATATTGTTCGTCTTCCTCGCA FRD3_COL AAGAAAGTTAATTTGATTCCACCAAACTTCGGGGATTTGCAGTTTGGAAG FRD3_BAY AAGAAAGTTAATTTGATTCCACCAAACTTCGGGGATTTGCAGTTTGGAAG FRD3_SHA AAGAAAGTTAATTTGATTCCACCAAACTTCGGGGATTTGCAGTTTGGAAG FRD3_COL GTTCCTTAAAAATGGTACGTATGGATGCATATTTATTAAAAGTTGTGGTT FRD3_BAY GTTCCTTAAAAATGGTACGTATGGATGCATATTTATTAAAAGTTGTGGTT FRD3_SHA GTTCCTTAAAAATGGTACGTATGGATGCATATTTATTAAAAGTTGTGGTT FRD3_COL CTTGCAATAATATTTTTTTTT-AAAAACAAGATCCGTCGTAGGAGCTAAT FRD3_BAY CTTGCAATAATATTTTTTTTTTAAAAACAAGATCCGTCGTAGGAGCTAAT FRD3_SHA CTTGCAATAATAATTTTTTTT-AAAAACAAGATCCGTCGTAGGAGCTAAT FRD3_COL GCACAGAGTCCAAAAATAAATTAACAAAAAATTTATCTATATAATAATAG FRD3_BAY GCACAGAGTCCAAAAATAAATTAACAAAAAATTTATCTATATAATAATAG FRD3_SHA GCACAGAGTCCAAAAATAAATTAACAAAAAATTTATCTATATAATAATAG FRD3_COL AATTCAATCAAATAAGGTCTATATTTAAAATATTGAATATTTTGAAATAT FRD3_BAY AATTCAATCAAATAAGGTCTATATTTAAAATATTGAATATTTTGAAATAT FRD3_SHA AATTCAATCAAATAAGGTCTATATTTAAAATATTGAATATTTTGAAATAT FRD3_COL ATAGTTAAGAAAATGAGAAATGTGGATATATGTCTAACAAGTATAGTATT FRD3_BAY ATAGTTAAGAAAATGAGAAATGTGGATATATGTCTAACAAGTATAGTATT FRD3_SHA ATAGTTAAGAAAATGAGAAATGTGGATATATGTCTAACAAGTATAGTATT FRD3_COL AAAAATGAAAGGGCTACTATTGCTGGCGAGGACCATAGCAGTGACGTTTT FRD3_BAY AAAAATGAAAGGGCTACTATTGCTGGCGAGGACCATAGCAGTGACGTTTT FRD3_SHA AAAAATGAAAGGGCTACTATTGCTGGCGAGGACCATAGCAGTGACGTTTT FRD3_COL GTCAGACCTTAGCAGCAGCAATGGCGGCGCGGCTGGGTACAACACCAATG FRD3_BAY GTCAGACCTTAGCAGCAGCAATGGCGGCGCGGCTGGGTACAACACCAATG FRD3_SHA GTCAGACCTTAGCAGCAGCAATGGCGGCGCGGCTGGGTACAACACCAATG FRD3_COL GCTGCTTTTCAGATTTGTTTACAAGTATGGTTAACTTCTTCTCTTCTCAA FRD3_BAY GCTGCTTTTCAGATTTGTTTACAAGTATGGTTAACTTCTTCTCTTCTCAA FRD3_SHA GCTGCTTTTCAGATTTGTTTACAAGTATGGTTAACTTCTTCTCTTCTCAA FRD3_COL TGATGGTCTTGCCGTTGCTGGTCAGGTAATCATGTTTTCTCGTTGTATTA FRD3_BAY TGATGGTCTTGCCGTTGCTGGTCAGGTAATCATGTTTTCTCGTTGTATTA FRD3_SHA TGATGGTCTTGCCGTTGCTGGTCAGGTAATCATGTTTTCTCGTTGTATTA FRD3_COL ATTTATGTATAGTTTATATGGTTGATCAAGTTGTATGTAGAAAATGATCA FRD3_BAY ATATATGTATAGTTTATATGGTTGATCAAGTTGTATGTAGAAAATGATCA FRD3_SHA ATATATGTATAGTTTATATGGTTGATCAAGTTGTATGTAGAAAATGATCA FRD3_COL TTCAATACGTTGCAGGCGATTCTGGCTTGTTCGTTTGCTGAGAAGGACTA FRD3_BAY TTCAATACGTTGCAGGCGATTCTGGCTTGTTCGTTTGCTGAGAAGGACTA FRD3_SHA TTCAATACGTTGCAGGCGATTCTGGCTTGTTCGTTTGCTGAGAAGGACTA FRD3_COL TAACAAAGTGACTGCTGTTGCATCCCGTGTTCTACAGGTTCGGTCCAAAA FRD3_BAY TAACAAAGTGACTGCTGTTGCATCCCGTGTTCTACAGGTTCGGTCCAAAA FRD3_SHA TAACAAAGTGACTGCTGTTGCATCCCGTGTTCTACAGGTTCGGTCCAAAA FRD3_COL ATCACATTACCAAACCTTTCTTTAAAAATAAAATAATTGTGTAACTAAAA FRD3_BAY ATCACATTACCAAACCTTTCTTTAAAAATAAAATAATTGTGAAACTAAAA FRD3_SHA ATCACATTACCAAACCTTTCTTTAAAAATAAAATAATTGTGTAACTAAAA FRD3_COL CAGAAATGAATTTGATACGCAGATGGGTTTTGTGTTAGGACTTGGACTGT FRD3_BAY CAGAAATGAATTTGATACGCAGATGGGTTTTGTGTTAGGACTTGGACTGT FRD3_SHA CAGAAATGAATTTGATACGCAGATGGGTTTTGTGTTAGGACTTGGACTGT FRD3_COL CCGTTTTTGTTGGACTAGGTCTCTACTTTGGTGCCGGAGTTTTCTCCAAG FRD3_BAY CCGTTTTTGTTGGACTAGGTCTCTACTTTGGTGCCGGAATTTTCTCCAAG

FRD3_COL GACCCTGCTGTTATTCACCTCATGGCCATCGGAATACCGGTAACTAATAA FRD3_BAY GACCCTGCTGTTATTCACCTCATGGCCATCGGAATACCGGTAACTAATAA FRD3_SHA GACCCTGCTGTTATTCACCTCATGGCCATCGGAATACCGGTAACTAATAA FRD3_COL TCAAATAATAATTACTATAGTATAAAAATCATTTTAAAAGAATTTTACTA FRD3_BAY TCAAATAATAATTACTATAGTATAAAAATCATTTTAAAAGAATTTTACTA FRD3_SHA TCAAATAATAATTACTATAGTATAAAAATCATTTTAAAAGAATTTTACTA FRD3_COL ATGAGAAGAGGTTATATATATTTATGCAGTTTATAGCAGCAACGCAGCCA FRD3_BAY ATGAGAAGAGGTTATATATATTTATGCAGTTTATAGCAGCAACGCAGCCA FRD3_SHA ATGAGAAGAGGTTATATATATTTATGCAGTTTATAGCAGCAACGCAGCCA FRD3_COL ATAAACTCTCTCGCCTTTGTATTGGATGGAGTCAATTTTGGAGCATCTGA FRD3_BAY ATAAACTCTCTCGCCTTTGTATTGGATGGAGTCAATTTTGGAGCATCTGA FRD3_SHA ATAAACTCTCTCGCCTTTGTATTGGATGGAGTCAATTTTGGAGCATCTGA FRD3_COL TTTTGCTTACACTGCATACTCCATGGTATGCACACTATATATACTATGAA FRD3_BAY TTTTGCTTACACTGCATACTCCATGGTATGCACACTATATATACTATGAA FRD3_SHA TTTTGCTTACACTGCATACTCCATGGTATGCACACTATATATACTATGAA FRD3_COL ATGATTAAAATTCCTTTTTTTTTTTTTGAAATGACTTAAACTTTGTCTAT FRD3_BAY ATGATTAAAATTCCTTCTTTTTTTTTTGAAATGACTTAAACTTTGTCTAT FRD3_SHA ATGATTAAAATTCCTTTTTTTTTTTT-GAAATGACTTAAACTTTGTCTAT FRD3_COL CTTTTTTTCTTGTAATCCAATTATGATAAATCAGGTGGGAGTGGCGGCCA FRD3_BAY CTTTTTTTCTTGTAATCCAATTATGATAAATCAGGTGGGAGTGGCGGCCA FRD3_SHA CTTTTTTTCTTGTAATCCAATTATGATAAATCAGGTGGGAGTGGCGGCCA FRD3_COL TAAGCATTGCAGCAGTAATATATATGGCAAAGACCAATGGTTTCATAGGA FRD3_BAY TAAGCATTGCAGCAGTAATATATATGGCAAAGACCAATGGTTTCATAGGA FRD3_SHA TAAGCATTGCAGCAGTAATATATATGGCAAAGACCAATGGTTTCATAGGA FRD3_COL ATATGGATAGCTCTTACAATCTATATGGCTCTCCGGGCTATTACTGGAAT FRD3_BAY ATATGGATAGCTCTTACAATCTATATGGCTCTCCGGGCTATTACTGGAAT FRD3_SHA ATATGGATAGCTCTTACAATCTATATGGCTCTCCGGGCTATTACTGGAAT FRD3_COL TGCCAGGTATTTAAATTGGGCCTT------------TACTATAGCCCACT FRD3_BAY TGCCAGGTATTTTTATTGGGTCTTAATGGGCCTTTACATAATAGCCCACT FRD3_SHA TGCCAGGTATTTAAATTGGGCCTT------------TACTATAGCCCACT FRD3_COL ATAGTAGAAGCAGTATTTGACTGAGTGTTTGAATTTATGCAGGATGGCGA FRD3_BAY ATAGTAGAAGCAGTATTTGACTGAGTGTTTGAATTTATGCAGGATGGCAA FRD3_SHA ATAGTAGAAGCAGTATTTGACTGAGTGTTTGAATTTATGCAGGATGGCGA FRD3_COL CAGGAACTGGACCGTGGAGGTTCTTGCGTGGACGATCATCCTCTTCATCT FRD3_BAY CAGGAACTGGACCTTGGAGGTTCTTGCGTGGACGATCATCCTCTTCATCT FRD3_SHA CAGGAACTGGACCGTGGAGGTTCTTGCGTGGACGATCATCCTCTTCATCT FRD3_COL TCCTAGGACTTAGTTTATTTATAACGAGTTGCATCTCTTCTTCCTTCTTC FRD3_BAY TCCTAGGACTTAGTTTATTTATAACGAGTTGCATCTCTTCTTCCTTCTTC FRD3_SHA TCCTAGGACTTAGTTTATTTATAACGAGTTGCATCTCTTCTTCCTTCTTC FRD3_COL GTTTTTGTTTATGGTTCTTGTGTTTGTTTTTCAACATTTTGTTCGAGAGA FRD3_BAY GTTTTTGTTTATGGTTCTTGTGTTTGTTTTTCAACATTTTGTTCGAGAGA FRD3_SHA GTTTTTGTTTATGGTTCTTGTGTTTGTTTTTCAACATTTTGTTCGAGAGA FRD3_COL CCGTTATCATATTATCAGTTTCACATAAATAATGCATATTTTTAAGTCAT FRD3_BAY CCGTTATCAT--TATCAGTTTCACATAAATAATGCATATTTTTAAGTCAT FRD3_SHA CCGTTATCATATTATCAGTTTCACATAAATAATGCATATTTTTAAGTCAT FRD3_COL TAAAATATGGAGCCCTCTGCCCTCACTGGCTTTTCTACCCAAATAGAATA FRD3_BAY TAATATATGGAGCCCTCTGCCCTCACTGGCTTTTCTACCCAAATAGAATA FRD3_SHA TAAAATATGGAGCCCTCTGCCCTCACTGGCTTTTCTAACCAAATAGAATA FRD3_COL TTCTGTAT-GAGTATGTTTCAGCTAGACTAGATGGTTCAAAGATATCCGG FRD3_BAY TTCTGTATTGAGTATGTTTTAGCTAGACTAGATAGTTCAAAGATATCCGG FRD3_SHA TTCTGTAT-GAGTATGTTTCAGCTAGACTAGATGGTTCAAAGATATCCGG FRD3_COL TGTCCAAGTGTCGATTTGGTGTTGGTTAAAAAAAATGTAAGAAAAAAAAG FRD3_BAY TGTCCAAGTGTCGATTTGGTGTTGGTTAAAAAAAATGTAAGAAAAAAAAG FRD3_SHA TGTCCAAGTGTCGATTTGGTGTTGGTTAAAAAAA-TGTAAGAAAAAAAAG FRD3_COL CTGGATCTTATCCTTTTACTAAAGAAAATCTGACCACAGTTATTTCCTTA FRD3_BAY CTGGATCTTATCCTTTTACTAAAGAAAATCTGACCATAGTTATTTCCTTA FRD3_SHA CTGGATCTTATCCTTTTACTAAAGAAAATCTGACCATAGTTATTTCCTTA FRD3_COL GTTAAGAGCCAAAATTAGGCTCAGATTTTAATTAATTGAACCATTAGATT FRD3_BAY GTTAAGAGCCAAAATTAGGCTCAGATTTTAATTAATTGAACCATTAGATT FRD3_SHA GTTAAGAGCCAAAATTAGGCTCAGATTTTAATTAATTGAACCATTAGATT FRD3_COL TGTTCTTACTCAATTTCTATGTATTTTAATAAGTTTCACTTCTAAAAGTG FRD3_BAY TGTTCTTACTCAATTTCTATGTATTTTAATAAGTTTCACTTCTAAAAGTG FRD3_SHA TGTTCTTACTCAATTTCTATGTATTTTAATAAGTTTCACTTCTAAAAGTG FRD3_COL TTTTCTAAAGTTTTTCAACTATGTACTTCAAATCTTAAGACTGTTTTTAA FRD3_BAY TTTTCTAAAGTTTTACAACTA----CTTCAAATCTTAAGACTGTTTTTAA FRD3_SHA TTTTCTAAAGTTTTTCAACTATGTACTTCAAATCTTAAGACTGTTTTTAA FRD3_COL TGAATCCAAAATTTACACTTTAATTCAAATTTTCAACTTGTGATTAACAA FRD3_BAY TGAATTCAAAATTTACACTTTAATTCAAATTTTCAACTTGTGATTAACAA FRD3_SHA TGAATCCAAAATTTACACTTTAATTCAAATTTTCAACTTGTGATTAACAA FRD3_COL ATAATTATACTTTATAGGTATTAATTTCCTTGCACTTTTTGGAATG FRD3_BAY ATAATTATACTTTATCGGTATTAATTTCCTTGCACTTTTTGGAATG FRD3_SHA ATAATTATACTTTATAGGTATTAATTTCCTTGCACTTTTTGGA

FRD3_SHA CCGTTTTTGTTGGACTAGGTCTCTACTTTGGTGCCGGAATTTTCTCCAAG

del28-----+

del27-+----

N116SSNNNSS

L117PLLLLLP

in12+++---

genotypeA. lyrata

Bay-0Ita-0Col-0Cvi-0

Shahdara

AtFRD3

Figure S7

B

A

A. t

halia

na

% -

114025186

Figure S8

8

6

4

2

0

10

12

14

16

cDNA mixBay + Sha

cDNABay x ShaF1 plants

Nor

mal

ized

ratio

of B

ay v

s. S

ha a

llele

15

10

5

0

20

25

30A

B

Nor

mal

ized

ratio

of C

t vs.

Ita

alle

le

cDNA mixCt + Ita

cDNACt x Ita

F1 plants

Fe ro

ot c

onte

nt (

mg.

g-1

DW

)

Figure S9

4

5

6

0

0.1

0.2

0.3

0.4

0.5

Bay-

0

NIL

[Sha

]

Shah

dara

NIL

[Bay

]

Col

-0

frd3-

7

50 µm

Bay-0 NIL[Bay] Sha NIL[Sha] Col-0 frd3-7

Figure S10

AtF

RO

2 re

lativ

e tra

nscr

ipt l

evel

ColNIL[Bay ]ShaNIL[Sha]Bay

Figure S11

0

0.5

1.0

1.5

Fe 0 Zn 1 Zn 150

0

1

2

3

4

5

Ferri

c ch

elat

e re

duct

ase

activ

ity (µ

mol

Fe(

II). g

PF.

h)

Bay

-0

NIL

[Sha

]

Sha

hdar

a

NIL

[Bay

]

Col

-0

frd3-

7

+ - + - + - + - + - + - Fe

Figure S12

Citr

ate

conc

entra

tion

in

xyle

m e

xuda

tes

(mM

)0.0

0.1

0.2

0.3

0.4

0.5

0.6

Bay-

0

NIL

[Sha

]

Shah

dara

Col

-0

frd3-

7

Figure S13

b

a

c

a

a