NEUROSERVICE-Corporate_2015

www.neuroservice.com

CORPORATE

PRESENTATION

March, 2015


SUMMARY

OUR

SOLUTIONS

OUR

COMMITMENTS

YOUR

INTERLOCUTORS

WHO

WE ARE

OUR

TECHNIQUES

OUR FOCUS

AREAS


WHO WE ARE

OUR

SOLUTIONS

OUR

COMMITMENTS

YOUR

INTERLOCUTORS

WHO

WE ARE

OUR

TECHNIQUES

OUR FOCUS

AREAS


WHO WE ARE

Core business activity

A Contract Research Organization (CRO) Testing the effect of ANY COMPOUNDS on Central Nervous System tissues


WHAT WE DO

Core business activity

IN VITRO TESTS UNDER PHYSIOLOGICAL CONDITIONS

In rodent (or human) acute brain slices In rodent spinal cord slices In rodent cultured neurons

www.neuroservice.com www.neuroservice.com

WHAT WE DO

Electrophysiological recordings


WHAT WE PROPOSE FOR DRUG DISCOVERY

Your remote laboratory

Hits

Leads

Advanced candidate

IND

LEAD SELECTION &

OPTIMIZATION

MECHANISM OF ACTION

SAFETY & TOXICOLOGY PROFILING

CLINICAL RESEARCH

R&

D

PR

É-C

LIN

ICA

L D

EVEL

OP

MEN

T


WHO WE ARE

Key features

Launched in Aix-en-Provence in 2006

Portfolio of 150 studies

60 worldwide clients (Pfizer, Roche, CHDI…)

35 people – 2 laboratories

Capital: 520 000€

Income 2014 : 2,7 M€


OUR TECHNIQUES

OUR

SOLUTIONS

OUR

COMMITMENTS

YOUR

INTERLOCUTORS

WHO

WE ARE

OUR

TECHNIQUES

OUR FOCUS

AREAS


OUR TECHNIQUES

Multi-Electrode Arrays (MEA) technique


OUR TECHNIQUES

Patch-Clamp technique


OUR TECHNIQUES: THE IDEAL COMBINATION

Patch-Clamp Electrode

MEA Electrode


OUR TECHNIQUES: A COMPREHENSIVE COMBINATION

Different structures and protocols

MATERIAL STRUCTURE PROTOCOL

Input/Output Properties

Paired-Pulse Protocol

Basal Synaptic Transmission

Long-Term Potentiation

Long-Term Depression

Short-Term Potentiation

Firing Activity

Dose-Response Protocol

Spontaneous Postsynaptic Current

Agonist Evoked Postsynaptic Current

Pharmacology of Intracellular Pathways

Hippocampus

Cortex

Cerebellum

Spinal Cord with or without root

Ventral Tegmental Area

Striatum

Substantia Nigra

Locus Coeruleus

Periaqueductal Gray Matter

Dorsal Raphe

Amygdala

Thalamus

Dorsal Vagal Nucleus

i

In vitro rodent slices


OUR TECHNIQUES: THE IDEAL COMBINATION

In vitro evaluation of compounds under the most physiologically relevant conditions Robust and reproducible high quality data

Reducing animal and compound use

Exceptional macroscopic view of neuronal networks Ability to record multiple evoked-responses in parallel within a single slice Mid-throughput production capabilities

Refined investigation of compounds’ effect on single proteins/receptors Large catalog of CNS structures and neuronal cell types to address effects on specific neurons, neurotransmitter systems or receptors

NEURONAL NETWORK SINGLE NEURON

PATCH-CLAMP MEA (Multi-Electrode Arrays))


OUR FOCUS AREAS

OUR

SOLUTIONS

OUR

COMMITMENTS

YOUR

INTERLOCUTORS

WHO

WE ARE

OUR

TECHNIQUES

OUR FOCUS

AREAS Drug Discovery

Safety


OUR FOCUS AREA

LEAD SELECTION &

OPTIMIZATION

MECHANISM OF ACTION


SOLUTIONS

FOR CNS

DRUG

DISCOVERY

SOLUTIONS FOR

SAFETY: Testing the effect of any

type of drug, molecule…

on CNS and investigate its

mechanism of action


SOLUTIONS FOR DRUG DISCOVERY

Example with MEA technique



Example with MEA technique

ACCURATE PHARMACOLOGY

CHOLINERGIC RECEPTOR-MEDIATED INCREASE OF CA1 PYRAMIDAL NEURONS FIRING

CARBACHOL & PIRENZEPINE

M1 MUSCARINIC ACETYLCHOLINE RECEPTORS

A B

C Pirenzepine Carbachol


EXAMPLE WITH MEA TECHNIQUE

Tg Rodent Phenotyping

ELECTROPHYSIOLOGY IN BRAIN SLICES FROM

GENETICALLY ENGINEERED MICE:

Mice deficit (or gain) determination

Physiological characterizations

Drug evaluation in a disease model

FASTER 2 weeks

LESS ANIMALS REQUIRED 10 WT / 10 Tg

… in comparison with behavioural tests

LESS COMPOUNDS REQUIRED



Example with Patch-Clamp

CHOLINERGIC RECEPTOR-MEDIATED INCREASE OF CA1 PYRAMIDAL NEURONS FIRING

CARBACHOL & PIRENZEPINE

EXPERIMENT Miniature inhibitory postsynaptic currents (mIPSCs) in lamina II of adult spinal cord mice

DATA mIPSC frequency, amplitude distribution and detailed analysis of the average mIPSC waveform (including amplitude, rise time, decay time)

100 pA

100 s

Strychnine 1 µM Rat, p15, lamina X TTX, 0.5 µM + ORG 24598, 10 µM, 35°C

dorsal

ventral

II III

10x



Example with Patch-Clamp

PHARMACOLOGICAL CHARACTERIZATION OF SODIUM CURRENTS IN DRG

TTXs and TTXr sodium current n=6

TTXs sodium current n=6

TTXr sodium current n=3

Mice DRG (12 weeks) Rat DRG (12 weeks)

Inactivation Inactivation Inactivation


Human tissue management Protocol design Patch Clamp recording

HUMAN BRAIN SLICE RECORDINGS

A partnership customized to client needs

EXPERT INTERFACE WITH CLIENT

REMOTE LABORATORY

Customer Relationship Management Quotation & Contractual Paperwork Contract and Project Management Raw data validation & Final report

Clients


HUMAN BRAIN SLICE RECORDINGS

STRUCTURE MATERIAL PROTOCOLS

Neurons passive membrane properties

AMPA/kainate “minis” or evoked-currents

NMDA evoked-currents

GABAA “minis” or evoked-currents

… Small areas of cortical brain

tissue

Neurosurgical resections from adult patients For purely medical reasons (informed consent given by patients) Surgery approved by independent Board of Physicians All is supervised by the Ethical Committee from the University of Freiburg (in accordance with both national and international guidelines)


SOLUTIONS FOR SAFETY

Focus areas CUSTOMER WORK FLOW

Advanced candidate

MECHANISM OF ACTION


CLINICAL RESEARCH

Safety Studies

!


OUR SOLUTIONS

OUR

SOLUTIONS

OUR

COMMITMENTS

YOUR

INTERLOCUTORS

WHO

WE ARE

OUR

TECHNIQUES

OUR FOCUS

AREAS


OUR SOLUTIONS

Neuroservice: your remote laboratory

Outsourced Assays R&D

Customized Solutions

Packages

DRIVE OUR RESSOURCES

CUSTOMIZE OUR ASSAYS

CHOOSE A READY TO USE

SOLUTION

Highly experienced team in neurophysiology,

neuropharmacology and neurotoxicology

Portfolio of 150 studies • High quality data

•Short turnaround time •Mid-throughput production

capabilities

4 packages:


OUTSOURCED R&D

Highly skilled scientists

Flexible Multi-Platform FTE Contract


CUSTOMIZED SOLUTIONS

More than 150 studies already provided

Fee for Service Contract

http://www.neuroservice.com/en/our-techniques/


NEUROSAFETY PACKAGE

General profiling on synaptic properties

●

At least one effect detected

! PROPOSAL FOR FURTHER INVESTIGATION


PRO-CONVULSIVE PACKAGE

Prediction of convulsive properties

● At least one effect detected

TEST 1, TEST 2 or TEST 3

TEST 1 TEST 2 TEST 3 OK ● ● ●

! PROPOSAL FOR FURTHER INVESTIGATION


GAMMA OSCILLATIONS PACKAGE

A translational biomarker

Neuronal rhythms are

altered in schizophrenia, epilepsy, autism, ADHD, Alzheimer and Parkinson Diseases.


DRG SODIUM PACKAGE (PAIN)

Effects on DRG Na+ Channels

Sodium channels are involved in pain

associated with neuropathy, diabetes, osteoarthritis and surgery.

(1 mM) (1 mM)


OUR COMMITMENTS

OUR

SOLUTIONS

OUR

COMMITMENTS

YOUR

INTERLOCUTORS

WHO

WE ARE

OUR

TECHNIQUES

OUR FOCUS

AREAS


OUR COMMITMENTS

QUALITY ROBUST AND REPRODUCIBLE DATA

SHORT TURNAROUND 100 VALIDATED SLICES OR 40 NEURONS / WEEK

EXPERTISE INTERACTIVE AND EXPERT SUPPORT

FLEXIBILITY STEP-BY-STEP STUDY PLANS

ETHICS MINIMIZED ANIMAL USE


YOUR INTERLOCUTORS

OUR

SOLUTIONS

OUR

COMMITMENTS

YOUR

INTERLOCUTORS

WHO

WE ARE

OUR

TECHNIQUES

OUR FOCUS

AREAS


YOUR INTERLOCUTORS

BRUNO BUISSON, PhD Founder

General manager &

Chief scientist

SCIENTIFIC TEAM

Based in Japan Based in USA

TATSUO MIYAUCHI, PhD Scientific liaison

JEFFREY SPROUSE, PhD Scientific liaison

OLIVIER TOURY Co-Founder

Deputy general manager &

Chief of business

development

MAI SAITO Asia Business Developer

LINDA HOUAICHI USA & Europe Business Developer

ESTHER STEIDL, MsSc Co-founder

Head of the Multi-Electrode

Array laboratory

BOGDAN SAVA, PhD Head of the Patch-Clamp

laboratory

BUSINESS TEAM

www.neuroservice.com Your partner for hands-on CNS electrophysiology

Domaine de St Hilaire

595, rue Pierre CS 30531

13 593 Aix-en-Provence

Cedex 3

FRANCE

Tel : +33 (0)442 991 220

[email protected]


Documents

NEUROSERVICE-Corporate_2015