P1

1

NACO Lab Technician Training Programme

P2

2

•What do you know about HIV/AIDS?

•Do you feel apprehensive to be involved in HIV Testing?

•What are your expectations from this course?

Introduction

P3

3

What do LTs want to achieve?

•Learn about all aspects HIV/AIDS

•Understand all the various issues and processes/procedures of HIV testing at ICTCs

•Perform laboratory tests in accordance with NACOs HIV testing strategies and testing guidelines

•Understand the interpretation of results

•Ensure standardized and quality testing all across in order to provide accurate, reliable and reproducible test results and meet the requirements of the client

P4

4

Enhance the theoretical and practical skills of LTs with hands on practical sessions and exercises

What do we want to achieve?

•Performance of Rapid HIV tests

•Performance of ELISA assays

•Exercises on:

-collection, storage and transport of samples

-interpretation of results,

- maintenance of equipment,

-practice of quality assurance,

-making of positive control for day to day QC

-inventory management

-quality control

•Exercise on NACO HIV testing strategies

P5

6

What do we want to achieve?

•Reduce stigma within the healthcare personnel - address anxieties and misconceptions to increase willingness to care for HIV positive people

•Prevent occupational exposure to blood borne pathogens by training the healthcare providers in Biosafety, standard work precautions and PEP

•Assure and inform about PEP and its timely use in case of accidental exposure to minimize transmission of HIV

P6

7

Content of Training

Module 1: Introduction

Module 2: Virology and pathogenesis of HIV infection

Module 3: Detection of HIV infection, HIV testing protocols and Strategies

Module 4: Biosafety in the laboratory and standard work precautions

Module 5: Process and flow of the Client at ICTC/PPTCT for HIV counseling and testing

Module 6: Collection, transport and storage of blood sample

Module 7: Elements/components of Total Quality Management/Quality System Essentials

Module 8: Introduction to HIV testing

P7

8

Content of Training

Module 9: Inventory management

Module 10: Document control and various documents

Module 11: Common errors, causes and trouble shooting

Module 12: External quality assessment

Module 13: Equipment maintenance and calibration

Module 14: Performance and characteristics of ELISA, validation, use of Levey Jenning chart

Module 15: Performance and characteristics of HIV rapid tests

Module 16: Preparation of positive control, calculation of range for validation of ELISA for day to day QC

P8

9

Reference material to be provided

•Program agenda

•Hand outs of slides, practical exercises, examples of documents-Temperature log, inventory management, SOP

•NACO lab technician manual

•NACO website: www.nacoonline.org