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Morphological and genetic diversity of the larch populations on the Kuril Island. I.Yu. Adrianova Group of Plant Molecular Genetics Institute of Biology and Soil Sciences, FEB RAS. - PowerPoint PPT Presentation
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Morphological and genetic diversity of the larch populations
on the Kuril Island
I.Yu. Adrianova
Group of Plant Molecular Genetics
Institute of Biology and Soil Sciences, FEB RAS
To study the influence of island isolation on the morphological and genetic diversity of the populations of Larix kamtchatica (Rupr.) Carr. from Iturup Island
The purpose of this research:
Slide 2
The schemes of location of the larch populations studied
- place of the sample collecting Slide 3
•the length of the seed wing
The morphological traits of cones and seeds used in this work
•the length and width of the cone
•the ratio of length to width of the cone
•the deflection angle of scales in the cone
The quantitative trait:
The qualitative trait:
•the shape of the scales •the character of scale edge
•the degree of deflection of the scale edge
• the number of scales in the cone
•the length and width of the seed
Slide 4
Level of variability CV, % Level of variability CV,%
Very low < 7 Higher 21-30
Low 8-12 High 31-40
Medium 13-20 Very high >40
Levels of variability of morphological traits of wood species worked out by S.A. Mamaev
CV – coefficient of variation
Individual variability of all morphological traits ranged from 8 to 21 %.
AMOVA analysis based on all morphological traits doesn’t distinguish these populations (p = 0.204).
Slide 5
Kas_1
Kas_2
Kas_3
Kas_4
Kas_5
Kas_6
Kas_ 7
Kas_8
Khv _1Khv _2
Khv _3
Khv _4
Khv _5
Khv _6 Khv _7
Khv _8Khv _9
Khv _10
Khv _11
Khv _12Khv _13
Khv _14
Khv _15
Khv _16
Khv _17
Khv _18
-7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6
Factor 1: 33 .45%
-5
-4
-3
-2
-1
0
1
2
3
4
5
Fac
tor
2: 2
0.8
6%
PCA ordination of the larch individuals using all morphological traits
Slide 6
Kas- Kasatka
Khv - Khvoinyi
The dendrogram of relationship between trees from two larch populations of Iturup Island based on all
morphological traits of cones and seeds
Ward`s methodEuclidean distances
Kh
v_8
Kh
v_6
Kas
_6
Kh
v_16
Kh
v_13
Kh
v_12
Kh
v_9
Kh
v_7
Kh
v_17
Kas
_2
Kh
v_11
Kh
v_18
Kh
v_5
Kh
v_4
Kh
v_1
Kas
_8
Kas
_7
Kh
v_3
Kas
_5
Kas
_4
Kh
v_10
Kh
v_2
Kh
v_15
Kh
v_14
Kas
_3
Kas
_1
0
100
200
300
400
500
600
Lin
kag
e D
ista
nce
Kas- Kasatka
Khv– Khvoinyi
Slide 7
Slide 8
Sample A P95,% P99,% He I h
Kasatka 1.39 38.9 38.9 0.1339 0.1270 0.19
Khvoinyi 1.55 43.3 50.5 0.1494 0.1507 0.23
The genetic estimations of our populations
A - Observed number of alleles; P95 - % polymorphic loci (95% criterion) ; P99 - % polymorphicloci (99% criterion); He – average expected heterozygosity; I -Shannon's Information index [Lewontin (1972)]; h = Nei's (1973) gene diversity
Larix sibirica Ldb. - A = 1.54; P95 = 45%; He = 0.1698
Larix gmelinii (Rupr.) Rupr. - A= 1.32; P95 = 32%; He = 0.1373
X-sq : 327.6221df : 638Overall: p = 1.0000
Exact tests for population differentiation (Raymond and Rousset, 1995)
Slide 9
UPGMA unrooting tree of the individuals from two larch populations based on Nei’s (1978) genetic distances
Kas- Kasatka
Khv– Khvoinyi
Nm = 5.1
Conclusions:
The island isolation have not caused a decrease of morphological and genetic diversity of the larch populations from Iturup Island
Slide 10
Thank you for your attention !