Following the nucleotide numbering, tape the pieces into a long strip. The blank grey tabs should be hidden. Make slits in-between each double line (16 total). Be careful not to cut through the entire strip. Fold back ("mountain fold") on the horizontal dashed lines Fold in ("valley fold") on the small vertical dotted lines. Bring together bases G19 and C56 (colored yellow) to form a base pair – hide the grey tab by taping it under. This forms the beginnings of the tRNA tertiary structure– the inverted L-shape. To further explore details of tertiary structure see the atomic model of tRNA at bit.ly/HVzIoH Molecular Models: Exploring the Structure of tRNA www.rcsb.org • [email protected] I. The primary structure II. The secondary structure III. The tertiary structure 1. 10-13 with 25-22 2. 27-31 with 43-39 3. 49-53 with 65-61 4. 1-7 with 72-66 Bring colored/patterned sections together, and tape so the grey tab is hidden. It is important that you match the bases in the following order: Notice the almost-perfect base pairing (G:C and A:U bases pair up). At this point the model is a cloverleaf shape– the secondary structure of tRNA. Each colored region represents the double helical regions of the structure. 1 2 3 4 5 6 7 or or or or What is tRNA? Transfer RNA (tRNA) 1 "translates" the genetic code into the language of proteins. Each tRNA molecule binds to a speciﬁc amino acid on the acceptor arm, recognizes its corresponding code on the mRNA through the anticodon loop region and delivers the amino acid to a growing peptide chain in the ribosome 2 for protein synthesis. 1. See the Molecule of the Month feature at doi: 10.2210/rcsb_pdb/mom_2001_3 2. See the Molecule of the Month feature at doi: 10.2210/rcsb_pdb/mom_2010_1 Cut out each piece, leaving the grey tabs intact. bases phosphodiester backbone sugars phosphates nucleotide numbering Acceptor arm D-arm TΨC arm Anticodon arm Anticodon Anticodon Acceptor Instructions for Building the Paper Model of tRNA Acceptor Arm Anticodon Loop Additional information about this activity can be found at: bit.ly/HVzIoH

Molecular Models: Instructions for Building the Paper Model of … · 2017. 8. 24. · model of tRNA at bit.ly/HVzIoH Molecular Models: Exploring the Structure of tRNA • [email protected]

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Page 1: Molecular Models: Instructions for Building the Paper Model of … · 2017. 8. 24. · model of tRNA at bit.ly/HVzIoH Molecular Models: Exploring the Structure of tRNA • info@rcsb.org

Following the nucleotide numbering, tape the pieces into a long strip. The blank grey tabsshould be hidden.

Make slits in-between each double line (16 total).Be careful not to cut through the entire strip.

Fold back ("mountain fold") on the horizontal dashed lines

Fold in ("valley fold") on thesmall vertical dotted lines.

Bring together bases G19 andC56 (colored yellow) to form abase pair – hide the grey tab bytaping it under. This forms thebeginnings of the tRNA tertiarystructure– the inverted L-shape.

To further explore details of tertiary structure see the atomicmodel of tRNA at bit.ly/HVzIoH

Molecular Models: Exploring the Structure of tRNA

www.rcsb.org • [email protected]

I. The primary structure

II. The secondary structure

III. The tertiary structure

1. 10-13 with 25-222. 27-31 with 43-393. 49-53 with 65-614. 1-7 with 72-66

Bring colored/patterned sectionstogether, and tape so the grey tab is hidden. It is importantthat you match the bases in thefollowing order:

Notice the almost-perfect base pairing(G:C and A:U bases pair up). At thispoint the model is a cloverleaf shape–the secondary structure of tRNA. Eachcolored region represents the doublehelical regions of the structure.

1 2 3 4

5 6

orororor

What is tRNA?Transfer RNA (tRNA)1 "translates" the genetic codeinto the language of proteins. Each tRNA moleculebinds to a specific amino acid on the acceptor arm,recognizes its corresponding code on the mRNAthrough the anticodon loop region and deliversthe amino acid to a growing peptide chain in theribosome2 for protein synthesis.

1. See the Molecule of the Month feature at doi:10.2210/rcsb_pdb/mom_2001_3

2. See the Molecule of the Month feature at doi: 10.2210/rcsb_pdb/mom_2010_1

Cut out eachpiece, leavingthe grey tabsintact.

bases

phosphodiester backbone

sugarsphosphates

nucleotidenumbering

Acceptorarm

D-armTΨC arm

Anticodonarm

Anticodon

Acceptor