Module Handbook Biomedical Sciences Master of Science … · · 2018-02-28Module Handbook . Biomedical Sciences . Master of Science ... Fundamentals of Membrane Transport ... Part

Department of Natural Sciences

Module Handbook

Biomedical Sciences Master of Science (M.Sc.)

University of Applied Sciences Bonn-Rhein-Sieg Department of Natural Sciences von-Liebig-Str. 20 53359 Rheinbach www.h-brs.de Version: 2013

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Appendix B: Module Handbook English 2013

Compulsory Courses: Monitoring of Clinical Trials ................................................................................... 3

Pharmacology and Toxicology ................................................................................ 5

Pathophysiology .................................................................................................... 7

Virology ................................................................................................................ 9

Neurobiology ...................................................................................................... 12

Clinical Chemistry ................................................................................................ 14

Medical Proteomics ............................................................................................. 16

Human Genetics .................................................................................................. 18

Advanced and Clinical Immunology (ACI) ............................................................. 20

Final Thesis .......................................................................................................... 22

Elective Courses: Practical Elective: FACS ........................................................................................ 24

Practical Elective: Complement Receptor .............................................................. 26

Practical Elective: Advanced Bioinformatics ........................................................... 27

Practical Elective: Fundamentals of Membrane Transport ...................................... 28

Practical Elective: Pharmacogenetics (PG) ............................................................. 29

Elective Course: Nutrition Physiology .................................................................... 31

Elective Course: Environmental Chemistry and Ecotoxicology ................................ 33

Elective Course: Radiation Biology Basics .............................................................. 35

Elective course: Mammalian genome analysis ....................................................... 37

Elective course: Parasitology ................................................................................. 39

Special Fields in Biology: Introduction to Marketing .............................................. 42

Special Fields in Biology: Environment and Health ............................................... 44

Special Fields in Biology: Free Radicals in Biomedical Research .............................. 47

Special Fields in Biology: Stem Cells ..................................................................... 49

Special Fields in Biology: Physiology of the Skeletal System and Biomaterials ......... 51

Module Handbook: Biomedical Sciences (M.Sc.)

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Module: Monitoring of Clinical Trials

Semester: 1st semester, MSc Biomedical Sciences

Course leader: Prof. Dr. Martin Sieber

Lecturer: Prof. Dr. Martin Sieber & Ann-Marie Lukas

Language: English

Assignment to curriculum:

Compulsory Course, 1st semester MSc Biomedical Sciences

Course units / Hours per week:

Lecture: 2 credit hours

Exercise: 2 credit hours; Group size: 30

Seminar: 2 credit hours; Group size: 15

Student workload: Contact Hours Private Study

Lecture: 30 40

Exercise: 30 50 Seminar: 30 60

Sum: 90 150

Total study hours: 240 hours

Credits: 8 ECTS

Prerequisites according to examination regulations:

none

Recommendations: none

Learning outcomes:

By the end of this lecture/module, the students will: • know the international standards for clinical research,

• be familiar with the regulatory environment for clinical trials,

• be able to identify laws, rules, regulations, essential document requirements and guidelines pertinent to study design, implementation, evaluation and reporting,

• have the means to search for and identify appropriate clinical research literature, identify essential monitoring skills and list key interactions within the study team as part of the clinical trial/research process.

• understand essential marketing aspects for delivery of a new product.

Overall, the students will develop/have a systematic understanding of relevant good clinical practice tools essential for conducting, managing and monitoring clinical trials.


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Summary indicative content:

Lecture Core:

• Methods and protocols, drug and medical device development

• Human subject protection and essentials of Good Clinical Practice

• Ethical committees, informed consent, confidentiality in clinical trials

• Trial protocol, monitoring, organization and record keeping

• Clinical research personnel, site selection, recruitment and retention strategies

• Safety monitoring, adverse events, data management, final report

• Liability, audits and inspections

Group Work: In an evidence-based, collaborative approach, students will be trained to bring together concepts, principles and applications into a pre-defined research project.

Assessment: Individual PowerPoint presentations on a topic exploring specific aspects of clinical research and trial monitoring.

Teaching style: Lectures. Interactive learning, problem-oriented exercises and group work. Slide and notes uploaded to LEA. Guest lectures. “Real world” encounters facilitated via on-site visits.

Indicative Sources: http://www.ema.europa.eu/ema/

http://www.clinicaltrials.gov

http://www.ema.europa.eu/ema/

http://www.clinicaltrials.gov/


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Module: Pharmacology and Toxicology

Semester: 1st semester MSc Biomedical Sciences

Course leader: Prof. Dr. Ulrike Bartz

Lecturer: Prof. Dr. Ulrike Bartz

Language: English

Assignment to curriculum: Compulsory Course 1st semester, MSc Biomedical Sciences




Lab work: 2 credit hours; Group size: 15

Student workload: Contact hours Private study

Lecture: 30 30

Exercise: 30 60 Lab work: 30 60

Sum: 90 150


Credits: 8 ECTS


none


Learning outcomes: Lecture/tutorial/seminar: After successfully completing the module, students are able to work out and understand a new medicinal product (new chemical entity or biological), its dosage form and the relevant clinical picture regarding pharmacokinetic (PK) and pharmacodynamical (PD) properties. This methodology can be directly applied for independently working out further medicinal products relevant in later work life (clinical or biomedical research). Laboratory course: The students are able to analyze (incl. data analysis) and answer questions in the context of PK (urine analysis, toxicology, metabolism) and PD (enzyme based assay).


Lecture: Lectures on drug actions, pharmacokinetics, (L)ADME, pharmacodynamics (mode of action), adverse effects, pharmaceutical interactions, toxicology, in particular: biotransformation, bioactivation, elimination, medical terminology, pharmacokinetic models, cumulation,


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bioavailability, prodrug concept, enterohepatic circulation, pharmacogenetics, preclinical and clinical trials/bioequivalence studies/regulations for drug approval.

Tutorial/seminar: Exercises; in small groups, students have to work on present and dispute about a new medicinal product on the basis of an EPAR (European Product Assessment Report) and a SmPC (Summary of Product Characteristics). Laboratory course: 1. Several experiments in small groups on pharmakokinetics (plasma samples) including the mathematical analysis (excel), one compartment model; oral input, intravenous dose such as or bolus dose or infusion, bolus multiple dose (cumulation); analysis of urinary data, calculation of the relevant pharmacokinetic parameters. 2. Analysis of metabolites (SPE/HPLC or GC/MS of a chosen urine sample) and enzyme based assay (mode of action of a drug).

Assessment: Module with mark Oral examination (70%), lab reports (30%), presentation and discussion sessions: active participation required.

Teaching style: Lecture: PowerPoint, Overhead, blackboard Tutorial/Seminar: exercises, PowerPoint, Overhead, blackboard.

Indicative bibliography/Sources:

- Drug actions - Basic Principles and therapeutic aspects E. Mutschler/H. Derendorf; MedPharm Scientific Publishers (ISBN 3-88763-021-1) - Pharmacokinetic Processes, mathematics and applications Peter G. Welling Wiley Science, newest edition - Applied Biopharmaceutics and Pharmacokinetics L. Shargel/A.Yu; McGraw-Hill Medical Publishing Division; newest edition


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Module: Pathophysiology


Course leader: Prof. Dr. Heinz-Joachim Häbler

Lecturer: Prof. Dr. Heinz-Joachim Häbler

Language: English

Assignment to curriculum: Compulsory module, 1st semester MSc Biomedical Sciences

Course units/Hours per week


Tutorial: 2 credit hours; Group size: 30

Laboratory work: 2 credit hours; Group size: 15

Student work load: Contact hours Private study

Lecture: 30 30

Tutorial: 30 60 Lab work: 30 60

Sum: 90 150

Total study hours: 240

Credits 8 ECTS

Prerequisites according to examinations regulation:

none

Recommendations: Basic knowledge in human or animal physiology acquired in a preceding Bachelor study program

Learning outcomes: Lecture:

• Knowledge of human diseases with a high incidence

• Knowledge of the main principles of general and organ-specific pathophysiology on molecular, cellular and systems levels

• Ability to use the specific pathophysiological terminology

Tutorial: Consolidation of acquired knowledge Laboratory course:

• Transfer of pathophysiological processes to morphological changes of tissue

• Ability to comprehend physiological body functions as a result of a complex interaction between numerous variables and to make predictions about the changes of body functions upon changes of these variables


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Lecture: Basics of pathophysiology, which are necessary for understanding human diseases and their mechanisms. Basic pathophysiological concepts, pathophysiology of the most important organ systems, e.g. the hematopoietic system, the cardiovascular system, the respiratory system, kidneys, gastrointestinal tract, endocrine system.

Tutorial: Recapitulation of subjects discussed in the lectures using appropriate test questions.

Laboratory course: Part 1: Independent analysis using light microscopy of tissue sections showing typical pathological changes.

Part 2: Simulation of the entire cardiovascular and respiratory pathophysiology using computer-teaching programs. Graphical analysis and discussion of results.

Assessment: Module examination – graded Written examination (70% of module grade),

Lab course: Individual report on experiments and measurements (30% of module grade)

Teaching style: Lecture: Overhead, Blackboard

Tutorial: Written list of exercises, Overhead, Blackboard Laboratory course: Computer simulation programs


C.M. Porth, G. Matfin: Pathophysiology, Concepts of Altered Health States, 8th ed., Lippincott Wilkins & Wilkins, 2008

A. Stevens, J.S. Lowe, B. Young: Wheater´s Basic Histopathology, a Colour Atlas and Text, 4th ed., Churchill Livingstone, 2002


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Module: Virology

Semester: 2nd Semester MSc Biomedical Sciences

Course leader: Prof. Dr. Edda Tobiasch

Lecturer: Prof. Dr. Edda Tobiasch

Language: English

Assignment to curriculum: Compulsory Course 2nd Semester MSc Biomedical Sciences


Lecture: 2 credit hours Tutorial: 2 credit hours; Group size: 30


Student workload: Contact hours Private study Lecture: 30 30


Total: 90 150

Total study hours:: 240

Credits: 8 ECTS


None

Recommendations: Previous knowledge in molecular genetics and cell culture from previous study courses

General Safety Instructions for working in laboratories

Safety Instructions for working with S1 and L2 organisms

Learning outcomes: Lecture: At the end of the course, students have the following knowledge:

• Taxonomy as well as structure and replication mechanisms of the most important human pathogenic viruses

• Occurrence and dissemination of viral diseases

• Adaption of the virus to the host: host change, zoonosis, “shift and drift”, evolution

Tutorial:

• Treatment of viral diseases and prevention measurements

• Knowledge how to access and work with specific literature


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Laboratory course:

• Methods of detecting and growing different viral diseases

The students can use these competences to work in the corresponding laboratories in science and development in pharmaceutical industries and universities (e.g. diagnostic laboratories for virus detection).


Lecture: The course focuses on the different viral families with respect to the following aspects: Worldwide infection, the different forms of hepatitis, childhood diseases, prion diseases, viruses and cancer, viruses and gene therapy, “emerging viruses”, viral zoonoses, bio terrorism.

Students are to develop an understanding of human and animal pathogenic viruses with respect to the following issues relevant to medicine and biology: taxonomy, history, morphology of virions, replication strategies (Baltimore classification), transmission, host and risk groups, symptoms, diagnostic methods, protection against the immune system, specific immune reactions, acute and chronic diseases, complications, medication. The cosmopolitan and endemic occurrence of viral diseases is discussed with respect to the following aspects: climatic and social factors of dissemination, epidemiology, eradication measures, and prevention.

Tutorial: The students have to work on their own and present a current scientific paper representing a specific question from virology. In the following discussion the student has to defend this paper and the group has to understand the relation of this paper in the context of the already gained knowledge. Laboratory course: In the practical training, the students will learn to growth viruses and detect them by means of different methods of molecular biology.

Assessment: The module is graded; written exam (100%)

Laboratory course: active participation is verified via individual protocols for the experiments

Teaching style: Lecture: PowerPoint, Overhead, black board Tutorial: paper, PowerPoint, black board

Indicative Fields Virology; D. M. Knipe and P. M. Howley;


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bibliography/Sources: Lippincott Williams & Wilkins

Principles of Viruses Molecular Biology, Pathogenesis and Control; S.J. Flint, L.W. Enquist, R.M. Krug, V.R. Racaniello and A.M. Skalka; ASM Press

Lexikon der Infektionskrankheiten des Menschen; Erreger, Symptome, Diagnose, Therapie und Prophylaxe; G. Darai, M. Handermann, E. Hinz and H.-G. Sonntag; Springer


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Module: Neurobiology

Semester: 2nd semester MSc Biomedical Sciences

Course leaders: Prof. Dr. Heinz-Joachim Häbler / Prof. Dr. Hans Weiher

Lecturers: Prof. Dr. Heinz-Joachim Häbler / Prof. Dr. Hans Weiher

Language: English

Assignment to curriculum: Compulsory module, 2nd Semester MSc Biomedical Sciences.

Course units/Hours per week:

Lectures: 2 credit hours Tutorial: 2 credit hours; Group size: 30


Student work load:: Contact hours Private study Lecture: 30 30

Tutorial: 30 60

Lab work: 30 60 Sum: 90 150


Credits: 8 ECTS


none

Recommendations: Basic knowledge in neuroanatomy und neurophysiology/biophysics from a former BSc (or other basic) curriculum

Learning outcomes: Lectures:

• Detailed knowledge of the functional anatomy of the nervous system.

• Profound knowledge of the basic principles of the function of the cerebral cortex, spinal cord, peripheral nervous system and sensory systems

• Knowledge of modern concepts to explain dysfunctions of the nervous system

Tutorial:

• Consolidation of acquired knowledge, application to concrete problems

Laboratory course:

• Ability to carry out electrophysiological studies on test persons and in computer simulations, and to interpret the results


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• Ability to implement the acquired knowledge and proficiencies in the biomedical field


Lectures: The module covers the function of the central and peripheral nervous system. Subjects are: function of the spinal cord (i.e. reflex mechanisms) and, using selected systems (visual system, memory), the mode of function of the cortex. Integrative aspects between the different levels of hierarchy will be illustrated with respect to the somatosensory and somatomotoric system. Based on the knowledge of the normal function, modern concepts of common diseases of the nervous system are discussed.

Tutorial: Preparation for written examination, student presentations of interesting topics in the field of neurobiology Laboratory course: Independent analysis of selected neurobiological topics (e.g. membrane, muscle) using computer-teaching programs. Practical experiments analyzing reflex systems, EEG and evoked potentials.

Assessment: Module examination – graded Written examination (70% of module grade),

Lab course: Individual report on experiments and measurements (30% of module grade)

Teaching style: Lecture: Overhead, Blackboard

Tutorial: Written list of exercises, Overhead, Blackboard


E.R. Kandel, J.H. Schwartz, T.M. Jessell, S.A. Siegelbaum, A.J. Hudspeth: Principles of Neural Science, 5th ed., McGraw Hill, 2012 D. Purves, G.J. Augustine, D. Fitzpatrick, W.C. Hall, A.-S. LaMantia, L.E. White: Neuroscience, 5th ed., Sinauer, 2012


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Module: Clinical Chemistry


Course leader: Dr. Petra B. Musholt

Lecturer: Dr. Petra B. Musholt

Language: English

Assignment to curriculum: Compulsory course in 2nd semester MSc Biomedical Sciences


Lectures: 2 credit hours

Tutorial: 2 credit hours; Group size: 30



Lecture: 30 30


Sum: 90 150


Credits: 8 ECTS


none


Learning outcomes: After completing this module students are able to read and evaluate laboratory result sheets of routine laboratories and to interpret and question potential errors / limitations occurring during (pre-)analysis. They know exemplary physiologic and pathological alterations of main analytes of human organs as well as typical laboratory results of selected relevant human diseases, the basics of (pre-)analytics and how to interpret and statistically quality control the results of analyses derived from human matrices such as whole blood, plasma, serum, and urine.


Lecture/Seminar: In the seminar they have learned how to work out the most widely used Clinical Chemistry analytical methods such as laboratory automation, (immuno-) turbidometry, photospectrometry, nephelometry, EIA, enzyme-activity-assays, iso-enzyme quantitation, serum electrophoresis, urine microscopy, HPLC, real-time PCR, modern sequencing etc., and presented their findings to the fellows in an oral presentation. Basics and concepts in Clinical Chemistry and


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laboratory analytical methods as well as anatomical / medical basic principles influencing laboratory main analytes, as measured from human matrices, will be communicated.

Practical course: The students will apply some of the analytical methods learned in the theoretical part, especially via use of point-of-care testing systems.

Assessment: Module exam (hand-out on a selected clinical-chemistry analytical method) with marks. Written exam (100%)

Practical Course: active participation is required and documented.

Teaching style: Lectures/Seminar: PowerPoint, Overhead, and Board. Seminar: written report, student oral presentation via PowerPoint


Tietz Fundamentals of Clinical Chemistry, Burtis, Aswood, Bruns; publisher: Saunders; latest edition

The Science of Laboratory Diagnosis, Burnett; publisher: John Wiley & sons, latest edition


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Module: Medical Proteomics

Semester: 3rd Semester MSc Biomedical Sciences

Course Leader: Prof. Dr. Angelika Muscate-Magnussen

Lecturer: NN

Language: English

Assignment in Curriculum Compulsory Course in 3rd Semester MSc Biomedical Sciences

Course Units/Credit hours Lecture: 2 credit hours



Students workload: Contact hours Private study

Lecture: 30 30


Sum: 90 150


Credits 8 ECTS


none

Recommendations: Module Biochemistry

Learning outcomes: After successfully completing the course, students are able to explain and apply the following methods and perform the following procedures:

• Protein separation

• Mass spectrometry analysis of proteins and peptides

• Peptide analysis

• Identification and quantification of proteins from cells and biological fluids

• Analysis of postranslational modifications and protein-protein interactions

Summary indicative content: Lecture/Exercise:

Preparation of samples: general properties, sampling, methods of cell disruption, handling of protein samples, protein digestion

Separation methods for proteome analytics: principles of the 1D and 2D SDS-PAGE, preparative IEF, capillary electrophoresis, capillary gel electrophoresis and capillary isoelectrical focusing, HPLC (reversed phase, ion exchange, size exclusion chromatography), multidimensional LC, LC-MALDI


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Methods in proteome analytics based on mass spectrometry: basics, MALDI-TOF-MS, MALDI-TOF/TOF-MS, ESI-MS and ESI-MS/MS, analyzers (ion trap, quadrupole, TOF), sequencing of peptides, SELDI, protein databases and search algorithms Applications: mining, peptide mass fingerprinting, 2D SDS PAGE linked with MALDI-TOF, LC-ESI-MS/MS, MALDI-TOF/TOF-MS; expression profiling, comparative 2D SDS PAGE analysis, isotope markers, protein-protein interactions, immune precipitation, Yeast-Two-Hybrid System, Shot Gun approach, Bait/Reverse Bait, posttranslational modifications

Protein separation: affinity, hydrophobicity, gel filtration and ion exchange chromatography, ultrafiltration, protein precipitation, determination of purity, protein characterization Laboratory Course: Purification and determination of the specific activity of a protein, which is, expressed in E. Coli cells. 2D-gel analysis of a protein in induced and uninduced E. coli cells. Peptide cleavage of lysozyme with trypsin and analysis of the digested peptides by reverse phase HPLC.

Assessment: Module exam - graded

Written examination: 50%; project report: 20%; lab report: 30%

Teaching style: The module “Medical Proteomics” consists of:

1. a weekly two-hour lecture on proteomics

2. a weekly two-hour seminar for consolidation and application purposes; review of the weekly homework; preparation for a project in the field of proteomics 3. a five-day intensive practical course, use of current methods in the field of proteomics, report writing

4. an excursion to a company working in the field of proteomics

5. a two-hour written examination

Indicative Bibliography/Sources:

Daniel C. Liebler, Introduction to Proteomics, Humana Press, 2002

Robert H. Abeles et al., Biochemistry, Jones und Bartlett Publishers, 1992

Hubert Rehm, Proteinbiochemie/Proteomics, Spektrum Akademischer Verlag Heidelberg Michael Kinter und Nicholas E. Sherman, Protein Sequencing and Identification using Tandem Mass Spectrometry, Wiley-Interscience, 2000


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Module: Human Genetics


Course Leader: Prof. Dr. Hans Weiher

Lecturer: Prof. Dr. Hans Weiher

Language: English

Assignment in Curriculum Compulsory Course in 3rd Semester MSc Biomedical Sciences




Students workload: Contact hours Private study

Lecture: 30 30


Sum: 90 150


Credits 8 ECTS


none

Recommendations: Basic knowledge in Molecular Biology/Genetics from a former BSc (or other basic) curriculum

Learning outcomes: At the end of this unit the students are able to:

1. identify human gene variations using methods of molecular genetic analysis

2. apply hybridization techniques in tumor diagnosis 3. analyze food products for their genetic properties

4. employ chromosome analyses in prenatal and tumor diagnosis 5. carry out genetic analyses in forensic applications

Summary indicative content: Lecture/Seminar: Mendelian Genetics, extensions to and exceptions from Mendelian Genetics, sexual development , sex chromosomes, X-chromosome inactivation, influence of sex on genetic properties, genomic imprinting, multifactorial traits, behavioral genetics, population genetics, human evolution, genetics of human cancer, gene therapy and genetic consulting, reproductive technologies, cloning of mammals, ethical issues, selected topics of human genetic research.


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Practical course: Cytogenetic and other human genetic analysis methods, karyotype analyses, identification of sex chromosomes, fluorescence in situ hybridization (FISH).

Analysis of karyotypes in unknown genetic and tumor samples. Experimental analysis of genetic polymorphisms.

Assessment: Module exam – graded

Written test: 50%; written practical course report 50%

Teaching style: The teaching unit consists of a lecture and exercises, in which either specific questions are addressed and discussed, or specific issues are presented by the students. In addition, the practical course offers the opportunity gain hands on experience on the techniques of human genetic analysis. The students produce a report on the results of the laboratory experiments. At the end of the course there is a written examination. Media used are black board and PowerPoint.


Human Genetics by Ricky Lewis, Mc Graw Hill, 2006 (lecture) Human Cytogenetics. A Practical Approach. Rooney DE, Czepulkowski BH; Eds. IRL Press Oxford 1992

Human chromosomes. Manual of basic techniques. Verma RS, Babu A, Pergamon Press New York 1989

Human chromosomes. Miller OJ, Therman E, 4th ed. Springer, New York, 2001

ISCN 1995 - An International System for Human Cytogenetic Nomenclature. Mitelman F, Karger, Basel 1995 In situ hybridization. Principles and Practice. Polak JM, McGee JO´D, Eds., Oxford University Press, Oxford 1990

In situ hybridization protocols. Methods in Molecular Biology. Andy Choo KH, eds, Humana Press, Totowa 1994

In situ Hybridisierung. Leitch AR, Schwarzacher AR, Jackson D, Leitch IJ, Spektrum 1994


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Module: Advanced and Clinical Immunology (ACI)

Semester: 3rd semester MSc in Biomedical Sciences

Course leader: Prof. Dr. Harald Illges

Lecturer: Prof. Dr. Harald Illges

Language: English

Assignment to curriculum: Compulsory course, 3rd semester MSc in Biomedical Sciences



Seminar: 2 credit hours; Group size: 30 Laboratory work: 2 credit hours; Group size: 15


Lecture: 30 30 Tutorial: 30 60

Lab work: 30 60

Total: 90 150 Total study hours: 240 hours

Credits: 8 ECTS


None

Recommendations: None

Learning outcomes: Lecture: The students will have a comprehensive overview about the immune system and some immunological diseases. In particular they can define immunodeficiencies like SCID, X-linked immunological diseases, AIDS, allergies and related genetics.

Seminar: The students will be able to work with “model systems” to analyze diseases. To know how to write grants and understand the basics of the finances required for.

Laboratory course: The students know important immunological techniques and their application like FACS, ELISA, analysis of gene inheritance and a locomotion test in laboratory animals.


Lecture: The lecture will teach the students the fundamentals in cellular (development of lymphocytes in the bone marrow and thymus, differentiation and activation of


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leucocytes, leucocytes during immune responses) and molecular immunology (signal cascades, development of BCR and TCR, marker genes, homing). They will learn the basics of experimental work (relevant technologies e.g. knock-out, knock-in, conditional knock-out, regulatory cells, antibody and recombinant immunological molecules for therapies and experimental approaches. They will learn the basis of important immunological diseases

Seminar: In the seminar we will either present and discuss the scientific literature about an immunological mouse model, or prepare grants about a defined subject. Laboratory course: The practical part concerns experimental work based on the theoretical part from lecture and seminar

Assessment: Module examination - graded

Written exam 60%. Protocol practical course 30%, grant/presentation 10%

Teaching style: According to need.


Janeway Immunobiology, Garland Science, last edition


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Module: Final Thesis

Semester: 4th Semester MSc Biomedical Sciences

Course Leader: Course leaders of the department

Lecturer: Course leaders of the department

Language: English

Assignment in Curriculum Compulsory course in 4th Sem. MSc Biomedical Sciences

Course Units/Credit hours The Master thesis is done either in research groups of the department or in national or international research groups, which offer research activities which match the focus of the study program. During the master thesis, the students are supervised by at least one professor from the department, who also evaluates the final thesis. Details can be found in the examination regulations.

Students workload: Contact hours: 17,5 weeks, with a work load of 40 hours/week Private study (writing of thesis, preparing of oral presentation, learning for final exam): 5 weeks, with a work load of 40 hours/week

Total Sum: 900 hours

Credits 30 ECTS


Admission to Master thesis, if not more than two compulsory courses have not been passed.

Admission to the Master thesis is regulated under §14 in the Examination Regulations.


Learning outcomes: The students are able to solve independently and in a given time complex scientific questions in their special fields of work. They are also able to present their results both literally and orally in an adequate manner. The Master thesis demonstrates the ability for independent scientific work, and the competence to use theoretical and analytical abilities for the solution of specific scientific questions. It also reveals social competence as well as the ability to solve complex problems.


Theoretical and practical work to solve research-related questions using scientific methods. Practical application of knowledge and skills gained during the Master studies, and their accentuation in specific topics. The results have to be summarized in a scientific document, i.e. the Master thesis. The students present their results in a defined time frame, and defend their results in a final oral examination.


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Assessment: Master-Thesis: marked Oral examination: marked

Teaching style: According to need.


According to need.


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Elective courses Module: Practical Elective: FACS




Language: English

Assignment to curriculum: Practical elective, 1st semester MSc Biomedical Sciences


Laboratory work: 6 credit hours

Student workload: Contact hours Private study Lab work: 90 90


Credits: 6 ECTS


none


Learning outcomes: Laboratory course: The students are able to

• perform FACS measurements independently

• take care for the FACS machine (basics)

• to evaluate and organize FACS data

• perform statistical evaluation of FACS data


Laboratory course: The students will be introduced to the theoretical basis of the FACS technology. They will understand the basis of the FACS machines and technology and the used principles of fluidics, optics/lasers and electronics, understand to run the machines and apply the CellQuest program (used for setup and steering the machine), calibration, recording of data, multiparameter analysis of 4 color experiments statistical evaluation of single and multicolor stained cell, dotplot and histogram evaluation of data, DNA/cell cycle experiments.

Assessment: Active participation demonstrated by protocol

Teaching style: Combination of theory and practical work in the laboratory


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Handbook of flow cytometry methods, Robinson (Editor) A guide to Fluorescent Probes and Labeling Technologies, 10. Edition, Molecular Probes


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Module: Practical Elective: Complement Receptor

Semester: 1st semester MSc in Biomedical Sciences



Language: English

Assignment to curriculum: Practical elective, 1st MSc in Biomedical Sciences




Lab work: 90 90 Total study hours: 180 hours

Credits: 6 ECTS


None


Learning outcomes: The students are able to explain the immunology and molecular biology of complement receptors. They understand shedding of membrane bound molecules, experimental techniques to analyze CD21 based on actual literature. The techniques know are tissue culture, ELISA, FCAS and molecular biology.


Laboratory course: Basics of complement receptor biology/immunology. Redox regulation of shedding, current research questions about CD21-

Assessment: Active participation demonstrated by protocol

Teaching style: Combination of theory and practical work in the laboratory


Current scientific literature


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Module: Practical Elective: Advanced Bioinformatics

Semester: 1st Semester MSc Biomedical Sciences

Course leader: Dr. Kurt Stüber

dito Dr. Kurt Stüber

Language: English


Practical Elective 1st Semester MSc Biomedical Sciences




Lab work: 90 90


Credits: 6 ECTS


None


Learning outcomes: The students are able to perform data base searches, sequence comparisons, pattern recognition, evolutionary tree construction, gene prediction, prediction of secondary and tertiary structure of proteins and nucleic acids.

They are familiar with and know secondary data bases (for instance gene ontology, biochemical pathways and taxonomical data).


The course will take place in one of the computer rooms of the institutes. Students have access to the internet and are able to use preinstalled software on the institute servers. Only non-commercial free software will be used.

Assessment: The course is not graded. There is a written exam at the end of the semester.

Teaching style: PowerPoint presentations, live computer demonstrations, blackboard.


Introduction to Bioinformatics. Arthur M. Lesk (2006) Oxford University Press or comparable text books


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Module: Practical Elective: Fundamentals of Membrane Transport

Semester: 1. Semester MSc Biomedical Sciences

Course leader: Prof. Dr. Christopher Volk

Lecturer: Prof. Dr. Christopher Volk

Language: English

Assignment to curriculum: Practical Elective 1st Semester MSc Biomedical Sciences





Credits: 6 ECTS


none


Learning outcomes: At the end of the course the students:

• understand the principle mechanisms of membrane transport and recognize the different types of transporters and channels

• are able to apply oocytes of Xenopus laevis as a tool for the expression of heterologous proteins

• are able to perform measurements of membrane potentials and membrane currents using a two-electrode voltage-clamp system


The students will inject RNA of membrane transport proteins into Xenopus oocytes to express the proteins. Subsequently, they will use these oocytes to perform voltage-clamp measurements of substrate-induced currents and analyze the obtained data.

In an accompanying seminar the theoretical background will be presented by the lecturer and short presentations of scientific papers will be given by the students.

Assessment: The mode of examination is announced by the beginning of the semester. No grading in this course.

Teaching style: The unit consists of a 6 SWS practical course, including an accompanying theoretical seminar.


The Axon Guide http://www.culturacientifica.org/textosudc/Axon_Guide.pdf


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Module: Practical Elective: Pharmacogenetics (PG)


Course leader: Prof. Dr. Weisshaar

Lecturer: Prof. Dr. Weisshaar

Language: English

Assignment to curriculum: Elective Course in 1st Semester MSc Biomedical Sciences


Laboratory work: 6 credit hours, Group size 6



Credits: 6 ECTS


none

Recommendations: Bachelor Biochemistry and Molecular Biology courses

Learning outcomes: • The students can apply the genotyping analysis using Real-time PCR and microarray technology.

• They can isolate DNA from Blood samples for genetic analysis.

• They are able to carry out and interpret Real-time PCR using a Real-time capillary system

• They are able to use a genopChip and perform a microarray analysis using the Nutrigenomic Software


The students will analyze the roll of different mutations in genes which are linked to nutrigenetics. For this purpose they will analyze different probes of DNA for the occurrence of predispositions of food intolerance. The project is in directly linked to a commercial project. The practical task involves the analysis of the gen variant LCT-13910 for lactose intolerance by PCR.

In addition the students will analyze a wide variety of genetic-related food intolerances using the GenoChip (DNA macroarrary).

Assessment: Active participation demonstrated by lab report und oral presentation

Indicative Laboratory Scripts:


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bibliography/Sources: • Laboratory experiments in Pharmacogenetics

• Pharmacogenomics: the inherited basis for the interindividual differences in drug response. Ann.Rev.Genomics Hum- Genet. 2001,2: 9-39

The literature for the seminar is given to the students at the beginning of the course.


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Module: Elective Course: Nutrition Physiology


Course leader: Dr. Langhoff

Lecturer: Dr. Langhoff

Language: English

Assignment to curriculum: Elective course 2nd Semester MSc Biomedical Sciences



Tutorial: 2 credit hours Laboratory work: 2 credit hours



Lab work: 30 30


Credits: 6 ECTS


None


Learning outcomes: Lecture: The students are able to recognize the metabolic context of nutrients

• depending on health and activity status

• in sports

• in important diseases

Students understand the theory of high pressure homogenization and are able to prepare nutrient emulsions.

Lab course: Students are able to prepare simple recipes and are familiar with the basics of homogenization.

Summary indicative content Lecture: Metabolic and biochemistry Pathways in Nutrition. New Facts in Nutrtion Science. Biochemistry, pharmacology and toxicology of Carbohydrates, Proteins and fats.

Thermodynamic: 1. and 2.Law. Nano-effects: Noyes Whitney and Ostwald-Freundlich Equation.


32

Transdermal Systems, Joule Thomson Effect.

Tutorial: Presentation of a publication.

Laboratory course: Active participation with protocols.

Assessment: Written examination – not graded

Laboratory work: Active participation demonstrated by laboratory reports

Teaching style: PowerPoint, blackboard.


Tortora, Principles of Anatomy and Physiology 12th Edition Stryer, Biochemistry 5th Edition.

Atkins, Physical Chemistry 9th Edition


33

Module: Elective Course: Environmental Chemistry and Ecotoxicology

Semester: 2. Semester Master Biomedical Sciences

Course leader: Prof. Dr. Gerd Knupp

Lecturer: Prof. Dr. Gerd Knupp

Language: English

Assignment to curriculum: Elective Course 2. Semester MSc Biomedical Sciences


V: 2 SWS S: 2 SWS; group size: 30

P: 2 SWS; group size: 10

Student workload: Contact hours Private study Lecture: 30 hours 30 hours

Tutorial: 30 hours 30 hours

Lab work: 30 hours 30 hours Total: 90 hours 90 hours


Credits: 6 ECTS


none


Learning outcomes: The students understand the chemistry of the most important environmental compounds. They know the basics of the chemistry of the environmental compartments air, water and soil. They understand ecotoxicology as a modern multidiciplinary natural science and the impact of chemicals on the biology of the ecosystem. They are able to evaluate basic environmental concepts in the context of actual research questions and do know how to address these questions in laboratory work. They are able to design strategies to analyse the impact of human-related changes in the ecological system and to prevent dangerous impact on the ecosystem thereof.

Summary indicative content Lecture: basic concepts of environmental chemistry, major classes and properties of important environmental chemicals, pollutants and toxins, chemistry of the air, the soil and of water; description of an ecosystem, fundamentals of ecotoxicology, the fate of pollutants in ecosystems, analytical methods in environmental


34

chemistry and ecotoxicology, toxicity testing, ecological risk assessment Exercise: Calculating ADI-values from NOEL, statistical evaluation of environmental data, atomic economy calculation; student´s oral presentation of an environmental or ecotoxicologic topic of choice Laboratory Course: GC/MS-analysis of VOCs from synthetic polymers; HPLC-analysis of PAHs in soil; determination of important parameters for water and waste water control (e.g.: P, N, COD, BOD, AOX, TOC ), heavy metals in sewage sludge, experimental design for a toxicity test for the estimation of acute toxicity of a heavy metal loaded water (e.g. OECD terrestrial plants growth test), visiting a waste water treatment plant

Assessment: Oral examination and course work. The mode of examination is announced by the beginning of the semester. No grading in this course.

Teaching style: PowerPoint, blackboard


Baird, C., Cann, M. Environmental Chemistry, W. H. Freemann and Company, New York, 3rd ed., 2005 Wayne G.L., Ming-Ho Y., Introduction to Environmental Toxicology, CRC Press, 2004 Newman, M.C. et al., Fundamentals of Ecotoxicology,CRC Press, 2002

Fent K., Ökotoxikologie, Thieme, Stuttgart, 2003


35

Module: Elective Course: Radiation Biology Basics


Course leader: Dr. Christa Baumstark-Khan

Lecturer: Dr. Christa Baumstark-Khan, PD Dr. Christine Hellweg, Dr. Thomas Berger

Language: English

Assignment to curriculum: Elective Course in 2nd Semester MSc Biomedical Sciences


Lecture: 1 credit hour

Tutorial: 1 credit hour Laboratory work: 1 credit hour



Lab work: 15 15


Credits: 3 ECTS


none


Learning outcomes: Lecture and Tutorial: After successfully completing the module, students have an overview on radiation biology: from physical interactions of radiation with matter, radiation chemistry, radiation effects on cells, tissues and organs as well as on the whole organism. They are familiar with medical aspects of radiation biology, such as radiation diagnostics, tumor therapy and radiation protection. Laboratory course: After completing the experimental part, the students have an up-to-date overview on experimental methods used in radiobiological science fort the understanding of cellular radiation effects for the benefit of patients and radiation workers.


The module consists of lectures, seminars and experimental work. Seminars are complemented by individual student presentations on selected topics in the field of radiation biology and by group


36

presentations on the experimental work. An excursion to a medical radiation facility is also included. The mode of examination is announced by the beginning of the semester.

Lecture: Natural and man-made sources of radiation, interactions of radiation with matter, radiation chemistry, DNA damage, the cytosol and radiation response, characteristics of cell survival curves, use of radiation for cancer therapy, low dose effects on humans, whole body irradiation, lessons from Hiroshima, Nagasaki and Chernobyl, radiation protection. Tutorial: Use of radiation for age determination in archaeology, air crew radiation exposure, radiation accidents, high background radiation areas, astronauts organ doses, radiation chemistry, Deinococcus radiodurans, sterilization of mosquitos, biosensors, solar radiation consequences, DNA damage analysis, DNA strand breaks, cancer stem cells and radiation therapy, bystander effect.

Laboratory course: Detectors in radiation physics, space radiation, X-ray dosimetry, chemical dosimetry, cell survival after irradiation, radiation-induced cell cycle perturbations, visualization of DNA damage, biosensors for genotoxicity

Assessment: Module is not graded. A written final exam about the content of the lecture.

Active participation in the laboratory course, demonstrated by lab reports.

Teaching style: Lecture: PowerPoint, blackboard Tutorial: PowerPoint, blackboard


Radiobiology for the Radiologist by E.J. Hall and A.J. Giaccia, 7th Edition, Wolters/Kluwer

Basic Clinical Radiobiology by M. Joiner and A. van der Kogel, 4th Edition, Macmillan Publishing

Klinische Strahlenbiologie, kurz und bündig by T. Herrmann, M. Baumann and W. Dörr, 4. Auflage, Urban und Fischer


37

Module: Elective course: Mammalian genome analysis


Course Leader: Prof. Dr. Hans Weiher

Lecturer: Prof. Dr. Hans Weiher

Language: English

Assignment in Curriculum Elective Course in 2nd Semester MSc Biomedical Sciences


Tutorial: 2 credit hoursPractical course: 2 credit hours

Students workload: Contact hours Private study L: 30 30

T: 30 30

P: 30 30 Sum: 90 90


Credits 6 ECTS


None


Learning outcomes: After finishing this unit the students are able to:

• prepare DNA samples from mammalian tissue for genetic analyses

• carry out and interpret PCR genetic analyses of mammalian samples.

• design and prepare gene constructs for specific inhibition of gene expression in mammalian cells, so called knock down constructs

Summary indicative content: The students purify DNA from murine tissue samples. They then carry out a PCR analysis with respect to a disease relevant genetic polymorphism. Furthermore, in the context of investigating the underlying pathomechanisms, so called “knock down” gene constructs are prepared, which in future experiments will be used to inhibit the expression of several candidate genes, supposedly involved in the disease development. The theoretic background of the work is subject of seminars, held in parallel, in which the students present the corresponding original papers.

Assessment: The mode of examination is announced at the beginning of the semester. No grading in this course.

Teaching style: PowerPoint, blackboard


38


- Sambrook J., Russell D.W. Molecular Cloning (2001)

- selected original publications on the issue - laboratory protocols


39

Module: Elective course: Parasitology

Semester: 2nd Semester Master

Course leader: Prof. Dr. Dieter Reinscheid

Lecturer: Prof. Dr. Dieter Reinscheid

Language: English

Assignment to curriculum: Special Field in 2nd Semester MSc Biomedical Sciences



Tutorial: 1 credit hours

Student workload: Contact hours Private study Lecture: 30 30

Tutorial: 15 15


Credits: 3 ECTS


none


Learning outcomes: Lecture: At the end of the lecture, the students are able

• to obtain and evaluate epidemiological data about the

• origin and spread of parasitic infections

• to name typical symptoms of parasitic infections

• to name the mode of transmission for different parasites

• to describe the life cycles of different parasites and their use for therapeutic approaches

• to know specific metabolic pathways of parasites

• to describe defense mechanisms of parasites to escape from the immune system

Tutorial: At the end of the tutorial, the students are able

• to develop prophylactic measurements against parasitic infections


40

• to develop therapeutic strategies against parasitic infections

• to develop strategies to control parasites and their vectors

• to diagnose parasitic infections according to typical symptoms


Lecture: Understanding human and animal parasites in respect to the following medical aspects:

• Structures involved in host attachment, damage to host tissue or penetration of host barriers;

• Structure and function of species-specific organelles or organs;

• Protection from the immune system;

• Origin of epidemic or endemic spread of parasitic infections;

• Socioeconomic consequences of parasitic infections;

• Environmental resistant forms;

• Developmental and larval stages;

• Anamnesis, Diagnostic techniques;

• Drug and surgical treatment;

• Strategies for the control of parasites and their vectors.

Tutorial: Questions about the content of the lecture, which requires deep reflection of the study content, internet or literature searches. Discussion of the answers to the questions in the study group.

Assessment: Assessment – non graded

Written exam about the content of the lecture and tutorial

Laboratory Course: Active contribution verified by individual laboratory report

Teaching style: Lecture: PowerPoint, Blackboard

Tutorial: Written exercise questions, Blackboard


Diagnostic Medical Parasitology, LS Garcia, American Society for Microbiology Press, Washington, 2006

Foundations of Parasitology, LS Roberts, J Janovy, S Nadler, McGraw Hill Higher Education, Boston, 2012

Practical Exercises in Parasitology, DW Halton,


41

JM Behnke and I Marschall, Cambridge University Press, 2005 Human Parasitology, BJ Bogitsh, CE Carter, TN Oeltmann, Academic Press, 2012


42

Module: Special Fields in Biology: Introduction to Marketing


Course leader: M.S.c., Dipl.-Kauf. (FH) Simone Fritzen

Lecturer: M.S.c., Dipl.-Kauf. (FH) Simone Fritzen

Language: English

Assignment to curriculum: Special Fields 3rd Semester Master Biomedical Sciences


Lecture: 3 SWS


Lecture: 30 30

Tutorial: 15 15 Total 45 45


Credits: 3 ECTS


None


Learning outcomes: Lecture: At the end of the course the students know:

• The importance of marketing for the company

• The importance of market research and methods for data acquisition and analysis

• Methods for detecting trends

• The elements of the marketing mix and how to use them for:

- Product policy, especially brand policy

- Price policy

- Communication policy - Distribution policy

• Simple models for explaining buying behavior

• Customer relationship management, pros and cons

• Basics of neuromarketing and the influence of this theory on marketing

Tutorial: The students are able to apply the topics of the lecture in practical case studies.


43


Lecture: Market research, data acquisition, hypothesis formulation and testing; product policy; brand policy; price policy; communication policy; distribution policy; psychic determinates and environmental determinates of consumer behavior; the SR-Model; the SOR-Model; the Engel/Kollat/Blackwell model; the Howard/Sheth model; customer relationship management; memory, remembrance and learning; types of perception according to Heath and the consequences for promotion development; brain corresponding communication

Tutorial: Case studies; development, execution and analysis of simple neuromarketing experiments; expert lectures; excursions generating practical knowledge and inside into business day to day work

Assessment: Module exam – graded Written final exam (100%)

Tutorial: Active participation in excursions, written individual reports

Teaching style: Lecture: PowerPoint, Overhead, Black Board Course: Exercise compilation, Overhead, Black Board


Kumar et al.: Essentials of Marketing Research Kotler; Armstrong: Principles of Marketing

Kroeber-Riel; Weinberg: Konsumentenverhalten

Raab et al.: Neuromarketing


44

Module: Special Fields in Biology: Environment and Health


Course Leader: Prof. Dr. G. Klein

Lecturer: Prof. Dr. G. Klein

Language English

Assignment in Curriculum Special Fields 3rd Semester, MSc Biomedical Sciences

Course Units/Credit hours: Lecture: 2 credit hours Exercise: 1 credit hours

Students workload: Contact hours Private study Lecture: 30 30

Exercise: 15 15

Sum: 45 45 Total study hours: 90 hours

Learning outcomes: At the end of the course the students

• can give an overview about historical and political processes at the environment-health interface,

• understand in-depth the sustainable or non-sustainable human- environment interactions,

• understand effective means and tools for creating environments

• favorable to human health,

• have an in depth knowledge about the impact of industrial and societal activities on human well-being,

• know the direct or indirect economicsequences of violating basic natural law,

• understand the basics of natural resources and global systems, and limits to their use or exploitation.


45

Content: This course will describe the links between environmental conditions and human health and well-being. The approach towards creating environments supportive to human Health and Wellbeing goes well beyond the idea of protecting natural environments in their own value, and moves the agenda from a protective and charitable attitude towards a creative and sustainable investment strategy on several fields of action:

• The science-policy process from "Limits to growth" (1972) across the Rio-Earth Summit (1992) towards Agenda 21in Policy, and practical Implementation in presence and future, towards achieving sustainable Human Health and Well-being,

• Environmental Health (EH) Policy as effective tool in Health Promotion and Disease Prevention.

• Policy areas relevant for EH, constructive processes at national, regional and global level.

• Case studies in fields like Transport, Water and Energy Management, Agriculture and Food Production.

• Environment and Lifestyle, with special attention to Water and Air

• Hygiene, Food and Tobacco Smoking.

• General aspects of EH-Economy and use of natural Resources.

• Cultural aspects, Human Rights, Respect and Dignity, harmonizing

• Ecology and Economy. Each student will elaborate on one of the thematic areas and present it as power point presentation in the group for in depth discussion. This presentation will form the basis for the final presentation, which forms part of the final exam.

Examination: At the end of the course a final oral test will be taken in a power point presentation. The overall credits will be added as

• the oral test (75%)

• active participation in the seminar (25%)


46

Literature: Meadows: Limits to Growth, W.v.Dieren: Taking Nature into Account, WHO: EH in Europe; EEA: Europe’s Environment, Late lessons from early warnings; Wuppertal Institut: Nachhaltiges Deutschland (Literature collection available on LEA)


47

Module: Special Fields in Biology: Free Radicals in Biomedical Research

Semester: 3rd semester, Master in Biomedical Sciences

Course leader: Dr. Nadina Stadler

Lecturer: Dr. Nadina Stadler

Language: English


Special Field in 3rd Semester MSc Biomedical Sciences



Exercises: 1 credit hours

Student workload: Contact Hours Private Study

Lecture: 30 30

Exercises: 15 15 Total: 45 45


Credits: 3 ECTS


None


Learning outcomes: Students understand the multiple roles played by oxidative stress/free radicals in the etiology and pathogenesis of cardiovascular, neurodegenerative diseases, and cancer. They will be able to identify and critically review current biomarkers of oxidative stress and the state-of-the-art methods used to measure them. They will be able to seek and make use of appropriate published literature to examine associations of oxidative stress with age-related diseases.

Overall, this special field course will provide students with means to understand a multidisciplinary field of biomedical research, which focuses on documenting the involvement of free radicals in disease development and progression, its biological consequences and related therapeutic implications.


48


This course will review and capitalize on current findings from groundbreaking, interdisciplinary research in the field. Lectures:

• The basic chemistry of free radicals

• Sources of oxidative stress

• Antioxidant defenses

• Cellular, tissue responses to oxidative stress

• Techniques for the measurement of oxidative stress

• Biomarkers

• Free radicals in disease development and progression

• Evaluation of current preventive and therapeutic strategies

• Future challenges and research perspectives for Master students

Exercises: In an evidence-based, collaborative approach, students will be trained to incorporate concepts, methodology and applications into the analysis and evaluation of a pre-defined research project.

Assessment: Individual PowerPoint presentation on a specific topic in the field of free radical research. The module is graded.

Teaching style: Lectures. Interactive learning, problem-oriented exercises. Guest lecture. On-site visits.

Indicative Sources: Free Radicals in Biology and Medicine. Fourth Edition. Barry Halliwell and John Gutteridge. ISBN13: 9780198568698ISBN10


49

Module: Special Fields in Biology: Stem Cells


Course leader: Prof. Dr. Edda Tobiasch

Lecturer: Prof. Dr. Edda Tobiasch

Language: English


Special Field 3rd Semester MSc Biomedical Sciences



Tutorial: 2 credit hours



Lecture: 30 15


Total: 90 90


Credits: 6 ECTS


none

Recommendations: Knowledge in cell culture from previous study courses General Safety Instructions for working in laboratories

Safety Instructions for working with S1 organisms

Learning outcomes: Lecture: At the end of the course, students are able to work independently in scientific projects and are familiar with

1. the differences between embryonic and adult stem cells

2. the sources for stem cells

Tutorial: 3. ethical aspects of working with stem cells

4. current findings

Laboratory course: 4. markers for differentiation lines

5. detection methods of various differentiation lines Thus they will be able to work in laboratories of industries and universities, which focus on the


50

development of stem cell therapies with only a short training period for adjustments.


Lecture: The lecture focuses on the following questions and aspects: stem cells vs. progenitor cells, embryonic vs. adult stem cells: advantages and disadvantages, sources for stem cells, iPS, plasticity and potency, differentiation and transdifferentiation, isolation and purification, differentiation lines and markers, line-specific staining Tutorial: Each student has to present a paper which is related to his/her specific project and discuss the content with respect to their own current data and adjust the project, if applicable. Ethical aspects will be discussed. The data will be presented at an international or national conference if enough scientific results can be achieved. Laboratory course: Each student will have an own part of a scientific project to work on. This subproject will be part of a doctoral thesis or will partially overlap with the other subprojects. All components together will be a complete scientific project.

Assessment: The module is graded. The mode of assessment will be announced by the beginning of the module. Active participation and attendance is required to pass.

Teaching style: Lecture: PowerPoint, Overhead, black board Tutorial: paper, PowerPoint, black board


Turksen, Kursad: Adult stem cells, Humana Press

Sell, Stewart: Stem cells handbook, Humana Press

Chiu, Arlene Y.: Human embryonic stem cells, Humana Press

Artmann G.M., Hescheler J., Minger S.: Stem Cell Engineering, Springerverlag

Paolo Di Nardo: Adult Stem Cell Standardization, River Publishers Kasper, Cornelia; Witte, Frank; Pörtner, Ralf: Tissue Engineering III: Cell – Surface interactions for Tissue Culture, Springer-Verlag


51

Module: Special Fields in Biology: Physiology of the Skeletal System and Biomaterials

Semester: 3rd Semester in the MSc Biomedical Sciences

Course leader: Thomas Winkler & Arne Hothan

Lecturer: Thomas Winkler & Arne Hothan

Language: English

Assignment to curriculum: Special Field 3rd Semester MSc Biomedical Sciences



Tutorial: 2 credit hours Laboratory work: 2 credit hours



Lab work: 30 30


Credits: 6 ECTS


None


Learning outcomes: Lecture: After successfully completing the lecture the students are familiar with the anatomy and the biology of the human skeletal system. They know about the chemical composition, the cell biology as well as the physiology of the skeleton. Additionally they have learnt the musculoskeletal disorders and corresponding treatment options.

The students possess an overview of available treatment strategies. The method of tissue engineering to treat defects of the human joint is known. They are familiar with the application of implants as well as their specialties and the demands of the applied materials. The approval procedures on the German and the international market will extend their knowledge to a more market-based view.

Tutorial: The students are familiar with methods for the preparation of scientific presentations containing literature reviews, the preparation of a handout and


52

the talk itself. The presentation will be discussed and integrated into the acquired knowledge. Additionally the students will get an overview of the current research.

Laboratory course: The students can perform standardized tests following DIN standard specifications for in vitro testing of biomaterials.


Lecture:

• Physiology of the skeletal system

• Skeletal disorders and its treatment

• Tissue Engineering

• Biomaterials

• Implant types

• Approval of implants

Tutorial:

• Literature seminar to state-of-the-art publications

Laboratory course: Accomplishment of cytotoxic tests according to DIN EN ISO 10993-5 (biological assessment of medical products - part 5: Testing of in vitro cytotoxicity (ISO 10993-5:2009); German version EN ISO 109933-5:2009)

Assessment: Modul examination – graded Written examination Laboratory course: Active participation verified by individual protocols of experiments and measurements

Teaching style: Lecture: PowerPoint, Overhead, Board Tutorial: Written compilation of exercises, Overhead, Board


Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism, Clifford J. Rosen, Juliet E. Compston, Jane B. Lian

Bones and Cartilage: Developmental Skeletal Biology. Developmental Skeletal Biology: Developmental and Evolutionary Skeletal Biology,

Brian Keith Hall

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