Ministerio de Agricultura,Pesca y Alimentación - …...Cazorla (Sierra de Cazorla), Huelma (Sierra Mágina), Jaén (Campiña de Jaén), Linares (Sierra Morena/Campiña de Jaén),

REPORT ON THE FREEDOM DECLARATION OF

THE BLUETONGUE VIRUS SEROTYPE 1 AND 4

ON THE CENTRAL AND SOUTHERN AREA OF

SPAIN MAINLAND

January 2020

Subdirección General de Sanidad e Higiene Animal y Trazabilidad

Dirección General de Sanidad de la Producción Agraria

MAPA- ESPAÑA

MINISTERIO DE AGRICULTURA, PESCA Y ALIMENTACIÓN

DIRECCIÓN GENERAL DE SANIDAD DE LA PRODUCCIÓN AGRARIA

SUBDIRECCIÓN GENERAL DE SANIDAD E HIGIENE ANIMAL Y TRAZABILIDAD

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1) INTRODUCTION The purpose of this report is to provide the Commission with information demonstrating the absence of BTV-4 circulation in the regions of Azuaga, Castuera, Don Benito, Herrera del Duque, Mérida and Zafra in the province of Badajoz and in the regions of Cáceres, Logrosán (Zorita), Navalmoral de la Mata, Plasencia and Trujillo in the province of Cáceres (Autonomous Community of Extremadura); in the regions of Arenas de San Pedro, Candeleda, El Barco de Ávila and Sotillo de la Adrada in the province of Ávila (Autonomous Community of Castilla y León); in the provinces of Toledo and Ciudad Real; in the regions of Alcaraz, Balazore, Elche de la Sierra, Villarrobledo and Yeste in the province of Albacete (Autonomous Community of Castilla-La Mancha); in the regions of Alcalá la Real (Montes Occidentales), Beas de Segura (Sierra Segura), Cazorla (Sierra de Cazorla), Huelma (Sierra Mágina), Jaén (Campiña de Jaén), Linares (Sierra Morena/Campiña de Jaén), Santiesteban del Puerto (El Condado) and Úbeda (La Loma) in the province of Jaén; in the region of Aracena (Sierra Oriental) in the province of Huelva and in the region of Cazalla de la Sierra (Sierra Norte) in the province of Sevilla (Autonomous Community of Andalucía).

There is also information provided to demonstrate the absence of BTV-1 circulation in the province of Badajoz (Autonomous Community of Extremadura); and in the regions of Alcalá la Real (Montes Occidentales), Beas de Segura (Sierra Segura), Cazorla (Sierra de Cazorla), Huelma (Sierra Mágina), Jaén (Campiña de Jaén), Linares (Sierra Morena/Campiña de Jaén), Santiesteban del Puerto (El Condado) and Úbeda (La Loma) in the province of Jaén; in the region of Aracena (Sierra Oriental) in the province of Huelva and in the region of Cazalla de la Sierra (Sierra Norte) in the province of Sevilla (Autonomous Community of Andalucía).

The objective is to declare these territories as free of BTV-1 and 4 and in accordance with the requirement of absence of viral circulation for two vector activity seasons set out in point 2 of Article 6 of Regulation 1266/2007, of 26 October 2007, as well as surveillance requirements established in Annex I of the above mentioned regulation. 2) BLUETONGUE EPIDEMIOLOGICAL EVOLUTION IN SPAIN

The first virus incursion in Spain, since the fifties, was in 2000, when serotype 2 was notified in the Autonomous Community of Islas Baleares. As a consequence of the detection in 2000 of serotype 2, a national eradication and surveillance programme was implemented in Spain in order to early detect any further circulation of the BTV and to eradicate it by vaccination of ovine animals with attenuated vaccine. The afore-mentioned programme successfully led to the eradication of serotype 2 in 2002.

Since 2002 three BTV serotypes have been detected in Spain:

BTV-1: It has its origin in Northern Africa and was detected for the first time in Spain in July 2007. Currently, the south-western part of the Spanish mainland is restricted zone for this serotype.

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BTV-4: It was also sourced from Northern Africa and was first detected in November 2003 in Menorca island. At present the south-west of the Spanish mainland is restricted zone for this serotype.

BTV 8: It was sourced from Netherlands and it spread all over Europe. It was first detected in Spain in January 2008. Spain recovered its free status for this serotype in January 2013.

The following table summarizes the BTV outbreaks reported of serotypes 1 and 4 up to 2014.

Table 1: Comparison between the numbers of outbreaks of serotype 1 and 4 up to 2014.

BTV-1 AND 4 EVOLUTION SINCE 2015

In 2015, a restricted zone was established for serotype 4 and serotypes 1-4 in the south-west of the Spanish mainland, and the rest of the national territory was a bluetongue-free zone (see map 1).

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Map 1: Restricted zones in June 2015.

From 2015-2016 onwards, four consecutive vaccination campaigns were established in the restricted zones by means of Orden AAA/1424/2015, which led to a reduction in the total number of outbreaks in 2015-2016 of both serotypes (see map 2):

- 11 BTV-4 outbreaks in the provinces of Albacete, Cádiz, Jaén and Córdoba.

- 9 BTV-1 outbreaks in the provinces of Cádiz, Córdoba, Huelva and Sevilla.

Map 2: Bluetongue outbreaks during 2015-2016 season.

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In 2015-2016 season, due to the location of the outbreaks, it was necessary to extend the restricted zone for S4 to the whole province of Jaén, and new regions in the province of Albacete, where an emergency vaccination was also developed. In addition, regions in Extremadura that did not have compulsory vaccination against BTV-1 were included in the restricted zone (see map 3).

Map 3: Restricted zones since January 2016.

The high vaccination coverage drastically reduced the number of BTV-4 outbreaks compared to previous years (more than 97%), as well as the number of affected provinces that went from 9 to 4, 3 of them in Andalucía and 1 in the southeast part of Castilla-La Mancha (see graphic 2).

0

50

100

150

200

250

300

350

400

450

0

500000

1000000

1500000

2000000

2500000

2010 2011 2012 2013 2014 2015 2016*

Bovinos en zona de restricción Bovinos vacunados S4 Focos por temporada

Graphic 2: BTV-4 outbreaks /number of vaccinated animals.

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The effect of the vaccination campaign was maintained in the following season (2016-2017), where no BTV-4 outbreaks were reported in the country.

With regard to BTV-1, in 2016-2017 season, a total of 19 outbreaks were detected, all of them in restricted areas of Andalucía, Badajoz and Ciudad Real (see map 4).

Map 4: BTV outbreaks in 2016-2017 season.

In 2017-2018 season, there were 4 outbreaks of S4 in Cádiz and Huelva provinces and 6 outbreaks of S1 in Cádiz and Jaén provinces (see map 5), all in sentinel farms and therefore not vaccinated due to the surveillance programme in force.

Map 5: BTV outbreaks in 2017-2018 season.

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During the previous season (2018-2019), a total of 14 BTV-4 outbreaks have been reported in Cádiz, Córdoba, Málaga and Sevilla provinces (all in Andalucía) and no BTV-1 outbreaks have been reported (see map 6). All outbreaks were in sentinel farms and therefore in not vaccinated animals.

Map 6: BTV outbreaks in the current 2018-2019 season.

During the actual season (2019-2020), no outbreaks neither BTV-4 nor BTV-1 have been reported in Spain.

The following graphic represents an evolution summary of the outbreaks reported for both serotypes from 2014-2015 season until now:

2014 - 2015 2015 - 2016 2016 - 2017 2017 - 2018 2018 - 2019 2019 - 2020

Serotipo 1 13 9 19 6 0 0

Serotipo 4 397 11 0 6 14 0

0

50

100

150

200

250

300

350

400

450

Serotipo 1 Serotipo 4

Graphic 3: Comparision of outbreaks by serotype and season.

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We can therefore assert:

No BTV-4 outbreaks have been reported since 2014 in Azuaga, Castuera, Don Benito, Herrera del Duque, Mérida, neither in Zafra in the province of Badajoz (Extremadura).

BTV-4 outbreaks have never been reported in the region of Plasencia; and outbreaks have not been reported since 2014-2015 vectorial activity season in the regions of Cáceres, Logrosán (Zorita), Navalmoral de la Mata and Trujillo, all of them belonging to the province of Cáceres (Extremadura).

BTV-4 outbreaks have never been reported in the regions of Arenas de San Pedro, Candeleda, El Barco de Ávila and Sotillo de la Adrada in the province of Ávila (Castilla y León).

In the province of Toledo (Castilla-La Mancha) BTV-4 outbreaks have been reported in just one region (Belvís de la Jara) and the last notification was in 2014. We have the same situation in the province of Ciudad Real (Castilla-La Mancha), where BTV-4 outbreaks were just reported in 3 regions (Almadén, Almodóvar del Campo and Piedrabuena) and the last notification in all those regions was also in 2014. BTV-4 outbreaks have never been reported in the regions of Balazote, Elche de la Sierra, Villarrobledo and Yeste; and the last outbreak notified in the region of Alcaraz, all of them in the province of Albacete (Castilla-La Mancha), was in 2015.

BTV-4 outbreaks have never been reported in five of the nine regions of the province of Jaén (Andalucía), and the last outbreaks reported were in 2015 in the regions of Linares (Sierra Morena/Campiña de Jaén) and Beas de Segura (Sierra Segura), and in 2014 in the region of Úbeda (La Loma). Futhermore, BTV-4 outbreak have never been reported in the region of Aracena (Sierra Oriental) in Huelva, and the last notification in Cazalla de la Sierra (Sierra Norte) in Sevilla was in 2014.

BTV-1 outbreaks have never been reported in the region in Zafra, and no outbreaks have been reported since 2009 in the region of Badajoz and since 2016 in the regions of Azuaga, Castuera, Don Benito, Herrera del Duque, Jerez de los Caballeros and Mérida, all located in Badajoz (Extremadura).

BTV-1 outbreaks have never been reported in six of the nine regions of the province of Jaén (Andalucía), and the last outbreak reported in Santiesteban del Puerto (El Condado) was in 2010, and in Linares (Sierra Morena/Campiña de Jaén) was in 2017. The last outbreak reported in Aracena (Sierra Oriental) in Huelva was in 2008 and in 2016 in Cazalla de la Sierra (Sierra Norte) in Sevilla.

Therefore in all cases, we have more than two years without circulation, including two seasons of vectorial activity.

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3) RESTRICTED ZONES 3.1 Restricted zones included in Orden APA/385/2019: So far, Spain is divided into two zones (see map 7):

1) BTV-4 restricted zone:

a) In the Autonomous Community of Andalucía:

1.º Cádiz, Córdoba, Huelva, Jaén, Málaga and Sevilla provinces.

2.º In Granada province: Motril (Costa de Granada) region.

b) In the Autonomous Community of Castilla-La Mancha:

1.º Ciudad Real and Toledo provinces.

2.º In Albacete province: Alcaraz, Balazote, Elche de la Sierra, Villarrobledo and Yeste regions.

c) In the Autonomous Community of Castilla y León:

1.º In Ávila province: Arenas de San Pedro, Candeleda, El Barco de Ávila, and Sotillo de la Adrada regions.

d) The Autonomous Community of Extremadura.



1.º Cádiz, Córdoba, Huelva, Jaén, Málaga and Sevilla provinces.



1.º In Ciudad Real province: Almadén, Almodóvar del Campo and Piedrabuena (former regions of Piedrabuena y Horcajo de los Montes) regions.

c) In the Autonomous Community of Extremadura:

1º. Badajoz province.

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Map 7: Restricted zones. March 2019.

3.2 Proposal of new restricted zones

Considering the dates of the last BTV-1 and BTV-4 outbreaks that affected the zones proposed as free, the new restriction zones would be as follows (see map 8):



1.º Cádiz, Córdoba and Málaga provinces.


3º. In Huelva province: Almonte (Entorno de Doñana), Cartaya (Costa Occidental), Cortegana (Sierra Occidental), La Palma del Condado (Condado de Huelva), Puebla de Guzmán (Andévalo Occidental) and Valverde del Camino (Andévalo Oriental) regions.

4º. In Jaén province: Andújar (Sierra Morena/Campiña de Jaén) region.

5º. In Sevilla province: Cantillana (Vega de Sevilla), Carmona (Los Arcores), Écija (La Campiña), Lebrija (Las Marismas), Marchena (Serranía Sudoeste), Osuna (Campiña/Sierra Sur), Sanlúcar la Mayor (Poniente de Sevilla), Sevilla (Delegación Provincial) and Utrera (Bajo Guadalquivir) regions.

b) In the Autonomous Community of Extremadura:

1º. In Badajoz province: Badajoz and Jerez de los Caballeros regions.

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2º. In Cáceres province: Coria and Valencia de Alcántara region.



1.º Cádiz, Córdoba and Málaga provinces.

2º. In Granada province: Motril (Costa de Granada) region.

3º. In Huelva province: Almonte (Entorno de Doñana), Cartaya (Costa Occidental), Cortegana (Sierra Occidental), La Palma del Condado (Condado de Huelva), Puebla de Guzmán (Andévalo Occidental) and Valverde del Camino (Andévalo Oriental) regions.

4º. In Jaén province: Andújar (Sierra Morena/Campiña Jaén) region.

5º. In Sevilla province: Cantillana (Vega de Sevilla), Carmona (Los Arcores), Écija (La Campiña), Lebrija (Las Marismas), Marchena (Serranía Sudoeste), Osuna (Campiña/Sierra Sur), Sanlúcar la Mayor (Poniente de Sevilla), Sevilla (Delegación Provincial) and Utrera (Bajo Guadalquivir) regions.


1.º In Ciudad Real province: Almadén, Almodóvar del Campo and Piedrabuena regions.

Map 8: New restricted zones proposal. 2020.

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4) BLUETONGE SURVEILLANCE, CONTROL AND ERRADICATION NATIONAL PROGRAM IN SPAIN.

4.1) Legal basis:

At European Union level

o Council Directive 2000/75/EC, of 20 November, laying down specific provisions for the control and eradication of bluetongue.

o Commission Regulation (EC) No 1266/2007, of 26 October, on implementing rules for Council Directive 2000/75/EC as regards the control, monitoring, surveillance and restrictions on movements of certain animals of susceptible species in relation to bluetongue.

At national level:

o Royal Decree 1228/2001, of 8 November, laying down specific provisions for the control and eradication of bluetongue.

o Royal Decree 617/2007, of 16 May, laying down the list of animal diseases whose notification is compulsory and lists the rules for its notification, includes Bluetongue as a disease whose notification is compulsory, not only to the OIE but also to the European Union.

o After the reoccurrence of the disease in 2004 specific provisions for the protection against bluetongue were laid down in subsequent ministerial orders that were focused on the vaccination programme and control of movements. These legal acts published in subsequent years allowed the competent authorities to adapt the control measures to the epidemiological evolution of the disease. The latest of these is Order AAA/1424/2015 and its subsequent amendments.

4.2) Program’s description:

With the objectives of early detection of new serotypes of BTV and to eradicate them, a national programme was enforced in 2000, covering the whole national territory.

This programme is based on a comprehensive legal basis at European Union level and at national level, making the provisions of the programme compulsory for the stakeholders and, thus, ensuring a high level of implementation of the disease control policy.

This programme is based on the following:

1. Surveillance programme which includes 3 types of surveillance:

a. Serological and virological surveillance in sentinel animals

b. Passive clinical surveillance

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c. Entomological surveillance

2. Vaccination programme

3. Control of movements

The measures were tailored to the epidemiological situation of each part of Spain.

4.2.1) Surveillance programme

Serological and virological surveillance in sentinel animals

This surveillance programme based on sentinel animals represents a highly effective early detection programme covering the whole territory of Spain, and it is aimed to an early detection viral circulation, not only for serotypes existing in the country but also for new serotypes that might appear.

It is based on periodical testing of sentinel animals. Those tests are reinforced in risk areas (highlighted in red in map 9).

Map 9: Areas at risk in Spain for surveillance in 2019.

Sentinels animals could be cattle, sheep or goats (preferably cattle). Animals should be over 4 months old, not vaccinated and previously seronegative. It is also compulsory that the animals had been at least for the last 30 days in the province subject to the study.

The minimum number of sentinel animals per geographical unit of reference (province level) is able to detect a prevalence of 5% with 95% confidence in each geographical unit of reference. That implies to test 59 sentinel animals in every province. These 59 animals per province should be located in, at least, 6 different holdings of this province in order to ensure a representative sampling.

The testing frequency varies considering whether the animals are farmed in a high risk area or not:

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High risk areas:

- Balearic islands and Almería, Cádiz, Córdoba, Granada, Huelva, Jaén, Málaga, Sevilla, Badajoz, Cáceres, Albacete, Ciudad Real, Toledo, Salamanca, Barcelona, Gerona, Lérida, Huesca, Zaragoza, Navarra, Álava, Guipúzcoa y Vizcaya provinces.

9 samples per year (monthly from May to January).

It means a total minimum number of 13.275 samples (see detailed data in Annex II).

Out of the high risk areas:

- Rest of Spanish mainland and Canary Islands.

2 samples per year (in May and in December).

It means a minimum total number of 2.714 samples (see detailed data in Annex II).

The diagnostic techniques for the Serological and Virological Surveillance Program are as follows:

Serological techniques

o Competitive ELISA: available on each diagnose laboratory for Bluetongue.

o Serum neutralization: technique which enables the serological confirmation and it is available against every serotype of the virus in National Bluetongue Reference Laboratory (LCV Algete).

Virological techniques

o Detection of the genome of the virus: this involves the real-time RT-PCR technique specific to the serogroup, recommended by the EU RL and transferred by the NRL during the meeting of Animal Health Laboratories held in Madrid in April 2014, using the primers/probes described in Hofmann et al., 2008.

o Identification of the virus serotype using the RT-PCR technique specific to the serotype and sequencing.

The routine testing implies taking samples which are analyzed to detect antibodies by an ELISA test at the Autonomous Region Laboratories. If any animal tested show a positive result, blood samples are analyzed by generic RT-PCR in the regional laboratory (if this technique is available in the regional laboratory). In case of positive results, the samples are sent to National Reference Laboratory in order to confirm or to rule out current viral circulation by virological techniques or seroneutralization.

Passive clinical surveillance

This surveillance is very useful as a reinforcement of the serological-virological surveillance and for detection of circulation of new virus serotypes in the zone. In addition, it is very effective due to the enormous awareness, both in the sector and

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among veterinary practicioners, in the detection of symptoms of the disease and its subsequent compulsory notification to the competent authorities on the basis of animal health legislation.

Following the communication of a suspicion further investigation should be carried out by the competent authority in order to confirm or to rule out the presence of BTV. The investigation’s outcome should be property consigned on a document based system.

Furthermore, the bluetongue contingency manual establishes guidelines in cases of suspicion which conforms an early warning system.

Entomological surveillance

The entomological surveillance program has been implemented in Spain since 2004, so Spain has been able to gather a high amount of data about the species, geographical location, densities and activity period of bluetongue transmission-responsible vectors (Culicoides imicola, Culicoides obsoletus complex and other Culicoides spp.). This data have been used to create predictive models of absence / presence of these vectors in different climatological conditions and have shown a highest vector presence from April to October with a density peak for C. obsoletus in May-July and for C. imicola in September-October (see maps 10 and 11):

Maps 10-11: Predictive maps of presence/absence of C. imicola and C. obsoletus in the Iberian Peninsula

Over the years, the number of permanent traps has changed, starting in 2009 with 95 permanent traps (see map 12), to which was added the use of 150 reinforcement traps between 2010 and April 2011.

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Map 12: Permanent traps 2009.

Since May 2011, 75 permanent traps equipped with ultraviolet light have been used, some working throughout the year and others during the free period of the vector. In 2013, the number of permanent traps was reduced to a total of 28, a number that is updated according to the needs and since 2018 program, it represents a set of 30 traps, distributed considering 4 zones (see map 13):

- The North zone that corresponds to the main area of distribution and abundance of Culicoides obsoletus: 13 sampling points.

- The southern area corresponding to the main area of distribution and abundance of Culicoides imicola: 7 sampling points.

- The central zone, which corresponds to the upper part of the distribution of Culicoides imicola and coincides with the southern part of the zone where Culicoides obsoletus is most abundant: 6 sampling points.

- Islas Canarias y Baleares: 2 sampling points in Canary Islands and 2 in Baleares.

Map 13: Permanent traps since 2018.

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4.2.2) Vaccination program

The bluetongue vaccination programme is currently specified in Orden APA/385/2019 of 14 July amending Orden 1424/2015 of 14 July laying down specific protection measures relating to bluetongue.

This legislation makes compulsory to vaccinate ovine and bovine animals over 3 months of age against BTV-1 and 4 in their respective restricted zones.

As a summary of the vaccination program it is important to highlight:

- On restricted areas:

a) A compulsory vaccination program against BTV-4 has been implemented in the last years in Extremadura, center and west Andalucía mainland, some regions of Albacete and the provinces of Ciudad Real and Toledo in Castilla-La Mancha and the southern regions of the province of Ávila in Castilla y León.

b) A compulsory vaccination program against BTV-1 has been implemented in the last years in the province of Badajoz in Extremadura, center and west Andalucía and the western regions of the province of Ciudad Real province in Castilla-La Mancha.

c) The vaccination described in paragraphs (a) and (b) will be carried out for at least one additional campaign in the regions remaining in the restricted zone with the aim of achieving the final eradication of the disease.

- On the zone intended to be free from:

a) BTV-4 has been implemented a compulsory vaccination against this serotype in the previous years.

b) BTV-1 has been implemented a compulsory vaccination against this serotype in the previous years.

4.2.3) Control of movements

Zoning and control of movements are key issues of the BT control policy when viral circulation is present.

The rules for intra-Community movement are defined according to Commission Regulation (EC) No 1266/2007 of 26 October 2007 and subsequent amendments laying down detailed rules for the implementation of Council Directive 2000/75/EC as regards the control, monitoring, surveillance and restrictions on movements of certain animals of species susceptible to bluetongue.

The national movement rules have been regulated according to the epidemiological situation in different legislation acts, the latest of which is Orden AAA/1424/2015 of 14 July laying down specific requirements in relation to bluetongue, as lastly amended by Orden APA/385/2019 of 14 July.

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5) OUTCOME OF BTV-1 VIRUS SURVEILLANCE, CONTROL AND ERADICATION PROGRAM 5.1) Outcome of the Serological and Virological Surveillance Program:

A summary of the layout of the Bluetongue Surveillance Program on years 2016, 2017 2018 and 2019 can be found endorsed as Annex III.

In 2019 and up to date, all laboratory tests carried out within the framework of the surveillance programme have been rendered negative, demonstrating that there has not been viral circulation in the areas proposed as free.

All samples analysed since 2014 in the region of Belvís de la Jara (Toledo), Almaden, Almodovar del Campo and Piedrabuena (Ciudad Real), Cáceres, Navalmoral de la Mata and Trujillo (Cáceres) and Úbeda (Jaén), and 2015 in Alcaraz (Albacete), Logrosán (Cáceres), Linares and Beas de Segura (Jaén) and Cazalla de la Sierra (Sevilla), that are proposed to be declared free rendered a negative result for BTV-4.

All samples analysed since 2008 in Aracena (Huelva), 2010 in Santisteban del Puerto (Jaén), 2016 in the province of Badajoz and Cazalla de la Sierra (Sevilla), and 2017 in Linares (Jaén), that are proposed to be declared free rendered a negative result for BTV-1.

All samples analysed in the rest of regions proposed to be declared free, historically rendered a negative result.

5.2) Vaccination data

Detailed data on vaccination program for recent years are available in Annex I.

6) BTV-1 AND 4 EPIDEMIOLOGICAL SITUATION IN NEARBY COUNTRIES

Considering its geographical situation, the countries that might affect the situation in Spain are mainly France, Portugal and Northern African Countries.

According to the OIE and ADNS information:

Regarding Portugal, no BTV outbreaks have been reported during 2019. The last BTV-4 outbreak was notified in November 2018, and the last BTV-1 outbreak declared was in January 2017. Portugal is currently a restricted zone for both serotypes throughout its continental territory.

In reference to France, no case of BTV-1 has been declared in France mainland since September 2013. For serotype 4, the whole country is restricted zone for BTV-4 and the last outbreak reported in the continental area of France was in September 2018.

In Morocco, viral circulation of BTV-1 and 4 was present during 2019 in the center and south of the country.

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It is very important to coordinate the control policy with nearby countries, so Spain keeps a close relationship in Animal Health especially with Portugal, France and Morocco, and rest of Mediterranean countries via REMESA (Mediterranean Animal Health Network).

Once a year bilateral meetings are held Spain-France and Spain-Portugal for general health animal purposes and frequently specific meetings about Bluetongue. There are important in order to share information and experiences, to adopt measures related to animal movements, to coordinate the common control strategy of the disease and to share material and technical resources (vaccines, diagnostic methods, etc.).

7) CONCLUSIONS

1. No BTV-4 outbreaks have been declared since 2014 in Belvís de la Jara (Toledo), Almadén, Almodóvar del Campo and Piedrabuena (Ciudad Real), Cáceres, Navalmoral de la Mata and Trujillo (Cáceres), Úbeda (Jaén), Cazalla de la Sierra (Sevilla), and 2015 in Alcaraz (Albacete), Logrosán (Cáceres), Linares and Beas de Segura (Jaén), and no outbreaks have been declared in the rest regions intended to be free.

2. No BTV-1 outbreaks have been declared since 2008 in Aracena (Huelva), 2010 in Santisteban del Puerto (Jaén), 2016 in the province of Badajoz and Cazalla de la Sierra (Sevilla), and 2017 in Linares (Jaén), and no outbreaks have been declared in the rest regions intended to be free.

3. The national surveillance and control program, has been proved to be able to detect any viral circulation of BT.

4. Any movement of animals from all bordering countries, is controlled on the basis of the requirements laid down in Regulation 1266/2007.

5. The number of outbreaks has been reduced in recent years in the southern regions of Castilla y León, in Castilla-La Mancha, Extremadura, and Jaén (Andalucía) and the surrounding areas, reaching the current epidemiological silence.

6. There is a compulsory vaccination campaign against both serotypes planned for 2020 in the areas that would remain as restricted zones.

7. Orden AAA/1424/2015 sets out guidelines for the control of national movements of bluetongue-susceptible animals from restricted areas, providing adequate health guarantees to prevent the spread of the disease.

8. An appropriated coordination with nearby countries has been achieved, and in particular with the rest of EU members and Northern African countries, in order to maintain a continuous exchange of epidemiological information among the countries in the area, and develop common control strategies.

9. The monitoring and surveillance program implemented in Spain includes the requirements set out in points 1.3 and 1.1.2.1 of Annex I of Regulation 1266/2007, of 26 October 2007, as regards the monitoring to provide the Commission with substantiated information proving the absence of bluetongue viral circulation in an epidemiological relevant geographical area during a period of two years.

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10. According to BT monitoring and surveillance program results, there has not been BTV-4 and BTV-1 circulation in the territories to be declared as free (including the requirement of two full periods of vector activity). Therefore, all territories proposed to be free, comply with the conditions set out in Annex I of Regulation 1266/2007.

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ANNEX I

VACCINATION PROGRAMME. SPAIN 1.- BOVINE 2016

Autonomous Community

Total number of herds in

the programme

Total number of animals in

the programme

Vaccination programme data:

BTV-4 vaccinated

herds

BTV-4 vaccinated

animals

BTV-4 doses

administred

BTV-1 vaccinated

herds

BTV-1 vaccinated

animals

BTV-1 doses administred

ANDALUCÍA 9.692 521.907 6.170 527.307 743.130 6.187 527.096 747.740

ARAGÓN 3.072 332.730

ASTURIAS 18.161 384.932

BALEARES 566 29.834

CANARIAS 896 19.481

CANTABRIA 7.125 269.974

CASTILLA-LA MANCHA 3.149 412.223 2.718 414.329 676.915 1.502 173.187 244.264

CASTILLA Y LEÓN 17.495 1.380.302 1732 118.329 175.659 441 31.782 58.145

CATALUÑA 4.996 611.676

EXTREMADURA 10.907 944.342 9.817 897.369 1.302.852 9.815 898.029 1.467.173

GALICIA 42.177 954.529

LA RIOJA 319 40.196

MADRID 1.410 83.097 1055 74.550 106.921

MURCIA 339 76.845

NAVARRA 1.569 115.880

PAÍS VASCO 5.472 135.737

VALENCIA 621 54.532

TOTALES 127.966 6.368.217 21.492 2.031.884 3.005.477 17.945 1.630.094 2.517.322

2017



the programme


the programme


BTV-4 vaccinated

herds

BTV-4 vaccinated

animals

BTV-4 doses

administred

BTV-1 vaccinated

herds

BTV-1 vaccinated

animals


ANDALUCÍA 6.734 569.545 6.124 543.830 775.587 6.128 543.770 777.277

ARAGÓN 3.103 336.408

ASTURIAS 16.503 386.326

BALEARES 550 29.264

CANARIAS 796 16.858

CANTABRIA 7.046 285.925

22


CASTILLA Y LEÓN 17.553 1.088.214 246 15.616 19.858 1561 104.985 147.436

CATALUÑA 4.943 633.535

EXTREMADURA 11.073 968.602 9.958 943.922 1.352.426 9.960 944.455 1.356.972

GALICIA 40.688 938.615

LA RIOJA 319 43.303

MADRID 1.398 84.459 1048 75.339 110.920

MURCIA 354 79.867

NAVARRA 1.553 123.496

PAÍS VASCO 5.474 131.896

VALENCIA 625 55.489

TOTALES 121.836 6.214.797 20.106 2.031.281 2.985.015 19.153 1.774.487 2.536.631

2018



the programme


the programme


BTV-4 vaccinated

herds

BTV-4 vaccinated

animals

BTV-4 doses

administred

BTV-1 vaccinated

herds

BTV-1 vaccinated

animals


ANDALUCÍA 6.673 578.045 6.143 552.631 779.728 6.141 552.764 780.859

ARAGÓN 3.189 346.403

ASTURIAS 15.733 389.946

BALEARES 553 28.925

CANARIAS 746 19.072

CANTABRIA 6.940 284.429


CASTILLA Y LEÓN 17.106 1.412.357 1.570 105.886 138.241 260 17.239 21.493

CATALUÑA 4.960 650.832

EXTREMADURA 1.143 975.404 10.046 958.099 1.392.971 10.048 958.467 1.395.137

GALICIA 39.627 930.663

LA RIOJA 322 44.457

MADRID 1.434 87.714 1.019 68.960 103.267

MURCIA 362 66.743

NAVARRA 1.541 122.372

PAÍS VASCO 5.320 134.703

VALENCIA 609 54.672

TOTALES 109.615 6.617.128 21.482 2.167.552 3.180.791 17.935 1.714.651 2.459.275

23

2019*



the programme


the programme


BTV-4 vaccinated

herds

BTV-4 vaccinated

animals

BTV-4 doses

administred

BTV-1 vaccinated

herds

BTV-1 vaccinated

animals


ANDALUCÍA 6593 577.856 4.986 387.847 483.373 4.987 381.895 484.352

ARAGÓN 3189 347.089

ASTURIAS 16639 385.703

BALEARES 553 28.925

CANARIAS 746 19.072

CANTABRIA 6940 284.429

CASTILLA-LA MANCHA 3357 490.391 2.380 351.601 524.880 589 71.290 91.598

CASTILLA Y LEÓN 16719 1.500.413 849 45.235 55.789 30 949 1.165

CATALUÑA 4960 650.832

EXTREMADURA 11073 968.602 9.230 776.478 1.002.211 3.513 310.018 401.528

GALICIA 39645 719.112

LA RIOJA 312 43.480

MADRID 1434 87.614

MURCIA 360 67.129

NAVARRA 1541 122.372

PAÍS VASCO 5109 139.323

VALENCIA 601 54.857

TOTALES 119.771 6.487.199 17.445 1.561.161 2.066.253 9.119 764.152 978.643

* 2019 data correspond to the first semester of the year.

2.- OVINE 2016



the programme


the programme


BTV-4 vaccinated

herds

BTV-4 vaccinated

animals


BTV-1 vaccinated

herds

BTV-1 vaccinated

animals


ANDALUCÍA 24.722 3.430.450 8.397 1.505.284 1.964.827 8.419 1.505.407 1.969.556

ARAGÓN 3.592 1.387.839

ASTURIAS 8.278 95.347

BALEARES 4.293 322.384

CANARIAS 1.941 249.366

CANTABRIA 9.592 88.042

CASTILLA-LA MANCHA 6.603 2.933.031 3.515 1.351.203 1.632.917 1.979 589.829 678.917

CASTILLA Y LEÓN 9.162 3.052.545 522 49.895 66.486 125 16.434 25.232

CATALUÑA 3.574 472.023

24

EXTREMADURA 17.714 3.696.780 9.726 2.770.579 3.286.821 9.742 2.779.073 3.866.053

GALICIA 22.414 212.213

LA RIOJA 411 105.739

MADRID 678 94.735 521 69.819 92.016

MURCIA 1.903 893.047

NAVARRA 2.462 513.972

PAÍS VASCO 8.467 264.577

VALENCIA 1.414 393.054

TOTALES 127.220 18.205.144 22.681 5.746.780 7.043.067 20.265 4.890.743 6.539.758

2017



the programme


the programme


BTV-4 vaccinated

herds

BTV-4 vaccinated

animals


BTV-1 vaccinated

herds

BTV-1 vaccinated

animals


ANDALUCÍA 18.370 3.581.048 8.569 1.558.775 2.074.845 8.595 1.556.411 2.080.204

ARAGÓN 3.567 1.381.686

ASTURIAS 8.413 94.538

BALEARES 4.171 280.000

CANARIAS 2.405 254.036



CASTILLA Y LEÓN 9.761 3.016.276 474 45.067 55.113 114 15.856 17.786

CATALUÑA 3.647 464.095

EXTREMADURA 17.013 4.008.221 9.713 2.789.511 3.229.698 9.739 2.794.656 3.249.052

GALICIA 22.623 200.059

LA RIOJA 405 99.023

MADRID 593 72.002 490 62.822 82.133

MURCIA 1.881 881.499

NAVARRA 2.777 509.177

PAÍS VASCO 8.988 264.953

VALENCIA 1.425 394.590

TOTALES 118.196 18.681.287 22.800 5.834.354 7.053.286 20.403 4.955.228 6.016.486

25

2018



the programme


the programme


BTV-4 vaccinated

herds

BTV-4 vaccinated

animals


BTV-1 vaccinated

herds

BTV-1 vaccinated

animals


ANDALUCÍA 18.088 3.628.356 8.565 1.551.464 1.789.962 8.606 1.553.968 2.006.927

ARAGÓN 3.487 1.375.050

ASTURIAS 8.232 76.774

BALEARES 4.120 224.096

CANARIAS 3.089 271.226



CASTILLA Y LEÓN 9.521 2.806.016 464 46.917 47.449 99 13.888 15.205

CATALUÑA 3.615 435.155

EXTREMADURA 17.067 4.021.018 9.495 2.742.828 3.079.823 9.532 2.753.911 3.208.324

GALICIA 21.747 255.772

LA RIOJA 396 98.538

MADRID 560 65.465 503 62.270 82.133

MURCIA 1.841 867.092

NAVARRA 2.505 484.698

PAÍS VASCO 6.289 253.656

VALENCIA 1.327 368.866

TOTALES 112.488 18.169.535 22.451 5.796.381 6.420.752 20.149 4.912.203 5.896.333

2019*



the programme


the programme


BTV-4 vaccinated

herds

BTV-4 vaccinated

animals


BTV-1 vaccinated

herds

BTV-1 vaccinated

animals


ANDALUCÍA 17.653 3.592.810 4.342 797.954 878.632 4.462 804.787 942.095

ARAGÓN 3.567 1.410.746

ASTURIAS 9.024 113.160

BALEARES 4.120 224.096

CANARIAS 2.283 254.154


CASTILLA-LA MANCHA 6.480 2.895.985 3.497 1.106.287 1.110.621 985 400.311 403.306

CASTILLA Y LEÓN 10.160 2.852.298 258 20.071 20.371

CATALUÑA 3.615 435.155

EXTREMADURA 16.089 4.008.221 6.800 2.284.537 2.382.799 4.405 1.645.938 1.715.494

GALICIA 21.769 186.381

LA RIOJA 382 94.000

MADRID 668 90.893

26

MURCIA 1.841 867.092

NAVARRA 2.505 484.698

PAÍS VASCO 8.158 243.984

VALENCIA 1.380 371.833

TOTALES 116.020 18.220.652 14.897 4.208.849 4.392.423 9.852 2.851.036 3.060.895

* 2019 data correspond to the first semester of the year.

27

ANNEX II

MINIMUM SAMPLING ACCORDING TO THE NATIONAL

SURVEILLANCE PROGRAMME

I. MINIMUM SAMPLING OF SENTINEL ANIMALS IN HIGH RISK AREAS.

AUTONOMOUS COMMUNITY

PROVINCE ANIMALS

TO BE SAMPLED

SAMPLING/YEAR

TOTAL NUMBER

OF SAMPLES

ANDALUCÍA

Almería 59 9 472

Cádiz 59 9 472

Córdoba 59 9 472

Huelva 59 9 472

Jaén 59 9 472

Granada 59 9 472

Málaga 59 9 472

Sevilla 59 9 472

CASTILLA-LA MANCHA

Toledo 59 9 472

Ciudad Real 59 9 472

Albacete 59 9 472

CASTILLA Y LEÓN

Ávila 59 9 472

Salamanca 59 9 472

EXTREMADURA Cáceres 59 9 472

Badajoz 59 9 472

MADRID Madrid 59 9 472

MURCIA Murcia 59 9 472

NAVARRA Navarra 59 9 472

PAÍS VASCO Toda la CA 59 9 472

ARAGÓN Huesca 59 9 472

Zaragoza 59 9 472

CATALUÑA

Barcelona 59 9 472

Lérida 59 9 472

Gerona 59 9 472

BALEARES Baleares 59 9 472

TOTAL

1.475

13.275

28

II. MINIMUM SAMPLING OF SENTINEL ANIMALS OUT OF HIGH RISK AREAS

AUTONOMOUS COMMUNITY

PROVINCE ANIMALS

TO BE SAMPLED

SAMPLING/YEAR TOTAL

NUMBER OF SAMPLES

ARAGÓN Teruel 59 2 118

ASTURIAS Asturias 59 2 118

CANTABRIA Cantabria 59 2 118

CASTILLA-LA MANCHA

Guadalajara 59 2 118

Cuenca 59 2 118

Burgos 59 2 118

León 59 2 118

Palencia 59 2 118

Segovia 59 2 118

Soria 59 2 118

Valladolid 59 2 118

Zamora 59 2 118

CATALUÑA Tarragona 59 2 118

GALICIA

A Coruña 59 2 118

Lugo 59 2 118

Orense 59 2 118

Pontevedra 59 2 118

LA RIOJA La Rioja 59 2 118

VALENCIA

Alicante 59 2 118

Castellón 59 2 118

Valencia 59 2 118

CANARIAS Las Palmas 59 2 118

SC de Tenerife 59 2 118

TOTAL

1.357

2.714

29

ANNEX III

Results of the serological and virological surveillance programme in the provinces where part of their territory is proposed to be free zone

1-2016

AUTONOMOUS COMMUNITY Province

Number of laboratory testing

Number of

animals sampeld ELISA PCR

Castilla-La Mancha

Toledo 666 9 106

Albacete 697 11 99


Castilla y León Ávila 500 5 147

Extremadura Cáceres 845 835 132

Badajoz 1059 1051 212

Andalucía

Huelva 736 735 350

Jaén 668 639 208

Sevilla 695 804 422

2-2017



Number of


Castilla-La Mancha

Toledo 758 16 113

Albacete 599 10 78




Badajoz 866 856 178

Andalucía

Huelva 614 613 208

Jaén 644 643 228

Sevilla 630 630 219

30

3-2018



Number of


Castilla-La Mancha

Toledo 747 37 112

Albacete 622 7 84




Badajoz 823 823 149

Andalucía

Huelva 528 527 140

Jaén 533 533 117

Sevilla 630 808 308

4-2019



Number of


Castilla-La Mancha

Toledo 735 8 119

Albacete 623 7 95




Badajoz 950 950 225

Andalucía

Huelva 568 517 171

Jaén 431 431 75

Sevilla 593 582 269

Documents

Ministerio de Agricultura,Pesca y Alimentación - …...Cazorla (Sierra de Cazorla), Huelma (Sierra Mágina), Jaén (Campiña de Jaén), Linares (Sierra Morena/Campiña de Jaén),