Micromanipulator System NT-88-V3 Series...The NT-88-V3 series, jointly developed by Nikon and Narishige, ensures easy operation and an unsurpassed level of precision. When used in

Micromanipulator System NT-88-V3 Series

ICSI (Intracytoplasmic sperm injection) and IMSI (Intracytoplasmic morphologicallyselected sperm injection), IVF (In vitro fertilization) technologies that have receiveda lot of attention in recent years as a remedy for male infertility, require a highlyprecise manipulator to respond to delicate movement of the operator's hands.

Stable, smooth manipulation is also required for injection to the stem cell,transgenics and electrophysiology.

The NT-88-V3 series, jointly developed by Nikon and Narishige, ensures easyoperation and an unsurpassed level of precision. When used in combination withNikon's high-performance microscopes, it provides strong support for cutting-edgebiomedical research.

•Size is greatly reduced as the 3D motor-driven coarse control manipulator and 3D hydraulic fine micromanipulator are combined in a single unit.

•Assembly of the micromanipulator is fast and easy due to the extremely stable, one-piece mounting adapter.

•Wide varieties of microinjectors for holding or injecting specimens depending on use.

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Oil-hydraulic Micromanipulators

3D Hydraulic Micromanipulator

NT-88-V3Combined 3D motor-driven coarse control manipulator andthe 3D hydraulic fine micromanipulator realize remarkablycompact and stable design.

• Less than half the size of a conventional model—minimal lengthtubes and cords—the manipulator fits easily into the incubator.

• Closer-to-the-center settings are possible, while reduceddistance from manipulator mount to microelectrode tipincreases stability.

• X-axis control knobs are located symmetrically on opposite sidesof the joystick to eliminate left side/right side positioningproblems.

• 10mm X-Y operating range provides versatility in manipulationat lower magnifications.

• A “resume” feature returns the pipette to the original positionafter the pipette has been raised for quicker, easier Petri dishchanges.

• The universal joint is outfitted with an angle gauge, whichensures accurate-angle operation. The forward-mounted returnmechanism facilitates Petri dish changing.

• Permits one-touch installation on an inverted microscope.

3D Hydraulic Micromanipulator (Single-hand type)

NT-88-V3SHSuitable for experiments that do not require the holding side’spipette, such as the microinjection or microdissection to anadherent cell

3D Hydraulic Micromanipulator (Manual Single-hand type)

NT-88-V3MSHA manual 3D coarse positioning manipulator allows you to quicklymove the micro-pipette to the ideal position. And the compactdesign allows you to position it closer to you with the controlknob in easy reach.

NT-88-V3 NT-88-V3SH NT-88-V3MSH

Hydraulic fine micromanipulator

Working range Control knob operation Max. 10mm

Joystick operation Max. 2mm

Displacement by Full turn of knob 250µmcontrol knob rotation Minimum graduation 2µm

Coarse manipulator

Working range Max. 22mm (motorized) Max. 30mm (manual)

Hanging joystick design for optimalergonomics and user comfort during longperiods of use

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Universal Joint

UJ-V3Universal Joint UJ-V3 allows the pipette holder to be moved in asingle direction. The angle can be adjusted for both vertical andhorizontal directions via two separate control knobs. The forward-mounted return mechanism facilitates changing the petri dishwhile the built-in angle gauge ensures accurate-angle operation.Moreover, because the UJ-V3 is mountable from above, unlike theconventional type, attachment and removal is much easier.

Microscope Adapters for NT-88-V3Various adapters are available for use with Nikon microscopes.As both adapters and manipulators are compact, attachingthem is very easy.

NS-TEV3Mounting Adapter to use withinverted microscopes Ti and TE2000

NS-T1Mounting Adapter to use withinverted microscope TS100

Pneumatic Microinjector IM-11-2* The IM-11-2 allows suction and injection of a microsample withprecision and operability comparable to oil hydraulic injectors. It issuitable for both injecting and holding during ICSI. Air bubbles donot enter the micropipette when the pneumatic microinjector isfitted.Movement range: 40mm* In the U.S.A., use IM-11-2A for clinical use.

Oil-type Microinjector IM-9B Pneumatic Injector IM-9CThe IM-9B, which is suitable for injection, and the IM-9C, which issuitable for holding, provide smoother and more stablemaneuverability by applying a new mechanism to minimizebacklashes. Also, their 53mm working distance, large-volumesyringe and just-the-right-size control knob with graduated scalesall contribute to easy control of the injection. The IM-9B has aglass syringe for easy confirmation of internal air bubbles while itsnew tube connector with a three-way stopcock makes for easierfilling of oil without generating air bubbles..

Pipette Holder Clamper HIK-5

The HIK-5 is used to keep the pipette holder at the desiredposition, thereby simplifying pipette exchange. The magneticclamper is attached to a bench or iron plate. Alternatively, itcan be attached to the included round metal plate, whichcan be affixed at the desired location to a microscope stageusing double-sided adhesive tape.

IM-9C

IM-9B

IM-11-2

IM-9B IM-9C

Movement distance

Movement range 53mm 53mm

Full rotation of knob 500µm 6mm

Control value

Full rotation of knob Approx. 10µl Approx. 480µl

Syringe 1,060µl glass type 4,240µl metal type

Manual 3D Micromanipulators

NMN-21/25The NMN-21 and -25 are designed for drift-free operation,making them ideal for delicate, patch-clamp research. The unithas no hydraulic system nor spring mechanism and its uniquedouble-slider structure minimizes vibrations transmitted from theoperator’s hand to the tip of the pipette via the control knob. Theunit’s motor-less and actuator-less design also eliminateselectromagnetic disturbances. The NMN-25 features a compact,space-saving design without a scale.

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Micromanipulators for electro-physiology applications

3D Motorized Micromanipulator

MM-80The MM-80 has been developed to satisfy the competing goals of"stability" and "compactness" that are essential for researchwork in electrophysiology.The motorized micromanipulator is constructed in a simplifieddesign with its controller employing simple-design push buttonswitches. The drive unit embodies user-friendly convenientfeatures which help reduce stress during experiments.

Movement range Fine 20mm (X, Y and Z axes), coarse 30mm (X, Y and Z axes)

Driving speed Minimum Approx. 10µm/s*(Motorized fine drive) Maximum Approx. 150µm/s*

Movement range Fine 2mm (X, Y and Z axes), coarse 30mm (X, Y and Z axes)

Full rotation of knob 50µm

Minimum graduation Fine 0.2µm

NMN-21 NMN-25

Movement Fine 6mm (X, Y and Z axes), Fine 6mm (X and Z axes), range coarse 15mm (X, Y and coarse 15mm (X and Z axes),

Z axes) combined coarse and fineworking range 15mm (Y axis)

Full rotation Fine 250µm, Fine 250µm (X and Z axes), of knob coarse approx. 4mm approx. 250µm (Y axis)

Minimum Fine 1µm —graduation

3D Water-Hydraulic Micromanipulator

MHW-3The MHW-3 is a hydraulic micromanipulator for patch clampingthat reduces unwanted drift due to temperature change. A cartridge with a one-to-five moving ratio enables fine controlmanipulation equivalent to an oil hydraulic manipulator. The drumtype control unit enables high linear repeatability.

MHW-3 with NN-L3 adapter, configured with patch amplifier

NMN-21

NMN-25

* DC motor drive, theoretical value

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Recommended combination for various applications

For ICSI (Intracytoplasmic Sperm Injection), ES cellinjection and nucleus transferThe microinjection system is dedicated to floating cell applications.The inner diameter of the pipette tip is more than 5µm. For theinjection of the sperm, embryo-stem cell and nucleus, a manualinjector is suitable as it accurately transmits the movement ofuser’s hand via the handle.

Product Unit Model name

Components

Inverted microscope 1 Ti-U NAMC set

3D hydraulic micromanipulator 1 NT-88-V3

Microinjector (injection side) 1 IM-9B

Oil-type microinjector (injection side) 1 IM-9C

Thermal plate warmer: ThermoPlate TP series 1 TP-108R05

Options for making pipettes

Micropipette puller 1 PC-10

Pipette grinder 1 EG-400

Microforge 1 MF-900

Glass capillaries 1 G-100

For injection into adherent cellsThe microinjection system for adherent cells incorporates a one-axis oil hydraulic micromanipulator—it moves in the direction of apipette—only on the injection side, since it does not require aninjector on the holding side. A motorized injector that canaccurately control the injection amount of DNA and fluorescencedyes is suitable.


Components

Inverted microscope 1 Ti-U DIC set

3D hydraulic micromanipulator (single-hand type) 1 NT-88-V3SH

Mounting adapter 1 NS-TEV3

1D hydraulic micromanipulator 1 MMO-220A

Motorized microinjector 1 IM-300

Thermal plate warmer: ThermoPlate TP series 1 TP-108R05



Glass capillaries 1 GD-1


Components

Stereoscopic microscope 1 SMZ18 FL set

Mechanical micromanipulator 1 MN-153

Mounting adapter 1 NN-V, NR-2

Motorized microinjector 1 IM-300



Glass capillaries 1 GD-1

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Single-cell patch clamping with invertedmicroscopeThis system is suitable for single-cell patch clamp experimentsusing an inverted microscope. Two units, one each for recordingand stimulating, are included. A water-hydraulic micromanipulatoreffective for general manipulations is suitable.


Components

Inverted microscope 1 Ti-U DIC set

Mounting adapter 1 NN-L3

3D water-hydraulic micromanipulator 2 MHW-3

Patch amplifier adapter* 1 AP-12A/L/N or AP-13-2



Microforge 1 MF-830

Glass capillaries 1 GD-1.2


* Please select an adapter that suits the brand of patch amplifier used.

Single-cell patch clamping with upright microscopeThis system is suitable for single-cell patch clamp experiments usingan upright microscope. Two units, one each for recording andstimulating, are included. A water-hydraulic micromanipulatoreffective for general manipulations is suitable.


Components

Upright microscope 1 FN1-FL-DIC set

Mounting adapter 1 NN-R


Patch amplifier adapter* 1 AP-12A/L/N or AP-13-2



Microforge 1 MF-830



* Please select an adapter that suits the brand of patch amplifier used.

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Manual patch clamping This system is suitable for patch clamp experiments on slice cellsusing an upright microscope. The two stages that incorporate newfunctions were developed especially for the FN1. One is independentfrom the microscope and the other, a manual XY stage, enables themicroscope’s observation field to be changed. Though three water-hydraulic micromanipulators are used in the picture, any number canbe selected.


Components

Upright microscope 1 FN1-FL-DIC set

Stage 1 ITS-FN1


Patch amplifier adapter* 2** AP-12A/L/N, AP-13-2



Microforge 1 MF-830



* Please select an adapter that suits the brand of patch amplifier used. ** Any one of the following combinations is possible: AP-12A/L/N x2, AP-13-2 x1,

AP-12A/L/N x1 + AP-13-2 x2

Manipulator/Patch amplifier adapter

Headstage maker and model name MHW-3 MM-80NMN-21NMN-25

MWS-1B MHW-4

AXONHS-2, HS-4, VG-2, VG-2A, CV-4, CV-5, CV-202 (CV-201AU) AP-12A AP-14A

CV-203BU, CV-7A/B, HS-9A AP-13-2 AP-13-3

HEKAEPC-7, EPC-8, EPC-9 AP-12L AP-14L

EPC-10 AP-13-2 AP-13-3

Nihon Kohden JZ-230J, JZ-240J, JZ-245J AP-12N AP-14N

Patch amplifier adapter

Compatibility of patch amplifier adapters

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Accessories

Micropipette Puller PC-10The PC-10 is used to pull the glass capillary vertically, utilizing thegravitational force of its own weight. It has two modes: a singlepull, pulling capillary at one stretch, and a double pull in whichthe setting is changed in mid-process. The versatile vertical pulltype is suitable for both injection purposes and patch clamping.

Glass Capillaries G-100, G-1, GD-1/1.2/1.5G-100 is a thin-walled glass capillary ideal for ICSI, while G-1 is forgeneral purpose.The GD series is commonly known as double tubing. These glasscapillaries contain an internal glass fiber (approx. 100 µm).The glass capillaries have been prewashed in an ultrasonic washingmachine.

Pipette Grinder EG-400The grinder with minimized irregular movements of the grindingplane and the microscope are combined. Precise grinding whileconfirming needle contact with grinding plane is facilitated.

Micropipette Puller PN-31The PN-31 uses a pulling method by which a glass capillary is fine-drawn using electromagnetic force, enabling the production oflong, sharp microneedles. The pulling tension can be adjustedover a wide range, from weak tension that is required at thebeginning of pulling to strong tension that is required to make along, thin microneedle.

Microforge MF-830A powerful microscope with 525x total magnification isincorporated to make electrodes with tips less than 2µm for patchclamping.

Microforge MF-900The MF-900 is designed to produce injection and holdingpipettes. In addition to the temperature and illumination, theposition of the heating element can be adjusted.

GD-1G-1G-100

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Thermal plate warmerThermoPlate TP seriesA temperature-controllable stage ring with a glass-heating plate ensures more accurate and reliable thermal control of specimens.The temperature can be set at between room temperature +5ºC and 50ºC in 0.1ºC increments. A sterilized sensor allows measurement ofthe actual temperature of dish contents. Management software and continuous current control provide solutions to a wide range ofrequirements.Manufactured by Tokai Hit Co., Ltd.

Simple Head Holder Plate (for Mice) MAG-1After affixing the chamber frame to the head of a mouse, theMAG-1 allows observations/experiments under the microscopeor other experimental instruments. Designed to be compact insize, the MAG-1 can be placed under a two-photon microscopeor a stereomicroscope.

For inverted microscope

MAG-1

Configuration with Stereo Microscope SMZ18

* Parts for conventional three-pointfixation can be customized.

For stereoscopic microscope

En

Micromanipulators and related equipment other than microscopes are manufactured by:

NARISHIGE CO., LTD. 27-9, Minamikarasuyama 4-chome, Setagaya-ku, Tokyo 157-0062, JapanTel: +81(0)3 3308 8233 Fax: +81(0)3 3308 2005e-mail: [email protected]://narishige-group.com

Printed in Japan (1309-04)T Code No. 2CE-MRRH-3 This brochure is printed on recycled paper made from 40% used material.

Photos courtesy of: Masumi Hirabayashi, Associate professor, National Institutefor Physiological Sciences

Specimen1: Injection of rat’s ES cell into 8-cell embryo

Specimen2: Microinsemination of rat egg

Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer. September 2013©1997-2013 NIKON CORPORATION

N.B. Export of the products* in this brochure is controlled under the Japanese Foreign Exchange and Foreign Trade Law. Appropriateexport procedure shall be required in case of export from Japan. *Products: Hardware and its technical information (including software)

WARNINGTO ENSURE CORRECT USAGE, READ THE CORRESPONDING MANUALSCAREFULLY BEFORE USING YOUR EQUIPMENT.

NIKON CORPORATIONShinagawa Intercity Tower C, 2-15-3, Konan, Minato-ku, Tokyo 108-6290, Japan phone: +81-3-6433-3705 fax: +81-3-6433-3785http://www.nikon.com/instruments/

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Micromanipulator System NT-88-V3 Series...The NT-88-V3 series, jointly developed by Nikon and Narishige, ensures easy operation and an unsurpassed level of precision. When used in