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EXPERIMENTS INCLUDED

MICROBIOLOGYSP-3008-MB-01

Microbiology-Rules for Success

Microorganisms, Aseptic Technique, and Cultures

Bacteria Morphology and Staining Techniques

Enumeration, Dilution, and Plate Counts

Kirby-Bauer Diffusion for Antibiotic Effectiveness

Hand Washing and Normal Flora

Food Safety

Environmental Influences on Microbial Growth-Salinity Testing

Microorganisms as Vectors of Disease

NUMBER OF EXPERIMENTS: 9

MICROBIOLOGY

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LabPaq Core satisfies the basic laboratory competencies required for general education

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» Utilizing an 8 experiment model, students gain exposure and experience with the core concepts and techniques required for continuing education and workforce development.

» With content tailored for student comprehension and success, each student is provided with a structured experience to build self confidence, critical thinking skills, and experimentation techniques.

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Experiment Summary

Microbiology-Rules for SuccessStudents review the time allocations for each experiment and the process for pouring agar plates. Each student will review the procedures for selecting an incubation location and then reflect on a series of helpful hints and tools for success.

Microorganisms, Aseptic Technique, and Cultures

Students will outline aseptic techniques and describe the safe handling of microbes. Students will identify media and plating techniques for various types of bacterial cultures. Each student will aseptically transfer microbes between media and observe microbial growth in broths and on agar plates.

Bacteria Morphology and Staining Techniques

Students will describe bacterial staining techniques and the differences between a Gram-positive and Gram-negative bacteria. Each student will prepare bacterial smears using direct, negative, and differential staining techniques.

Enumeration, Dilution, and Plate Counts

Students will describe how viable plate counts are performed and explain the role of dilution. Each student will create dilutions of microbe cultures and quantify the number of colonies on plates to determine the CFU/mL of the original culture.

Kirby-Bauer Diffusion for Antibiotic Effectiveness

Students will describe antibiotics and outline how penicillin, novobiocin, and gentamicin function. Each student will perform a Kirby-Bauer Diffusion test and compare microbial sensitivity to three antibiotics.

Hand Washing and Normal FloraStudents will describe normal and transient flora and explain proper hand washing techniques. Each student will culture microbes from washed and unwashed hands and relate experimental results to the effectiveness of hand washing.

Food SafetyStudents will describe common methods used to maintain food safety. Each student will culture microbes collected from fresh foods to compare food handling techniques and relate experimental results to methods for improving food safety in the home.

Environmental Influences on Microbial Growth-Salinity Testing

Students will describe the environmental conditions affecting microbial growth and explain osmolarity. Each student will perform salt tolerance testing on skin microbes and relate experimental results to conditions where these microbes occur.

Microorganisms as Vectors of Disease Students will define and identify types of diseases and pathogenic microorganisms. Each student will simulate the transmission of a microbe that is a vector of disease.

MICROBIOLOGY

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