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Manual
innuPREP PCRpure Kit
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Contents
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1 Safety precautions ......................................................................... 3
2 Storage conditions......................................................................... 3
3 Function testing and technical assistance................................... 3
4 Product use and warranty ............................................................. 3
5 Kit components .............................................................................. 4
6 General Scheme............................................................................. 4
7 Product specifications................................................................... 5
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8 Protocol: Purification and concentration of PCR products from
PCR reactions up to 50 µl .............................................................. 6
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9 Troubleshooting ............................................................................. 8
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1 Safety precautions
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2 Storage conditions
K@-&/ ++01231&1)2405-&! /,&. @*0>?& A-&. ,*5-?& ?5:J&8, &5 **D&, -D4-58,05-&L %MN&'#&O)PF&6 ,&/.&.,8A>-&9*5&8,&>-8.,&%'&D*+,@.&0+?-5&,@-.-&;*+?/,/*+.F&Q-9*5-&-H-5:&0.-&D8E-&. 05-&, @8,&8 >>&; *D4*+-+,.&@ 8H-&5 **D& ,-D4-58,05-F&6 9&, @-5-&85 -& 8+:&45-;/4/,8,-.&G /,@/+&, @-&45 *H/?-?&. *>0,/*+.&. *>H-&, @-.-&45 -;/4/,8,-.&A: &; 85-90>&G85D/+CF&
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3 Function testing and technical assistance
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4 Product use and warranty
K@-&0.-5&/.&5-.4*+./A>-&,*&H8>/?8,-&,@-&4-59*5D8+;-&*9&,@-&=+8>:,/E&R-+8&=S&E/,.&9*5&8+:&485,/;0>85&0.-J&./+;-&,@-&4-59*5D8+;-&;@858;,-5/.,/;.&*9&*05&E/,.&@8H-&+*,&A--+&H8>/?8,-?&9*5&8+:&.4-;/9/;&844>/;8,/*+F&=+8>:,/E&R-+8&=S&E/,.&D8:&A-&0.-?&/+&; >/+/;8>&?/ 8C+*.,/;&> 8A*58,*5:&. :.,-D.&8 9,-5&, @-&> 8A*58,*5:&@8. & H8>/?8,-?&, @-&;*D4>-,-&?/8C+*.,/;&.:.,-D&8.&5-U0/5-?&A:&)Z6=[&\\&5 -C0>8,/*+.&/+&,@-&]F"F&*5&-U0/H8>-+,.&/+&*,@-5&;*0+,5/-.F&
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!Note
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5 Kit components
!Important
",*5-&8>>&;*D4*+-+,.&8,&5**D&,-D4-58,05-F&
& 10 purifications 50 purifications 250 purifications
Binding Buffer %$&D>& #$&D>& '#$&D>&
Elution Buffer '&D>& #&D>& <$&D>&
Spin Filter LC5--+P %$& #$& #&B&#$&
Receiver Tubes %$& #$& #&B&#$&
Elution Tubes %$& #$& #&B&#$&
Manual %& %& %&
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6 General Scheme
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Addition of Binding Buffer to the PCR reaction mix
Binding of the PCR fragments onto Spin Filter (green)
Elution of the PCR fragments&
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&&
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Short operation manual – innuPREP PCRpure KitPuplication No.: HB_KS-5010_e_120116
1/ 2
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innuPREP PCRpure Kit
Protocol: Purification and concentration of PCR products
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Short operation manual – innuPREP PCRpure KitPuplication No.: HB_KS-5010_e_120116
2/ 2
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Expression and further use permitted with indication of source. © 2012 Analytik Jena AG, AJ Innuscreen GmbH
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7 Product specifications
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1. Starting material:&
1)2&5-8;,/*+&D/B,05-.&L04&,*&#$&_>P&
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2. Time for isolation:
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3. Fragment length:&
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4. Binding capcity:&
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5. Rate of recovery:
!& ($&N&X#&j&&
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8 Protocol: Purification and concentration of PCR products from PCR reactions up to 50 µl
!Important
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1. Binding of the PCR fragments
!& =??&500 µl Binding Buffer&,*&,@-&"4/+&V/>,-5F&
!& =??&04&,*&50 µl&*9&:*05&PCR reaction mixture&,*&,@-&"4/+&V/>,-5&LC5--+P&G@/;@&/.&8>5-8?:&45-d9/>>-?&G/,@&,@-&Q/+?/+C&Q099-5F&
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Alternatively
!& Mix 500 µl Binding Buffer&with up to 50 µl& *9&, @-& PCR sample& H-5:&G->>&A :&4/ 4-,,/+C&* 5&H*5,-B/+C&*0,./?-& ,@-&"4/+&V/>,-5&/+&8&.-4858,-&5-8;,/*+&,0A-F&
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Note:&=H*/?&8+:&;*+,8;,&*9&,@-&"4/+&V/>,-5&G/,@&,@-&9>*G&,@5*0C@F&&
2. Elution of the PCR fragments
!& 1>8;-&,@-&"4/+&V/>,-5&/+,*&8&3>0,/*+&K0A-F&
!& 1/4-,,-&at least&10 µl Elution Buffer&L*5&??e'$P&?/5-;,>:&*+,*&,@-&;-+,-5&*9&,@-&"4/+&V/>,-5F&
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!Important notes
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<F&69&,@-&H*>0D-&*9&,@-&1)2&5-8;,/*+&D/B&/.&@/C@-5&,@8+&#$&_>J&.4>/,&,@-&1)2&D /B& 8+?&8? ?&, *& -8;@&485 ,&#$ $& _>&Q /+?/+C&Q 099-5F&Z *8?&A* ,@&D/B-.&*+-&8 9,-5&8 +*,@-5&L .0;;-../H->:P&*+&, @-&" 4/+&V />,-5F&) -+,5/d90C-&, @-& 9/5.,&485,& 9*5& %&D /+0,-&8+?&?/ .;85?& ,@-& 9/>,58,-F&)-+,5/90C-&,@-&. -;*+?&485,&* 9&, @-&D /B&9 *5&'&D /+0,-.F& V*>>*G&+*G&, @-&-> 0,/*+&.,-4&8.&?-.;5/A-?&8A*H-F&
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9 Troubleshooting
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Problem / probable cause Comments and suggestions
Low recovery& &
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engl.&$%7%'&– Analytik Jena AG, Jena
Analytik Jena AG
Life Science
Konrad-Zuse-Strasse 1
07745 Jena / Germany
Phone +49 (0) 36 41 77 - 94 00
Fax +49 (0) 36 41 77 - 76 77 76
www.bio.analytik-jena.com
