MICRO TOTAL ANALYSIS SYSTEMS 2000

Micro Total Analysis Systems 2000

Proceedings of the fJTAS 2000 Symposium, held in Enschede, The N etherlands, 14-18 May 2000

edited by

A. VAN DEN BERG

W. OLTHUIS

and

P. BERGVELD MESA+ ·Research Institute, University of Twente, Enschede, The Netherlands

SPRINGER-SCIENCE+BUSINESS MEDIA, B.V.

A C.I.P. Catalogue record for this book is available from the Library of Congress.

ISBN 978-90-481-5496-8 ISBN 978-94-017-2264-3 (eBook)

DOI 10.1007/978-94-017-2264-3

Cover design: Martin van Es

Printedon acid-free paper

All Rights Reserved © 2000 Springer Science+-Business Media Dordrecht Originally published by Kluwer Academic Publishers in 2000

No part of the material protected by this copyright notice may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording or by any information storage and retrieval system, without written permission from the copyright owner.

The program committee and the organizers kindly thank the following sponsors:

MESA+ Analytical Chemistry

Microcosm Jenoptik

Hewlett Packard 3T

Cepheid Twente MicroProducts University of Twente

-JENOPTIK - JENA

Mikrotechnik

[he) ~!~K~J6 I Expanding Possibilities I

TMP

University of Twente The Netherlands

STEERING I PROGRAM COMMITTEE J.LTAS 2000

Albert van den Berg (Conference Chainnan) University of Twente MESA+ Research Institute

Y oshinobu Baba University of Tokushima Department of Medical Chemistry

Piet Bergveld University of Twente MESA+ Research Institute

GünterFuhr Humboldt Universität zu Berlin Institut für Biologie

D. Jed Harrison University of Alberta Department of Chemistry

Andreas Manz Imperial College London Department of Chemistry

M. Allen Northrup Lawrence Livermore National Labaratory Microtechnology Center

J. Michael Ramsey Oak Ridge National Labaratory Chemical and Analytical Seiences Division

Nico F. de Rooij Universite de Neuchiitel Institute de Microtechnique

Bart H. van der Schoot Universite de Neuchdtel Institute de Microtechnique

Shuichi Shoji Waseda University Department of Electronics, Information and Communication Engineering

Preface

Micro Total Analysis Systems 2000 (J..tTAS 2000) is the fourth international conference entirely dedicated to the newest scientific and technological developments in the field of miniaturized systems for (bio )chemical analysis and synthesis. Initiated in 1994 by Piet Bergveld and myself as an attempt to bring together in one workshop scientists with an analytical (bio )chemical background and microsystem researchers and engineers, the J..t T AS workshop series started in Enschede in 1994 with around 160 participants, began to grow in following meetings: Basel in 1996 (275 participants) and the very successful form er meeting in Banff in 1998 ( 420 participants ). These successes are a clear indication of the need for such a multidisciplinary meeting on the one hand, and perhaps, more important, an indication of the enormous commercial potential of "labs-on-a-chip", as such systems are frequently named. At the meeting of the steering committee during J..tTAS '98 in Banff, first of all it was decided to change the frequency of the meeting from biannual to annual via an interim period of three meetings spaced 18 months apart. Further, the decision was taken that quality rather than quantity would be the key-point for future ~TAS conferences, and that the single-session format would be maintained as much as possible. Given this fact, and the logistic constraints of the location at the University of Twente, for this meeting the program committee had to select 34 oral presentations and 100 posters out of over 230 submitted abstracts, implying an acceptance rate slightly below 60%. T ogether with the 12 excellent invited speakers, I believe that the program committee has realized a very high quality program, and I want to thank them all for their hard task ofreading through all the abstracts. Chapeau!

Compared to the earlier meetings there is one striking observation, which is that new trends appear to be impredictable! Array-systems, one of the highlights in Banff, have already entered the commercial domain, and research efforts seem to have diminished. Apart from genomic analysis there is now much focus on cell analysis. There is a lot of exciting research on centrifugal fluidic systems using plastic-CD devices as a disposable platform, not presented at the form er meeting. The merger of J.l T AS with microreaction devices into micro (bio )chemical systems seems to be an indication of a paradigm shift taking place not only in analytical or pharmaceutical chemistry, but in chemistry as a whole: why should one synthesize more than a couple of nanoliters of a certain compound if all necessary assays or screens can be made with that small amount? It is the information content that counts, and in that case miniaturization always pays off. Finally, it is amazing to see the innovation potential of the J..tTAS field: photoacoustic detection in microreactors, molecular emission detection on a chip, very high pressure microreactor devices and shear-flow driven Separations are just a couple of examples of the exciting novelties at this meeting.

This volume contains the manuscripts of all the presentations of j.tTAS 2000, starting with the oral presentations according to the conference program per day, followed by all of the related extended abstracts from the posters, arranged by topic as much as possible. In this way this book may serve the conference participants during the meeting and interested readers as textbook afterwards. Authors and subject indexes are added to facilitate consultation of references.

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Finally, I would like to express my great gratitude to the local orgamzmg committee, Piet Bergveld, Juergen Brugger, Martin van Es, Han Gardeniers, Hermine Knol, Jose Nijhuis, Wouter Olthuis and Janny Spierenburg for their assistance and initiatives in organizing this meeting. Further, I would like to thank J ed Harrison and Andreas Manz for their helpful informal comments and suggestions, and the former MESA-director Jan Fluitman who brought up the suggestion to start this workshop series almost 7 years ago. But of course the most important contribution to the success of this meeting comes from you, the participants! I wish you lots of excitement at this meeting from the oral and poster presentations in addition to the invaluable personal contacts. I am fully convinced that this proceedings may serve as a reference work at least until the next 1.1.TAS meeting!

Albert van den Berg f.I.TAS 2000 Chairman March 1, 2000

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CONTENTS Day 1

Flow Based Systems

Microflow Analysis based on Sequential and Bead Injection ................................................ 1 J. Ruzicka

Utilization of Bead based Reagents in Microfluidic Systems .............................................. 11 R.D. Oleschuk, A.B. Jemere, L.L. Schultz-Lockyear, F. Fajuyigbe and D.J Harrison

Analytical Devices based on Transverse Transport in Microchannels ................................ 15 P. Yager, C. Cabrera, A. Hatch, K. Hawkins, MR. Hall, A. Kamholz, K. Macounova and B.H. Weigel

Cell Analysis

Airborne Chemistry for Biological Micro Analysis ............................................................. 19 S. Nilsson, S. Santesson, E. Degerman, T. Johansson, T Laurell, J Nilsson and J Johansson

Single Organelle Analysis with Integrated Chip Electrophoresis and Optical Tweezers .... 25 A.R. Wheeler, K. Morishima, D. W. Arnold, A.B. Rossi and R.N. Zare

Separation of Target Microbe in Microchip by Flow Balancing and Visually Guided Laser Tele-Manipulator .................................................................................................................. 29

F. Arai, M Ogawa, A. Ichikawa, M. Deguchi, T Fukuda, K. Horio and K. Itoigawa

Microtechnology and Microreactors

Microreaction Technology for Process Intensification and High Throughput Screening .... 33 W. Ehrfeld, H.J Hartmann, V. Hesse!, S. Kiesewalterand H. Löwe

Micromachined fluidic couplers ........................................................................................... 41 E. Meng, S. Wu and Y-C. Tai

In-channel Processing to create Autonomaus Hydrogel Microvalves ................................. 45 R.H.Liu, Q. Yu, JM. Bauer, B.H. Jo, JS. Moore and D.JBeebe

Miniaturization and Integration ofPhotoacoustic Detection with a Microfabricated Chemical Reactor System .................................................................................................... 49

S.L. Firebaugh, K.F. Jensen and M.A. Schmidt

Microchemistry

Self-Assembled Monolayers with Chemical Functions ....................................................... 53 A. Frifferi, S. Flink and D. Reinhoudt

The Design of a Continuous Flow Combinatorial Screening Micro Reactor System with On-Chip Detection ............................................................................................................... 59

V. Skelton, G. Greenway, S. Haswell, P. Styring, D. Morgan, B. Warrington and S. Wong

lX

X

Miniature Tools for Combinatorial Chemistry ..................................................................... 63 C. Gonzalez, G. Maslana, J. Olson and J. Pan

Derivatization ofPlastic Microfluidic Devices with Polyelectrolyte Multilayers ............... 67 S.L. Barker, M.J. Tarlov, M. Branham, J. Xu, W MacGrehan, M. Gaitan and L.E. Locascio

Posters day 1

Flow-Through Chip Calorimeter based on BiSb/Sb-Thin-Film Thermopiles with a Thermopower of 64 m V IK ................................................................................................... 71

M Zieren, R. Willnauerand J.M. Köhler

Optimization ofthe Droplet Formation in a Piezoelectric Flow-Through Microdispenser. 75 J. Nilsson, J. Bergkvist and T Laurell

A Multi-Nozzle Piezoelectric Microdispenser for Improving the Dynamic Volumetrie Range ofDroplets ................................................................................................................. 79

M Stjernström, L. Rosengren, J. Holm, M Vangbo and S. Tormod

A Microfluidic-Based Nanoscope ........................................................................................ 83 S. Stone, C.D. Meinhart and S. Wereley

Design Analysis and 3D Measurement ofDiffusive Broadening in a Y-Mixer .................. 87 K.B. Greiner, M. Deshpande, J.R. Gilbert, R.F. Ismagilov, A.D. Stroock and G.M. Whitesides

Negative Dielectrophoresis Prevents Surfaces from Blocking ............................................ 91 G. Gastrock, S. Kunze, S. Howitz and J. Metze

A Va1ve-Less Diffuser Micropump For Microfluidic Analytical Systems .......................... 95 H. Andersson, W van der Wijngaart, P. Nilsson, P. Enoksson and G. Stemme

Biosystem for the Culture and Characterization ofEpithelial Cell Tissues ......................... 99 S. Hediger, A. Sayah, W Hunziker and M.A.M. Gijs

Somatic Cell Counting with Silicon Apertures .................................................................. 103 U.D. Larsen, H. Norring and P. Teileman

Microfabricated Fluidic Devices for Cellular Assays ........................................................ 107 SC. Jacobson, MA. McClain, C.T Culbertson and J.M Ramsey

Microbiology on-a-chip ...................................................................................................... 111 P.B. Monaghan, A. Manz and W. W. Niehofs

Dielectrophoretic Separation/Isolation of Rare Particles/Cell Types from a Heterogeneaus Suspension within a Microfluidic System .......................................................................... 115

D. Holmesand H. Morgan

Immuno-Electrophoresis ofBlood Cells on Micro-Capillary Chips .................................. 119 T lchiki, T Ujiie, T Okuda andY. Horiike

Realisation of a Cell Manipulation Bio-Microsystem using Shadow Mask Techniques ... 123 A. Tixier, Y. Mita, B. Le Pioujle, P. Surbled, Y. Murakami, E. Tamiya and H.Fujita

XI

A Combined Dielectrophoretic and Field-Flow Fractionation Microsystem for Biomedical Separation and Analysis ..................................................................................................... 127

J. Vykoukal, J. Yang, F.F. Becker, P.R.C. Gascoyne, P. Krulevitch, H. Ackler and J. Hamilton

Integrated Silicon Microheating Elements using Silicon-on-Plastic Drop In Functionality ............................................................................................................................................ 131

L.E. Locascio and M. Gaitan

A j.1TAS - Atomic Emission Flame Spectrometer (AES) .................................................. 135 S. Zimmermann, S. Wischhusen and J. Müller

Thermal Management and Surface Passivation of a Miniaturized PCR Device for Traditional Chinese Medicine ............................................................................................ 139

A. Lao, T. Lee, M. Carles and I.M. Hsing

jl-CE Chip Fabricated by Moving Mask Deep X-ray Lithography Technology ............... 143 0. Tabata, H. You, H. Shiraishi, H. Nakanishi, T. Nishimoto, K. Yamamato and Y Baba

Fabrication of Artificial Museie Based Valves for Controlled Drug Delivery .................. 147 M.J. Madau, KQ. He and A. Shenderava

Technology Chain for Polymer !l-T AS Applications ........................................................ 151 H. Becker, 0. Rötling, W. Röpke and U. Heim

Development ofMethods for On-line Chemical Detection with Liquid-Phase Microchemical Reactors using Conventional an Unconventional Techniques .................. 155

R.J. Jackman, T.M. Floyd, M.A. Schmidt and K.F. Jensen

Polymer Micro-Structures: Prototyping, Low-cost Mass Fabrication and Analytical Applications ........................................................................................................................ 159

J.S. Rassier, A. Schwarz, F. Bianchi, F. Reymand, R. Ferrigna and H.H. Girault

Integration of Micro-Optical Systems for Fluorescence Detection in !lTAS Applications 163 J. C. Raufet, HP. Herzig, E. Verpaarte, N.F. de Raaij and R. Dändliker

Micromachined Gas Chromatograph Based on a Plasma Po1ymerised Stationary Phase . 167 U. Lehmann, 0. Krusemark, J. Müller, A. Vogel and D. Binz

A Micromachined Electron Beam Ion Source ................................................................... 171 G. Petzald, P. Siebert and J. Müller

Finding a good Hydrogel Composition for Hydrogel based microvalves .......................... 175 H. van der Linden, W. Olthuis and P. Bergveld

Bonding and Etching Techniques for Precision Fabrication ofReliable Microvalves ...... 179 R.E. Oasterbroek, C.Q. Gui, J. W. Berenschot, S. Schlautman, T.S.J. Lammerink, M.C. Elwenspaek and A. van den Berg

Bi-directional Valve-Less Micropump Fabricated using Deep Reactive Ion Etching ....... 183 S. Matsumoto, A. Klein and R. Maeda

Diamond microchips for fast chromatography of proteins ................................................ 187 H. Björkman, C. Ericsson, S. Hjerten and K. Hjort

Microchamber Array for Immunosensor App lications ...................................................... 191 Y Murakami, T. Kikuchi, M. Yanase, H. Nagai, Y Morita and E. Tamiya

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Day2

Microfluidics I

The Decade's Search for the KillerAp in )l-TAS ............................................................... 195 D.J. Harrison, C. Wang, P. Thibeault, F. Ouchen and S.B. Cheng

Structurally Programmahle Microfluidic Systems ............................................................. 205 C.H Ahn, A. Puntambekar, S.M Lee, HJ Cho and C-C. Hang

Design and Simulation of Particles and Biomolecules Handling Micro Flow Cells with Three-Dimensional Sheath Flow ........................................................................................ 209

K. Tashiro, T Sekiguchi, S. Shoji, T Funatsu, W Masumoto and H Sato

Microfahricated Intrachannel Electrical Contacts for Material Transport Control.. .......... 213 J.M Ramsey, S.C. Jacobson, C.T Culbertson and R.S.Ramsey

Separation Systems

Microfahrication Technology for Chemical and Biochemical Microprocessors ............... 217 E. T Lagally, B.M. Paegel and R.A. Mathies

High Efficiency Separations on Microchip Devices .......................................................... 221 C. T Culbertson, S. C. Jacobson and JM Ramsey

An Integrated Fritless Column for Planar Capillary Electrochromatography with Conventional Stationary Phases ......................................................................................... 225

L. Ceriotti, E. Verpoorte and NF. de Rooij

Hand-held Miniature Chemical Analysis System (J.!ChemLah TM) for Detection of Trace Concentrations of Gas Phase Analytes ............................................................................... 229

G. Frye-Mason, R. Kottenstette, P. Lewis, E. Heller, R. Manginell, D. Adkins, G. Du/leck, D. Martinez, D. Sasaki, C. Mowry, C. Matzke and L. Anderson

Applications

Commercializing Lah-On-A-Chip Technology ................................................................. 233 A.R. Kopf-Sill

Centrifugal Microfluidics: Applications ............................................................................ 239 G.J Kellogg, TE. Arnold, B.L. Carvalho, D. C. Duffy and NF. Sheppard, Jr.

Versatile, Adaptahle and Programmahle Microfluidic Platforms for DNA Diagnostics and Drug Discovery Assays ...................................................................................................... 243

F. Pourahmadi, K. Loyd, G. Kovacs, R. Chang, M Taylor, S. Sakai, T Schafer, B. McMillan, K. Petersen and MA. Northrup

Integrated Cell Based Assays in Microfahricated Disposahle CD Devices ....................... 249 N Thomas, A. Ocklind, I Blikstad, S. Griffiths, M. Kenrick, H Derand, G. Ekstrand, C. Ellström, A. Larsson and P. Anderssan

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Single Moleeule and Single Cell Analysis

Microvolume Assays with Confocal Detection Systems ................................................... 253 R. Günther, R. Turner, D. Ullmann and G. Grad/

Living Cells in Opto-Electrical Cages-Characterisation, Manipulation and Force Measurements ..................................................................................................................... 261

G. Fuhr and C. Reichle

Microfabricated Multi-Frequency Particle Impedance Characterization System .............. 265 C. Fuller, J Hamiltan, H. Ackler, P. Krulevitch, B. Boser, A. Eldredge, F.F. Becker, J Yang and P. Gascoyne

Novel Separation Method on a Chip using Capillary Electrophoresis in Combination with Dielectrophoresis ................................................................................................................ 269

K. Morishima, D. W. Arnold, A.R. Wheeler, D.J. Rakestraw and R.N. Zare

Posters day 2

Flow Profiling in Microstructures with Micrometer Resolution ........................................ 273 J Holm, M. Gösch, H. Biom, T. Heino, P. Thyberg, G. Björk and R. Rigler

An Active Silicon Micromixer for j.tTAS Applications ..................................................... 277 P. Woias, K. Hauserand E. Yacoub-George

The Effect ofParticles on Perfonnance ofFixed-Valve Micropumps ............................... 283 L.S. lang, N.R. Sharma and F.K. Forster

Designing Corner Compensation for Electrophoresis in Compact Geometries ................. 287 JI. Mo/ho, A.E. Herr, B.Mosier, J G. Santiaga, T. W Kenny, R.A. Brennen and G.B. Gordon

Computer Simulations of E1ectrokinetic Sampie Manipulations in Microfluidic Devices 291 S. V. Ermakov, S. C. Jacobson and JM. Ramsey

Transport Number Mismatch Induced Stacking of Swept Sampie Zones for Microchip-based Sampie Concentration .............................................................................................. 295

Y. Liu, R.S. Faate, S.C. Jacabsan, R.S. Ramsey and J.M. Ramsey

Passive Microfluides- Ultra-Low-Cost Disposahle Lab-on-a-Chip Assays ...................... 299 B.H. Weigl, R. Bardell, T. Schulte and C. Williams

Dispersion Studies ofPressure Driven Flow in Deep Reactive-Ion Etched Microfluidic Channels ............................................................................................................................. 303

A. Daridon, P. Gravesen, H. Dirac, JP. Krog, E. Verpaarte and N.F. de Raoij

Combination of Sample Pre-Concentration and Capillary Electrophoresis On-Chip ........ 307 J Lichtenberg, A. Daridon, E. Verpaarte and N.F. de Raozj

Microfluidics in a Rotating CD .......................................................................................... 311 G. Ekstrand, C. Holmquist, A. Edma-Örlefors, B. Hellman, A. Larsson and P. Anderssan

Defect-oriented Testing ofMicroelectronic/Fluidic Systems ............................................ 315 H. G. Kerkhaff and R.E. Oosterbroek

XlV

A Microfluidic Sedimentation Particulate Capture Device with Interna! Degassing Membranes ......................................................................................................................... 319

MR. Hol/, K. Macounova and P. Yager

Self-Aligning Microfluidic Interconnects with Low Dead Volume .................................. 323 A. Puntambekar and C.H. Ahn

Development and Characterization of a Generic Microfluidic Subsystem toward Portable Biochemical Detection System .......................................................................................... 327

J. W Choi, CA. Wijayawardhana, N Okulan, A. Han, K. W Oh, S. Bhansali, V Govind, K. T. Schlueter, J.H. Nevin, WR. Heineman, H.B. Halsall, A.J. Helmicki, H. T. Henderson and C.H. Ahn

Ti02 Surface Modifications for Light Modulated Control of Flow Velocity .................... 331 C. Khoury, J. Moorthy, M. Stremler, J.S. Moore and D.J.Beebe

Polymer Microfluidic Valves, Membranesand Coatings ............................................. : .... 335 B.H. Jo, J. Moorthy and D.J.Beebe

Novel Designs for Electrokinetic Injection in JJTAS ......................................................... 339 M Deshpande, K.B. Greiner, J. West, J.R. Gilbert, L. Bousse and A. Minalla

Non-Destructive Mixing, Concentration, Fractionation and Separation of )Jm-sized Particles in Liquid by Ultrasound ..................................................................................................... 343

K. Yasuda

A Closed Loop Controlled Electrochemically Actuated Micro Dosing System for In-situ Sensor Calibration .............................................................................................................. 347

S. Böhm, B. Timmer, W. Olthuis and P. Bergveld

Consequences ofüpposing Electrokinetically and Pressure-Induced Flows in Microchannels ofVarying Geometries .............................................................................. 351

G.L. Lettieri, A. Dodge, G. Boer, J. Lichtenberg, E. Verpoorte and NF. de Rooij

On-Chip Separation ofExplosive Compounds -divided Reservoirs to Improve Reproducibility and Minimize Buffer Depletion ............................................................... 355

S.R. Wallenborg, C.G. Bailey and P.H. Paul

Counter Current Electrophoretic Sampie Preparation for Microscale Chemical Analysis 359 A.K. O'Keeffe, B.J. Treves Brown, P.R. Fielden, NJ. Goddard and R.D. Snook

Luminol Chemiluminescence Systems for Meta! Analysis by JJTAS ................................ 363 G.M. Greenway, L.J. Nelstrop, T. McGreedy and P. Greenwood

Miniaturized Capillary Isoelectric Focusing (ciEF): towards a Portable high-speed Separation Method ............................................................................................................. 367

A.E. Herr, J.I. Molho, J. G. Santiago, T. W. Kenny, D.A. Borkholder, G.J. Kintz, P. Belgrader and M.A. Northrup

Liquid/Liquid Extraction Systems using Micro-Contactor Arrays ................................... 371 J. Shaw, R. Nudd, B. Naik, C. Turner, D. Rudge, M. Bensan and A. Garman

A Cyclic Capillary Electrophoresis Separator on Silicon Substrate with Asynchronized Switching ............................................................................................................................ 375

Y.W. Jeong, B.H. Kim, J.Y. Lee, S.S. Park, M.S. Chun, K. Chun, B.G. Kim and D.S. Chung

XV

Microchip-Nano-Electrospray Device for Rapid On-chip Digestion and Mass Spectrometric Analysis ofHemoglobin Variants ...................................................................................... 379

I. M.Lazar, R.S. Ramsey and J.M. Ramsey

Capillary Electrophoresis with Direct and Contactless Conductivity Detection on a Polymer Microchip ........................................................................................................................... 383

G. Weber, M. Jöhnck, D. Siepe, A. Neyer and R. Hergenräder

Interfacing Protein and Peptideseparation to MALDI-TOF MS using Microdispensing and on-target enrichment Strategies .......................................................................................... 387

T. Miliotis, D. Ericsson, G. Marko- Varga, S. Ekström,J. Nilsson and T. Laurell

Miniaturized Capillary Electrophoresis System with Integrated Conductivity Detector. .. 391 R.B.M Schasfoort, R. Guijt-van Duin, S. Schlautmann, J. Frank, H. Billiet, G. W.K. van

Dedem and A. van den Berg

Microfabricated CE Chips with Optical Slit for UV Absorption Detection ...................... 395 T. Nishimoto, Y Fujiyama, H. Abe, M. Kanai, H. Nakanishi and A. Arai

Blood and Protein Separations Using a Micromachined Electrical Field-Flow Fractionation (~-EFFF) System ................................................................................................................ 399

B. Gale, K. Caldwell and A.B. Frazier

Glucose Measurement in Blood Serum Injected by Electroosmosis into Phospholipid Polymer Coated Microcapillary ......................................................................................... 403

A. Oki, S. Adachi, Y. Takamura, K. Ishihara, K. Kataoka, T. Ichiki and Y Horiike

Valveless, Sealed Microfluidic Device for Automated Heterogeneaus Assay: Design and Operational Considerations ................................................................................................ 407

A. Dodge, K. Fluri, V Linder, G.L. Lettieri, J. Lichtenberg, E. Verpaarte and N.F. de Rooij

On-chip Packed Channel Separations ................................................................................ 411 Y Fintschenko, SM Ngola, T.J. Shepodd and D. W. Arnold

Novel Injection Schemes for Ultra-High Speed DNA Separations ................................... 415 L. Bousse, A. Minalla and J. West

Realization of a Prototype Industrial On-line Sensing System for Ammoniumbasedon Micro System Technology: Results and Future Perspectives ............................................ 419

J.P. Krog, H. Dirac, B. Fabius, P. Gravesen, A. Daridon, J. Lichtenberg, E. Verpaarte, N.F. de Rooij, G. Pennarum- Thomas, M. Sequeira, D. Diamond, M. Denninger, 0. Geschke, J.P. Kutter, S. Howitz, C. Strec, P. Charles and L. Cognet

An Integrated System for Gene Detection Using Cycling Probe Technology ................... 423 M. Y Badal, T. Tang, W.E. Lee, T.D. Dickinson-Laing, D.E. Baderand D.J. Harrison

Single Moleeule Detection and Bead Trapping in Microstructures for Moleeule Selection and DNA Degradation ........................................................................................................ 427

M Gösch, Z. Földes-Papp, H. Biom, J. Holm, T. Heino, P. Thyberg, G. Björk and R. Rigler

Quantitative Design and Analysis of Single-particle Dielectrophoretic Traps .................. 431 J. Voldman, R.A. Braff, M. Toner, M.L. Gray and M.A. Schmidt

Zona Pellucida Removal ofMammalian Embryos in a Microfluidic System ................... 435 H.C. Zeringue, K.R. King, I.K. Glasgow, S. Raty, M.B. Wheeler and D.J. Beebe

XVl

Molecular Processing of a Single DNA Inside Channels for Genome Analysis ............... 439 K. Hirano, Y. Matsuzawa, H Yasuda, S. Katsura and A. Mizuno

New Micro Devices for Single Cell Analysis, Cell Sorting and Cloning-on-a-Chip: The Cytocon TM Instrument ........................................................................................................ 443

G. Grad!, T. Müller, A. Pfennig, S. Shirley, T. Schnelle and G. Fuhr

Day3

High-Throughput Experimentation

Synthesis and Analysis ofChemical Components in Nanoscale ....................................... 447 E. Litborn, M Curcio, A. Emmer and J. Roeraade

A Microsystem Platform Interfacing MALDI-TOF MS for High Speed Automated Protein Identification ...................................................................................................................... 455

S. Ekström, P. Önnerf}ord, M Bengtsson, T Miliotis, D. Ericsson, J. Nilsson, G. Marko-Varga and T. Laurell

High-Density Reagent Storage Arrays for High-Throughput Screening ........................... 459 S.A. Biondi, J.A. Wolk and A.R. Kopf-Sill

Towards Organic Synthesis in Microfluidic Devices: Multicomponent Reactions for the Construction of Compound Libraries ................................................................................. 463

M C. Mitchell, V. Spikmans, F. Bessoth, A. Manz and A. de Mello

DNA Analysis

Nanochip Technology for Single DNA Moleeule Analysis and Human Genomic Polymorphism Analysis ..................................................................................................... 467

Y. Baba

Micromachined Flow-Through Filter-Chamber for Solid Phase DNA Analysis ............... 4 73 H Andersson, A. Ahmadian, W. van der Wijngaart, P. Nilsson, P. Enoksson, M. Uhlen and G. Stemme

Parallel DNA Sequencing on Microfabricated Electrophoresis Chips .............................. 477 S. Liu, H Ren, Q. Gao, D.J. Roach, R. T. Lader Jr., T.M Armstrong, Q. Mao, I Blaga, D.L. Barkerand S.B. Jovanovich

Formation and Active Mixing ofMetered Nano/Picoliter Liquid Droplets in a Microfluidic Device ................................................................................................................................. 481

K. Hosokawa, T. Fujii and I Endo

Posters day 3

High throughput screening/residue analysis and high speed protein digestion using Miniaturized Membrane Reactor/Separator ....................................................................... 485

Y. Jiang, J. Gao, C.S. Lee, L.E. Locascio and M Gaitan

High-throughput Screening on Microchips ........................................................................ 489 A. Chow, A.R. Kopf-Sill, T. Nikiforov, A. Zhou, J. Coffin, G. Wada, L. Alajoki, M. Spaid, Y. Yurkovetsky, S. Sundberg and J. W Parce

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Multi Micro Reactors Consisting oflndividually Temperature Contralied Silicon Well Arrays Realizing Efficient Biochemical Reactions ............................................................ 493

K. Akahori, S. Kondo, E. Shinohara, K. Tashiro and S. Shoji

Plastic Microchip Electrophoresis for Clinical Applications of DNA Analysis ................ 497 S.H. Chen, YH. Chen, W.C. Sung, G.B. Lee, K.C. Young and T.T. Chang

DNA Preparation by using a DNA Chip ............................................................................ 501 K. Okano, G. Chen, K. Yasuda and S. Ishiwata

Thermal Gradient DNA Chip ............................................................................................. 505 T. Kajiyama, K. Murakawa and Y. Miyahara

Enzyme-Based Electrochemical Biosensor with DNA Array Chip ................................... 509 J. Gau, E.H. Lan, B. Dunn and C.M. Ho

Integration of Immunosorbent assay system into a multichannel Microchip for clinical diagnoses ............................................................................................................................ 513

K. Sato, M. Tokeshi, T. Odake, H. Kimura, T. Goi, M. Nakao and T. Kitamari

A Microbead Sieve and Polaragraphie Detection Cell for Immunoassays with a Modular Microfluidic Interconnect ................................................................................................... 517

R.B. Darling, E. Aw and M. Mar

High-Throughput SNP Scoring in a Disposahle Microfabricated CD Device ................... 521 A. Eckersten, A. Edma-Örlefors, C. Ellström, K. Erickson, E. Löfman, A. Eriksson, S. Eriksson, A. Jorsback, N Tooke, H. Derand, G. Ekstrand, J Engström, A.K. Honerud, A. Aksberg, H. Hedsten, L. Rosengren, M Stjernström, T. Hultman and P. Anderssan

Real-time PCR Analysis on Nucleic Acids Purified from Plasma using a Silicon Chip ... 525 P. Be/grader, R. Joshi, J. Ching, S. Zaner, D.A. Barkholderand MA. Northrup

Examination ofDielectrophoretic Behavior ofDNA as a Function ofFrequency from 30Hz to lMHz Using a Flexible Microfluidic Test Apparatus .................................................... 529

S.M. Crippen, M.R. Hol! and D.R. Meldrum

Multichannel Microchip System for Rapid Calibration and Immunoassay ....................... 533 S.B. Cheng, CD. Skinner, J. Taylor, W.E. Lee, M. Jolivet, G. Picelli and D.J. Harrison

mRNA Isolation for cDNA Library Construction on a Chip ............................................. 537 G. Jiang and D.J. Harrison

Sub-microliter Assays and DNA Analysis on Plastic Microfluidics ................................. 541 T.D. Boone, A.J. Ricco, P. Gooding, T. 0. Björnson, S. Singh, V. Xiao, I. Gibbons, S.J Williams and H. Tan

A Miniaturized and Integrated Plastic Thermal Chemical Reactor for Genetic Analysis . 545 H. Yu, P. Sethu, T. Chan, N Kroutchinina, J Blackwell, C.H. Mastrangelo and P. Grodzinski

Nanofabricated Device for Fluorescence Correlation Spectroscopy in Sub-Femtoliter Volumes .............................................................................................................................. 549

M.E. Foquet, S. W. Turner, J Kor/ach, W. W. Webband H.G. Craighead

Protein Microassays for Monitaring of Structural Changes of Proteins via Surface Enhanced Metal Nano Cluster Resonance ......................................................................... 553

C. Mayer, R. Palkovits, G. Baure and T. Schalkhammer

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Miniaturized Electrospraying as a Technique for the Production ofMicroarrays of Reproducible Micrometer Sized Protein Spots .................................................................. 557

R. Moerman, J Frank, J C.M Marijnissen, T. Schalkhammer and G. WK. van Dedem

Computational Simulation ofBio-Microfluidic Processes in Integrated DNA Biochips .. 561 A. Przekwas, V. Makhijani, M. Athavale, A.Klein and P. Barfeh

Day4

Microfluidics II

A Centrifugal Microfluidic Platform- A Comparison ....................................................... 565 · M.J. Madou, Y. Lu, S. Lai, J Lee and S. Daunert

An AC Magnetohydrodynamic Microfluidic Switch ......................................................... 571 A. V. Lernoff and A.P. Lee

Hydrofobic Valves by Ink-Jet Printing on Plastic CDs with Integrated Microfluidics ...... 575 A.L. Tiensuu, 0. Öhman, L. Lundbladh and 0. Larsson

Design Analyses of Capillary Burst Valves in Centrifugal Microfluidics ......................... 579 J Zeng, D. Banerjee, M. Deshpande, JR. Gilbert, D. C. Duffy and G.J. Kellogg

New Concepts

Electro-Kinetic-Pump Application in Micro-Total Analysis Systems: Mechanical Actuation to HPLC .............................................................................................................................. 583

P.H. Paul, D. W Arnold, D. W Neyer and K.B. Smith

An Atmospheric Pressure Plasma on a Chip Applied as a Molecular Emission Detector in Gas Chromatography .......................................................................................................... 591

J C. T. Eijkel, H. Stoeri and A. Manz

A Micro Viscosity Detector for a Planar Hydrodynamic Chromatography (HDC) System ............................................................................................................................................ 595

F.H.J van der Heyden, MT. Biom, J.G.E. Gardeniers, E. Chmela, MC. Elwenspoek, R. Tijssen and A. van den Berg

Shear-driven chromatography: the route towards pressure drop-less Chromatographie separations in nanometric channels .................................................................................... 599

G. Desmet, N. Vervoort and G. V. Baron

Posters day 4

Shah Convolution Fourier Transform Detection ................................................................ 603 Y.C. KwokandA. Manz

Macromolecular Crystallization Microarrayed Chip for Super Saturated Vapor and Liquid-Liquid Diffusion Screening ................................................................................................ 607

K. Akioka, S. Inoue, A. Sanjoh, T. Tsukihara and S. Gorti

High Pressure Gas-Liquid Mixtures Generated in a Micro-Electrolysis Cell. ................... 611 S. Böhm, H van der Linden, A. van den Berg, W Olthuis and P. Bergveld

XlX

Authors index ....................................................................................... 615

Subject index ........................................................................................ 621