Methods:Centrifugation:20 Grams of refrigerated cauliflower was obtained and ground up with 40mL of Mannitol Grinding Buffer and then filtered. 2mL of this solution was separated as F1 and the remaining was centrifuged (100x gravity for 30 min. at 4o C). Two distinct layers were created after centrifugation (pellet 1 or P1 and supernatant 1 or S1). P1 was placed in ice with 8.0 mL of Mannitol Assay Buffer. 2mL of S1 was stored and the rest was centrifuged (10,000 x gravity for 30 min. at 4oC). After centrifugation, S1 formed 2 layers (pellet 2 or P2 and supernatant 2 or S2). 8.0 mL of Mannitol Assay Buffer was added to P2Microscopy:One drop of each fraction (F1, S1, P1, S2, P2) was placed on the slide with 1 or 2 drops of Azure C dye and observed under the microscope. Pictures were taken of the observations.Succinate Dehydrogenase (Part 1):9 different cuvettes were obtained and each cuvette was filled with different concentrations of various solutions (Assay Buffer, Azide, DCIP, Malonate, and Succinate). 3 of the 9 were control cuvettes (Malonate, Succinate, and Azide). The Spectrophotometer was set to 600 nm wavelength and each of the cuvette’s OD/ absorbance was measured after the addition of 0.36 mL of the fraction samples. The OD was read at 0 and 3 minutes intervals of the addition of the fraction sample to each cuvette. (Part 2):10 new cuvettes were obtained and various concentrations of Mannitol Assay buffer were added to each cuvette (P2, P2A, P2B, P2C, & S2). Then 0.2 mL of DCIP and 0.2mL of Succinate were added to all the cuvettes. The Spectrophotometer was set at 600 nm wavelength and the absorbance values were observed for each of the cuvette after 0 and 3 minutes of adding the fraction samples from P2.