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Measuring the Effects of Intensive Forest Management on Innate ImmunityWhole blood bactericidal activity in wild deer mice (Peromyscus
maniculatus) in Oregon.
Natalie RogersWith the help of:
Dr. Rhea Hanselmann
In the lab of:Dr. Anna Jolles
Department of Biomedical Sciences
Background:Ecosystem erosion
e.g. intensive forest management (IFM)
Health suffers
Change in community compositio
n
http://sogweb.sog.unc.edu/blogs/localgovt/?attachment_id=5246http://www.topboxdesign.com/urban-development-project-in-shibam-yemen/urban-development-project-landscape-architecture/
Background (Cont.)
ImmunocompetenceInnate immune systemThe wild deer mouse (Peromyscus
maniculatus)Loss of habitat and stressors can impact this
species’ ability to fight pathogensDisease transmission
HypothesisInnate immunity in wild deer mice can
be negatively impacted by intensive forest management practices.
Intensive forest management erodes
ecosystem
Change in community composition
Innate immunity impaired
ObjectiveTo assess the innate immune system in wild deer mice (P. maniculatus) inhabiting intensely managed forestry sites in the Oregon coast range by measuring the bactericidal activity of whole blood.
http://pixdaus.com/?sort=tag&tag=deer%20mouse
MethodsLocation:
Two sites Six plots/site
Older stands Clearcut control Intensively treated
Trapping:Sherman live traps~180 traps/site
http://maps.google.com
Site 1
Site 2
Methods (cont.)
Whole blood bactericidal assay (BCA)Escherichia coli + Whole Blood + Medium →
Agar
Bacterial killing capacity (BKC) =(# of colonies on control plates)-(# of colonies on dilution
plates)(# of colonies on control plates)
Predicted ResultsBKC of wild deer mice will differ by management level:
Older Stands:High BKC
Control:
?Treatment:Low BKC
Results – What was Caught?
0
5
10
15
20
25
Older Stands Control
Treatment
Management Levels
Nu
mb
er
of
Deer
Mic
e
http://www.nature.com/nature/journal/v472/n7344/full/472392a.html
Results: How does IFM affect innate immunity?
0.65
0.7
0.75
0.8
0.85
0.9
1:23 Dilution
Management Level
BK
C
OlderStands Control Treatment
DiscussionHow do the different management levels
affect BKC?
What could cause this? Herbicides, resource availability, territoriality, etc.
0.65
0.7
0.75
0.8
0.85
0.9
1:23 Dilution
Management Level
BK
C
Older Stands Control Treatment
DiscussionOther dilutions show reversed trend:
Could be caused by confounding variables parasites, disease status, physiology etc.
1:471:110
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
BK
C
Management Levels
OlderStands
OlderStandsControl ControlTreatment Treatment
ConclusionsTrend shows management may affect BKC at
1:23 dilution.
This trend is reversed for lower and higher dilutions.
Data is preliminary.
Ecosystems can have effect on health and immunity, but in which way remains unclear.
Special thanks to:Howard Hughes Medical Institute
Department of Biomedical Sciences
Dr. Rhea HanselmannDr. Anna Jolles
Dr. Kevin Ahern
Jolles lab field crew
Thank you for the opportunity!