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Presentation designed for laboratory staff with computers that would not display Flash. Used PowerPoint to create an eLearning module on how to operate the pH meter.
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Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 1
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 2
Orion 3 Star Plus pH Meter
Front of Meter & Keypad
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 3
Start Up• Turn Printer ON• Press POWER Key on
Meter Display will read:
Star PLUS Software Version and
current method
Header information will print:
Meter Serial Number Meter Software Version Method currently set
• Check print quality
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 4
Calibrate Method 1
Select Method (# decimals) to be used for samples
Press MEASURE to set method
Open Fill Hole
Rinse electrode and blot carefully
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 5
Calibrate Method 1
Press CALIBRATE
CAL 1 Displayed..
Immerse electrode in first buffer
• pH will stop flashing when reading stable
• Buffer value will be displayed with leading zeroes
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 6
Calibrate Method 1After pressing CALIBRATE, • CAL 2 displayed• pH flashes while reading in progress
• Remove electrode from first buffer, rinse & blot
• Immerse electrode in second buffer
• pH will stop flashing when reading stable
• Buffer value will be displayed with leading zeroes
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 7
Calibrate Method 1
After pressing CALIBRATE, • CAL 3 displayed• pH flashes while reading in progress
• Remove electrode from second buffer, rinse & blot
• Immerse electrode in third buffer
• pH will stop flashing when reading stable
• Buffer value will be displayed with leading zeroes
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 8
Completing CalibrationPress MEASURE to print & save calibration results
• Check date and time (not visible in photo)
• Check mV for each buffer
• Check slope 1 and slope 2
• Initial & date printout
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 9
Verifying Instrument Performance• Remove electrode from buffer, rinse & blot
• Immerse electrode in QC (6.86) bufferPress MEASURE
pH will stop flashing when reading stable
Press MEASURE to print result
• Initial & date• Identify sample as QC Buffer
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 10
Documenting Calibration
• Check Dates & Times• Check Method• Check Calibration #• Check Limits:
• 4.01 +177 mV ± 30 mV
• 7.00 0 mV ± 30 mV
• 10.01 -177 mV ± 30 mV• Slope 1 and 2:
95% - 102%• QC Buffer: 6.86 ± 0.05 Method 1: 6.8 – 6.9 Method 2: 6.81 – 6.91
Calibration & Initial QC Buffer on form
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 11
Measuring Sample pH• Remove electrode from buffer or storage solution, rinse & blot
• Immerse electrode in samplePress MEASURE
pH will stop flashing when reading stable
Press MEASURE again to print result
• Initial & date• Identify sample
Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 12
Reviewing Results• Check Dates & Times
• If > 2 hours since last QC Buffer run, instrument performance must be verified by running the QC Buffer as a Batch control
• Check Method• 1 decimal for any product QC and most
reagents/solution• 2 decimals if reagent procedure
requires more sensitivity• NO ROUNDING
• Check Calibration #• Match to the most recent calibration for
the method selected
• Attach product results to Product QC Forms
• Attach reagent/solution pH measurements to Reagent pH Log [19.4.frm024]