MCF10A Her2miNS miRB

DAPI

RB

Supplemental Figure 1

ErbB2

RB

SKBR

3

BT47

4

Mcf1

0a/E

rbB2

MDA-M

B-23

1

Actin

Immunoblotting was performed in the indicated cell lines for the levelsof RB and ErbB2. Immunofluorescence staining of RB confirmed a uniform attenuation of RB protein levels throughout cultures with miRB.

Supplemental Figure 2

Invasion of MCF10A ErbB2 overexpressing cells was measured by modified Boyden chamber assays. The indicated cell lines were treated with AKT (GDC/NVP-BEZ) or ERK inhibitors (AZD/U0126) and invasion was scored as described in the materials and methods