Max Showalter7. June 2012Purdue University

Let’s begin at the parts registry homepage

Click On

When you get to this webpage, you will see several different icons you can click on.

The first thing you’ll notice on this page is a long list of Promoters, Ribosome Binding Sites, etc…

Further down, you will see that you can browse parts by part, type, function, or chassis

From this page and the search bar in the top right your job will be to find the pieces for a functional device.

If I’m going to request a part for my iGEM Team… What email address do I send my

request to? What do I include?

If I make a BioBrick part: What will the suffix be? What will the suffix be if I use Spe1 to

cut my plasmid? What temperature should I do my

digestion if I am using Spe1?

Find the part(s) that stopped transcription in the banana smell generating device (You will have to find the banana smell generating device first!) What are these parts called? What are their part registry numbers? How many base pairs does each have?

Find a constitutive eukaryote promoter that works and has a registry star

Find a Kozak sequence for yeast that works and has a registry star

A protein that will make your yeast fluoresce cyan – [Cyan Fluorescent Protein]

A terminator that works in yeast

(What is the name of the Chassis for these

All Biological Circuits must have:1.Promoter2.Ribosome Binding Site (RBS)3.Coding Region4.Terminator

Rules to the Game

Use Only:1.Parts that work 2.Parts that are availableSpecify a chassis and ensure your parts match this chassis

Now It’s Time to Assemble a Part

Let’s Make a Metal Sensor..

Metal Ions

Fe

Fe

Fe

Device

Assemble a list of the parts required for the device

Follow all the rules given

Team ADevelop two

systems for E. coli that will:

1. Make the bacteria fluoresce cyan

2. Make the bacteria smell like winter mint in the presence of salicylic acid

Team BDevelop two different systems for S. cerevisiae that will1.Make the yeast fluoresce Yellow2.Make the yeast fluoresce orange

As a bonus: yeast (a eukaryote) doesn’t use a ribosome binding site in the same sense as prokaryotes. What is the analogous part in yeast? Describe how it is like an RBS.

Make a double tiered system that will turn off color when banana smell has been turned on