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Magnetic Resonance Imagingfor Pre-clinical Applicationspplications
2
Applications
Brain Diffusion Tensor Imaging at 9.4T
Data courtesy: Laboratory of Functional and Metabolic Imaging,
Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
k-t SENSE Cardiac Imaging at 9.4T
Data Courtesy: CABI, University College London,
and BIC Imperial College London, UK
Sodium MRI of Kidney at 9.4T
Data Courtesy: Indiana University School of Medicine
Agilent’s range of MRI systems are used in a variety of
applications. Each system is carefully confi gured to meet
your requirements and your demands, while offering the best
performance of that system.
Some of the pre-clinical applications include:
• Brain and organ imaging
• Cardiac investigation
• Tumour assessment
• Investigation of contrast agents
• Magnetic resonance spectroscopy
We understand that data and image acquisition can be time
consuming and labor intensive. Therefore, our systems are
designed to improve throughput, increase effi ciency and
improve accuracy, allowing you to collect high quality data.
9.4T (above) and 14.1T (right) Brain Spectroscopy
Data courtesy: Laboratory of Functional and Metabolic
Imaging, Ecole Polytechnique Fédérale de Lausanne,
Lausanne, Switzerland.
3
Complete, Flexible MRI Systems
Agilent offers a complete range of pre-clinical MRI systems.
Each Agilent MRI system includes the DD2 console, a
high fi eld or ultra-high fi eld magnet, gradient and RF coils,
VnmrJ 3.1 software, and a selection of sample handling
options to meet your specifi c needs.
Working together with a range of clients, Agilent is able to
produce high fi eld magnetic systems exhibiting very high
stability and consistency. The architecture of the Agilent DD2
console allows superior performance on multiple channels.
Our VnmrJ 3.1 Software supports an extensive library of 2D,
3D and advanced MRI pulse sequences.
Data courtesy: Center for Magnetic Resonance Research, University of Minnesota,
Minneapolis, MN, USA.
4
High Field MRS4.7-9.4T Horizontal Bore Magnet Systems
9.4T/310mm
7T/210mm
Specifi cations for Horizontal Bore Magnet
Product
MRBR MRBR MRBR MRBR MRBR MRBR MRBR MRBR MRBR
4.7T/310 7.0T/160 7.0T/210 7.0T/310 7.0T/400 9.4T/160 9.4T/210 9.4T/310 9.4T/400
ASR ASR ASR ASR ASR ASR ASR ASR ASR
Operating fi eld (T) 4.7 7 7 7 7 9.4 9.4 9.4 9.4
Bore Size excl RT shim and Gradients (mm)
310 160 210 310 400 160 210 310 400
Homogeneity volume (mm DSV) 150 80 80 140 200 80 80 140 200
Homogeneity: fully shimmed peak to peak (ppm) / (mm) DSV
<±5ppm / 150mm
±2ppm / 80mm
±2ppm / 80mm
±2.5ppm / 150mm
±2.5ppm / 200mm
±2ppm / 80mm
±2ppm / 80mm
±2.5ppm / 140mm
±2.5ppm / 200mm
Homogeneity: superconducting only peak to peak (ppm) / mm DSV
±5ppm / 80mm
±4ppm / 80mm
±10ppm / 150mm
±10ppm / 200mm
±4ppm / 80mm
±4ppm / 80mm
±10ppm / 140mm
<10ppm / 200mm
System length (mm) 1280 1012 1280 1636 1998 1224 1420 1704 2286
Minimum ceiling height (mm) 3150 3130 2485 3030 3033 3300 3030 2990 3530
System diameter (mm) 1360 1350 1250 1655 2171 1500 1655 1740 2708
Zero boil-off Yes Yes Yes Yes Yes Yes Yes Yes Yes
Fringe fi eld (5 Gauss)(Axial x Radial) (m)
2.3 x 1.5 2.1 x 1.1 1.5 x 1.4 2.6 x 1.2 4.2 x 2.3 2.4 x 2.0 3 x 2 3.6 x 2.2 5 x 3.6
Pre-Clinical MRI at its Best
High Field MRI Systems
Available in a range of bore sizes from 160-900mm and in
fi eld strengths from 4.7-9.4T, Agilent's high fi eld magnets
are renowned for their market leading performance. Most
systems are available with active shielding technology. The
proton operating frequency of each system is dependent upon
fi eld strength.
The core products in the range comprise two MRI systems.
The Agilent MRI System is an adaptable MR imaging platform
that can be utilized in many MRI applications well as in the
development of novel research processes.
The Discovery MR901 System is a complete pre-clinical MRI
system operating on a clinical environment interface facili-
tated by GE Healthcare.
5
Ultra High Field MRI11.7-18.8T Horizontal Bore Magnet Systems
11.74T/160mm
16.4T/260mm
Meeting our Customers’ Needs
Agilent’s Ultra High Field MRI magnets adopt the same high
standards and practices as the high fi eld magnets. In fi eld
strengths of 11.7T to 18.8T, and with bore sizes 160-400mm,
these cutting-edge designs take into account every aspect
of a client’s needs, including ease of use, running cost and
space constraints.
We incorporate market-leading superconducting technology
to meet even the most demanding requirements and techno-
logical specifi cations.
And with each one being built for individual purposes and
customer requirements, you can be confi dent of superb MRI
performance every time.
Specifi cations for UHF Horizontal Bore Magnet Systems
Product
MRBR MRBR MRBR MRBR MRBR MRBR MRBR MRBR
11.7/160 11.7/210 11.7/310 11.7/400 14.1/260 16.4/260 17.6/210 18.8/210
Active Active Passive Passive Passive Passive Passive Passive
Operating fi eld (T) 11.7 11.7 11.7 11.7 14.1 16.4 17.6 18.8
Operating temperature (K) 4.2 2.3 2.3 2.3 2.3 2.3 2.3 2.3
Bore Size excl RT shim and Gradients (mm) 160 210 310 400 260 260 210 210
Homogeneity volume (mm DSV) 80 100 150 200 130 100 100 100
Homogeneity fully shimmedpeak to peak (ppm /mm DSV)
±2.5ppm / 80mm
±2.5ppm / 100mm
±2.5ppm / 150mm
±2.5ppm / 200mm
±2.5ppm / 130mm
±4ppm / 100mm
±2.5ppm /100mm
±2.5ppm /100mm
Homogeneity super conductingonly peak to peak (ppm /mm DSV)
±10ppm / 80mm
±10ppm / 100mm
±5ppm /100mm
±5ppm /100mm
±10ppm /130mm
±10ppm / 100mm
±5ppm /100mm
±5ppm /100mm
Minimum Hold Time between Helium refi lls (days) 365 365 50 50 50 50 32 32
Minimum Hold Time between Nitrogen refi lls (days) N/A N/A 8 8 14 14 14 14
System length (mm) 1400 1680 2240 2600 2132 2572 2572 2920
Minimum ceiling height (mm) 2950 3030
System diameter (mm) 1840 1690 2100 2380 1820 2100 2100 2380
In vivo brain phase imaging at 14.1T
Data courtesy: Laboratory of Functional and Metabolic Imaging,
Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
6
Gradient CoilsHigh Duty Cycle Gradient Coils
Nested Gradients
Agilent 205/120 HD gradient,
including cutaway 3D CAD
Agilent’s latest generation of high performance gradients has
been designed and developed by MR scientists to address the
most challenging techniques and applications at the highest
magnetic fi elds.
Features include:
• Excellent heat extraction, providing industry-leading
high duty cycle performance
• Improved peak strength with short rise times
• Microgroove technology for superior magnetic shielding
• High slew-rates
• Superior gradient linearity
• High strength room-temperature shims
• HD 305/210 and HD 205/210 now rated to 300A peak
current providing increased gradient strength performance
Specifi cations for Gradients
Outside diameter: 395mm 305mm 205mm 156mm 156mm 115mm
Inside diameter: 290mm 210mm 120mm 100mm 90mm 60mm
Peak Current: 300A 300A 300A 200A 200A 200A
Peak Voltage: 500V 500V 500V 300V 300V 300V
Gradient sensitivity: 0.333mT/m/A 1.0mT/m/A 2.0mT/m/A 2mT/m/A 3.75mT/m/A 5.0mT/m/A
Maximum gradient strength: 100mT/m 300mT/m 600mT/m* 400mT/m 750mT/m* 1000mT/m*
Maximum slew rate: >380T/m/s >700T/m/s >4444T/m/s >3000T/m/s >5770T/m/s >7690T/m/s
Maximum DC current in all 3 axes simultaneously, I
DC Max
100A 75A 75A 55A 45A 50A
Duty cycle @ IMax
: 11.1% 6.25% 6.25% 7.6% 5.1% 6.25%
Minimum inductive rise time: 162μsec 229μsec 38μsec 50μsec 54μsec 16μsec
Gradient sub-system rise time: 260μsec 425μsec 135μsec 130μsec 130μsec 130μsec
Linearity (% over DSV): <5 %/200mm <5 %/120mm <5 %/80mm <5 %/75mm <5 %/60mm <5 %/40mm
Number of shims: 9 (including gradient shims)
Shim strengths:
Z0 348mG/A 380mG/A 530mG/A 470mG/A 505mG/A 510mG/A
Z2 16.6mG/cm2/A 29.7mG/cm2/A 87mG/cm2/A 89.7mG/cm2/A 127mG/cm2/A 157mG/cm2/A
ZX, ZY 5.1mG/cm2/A 12.2mG/cm2/A 41mG/cm2/A 62mG/cm2/A 73.5mG/cm2/A 124mG/cm2/A
2XY, X2-Y2 2.5mG/cm2/A 5.6mG/cm2/A 12.7mG/cm2/A 16.8mG/cm2/A 23.5mG/cm2/A 40mG/cm2/A
Shim algorithm 3D Automatic
Shim algorithm Manual Interactive
7
A key feature of Agilent’s complete range of RF coils is the
high level of RF homogeneity and stability, which is vital to
effective imaging, whether in the transmit or receive phase,
while maintaining excellent signal to noise ratios.
We have an extensive catalogue of RF coils, which fall into
fi ve key categories central to MRI applications. These are:
• Millipede: Suitable for whole body scanning and micro-
imaging, these coils produce consistent imaging with
reduced potential systematic errors
RF Coils
• Volume: suitable for all pre-clinical applications, the sample
fi ts fully inside the coil, minimizing the distance between
the coil body and the sample surface.
• Surface: ideal for oncology, surface coils enable increased
signal-to-noise ratios.
• Phased Array: ideal for neurological, spinal and cardiac
imaging, these coils are available in a variety of anatomical
spatial arrangements.
• Dual-tuned: these can increase your productivity by a signif-
icant amount, because they capture data at two different
frequencies at the same time. Volume and surface coils are
available in dual-tuned format.
For Every Application
Selection of Phased Array RF Coils and support devices.
Millipede RF Coil
8
Sample HandlingSample Positioning System
Cart Option
SPS 60 for use with the
60mm inner diameter
gradient coil SPS 100
LITE version of
sample handling for
small lab settings
Table Top Sample
Handling System
Detail of Table Top Option
Cradle OD
Nominal Sample weight
RF coil Positioner confi guration Magnet bore size for 4.7T – 11.7T
mm Up to gmVolume/
phased arrayMillipede Brain Cardiac Surface LITE Table Cart
RF shielding Option
160mm 210mm 310mm 400mm
29 25 ● ● ● ● ● ● ●
33 25 ● ● ● ● ● ● ●
38/39 30 ● ● ● ● ● ● ● ●
62 30 ● ● ● ● ● ● ● ● ● ●
71 300 ● ● ● ● ● ● ● ● ● ● ● ●
138 300 ● ● ● ● ● ● ● ● ●
148 300 ● ● ● ● ● ● ● ● ●
Designed for Ease-of-use
Agilent’s sample handling products are able to meet customer
demands, whatever the specifi cations. Each is made to a very
high standard, and greatly improve ease of sample handling
and image quality.
We offer two options: the LITE system for unscreened rooms,
and either the table top or cart system for screened rooms. .
Features and Benefi ts
• Fine adjustment from the end of the positioner ensures
repeatable placement of your sample in the iso-centre.
• Positioners and sample cradles are available across our
entire range of gradients.
• Easy adjustment of the table height seamlessly accomo-
dates sample preparation and insertion.
• The large fl at surfaces of the table and cart allow conven-
ient access to additional equipment.
9
SoftwarePulse Sequence Library
FSEMS protocol
GEMS protocol
Agilent Pulse Sequence Library
The Agilent MRI sequence library is constantly being revised,
updated, and enhanced. The sequences are fully parameter-
ized for maximum fl exibility.
• VnmrJ 3.1 Software
• Standard 2D Imaging Sequences
• Diffusion-weighted 2D Imaging Sequences
• Standard 3D Sequences
• Advanced MRI Sequences
• Magnetic Resonance Spectroscopy (MRS) Sequences
• Shimming
Standard 2D Imaging Sequences
All standard 2D sequences described below are designed for
ease of use.
• SEMS – A 2D spin-echo MRI
• MEMS – A 2D multi-echo MRI
• GEMS – A 2D gradient-echo MRI
• MGEMS – A 2D gradient-echo MRI with multi-echo
acquisition
• GEMSIR – An inversion recovery MRI with 2D gradient-
echo - can be used for T1map
• FSEMS - A 2D Fast Spin Echo MRI
• FLAIR – Fluid-attenuated inversion recovery MRI
• Echo Planar 2D Imaging Sequence - Designed for use by a
non-MRI expert using a simple setup pre-scan for routine
EPI imaging
Advanced MRI Sequences
• Cardiac MRI – for looking at phases of the cardiac cycle
and reconstruction of the images for creating CINE views of
the beating heart
• Arterial spin labeling MRI – for measuring perfusion
• EPI-FAIR – slice-selective inversion recovery pulse on the
imaging slice. The control is a non-selective inversion
recovery pulse.
• EPI-STAR – slab-selective inversion recovery pulse applied
below the imaging slice. The control is above the imaging
slice.
• EPI-PICORE – slice-selective inversion recovery pulse
applied below the imaging slice. The control is a non-
selective inversion recovery pulse.
• SSFP – A steady-state free precession MRI
• Localized Spectroscopy - LASER, SPECIAL, Short Echo
STEAM
10
Site Planning
Pre-clinical 9.4T/160
imaging suite with
preparation area
Pre-clinical 7T/310
imaging suite with
separate preparation room
Your Partner in Planning
Evaluating and deciding on the right MRI system takes time,
but it is just the beginning of our relationship with you the
customer.
Here at Agilent, we know that our role doesn’t stop when
your system is ready. Site planning, and ensuring the correct
pre-requisites are in place, are just as important as helping
you select which system meets your research needs.
We will be on hand to help you plan and prepare the loca-
tion for the installation of your new imaging equipment. This
includes an additional site survey to identify and eliminate
potential issues that could impact operation of the magnet
once it is energized.
Maximum Capability, Minimum Footprint
All of our systems are designed to have the smallest
physical footprint possible, while providing you with
maximum imaging capability.
We recommend you have 3-4 rooms dedicated to your new
MRI system, but we will work with you to fi nd a solution to
whatever space constraints you may have.
Implementation Timeline
The implementation of our system is designed to be as effi -
cient as possible, allowing you to continue with your research
quickly.
The four key dates of the system implementation timeline are:
• Start of the project
• Build of the installation environment
• Delivery and installation of magnet system
• Commission and hand-over to the customer
Protecting Your Assets
RF shielding is an available option
with all of our systems, giving you
peace of mind that your installation
will provide a safe working environ-
ment for all personnel who come into
contact with the equipment.
The RF room is a custom, insulated,
turn-key magnet room, which includes
effective sound proofi ng designed to
shield the room’s exterior, while an
oxygen monitor gives you clear indica-
tion of the working conditions inside.
1111
Agilent is a name that is synonymous with measurement.
With expertise that spans electronic measurement, chemical
analysis and the life sciences, you can be sure that our prod-
ucts will meet your toughest requirements.
We pride ourselves on providing the world’s most complete,
most reliable laboratory productivity solutions, optimized for
your applications and workfl ows. Through a combination of
industry-leading instruments, accessible scientifi c expertise,
The Measure of Confi dence
Discovery MR901 (7T/310)
Agilent's magnet facility in Yarnton, UK.
9.4T/310mm magnet
easy-to-use software and a full range of global support
services, we are committed to delivering better results, faster
than ever.
Our MRI systems are no different. Our extensive expertise
dates back to the founding of Magnex Scientifi c in 1982. As
a result, our customers have benefi tted from the experience
and knowledge of our design teams and scientists, who bring
a wealth of information into the design of each and every
system we build.
www.agilent.com
Product specifi cations and descriptions in this document are subject to change without notice.
© Agilent Technologies, Inc., 2011Published in USA, July 01, 2011Publication Number 5990-8104EN
At a Glance
We have provided this table so that you can compare our
best selling systems at a glance. Further information can
be found inside this brochure, or by contacting us using
the information on this page.
For more information
Learn more:
www.agilent.com/chem
Find an Agilent customer center in your country:
www.agilent.com/chem/contactus
U.S. and Canada
1-800-227-9770
Europe
Asia Pacifi c
Contact the MRI team
Magnet System 7T (300MHz) 9.4T (400MHz)
Bore size 210 310 210 310
Length 1280mm 1636mm 1420mm 1719mm
Width 1200mm 1690mm 1690mm 1740mm
Min. ceiling height 3125mm 3030mm 3030mm 2990mm
Homogeneity (ppm/DSV)
Fully shimmed < ±2 / 8cm
< ±2.5 / 15cm
< ±2 / 8cm
< ±2.5 / 14cm
Superconducting only < ±4 / 8cm
< ±10 / 15cm
< ±4 / 8cm
< ±10 / 14cm
Fringe Field (5 Gauss line)
Radial 1.5m 2.1m 2m 2.2m
Axial 1.4m 2.6m 3m 3.6m
Main Gradient
Outside diameter 205mm 305mm 205mm 305mm
Inside diameter 120mm 210mm 120mm 210mm
Peak current 300A 300A 300A 300A
Max. gradient strength 600mT/m 300mT/m 600mT/m 300mT/m
Sample Positioning (optional)
Trolley
OD of optional positioners available
N/A 210mm N/A 210mm
120mm 120mm 120mm 120mm
60mm 60mm 60mm 60mm
Table
OD of optional positioners available
N/A 210mm N/A 210mm
120mm 120mm 120mm 120mm
60mm 60mm 60mm 60mm
LITE
OD of optional positioners available
N/A 210mm N/A 210mm
120mm 120mm 120mm 120mm
100mm 100mm 100mm 100mm
90mm 90mm 90mm 90mm
60mm* 60mm* 60mm* 60mm*
* No additional cradles are required for use with this positioner
Applications of RF Coils
SurfaceDual-Tuned
VolumePhased Array
Millipede
Oncology ✓ ✓ ✓
Spectroscopy ✓ ✓ ✓
Neurology (Brain and Spinal)
✓ ✓ ✓ ✓
Cardiac Scanning ✓ ✓
Micro-Imaging ✓ ✓
Whole Body Anatomic Scanning
✓ ✓
VisualSonics
Vevo2100 System& Vevo LAZR Image
System
SI healthcareJangwoo Cho
v1.0
<High frequency Preclinical Ultrasound System & Photoacoustic Image
System>
Functional/Physiological
Anatomical
Contrast
Image-Guided
Techniques
One Platform, Multiple Applications
High frequency Preclinical Ultrasound System
Superior resolution and image
uniformity throughout the Entire
field of view
30 micron resolution
Wide field of view
Medical grade monitor provides
higher resolution,
non-glare view from any angle
Photoacoustic Imaging Platform
Technical Specifications:
• Real-time, in vivo imaging of deep tissue
(up to 1 cm)
• Integrated, 20Hz tunable laser
(680-970 nm)
• Resolution down to 45 µm
• Imaging through endogenous
hemoglobin signal
• High optical contrast co-registered with
high-resolution imaging
Imaging station
Mouse & Rat Table
Temperature controlled platform maintaining
optimal physiological conditions for small animals
Integrated & displayed physiological monitoring –
ECG, heart rate, core temperature,
respiration
Transducer mounting system – for precision,
accuracy and hands – free scanning
3D positioning system
Accessory
Anesthesia System for Animal
Image Acquisition
Vevo MicroScan
MS700 : 70 MHz MicroScan transducer
- Broadband Frequency: 30 MHz - 70 MHz
- Applications: mouse embryology, epidermal imaging, superficial
tissue, subcutaneous tumors (< 9 mm), mouse vascular, ophthalmology
- Max Frame Rate: 476 fps
- Image Width: 9.7 mm, Image Depth: 12 mm
- Image Axial Resolution: 30 �m
Vevo LAZR
LZ250 : 13-24 MHz Integrated Fiberoptic Transducer
- Integrated fiberoptics for pulsed laser light delivery
- 256 sensitive piezoelectric elements for acoustic detection
- Broadband Frequency: 13 MHz - 24 MHz
- Image Width: 23 mm, Image Depth: 30 mm
- Image Axial Resolution: 75 �m
LZ550 : 32-55 MHz Integrated Fiberoptic Transducer
- Integrated fiberoptics for pulsed laser light delivery
- 256 sensitive piezoelectric elements for acoustic detection
- Broadband Frequency: 32 MHz - 55 MHz
- Image Width: 14.1 mm, Image Depth: 15 mm
- Image Axial Resolution: 40 �m
• LV Function
• Cardiac Output
• FractionalShortening
• VTI Interval
• Valvular Stenosis
• Vital Signs/BP integration
• RespiratoryGating/ECG
• Embryon lethal viability
• Great Vessels image and Dopplermeasurement
• Monitor Surgical sutures/hypertension
• Plaqueprogression
• Renal insuffiency
• Targetedcontrast to VCAM, P-Selectin
• Tumor size volumes
• Quantify relative vascular flow
• Monitor tumor metastasis
• TargetedMolecularImaging
• VEGFR2
• 3-D Modeling
• Injections
• Track Pharmaco-dynamic effects of novel compounds in real time
• Organ System Imaging
• Organ Blood flow at 30 microns
• Evaluate Path physiologiceffects of novel compounds
• Cardiac Function
• Renal Function
• Inflammation with molecular targets
• Injections and biopsy
• Tissue stiffness with DRF
• MonitorInflammation with molecular targets
• Injections
• Image joints
• QuantifyExpression with targetedcontrast from biotinilatedligand of choice
Ultrasound
Cardiovascular Oncology Metabolic Toxicology Immunology
Bridging Multiple Therapeutic Areas
Embryo through to adulthood
AdultEmbryo
Phenotypic Screening from Embryonic Stage to Adulthood
Video Loops
Overview of Research Areas
• Cardiovascular Research• Cancer Research• Abdominal & Reproductive Imaging• Developmental Biology• Photoacoustic Imaging
Cardiovascular Research
Parasternal Long Axis
Video Loop
Parasternal Short Axis
Video Loops
Carotid Artery
Video Loops
10 w ApoE/LDLr DKO mouse CCA
80 w ApoE/LDLr DKO mouse CCA
1 mm
40 w ApoE/LDLr DKO mouse CCA
Image sequence courtesy of Gan et al, University of Gothenburg, Sweden
Plaque Build-up in Carotid Arteries in Adult Mouse
Cancer Research
• Screen for pre-palpable tumors• Quantification in 2D and 3D• Quantification in vascularity using
� Color Doppler� Power Doppler� Contrast Agents (MicroMarker)
• Quantification of Biomarkers (VEGFR2, Integrins…)
Cancer ImagingOrthotopic Tumor – B-Mode Imaging
*Images and histology courtesy of Dr. Marris & Nick Rhodin, Children's Hospital of Philadelphia
Pre-PalpableTumor (60 mm3)
Earliest PalpableTumor (180 mm3)
Cancer ImagingSubcutaneous Tumor – 2D B-Mode Imaging
Cancer ImagingSubcutaneous Tumor – 3D B-Mode Imaging
Volume = 115.95mm2
Video Loop
Vevo MicroMarker Contrast Agents
Exclusive Partnership:Bracco Group
Joint development and manufacturing of MicroMarker™ kits
Video Loop
Abdominal & Reproductive Imaging
• Midline Abdominal Landmarks • Adrenal Glands• Kidney• Pancreas• Ovary
Midline Abdominal Landmarks
Video Loop
Adrenal Gland
Video Loops
Mouse Left Kidney
Video Loop
Developmental Biology
1 mmAmnioticCavity
CoelomicCavity
EctoplacentalCavity
Atlas of Mouse Development, MH KaufmanFoster FS et al., Ultrasound in Med and Biol, 28:1165-1172, 2002
Embryonic Mouse Development at E7.5
Sagittal Profile at E12
Video Loop
Color Doppler in Placenta at E12
Video Loop
Injection into brain at E12
Video Loop
Profile at E17
Video Loop
Color Doppler demonstrating blood flow through the umbilical cord
Video Loop
Color Doppler – Umbilical Cord
Photoacoustic Imaging
Pulsed Laser Illumination
OpticalAbsorption
Heating of Light Absorbing Volume
ThermalExpansion
Pressure Waves
AcousticDetection
Optical Path Sound Path
Impactful images with the sensitivity of optical imaging and the resolution of
ultrasound
Novel features of Vevo LAZR system
• Inherent co-registration of photoacoustic and anatomical images� Simultaneous registration of photoacoustic image on 2D and 3D planes� Real-time acquisitions for true in vivo monitoring
What is this signal?
Where is this signal?
Needle SkinTumorNeedle SkinTumor
Delivery of nanoparticlesinto tumor
Microvascular Imaging –Mouse skull, superficial cortex
Inherently co-registered high-resolution anatomical image and high-sensitivity photoacoustic signal acquired transcranially in vivo from the mouse superficial cortex (skull and skin intact, hair removed)
Sensitive photoacoustic signal acquired transcranially in vivo from the mouse superficial cortex (skull and skin intact, hair removed). Projection of 3D data, C-Scan shown.
Hindlimb ischemia
3D images of mouse hindlimb during
ischemia Unilateral hindimb ischemia: model of peripheral arterial disease (PAD)-low mortality rate-ease of access to femoral artery
3080 yonge street suite 6100box 66 toronto canada M4N 3N1
US
100 park avenue, suite 1600new york, NY 10017
Europe
Science Park 4061098 SM AMSTERDAMThe netherlands
www.visualsonics.com
+1 416 484-5000
TOLL FREE >
1.866.416.4636 (north america)
+ 800 0751.2020 (europe)
Advancing preclinical research
CardiovascularCancerDevelopmental BiologyDiabetesNeurobiologyReproductive BiologyRegenerative MedicineOphthalmologyMolecular ImagingOrthopedicGene Delivery
Thank You
VISUAL SONICS Korea: SI Healthcare – Mr. Jangwoo Cho
실험동물용 고해상도 디지털 엑스선 CT 영상 솔루션
NanoFocusRay Co., LTD.
June 2011
NFR Polaris-G90
전임상소동물용 in-vivo마이크로 CT
SPECIFICATIONS
X-ray sourceSealed type microfocus tube20 ~ 90kvP, 180uA, Max. 8Watts
X-ray detectorFlat Panel Detector 145x115 mm, 74.8 um Pixel size
Scanning time < 15sec for 360views
Magnification 1.4x ~ 10x, variable zooming mechanism
FOV Transaxial ~100mm, length ~200mm120mm large bore size
Rotation method 360°Continuous rotation using by Slip-Ring
Resolution < 13um voxel size
ReconstructionVolumetric Cone-beam Reconstruction (FDK)In-line / Off line mode
Radiation safety< 1uSv/h at any point on the instrument surface
Size / Weight 1250(W)x1450(D)x1600(H) mm, 350kg
Precision Gantry Rotation SystemHigh speed cone-beam reconstruction
X-ray 튜브Micro-focus X-ray tube
X-ray 검출기Flat Panel Sensor
샘플 스캔 메카니즘
Gantry rotation
배율가변 메카니즘
Slip-ring technology
호흡마취 시스템
호흡 및 심장 게이팅 시스템 (Optional)
제어시스템
System control and image acquisition
영상재구성 모듈
Reconstruction, 3-D viewer and image analysis
CabinetRadiation shield cabinet (1uSv/h)
NFR Polaris-G90 장비 구성
샘플 스캔 메커니즘
Variable zooming mechanism 적용
가변 배율 : 10x ~ 1.4x
FOV : transaxial 100mm ~ 14mm
직경 10mm 이내의 적출 시료
~ 직경 100mm 크기의 소동물까지 촬영 가능함.
Field of views (FOV) : Full body scan
Up to length 200mm
배율에 따른 FOV와 Voxel size ( 1 scan 수행 시)
배율FOV (mm)
[transaxial x length]Voxel Size (um)
[X x Y x Z]
1.4x 100.2 x 82.1 101.4x80.1
2.0x 70.1 x 57.4 71.0x56.1
4.1x 34.2 x 28.0 34.6x27.4
10.0x 14.0 x 11.5 14.2 x 11.2
Physiological Monitoring & Gating
Small animal gas anesthesia systemQuickly anesthesia for rats and mice use with isofluraneEase of anesthetic control
Gas anesthesia Mask ; keep an inactive of sleeping stateVisual monitoring using by CCD camera with IR LEDs
Allows the operator to see a real-time image of the animal during scanningThe gating software analyzes the 30frames/sec video stream and provide a respiratory gating signal for synchronized scanning.Method of generating respiration gating signals for x-ray micro computed tomography scanner (Korean patent application 10-2010-0004635)
Carbon WireElectrode
CCD camera
Video motiondetection
Respiratory & Cardiac Gating
NFR Polaris-G90 제품 구성
제품 구성품
X-ray Micro-CT
소동물용 호흡마취 마스크 1set
소동물용 베드 1set
소동물용 마취 시스템 / 예비마취챔버 1set
호흡 게이팅 시스템 1set (Optional)
사용자 매뉴얼(CD 포함)
설치 사양
정격전압 - AC 220V/60Hz 10A, 접지, 별도의 단자함 설치
외형치수 - 1300(W)x1420(D)x1700(H)mm
제품무게 - 350kg
Kwon-Ha Yoon, et al., “Articular Cartilage Imaging by the Use of Phase-contrast Tomography in a Collagen-Induced Arthritis Mouse Model”, Academic Radiology (2010)
Kwak Han Bok et al., “Inhibition of osteoclast differentiation and bone resorption by Rotenone, through down-regulation of RANKL-induced c-Fos and NFATc1 expression”, Bone (2009).
Jaemin Oh et al., “Risedronate Directly Inhibits Osteoclast Differentiation and Inflammatory Bone Loss”, Biol. Pharm. Bull. (2009).
Kang Y, Choi M, Lee J, Koh GY Kwon K, et al., “Quantitative Analysis of Peripheral Tissue Perfusion Using Spatiotemporal Molecular Dynamics”, PloS ONE (2009).
Hye Won Kim, Kwon-Ha Yoon, et al., “Micro-CT Imaging with a Hepatocyte-selective Contrast Agent for Detecting Liver Metastasis in Living Mice”, Academic Radiology (2008).
Chul Ho Jang, et al., “Mastoid obliteration using a hyaluronic acid gel to deliver a mesenchymal stem cells-loaded demineralized bone matrix: An experimental study”, International Journal of Pediatric Otorhinolaryngology (2008).
Quan-Yu Cai, Kwon-Ha Yoon, et al., “Colloidal Gold Nanoparticles as a Blood-Pool Contrast Agent for X-ray Computed Tomography in Mice”, Investigative Radiology (2007)
Lucia Martiniova, Karel Pacak, et al., “In vivo micro-CT imaging of liver lesions in small animal models” Methods (2009)
NFR micro-CT를 이용한 연구
주 요 고 객
설치예정
Image Gallery
32
Dental Application
Spine Image
Spine Bone fusion in Rat
35
Anatomical Record,2002
Mouse Inner Ear by micro CT
BA-1 A-2
3D Volume Rendering & CPR
A. Volume of left atrium (LA), B. Volume of left ventricle (LV), C. Volume of right atrium (RA), D. Volume of right ventricle (RV)
A B C D
LA LV RA RV
Vol.(cm3) 0.747 0.966 0.675 0.763
3D Volume Measurement of each cardiac chamber
BA
Plaque Analysis
Aortic Aneurysm Model of Mice
주식회사나노포커스레이
전북전주시덕진구팔복동 2가전북테크노파크테크노빌 B-114Tel. (063) 262-6795, Fax. (063) 262-6796
김경우(010-7504-8811, [email protected] )www.nanofocusray.com
Thank You!
philips
BV-Pulsera
I. System overview
philips
BV-Pulsera
1. Battery management
: 만약 energy level 이 특정한 %아래로 떨어지면, C-arm stand 에
경고표시가 나타난다. . C-arm stand 와 mobile view station 둘 다
연결되어 있어야만 하며 mobile view station 은 main power outlet
socket 에 연결되어 있어야만 한다.
이것은 재충전을 하기 위해서이며 C-arm stand 의 왼쪽 핸들 쪽에
connector panel 에서 오렌지색 불빛이 나타난다..
2. System ON/OFF
1) Switching the system on
C-arm stand 이나 mobile view station 위의
system on key 를 누른다.
2) Switching the system off
mobile view station 위의 system off 를 누른다.
philips
BV-Pulsera
단, c-arm 의의 c-arm stand off 만 누르면 오직 c-arm
stand 만 꺼진다.
3) Emergency Power Off
: 응급상황이 발생하였을 경우, 모든 작동을 멈추게 한다.
하지만, mobile view stand 의 main power plug
를 제거 해 주어야 한다.
3. Managing patients and examination
: 환자와 검사는 ‘Administration’ screen 에서 조작 할 수 있다.
philips
BV-Pulsera
Schedule – 검사를 위해 예약된 환자 확인
Review – 시스템에 저장되어 있는 획득된 검사 확인
4. Adding a new examination
1) ‘Administration’ screen 에서 ‘schedule’버튼을 누른다.
2) ‘Add’ 버튼을 누른다.
3) keyboard 를 사용하여 ‘Patient name’영역에서 환자의 이름을
넣고 tab key 를 눌러서 다음 영역으로 이동한다.
4) ‘Patient ID’에 환자 ID 를 넣고 tab 을 눌러서 다음으로 이동한다.
5) ‘Date of Birth’에 환자의 생년월일을 넣는다.
6) ‘Sex’에 성별을 입력한다.
7) ‘Type’에서 메뉴를 내려서 검사 type 을 선택한다.
8) ‘Physician’에서 메뉴를 내려서 의사 이름을 선택한다.
9) 새로운 검사를 확인하기 위해 OK 버튼을 누른다.
5. modifying and Deleting an examination
: schedule list 와 review list 에서 검사는 수정할 수 있다.
list 에서 획득한 검사항목을 선택한 후 administration
screen 에서 modify 또는 delete 할 수 있다.
6. Selecting a patient for acquisition
philips
BV-Pulsera
1) schedule list 로부터 환자를 선택한다.
2)start examination 버튼을 누른다.
3)선택된 검사는 acquisition 검사가 된다.
4) Administration screen 는 검사 모니터 위에 black screen 에 의해
재배치 된다. 환자의 이름은 스크린의 가운데에 나타나고
acquisition 이 준비된다.
7. Standard DICOM export tasks
1) ‘Administration’스크린에서 ‘Export’를 클릭한다.
2) 원한다면 ‘Accession Number’ Field 에서 환자를 선택하기 위해
Accession number 를 넣을 수 있다.
3) ‘Target’ list 로부터 필요한 network 장치를 선택한다.
4) ‘Image Selection’ option 에서 Image 를 선택한다.
5) ‘Image format’ option 에서 필요한 format 을 선택한다.
6) 모든 setting 이 올바를 때 ‘ok’버튼을 클릭한다.
II. Image Essentials
philips
BV-Pulsera
1) Image Intensifier size 변경
또는 remote control 에서 변경한다.
2) Contrast and Brightness 조정
감소 증가
되돌리기
philips
BV-Pulsera
- contrast 와 brightness 기능을 자동으로 조절하기 위해
“Auto” 키를 누른다.
3) Rotate Image
얻고자 하는 곳까지 이미지를 회전하기 위해서 key 를 계속 누르고,
원래의 위치로 이미지를 복구 시키기 위해서 “0”점을 누른다.
4) Mirror Image
Image 를 좌우 또는 위 아래로 돌린다.
5) Iris and Shutter Adjustments
원하는 shutter 를 선택하여 누른다.
두 가지 shutter 모두를 조정하려면 두
개의 key 를 동시에 누른다.
philips
BV-Pulsera
선택된 shutter 회전, reset 은 “0”점을
누른다.
iris 를 안쪽 혹은 바깥쪽으로 이동,
reset 은 “0”점을 누른다.
III. X-ray control
philips
BV-Pulsera
1) Automatic kV/mA control
: 초기상태에 의해 이미지 밝기는 kV 와 mA 값을 바꿈으로써
자동으로 조절할 수 있다.
2) Manual kV/mA control
: 자동제어를 수동으로 바꿀 수 있다.
(kV 버튼을 누른다.)
3) Automatic mA increase in LDF mode
: 이것 또한 High penetration mode (HIP)라 부른다.
시스템이 가장 높은 kV 값에서 좋은 이미지를 만들어 낼 수 없을
때, 더 높은 mA 값에 의해 자동 조절 될 것이다..
(한계 시간은 30 초이다. 이 시간 후에 시스템은 normal mA 로
스위치가 돌로 돌려진다.)
IV. Making Image
philips
BV-Pulsera
1. Making a Low Dose Fluoroscopy (LDF) image
: LDF imaging 은 외과 수술 진행 동안 안내하고 C-arm 의 위치를
조정하는 목적을 위해서 추천한다.
1/4dose 와 1/2 dose LDF
1/4dose 또는 1/2 dose LDF 는 C-arm stand console 에 'X mode'의
soft key 를 사용해서 선택될 수 있다. 이러한 기술은 더 적은 이미지를
획득함으로써 dose 를 saving 하는 기술이다.
• 1/2 dose - 12.5 fr/s
• 1/4 dose - 6 fr/s
: LDF 이미지를 만들기 위해서, 왼쪽 hand switch key
또는 foot switch 의 왼쪽 pedal 을 눌러라.
(Storage speed 가 0 일 때, LIH 는 다음 LIH 에 의해 겹쳐질 것이고
disk 에 저장되지 않는다.)
image 는 Live monitor 에 나타난다. 다음 run 을 시작하면
image 는 다시 LIH 될 것 이다.
LDF 동안 다음 이미지가 보여지고 저장하기 위해서
remote control 또는 mobile view station 에서 Grab key 를 눌러라.
그럼 새로운 run 이 발생하여도 저장 되어 진다.
2. Making high quality images
philips
BV-Pulsera
: Hand 스위치의 오른쪽 key 또는 foot 스위치의 기능은 선택된
examination type 과 technique 에 의해 자동으로 결정된다.
*exposure technique
- digital exposure
- pulsed exposure
- radiography
*fluoroscopy technique
- high definition fluoroscopy
: Switching X-ray mode - X-ray mode 는 항상 바꾸는 것이 가능하다.
'X-mode' soft key 를 눌러서 원하는 mode 를 선택해라.
: Switching techniques - Fluoroscopy 와 Exposure technique 은 항상
바꾸는 것이 가능하다. 'Technique' soft-key 를 눌러서 바꾸어라.
: Storage - LIH 의 storage speed setting 에 관계없이 항상 disk 에
저장할 수 있다.
(1) Digital Exposure (SharpShot)
: Digital exposure (또한 SharpShot 이라고도 한다) 는
고화질로 저장하는데 사용된다.
1) 'X mode' key 를 눌러서 Digital exposure 를 선택해라.
2) single exposure 가 나오기 위해서 hand 스위치의 오른쪽 key
또는 foot 스위치의 오른쪽 pedal 을 눌러라.
philips
BV-Pulsera
kV control: digital exposure 동안 kV 와 mA 값은 고정되어 있다.
최적의 이미지 quality 를 확인하기 위해서 LDF 를 사용하여 scout
이미지를 만들고 적당한 kV 값을 정해라.
(2) Pulsed exposure
: Pulsed exposure 는 dynamic 이미지에 사용된다.
1) 'X-mode' soft key 를 누르고 'pulsed'를 선택해라.
2) 미리 조정된 pulse frequency 와 함께 pulsed exposure
sequence 를 시작하기 위해서 핸드스위치의 오른쪽 key 를
누르거나 foot 스위치의 오른쪽 pedal 을 눌러라.
pulse frequency : pulse frequency 를 바꾸기 위해서, 'Speed' key 를
눌러서 원하는 frequency 를 선택해라.
fr/s Max run time Max images in one
run
30 15s 450
15 30s 450
philips
BV-Pulsera
8 30s 240
5 30s 150
3 30s 90
(3) Pulsed fluoroscopy
: Pulsed fluoroscopy 는 dose saving mode 이거나 positioning
catheters 그리고 해부학상의 지역에서 빠르게 움직이는 장치를 위해서
움직임을 멈춰주는 mode 이다.
hand 스위치의 왼쪽 key 이나 foot 스위치의 왼쪽 pedal 을 눌러서
작동 되어진다.
(4) High Definition Fluoroscopy (X-ray mode continuous)
: Definition Fluoroscopy (HDF) 이미지는 저장하거나 고화질의 이미지를
만들 수 있다.
1/4dose and 1/2 dose HDF
1/4 dose 또는 1/2 dose HDF 는 C-arm stand console 에 'X mode'
soft key 를 사용하여 선택할 수 있다. 이 기술은 더 적은 이미지를
얻음으로써 dose 를 saving 하는 기술이다.
• 1/2 dose - 12.5 fr/s
• 1/4 dose - 6 fr/s
HDF 이미지를 만들기 위해서, 핸드스위치의 오른쪽 키 또는
foot switch 의 오른쪽 pedal 을 눌러라.
philips
BV-Pulsera
note: HDF acquisition run 은 30 초 후에 자동으로 끝난다.
3. Making vascular images
: Vascular image 를 얻기 위한 Examination type 을 선택한다.
그러면 'Mode' soft-key 는 사용 가능해지고 subtraction, trace,
roadmap, normal fluoroscopy 사이에서 선택이 가능해진다.
note
: vascular 이미지는 일반적인 fluoroscopy 가 선택된 이외의 mode 와
vascular 검사 type 일 때만 오직 가능하다.
(1) Performing subtraction
① 'Mode' soft key 를 누르고 나서 'Subtr'을 선택해라.
or 'Subtr' mode 를 고정시키기 위해서 foot 스위치의 중간 pedal
을 눌러라.
or 'Subtr' mode 를 고정시키기 위해서 remote control 에 |Mode|
key 을 눌러라.
mode 는 모니터의 오른쪽 아래 코너에 나타난다.
· storage frequency 를 바꾸기 위해서 'Speed'의 soft key 를 누르고
원하는 frame 수를 선택해라.
philips
BV-Pulsera
· HDF 는 subtraction 을 위해 더 좋은 이미지 quality 를 공급하기
때문에 LDF 보다 더 선호된다.
·subtraction 과정 동안 환자나 시스템을 움직이지 마라.
blurred 이미지가 발생할 수 있다.
② hand switch 오른쪽이나 foot switch 의 오른쪽 pedal 을
누른다.
③ mask 의 완료 후에 이미지는 회색으로 바뀌고 모니터에 'Inject'
메시지가 나타난다.
note
: subtraction 이 완전히 끝나기 전까지 hand/foot 스위치를 떼지 마라.
hand/foot 스위치가 해제될 때, 새로운 mask 가 fluoroscopy 가 다시
실행된 후에 즉시 만들어진다.
④ 조영제를 써서 injecting 을 시작해라. contrast bolus 의 이미지가
모니터에 나타날 것이다.
⑤ contrast bolus 가 사라지자 마자 Hand 또는 Foot 스위치를
해제해라
note
: 실행되는 각각의 이미지를 보기 위해서 mobile view station 에서
|Previous| 그리고 |Next| key 를 사용하거나 remote control 에서
아래의 버튼을 사용해라.
얻어진 이미지들을 확인하기 위해서 mobile view station 에서
|Run cycle| key 또는 remote control 에서 아래 버튼을 눌러라.
philips
BV-Pulsera
(2) Performing roadmap after subtraction
① 이전에 얻은 subtraction run 으로부터 roadmap 으로 사용하기 위한
이미지를 선택해라.
Or 'Mode' key 를 누르고 'Roadmap'을 선택해라.
Or 'Roadmap' mode 를 고정하기 위해서 foot 스위치의 중간 pedal 를
눌러라.
② 'Roadmap' mode 를 고정하기 위해서 remote control 에 |Mode| key
를 눌러라.
mode 는 모니터의 오른쪽 아래 코너 쪽에 나타난다.
③ 핸드스위치의 왼쪽 key 나 foot 스위치의 왼쪽 pedal 을 눌러라.
: 시스템은 roadmap 으로 스위치가 돌려진다. mask 이미지는 뒤집힐
것이고 혈관은 흰색으로 보여진다. 'Catheter' 메시지가 모니터에
나타난다. 이미지는 catheter 유도용으로 사용될 수 있다.
note
: 이전에 실행한 이미지를 사용할 때 BV Pulsera/patient 는
그사이에 움직여서는 안 된다.
'Roadmap' mode 에서 radiation 은 멈출 수 있고 mode 에서
나가지 않고 시작할 수 있다.
philips
BV-Pulsera
(3) Remasking
: 초기값에 의해 첫 번째 이미지 실행은 mask 로 사용된다. 그러나
mask 로써 같은 실행으로부터 다른 이미지를 사용하는 것이 가능하다.
① mobile view station 에서 |remask| key 를 눌러서
subtraction 을 꺼라.
② 새로운 mask 를 하기 위해 이미지를 선택해라.
③ subtraction 을 켜라.
: remote control 을 사용해서 remask 없이 subtraction 을 끄고 켤 수
있다. (새로운 mask 가 선택되어지지 않을 것이다.)
* SmartMask
: reference 모니터에서 이미지는 remote control 에 |SmartMask| key 를
눌러서 roadmap 으로서 사용될 수 있다.
Fluoro image 가 아닌 exposure image 를 roadmap image 로 사용할
수 있다.
(4) Bolus chase
① 'Technique' soft-key 를 눌러서 'Exposure'를 선택해라.
② 'X-mode' soft-key 를 눌러서 'Pulsed'를 선택해라.
③ bolus chase 를 위해서 초기 setting 값은 필요조건에 따라야 한다.
만약 아니라면, 'Speed' soft-key 를 누르고 올바른 pulse
frequency 를 선택해라.
philips
BV-Pulsera
④ 핸드스위치의 왼쪽 key 또는 foot 스위치의 왼쪽 pedal 을 누르고
bolus chase route 의 covering 을 확인하기 위해서 fluoroscopy 동안
지속적으로 C-arm 이나 tabletop 을 움직여라.
⑤ Hand or Foot 스위치를 떼고 bolus chase 의 시작위치로 C-arm 또는
tabletop 위치를 놓는다.
⑥ 핸드스위치 오른쪽 key 또는 foot 스위치 오른쪽 pedal 을 누르고 C-
arm 과 tabletop 을 지속적으로 움직이면서 inject 된 조영제를
따라간다.
⑦ bolus chase route 의 끝에서 exposure run 을 멈추기 위해 Hand or
Foot 스위치를 해제 한다.
Tip
- Leg coverage
: 12" II 는 같은 시간에 2 개의 leg 을 cover 하는 것이 가능하다. 9" II 는
오직 하나의 leg 만 cover 하는 것이 가능하다.
philips
BV-Pulsera
4. Standard DIOCM package
:examination image 를 저장하기 위해서 병원이나 부서의 network 에 보내게
해줍니다. 이 option 을 사용하기 위해서는 기본적으로 병원이나 부서의
network 에 반드시 연결되어 있어야만 합니다.
1. ‘Administraion’ 스크린에서 ‘Export’를 클릭합니다.
2. ‘Export’ panel 이 나타납니다.
만약, 필요하다면 Auto matching 을 위해 ‘Accession Number’ field 에
Accession number 를 넣을 수 있습니다. 단, Name 과 Patient ID 는
조정할 수 없습니다.
3. ‘Target’ list 로부터 필요한 network 장치를 선택합니다.
4. ‘Image Selection’ 에서 원하는 Image 를 선택합니다.
(각각에서 image 수가 쓰여져 있습니다.)
5. ‘Image format’ 에서 필요한 format 을 선택합니다.
(기본적으로 XA 로 넘기시면 됩니다.)
6. 모든 셋팅이 올바르면 ‘OK’버튼을 누르시면 됩니다.
