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Living Organisms Consist of Cells
•State the resolution and magnification that can be achieved by a light microscope•Explain the difference between magnification and resolution•Explain the need for staining samples in light microscopy
Resolution and Magnification
Car Headlights from far away Car Headlights Close-Up
Define the Following Terms
• Magnification• Resolution
Light Microscope
• Number of lenses used to produce an image
• Light passes from the bulb (or mirror) under the stage through a condenser lens and then through the specimen
• The light beam is focused through the objective lens then the eyepiece lens
• How do you calculate the magnification power of a light microscope?• Eyepiece lens magnification x Objective lens magnification
• The max magnification of a light microscope is x1500
• The max resolution of a light microscope is 200nm
An Electron Microscope
Chloroplasts Electron micrograph
Leaf cells in moss ; viewed using light Microscope; chloroplasts visible, but not detailed
Optical microscope image of blood cell smear.
SEM image of red blood cells.
TEM image of a thin section of a blood vessel. A white blood cell is entering the tissue between two epithelial cells.