Holistic active for dazzling skin

LitchidermTM

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IntroductionConcept of holistic active

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Skin reflects our state of mind, our health, our internal balance…

Everyday skin has to face to different stresses: a bout of tiredness and the skin immediately becomes dull, irritated and wrinkled.

Our concept

Overall protection via:

- performing antioxidant system- performing barrier function

…all of it provided by the delicate delight of a fresh fruit.

Skin is indulged and shows it off…

Holistic active

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ROS (H2O2, O21)

PGE2MDAMMP-1

Dull skinIrritated skin

Alteration of skin barrier function

Skin cells

Skin properties

Oxidative stress

Photo-agingUV stress

Holistic active

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ROS (H2O2, O21)

MMP-1PGE2MDA

Good skin moisturizationSkin complexion

Well-being

Skin cells

Skin properties

Protection against oxidative stress

Protection against UV stress

Protection against photo-aging

Holistic active

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Holistic active

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And what if all these properties could be offered by a fruit, a source of pleasure and vitality?

Holistic active

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The ingredient Origin and main components

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• Tree native to Southern China,where it has been cultivated forover 4000 years.

• Magnificent tree which can reach6-12 m high with evergreenleaves.

• The fruit has a red brittle shell andthe single black seed is covered bya white translucent flesh.

Lychee, tree

Litchi chinensis Sonn.

Lychee: botanical data

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• According to the poet Su Tung Po,lychee provides “happiness forliving”.

• Lychee can be eaten fresh, theflesh is fragrant, refreshing with asweet and sensual taste.

• Decoctions of roots, bark andflowers are traditionally used tocure throat ache.

• In China, the tea of the pericarp isdrunk to cure skin rashes.

Lychee, fruit

Lychee – traditional use

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LITCHIDERMTM is extracted from the pericarp of lychee, Litchi chinensis Sonn. which belongs to the Sapindaceae family.

"Spring is with Mt. Luofo all year round,Loquat and snowberries can consecutively be found. Eating three hundred lychees a day, I'd rather choose permanent stay“Su Tung Po

Litchiderm™

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HPLC-PDA analysis

Main components : catechuic tannins

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The overall protective activity of LITCHIDERMTM has been demonstrated by selecting different tracers of UV and oxidative stress.

Morever, good moisturization is an important part of skin protection, as only well hydrated layers can properly perform their barrier function.

Acting at the source on complementary levels allows to maintain a healthy and radiant skin.

Litchiderm™

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ROS (H2O2, O21)

MMP-1PGE2MDA

Good skin moisturizationSkin complexion

Well-being

Skin cells

Skin properties

Protection against oxidative stress

Protection against UV stress

Protection against photo-aging

LITCHIDERM™

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Litchiderm™ - efficacy summary

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Litchiderm™Efficacy tests: protection against oxidative stress

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Anti-free radicals (in tubo)

Protection against oxidative stress

Protocol

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LITCHIDERMTM has a protective activity against free radicals and reactive oxygen species.

Anti-free radicals (in tubo)

Protection against oxidative stress

Results

The scavenging effect of Litchiderm™ has been evaluated by chemical and biochemical enzymatic tests

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Anti-free radicals (in vivo)

Protocol

Protection against oxidative stress

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Anti-free radicals (in vivo)

Results

Protection against oxidative stress

In vivo study performed on 13 volunteers. Measurement of chemiluminescence level, at 0 and 10 minutes after treatment.

Litchiderm™ confirmed its anti-free radicals activity (in vivo) with similar results to the benchmark, tocopheryl acetate

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Litchiderm™Efficacy tests: protection against UV stress

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Cytophotoprotection on human keratinocytes irradiated by UVB (in vitro)

Protection against UV stressProtocol

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LITCHIDERMTM has showna potential to reducethe inflammatory reactionof UVB irradiated keratinocytes.

Cytophotoprotection on human keratinocytes irradiated by UVB (in vitro)

Protection against UV stressResults

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Measurement of released MDA and cellular proteins

Positive reference: vitamin E at 0.0003%

Cytophotoprotection on human fibroblasts irradiated by UVA (in vitro)

UVA

D0 Seeding of human fibroblasts in growth medium

D3 Replacement of growth medium by a medium containing LITCHIDERM™

Replacement of medium by a salt solutionD5 UVA

Irradiation (20 J/cm²)

Protection against UV stressProtocol

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LITCHIDERMTM has shown a good potential to protect human fibroblasts against UVA.

No toxic effect of tested products on cellular viability (protein level).

Cytophotoprotection on human fibroblasts irradiated by UVA (in vitro)

Protection against UV stressResults

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MMP - Background

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Synthesis

TIMP1:1 inactivation

Equilibrated degradation

LOW RENEWAL OF ECM

MACROMOLECULES

DERMAL HOMEOSTASIS MMPs

Fibroblast Collagen

MMP Background: role in normal skin

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Synthesis

TIMP

MMPs

Fibroblast Collagen

ECM degradation

Dermal homeostasis

MMP background: role in skin aging phenomenon

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Synthesis

TIMP

MMPs

Fibroblast Collagen

ECM degradation

Dermal homeostasis

UVA

ROS

MMP background: role in skin photo-aging phenomenon

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Laboratory negative of image analysis – Internal R&D

MMP-1

Control 0.75 MED 1 MED 2 MED0

0.5

1

1.5

2

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10

20

30

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Stained surface

Number of stained cells

n = 8 subjects

Control

1 MED

0.75 MED

2 MED

MMP-1 background: induction by UVR–Immunohistochemistry

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Litchiderm™Efficacy tests: protection against photo aging

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Evaluation of released MMP-1

Positive reference: dexamethasone at 0.04 ppm

D0 Seeding of human fibroblasts in growth medium

D1 Replacement of growth medium by a medium containing Litchiderm™

UVA

D3 Replacement of medium by a salt solution – Irradiation of fibroblasts by UVA: 15 J/cm²

Protection against photo-aging

Protocol

Inhibition of MMP-1 expression on human dermal fibroblasts irradiated by UVA (in vitro)

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LITCHIDERMTM has significantly reduced the expression of MMP-1 in UVA irradiated human dermal fibroblasts.

Protection against photo-aging

Results

Inhibition of MMP-1 expression on human dermal fibroblasts irradiated by UVA (in vitro)

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Preparation of human skin explantsin culture on EMEM + FCS at 1%

Incubation for 2 days at 37°CChange of the medium (Hanks) and UVB (0 and 300 mJ/cm²)

Change of the medium:EMEM + FCS at 1% + LITCHIDERM LS 9704

Incubation for 3 days at 37°C

Viability test (MTT) and measure of MMP-1 release

Protection against photo-aging

Inhibition of MMP-1 expression on skin explants irradiated by UVB (ex vivo)Protocol

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LITCHIDERMTM has significantly reduced the level of released MMP-1 in human skin explants irradiated by UVB without change in cell viability.

Protection against photo-aging

Inhibition of MMP-1 expression on skin explants irradiated by UVB (ex vivo)Results

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Litchiderm™Efficacy tests: moisturizing activity

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Moisturizing effect in vitro

Protocol

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Moisturizing effect in vitro

Results

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Litchiderm™Clinical results: improvement of skin complexion

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Improvement of skin complexion

Protocol

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Improvement of skin complexion

Results

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Conclusion

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By fighting against daily stress (oxidative stress - UV stress) and skin aging, LITCHIDERMTM also fights against the visible aspect of « depressed » skin.

LITCHIDERMTM offers an holistic approach of well-being:

- overall activity combined with the delicate delight of a fresh fruit.

Skin is indulged and shows it off…

Cosmetic benefits

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• “Fruity” and “delicacy” formulations to brighten and rejuvenate skin complexion

• Anti-aging “gourmet” skin care products

• Sensorial formulations

• Skin care for “cosmeto-food” concepts

LITCHIDERMTM is sold under license agreement with the University of Michigan USA covering US 6130254 and US 6365630 and their foreign counterparts.

Cosmetic applications

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Technical data

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“Although all statements and information in this publication are believed to be accurate and reliable, they are presented gratis and for guidance only, and risks and liability for results obtained by use of the products or application of the suggestions described are assumed by the user. SELLER MAKES NO WARRANTY OF ANY KIND, EITHER EXPRESS OR IMPLIED, BY FACT OR LAW, INCLUDING WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Statements or suggestions concerning possible use of the products are made without representation or warranty that any such use is free of patent infringement and are not recommendations to infringe any patent. The user should not assume that toxicity data and safety measures are indicated or that other measures may not be required. The claims and supporting data provided in this publication have not been evaluated for compliance with any jurisdiction’s regulatory requirements and the results reported may not be generally true under other conditions or in other matrices. Users must evaluate what claims and information are appropriate and comply with a jurisdiction’s regulatory requirements. Recipient of this publication agrees to (i) indemnify and hold harmless each entity of the BASF organization for any and all regulatory action arising from recipient’s use of any claims or information in this publication, including, but not limited to, use in advertising and finished product label claims, and (ii) not present this publication as evidence of finished product claim substantiation to any regulatory authority.”

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