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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
A TASTE FOR TROUBLE
A Case Study of Nutritional Health as an introduction to Macromolecules for High
School Biology Classes
This case study was written as a novel way to approach the study of macromolecules and the
role they play in life. Understanding the basic biochemistry of carbohydrates, lipids, proteins
and nucleic acids helps students grasp what cells are composed of and how they carry out life
processes. Most students find it easier to learn about something if they can connect it to their
daily lives. Each scene leads the class deeper into the study of macromolecules beginning with
Katrina’s health problems which stem from her food and activity choices which have lead to
Type II Diabetes, and beyond into exploring the changes in global nutritional health issues.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Table of ContentsA TASTE FOR TROUBLE..........................................................................................................1
A Taste for Trouble Case Study Lesson Plan Overview...................................................................3
Student Objectives:........................................................................................................................5
Timeline:.........................................................................................................................................6
Sequence:.......................................................................................................................................6
National Standards:........................................................................................................................7
State Standards:.............................................................................................................................8
Background Information...............................................................................................................16
Teaching Resources......................................................................................................................19
Activity Sheets for A Taste for Trouble Part I........................................................................23
A Taste for Trouble: Part II............................................................................................................25
Activity Sheets: A Taste for Trouble Part II...........................................................................27
A Taste for Trouble Part III............................................................................................................34
Activity Sheets: A Taste of Trouble Part III:..........................................................................38
A Taste for Trouble Part IV............................................................................................................39
Activity Sheets for A Taste for Trouble Part IV......................................................................41
A Taste for Trouble Part V:...........................................................................................................52
A Taste for Trouble Culminating Project...............................................................................54
References....................................................................................................................................55
A Taste for Trouble Answer Sheets...............................................................................................57
A Taste for Trouble Student Manual.............................................................................................68
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
A Taste for Trouble Case Study Lesson Plan OverviewThis case study was written as a novel way to approach the study of macromolecules
and the role they play in life. Understanding the basic biochemistry of carbohydrates, lipids,
proteins and nucleic acids helps students grasp what cells are composed of and how they carry
out life processes. Most students find it easier to learn about something if they can connect it
to their daily lives. Each scene leads the class deeper into the study of macromolecules
beginning with Katrina’s health problems stemming from her food and activity choices leading
to Type II Diabetes and beyond in exploring the changes in global nutritional health issues.
In A Taste for Trouble Part I, two students are introduced; Katrina a sophomore new to
the small town, who laments moving from her big city full of fast food choices and shopping
malls. She is overweight, hungry, thirsty and needs to urinate frequently; all classic signs of Type
II diabetes. She befriends Jeri, a skinny blonde so hungry she is about to pass out who is revived
by the gooey candy bar proffered out of Kate’s immense handbag. While not as detailed, Jeri’s
symptoms are classic for hypoglycemia.
In A Taste for Trouble Part II, we learn of Katrina’s college-aged cousin, Nicole, who is
determined to improve Katrina’s health with a diet and exercise program. Nicole gives Katrina
“homework” to do to calculate her calorie needs and start a food diary.
In A taste for Trouble Part III, Katrina has been trying to follow Nicole’s plan for
improvement and the girls have been meeting three times a week to walk around the park.
Katrina gets a blister on their first walk which has now become infected. She goes to the doctor
who orders blood tests and a urinalysis. Results of the tests lead students to a diagnosis of
diabetes.
The next segment of A Taste for Trouble Part IV brings Katrina to a series of lessons
with Sharon, a Diabetes Consultant at the local hospital. Sharon explains to Katrina that all food
is not equally nutritious. She gives Katrina a series of assignments to complete to help Katrina
understand why she will be asked to change her lifestyle.
The conclusion of A Taste for Trouble Part V brings Jeri back into the story. She is
feeling faint before class again and asks Katrina for a candy bar. Katrina hands her friend a
banana from her bag and tells her she thinks Jeri might have a condition which can result in
diabetes too even though she is skinny. They decide to focus their big project assignment on
finding out if there are more diseases related to diet. Their teacher encourages them to go
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
global and look at how global economy changes are affecting the prevalence of diabetes across
the world.
Each story segment is followed by activities and labs including teacher instructions for
lab set up and answer sheets. PowerPoint, video and internet sites are included where
indicated. A student manual includes all student worksheets, and lab instructions. Some labs
and activities may not fit into every teacher’s schedule or classes time frame but all contribute
to learning about macromolecules especially carbohydrates, nutrition and the role it plays in
health. This scheduling is based upon 45 minute class periods meeting each day.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Student Objectives:
1. Students will be introduced to the biochemistry of macromolecules; specifically carbohydrates; their structures and functions using a case study about a teen who suffers from Type II Diabetes.
2. Students will engage in activities and labs designed to introduce basic concepts about macromolecules such as dehydration synthesis and hydrolysis, polymers, calories, macro and micronutrients, Basal Metabolic Rate (BMR) and Body Mass Index (BMI), portion size, nutritionally based diseases and global changes in nutrition and disease.
3. Students will understand the detrimental effects of fats on the body and their relationship to heart disease, diabetes, and obesity
4. Students will identify pertinent information from a situation (the story) and use internet research to construct a list of potential food related health problems for teens.
5. Students will propose questions and medical laboratory tests designed to narrow a list of potential disease processes using information from their internet research.
6. Students will read tables, analyze results, and draw conclusions based upon data presented.
7. Students will learn to use simple assay techniques to determine the presence of certain macromolecules by building a rubric for organic molecule testing reagents.
8. Students will demonstrate understanding of how to read a food label by doing a comparison study of three nutrition labels.
9. Students will practice dehydration synthesis and hydrolysis with hands-on simulations.
10. Students will analyze their nutritional requirements and eating habits by keeping a food diary and learning to read nutrition labels.
11. Students will learn about America's growing obesity issues and how portion size can contribute to this problem.
12. Students will analyze the contents of a fast food meal for macromolecules after watching the video “Super Size Me”.
13. Students will analyze their reactions after viewing “Super Size Me!” by writing an essay.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
14. Student will communicate in a format of their choosing, their understanding of the global impact of changing dietary practices and increase of nutritionally based diseases.
Timeline: This case study is designed to be used in a 2 to 3 week time frame depending upon whether or not all of the labs are used and how accessible the internet is to student. Time could be shortened if assignments are given as homework rather than done during class time or lengthened to add more content.
Sequence: This case study should be used after a unit of basic chemistry and before studying cells. It is a desirable to have covered types of bonds, properties of water, pH, carbon bonding and functional groups. This case study introduces macromolecules and nutrition focusing on carbohydrates and through laboratories incorporates lipids and proteins to some degree. A deeper study of lipids and proteins would follow this unit prior to studying cells.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
National Standards:
Health
Standard 6: Understands essential concepts about nutrition and diet Standard 7 : Knows how to maintain and promote personal health
Life Science
Standard 9 : Understands the sources and properties of energy Standard 11 : Understands the nature of scientific knowledge
Standard 12 : Understands the nature of scientific inquiry
Standard 13 : Understands the scientific enterprise
Challenge students to accept and share responsibility for their own learning
Orchestrate discourse among students about scientific ideas
Behavioral biology has implications for humans, as it provides links to psychology, sociology, and anthropology.
The chemical bonds of food molecules contain energy. Energy is released when the bonds of food molecules are broken and new compounds with lower energy bonds are formed. Cells usually store their energy temporarily in phosphate bonds of a small high-energy compound called ATP.
The energy for life primarily derives from the sun. Plants capture energy by absorbing light and using it to form strong (covalent) chemical bonds between the atoms of carbon-containing (organic) molecules. These molecules can be used to assemble larger molecules with biological activity (including proteins, DNA, sugars, and fats). In addition, the energy stored in bonds between the atoms (chemical energy) can be used as sources of energy for life processes.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
State Standards:SCIENCE PROCESSES AND INQUIRY
Process Standard 1. Observe and Measure - Observing is the first action taken by the learner to acquire new information about an organism or event. Opportunities for observation are developed through the use of a variety of scientific tools. Measurement allows observations to be quantified. The student will accomplish these objectives to meet this process standard. 1. Identify qualitative and quantitative changes in cells, organisms, populations, and ecosystems given conditions (e.g., temperature, mass, volume, time, position, length, quantity) before, during, and after an event. 2. Use appropriate tools (e.g., microscope, pipette, metric ruler, graduated cylinder, thermometer, balances, stopwatches) when measuring cells, organisms, populations, and ecosystems. 3. Use appropriate System International (SI) units (i.e., grams, meters, liters, degrees Celsius, and seconds); and SI prefixes (i.e., micro-, milli-, centi-, and kilo-) when measuring cells, organisms, populations, and ecosystems.
Process Standard 3: Experiment - Experimenting is a method of discovering information. It requires making observations and measurements to test ideas. The student will accomplish these objectives to meet this process standard. 1. Evaluate the design of a biology laboratory investigation. 2. Identify the independent variables, dependent variables, and controls in an experiment. 3. Use mathematics to show relationships within a given set of observations (e.g., population studies, biomass, probability).
4. Identify a hypothesis for a given problem in biology investigations.
5. Recognize potential hazards and practice safety procedures in all biology activities.
Process Standard 4: Interpret and Communicate - Interpreting is the process of recognizing patterns in collected data by making inferences, predictions, or conclusions. Communicating is the process of describing, recording, and reporting experimental procedures and results to others. Communication may be oral, written, or mathematical and includes organizing ideas, using appropriate vocabulary, graphs, other visual representations, and mathematical equations. The student will accomplish these objectives to meet this process standard. 1. Select appropriate predictions based on previously observed patterns of evidence. 2. Report data in an appropriate manner. 3. Interpret data tables, line, bar, trend, and/or circle graphs. 4. Accept or reject hypotheses when given results of a biological investigation. 5. Evaluate experimental data to draw the most logical conclusion. *6. Prepare a written report describing the sequence, results, and interpretation of a biological investigation or event. 7. Communicate or defend scientific thinking that results in conclusions.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Process Standard 6. Inquiry - Inquiry can be defined as the skills necessary to carry out the process of scientific or systemic thinking. In order for inquiry to occur, students must have the opportunity to ask a question, formulate a procedure, and observe phenomena. The student will accomplish these objectives to meet this process standard.
3. Use a variety of technologies, such as hand tools, microscopes, measuring instruments, and computers to collect, analyze, and display data. 4. Inquiries should lead to the formulation of explanations or models (physical, conceptual, and mathematical). In answering questions, students should engage in discussions (based on scientific knowledge, the use of logic, and evidence from the investigation) and arguments that encourage the revision of their explanations, leading to further inquiry.
Standard 5: Matter, Energy, and Organization in Living Systems - Living systems require a continuous input of energy to maintain their chemical and physical organizations. The student will engage in investigations that integrate the process standards and lead to the discovery of the following objectives: 1. The complexity and organization of organisms accommodates the need for obtaining, transforming, transporting, releasing, and eliminating the matter and energy used to sustain the organism (i.e., photosynthesis and cellular respiration). 2. As matter and energy flow through different levels of organization of living systems and between living systems and the physical environment, chemical elements are recombined in different ways by different structures. Matter and energy are conserved in each change (i.e., water cycle, carbon cycle, nitrogen cycle, food webs, and energy pyramids).
Standard 6: The Behavior of Organisms - Organisms have behavioral responses to internal changes and to external stimuli.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Step by Step Procedure for Carrying out the Case Study
Session I:
Time allowed: At least one class period
Before you begin A Taste for Trouble Case Study, show your students a nutrition label (there is one on the Case Study PowerPoint) or pass a nutrition label around the room, and give your students an assignment to bring in 6 different nutrition labels from a variety of foods. Making this assignment now will make Session II go much smoother. It is also a good idea to collect additional food labels to use in Session II. Activities for Part I:
Analysis of the case:
1. Read A taste for Trouble Part I out loud to the class before you hand out or digitally
send copies to each student. (5 minutes to read Part I and give instructions for #2)
Rationale: Reading out loud allows all of the students to get the information at the same time
and helps auditory learners as well as slower readers get overall information. Students love to
be read to and this short scene is a perfect opportunity to get everyone on the same page, so to
speak!
2. Have students discuss the story with their partner and list clues found in the story
leading to a potential problem each girl might have. Combine everyone’s ideas into a list
entitled “What do we know?” for the class using an overhead projector, LCD or
chalkboard. Stress that they are looking for information not interpreting its meaning
when eliciting the list. (3-5 minutes to discuss, 5-10 minutes to make class list and give
instructions for #3)
Rationale: Students generally work better in pairs for each person is more active in the
endeavor and their ideas will more likely be discussed. Bringing everyone’s ideas together will
help focus the class if some students did not find all the clues.
3. Using the class list have students do an internet search on the girls’ symptoms to see if
they can narrow down what health issues are being presented. A questionnaire is
included to help guide them in thinking about the clues. (20 minutes or longer
depending upon class)
Rationale: Unraveling the health mystery on their own encourages students to be problem
solvers and to develop critical thinking. Piquing their interest by not answering questions but by
presenting them with the story, having them come up with the questions and seeking the
answers puts them in charge of their own learning.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Session 2: A Taste for Trouble Part II
Katrina’s cousin, Nicole, a local university student majoring in nutrition and fitness local
has decided to help “fix” Katrina’s health issues. Read A Taste for Trouble Part II to the class.
Nicole has given Katrina homework which becomes homework for your students. They must
first calculate Katrina’s BMR, SDA and Activity Calories to determine the number of Calories she
should consume each day to maintain her present weight. Using Katrina’s homework as an
example they determine their own BMR, SDA and Activity Calories. Since Katrina is asked to
keep a food diary, students are also asked to record their food and beverage intake for a period
of 3 days. Upon completion of the three days, students are asked to determine how close their
actual Calorie intake is to their calculated daily value.
Activities for Part II:
1. Read aloud A Taste for Trouble Part II before you hand out or digitally send copies
to each student. (5 minutes to read part II)
Rationale: Reading out loud allows all of the students to get the information at the same time
and helps auditory learners as well as slower readers get overall information. Most students
love to be read to and find out more about Katrina’s story.
2. Ask students their thoughts on Katrina and her problems. Have they learned
anything new about Katrina? (5-10 minutes to discuss the reading)
Rationale: A brief class discussion will focus everyone on the story. It will also give students an
opportunity to bring forth issues and concerns they may have about health and nutrition. Some
students may have similar issues or friends and family members with similar problems to
Katrina’s.
3. Show the food label slides (1 - 9) from “The Taste of Trouble” PowerPoint. Discuss
with students how to read a food label. Leave the slide up on how to read a
nutrition label. Have them complete the Reading a Nutrition Label worksheet in the
student manual. The second part of the activity requires them to use the nutrition
labels they have brought to class. Walk around the class facilitating the activity. End
the class by having students share some of their labels and information and turning
in their work. (1-2 class periods)
4. Students learn to determine Daily Calorie Requirements by first doing Katrina’s
Homework and then calculating their own Daily Calorie Requirements. (1 class
period)
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Rationale: Having a training session to do the calculations using common information in class
(Katrina’s Homework) will help them apply the technique to doing the calculations on the
information about themselves.
5. Students view PowerPoint slides on Nutrition Labels and then answer questions
about a simulated nutrition label. Using labels brought from home, they use the
knowledge learned to compare three of the labels and analyze the nutritional value
of each food. (1 class period)
Rationale: A training session followed by practice encourages skill building. Comparisons and
analysis of nutrition labels shows mastery of the skill.
6. After students have kept a 3 day food diary ask them to use the internet and other
resources to determine the number of calories they ate per day and how those
calories compare to their Daily Calorie Requirements. Encourage them to get calorie
(and other information for the advanced diary) from packages as they eat. On the
fourth day give them time in class to research their calories or assign the completion
of the food diary as homework. (1 class period or homework)
Rationale: Applying the information and skills to their own lives makes the lessons more
personal and impactful. Using skills in reading nutrition labels reinforces the skill while helping
them analyze their diets.
Session 3: A Taste for Trouble Part III
While walking in the park with her cousin, Katrina developed a blister which has failed
to heal. She ends up with an infection and a trip to the doctor. Her new doctor tells her frankly
she is morbidly obese and her weight may shorten her life. He tells her the blister may have
saved her life. Based on an in office urinalysis he requested a fasting blood sugar be done
immediately along with other tests. He plans to send her to a nutritionist to help design a diet
to help her lose weight.
Based on Katrina’s lab results, students are asked to determine what illness Katrina has.
Activities for Part III:
1. Ask students with their partners to look at the lab report from Katrina’s trip to the
doctor. Using the information given along with the information from Session 1, ask if
they are ready to make a diagnosis of Katrina’s major health issue. (10 minutes)
Rationale: Students need practice drawing conclusions. Gathering information and determining
the meaning of the information is the culminating practice in scientific methodology. Many
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
students can carry out the steps of an experiment but fall short when it comes time to draw
conclusions. Katrina’s story gives data to digest (pun intended!) and to make judgments about
what it might mean. It also gives practice in interpreting tables and charts which is always
helpful in writing lab conclusions and on standardized tests.
Session 4: A Taste for Trouble Part IV
Katrina has an appointment with Sharon, the diabetes consultant, at the local hospital.
Sharon gives Katrina reference materials about nutrition and diet. Typically a diabetes
consultant would give a client all of the information and not ask them to seek it out or do labs.
Most hospitals and clinics do not have facilities to have clients carry out lab experiments but this
scenario leads students into the Macromolecule ID assay training lab, the Training lab for
Determining Calories in Food, “Super Size Me!” video and the McMush lab done in her biology
class at school. This part of the case study contains most of the labs and activities.
Activities for Part IV:
1. Show students PowerPoint for A Taste for Trouble slides 21 – 23 on Macromolecules
101 and discuss the concepts. Students should take notes. (1-2 class periods)
Rationale: Going over information with students gives them a basis for understanding a
concept. Most students need repeated exposure and practice with a concept before they truly
understand it. Many times it is a simple activity or statement that gives a student the “Aha!”
moment.
2. Have students use Macromolecules 101 saccharide models to visualize dehydration
synthesis and hydrolysis and answer questions in Macromolecule 101 kits. Introduce
Training lab and go over the protocol. (1 class period)
Rationale: Many students are visual and kinesthetic learners. Holding the model in their hands
and manipulating the beads often provide a concrete example of an abstract process.
3. Training Lab – Identifying Organic Compounds in Food
This is referred to as a training lab because it is designed to lead the students in
conducting an assay to determine the purpose of each indicator solution. They
know what substances the food solutions contain, but they do not know which reagent
is an indicator for those substances. They develop a rubric for the testing solutions by
doing the lab and recording the results. After conducting the training lab they are
prepared to test different food products for the presence of starch, glucose and protein.
(2 class periods)
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Rationale: Students remember and understand information at a deeper level when they
construct the knowledge themselves.
4. Putting it Out of Proportion – perceptions about appropriate food portion size
Introduction: Give students a blank piece of paper and have them draw a typical
restaurant meal to scale. If they choose steak, how big a piece would they get? How
many French fries? How big is the bun for a hamburger?
a. Explain to students that over 60% of Americans are overweight. Ask students why
they think this might be the case.
b. Go to http://hp2010.nhlbihin.net/oei_ss/menu.htm#sl2 and show Portion
Distortion I Slide Show and; if time allows; Portion Distortion II Slide Show on the
National Heart Lung and Blood Institute website. Ask students their thoughts about
portion size keeping in mind their age and experience with portion sizes in
restaurants and fast food places.
c. View A Taste for Trouble PowerPoint slides 21-23 about portions size and discuss
with students how to relate portions to everyday items so they can estimate
servings.
d. Reflect: Have students revise their original drawing, considering the new
information about portion size.
Rationale: Students use prior knowledge and experience to make choices. Giving them new
data sets can help train them to make better food choices in the future. Cleaning your plate
may need to become a thing of the past especially in light of the bigger portion sizes served in
restaurants. Students need practice in estimating sizes based on everyday objects a skill useful
in science as well as in filling their dinner plate.
5. Watch Super Size Me! Morgan Spulock’s documentary on the fast food industry and
portion size. Stop the video occasionally and discuss with the class what is taking place.
a. Ask students to write a one page response to the video. Remind them you are
expecting their reaction to the video not a summary of what happened. It is
helpful to ask them to consider the following questions: How did this video
impact them? Do they eat fast food? If they do, will they eat it as often as they
did? What surprised them most in the video? Did they want to go to lunch at
McDonalds? If they do go to McDonalds will they make different choices on the
menu because of the video? (3 class periods)
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Rationale: This video has become a classic about the fast food industry and the individual’s
responsibility to educate themselves about health risks and relying on advertising to make
personal choices about diet. This video emphasizes the lifestyle causing Katrina to gain weight
and become ill. It also leads to the labs in biochemistry.
6. McMush Lab – A Happy meal is blended (without the toy!) analyzed using
skill sets learned in the Macromolecule ID. (2-3 class periods depending upon
equipment)
Rationale: This is a very impactful lab. Students are more likely to remember something they do
rather than see. They practice lab and science process skills, and it is interesting and surprising
for them to discover what is in fast food product they have been learning about.
Session 5: Katrina uses her new found knowledge to help her friend Jeri. She encourages Jeri to
eat better by following her example and seek information about her condition by going to the
doctor. This session introduces the global impact of diabetes in developing countries by giving
Katrina and Jeri an idea about doing their big class project; researching the changes in global
economy and the prevalence of nutritional diseases.
Activities for Part V:
1. Nutrition and Disease in a Global Community
Research Project – human health is impacted by nutrition. Have student use both Jeri
and Katrina’s ideas for a project and research nutritional based diseases. Then
investigate how world health is changing because of the changes in nutrition. (2-3 days
in class and additional time as homework)
Rational: Linking knowledge in individual research reinforces learning. Student interest is
piqued by Katrina’s and Jeri’s health issues to investigate further. Learning about nutrition
should be reflected in future choices in food and concern about how food impacts health.
Researching changes in world health as countries adopt a US diet and consequently suffer from
diseases such as diabetes and heart problems should highlight the problems with choosing to
eat the convenient yet unhealthy highly processed “foods” that have become a staple in our
everyday lives.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Background InformationCarbohydrates, lipids, proteins, and nucleic acids are organic molecules found in every living organism. These macromolecules are large carbon based structures. The macromolecules are assembled by joining several smaller units, called monomers, together through a chemical reaction called dehydration synthesis. The resulting polymer can be disassembled through the complimentary process called hydrolysis.
Carbohydrates are made of carbon, hydrogen and oxygen atoms in a 1:2:1 ratio. This means that for every carbon atom present in the carbohydrate there are two hydrogen atoms and one oxygen atom present. Greater energy containing double bonds exist between many of the carbon atoms in lipids. The monomers for carbohydrates are referred to as monosaccharides. When many monosaccharides are chained together the resulting molecule is called a polysaccharide. Carbohydrates are used by living organisms as an important source of energy. Examples of carbohydrates include glucose, fructose, sucrose, galactose, ribose, deoxyribose, cellulose and chitin.
Lipids are also made of carbon, hydrogen and oxygen but the ratio of carbon, hydrogen, and oxygen is not 1:2:1. Instead, lipids have a much higher number of carbons and hydrogens with few oxygen atoms present. The nonpolar bonds that form between the carbon and hydrogen atoms of a lipid cause them to be hydrophobic, or water-repellent, molecules. This explains why water and oil do not mix. The large number of carbon to hydrogen bonds also serves to make lipids energy rich storage molecules. One gram of lipid stores twice as much energy as one gram of a carbohydrate. Lipids from animals are referred to as fats and are solids at room temperature, while those found in plants are referred to as oils which are liquids at room temperature. Fats and oils are made of smaller units called triglycerides which are composed of a glycerol and three fatty acid molecules. One important relative of true lipids are the phospholipids. Phospholipids differ in structure from regular lipids in that phospholipids are made of a glycerol and two fatty acids joined by a phosphate group. This arrangement makes phospholipid molecules have both hydrophilic and hydrophobic regions. This feature makes phospholipids an ideal structural component of the plasma membrane of cells. Steroids are another significant group of lipids.They differ slightly in structure because the carbon atoms are arranged in four rings. Examples of steroids include cholesterol, estrogen, testosterone and morphine.
Proteins are composed of long folded chains of amino acids which are composed of atoms of carbon, hydrogen, oxygen and nitrogen. Proteins serve as the major building blocks of organisms. Proteins are large complex molecules that combine to form various components of living organisms such as muscle fibers, enzymes, and hemoglobin. Proteins are made of unique combinations of the twenty amino acid monomers. A string of amino acid monomers joined together by peptide bonds is called a polypeptide.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Vitamins and Minerals Reference Sheet Vitamins are molecules needed in small quantities for proper growth and activity. Humans cannot make many of the vitamins themselves and must eat them in their diet. Essential to life/Important in helping with cell chemistry (metabolism); they help enzymes to make
many of our cell’s reactions work correctly! From G. & C Ulmer
Vitamins
Name A Few of their Functions
A Aids in normal night vision, healthy skin, immunity against diseases
B1 (thiamine) Aids in the breakdown of sugars in cells, proper digestion, and appetite
B2 (riboflavin)Aids in red blood cell production, breakdown of sugars, fats, and proteins
in cells
B3 (niacin)Aids in proper digestion, healthy skin, breakdown of sugars, fats, and
proteins in cells
B5 (pantothenic acid)Aids in proper digestion, healthy nervous system, breakdown of sugars,
fats, and proteins in cells
B6 (pyridoxine)Aids in red blood cell production, fat and protein absorption, normal brain
function, and immunity against diseases
B12 (cyanocabalamin)Aids in red blood cell production, protein chemistry in the cell, healthy
nerve function
Folic AcidAids in red blood cell production, immunity against diseases, and normal
cell division
Choline Aids in the breakdown of fats in cells, proper nerve functions
BiotinAids in fat chemistry in the cell, breakdown of sugars in the cell, and
healthy skin and hair
C (ascorbic acid) Aids in disease resistance and wound healing
D Aids in normal tooth and bone development
E Aids in keeping cells from being damaged and wound healing
K Aids in blood clotting and bone formation
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Minerals Minerals are ions we need in small amounts to function properly. We must get all of our minerals by eating them in our diets (mainly by eating plants). Essential to life/ Important in our body fluids/ Important in helping with cell chemistry.
Minerals
Name A Few of their Functions
CalciumNeeded for tooth and bone formation, muscle contraction, and blood
clotting
Chloride Aids in regulating water concentrations in and out of our cells
Cobalt Aids in the production of several important enzymes
Copper Aids in bone formation, nerve function, and red blood cell formation
IodideRegulates the rates of body reactions, and aids in proper thyroid gland
function
IronNeeded by red blood cells to pick up oxygen, works with many important
enzymes in our cells
MagnesiumHelps enzymes break down sugars, fats, and proteins for energy, needed
for proper nerve and muscle function
Manganese Aids in breakdown of proteins and fats, bone and cartilage formation
Phosphorus Aids in tooth and bone formation
PotassiumNeeded for nerve function, regulates water consumption in and out of our
cells
SodiumNeeded for nerve function, regulates water consumption in and out of our
cells
SulfurNeeded to produce some of our amino acids, helps protect cells from
damage
ZincWorks with many important cell enzymes, helps protect cells from
damage, aids in bone formation
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Teaching ResourcesA Taste for Trouble Power Point is included with this Case Study. Slide numbers are indicated in the lesson plan and each section has a title slide for ease of use.
Several websites are listed in references invaluable as resources for both class presentation and student research.
Distortion Proportion Slide Show I and II can be accessed on line here:
National Heart, Lung and Blood Institute Obesity Initiative Slide Show Sets: http://hp2010.nhlbihin.net/oei_ss/menu.htm#sl2
The textbook used in biology class is an excellent resource and should not be discounted while doing this Case Study. While this “A Taste for Trouble” could stand alone it is an excellent addition to the program of study already incorporated into your curriculum.
Materials and Possible Venders:Reagents and other lab materials may be purchased at any lab company. Below are some suggested companies.
Flinn Scientific, Inc. Toll Free: 1-800-452-1261P.O. Box 219 Fax: 1-800-452-1436Batavia, IL 60510 email: [email protected]
Carolina Biological Supply Company Toll Free: 1-800-334-55512700 York Road, Burlington, NC 27215-3398
WARD'S Natural Science Toll Free: 800-962-2660 PO Box 92912 Rochester, NY 14692-9012
Craft materials may be purchased at Wal-Mart, Hobby Lobby, Michael’s or any craft store.
Macromolecules 101Plastic Needle point canvasPipe cleaners (any color)Black and white pony beads
Safety equipment for labs include: goggles, aprons, glovesTraining Lab - Macromolecule ID Equipment to Prepare:
6 – 13 mm x 100mm test tubes placed in a 250ml beaker/grouptransparent tape and waterproof pen
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
1 50o C hot water bath/group (hot plates with 250 mL beakers of water work well)18 small beakers or containers 18 labeled eye droppers or disposable pipettes Biuret SolutionBenedict’s SolutionLugol’s Solution
Solutions to Prepare:
1. Biuret, Benedict’s, and Lugol’s solutions are poured from their shipping bottles without dilution.
2. Distilled Water Food Solution – tap water works fine3. Sucrose Food solutions – sucrose doesn’t react with any of the test solutions so it’s
easiest to cheat and use plain water for the sucrose solution. Otherwise, add one heaping teaspoon of table sugar to 500 mL of water.
4. Concentrated Glucose Food Solution – add 40 mL of clear corn syrup to 500mL of water5. Dilute Glucose Food Solution – add 10 mL of the Concentrated Glucose Solution above
to 500 mL of water6. Starch Food Solution – use a can of laundry spray starch from the store and spray for 15
seconds into a beaker filled with 500 mL of water OR put a teaspoon of cornstarch into 500 mL of water and mix well.
7. Protein Food Solution – stir .5 of a heaping teaspoon of powdered albumin into 500 mL of water
500 mL of each organic food solution should be more than enough for 5 classes.
McMush LabMcDonald's value mealBenedict's solution- tests for sugar (carbohydrates)Biuret solution- tests for proteinLugol's iodine solution- tests for starch (complex carbohydrates)Silver NitrateBrown paper (craft paper or a brown paper bag)Kitchen Blender1 hot plate1 - 500 ml beaker1 - 200 ml graduated cylinder100 ml of water2 oven mitts1 wooden spoon1 refrigerator to cool McMush mixture (although you could use an ice chest if you had no access to a refrigerator)
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
A Taste for Trouble: Part I
Katrina huffed down the hall. She was pooped! It was just second period but it had
already been a long day finding her way around the unfamiliar high school. It was bad enough
to move to a new town right before her sophomore year but it seemed all her classes were as
far apart as humanly possible. She found room 128 and squeezed her large frame into the first
available seat. She would rather have sat at the back where she might escape notice, but being
in front she might actually make a badly needed restroom break before she made her way
across this ridiculous building to her Spanish class.
A skinny blonde slipped into the chair next to her. “I am so hungry I am about to pass
out!” She exclaimed. “You wouldn’t have anything to eat in that big bag of yours would you?”
She eyed Katrina’s enormous purple purse.
“I do keep a stash of candy bars,” Katrina confessed. “I’m usually hungry myself.” She
rummaged in her bag and handed the girl a chocolate candy bar. “Not to mention thirsty! I am
Katrina by the way.”
“Jeri” mumbled the girl through a mouthful of candy, “nice to meet you.” She
swallowed, “Thanks for saving me. I heard they have vending machines in the building but I am
still trying to find my classes and I know I will never see my locker again. Why does everything
have to look the same?”
“I know,” sighed Katrina. “If they ever take down that gruesome science poster at the
front of this hall I will never find my way back to Kansas!”
Jeri did a double take as she took another bite of candy, “Gosh! Are you from Kansas
too?”
“No, I am from Missouri, I was referring to the Wizard of Oz, not that I am Dorothy or
anything but moving from St. Louis to this little town out in the sticks is like being plunged into
the land of the munchkins. No offense, but I prefer a larger town where they have more fast
food choices and shopping malls. Are you really from Kansas?”
Several students careened into the room seconds after the tardy bell disgustedly looking
at the remaining seats.
“OK, class! Let’s get started!” The teacher’s voice startled the class into attention.
“Those who have just come in don’t worry about hunting for the perfect spot. I am going to
assign seats for the first few weeks anyway.” Audible groans from the teens. “Also, I won’t be
counting you tardy today but I will tomorrow so get here on time! It also looks like some folks
21
Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
have forgotten the “no food in class” rule from your handbooks! Remember to save your snacks
for the nutrition break after 3rd period.”
Jeri quickly slid the remaining candy bar down beside her on the seat. “Tell you later,”
she mouthed to Katrina, who had crossed her legs and wished her last name was not so far
down in the alphabet. She hoped this class would go by quickly! Her shot at making it to the
small overcrowded girl’s restroom during passing time faded with the seating chart, never mind
getting a much needed bottle of water. She would just have to be late next period. Perhaps her
Spanish teacher would also be forgiving about tardiness on the first day of school.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Activity Sheets for A Taste for Trouble Part IFrom the story, generally describe the body type of each girl:
Katrina: ________________________________________________________________________
Jeri: ___________________________________________________________________________
What do you know about Katrina’s food preferences from the story? ______________________
______________________________________________________________________________
What physical symptoms does Katrina have (think about what is making her uncomfortable in the scene)? ______________________________________________________________________________
______________________________________________________________________________
What physical symptoms does Jeri have? ______________________________________________________________________________
______________________________________________________________________________What do you know about Jeri’s eating habits from the story? _____________________________________________________________________________
Internet Research: For girl and each symptom, make a list of possible causes.
Katrina:
Clue #1: Clue #2: Clue #3 : Clue #4:
What questions would you ask Katrina to help you narrow down her possible health issues?
If you were her doctor what type of tests would you request in order to diagnosis Katrina?
Jeri
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Clue #1: Clue#2:
What questions would you ask Jeri to help you narrow down her possible health issues?
If you were her doctor what type of tests would you request in order to diagnosis Jeri?
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
A Taste for Trouble: Part II“I could wring her scrawny little neck!” exclaimed Katrina as she flopped down on the
sofa, setting her Big Gulp cup on the ottoman in front of her while blowing her bangs out of her
eyes. She let her heavy backpack slide to the floor off her shoulder as she struggled to untie her
Nike’s and untangle her purse all at the same time. “She has no idea what it is like!” she ranted.
“What are you complaining about now!” asked her mother from the doorway. I am
barely in the house before the griping begins. I understand how hard it is to switch schools
when you are a teenager but give me a break already!” Moving hasn’t been a walk in the park
for any of us.” Katrina rolled her eyes at her mom’s remarks.
Her mother changed her tone, hoping to prevent more drama. “Did you see Nicole
today? She was so excited we were moving to town.”
“If you’re trying to change the subject, you just failed,” snorted Katrina. “She is the one
whose scrawny neck I was eager to wring!”
“Oh! Katrina! That is not very nice. She was always your favorite cousin and you were
happy she was at Central University!” returned her mother. “What was it you said? Her
presence was the only saving grace in moving to this one-horse town or some such thing!”
“Was, is the operative word! When I met her in the Student Union, she had the gall to
gasp and exclaim about how much weight I gained since last Christmas; AND then she went on
and on about how she was going to get me into shape. Like she is so perfect! I could snap her in
half like a twig! She went on and on about eating right and exercising until I thought I would
barf! She expects me to go along with all of this. She may be skinny but she doesn’t know
anything about manners! You don’t go around telling people they are fat; even if they are!”
“Well, honey, you know she is majoring in nutrition and fitness. It is only natural she
would get excited about helping you.” replied Katrina’s mom.
“I don’t remember asking for help! But Miss Smarty Pants gave me homework to do! I
am supposed to find my height and weight and use this stupid formula to figure out I am fat! I
know I am fat! Good Grief! I am so tired I want to go to bed right now and I have 6 tons of
stupid homework and plenty of it is math without fat formulas on top! Geez! And she wants
me to meet her at the park so we can work out together. So yeah, moving here looks like it is
turning in to lots of walks in the park! I don’t have enough energy to do anything and she wants
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
me to do more! That makes perfect sense! NOT!” She stood up and took a big swig of her soda
as she headed down the hall to the bathroom.
“Well, dear” ventured her mom, “once you get a ton or two of the homework done, you
can set the table for dinner. I got rid of some boxes so we can finally sit down and eat like an
actual family tonight.”
“Great!” retorted, Katrina. “My last meal before I am doomed to count every blessed
calorie for Miss Prissy Pants!”
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Activity Sheets: A Taste for Trouble Part IIKatrina’s Homework
Katrina must find her height and weight. Luckily they have an accurate bathroom scale she can use at home. Her mom helped her find her height by using a ruler, a pencil and a tape measure. Katrina stood straight with her back against the wall and her mom put the ruler on top of her head at a right angle to the wall. She made a small mark with the pencil which could easily be erased later. They used the tape measure to measure from the floor to the mark. Katrina is 5’5” tall and weighs 295 pounds. She is 16 years old. Nicole wanted her to use one of the following formulas:
English BMR FormulaWomen: BMR = 655 + ( 4.35 x weight in pounds ) + ( 4.7 x height in inches ) - ( 4.7 x age in years )Men: BMR = 66 + ( 6.23 x weight in pounds ) + ( 12.7 x height in inches ) - ( 6.8 x age in year )OrMetric BMR FormulaWomen: BMR = 655 + ( 9.6 x weight in kilos ) + ( 1.8 x height in cm ) - ( 4.7 x age in years )Men: BMR = 66 + ( 13.7 x weight in kilos ) + ( 5 x height in cm ) - ( 6.8 x age in years )
Calories are needed to chew and digest your food. This is referred to as Specific Dynamic Action or SDA. It is figured as 10% of your Basal Metabolic Rate (BMR) SDA = BMR x .1
Specific Dynamic ActionSDA BMR x .1
To determine your total daily calorie needs, multiply your BMR by the appropriate activity factor, as follows:
Activity Factor Calorie-CalculationIf you are sedentary (little or no exercise) BMR x 1.2If you are lightly active (light exercise/sports 1-3 days/week) BMR x 1.375If you are moderately active (moderate exercise/sports 3-5 days/week) BMR x 1.55If you are very active (hard exercise/sports 6-7 days a week) BMR x 1.725If you are extra active (very hard exercise/sports & physical job or 2x training) BMR x 1.9
Katrina’s BMR = 655 + (4.35 x _________lbs.) + (4.7 x _______”) – (4.7 x ____years old)
655 + ________+ ________ - __________ = __________
Katrina’s SDA = BMR x .1 __________x .1 = ____________
Katrina’s Activity Calories = BMR x 1.2 __________x 1.2 = _____________Cal
Katrina’s Results (round the Total to the nearest whole number)
Katrina’s BMRKatrina’s SDA
Katrina’s Activity CaloriesTotal # of Calories/Day
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Find Your Calorie Needs
Use the same information Katrina used in her homework to calculate your BMR, SDA and Activity Calories.
Your Height ___ft. ____in. = _____in x 2.54 = ______ cm Your Weight lbs. Kg
Convert feet into inches by multiplying by 12.English Basal Metabolic Rate (BMR) FormulaWomen: BMR = 655 + ( 4.35 x weight in pounds ) + ( 4.7 x height in inches ) - ( 4.7 x age in years )Men: BMR = 66 + ( 6.23 x weight in pounds ) + ( 12.7 x height in inches ) - ( 6.8 x age in year )OrMetric Basal Metabolic Rate (BMR) FormulaWomen: BMR = 655 + ( 9.6 x weight in kilos ) + ( 1.8 x height in cm ) - ( 4.7 x age in years )Men: BMR = 66 + ( 13.7 x weight in kilos ) + ( 5 x height in cm ) - ( 6.8 x age in years )
Calories are needed to chew and digest your food. This is referred to as Specific Dynamic Action or SDA. It is figured as 10% of your Basal Metabolic Rate (BMR) SDA = BMR x .1
Specific Dynamic ActionSDA BMR x .1
To determine your total daily calorie needs, multiply your BMR by the appropriate activity factor, as follows:
Activity Factor Calorie-CalculationIf you are sedentary (little or no exercise) BMR x 1.2If you are lightly active (light exercise/sports 1-3 days/week) BMR x 1.375If you are moderately active (moderate exercise/sports 3-5 days/week) BMR x 1.55If you are very active (hard exercise/sports 6-7 days a week) BMR x 1.725If you are extra active (very hard exercise/sports & physical job or 2x training) BMR x 1.9
Your BMR = 655 + (4.35 x __________lbs.) + (4.7 x __________”) – (4.7 x _____years old)
655 + ________+ ________ - __________ = __________
Your SDA = BMR x .1 __________x .1 = ____________
Your Activity Calories = BMR x 1.2 __________x 1.2 = _____________Cal/day
Your Results (round the Total to the nearest whole number)
Your BMRYour SDA
Your Activity CaloriesTotal # of Calories/Day
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Reading Nutrition LabelsUse the nutrition label to answer the following questions.
What food item does this nutrition label describe? _________________________________________ 1. What is the serving size? __________. How many
servings are in the container? ___________
2. How many Calories are in each serving? _________. How many Calories are from fat? ___________
3. Total fat = ________g Saturated Fat = ________g Trans fat = __________g Cholesterol = _________g Sodium = ________mg Total Carbohydrates = ______g Dietary Fiber = ______g Sugars = ________g Proteins =________g
4. Vit. A ____% Vit. C _____% Calcium _____% Iron _____%
5. Of all the ingredients listed on the label this food contains the most _________________
6. In terms of the % Daily Values of needed nutrients would this food be considered Low (5% or less) or High (20% or more) Explain________________________________________________________________________________________________________________________________
7. The Percent Daily Values (%DV) are based upon a ________________Calorie Diet. What % DV does this food item supply of dietary fiber?_____________% How many grams of Dietary Fiber are required for a person needing 2,000 Cal/day __________g and by a person needing 2,500 Cal/day __________g.If you needed 3,000 Cal/day what amount of dietary fiber should you have in your diet? ___________g.
Some amounts of certain nutrients go up with Calorie requirements. Which nutrient amounts stay the same regardless of Calorie needs? ___________________________________________
Different macromolecules are bonded together differently and have differing amounts of calories. Generally Lipids (fats) are good energy storage molecules because they have about 9 Cal/g, both Carbohydrates and Proteins have about 4 Cal/g. Complete the following table:
Beef Stew Soup 170 Cal/cup Protein 10g = ___________CalCarbohydrates 19g = _____Cal
Total fat 5g = ___________CalTotal Sodium 810 mg
Vegetable Beef Soup 120 Cal/cup
Protein 7g = ____________CalCarbohydrates 19g = _____Cal
Total fat 2g = ___________CalTotal Sodium 410 mg
Light Beef and Barley Soup 80 Cal/cup
Protein 5 g = ___________CalCarbohydrates 14g = _____Cal
Total fat 1.5g = __________CalTotal Sodium 410 mg
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Nutrition Label ComparisonGlue or tape three of your nutrition labels below. If the name of the food is not on the label, write it in the blanks labeled Food:Food A: Food B: Food C:
1. Which of these foods has the most Calories per serving? __________________________
2. Which of these foods has the least amount of total fat? __________________________
3. Which of these foods would be considered high in % Daily Value? __________________
4. Which of these foods has the most sodium? ____________________________________
5. Which of these foods would be the most nutritious? _____________________________
6. What did you choose this food as most nutritious? ______________________________________________________________________________________________________
7. Which food has the least saturated fat? _______________________________________
8. Which food has the most dietary fiber? _______________________________________
9. Which food would be the best choice if you had to limit your carbohydrates? ____________________________
10. Which of these foods would be the best if you had to limit your sodium intake? _____________________________
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Nicole also asked Katrina to keep track of the food she ate each day. She specifically asked her to also track the beverages she consumed, including water. Use the following to keep your own food diary.
Daily Food DiaryFood Day 1 Calories Day 2 Calories Day 3 CaloriesBreakfast
Snack
Lunch
Snack
Dinner
Snack
Total CaloriesCalculated Required Cal/DayDifference
Subtract the actual Calories you ate from your Calculated Required Cal/Day. If you ate more put a + sign in front of the number, if you ate less put a – sign in front of the number.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Advanced Diary: Students can also keep track of carbohydrates, proteins and fats and determine how many grams of each they eat each day.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
FOOD LABELS AND BIOLOGICAL MOLECULES
Alternate assignment sheet to track fat and protein along with carbohydrates
Make a list of all the food that you eat during a 3 day period. o If a food label is available use it to determine mass of carbohydrates, total lipids
(fats), and protein. o Information for foods such as meats and produce can be found on the internet
or in cookbooks. The following site is from the US Department of Agriculture which regulates labeling of foods.
http://www.nal.usda.gov/fnic/foodcomp/search/index.html
Go to the site, type in the name of the food, click “submit.” A list of foods matching the term will appear. Click on the one that is closest to
what was eaten. Scroll to the bottom of the page and click “submit.” Usually there is a couple of serving size choices. Unclick “100 grams” and select
what seems most appropriate, and click “submit.” Click “submit.” The next page contains a chart with more information than
needed. Refer to your chart to gather what is required for this assignment.
On a separate piece of paper, add each nutrient (total carbohydrate, protein, fat) and list the total grams in a chart.
Make a bar graph indicating the total grams of each nutrient. You will be graded on neatness and completeness, not on your dietary choices.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Advanced food Diary:
Food Diary
Food Approximate amount
Calories Total Carbs
(g)
Protein (g)
Total Fat (g)
Total Average per day
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
A Taste for Trouble Part III“Katrina Martin” stated the nurse, in the brightly patterned scrubs, as she opened the
door into the waiting room. “We are ready for you. Please come back with me and we’ll get
started.” She smiled as she held the door open for Katrina. Her smile faded as she watched
Katrina limp toward the door.
“Hi, Katrina, my name is Tracy. I will be helping Dr. Carter take care of you. I’ll put your
stuff in the exam room; you just head toward the scales.” She nodded down the hall as she took
Katrina’s purse and put it on a chair in a nearby exam room. She caught up with Katrina quickly
and helped her step onto the medical scales. “This will just take a second and we will get you off
that foot.” Tracy said in a business-like manner. “OK, let’s see.” She shifted the weights on the
scale swiftly and wrote 290 lbs. in Katrina’s chart.
“Wow! I lost 5 pounds!” exclaimed Katrina. Tracy did a double-take as she had been
expecting a groan. “Most people don’t get that excited about me taking their weight,” She
explained.
“Well, that would usually describe me too except I just started to work on losing weight
last week and am already making progress! I could really get into groaning about the remaining
290 pounds though if that would make you feel better!” exclaimed Katrina.
“No, no!” Tracy responded hastily. “I prefer my patients to be upbeat!” She pulled the
height bar up and marked down 5’5” in the chart. “Let’s get you back to the room and get your
pulse, blood pressure and temperature. But first, you get to pee in a cup!” She motioned
Katrina toward the restroom door. “All the supplies are on the shelf with directions on this wall
poster. Holler if you need me, I’ll be right here.”
Katrina emerged a few moments later presenting Tracy with the urine specimen as if it
were a gift. “Voila, Madame!”
“I am glad the pain in your foot has not spoiled your sense of humor!” laughed, Tracy.
“It is tough to be new in town but I bet you make friends easily.”
“I‘ve never really thought about it,” shrugged Katrina. “I’ve lived in St. Louis my entire
life so I don’t know if I am good at making new friends or not. I sure miss my old ones though.
Thank goodness for texting and Facebook!”
Back in the exam room Tracy quickly found Katrina’s vital signs and entered them into
the chart. She and Tracy went over the pre-exam history Katrina had filled out in the waiting
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
room. “I wish my mom could have come with me today,” sighed Katrina. “She is much better at
all these details.”
“It would have been best if she was here but we will be getting your medical
information from your doctor in St. Louis and we will be calling her to fill in the blanks. Now, tell
me what happened to your foot,” said Tracy, kindly.
“Well, I blame my cousin Nicole” laughed Katrina. She went on to explain that in trying
to help her lose weight and get healthy, her favorite cousin Nicole had gotten Katrina to walk on
the trail in the local park three times a week. Her mom wanted to be supportive of the effort
and bought Katrina a new pair of walking shoes. Unfortunately she rubbed a blister the first day
and it hadn’t healed. “Nikki’s efforts at getting me fit seem to have backfired. Now it is hard to
walk at all!” explained Katrina.
Tracy asked Katrina more questions as she made notes in her chart. “Well. We have
arrived at the portion of our program where Dr. Carter is required.” Tracy handed Katrina a
couple of magazines. “I shall leave you for the moment to catch up with fashion and such. Dr.
Carter will be here in a jiffy!” smiled Tracy as she gently closed the exam room door.
A few minutes later there was a knock at the door and a tall, brown haired man opened
the door as Katrina said, “come in?”
“Hi, Katrina, I am Dr. Carter,” greeted the man. Tracy followed him in. “I thought I
would stay since Katrina’s mom couldn’t be here.”
Katrina smiled at her gratefully as she sat the magazine aside. She was a bit nervous,
she admitted to herself. “Katrina,” began Dr. Carter after swiftly performing some quick
assessments by looking in her throat and nose, feeling her neck and listening to her heart and
breathing with his stethoscope. “I know we have just met and this may seem very harsh but I
need to be very direct with you. You are morbidly obese and in danger of a variety of diseases,
all which could shorten your life.”
“That is certainly direct!” exclaimed Katrina. “I knew I hadn’t felt good in a while but I
didn’t think I had anything that losing some weight and exercising wouldn’t fix. I thought my
main problem was the sore on my foot and you haven’t even looked at that yet.”
“The sore, while painful, is just a symptom of a bigger problem.” He said as he gently
peeled the bandage from her foot. “I see flip flops have been the choice of footwear.”
“All my other shoes rub the bandage off.” She replied. “Luckily, I have several pair!”
What do you think is wrong with me?” she asked nervously.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
“Well,” Dr. Carter responded, as he cleaned her sore with antiseptic, applied an
antibiotic ointment and re-bandaged her foot, “I think you may have a problem stemming from
your weight and lifestyle choices and genetics may play a part too. Your pulse and blood
pressure are also higher than they should be. The urinalysis we did suggests your glucose levels
are way too high. I am going to get some lab work done to find out for sure. We will make
another appointment when the results come in and I will be able to make a more certain
diagnosis. I will want your mom to come with you so we can talk about everything together.”
“I know what I said to you was shocking, but I want you to know how serious your
situation is. The blister on your foot may well have saved your life because it got you in to see a
doctor. One of the studies I am asking you to do is called a fasting glucose test. You will have to
miss a day of school and won’t be allowed to eat anything for 8 hours before the test. They will
draw a blood sample and then have you drink some glucose and after a bit they will take
another blood sample. I will also have the lab analyze other aspects of your blood to determine
how well your liver, kidneys, thyroid and other organs are functioning along with cholesterol
and triglyceride levels.” Dr. Carter explained. “Do you know what those are?” he asked?
“I have an idea,” she replied. “We are starting to study macromolecules in biology this
week so I know I will be learning more.”
“That is good,” he replied, “Knowledge is power!” While what I said about your health is
very serious the good news is your outcome can change for the better with the right treatment
and life-style changes.” He paused for a moment to wash his hands.
“From the looks of your foot you were pretty dedicated to exercising with your cousin.
We need to make sure you are healthy enough to continue your exercise program. I will give
you a prescription for some oral antibiotics along with this ointment,” he said as he handed her
the tube. “Keep the wound covered while you are at school so it will stay clean but if you are
home, prop your foot and take the bandage off.” Katrina nodded her understanding as she took
the tube of ointment.
You will definitely need to change your diet too. Tracy tells me you have lost some
weight. What have you done to lose the five pounds?”
“Well, I stopped drinking sodas and starting drinking water instead. I thought I would
start small and work towards changing my diet. I figured I would get discouraged if I couldn’t
eat some of the things I enjoy. I was going to give up my afterschool candy next,” offered
Katrina.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Dr. Carter smiled at her, “That’s a great start! I am going to suggest some other changes
too and will be sending you to see a nutritionist who can help you plan a better diet. Once we
determine what your health situation is we can get you feeling better and you will enjoy being
more active.”
Tracy turned to Katrina after Dr. Carter left the exam room, “are you ok? That was pretty
tough to hear.”
Katrina smiled back at her thoughtfully. “It is like I told my mom the other day. I know I
am overweight and have health issues. Dr. Carter just told me straight up I will have to deal with
them or have a short life. I am glad he was so direct and didn’t try to make me feel better about
my weight. It would have been easier to keep kidding myself that it is OK to weigh almost 300
pounds at my age but it wouldn’t have helped me in the long run.”
Tracy gave her a quick hug after handing her the information sheet for the lab and a
new appointment card. “You are a very brave kiddo” she said. “I’ll see you on Thursday!”
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Activity Sheets: A Taste of Trouble Part III:
Lab Results and Normal Range Information
Fasting Blood GlucoseGLUCOSE LEVEL INDICATION
From 70 to 99 mg/dL (3.9 to 5.5mmol/L Normal fasting glucoseFrom 100 to 125 mg/dL (5.6 to 6.8 mmol/L Impaired fasting glucose (pre-diabetes)126 mg/dL (7.0 mmol/L) and above on more than one testing occasion Diabetes
Oral Glucose Tolerance Test (OGTT)Levels applicable except during pregnancy. Sample drawn 2 hours after a 75-gram glucose drink.Less than 140mg/dL (7.8 mmol/L) Normal glucose toleranceFrom 140 to 100 mg/dL (7.8 to 11.1 mmol/L) Impaired glucose tolerance (pre-diabetes)Over 200 mg/dL (11.1 mmol/L on more than one testing occasion Diabetes
American Association for Clinical Chemistry
Health Links Inc. 100 Somewhere Road Small Town, OK 12345CLIA 35X2187908Patient: Katrina Martin D.O.B. 06/08/1994 16Y/F Pt. # 987654321 Acct# A045320988215Physician: John Carter Acct # 45-455610Date: September 14, 2010Specimen Received Date and Time: September 14, 2010 10:00 AMChemistry
Name Result Flag Normal RangeGlucose 302c* (C) 70-110 mg/dLSodium 149 (H) 135 – 14 mEq/LPotassium 4.2 3.5-5.5 mEq/LChloride 95 (L) 96-113 mEq/LCO2 25 21-34 mEq/LBUN 11 6-23 mEq/LCreatinine 1.1 (H) 0.2 – 0.6 mEq/L
C* = comment C= Critical H= High L=Low D=Delta
c* Critical High Glucose value called to Dr. Carter’s office. Results given to nurse Tracy HowardJohn Q Tech, MT Sept 14, 2010 10:50 AM
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
A Taste for Trouble Part IVKatrina knocked on Sharon Smalley’s office door. “Come in!” shouted Sharon. Katrina
poked her head around the door to see Sharon teetering on a stool as she reached up to the top
shelf of her office bookcase.
“Well that certainly looks dignified!” exclaimed Katrina. “What if I was the president of
the hospital or something!”
Sharon laughed as she caught hold of the book she had been trying to snag. “I wouldn’t
care. Maybe they would get me a better bookcase or catch me if I fell!” she exclaimed. “You
are looking healthier since the first time I met you. How are you feeling?”
“Oh tons better! My foot has healed and I am not as tired as I was. I sleep better too. I
don’t have to get up all the time for a drink or to use the rest room. Mom says the food bill is
way down too,” she giggled.
“That is good news! Tell me how checking your blood sugar is going? Are you finding a
good routine and not skipping any blood checks?” Sharon inquired as she settled back into her
desk chair.
“Yup! Although poking myself with needles several times a day is not my fav it sure
beats the way I had been feeling.” She sat her bag down and reached for the book Sharon was
handing her. “Gee whiz, this is a big book. Is lifting this several times a day my new exercise
program?”
“No silly! This is a little light reading for your spare time.” She smiled as Katrina looked
aghast! I am kidding, sort of. This is a really good reference book on biochemistry.” Katrina
looked more aghast if possible. “I used it in my college nutrition class and I thought I would lend
it to you for a while.”
“Gee, thanks!” Katrina responded slowly. Her mom had taught her to always be polite.
“Last time you were here you told me you were starting a unit about macromolecules at
school. This book has a lot of reference material in it you might find useful. It is well written
and explains things very clearly. I think you will find your diabetes to be more manageable the
more you understand what is happening in your body.” Sharon smiled at Katrina.
“I don’t usually go to these depths with a client but I feel you will benefit from understanding
the information on a deeper level. Your biology teacher is a friend of mine and I know she has
some labs planned and you have an excellent biology text. Understanding the biochemistry
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
helped me manage my own health when I was first diagnosed back in college. It also helped me
to decide what I wanted to do as a career,” she explained as she leaned back in her chair.
“I’m not sure I want to be a nurse,” said Katrina doubtfully as she looked down at the
book.
Sharon laughed, “I’m not trying to convert you to a new career path, just help you
educate yourself. The book is just a loner. I want it back when you are done.” She winked a
Katrina. “But take your time I am not eager to climb back up to put it away.”
“Now,” she said leaning forward and brushing her hair out of her eyes with the back of her
hand, “let’s talk turkey! Portion size that is!” she reached for a deck of cards lying on her desk.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Activity Sheets for A Taste for Trouble Part IV
Super Size Me! Video
Documentary Video: Rated PG-13 for language, sex and drug references, and a graphic medical procedure. (Original version): Rated PG for thematic elements, a disturbing medical procedure, and some language. (Educationally enhanced version)
Run Time = 100 minutes
Several legal suits have been brought against MacDonald's Restaurants that they are knowingly selling food that is unhealthy. Some of the court decisions have stated that the plaintiffs would
have a claim if they could prove that eating the food every day for every meal is dangerous. As such, documentarian Morgan Spurlock conducts an unscientific experiment using himself as the
guinea pig: eat only MacDonald's for thirty days, three meals a day. If he is asked by the clerk if he would like the meal super sized, he has to say yes. And by the end of the thirty days, he will
have had to have eaten every single menu item at least once.
Before starting the experiment, he is tested by three doctors - a general practitioner, a
cardiologist and a gastroenterologist - who pronounce his general health to be outstanding. They will also monitor him over the thirty days to ensure that he is not placing his health into
irreparable damage. He also consults with a dietitian/nutritionist and an exercise physiologist, the latter who also deems him to be above average fitness. As it mimics the lifestyle of those
who eat fast food, he will also do no exercise for the thirty days, limiting himself to under 5,000 steps per day (the approximate equivalent of 2½ miles). These health and medical experts have
some predictions about his general health and wellness by the end of the experiment. His vegan chef girlfriend also has some predictions about how this experiment will affect his mood and
therefore their relationship. As he goes through the experiment, he speaks to a number of people - many experts in their respective fields - on the pros and cons of the fast food lifestyle.
Just over halfway through the experiment, it is evident that even the experts can be wrong, and not in a good way. Written by Huggo
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Macromolecules 101
Bonding activity with plastic shapes, pipe cleaners and pony beads to model hydrolysis and condensation reactions.
This activity is done with plastic needlepoint canvas shapes, pipe cleaners and pony beads. Kits of these materials, along with a direction sheet, are made up and put into snack-size zip top bags and keep in a gallon size bag to be used over and over again.
Pieces of the kit
The white shape represents a glucose molecule with a formula of C6H12O6.
The maroon shape represents a fructose molecule with a formula of C6H12O6 Both glucose and fructose are single sugars called monosaccharides.The pipe cleaner represents the covalent bonds.The white beads represent oxygen atoms The black beads represent hydrogen atomsDirections: Take a hydrogen atom off of one molecule and a hydrogen and oxygen atom off of the other molecule. Link the two shapes together through the remaining oxygen atom. Answer the following questions on a separate sheet of paper.
1. What small molecule can you make with the beads you took off each sugar molecule?
2. The fructose and glucose have been linked together. They were monosaccharides. Now they form what kind of saccharide?
3. How many bonds must an oxygen atom make to be stable?4. In order to break the bond holding the fructose and the glucose molecule together
you put back the atoms you took away. Why?5. What is the name given to the process breaking apart a molecule using a water
molecule?
The next page is a sheet of questions to copy for the kits.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
The white shape represents a glucose molecule with a formula of C6H12O6.
The maroon shape represents a fructose molecule with a formula of C6H12O6 Both glucose and fructose are single sugars called monosaccharides.The pipe cleaner represents the covalent bonds.The white beads represent oxygen atoms. The black beads represent hydrogen atoms.Directions: Take a hydrogen atom off of one molecule and a hydrogen and oxygen atom off of the other molecule. Link the two shapes together through the remaining oxygen atom. Answer the following questions on a separate sheet of paper.
1. What small molecule can you make with the beads you took off each sugar molecule?2. The fructose and glucose have been linked together. They were monosaccharides.
Now they form what kind of saccharide?3. How many bonds must an oxygen atom make to be stable?4. In order to break the bond holding the fructose and the glucose molecule together you
put back the atoms you took away. Why?5. What is the name given to the process breaking apart a molecule using a water
molecule?
The white shape represents a glucose molecule with a formula of C6H12O6.
The maroon shape represents a fructose molecule with a formula of C6H12O6 Both glucose and fructose are single sugars called monosaccharides.The pipe cleaner represents the covalent bonds.The white beads represent oxygen atoms. The black beads represent hydrogen atoms.Directions: Take a hydrogen atom off of one molecule and a hydrogen and oxygen atom off of the other molecule. Link the two shapes together through the remaining oxygen atom. Answer the following questions on a separate sheet of paper.
1. What small molecule can you make with the beads you took off each sugar molecule?2. The fructose and glucose have been linked together. They were monosaccharides. Now
they form what kind of saccharide?3. How many bonds must an oxygen atom make to be stable?4. In order to break the bond holding the fructose and the glucose molecule together you
put back the atoms you took away. Why?5. What is the name given to the process breaking apart a molecule using a water
molecule?
The white shape represents a glucose molecule with a formula of C6H12O6.
The maroon shape represents a fructose molecule with a formula of C6H12O6 Both glucose and fructose are single sugars called monosaccharides.The pipe cleaner represents the covalent bonds.The white beads represent oxygen atoms. The black beads represent hydrogen atoms.Directions: Take a hydrogen atom off of one molecule and a hydrogen and oxygen atom off of the other molecule. Link the two shapes together through the remaining oxygen atom. Answer the following questions on a separate sheet of paper.
1. What small molecule can you make with the beads you took off each sugar molecule?2. The fructose and glucose have been linked together. They were monosaccharides. Now
they form what kind of saccharide?3. How many bonds must an oxygen atom make to be stable?4. In order to break the bond holding the fructose and the glucose molecule together you
put back the atoms you took away. Why?5. What is the name given to the process breaking apart a molecule using a water
molecule?
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Teacher NotesTraining Lab – Identifying Organic Compounds in Foods
Equipment to Prepare:
1. 6 – 13 mm x 100mm test tubes placed in a 250ml beaker/group2. Some method for students to label their six test tubes – transparent tape and waterproof
pen works well and then can be soaked off in warm, soapy water later.3. 1 50o C hot water bath/group (hot plates with 250 mL beakers of water work well)4. 18 small beakers or containers (3 for each of the 6 organic food solutions students will be
testing). Place these in a central location and have the students come to central location to use them.
5. 18 labeled eye droppers or disposable pipettes (3 for each of the 6 organic food solutions)6. 3 or 4 Biuret Test Stations (dropper bottles are the easiest to use).7. 3 or 4 Benedict’s Test stations (dropper bottles are the easiest to use – the number of
water baths dictates the number of Benedict’s Test Stations.8. 3 or 4 Lugol’s Test Stations (Lugol’s is light sensitive and is best in a brown bottle with a
dropper but you can put some solution in a small beaker or Erlenmeyer flask with a disposable pipette or eye dropper)
Solutions to Prepare:
8. Biuret, Benedict’s, and Lugol’s solutions are poured from their shipping bottles without dilution.
9. Distilled Water Food Solution – tap water works fine10. Sucrose Food solutions – sucrose doesn’t react with any of the test solutions so it’s
easiest to cheat and use plain water for the sucrose solution. Otherwise, add one heaping teaspoon of table sugar to 500 mL of water.
11. Concentrated Glucose Food Solution – add 40 mL of clear corn syrup to 500mL of water12. Dilute Glucose Food Solution – add 10 mL of the Concentrated Glucose Solution above
to 500 mL of water13. Starch Food Solution – use a can of laundry spray starch from the store and spray for 15
seconds into a beaker filled with 500 mL of water OR put a teaspoon of cornstarch into 500 mL of water and mix well.
14. Protein Food Solution – stir .5 or ½ of a heaping teaspoon of powdered albumin into 500 mL of water
500 mL of each organic food solution should be more than enough for 5 classes.
Comments/Problems
This lab should take 2 days to complete including answering the questions. The usual delay is at the Benedict’s Test because it must heat for 5 minutes. Often the color change takes place before the 5 minutes are up but students like to be accurate with their protocol. Timers are handy but not necessary. Some students may need to be given the hint to look for color changes. The color changes should be obvious to students once they understand what to look for. It is helpful to have them hold a white piece of paper behind the test tubes in order to see the Biuret Test turn a solution light purple.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
TRAINING LAB – IDENTIFYING ORGANIC MOLECULES IN FOODS
BACKGROUND: You now know how important lipids, proteins, and carbohydrates are in your diet and how your body uses these molecules once you eat them. Is there a way to tell if these nutrients are actually present in the foods we eat? YES!! There are several commonly used tests for organic molecules.
SAFETY PRECAUTIONS!!!! WEAR YOUR GOGGLES AT ALL TIMES!! Be careful with solutions – wash your hands thoroughly after we are finished. Lugol’s Iodine Solution will stain your clothing. Report any broken glass immediately!!!!
PROCEDURES:
1. Completing the following tests:-Biuret Test-Lugol’s Iodine Test-Benedict’s Test
2. Complete the above tests on the following food molecules:-Distilled water -Sucrose solution
-Dilute glucose solution -Starch solution
-Concentrated glucose solution -Protein solution
3. EACH PERSON SHOULD RECORD THE DATA COLLECTED FROM THIS TRAINING LAB IN THEIR OWN TABLE.
4. Get six test tubes in a beaker. Label your tubes with the 6 food molecule solutions to be tested using clear tape and a ball point pen, pencil or Sharpie® pen.
5. THE BIURET TESTa. Add 20 drops of each food molecule solution to their respective test tubesb. Add 3 drops of Biuret Test solution to each test tube.c. Observe and record the results in your table (look for a color change)d. Rinse out your test tubes
6. THE LUGOL’S IODINE TESTa. Add 20 drops of each food solution to their respective test tubesb. Add 1 or 2 drops of Lugol’s Iodine Test Solution to each tubec. Observe and record the results in your table (look for a color change)d. Rinse out your test tubes.
7. THE BENEDICT’S TESTa. Add 20 drops of each food molecule solution to their respective test tubesb. Add 10 drops of Benedict’s Test Solution to each tube.c. Place tubes in a hot water bath for 5 minutesd. Immediately observe the results and record your observations in your data table.e. CLEAN UP!!!! Remove any labels from your test tubes, rinse them out and
then wash them in the soapy water using the test tube brush. Rinse again and turn upside down in the container to dry.
G & C Ulmer
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
My lab partner’s name is _________________________________.
Result Table:
Distilled
H2O
Dilute
Glucose
Solution
Conc.
Glucose
Solution
Sucrose
Solution
Starch
Solution
Protein
Solution
Biuret
Benedict’s
Lugol’s
Notes:
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
QUESTIONS: Identifying Organic Compounds in Foods
1. Carefully fill in the table with observational data. This table will be used to determine what molecules are present in an unknown solution.
2. What solution was included in this lab as a CONTROL to help you interpret your results? _________________________
3. Which test would you use if you wanted to detect the presence of a protein in an unknown solution? __________________________How could you determine if a protein was present?
4. Which test would you use if you wanted to detect the presence of a starch in an unknown solution? ______________________. How would you know if starch was present?
5. Which test would you use if you wanted to detect the presence of glucose in an unknown solution? _____________________ How would you tell if glucose was present?
6. You are asked to analyze a food substance to determine what organic food molecules are present. You observe a positive reaction with Benedict’s and Biuret solutions. What can you conclude about this food? (A positive reaction means that there was a color change.)
7. Do you have any way of telling how concentrated the glucose is in a food sample? _____ if yes – explain how, if you answered no, draw a picture of your favorite food.
8. Do you have any way of determining if sucrose is present in a food sample? ________ Explain your answer.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
A Taste of Trouble: McMush Lab
Just what is in a fast food meal??? We hear that fast food meals are "bad", but do they have anything good in them? Can they provide some nutrition? Are they really high in fat? Well, let's
find out!!!
Carbohydrates, proteins, fats, vitamins, and other nutrients provide your body with energy and provide the raw materials necessary to carry on life activities. These compounds are present in the plants and animals you use as food. In this lab, you will test for specific compounds and then determine if these are in a fast food value meal in sufficient quantities.
Materials:
McDonald's value mealBenedict's solution- tests for sugar (carbohydrates)Biuret solution- tests for proteinLugol's iodine solution- tests for starch (complex carbohydrates)Silver NitrateBrown paper (craft paper or a brown paper bag)Blender- it must be mixed together to test it!!
SAFETY NOTE!!! FOR THIS LAB, YOU MUST WEAR EYE PROTECTION!!!
To determine if these substances are in a sample of McMush, it is important to know what a positive test looks like for each of these components. So, the first part of the experiment will be to run tests on known samples to discover what a positive test looks like. If you have already done the Training Lab for Macromolecule ID just do the lipid and sodium test before going on to Part II.
Part I: Testing of Known Substances
Protein test: 1. Place 5 ml of the protein solution (this is actually ground up egg white, which is pure protein)
into a test tube.2. Add ten drops of Biuret solution.3 Observe any color change. Record it in chart.
Glucose test (simple sugar):
1. Place 5 ml of the glucose solution into your test tube.2. Add 3 ml of Benedict's solution. Place the tube in a beaker of boiling water and boil for five
minutes. Use test tube clamps to hold hot test tubes.3. Observe any color change. Record it in chart.
Starch test (complex carbohydrate):
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
1. Place 5 ml of the starch solution into a test tube. 2. Add 5 drops of Lugol's iodine solution. 3. Observe any color change. Record it in chart.
Lipid Test
1. Place 1 ml of vegetable oil on one small area of a paper bag. On another area, place a small amount of butter.
2. After a few seconds, remove the oil and butter with a paper towel. 3. Foods that contain lipids will leave a translucent mark on brown paper bag material. Record
results in the chart.
Sodium Test
1. Place 2 ml of salt solution in a test tube.2. Add 1 ml of distilled water (for a 3 ml total of solution)3. Transfer 2-3 drops of silver nitrate solution to a test tube.4. A cloudy solution indicates the presence of sodium; a precipitate may form.
Record your results in a data table:
Food Substance Reagent test Positive Results
Protein Biuret solution
Sugar Benedict's solution
Starch Lugol's iodine solution
Lipid Brown Bag
Sodium Silver nitrate
NOTES and Observations:
Modified from J. Brown
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Part II: McMush!
The McDonalds Value Meal will be blended and for a sample of "filtered McMush" to work with.
1. Repeat the reagent tests above using 5 ml of the McMush solution for each test (except for the lipid test, where only 1 ml of filtered McMush is needed, and the sodium test, using 2 ml of filtered McMush solution).
2. Describe and record results in the following table.
Food Substance Reagent test Test Results
Protein Biuret solution
Sugar Benedict's solution
Starch Lugol's iodine solution
Lipid Brown Bag
Sodium Silver nitrate
Total Fat Boil/Cool
PART III: McMush - Demonstration of Fat Content. This could be done as a demonstration.
Materials:
McMush 1 hot plate 1 - 500 ml beaker 1 - 200 ml graduated cylinder
100 ml of water 2 oven mitts 1 wooden spoon 1 refrigerator to cool McMush mixture
Procedure Overview:
1. The complete meal will be blended to make the McMush.2. A large sample of the meal will be heated.3. From the heated sample, 100 ml will be taken to be cooled.4. Results from the 100 ml sample are representational of the complete meal.
Procedure: 1. Preheat the hot plate.2. Break up meal into small pieces and blend.3. Pour part of the blended McMush into a 500 ml beaker.4. Add 100 ml of water to the McMush and stir well.5. Boil McMush mixture gently for 15 minutes.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
6. Use oven mitts to protect your hands and pour the warm McMush mixture into a graduated cylinder. Then cool in the refrigerator for 15 minutes. This can stay in the refrigerator overnight if necessary but cover it with aluminum foil or plastic wrap.
7. Remove McMush from the refrigerator and measure the amount of accumulated fat at the top of the graduated cylinder.
8. Record results.9. Dispose of mixture according to the teacher’s directions.10. Clean glassware with warm soapy water.
Expected Outcome:
The fat will form a layer at the top and solidify as it cools. Calculate the percent of fat in the McMush meal by dividing the ml of fat by the total mL of the sample. For example, if there is 40 mL of fat out of a total of 100 ml of sample. This would indicate the total meal contained 40% fat.
Conclusion:
Write the results to this lab as a letter to Katrina telling her the results of the test and what your recommendations would be about eating A Happy Meal at McDonalds.
How do you think a Happy Meal compares to other fast food meals? How would you go about testing your hypothesis? Describe how you would design an experiment to compare other foods to the McMush experiment.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
A Taste for Trouble Part V: Katrina was actually the first one in the room that morning. Her science teacher looked
up from the paper she was reading and smiled. “Good morning Katrina! I was just reading the
essay you wrote about the video “SuperSize Me!” You really have some strong feelings about
fast food!”
Katrina grinned, “Well, it has been a love/hate relationship. I am happy to say I have
kicked the habit. It was very emotional watching the video knowing I had done so much harm to
my body by eating too much fast food. I never realized I was in the grip of an addiction.
Knowledge is power, as my doctor is fond of saying!”
“It sure is! My job is all about giving you guys lots of power! She smiled as she went to
the back of the classroom.
Jeri drooped into a chair. Her eyes lit up when she saw Katrina already in the room.
“Ah! BFF you wouldn’t have a spare candy bar on you would you? I didn’t get a chance to eat
breakfast this morning and I am feeling really shaky.”
Katrina reached into her enormous bag and pulled out a banana, “Your in luck, Jeri, I
have something even better!”
Jeri sighed as she gratefully took the banana. “Yeah, your right but I do miss the big bag
full of candy sometimes.”
“I still think you need to go see Dr. Carter. He told me sometimes people have low
blood sugar and it can lead to diabetes too. Not a fun disease to have. I am determined to lose
enough weight so maybe I can stop taking insulin.
“You’re probably right,” sighed Jeri. “I should learn by your example. I’ll talk to my mom
about getting an appointment. Hey, I wonder if there are other diseases caused by nutrition.
Maybe that is what we can do for our biology research project.” She moved her book bag out of
the way as students began pouring into the classroom.
“I’ll bet there are. It seems you really are what you eat. I was watching Discovery
channel last night and they said more people are suffering from diabetes in other countries too.
It is becoming an epidemic everywhere. I was thinking our research project could be about how
nutrition is changing in developing countries and causing an increase in diabetes in countries
where it wasn’t a problem before. How sad! My old beloved fast food may be causing disease
across the world!”
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
“That sounds like a great idea, Katrina,” their science teacher remarked as she returned
to the front of the room. “Let’s get started!”
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
A Taste for Trouble Culminating Project You must:
1. Research the effects of diabetes on the body.
2. Find and give a brief summary of 2 other diseases whose predominant cause is diet and lifestyle choices.
3. Find information about global increases in these diseases over the past 20 years.
4. Turn in your bibliography and references when you present your project.
Choose a format to present your findings:
1. Video news report
2. Pamphlet
3. Radio Spot or podcast
4. Poster
5. Story book
6. Other (make sure you get teacher approval)
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
ReferencesBrown, Judy; The American Biology Teacher, Vol. 56, NO 8 November/December 1994
Campbell, Neal A., Reece, Jane B., Taylor Martha R., Simon, Eric J., Biology Concepts & Connections, 5th Edition 2006 Pearson Education, inc.
Ulmer, Greg and Ulmer, Carolyn; ABC Technologies, copyright 2000
Nutritional Calculator: http://www.bmi-calculator.net/bmr-calculator/bmr-formula.php
Inches to centimeters calculator: http://manuelsweb.com/in_cm.htm
BMI Calculator: http://www.nhlbisupport.com/bmi/
Assessing your risk: http://www.nhlbi.nih.gov/health/public/heart/obesity/lose_wt/risk.htm
National Heart, Lung and Blood Institute Obesity Initiative Slide Show Sets: http://hp2010.nhlbihin.net/oei_ss/menu.htm#sl2
National Heart, Lung and Blood Institute Obesity Initiative Menu Planner: http://hp2010.nhlbihin.net/menuplanner/menu.cgi
NutritionData.com, 1166 Sixth Avenue, New York, NY 10036 USA: http://www.nutritiondata.com/
U.S. Department of Health and Human Services, U.S. Food and Drug Administration (FDA), How to Understand and Use the Nutrition Facts Label: http://www.fda.gov/Food/LabelingNutrition/ConsumerInformation/UCM078889.htm
Calorie King, Calorie Counter: http://www.calorieking.com/foods/?partner=healthtrek
Hemi Weingarten, Fooducate Blog, Copyright © 2008-2010 Fooducate: http://www.fooducate.com/blog/2008/10/25/1862-2008-a-brief-history-of-food-and-nutrition-labeling/
Group Health Cooperative Foundation, HealthTrek.org: http://www.healthtrek.org/lesson01.php
Mayo Foundation for Medical Education and Research, Interactive nutrition label: http://www.mayoclinic.com/health/nutrition-facts/nu00293
Lab Tests Online, A public resource on clinical lab testing from the professionals who do the lab testing. http://www.labtestsonline.org/understanding/analytes/glucose/test.html
Lab Tests Online, http://www.labtestsonline.org/inc/reports/SampleReport.html
WikiHow: Lab report example http://www.wikihow.com/Read-and-Understand-Medical- Laboratory-Results
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
"Super Size Me." Internet Movie Data Base. 3 Sep 2006 http://www.imdb.com/title/tt0390521/plotsummary
http://www.lessonplanspage.com/PEODoSomethingAboutEatingHealthyUnitDay5SuperSizeMe912.htm
USDA National Nutrient Database for Standard Reference: http://www.nal.usda.gov/fnic/foodcomp/search/index.html
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
A Taste for Trouble Answer SheetsQuestion for a Taste for Trouble Part I
From the story, generally describe the body type of each girl:
Katrina: Overweight/Obese, out of shape, has difficulty walking quickly
Jeri: thin, hungry to the point of passing out
What do you know about Katrina’s food preferences from the story? She keeps a stash of candy bars in her bag so she likes sweets and junk food, and misses her variety of fast food from the big city
What physical symptoms does Katrina have (think about what is making her uncomfortable in the scene)? She is thirsty and needs to pee, is out of breath and tired without much physical exertion. The school is not really THAT big in such a small town.
What physical symptoms does Jeri have? Jeri says she is about to pass out from hunger – she could just be a drama queen or really feel faint.
What do you know about Jeri’s eating habits from the story? She hasn’t eaten yet and she will eat candy
Internet Research: For girl and each symptom, make a list of possible causes.
Katrina: May include the following
Clue #1: Frequent need to pee
Clue #2: Frequent Thirst Answers may vary
Clue #3 : Out of breath
Clue #4: Over-weight
Urinary tract infection
Eating salty or sugary food asthma Over-eating
Diabetes Diabetes Congestive Heart failure
Lack of exercise
Pregnancy Chronic kidney failure COPD chronic obstructive pulmonary disease
hypothyroidism
Diuretics Potassium deficiency pneumonia Eating disorder
Overactive bladder
Hyperparathyroidism Out of shape Sedentary lifestyle
Drugs like Ecstasy Heart attack
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
What questions would you ask Katrina to help you narrow down her possible health issues?Answers will vary but might include: How active are you? Does it hurt when you urinate?
Are you pregnant? Do you play a sport? Are you taking any medication? About how many calories do you eat in a day? Have you been sick recently? Do you have asthma? Have you seen a doctor recently? Do you take recreational drugs? Do you drink a lot of caffeinated drinks? What is her height and weight?If you were her doctor what type of tests would you request in order to diagnosis Katrina? Answers will vary but might include: Glucose blood test, Thyroid function tests
Jeri
Clue #1: very thin Clue#2: Feels like passing out from hunger
High metabolism Amphetamine abuse
Anorexia Diabetic hypoglycemia
Poor diet
Poor absorption of nutrients
What questions would you ask Jeri to help you narrow down her possible health issues? Answers will vary but might include: How often do you eat? Have you ever thrown up after eating? Do you take drugs?
If you were her doctor what type of tests would you request in order to diagnosis Jeri? Answers will vary but might include: Blood glucose, Thyroid hormone levels
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Reading Nutrition Labels KEY
Use the nutrition label to answer the following questions.
What food item does this nutrition label describe? __________Crackers ________________ 5. What is the serving size? ___5 Crackers___. How
many servings are in the container? ____28_____
6. How many Calories are in each serving? 80 Calories. How many Calories are from fat? 40 Calories
7. a. Total fat = ___4.5___ g Saturated Fat = ____1___ g Trans fat =__0__ g Cholesterol = _0__ g Sodium = ____140___ mg
b. Total Carbohydrates = ___9__g Dietary Fiber = ___>1__g Sugars = ______9____g Proteins =____1_g
4. Vit. A __0_% Vit. C __0_% Calcium _0_% Iron _2_%
5. Of all the ingredients in % DV listed on the label this food contains the most ___Fat__
6. In terms of the % Daily Values of needed nutrients would this food be considered Low (5% or less) or High (20% or more) ____Low____ Explain Most of the nutrients are 5% or less. Those that are higher than 5% or only at 6 or 7% with some much lower than 5%. As a whole it would average out to be at or below 5%
7. The Percent Daily Values (%DV) are based upon a 2,000Calorie Diet. What % DV does this food item supply of dietary fiber? 1 % How many grams of Dietary Fiber are required for a person needing 2,000 Cal/day 25 g and by a person needing 2,500 Cal/day 30 g.If you needed 3,000 Cal/day what amount of dietary fiber should you have in your diet? 35 g.
Some amounts of certain nutrients go up with Calorie requirements. Which nutrient amounts stay the same regardless of Calorie needs? Cholesterol and Sodium
Different macromolecules are bonded together differently and have differing amounts of calories. Generally Lipids (fats) are good energy storage molecules because they have about 9 Cal/g, both Carbohydrates and Proteins have about 4 Cal/g. Complete the following table:
Beef Stew Soup 170 Cal/cup Protein 10g = 40CalCarbohydrates 19g = 76Cal
Total fat 5g = 45 CalTotal Sodium 810 mg
Vegetable Beef Soup 120 Cal/cup
Protein 7g = ___28__CalCarbohydrates 19g = __76_Cal
Total fat 2g = _18_CalTotal Sodium 410 mg
Light Beef and Barley Soup 80 Cal/cup
Protein 5 g = 20 CalCarbohydrates 14g = _56__Cal
Total fat 1.5g = 13.5CalTotal Sodium 410 mg
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Nutrition Label Comparison Activity KeyGlue or tape three of your nutrition labels below. If the name of the food is not on the label, write it in the blanks labeled Food: Answers will vary based on individual Nutrition LabelsFood A: Food B: Food C:
1. Which of these foods has the most Calories per serving? ______ answers will vary
2. Which of these foods has the least amount of total fat? _______ answers will vary
3. Which of these foods would be considered high in % Daily Value? _ answers will vary
4. Which of these foods has the most sodium? ____ answers will vary
5. Which of these foods would be the most nutritious? ____ answers will vary
6. What did you choose this food as most nutritious? answers will vary. Hopefully the students will consider fat and sodium content. This question is enlightening as to what students think of as “nutritious”
7. Which food has the least saturated fat? answers will vary
8. Which food has the most dietary fiber? answers will vary
9. Which food would be the best choice if you had to limit your carbohydrates? answers will vary
10. Which of these foods would be the best if you had to limit your sodium intake? answers will vary
The assignment to bring 6 nutrition labels should give each student enough labels to choose 3 with enough information to evaluate. Some nutrition labels have 0% of a lot of the nutrients. Those labels are not instructive in this activity. It helps to have a stockpile of labels in case students have brought mostly junk food labels or do not have enough to do the activity.
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Katrina’s Homework Answer KeyKatrina must find her height and weight. Luckily they have an accurate bathroom scale she can use at home. Her mom helped her find her height by using a ruler, a pencil and a tape measure. Katrina stood straight with her back against the wall and her mom put the ruler on top of her head at a right angle to the wall. She made a small mark with the pencil which could easily be erased later. They used the tape measure to measure from the floor to the mark. Katrina is 5’5” tall and weighs 295 pounds. She is 16 years old. Nicole wanted her to use one of the following formulas:
English Basal Metabolic Rate (BMR) FormulaWomen: BMR = 655 + ( 4.35 x weight in pounds ) + ( 4.7 x height in inches ) - ( 4.7 x age in years )Men: BMR = 66 + ( 6.23 x weight in pounds ) + ( 12.7 x height in inches ) - ( 6.8 x age in year )OrMetric Basal Metabolic Rate (BMR) FormulaWomen: BMR = 655 + ( 9.6 x weight in kilos ) + ( 1.8 x height in cm ) - ( 4.7 x age in years )Men: BMR = 66 + ( 13.7 x weight in kilos ) + ( 5 x height in cm ) - ( 6.8 x age in years )
Calories are needed to chew and digest your food. This is referred to as Specific Dynamic Action or SDA. It is figured as 10% of your Basal Metabolic Rate (BMR) SDA = BMR x .1
Specific Dynamic ActionSDA BMR x .1
To determine your total daily calorie needs, multiply your BMR by the appropriate activity factor, as follows:
Activity Factor Calorie-CalculationIf you are sedentary (little or no exercise) BMR x 1.2If you are lightly active (light exercise/sports 1-3 days/week) BMR x 1.375If you are moderately active (moderate exercise/sports 3-5 days/week) BMR x 1.55If you are very active (hard exercise/sports 6-7 days a week) BMR x 1.725If you are extra active (very hard exercise/sports & physical job or 2x training) BMR x 1.9
Katrina’s BMR = 655 + (4.35 x 295 lbs.) + (4.7 x 65”) – (4.7 x 16 years old)
655 + 1283.25 + 305.5 – 75.2 = 1513.55
Katrina’s SDA = BMR x .1 1513.55 x .1 =151.355
Katrina’s Activity Calories = BMR x 1.2 1513.55 x 1.2 = 1816.26 Cal/day
Katrina’s Results (round the Total to the nearest whole number)
Katrina’s BMR 1513.55Katrina’s SDA 151.355
Katrina’s Activity Calories 1816.26Total # of Calories/Day 3,481.165
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Find Your Calorie Needs Answer Key
Use the same information Katrina used in her homework to calculate your BMR, SDA and Activity Calories. Answers will vary with each student’s height and weight. Some students are sensitive about personal information. Tell them this assignment is in confidence and they do not have to share their answers with anyone except you so you can check their work.
Your Height ___ft. ____in. = _____in x 2.54 = ______ cm Your Weight lbs. Kg
Convert feet into inches by multiplying by 12.English Basal Metabolic Rate (BMR) FormulaWomen: BMR = 655 + ( 4.35 x weight in pounds ) + ( 4.7 x height in inches ) - ( 4.7 x age in years )Men: BMR = 66 + ( 6.23 x weight in pounds ) + ( 12.7 x height in inches ) - ( 6.8 x age in year )OrMetric Basal Metabolic Rate (BMR) FormulaWomen: BMR = 655 + ( 9.6 x weight in kilos ) + ( 1.8 x height in cm ) - ( 4.7 x age in years )Men: BMR = 66 + ( 13.7 x weight in kilos ) + ( 5 x height in cm ) - ( 6.8 x age in years )
Calories are needed to chew and digest your food. This is referred to as Specific Dynamic Action or SDA. It is figured as 10% of your Basal Metabolic Rate (BMR) SDA = BMR x .1
Specific Dynamic ActionSDA BMR x .1
To determine your total daily calorie needs, multiply your BMR by the appropriate activity factor, as follows:
Activity Factor Calorie-CalculationIf you are sedentary (little or no exercise) BMR x 1.2If you are lightly active (light exercise/sports 1-3 days/week) BMR x 1.375If you are moderately active (moderate exercise/sports 3-5 days/week) BMR x 1.55If you are very active (hard exercise/sports 6-7 days a week) BMR x 1.725If you are extra active (very hard exercise/sports & physical job or 2x training) BMR x 1.9
Your BMR = 655 + (4.35 x __________lbs.) + (4.7 x __________”) – (4.7 x _____years old)
655 + ________+ ________ - __________ = __________
Your SDA = BMR x .1 __________x .1 = ____________
Your Activity Calories = BMR x 1.2 __________x 1.2 = _____________Cal/day
Your Results (round the Total to the nearest whole number)
Your BMRYour SDA
Your Activity CaloriesTotal # of Calories/Day
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Nicole also asked Katrina to keep track of the food she ate each day. She specifically asked her to also track the beverages she consumed, including water. Use the following to keep your own food diary. The food and amounts will vary. The example below is Day 1 from Katrina’s Diary
Daily Food Diary (KEY)Food Day 1 Calories Day 2 Calories Day 3 CaloriesBreakfast 4 Choc. Poptarts 420
Choc. InstantBreakfast/milk 220
Snack 2 oz. Snicker bar 2712 pkgsCheese Crackers 38012 oz Gatorade 310
Lunch Big Mac 576Supersize Fries 61042 oz Dr. Pepper 380Mc Donaldlandcookies 260
Snack Skittles 250Pkg Cheese puffs 23020 oz water 0
Dinner Stoufer’s Lasagna 690Dinner salad 90Ranch Fatfreesalad dressing 482 Garlic bread 200
Snack Bag Kettle corn 1806 slice/bake Choc. Chip cookies 45010 oz 2% milk 160
Total Calories 5,725Calculated Required Cal/Day 3,481Difference +2,244
Subtract the actual Calories you ate from your Calculated Required Cal/Day. If you ate more put a + sign in front of the number, if you ate less put a – sign.
Advanced Diary: Students could also keep track of carbohydrates, proteins and fats and determine how many grams of each they eat each day.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
My lab partner’s name is _________________________________.
Result Table: KEY
Distilled
H2O
Dilute
Glucose
Solution
Conc.
Glucose
Solution
Sucrose
Solution
Starch
Solution
Protein
Solution
BiuretBlue Blue Blue Blue Blue
Light
Purple
Benedict’sOrange Orange Orange Orange
Purple
BlackOrange
Lugol’sBlue Blue Orange
Light
OrangeBlue Blue
Notes:
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
QUESTIONS: Identifying Organic Compounds in Foods Answer Key
1. Carefully fill in the table with observational data. This table will be used to determine what molecules are present in an unknown solution.
2. What solution was included in this lab as a CONTROL to help you interpret your results? ______DISTILLED WATER_______
3. Which test would you use if you wanted to detect the presence of a protein in an unknown solution? ____BIURET TEST________How could you determine if a protein was present?
THE FOOD SOLUTION WOULD TURN LIGHT PURPLE
4. Which test would you use to detect the presence of a starch in an unknown solution? ___LUGOL’S IODINE TEST______. How would you determine if starch was present?
THE FOOD SOLUTION WOULD TURN PURPLE/BLACK
5. Which test would you use to detect the presence of glucose in an unknown solution? __BENEDICT’S TEST__________ How would you determine if glucose was present?
THE FOOD SOLUTION WOULD TURN ORANGE
6. You are asked to analyze a food substance to determine what organic food molecules are present. You observe a positive reaction with Benedict’s and Biuret’s solutions. What can you conclude about this food? (A positive reaction means that there was a color change.) THE FOOD MUST CONTAIN BOTH PROTEIN AND GLUCOSE
7. Do you have any way of telling how concentrated the glucose is in a food sample? YES if yes – explain how, if you answered no, draw a picture of your favorite food.THE MORE CONCENTRATED THE GLUCOSE SOLUTION, THE DARKER ORANGE THE FOOD SAMPE WILL APPEAR AFTER TESTING WITH BENEDICT’S
9. Do you have any way of telling if sucrose is present in a food sample? NO Explain your answer.
SUCROSE DID NOT HAVE A POSITIVE REACTION (COLOR CHANGE) WITH ANY OF THE TESTS WE PREFORMED
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Macromolecule 101 Answer KeyBonding activity with plastic shapes, pipe cleaners and pony beads to model hydrolysis and
dehydration synthesis.
This activity is done with plastic needlepoint canvas shapes, pipe cleaners and pony beads. Kits of these materials, along with a direction sheet, are made up and put into snack-size zip top bags and keep in a gallon size bag to be used over and over again. This hands-on activity helps students understand dehydration synthesis and hydrolysis.
Pieces of the kit
The white shape represents a glucose molecule with a formula of C6H12O6.
The maroon shape represents a fructose molecule with a formula of C6H12O6 Both glucose and fructose are single sugars called monosaccharides.The pipe cleaner represents the covalent bonds.The white beads represent oxygen atoms. The black beads represent hydrogen atoms.Directions: Take a hydrogen atom off of one molecule and a hydrogen and oxygen atom off of the other molecule. Link the two shapes together through the remaining oxygen atom. Answer the following questions on a separate sheet of paper.
1. What small molecule can you make with the beads you took off each sugar molecule?
2. The fructose and glucose have been linked together. They were monosaccharides. Now they form what kind of saccharide?
3. How many bonds must an oxygen atom make to be stable?4. In order to break the bond holding the fructose and the glucose molecule together
you put back the atoms you took away. Why?5. What is the name given to the process breaking apart a molecule using a water
molecule?
6. What small molecule can you make with the beads you took off each sugar molecule? Water (H2O)
7. The fructose and glucose have been linked together. They were monosaccharides. Now they form what kind of saccharide? They form a disaccharide
8. How many bonds must an oxygen atom make to be stable? Two – Its atomic number is 8 so it has two electrons in the first energy level and six in the next. It needs to have to more electrons in order to be stable.
9. In order to break the bond holding the fructose and the glucose molecule together you put back the atoms you took away. Why? Oxygen MUST have two bonds in order to break the covalent bond holding the fructose and glucose together one hydrogen from the water bonds to the oxygen which is bond to the carbon of one sugar and the hydroxyl (-OH) bonds to the carbon on the other sugar.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
10. What is the name given to the process breaking apart a molecule using a water molecule? Hydrolysis
A Taste of Trouble: McMush Lab Answer Key
Part I:
Food Substance Reagent test Positive Results
Protein Biuret solution Light Purple
Glucose Benedict's solution Orange
Starch Lugol's iodine solution Purple/Black
Lipid Brown Bag translucent
Sodium Silver nitrate cloudy
Part II:
Food Substance Reagent test Test Results
Protein Biuret solution Light purple
Glucose Benedict's solution orange
Starch Lugol's iodine solution Purple/Black
Lipid Brown Bag translucent
Sodium Silver nitrate cloudy
Total Fat Boil/Cool
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
A Taste for Trouble Student Manual
A Case Study of Nutritional Health as an introduction to Macromolecules for High School Biology Classes
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
A Taste for Trouble: Part I
Katrina huffed down the hall. She was pooped! It was just second period but it had already been a long day finding her way around the unfamiliar high school. It was bad enough to move to a new town right before her sophomore year but it seemed all her classes were as far apart as humanly possible. She found room 128 and squeezed her large frame into the first available seat. She would rather have sat at the back where she might escape notice, but being in front she might actually make a badly needed restroom break before she made her way across this ridiculous building to her Spanish class.
A skinny blonde slipped into the chair next to her. “I am so hungry I am about to pass out!” She exclaimed. “You wouldn’t have anything to eat in that big bag of yours would you?” She eyed Katrina’s enormous purple purse.
“I do keep a stash of candy bars,” Katrina confessed. “I’m usually hungry myself.” She rummaged in her bag and handed the girl a chocolate candy bar. “Not to mention thirsty! I am Katrina by the way.”
“Jeri” mumbled the girl through a mouthful of candy, “nice to meet you.” She swallowed, “Thanks for saving me. I heard they have vending machines in the building but I am still trying to find my classes and I know I will never see my locker again. Why does everything have to look the same?”
“I know,” sighed Katrina. “If they ever take down that gruesome science poster at the front of this hall I will never find my way back to Kansas!”
Jeri did a double take as she took another bite of candy, “Gosh! Are you from Kansas too?”
“No, I am from Missouri, I was referring to the Wizard of Oz, not that I am Dorothy or anything but moving from St. Louis to this little town out in the sticks is like being plunged into the land of the munchkins. No offense, but I prefer a larger town where they have more fast food choices and shopping malls. Are you really from Kansas?”
Several students careened into the room seconds after the tardy bell disgustedly looking at the remaining seats.
“OK, class! Let’s get started!” The teacher’s voice startled the class into attention. “Those who have just come in don’t worry about hunting for the perfect spot. I am going to assign seats for the first few weeks anyway.” Audible groans from the teens. “Also, I won’t be counting you tardy today but I will tomorrow so get here on time! It also looks like some folks have forgotten the “no food in class” rule from your handbooks! Remember to save your snacks for the nutrition break after 3rd period.”
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Jeri quickly slid the remaining candy bar down beside her on the seat. “Tell you later,” she mouthed to Katrina, who had crossed her legs and wished her last name was not so far down in the alphabet. She hoped this class would go by quickly! Her shot at making it to the small overcrowded girl’s restroom during passing time faded with the seating chart, never mind getting a much needed bottle of water. She would just have to be late next period. Perhaps her Spanish teacher would also be forgiving about tardiness on the first day of school.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Questions for a Taste for Trouble Part I
From the story, generally describe the body type of each girl:
Katrina: ______________________________________________________________________________
Jeri: ______________________________________________________________________________
What do you know about Katrina’s food preferences from the story? ______________________
______________________________________________________________________________
What physical symptoms does Katrina have (think about what is making her uncomfortable in the scene)? ____________________________________________________________________
______________________________________________________________________________
What physical symptoms does Jeri have? _____________________________________________
______________________________________________________________________________
What do you know about Jeri’s eating habits from the story? _____________________________
Internet Research: For girl and each symptom, make a list of possible causes.
Katrina:
Clue #1: Clue #2: Clue #3 : Clue #4:
What questions would you ask Katrina to help you narrow down her possible health issues?
If you were her doctor what type of tests would you request in order to diagnosis Katrina?
Jeri
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Clue #1: Clue#2:
What questions would you ask Jeri to help you narrow down her possible health issues?
If you were her doctor what type of tests would you request in order to diagnosis Jeri?
A Taste for Trouble: Part II
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
“I could wring her scrawny little neck!” exclaimed Katrina as she flopped down on the sofa, setting her Big Gulp cup on the ottoman in front of her while blowing her bangs out of her eyes. She let her heavy backpack slide to the floor off her shoulder as she struggled to untie her Nike’s and untangle her purse all at the same time. “She has no idea what it is like!” she ranted.
“What are you complaining about now!” asked her mother from the doorway. I am barely in the house before the griping begins. I understand how hard it is to switch schools when you are a teenager but give me a break already!” Moving hasn’t been a walk in the park for any of us.” Katrina rolled her eyes at her mom’s remarks.
Her mother changed her tone, hoping to prevent more drama. “Did you see Nicole today? She was so excited we were moving to town.”
“If you’re trying to change the subject, you just failed,” snorted Katrina. “She is the one whose scrawny neck I was eager to wring!”
“Oh! Katrina! That is not very nice. She was always your favorite cousin and you were happy she was at Central University!” returned her mother. “What was it you said? Her presence was the only saving grace in moving to this one-horse town or some such thing!”
“Was, is the operative word! When I met her in the Student Union, she had the gall to gasp and exclaim about how much weight I gained since last Christmas; AND then she went on and on about how she was going to get me into shape. Like she is so perfect! I could snap her in half like a twig! She went on and on about eating right and exercising until I thought I would barf! She expects me to go along with all of this. She may be skinny but she doesn’t know anything about manners! You don’t go around telling people they are fat; even if they are!”
“Well, honey, you know she is majoring in nutrition and fitness. It is only natural she would get excited about helping you.” replied Katrina’s mom.
“I don’t remember asking for help! But Miss Smarty Pants gave me homework to do! I am supposed to find my height and weight and use this stupid formula to figure out I am fat! I know I am fat! Good Grief! I am so tired I want to go to bed right now and I have 6 tons of stupid homework and plenty of it is math without fat formulas on top! Geez! And she wants me to meet her at the park so we can work out together. So yea, moving here looks like it is turning in to lots of walks in the park! I don’t have enough energy to do anything and she wants me to do more! That makes perfect sense! NOT!” She stood up and took a big swig of her soda as she headed down the hall to the bathroom.
“Well, dear” ventured her mom, “once you get a ton or two of the homework done, you can set the table for dinner. I got rid of some boxes so we can finally sit down and eat like an actual family tonight.”
“Great!” retorted, Katrina. “My last meal before I am doomed to count every blessed calorie for Miss Prissy Pants!”
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Reading Nutrition Labels
Use the nutrition label to answer the following questions.
What food item does this nutrition label describe? _________________________________________ 8. What is the serving size? __________. How many
servings are in the container? ____________
9. How many Calories are in each serving? _________. How many Calories are from fat? ___________
10. a. Total fat = _______g Saturated Fat = _________g Trans fat = __________g Cholesterol = __________g Sodium = __________mg
b. Total Carbohydrates = _____g Dietary Fiber = ______g Sugars = _______g Proteins =_________g
4. Vit. A ___% Vit. C ____% Calcium ____% Iron ____%
5. Of all the ingredients listed on the label this food contains the most _________________
6. In terms of the % Daily Values of needed nutrients would this food be considered Low (5% or less) or High (20% or more) ____________Explain_________________________________________________________________________________________________________________________________________________________________________________________________
7. The Percent Daily Values (%DV) are based upon a ____________Calorie Diet. What % DV does this food item supply of dietary fiber?__________% How many grams of Dietary Fiber are required for a person needing 2,000 Cal/day ________g and by a person needing 2,500 Cal/day ________g.If you needed 3,000 Cal/day what amount of dietary fiber should you have in your diet? _____g.
Some amounts of certain nutrients go up with Calorie requirements. Which nutrient amounts stay the same regardless of Calorie needs? _________________________________________Different macromolecules are bonded together differently and have differing amounts of calories. Generally Lipids (fats) are good energy storage molecules because they have about 9 Cal/g, both Carbohydrates and Proteins have about 4 Cal/g. Complete the following table:
Beef Stew Soup 170 Cal/cup Protein 10g = ________CalCarbohydrates 19g = _____Cal
Total fat 5g = ___________CalTotal Sodium 810 mg
Vegetable Beef Soup 120 Cal/cup
Protein 7g = ______CalCarbohydrates 19g = _____Cal
Total fat 2g = ___________CalTotal Sodium 410 mg
Light Beef and Barley Soup 80 Cal/cup
Protein 5 g = _________CalCarbohydrates 14g = _____Cal
Total fat 1.5g = __________CalTotal Sodium 410 mg
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Nutrition Label ComparisonGlue or tape three of your nutrition labels below. If the name of the food is not on the label, write it in the blanks labeled Food:Food A: Food B: Food C:
1. Which of these foods has the most Calories per serving? __________________________
2. Which of these foods has the least amount of total fat? __________________________
3. Which of these foods would be considered high in % Daily Value? __________________
4. Which of these foods has the most sodium? ___________________________________
5. Which of these foods would be the most nutritious? _____________________________
6. What did you choose this food as most nutritious? ______________________________________________________________________________________________________
7. Which food has the least saturated fat? _______________________________________
8. Which food has the most dietary fiber? _______________________________________
9. Which food would be the best choice if you had to limit your carbohydrates? _____________________________________________
10. Which of these foods would be the best if you had to limit your sodium intake? _____________________________________________
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Katrina’s Homework
Katrina must find her height and weight. Luckily they have an accurate bathroom scale she can use at home. Her mom helped her find her height by using a ruler, a pencil and a tape measure. Katrina stood straight with her back against the wall and her mom put the ruler on top of her head at a right angle to the wall. She made a small mark with the pencil which could easily be erased later. They used the tape measure to measure from the floor to the mark. Katrina is 5’5” tall and weighs 295 pounds. She is 16 years old. Nicole wanted her to use one of the following formulas:
English BMR FormulaWomen: BMR = 655 + ( 4.35 x weight in pounds ) + ( 4.7 x height in inches ) - ( 4.7 x age in years )Men: BMR = 66 + ( 6.23 x weight in pounds ) + ( 12.7 x height in inches ) - ( 6.8 x age in year )OrMetric BMR FormulaWomen: BMR = 655 + ( 9.6 x weight in kilos ) + ( 1.8 x height in cm ) - ( 4.7 x age in years )Men: BMR = 66 + ( 13.7 x weight in kilos ) + ( 5 x height in cm ) - ( 6.8 x age in years )
Calories are needed to chew and digest your food. This is referred to as Specific Dynamic Action or SDA. It is figured as 10% of your Basal Metabolic Rate (BMR) SDA = BMR x .1
Specific Dynamic ActionSDA BMR x .1
To determine your total daily calorie needs, multiply your BMR by the appropriate activity factor, as follows:
Activity Factor Calorie-CalculationIf you are sedentary (little or no exercise) BMR x 1.2If you are lightly active (light exercise/sports 1-3 days/week) BMR x 1.375If you are moderately active (moderate exercise/sports 3-5 days/week) BMR x 1.55If you are very active (hard exercise/sports 6-7 days a week) BMR x 1.725If you are extra active (very hard exercise/sports & physical job or 2x training) BMR x 1.9
Katrina’s BMR = 655 + (4.35 x ________lbs.) + (4.7 x ______”) – (4.7 x ______years old)
655 + ________+ ________ - __________ = __________
Katrina’s SDA = BMR x .1 __________x .1 = ____________
Katrina’s Activity Calories = BMR x 1.2 __________x 1.2 = _____________Cal/day
Katrina’s Results (round the Total to the nearest whole number)
Katrina’s BMRKatrina’s SDA
Katrina’s Activity CaloriesTotal # of Calories/Day
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Find Your Calorie Needs
Use the same information Katrina used in her homework to calculate your BMR, SDA and Activity Calories.
Your Height ___ft. ____in. = _____in x 2.54 = ______ cm Your Weight lbs. Kg
Convert feet into inches by multiplying by 12.English Basal Metabolic Rate (BMR) FormulaWomen: BMR = 655 + ( 4.35 x weight in pounds ) + ( 4.7 x height in inches ) - ( 4.7 x age in years )Men: BMR = 66 + ( 6.23 x weight in pounds ) + ( 12.7 x height in inches ) - ( 6.8 x age in year )OrMetric Basal Metabolic Rate (BMR) FormulaWomen: BMR = 655 + ( 9.6 x weight in kilos ) + ( 1.8 x height in cm ) - ( 4.7 x age in years )Men: BMR = 66 + ( 13.7 x weight in kilos ) + ( 5 x height in cm ) - ( 6.8 x age in years )
Calories are needed to chew and digest your food. This is referred to as Specific Dynamic Action or SDA. It is figured as 10% of your Basal Metabolic Rate (BMR) SDA = BMR x .1
Specific Dynamic ActionSDA BMR x .1
To determine your total daily calorie needs, multiply your BMR by the appropriate activity factor, as follows:
Activity Factor Calorie-CalculationIf you are sedentary (little or no exercise) BMR x 1.2If you are lightly active (light exercise/sports 1-3 days/week) BMR x 1.375If you are moderately active (moderate exercise/sports 3-5 days/week) BMR x 1.55If you are very active (hard exercise/sports 6-7 days a week) BMR x 1.725If you are extra active (very hard exercise/sports & physical job or 2x training) BMR x 1.9
Your BMR = 655 + (4.35 x _________lbs.) + (4.7 x __________”) – (4.7 x ______years old)
655 + ________+ ________ - __________ = __________
Your SDA = BMR x .1 __________x .1 = ____________
Your Activity Calories = BMR x 1.2 __________x 1.2 = _____________Cal/day
Your Results (round the Total to the nearest whole number)
Your BMRYour SDA
Your Activity CaloriesTotal # of Calories/Day
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Nicole also asked Katrina to keep track of the food she ate each day. She specifically asked her to also track the beverages she consumed, including water. Use the following to keep your own food diary.
Daily Food DiaryFood Day 1 Calories Day 2 Calories Day 3 CaloriesBreakfast
Snack
Lunch
Snack
Dinner
Snack
Total CaloriesCalculated Required Cal/DayDifference
Subtract the actual Calories you ate from your Calculated Required Cal/Day. If you ate more put a + sign in front of the number, if you ate less put a – sign in front of the number.
Advanced Diary: Students can also keep track of carbohydrates, proteins and fats and determine how many grams of each they eat each day.
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FOOD LABELS AND BIOLOGICAL MOLECULES
Alternate assignment sheet to track fat and protein along with carbohydrates
Make a list of all the food that you eat during a 3 day period. o If a food label is available use it to determine mass of carbohydrates, total lipids
(fats), and protein. o Information for foods such as meats and produce can be found on the internet
or in cookbooks. The following site is from the US Department of Agriculture which regulates labeling of foods.
http://www.nal.usda.gov/fnic/foodcomp/search/index.html
Go to the site, type in the name of the food, click “submit.” A list of foods matching the term will appear. Click on the one that is closest to
what was eaten. Scroll to the bottom of the page and click “submit.” Usually there is a couple of serving size choices. Unclick “100 grams” and select
what seems most appropriate, and click “submit.” Click “submit.” The next page contains a chart with more information than
needed. Refer to your chart to gather what is required for this assignment.
On a separate piece of paper, add each nutrient (total carbohydrate, protein, fat) and list the total grams in a chart.
Make a bar graph indicating the total grams of each nutrient. You will be graded on neatness and completeness, not on your dietary choices.
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Advanced food Diary:Food Diary
Food Approximate amount
Calories Total Carbs
(g)
Protein (g)
Total Fat (g)
Total Average per day
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
A Taste for Trouble Part III
“Katrina Martin” stated the nurse, in the brightly patterned scrubs, as she opened the door into the waiting room. “We are ready for you. Please come back with me and we’ll get started.” She smiled as she held the door open for Katrina. Her smile faded as she watched Katrina limp toward the door.
“Hi, Katrina, my name is Tracy. I will be helping Dr. Carter take care of you. I’ll put your stuff in the exam room; you just head toward the scales.” She nodded down the hall as she took Katrina’s purse and put it on a chair in a nearby exam room. She caught up with Katrina quickly and helped her step onto the medical scales. “This will just take a second and we will get you off that foot.” Tracy said in a business-like manner. “OK, let’s see.” She shifted the weights on the scale swiftly and wrote 290 lbs. in Katrina’s chart.
“Wow! I lost 5 pounds!” exclaimed Katrina. Tracy did a double-take as she had been expecting a groan. “Most people don’t get that excited about me taking their weight,” She explained.
“Well, that would usually describe me too except I just started to work on losing weight last week and am already making progress! I could really get into groaning about the remaining 290 pounds though if that would make you feel better!” exclaimed Katrina.
“No, no!” Tracy responded hastily. “I prefer my patients to be upbeat!” She pulled the height bar up and marked down 5’5” in the chart. “Let’s get you back to the room and get your pulse, blood pressure and temperature. But first, you get to pee in a cup!” She motioned Katrina toward the restroom door. “All the supplies are on the shelf with directions on this wall poster. Holler if you need me, I’ll be right here.”
Katrina emerged a few moments later presenting Tracy with the urine specimen as if it were a gift. “Voila, Madame!”
“I am glad the pain in your foot has not spoiled your sense of humor!” laughed, Tracy. “It is tough to be new in town but I bet you make friends easily.”
“I‘ve never really thought about it,” shrugged Katrina. “I’ve lived in St. Louis my entire life so I don’t know if I am good at making new friends or not. I sure miss my old ones though. Thank goodness for texting and Facebook!”
Back in the exam room Tracy quickly found Katrina’s vital signs and entered them into the chart. She and Tracy went over the pre-exam history Katrina had filled out in the waiting room. “I wish my mom could have come with me today,” sighed Katrina. “She is much better at all these details.”
“It would have been best if she was here but we will be getting your medical information from your doctor in St. Louis and we will be calling her to fill in the blanks. Now, tell me what happened to your foot,” said Tracy, kindly.
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“Well, I blame my cousin, Nicole” laughed Katrina. She went on to explain that in trying to help her lose weight and get healthy, her favorite cousin Nicole had gotten Katrina to walk on the trail in the local park three times a week. Her mom wanted to be supportive of the effort and bought Katrina a new pair of walking shoes. Unfortunately she rubbed a blister the first day and it had not healed. “Nikki’s efforts at getting me fit seem to have backfired. Now it is hard to walk at all!” explained Katrina.
Tracy asked Katrina more questions as she made notes in her chart. “Well. We have arrived at the portion of our program where Dr. Carter is required.” Tracy handed Katrina a couple of magazines. “I shall leave you for the moment to catch up with fashion and such. Dr. Carter will be here in a jiffy!” smiled Tracy as she gently closed the exam room door.
A few minutes later there was a knock at the door and a tall, brown haired man opened the door as Katrina said, “come in?”
“Hi, Katrina, I am Dr. Carter,” greeted the man. Tracy followed him in. “I thought I would stay since Katrina’s mom couldn’t be here.”
Katrina smiled at her gratefully as she sat the magazine aside. She was a bit nervous, she admitted to herself. “Katrina,” began Dr. Carter after swiftly performing some quick assessments by looking in her throat and nose, feeling her neck and listening to her heart and breathing with his stethoscope. “I know we have just met and this may seem very harsh but I need to be very direct with you. You are morbidly obese and in danger of a variety of diseases, all which could shorten your life.”
“That is certainly direct!” exclaimed Katrina. “I knew I hadn’t felt good in a while but I didn’t think I had anything that losing some weight and exercising wouldn’t fix. I thought my main problem was the sore on my foot and you haven’t even looked at that yet.”
“The sore, while painful, is just a symptom of a bigger problem.” He said as he gently peeled the bandage from her foot. “I see flip flops have been the choice of footwear.”
“All my other shoes rub the bandage off.” She replied. “Luckily, I have several pair!” What do you think is wrong with me?” she asked nervously.
“Well,” Dr. Carter responded, as he cleaned her sore with antiseptic, applied an antibiotic ointment and re-bandaged her foot, “I think you may have a problems stemming from your weight and lifestyle choices and genetics may play a part too. Your pulse and blood pressure are also higher than they should be. The urinalysis we did suggests your glucose levels are way too high. I am going to get some lab work done to find out for sure. We will make another appointment when the results come in and I will be able to make a more certain diagnosis. I will want your mom to come with you so we can talk about everything together.”
“I know what I said to you was shocking, but I want you to know how serious your situation is. The blister on your foot may well have saved your life because it got you in to see a doctor. One of the studies I am asking you to do is called a fasting glucose test. You will have to miss a day of school and won’t be allowed to eat anything for 8 hours before the test. They will
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draw a blood sample and then have you drink some glucose and after a bit they will take another blood sample. I will also have the lab analyze other aspects of your blood to determine how well your liver, kidneys, thyroid and other organs are functioning along with cholesterol and triglyceride levels.” Dr. Carter explained. “Do you know what those are?” he asked?
“I have an idea,” she replied. “We are starting to study macromolecules in biology this week so I know I will be learning more.”
“That is good,” he replied, “Knowledge is power!” While what I said about your health is very serious, the good news is your outcome can change for the better with the right treatment and life-style changes.” He paused for a moment to wash his hands.
“From the looks of your foot you were pretty dedicated to exercising with your cousin. We need to make sure you are healthy enough to continue your exercise program. I will give you a prescription for some oral antibiotics along with this ointment,” he said as he handed her the tube. “Keep the wound covered while you are at school so it will stay clean but if you are home, prop your foot and take the bandage off.” Katrina nodded her understanding as she took the tube of ointment.
You will definitely need to change your diet too. Tracy tells me you have lost some weight. What have you done to lose the five pounds?”
“Well, I stopped drinking sodas and starting drinking water instead. I thought I would start small and work towards changing my diet. I figured I would get discouraged if I couldn’t eat some of the things I enjoy. I was going to give up my afterschool candy next,” offered Katrina.
Dr. Carter smiled at her, “That’s a great start! I am going to suggest some other changes too and will be sending you to see a nutritionist who can help you plan a better diet. Once we determine what your health situation is we can get you feeling better and you will enjoy being more active.”
Tracy turned to Katrina after Dr. Carter left the exam room, “are you ok? That was pretty tough to hear.”
Katrina smiled back at her thoughtfully. “It is like I told my mom the other day. I know I am overweight and have health issues. Dr. Carter just told me straight up I will have to deal with them or have a short life. I am glad he was so direct and didn’t try to make me feel better about my weight. It would have been easier to keep kidding myself that it is OK to weigh almost 300 pounds at my age but it wouldn’t have helped me in the long run.”
Tracy gave her a quick hug after handing her the information sheet for the lab and a new appointment card. “You are a very brave kiddo” she said. “I’ll see you on Thursday!”
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A Taste of Trouble Part III: Lab Results and Normal Range Information
Fasting Blood GlucoseGLUCOSE LEVEL INDICATION
From 70 to 99 mg/dL (3.9 to 5.5mmol/L Normal fasting glucoseFrom 100 to 125 mg/dL (5.6 to 6.8 mmol/L Impaired fasting glucose (pre-diabetes)126 mg/dL (7.0 mmol/L) and above on more than one testing occasion Diabetes
Oral Glucose Tolerance Test (OGTT)Levels applicable except during pregnancy. Sample drawn 2 hours after a 75-gram glucose drink.Less than 140mg/dL (7.8 mmol/L) Normal glucose toleranceFrom 140 to 100 mg/dL (7.8 to 11.1 mmol/L) Impaired glucose tolerance (pre-diabetes)Over 200 mg/dL (11.1 mmol/L on more than one testing occasion Diabetes
American Association for Clinical Chemistry
Health Links Inc. 100 Somewhere Road Small Town, OK 12345CLIA 35X2187908Patient: Katrina Martin D.O.B. 06/08/1994 16Y/F Pt. # 987654321 Acct# A045320988215Physician: John Carter Acct # 45-455610Date: September 14, 2010Specimen Received Date and Time: September 14, 2010 10:00 AMChemistry
Name Result Flag Normal RangeGlucose 302c* (C) 70-110 mg/dLSodium 149 (H) 135 – 14 mEq/LPotassium 4.2 3.5-5.5 mEq/LChloride 95 (L) 96-113 mEq/LCO2 25 21-34 mEq/LBUN 11 6-23 mEq/LCreatinine 1.1 (H) 0.2 – 0.6 mEq/L
C* = comment C= Critical H= High L=Low D=Delta
c* Critical High Glucose value called to Dr. Carter’s office. Results given to nurse Tracy HowardJohn Q Tech, MT Sept 14, 2010 10:50 AM
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A Taste for Trouble Part IV
Katrina knocked on Sharon Smalley’s office door. “Come in!” shouted Sharon. Katrina poked her head around the door to see Sharon tittering on a stool as she reached up to the top shelf of her office bookcase.
“Well that certainly looks dignified!” exclaimed Katrina. “What if I was the president of the hospital or something!”
Sharon laughed as she caught hold of the book she had been trying to snag. “I wouldn’t care. Maybe they would get me a better bookcase or catch me if I fell!” she exclaimed. “You are looking healthier since the first time I met you. How are you feeling?”
“Oh tons better! My foot has healed and I am not as tired as I was. I sleep better too. I don’t have to get up all the time for a drink or to use the rest room. Mom says the food bill is way down too,” she giggled.
“That is good news! Tell me how checking your blood sugar is going? Are you finding a good routine and not skipping any blood checks?” Sharon inquired as she settled back into her desk chair.
“Yup! Although poking myself with needles several times a day is not my fav it sure beats the way I had been feeling.” She sat her bag down and reached for the book Sharon was handing her. “Gee whiz, this is a big book. Is lifting this several times a day my new exercise program?”
“No silly! This is a little light reading for your spare time.” She smiled as Katrina looked aghast! I am kidding, sort of. This is a really good reference book on biochemistry.” Katrina looked more aghast if possible. “I used it in my college nutrition class and I thought I would lend it to you for a while.”
“Gee, thanks!” Katrina responded slowly. Her mom had taught her to always be polite.
“Last time you were here you told me you were starting a unit about macromolecules at school. This book has a lot of reference material in it you might find useful. It is well written and explains things very clearly. I think you will find your diabetes to be more manageable the more you understand what is happening in your body.” Sharon smiled at Katrina.
“I don’t usually go to these depths with a client but I feel you will benefit from understanding the information on a deeper level. Your biology teacher is a friend of mine and I know she has some labs planned and you have an excellent biology text. Understanding the biochemistry helped me manage my own health when I was first diagnosed back in college. It also helped me to decide what I wanted to do as a career,” she explained as she leaned back in her chair.
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“I’m not sure I want to be a nurse,” said Katrina doubtfully as she looked down at the book.
Sharon laughed, “I’m not trying to convert you to a new career path, just help you educate yourself. The book is just a loner. I want it back when you are done.” She winked a Katrina. “But take your time I am not eager to climb back up to put it away.”
“Now,” she said leaning forward and brushing her hair out of her eyes with the back of her hand, “let’s talk turkey! Portion size that is!” she reached for a deck of cards lying on her desk.
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
TRAINING LAB – IDENTIFYING ORGANIC MOLECULES IN FOODS
BACKGROUND: You now know how important lipids, proteins, and carbohydrates are in your diet and how your body uses these molecules once you eat them. Is there a way to tell if these nutrients are actually present in the foods we eat? YES!! There are several commonly used tests for organic molecules.
SAFETY PRECAUTIONS!!!! WEAR YOUR GOGGLES AT ALL TIMES!! Be careful with solutions – wash your hands thoroughly after we are finished. Lugol’s Iodine Solution will stain your clothing. Report any broken glass immediately!!!!
PROCEDURES:
10. Completing the following tests:-Biuret Test-Lugol’s Iodine Test-Benedict’s Test
11. Complete the above tests on the following food molecules:-Distilled water -Sucrose solution
-Dilute glucose solution -Starch solution
-Concentrated glucose solution -Protein solution
12. EACH PERSON SHOULD RECORD THE DATA COLLECTED FROM THIS TRAINING LAB IN THEIR OWN TABLE.
13. Get six test tubes in a beaker. Label your tubes with the 6 food molecule solutions to be tested using clear tape and a ball point pen, pencil or Sharpie® pen.
14. THE BIURET TESTa. Add 20 drops of each food molecule solution to their respective test tubesb. Add 3 drops of Biuret Test solution to each test tube.c. Observe and record the results in your table (look for a color change)d. Rinse out your test tubes
15. THE LUGOL’S IODINE TESTa. Add 20 drops of each food solution to their respective test tubesb. Add 1 or 2 drops of Lugol’s Iodine Test Solution to each tubec. Observe and record the results in your table (look for a color change)d. Rinse out your test tubes.
16. THE BENEDICT’S TESTa. Add 20 drops of each food molecule solution to their respective test tubesb. Add 10 drops of Benedict’s Test Solution to each tube.c. Place tubes in a hot water bath for 5 minutesd. Immediately observe the results and record your observations in your data table.e. CLEAN UP!!!! Remove any labels from your test tubes, rinse them out and
then wash them in the soapy water using the test tube brush. Rinse again and turn upside down in the container to dry.
G & C Ulmer
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
My lab partner’s name is _________________________________.
Result Table:
Distilled
H2O
Dilute
Glucose
Solution
Conc.
Glucose
Solution
Sucrose
Solution
Starch
Solution
Protein
Solution
Biuret
Benedict’s
Lugol’s
Notes:
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
QUESTIONS: Identifying Organic Compounds in Foods
8. Carefully fill in the table with observational data. This table will be used to determine what molecules are present in an unknown solution.
9. What solution was included in this lab as a CONTROL to help you interpret your results? _________________________
10. Which test would you use if you wanted to detect the presence of a protein in an unknown solution? __________________________How could you determine if a protein was present?
11. Which test would you use if you wanted to detect the presence of a starch in an unknown solution? ______________________. How would you know if starch was present?
12. Which test would you use if you wanted to detect the presence of glucose in an unknown solution? _____________________ How would you tell if glucose was present?
13. You are asked to analyze a food substance to determine what organic food molecules are present. You observe a positive reaction with Benedict’s and Biuret solutions. What can you conclude about this food? (A positive reaction means that there was a color change.)
14. Do you have any way of telling how concentrated the glucose is in a food sample? _____ if yes – explain how, if you answered no, draw a picture of your favorite food.
17. Do you have any way of determining if sucrose is present in a food sample? ________ Explain your answer.
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A Taste of Trouble: McMush Lab
Just what is in a fast food meal??? We hear that fast food meals are "bad", but do they have anything good in them? Can they provide some nutrition? Are they really high in fat?
Well, let's find out!!!
Carbohydrates, proteins, fats, vitamins, and other nutrients provide your body with energy and provide the raw materials necessary to carry on life activities. These compounds are present in the plants and animals you use as food. In this lab, you will test for specific compounds and then determine if these are in a fast food value meal in sufficient quantities.
Materials:
McDonald's value mealBenedict's solution- tests for sugar (carbohydrates)Biuret solution- tests for proteinLugol's iodine solution- tests for starch (complex carbohydrates)Silver NitrateBrown paper (craft paper or a brown paper bag)Blender- it must be mixed together to test it!!
SAFETY NOTE!!! FOR THIS LAB, YOU MUST WEAR EYE PROTECTION!!!
To determine if these substances are in a sample of McMush, it is important to know what a positive test looks like for each of these components. So, the first part of the experiment will be to run tests on known samples to discover what a positive test looks like. If you have already done the Training Lab for Macromolecule ID just do the lipid and sodium test before going on to Part II.
Part I: Testing of Known Substances
Protein test: 1. Place 5 ml of the protein solution (this is actually ground up egg white, which is pure protein)
into a test tube.2. Add ten drops of Biuret solution.3 Observe any color change. Record it in chart.
Glucose test (simple sugar):
1. Place 5 ml of the glucose solution into your test tube.2. Add 3 ml of Benedict's solution. Place the tube in a beaker of boiling water and boil for five
minutes. Use test tube clamps to hold hot test tubes.3. Observe any color change. Record it in chart.
Starch test (complex carbohydrate):
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
1. Place 5 ml of the starch solution into a test tube. 2. Add 5 drops of Lugol's iodine solution. 3. Observe any color change. Record it in chart.
Lipid Test
4. Place 1 ml of vegetable oil on one small area of a paper bag. On another area, place a small amount of butter.
5. After a few seconds, remove the oil and butter with a paper towel. 6. Foods that contain lipids will leave a translucent mark on brown paper bag material. Record
results in the chart.
Sodium Test
5. Place 2 ml of salt solution in a test tube.6. Add 1 ml of distilled water (for a 3 ml total of solution)7. Transfer 2-3 drops of silver nitrate solution to a test tube.8. A cloudy solution indicates the presence of sodium; a precipitate may form.
Record your results in a data table:
Food Substance Reagent test Positive Results
Protein Biuret solution
Sugar Benedict's solution
Starch Lugol's iodine solution
Lipid Brown Bag
Sodium Silver nitrate
NOTES and Observations:
Modified from J. Brown
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Bio 529 Case Study A Taste for Trouble Linda Kay Gamble
Part II: McMush!
The McDonalds Value Meal will be blended and for a sample of "filtered McMush" to work with.
1. Repeat the reagent tests above using 5 ml of the McMush solution for each test (except for the lipid test, where only 1 ml of filtered McMush is needed, and the sodium test, using 2 ml of filtered McMush solution).
2. Describe and record results in the following table.
Food Substance Reagent test Test Results
Protein Biuret solution
Sugar Benedict's solution
Starch Lugol's iodine solution
Lipid Brown Bag
Sodium Silver nitrate
Total Fat Boil/Cool
PART III: McMush - Demonstration of Fat Content. This could be done as a demonstration.
Materials:
McMush 1 hot plate 1 - 500 ml beaker 1 - 200 ml graduated cylinder
100 ml of water 2 oven mitts 1 wooden spoon 1 refrigerator to cool McMush mixture
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Procedure Overview:
5. The complete meal will be blended to make the McMush.6. A large sample of the meal will be heated.7. From the heated sample, 100 ml will be taken to be cooled.8. Results from the 100 ml sample are representational of the complete meal.
Procedure: 11. Preheat the hot plate.12. Break up meal into small pieces and blend.13. Pour part of the blended McMush into a 500 ml beaker.14. Add 100 ml of water to the McMush and stir well.15. Boil McMush mixture gently for 15 minutes. 16. Use oven mitts to protect your hands and pour the warm McMush mixture into a graduated
cylinder. Then cool in the refrigerator for 15 minutes. This can stay in the refrigerator overnight if necessary but cover it with aluminum foil or plastic wrap.
17. Remove McMush from the refrigerator and measure the amount of accumulated fat at the top of the graduated cylinder.
18. Record results.19. Dispose of mixture according to the teacher’s directions.20. Clean glassware with warm soapy water.
Expected Outcome:
The fat will form a layer at the top and solidify as it cools. Calculate the percent of fat in the McMush meal by dividing the ml of fat by the total mL of the sample. For example, if there is 40 mL of fat out of a total of 100 ml of sample. This would indicate the total meal contained 40% fat.
Conclusion:
Write the results to this lab as a letter to Katrina telling her the results of the test and what your recommendations would be about eating A Happy Meal at McDonalds.
How do you think a Happy Meal compares to other fast food meals? How would you go about testing your hypothesis? Describe how you would design an experiment to compare other foods to the McMush experiment.
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A Taste for Trouble Part V: Katrina was actually the first one in the room that morning. Her science teacher looked up from
the paper she was reading and smiled. “Good morning Katrina! I was just reading the essay you wrote
about the video “SuperSize Me!” You really have some strong feelings about fast food!”
Katrina grinned, “Well, it has been a love/hate relationship. I am happy to say I have kicked the
habit. It was very emotional watching the video knowing I had done so much harm to my body by eating
too much fast food. I never realized I was in the grip of an addiction. Knowledge is power, as my doctor
is fond of saying!”
“It sure is! My job is all about giving you guys lots of power! She smiled as she went to the back
of the classroom.
Jeri drooped into a chair. Her eyes lit up when she saw Katrina already in the room. “Ah! BFF
you wouldn’t have a spare candy bar on you would you? I didn’t get a chance to eat breakfast this
morning and I am feeling really shaky.”
Katrina reached into her enormous bag and pulled out a banana, “Your in luck, Jeri, I have
something even better!”
Jeri sighed as she gratefully took the banana. “Yeah, your right but I do miss the big bag full of
candy sometimes.”
“I still think you need to go see Dr. Carter. He told me sometimes people have low blood sugar
and it can lead to diabetes too. Not a fun disease to have. I am determined to lose enough weight so
maybe I can stop taking insulin.
“You’re probably right,” sighed Jeri. “I should learn by your example. I’ll talk to my mom about
getting an appointment. Hey, I wonder if there are other diseases caused by nutrition. Maybe that is
what we can do for our biology research project.” She moved her book bag out of the way as students
began pouring into the classroom.
“I’ll bet there are. It seems you really are what you eat. I was watching Discovery channel last
night and they said more people are suffering from diabetes in other countries too. It is becoming an
epidemic everywhere. I was thinking our research project could be about how nutrition is changing in
developing countries and causing an increase in diabetes in countries where it wasn’t a problem before.
How sad! My old beloved fast food may be causing disease across the world!”
“That sounds like a great idea, Katrina,” their science teacher remarked as she returned to the
front of the room. “Let’s get started!”
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Bio 520 Case Study A Taste for Trouble Linda Kay Gamble
A Taste for Trouble Culminating Project
You must:
1. Research the effects of diabetes on the body.
2. Find and give a brief summary of 2 other diseases whose predominant cause is diet and lifestyle choices.
3. Find information about global increases in these diseases over the past 20 years.
4. Turn in your bibliography and references when you present your project.
Choose a format to present your findings:
7. Video news report
8. Pamphlet
9. Radio Spot or podcast
10. Poster
11. Story book
12. Other (make sure you get teacher approval)
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