Lessons from sea louse and salmon epidemiologymlewis/Publications 2016/Groner et...Figure 1. Data and models are key components in the scientific process. Although data and models

Seediscussions,stats,andauthorprofilesforthispublicationat:https://www.researchgate.net/publication/294728721

Lessonsfromsealouseandsalmonepidemiology

ArticleinPhilosophicalTransactionsofTheRoyalSocietyBBiologicalSciences·March2016

DOI:10.1098/rstb.2015.0203

CITATIONS

4

READS

179

12authors,including:

Someoftheauthorsofthispublicationarealsoworkingontheserelatedprojects:

Assessmentofchangesinstock-recruitmentrelationshipsViewproject

DispersaloftheinvasivealgaCodiumfragileinseagrassmeadowsViewproject

NeilFrazer

UniversityofHawaiʻiatMānoa75PUBLICATIONS1,433CITATIONS

SEEPROFILE

StephanieJanePeacock

TheUniversityofCalgary

11PUBLICATIONS98CITATIONS

SEEPROFILE

ErinERees

LandandSeaSystemsAnalysisInc.

30PUBLICATIONS343CITATIONS

SEEPROFILE

CrawfordWRevie

UniversityofPrinceEdwardIsland

137PUBLICATIONS2,260CITATIONS

SEEPROFILE

AllcontentfollowingthispagewasuploadedbyBrendanMConnorson22February2016.

Theuserhasrequestedenhancementofthedownloadedfile.Allin-textreferencesunderlinedinblueareaddedtotheoriginaldocument

andarelinkedtopublicationsonResearchGate,lettingyouaccessandreadthemimmediately.

https://www.researchgate.net/publication/294728721_Lessons_from_sea_louse_and_salmon_epidemiology?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_2&_esc=publicationCoverPdfhttps://www.researchgate.net/publication/294728721_Lessons_from_sea_louse_and_salmon_epidemiology?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_3&_esc=publicationCoverPdfhttps://www.researchgate.net/project/Assessment-of-changes-in-stock-recruitment-relationships?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_9&_esc=publicationCoverPdfhttps://www.researchgate.net/project/Dispersal-of-the-invasive-alga-Codium-fragile-in-seagrass-meadows?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_9&_esc=publicationCoverPdfhttps://www.researchgate.net/?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_1&_esc=publicationCoverPdfhttps://www.researchgate.net/profile/Neil_Frazer2?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_4&_esc=publicationCoverPdfhttps://www.researchgate.net/profile/Neil_Frazer2?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_5&_esc=publicationCoverPdfhttps://www.researchgate.net/institution/University_of_Hawaii_at_Mnoa?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_6&_esc=publicationCoverPdfhttps://www.researchgate.net/profile/Neil_Frazer2?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_7&_esc=publicationCoverPdfhttps://www.researchgate.net/profile/Stephanie_Peacock2?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_4&_esc=publicationCoverPdfhttps://www.researchgate.net/profile/Stephanie_Peacock2?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_5&_esc=publicationCoverPdfhttps://www.researchgate.net/institution/The_University_of_Calgary?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_6&_esc=publicationCoverPdfhttps://www.researchgate.net/profile/Stephanie_Peacock2?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_7&_esc=publicationCoverPdfhttps://www.researchgate.net/profile/Erin_Rees?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_4&_esc=publicationCoverPdfhttps://www.researchgate.net/profile/Erin_Rees?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_5&_esc=publicationCoverPdfhttps://www.researchgate.net/profile/Erin_Rees?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_7&_esc=publicationCoverPdfhttps://www.researchgate.net/profile/Crawford_Revie?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_4&_esc=publicationCoverPdfhttps://www.researchgate.net/profile/Crawford_Revie?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_5&_esc=publicationCoverPdfhttps://www.researchgate.net/institution/University_of_Prince_Edward_Island?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_6&_esc=publicationCoverPdfhttps://www.researchgate.net/profile/Crawford_Revie?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_7&_esc=publicationCoverPdfhttps://www.researchgate.net/profile/Brendan_Connors?enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg%3D%3D&el=1_x_10&_esc=publicationCoverPdf

on February 15, 2016http://rstb.royalsocietypublishing.org/Downloaded from

rstb.royalsocietypublishing.org

ReviewCite this article: Groner ML et al. 2016Lessons from sea louse and salmon

epidemiology. Phil. Trans. R. Soc. B 371:20150203.

http://dx.doi.org/10.1098/rstb.2015.0203

Accepted: 18 December 2015

One contribution of 14 to a theme issue

‘Marine disease’.

Subject Areas:ecology, health and disease and epidemiology,

theoretical biology

Keywords:Atlantic salmon, ecological modelling,

emerging infectious disease, fish farm,

marine disease, Pacific salmon

Author for correspondence:Maya L. Groner

e-mail: [email protected]

†These authors contributed equally to this

work.

& 2016 The Author(s) Published by the Royal Society. All rights reserved.

Lessons from sea louse and salmonepidemiology

Maya L. Groner1,†, Luke A. Rogers2,†, Andrew W. Bateman2,3,4, BrendanM. Connors4,5,6, L. Neil Frazer4,8, Sean C. Godwin7, Martin Krkošek2,4,Mark A. Lewis3,9, Stephanie J. Peacock3, Erin E. Rees1, Crawford W. Revie1

and Ulrike E. Schlägel9

1Department of Health Management, Centre for Veterinary and Epidemiological Research, Atlantic VeterinaryCollege, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island,Canada C1A 4P32Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario,Canada M5S 3B23Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E94Salmon Coast Field Station, Simoom Sound, British Columbia, Canada V0P 1S05ESSA Technologies Ltd, Vancouver, British Columbia, Canada V6H 3H46School of Resource and Environmental Management, and 7Earth to Ocean Research Group, Department ofBiological Sciences, Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S68Department of Geology and Geophysics, School of Ocean and Earth Science and Technology, University ofHawaii at Manoa, Honolulu, Hawaii 96822, USA9Department of Mathematical and Statistical Sciences, University of Alberta, Edmonton, Alberta,Canada T6G 2G1

MLG, 0000-0002-3381-6415

Effective disease management can benefit from mathematical models thatidentify drivers of epidemiological change and guide decision-making. Thisis well illustrated in the host–parasite system of sea lice and salmon, whichhas been modelled extensively due to the economic costs associated withsea louse infections on salmon farms and the conservation concerns associatedwith sea louse infections on wild salmon. Consequently, a rich modelling lit-erature devoted to sea louse and salmon epidemiology has been developed.We provide a synthesis of the mathematical and statistical models that havebeen used to study the epidemiology of sea lice and salmon. These studiesspan both conceptual and tactical models to quantify the effects of infectionson host populations and communities, describe and predict patterns of trans-mission and dispersal, and guide evidence-based management of wild andfarmed salmon. As aquaculture production continues to increase, advancesmade in modelling sea louse and salmon epidemiology should inform thesustainable management of marine resources.

1. IntroductionReductions in marine fish abundance due to overfishing are predicted to reducepathogen diversity by ‘fishing-out parasites’ [1,2]. At the same time, the expan-sion of industrial aquaculture over the past 50 years has created new pathwaysand reservoirs for pathogens [3,4]. Farmed fish are often exposed to pathogensfrom sympatric wild fish populations, but are sheltered from many of the natu-ral processes (e.g. predation [5], competition [6], migration [7,8]) that canremove infected individuals from populations. Farmed host populations aretypically held at higher densities than populations in the wild, increasingopportunities for infections to spread. Understanding the effects of anthropo-genic changes on marine disease in wild and farmed host populations istherefore a conservation and management priority.

Mathematical models can inform marine conservation and management byaddressing questions such as: When can a pathogen invade a population? Whatimpact will it have on the population? Will it cause a chronic or acute infection?

http://crossmark.crossref.org/dialog/?doi=10.1098/rstb.2015.0203&domain=pdf&date_stamp=2016-02-15mailto:[email protected]://orcid.org/http://orcid.org/0000-0002-3381-6415http://rstb.royalsocietypublishing.org/https://www.researchgate.net/publication/49771944_Animal_Migration_and_Infectious_Disease_Risk?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/43297938_Fishing_out_marine_parasites_Impacts_of_fishing_on_rates_of_parasitism_in_the_ocean?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/294596236_Fishing_diseased_abalone_to_promote_yield_and_conservation?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/43446640_The_Rising_Tide_of_Ocean_Diseases_Unsolved_Problems_and_Research_Priorities?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/284001207_Theoretical_insight_into_three_disease-related_benefits_of_migration?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/277595370_Sea_lice_sockeye_salmon_and_foraging_competition_Lousy_fish_are_lousy_competitors?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/233332595_Impact_of_parasites_on_salmon_recruitment_in_the_Northeast_Atlantic_Ocean_Proc?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==

data

statistical inferenceinfer unknowns byconnecting models to data — parameter estimation — model selection

model simulationevaluate realisticscenarios using thecomputer andestimated parameters

past and present future

understanding managing

fieldwork and experiments — observe phenomena — visualize data — descriptive statistics

motivating

estimate relationshipsbetween variables [21]test hypotheses [3,59]explain underlyingmechanisms [37]

project behaviours ofsystems [20,28,43]predict outcomes ofinterventions [15,27]

raise questions andgenerate hypotheses[66]propose models [49]

present

defi

nitio

nfu

nctio

ns a

nd g

oals

times

cale

find possibleexplanations for patternsand phenomena [18]generate hypotheses[4]specify new data needs[27]

exploring

assumed and potential

model analysistheoretically investigatemechanisms of a system

models

Figure 1. Data and models are key components in the scientific process. Although data and models can be considered independently, the greatest advances inunderstanding the ecology and epidemiology of salmon and sea lice have been made by confronting models with data through statistical inference and modelsimulation. (Online version in colour.)

rstb.royalsocietypublishing.orgPhil.Trans.R.Soc.B

371:20150203

2


and How do ecological, anthropogenic and environmental dri-vers influence these patterns? While many questions in marineepidemiology remain unsolved, a growing body of quantitat-ive research has tackled the interface of anthropogenicchange, pathogen transmission and effects of infection onhost populations. These models can test hypotheses, explainpatterns or project management outcomes (figure 1) and canspan the individual, population, community or ecosystem.

Progress in the field of marine epidemiology is well demon-strated by studies of sea louse infections. Sea lice (Lepeophtheirussalmonis and Caligus spp.) infecting salmon (Oncorhynchus spp.and Salmo spp.) have gained considerable attention (reviewedhere and in [9–11]) due to economic costs associated with sealouse infections on salmon farms [12] and conservation con-cerns associated with sea louse infections on wild salmon[13]. Many models have been developed to study this system,ranging from statistical to dynamic and theoretical to applied.These models borrow insight from ecology, oceanography,epidemiology and physics (table 1). The complexity ofsalmon and sea louse epidemiology is such that no single math-ematical model can capture it. Model types range fromnonlinear dynamical systems, to regression, to individual-based simulation, to numerical ocean-circulation calculations,reflecting the broad spectrum of approaches to investigation.In short, sea lice and salmon make a convenient system forcataloguing the strategies used to model marine diseases.

Here, we showcase quantitative methods and advancesfrom the epidemiology of sea lice on wild and farmedsalmon hosts (figure 2). We focus on interactions betweenhost salmon, parasitic sea lice and the environment(figure 3) and their relevance to transmission and outbreakdynamics, conservation and the sustainable managementof wild fisheries and farmed salmon. As concerns aboutpathogen-mediated interactions between farmed and wildmarine species rise, the lessons learned from sea louse andsalmon epidemiology are likely to be increasingly relevant.

2. Outbreak and transmission dynamicsUnderstanding when and why outbreaks occur and themeans by which they spread is essential for developing amechanistic understanding of marine diseases. Transmissiondynamics in the ocean are variable and complex due to fluc-tuating water chemistry, oceanographic mixing and, in manycases, free-living infectious stages. This can be further compli-cated by how aquaculture moves, manages and manipulateshost populations.

Most theoretical models assume that increased hostpopulation density favours parasite transmission [30].Indeed, with the exception of vector-borne and sexuallytransmitted diseases, parasite populations are expected togrow only when hosts exceed a threshold density [31,32].The presence of host-density dependence in sea louse trans-mission suggests that an increase in host density can triggera transition from chronic to acute sea louse outbreakdynamics [33,34]. Statistical models of sea louse populationdynamics in Norway [35], stochastic network modelsof sea louse infection in Loch Fyne, Scotland [36], andhydrodynamic simulations of sea louse production and con-centrations in the Broughton Archipelago, British Columbia[28], show how sea louse dynamics can respond to changesin host density among multiple salmon farms and wildsalmon populations. Consequently, the relevant spatialscale for critical host-density thresholds in sea lousedynamics, might encompasses several salmon farms, therebyconstraining regional production [34].

Temporal variation in host population density can alterepidemiological patterns. Regional host densities in coastalseas experience seasonal pulses of wild salmon biomass.The 1.5- to 2-year seawater production cycles for farmedsalmon add additional variability in host density in coastalregions. During spring out-migrations, uninfected juvenilesalmon leave their natal streams and estuaries, and migrate

http://rstb.royalsocietypublishing.org/https://www.researchgate.net/publication/12738603_Sealice_on_Salmonids_Their_Biology_and_Control?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/24042912_The_global_economic_cost_of_sea_lice_to_the_salmonid_farming_industry?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/6868215_Ecology_of_sea_lice_parasitic_on_farmed_and_wild_fish?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/232809965_Use_of_agent-based_modelling_to_predict_benefits_of_cleaner_fish_in_controlling_sea_lice_Lepeophtheirus_salmonis_infestations_on_farmed_Atlantic_salmon_Salmo_salar_L?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/25453941_Host_Densities_as_Determinants_of_Abundance_in_Parasite_Communities?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/221818005_Sea_lice_as_a_density-dependent_constraint_to_salmonid_farming?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/221818005_Sea_lice_as_a_density-dependent_constraint_to_salmonid_farming?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/260127207_Modelling_the_Impact_of_Temperature-Induced_Life_History_Plasticity_and_Mate_Limitation_on_the_Epidemic_Potential_of_a_Marine_Ectoparasite?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/273920320_Host_density_thresholds_and_disease_control_for_fisheries_and_aquaculture?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/229414188_Assessing_topical_treatment_interventions_on_Scottish_salmon_farms_using_a_sea_lice_Lepeophtheirus_salmonis_population_model?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/250924474_Sea_lice_infestations_on_juvenile_chum_and_pink_salmon_in_the_Broughton_Archipelago_Canada_from_2003_to_2012?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/279195813_Modelling_Infectious_Hematopoietic_Necrosis_Virus_Dispersion_from_Marine_Salmon_Farms_in_the_Discovery_Islands_British_Columbia_Canada?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/233790873_Connectivity_modelling_and_network_analysis_of_sea_lice_infection_in_Loch_Fyne_west_coast_of_Scotland?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/236887686_Space-Time_Modelling_of_the_Spread_of_Salmon_Lice_between_and_within_Norwegian_Marine_Salmon_Farms?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/236193716_Modeling_Parasite_Dynamics_on_Farmed_Salmon_for_Precautionary_Conservation_Management_of_Wild_Salmon?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/247205739_Population_Biology_of_Infectious_Diseases?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==

Table 1. Types of models and their application to the salmon sea louse pathosystem. Key references are included.

model form characteristics applications and examples

difference equations —discrete-time dynamics

—spatially homogeneous

—deterministic

—can include delays

population growth models, e.g. Ricker stock –

recruitment relationship [14]; often used within other

models

—effects of lea louse infection and predation on salmon

productivity [15]

ordinary differential

equations

—continuous-time dynamics

—spatially homogeneous

—deterministic

—can include delays

host – parasite models for louse-salmon dynamics, e.g.

Anderson – May model [16] and extensions thereof to

incorporate predation

—effects of treatments on sea louse populations on

farms [17]

—parasite-mediated changes to predation [18]

partial differential

equations

—continuous-time dynamics

—spatially heterogeneous

—deterministic

advection-diffusion models

—sea louse dispersal from salmon farms along wild

salmon migration routes [19]

matrix models (Leslie

matrix, population

projection matrix)

—linear system of difference equations

—can include stochastic effects

age-structured population growth models

—analysis of temperature-dependent sea louse

demography [20]

regression models —statistical

—descriptive/correlational

—can include spatial effects

GLM, GLMM, random effects, logistic regression

—identifying epidemiological factors effecting sea lice

abundance on salmon farms [21]

—associations between aquaculture and sea louse

infections on sea trout [22]

survival functions and

hazard functions

—statistical

—descriptive/correlational

survival analysis

—impacts of sea lice on salmon survival in the NE

Atlantic [3]

—effects of salinity on sea louse survival on juvenile

salmon [23]

stochastic processes —discrete-time or continuous-time dynamics

—stochastic

—can include additional hierarchy

stochastic population growth models, e.g. stochastic

Ricker model

—hierarchical models of Pacific salmon productivity in

relation to sea lice [24 – 26]

individual-based (or agent-

based) model

—computer model

—simulates actions and interactions of individuals within

a system

—can be deterministic or stochastic

predicting benefits of cleaner fish in control of sea

louse populations [27]

numerical ocean-circulation

model

—numerically solved complex dynamical system; includes

hydrodynamic equations, three-dimensional transport

and diffusion equations)

—realistic model for oceanic motion

model for current, temperature and salinity patterns in

marine environment

—finite volume coastal ocean model (FVCOM) simulation

of the spread of sea lice from salmon farms in British

Columbia, Canada [28]

—SINMOD simulation of sea louse and salmonid

pancreatic disease virus in Norwegian fjords [29]


371:20150203

3


through coastal waters to the open ocean. Parametrizedadvection-diffusion-decay models (table 1) of sea louse dis-persal from salmon farms along juvenile salmon migrationcorridors demonstrate that infection pressure from farm-source sea lice can exceed background (e.g. wild-source)

infection pressure in the Broughton Archipelago, BritishColumbia, Canada [37]. The process of spill-over (fromwild to farmed hosts) and spill-back (from farmed to wildhosts) [38] of sea lice is likely initiated by adult wildsalmon that carry sea lice into coastal waters from the open

http://rstb.royalsocietypublishing.org/https://www.researchgate.net/publication/235773258_Transmission_dynamics_of_pancreas_disease_PD_in_a_Norwegian_fjord_Aspects_of_water_transport_contact_networks_and_infection_pressure_among_salmon_farms?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==

wild salmon distribution

mean annual farmed Atlanticsalmon production, 2003–2012 (tonnes)

10 000

100 000

1 000 000

Figure 2. Global distribution of wild and farmed salmon. Farmed salmon production data were obtained from the Food and Agriculture Organization of the UnitedNations (FAO) FishStat dataset and include only marine-farmed Atlantic salmon. Countries producing less than 5000 tonnes annually were excluded from the dataset.Circle areas are scaled by mean annual production. Canada is split into two farming regions to differentiate between its Pacific and Atlantic production. For somecountries, circles do not encompass all salmon farming locations; in these cases, circles cover the country’s region of highest farm density. Wild salmon distributiondata were digitized from the FAO’s Aquatic Species Distribution Map Viewer, which provides current distributions for Atlantic salmon and the five traditional Pacificsalmon species, and modified to exclude waters more than 50 km offshore. (Online version in colour.)


371:20150203

4


ocean during summer and autumn. The natural process ofmigratory allopatry, where uninfected juvenile salmon areseparated from adult salmon and protected from sea lice bymigration [39], can be interrupted by the presence of sealouse reservoirs on salmon farms. When these reservoirswere removed by fallowing or parasiticide treatment offarmed salmon, epizootics of sea lice on wild juvenilesalmon declined [40], helping to verify model predictions.

When the density of potential hosts exhibits spatial struc-ture (e.g. from farm placement within oceanographicregions) or spatial movement (e.g. from migrating wildsalmon populations), spatially explicit models can improvecharacterization of parasite transmission. Numerical simu-lations of ocean currents and wind in the BroughtonArchipelago, British Columbia, suggest that hydrodynamiccirculation can influence the connectivity between sea lousepopulations, potentially leading to between-farm source-sink dynamics [28]. By representing sea louse movement asparticle flow, these models can account for the influence ofwater circulation patterns and abiotic properties (e.g. temp-erature and salinity) on sea lice dispersal. Understandinginfective sea lice density and dispersal in the water columnis important for identifying farms or regions that are infectivesea lice sources and sinks (figure 4). Statistical models havefound spatio-temporal correlations between sea louse abun-dances on farmed and wild salmon up to 30 km apart[41,42]. When coupled with biological models, hydro-dynamic simulation models can be used to understand themechanisms driving these patterns and have been used tocharacterize transmission of salmon pancreas diseasevirus among salmon farms in Norway [29], and infectioushematopoietic necrosis virus between salmon farms and

wild salmon in Pacific Canada [43]. The contribution of indi-vidual farms within a network of farms to regional sea louseconnectivity has been quantified using graph theory [36,44]and by merging hydrodynamic modelling of dispersal withclassic host-macroparasite models to calculate local andregional thresholds for transmission (table 1) [16].

3. Implications for conservation and fisheriesIt is important to estimate pathogen impacts before takingmanagement actions. Models can provide the quantitativeframework with which to evaluate the evidence for the indi-vidual and combined impacts of different stressors (fishing,climate, competition, disease, etc.) on host population sizeand health. In the case of sea lice, mortality due to diseasemight be detectable in wild juvenile salmon, which arevulnerable to infections and many other mortality factors,as they migrate to sea [37]. However, if sea louse-inducedmortality is compensatory (those that die from infectionwere likely to have died for other reasons), it will not causea reduction in adults returning to spawn. Alternatively, ifmortality attributable to sea lice is additive to mortalityfrom other causes, such as predation, then overall mortalitywill increase and could lead to population decline. Thiswas exemplified in a predator–prey–parasite model ofinfected juvenile pink salmon [5]. The model incorporated ahypothesized saturating predation rate [45] of juvenile pinksalmon by juvenile coho salmon and an empirically derivedpositive relationship between predation on juvenilepink salmon and the infection level of the pink salmon [5].Analysis of the model suggested that when pink salmon

http://rstb.royalsocietypublishing.org/https://www.researchgate.net/publication/230693640_Regulation_and_Stability_of_Host-Parasite_Population_Interactions_I_Regulatory_Processes?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/235773258_Transmission_dynamics_of_pancreas_disease_PD_in_a_Norwegian_fjord_Aspects_of_water_transport_contact_networks_and_infection_pressure_among_salmon_farms?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/5905513_Effects_of_host_migration_diversity_and_aquaculture_on_sea_lice_threats_to_Pacific_salmon_populations?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/230693316_Some_Characteristics_of_Simple_Types_of_Predation_and_Parasitism?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/43520953_Modeling_population_connectivity_by_ocean_currents_a_graph-theoretic_approach_for_marine_conservation?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/279195813_Modelling_Infectious_Hematopoietic_Necrosis_Virus_Dispersion_from_Marine_Salmon_Farms_in_the_Discovery_Islands_British_Columbia_Canada?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/233790873_Connectivity_modelling_and_network_analysis_of_sea_lice_infection_in_Loch_Fyne_west_coast_of_Scotland?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/236580147_Understanding_sources_of_sea_lice_for_salmon_farms_in_Chile?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/237054764_Cessation_of_a_salmon_decline_with_control_of_parasites?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/274382917_Spatial_patterns_of_sea_lice_infection_among_wild_and_captive_salmon_in_western_Canada?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==

community effects competition, predation

evolution avoidance,

resistance, tolerance

reservoirsinteractions

betweenfarmed and wild

life cycle migration,

demography

abiotichydrodynamics,

temperature, salinity,

pollution, climate change

bioticfishing,

non-host species,parasite management

life cycle

density effects

mating strategies, dispersal,

transmission mode,generalist/specialistevolutionresistance to treatment,virulence

host[13,19]

environment[28]

pathogen[20]

disease[3,61]

[15,20]

[18,60,62,66]

[1,47]

Figure 3. The development of disease depends on factors of the host, parasite and environment. Studies often focus on one of these components, or the interactionbetween two, with relatively few studies considering all three components (illustrative references are provided in brackets). (Online version in colour.)


371:20150203

5


populations were small, infection increased juvenile pinksalmon mortality, whereas when they were large, infectionand predation were compensatory sources of mortality.

Several studies have evaluated evidence for population-level impacts of sea louse infection on wild salmon. Moststudies have been based on models of salmon spawner–recruitment dynamics, which investigate whether sea louseexposure can explain variability in the spawner–recruitmentrelationship (e.g. [42]) (figure 5a). In spawner–recruitmentmodels, a population’s growth rate during sea louse epidemicscan be compared to the population growth rate of the samepopulation before or after the epidemic, or to nearby, unex-posed salmon populations (figure 5b). Studies on wild pink(Oncorhynchus gorbuscha) [13,24,40] and coho (Oncorhynchuskisutch) [25] salmon in Pacific Canada found evidence thatpopulation growth rates are depressed in populations exposedto sea lice outbreaks associated with salmon aquaculture. Simi-larly, studies on post-smolt wild sea trout (Salmo trutta) inIreland found that infection levels were higher if the fishwere captured in bays that also contained salmon farms [46].Incorporating information on variability in infection pressurecan increase statistical power of such tests (figure 5c). Forexample, productivity of wild pink and coho salmon hasbeen found to negatively correlate with time-varying regionalsea louse abundance on farmed or wild salmon [26,40].

Considering multiple populations simultaneouslyincreases the chance that common responses to disease canbe separated from other sources of population variability[47]. This is most powerful when salmon populations areused as statistical replicates to identify the underlying‘signal’ of sea louse exposure, as opposed to single- or aggre-gate-population level evaluations. For example, analysesseeking to estimate sea louse-associated mortality in pinksalmon in Pacific Canada, based on a single aggregatestock–recruitment relationship [48], failed to detect a sealouse signal. However, a signal of sea louse exposure wasdetected using a multi-population analysis because it had

greater statistical power [26]. Observations from individualsalmon populations might not be independent if salmonpopulations are related to a common regional estimate ofsea louse infection pressure. In such cases, hierarchical(mixed effects) models can account for the non-independenceof responses while simultaneously drawing inferences frommany salmon populations (e.g. [26]).

Models can make predictions, but the definitive test for acausal influence of pathogens on host population dynamicsrequires experimental manipulation of disease in wild hostpopulations. This can be accomplished by experimentalreduction of pathogen burdens and the comparison of survi-val or population dynamics between treated and controlgroups. Such experiments have been carried out withAtlantic salmon, where thousands of smolts ( juvenilesalmon) were released in paired trials. Half of the fish weretreated with a parasiticide that provided temporary protec-tion from infection by sea lice in early marine life, while theother half received a control treatment. A meta-analysis of24 paired-release experiments (totalling 283 347 fish)showed that lack of treatment (a surrogate of parasiteexposure) decreased survival of returning adults by 39%[3]. Although there was variation among trials in effective-ness of treatments, with some trials showing no evidence ofeffect and others showing a strong effect, collective examin-ation revealed clear evidence of a positive effect of theparasiticide treatment on salmon recruitment (figure 5d ).

4. Management approachesDirect management of disease is more feasible in farmedsystems than in wild populations. As with terrestrial farms,management options range from chemical treatments, to gen-etic selection for disease resistance, to integrated pestmanagement, to regional coordination of fallowing andstocking. Sea lice management often occurs on a single

http://rstb.royalsocietypublishing.org/https://www.researchgate.net/publication/51601282_Effects_of_parasites_from_salmon_farms_on_productivity_of_wild_salmon?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/51601282_Effects_of_parasites_from_salmon_farms_on_productivity_of_wild_salmon?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/51601282_Effects_of_parasites_from_salmon_farms_on_productivity_of_wild_salmon?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/233602573_Sea_lice_Lepeophtheirus_salmonis_infestations_and_the_productivity_of_pink_salmon_Oncorhynchus_gorbuscha_in_the_Broughton_Archipelago_British_Columbia_Canada?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/227657873_Coho_salmon_productivity_in_relation_to_salmon_lice_from_infected_prey_and_salmon_farms?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/12852528_Spatial_and_temporal_variation_in_the_infestation_of_sea_trout_Salmo_trutta_L_by_the_Caligid_Copepod_Lepeophtheirus_salmonis_Kroyer_in_relation_to_sources_of_infection_in_Ireland?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/49675530_Relationship_of_farm_salmon_sea_lice_and_wild_salmon_populations?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/222477497_Reducing_Uncertainty_in_the_Biological_Basis_of_Fisheries_Management_by_Meta-Analysis_of_Data_from_Many_Populations_A_Synthesis?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/233332595_Impact_of_parasites_on_salmon_recruitment_in_the_Northeast_Atlantic_Ocean_Proc?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/236580147_Understanding_sources_of_sea_lice_for_salmon_farms_in_Chile?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/237054764_Cessation_of_a_salmon_decline_with_control_of_parasites?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/237054764_Cessation_of_a_salmon_decline_with_control_of_parasites?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==

Farm1

Farm2

Farm3

Farm1

Farm2

Farm3

l1

l2

l3

(a) (b)

Figure 4. Force of infection, l, exerted from surrounding farms j ¼ 1, 2, 3, on a point location (yellow triangle) can be calculated (a) using distance as a proxy,such that lj is larger for closer farms. In this example, l1 . l2 . l3. In panel (b), l is calculated more explicitly as per the water flow direction and speed (i.e.longer arrows represent faster flow). Even though farm1 is closest, it is not a likely source of infection given the direction of flow. Further, farm3 is located too faraway and the pathogen is not viable by the time it reaches that location of interest. Infection status of farms is shown as dark red (infected) and light green (notinfected), and of water carrying viable pathogens as dark red (infected) and light blue (non-viable). (Online version in colour.)


371:20150203

6


farm or across several farms within a region. On a farm,fallowing after harvest can interrupt sea louse populationgrowth, while treatments administered to fish can removeor kill attached sea lice [9,10]. Models have been useful fordeveloping management strategies at both levels. Delay-differential equation models have been used to show howtwo pulsed chemical treatments delivered six weeks apartcan be more effective than regular single treatments for con-trolling the abundance of sea lice [49], while individual-basedmodels that combine chemical treatments with othermethods of control (e.g. stocking cleanerfish that eat adultsea lice) are useful for optimizing integrated pest manage-ment strategies on farms [27]. Modelling, therefore, canhelp salmon farmers balance sustainable management prac-tices with economic viability. In particular, coupledbiological–economical models of sea louse treatmentapproaches can help the salmon farm industry conductmore responsive evidence-based management (e.g. [50]).

At a regional scale, generalized linear statistical modelsindicated that the prevalence of sea lice at the farm levelincreases with host abundance on that farm and proximityto other infected farms [34,35]. These results support a strat-egy of coordinated fallowing among farms at spatial andtemporal scales chosen to break infection cycling amongneighbouring farms. In addition, responsiveness to the con-nectivity and density among salmon farms, coordination oftreatment applications and agreements on treatmentthresholds can all help to regulate sea lice at a regional level.

Environmental factors, including water temperature,salinity and water movement, also influence the potential forsea louse epidemics. Modelling these factors might help withforecasting epidemics and siting and stocking salmon farms[51]. In Pacific Canada, a multivariable statistical model of a10-year dataset of sea louse parasitism on out-migratingchum and pink salmon was made to account for abiotic fac-tors, farm activity and spatial processes of parasite dispersaland fish migration [41]. This comprehensive approach is an

invaluable tool for evaluating how marine aquaculture andparasite management policies influence spatial patterns ofinfection levels on wild fish given environmental conditions[41]. Environmental effects on infections within salmonfarms have also been quantified using theoretical simulationmodels. For example, a modified susceptible-exposed-infec-tive-resistant ordinary differential equation model showedthat stronger currents and higher stocking levels increasedtransmission of sea lice between two hypothetical farms [52].A matrix model that explicitly accounted for demographic sto-chasticity among sea lice raised concerns about the impact ofrising sea surface temperatures on sea louse dynamics byshowing that both high temperatures and increased tempera-ture variation can increase the sea louse basic reproductionnumber, R0, and decrease generation time of sea lice, resultingin faster population growth [20]. The development of thesemathematical models that incorporated environmental impactshas been facilitated by numerous laboratory studies thatallowed for the parametrization of stage-specific effects oftemperature on sea louse development [53].

Attached sea lice tend to cluster at different spatial scales.This is an important consideration for the design of managementstrategies or monitoring programmes. As with many parasites,sea lice are often over-dispersed among hosts: a few fish mayexperience high-intensity infections while most have few or nosea lice [54]. At the farm scale, with multiple cages of salmon,sea lice aggregate more heavily in some cages than in others.At both host and farm scales, this spatial heterogeneity hasimplications for sea louse demography and monitoring [20].Monte Carlo simulations and matrix population modellingsuggest that when sea louse abundance is maintained below amean of three gravid females per host on farms, mate limitationdecreases the rate of sea louse reproduction [20,55]. However,over-dispersion of sea lice on hosts can increase mating successby bringing males and females into contact more frequently[20]. Over-dispersion may also have disadvantages for parasitefitness, because parasite-induced host mortality can have

http://rstb.royalsocietypublishing.org/https://www.researchgate.net/publication/12738603_Sealice_on_Salmonids_Their_Biology_and_Control?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/6868215_Ecology_of_sea_lice_parasitic_on_farmed_and_wild_fish?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/232809965_Use_of_agent-based_modelling_to_predict_benefits_of_cleaner_fish_in_controlling_sea_lice_Lepeophtheirus_salmonis_infestations_on_farmed_Atlantic_salmon_Salmo_salar_L?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/221818005_Sea_lice_as_a_density-dependent_constraint_to_salmonid_farming?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/263215171_Evaluating_the_effect_of_clustering_when_monitoring_the_abundance_of_sea_lice_populations_on_farmed_Atlantic_salmon?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/250218436_Population_dynamics_of_salmon_lice_Lepeophtheirus_salmonis_on_Atlantic_salmon_and_sea_trout?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/260127207_Modelling_the_Impact_of_Temperature-Induced_Life_History_Plasticity_and_Mate_Limitation_on_the_Epidemic_Potential_of_a_Marine_Ectoparasite?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/260127207_Modelling_the_Impact_of_Temperature-Induced_Life_History_Plasticity_and_Mate_Limitation_on_the_Epidemic_Potential_of_a_Marine_Ectoparasite?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/260127207_Modelling_the_Impact_of_Temperature-Induced_Life_History_Plasticity_and_Mate_Limitation_on_the_Epidemic_Potential_of_a_Marine_Ectoparasite?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/260127207_Modelling_the_Impact_of_Temperature-Induced_Life_History_Plasticity_and_Mate_Limitation_on_the_Epidemic_Potential_of_a_Marine_Ectoparasite?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/230840847_Modelling_salmon_lice_Lepeophtheirus_salmonis_reproduction_on_farmed_Atlantic_salmon_Salmo_salar_L?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/229414188_Assessing_topical_treatment_interventions_on_Scottish_salmon_farms_using_a_sea_lice_Lepeophtheirus_salmonis_population_model?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/236887686_Space-Time_Modelling_of_the_Spread_of_Salmon_Lice_between_and_within_Norwegian_Marine_Salmon_Farms?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/274382917_Spatial_patterns_of_sea_lice_infection_among_wild_and_captive_salmon_in_western_Canada?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/274382917_Spatial_patterns_of_sea_lice_infection_among_wild_and_captive_salmon_in_western_Canada?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/267984733_A_SYSTEM_DYNAMICS_APPROACH_TO_SEA_LICE_CONTROL_IN_NORWAY?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==

1 5 20 100odds ratio (1SW fish)

overall meta-analytic effect

individual trials

0 0.05 0.10 0.15 0.20

0

0.4

0.8

1.2

recr

uits

(00

0s)

spawners (000s)

–2

–1

0

1

2

3

4

popu

latio

n gr

owth

rat

e

pre-lice lice

unexposedexposed

fallow

period

0 2 4 6 8 10 12

–4

–2

0

2

4

farm lice (millions)

ln(r

ecru

its/s

paw

ner)

(a) (b)

(c) (d)

Figure 5. Stock – recruitment relationships evaluating the effect of sea lice on wild salmon population dynamics. (a) An example Ricker stock – recruitment relation-ship (the Kwalate coho population in British Columbia) illustrating the observed (grey circles) and predicted (black line) number of individuals in one generation(recruits) produced by spawners in the previous generation. Stock productivity is the maximum ratio of recruits to spawners and is the initial slope of the stock –recruitment curve [25]. (b) Coho salmon population growth rates (+95% bootstrapped CIs) from exposed (black circles) and unexposed regions (grey circles) priorto and during years when salmon lice epidemics were observed in exposed populations as well as during a fallow treatment year [25]. (c) Productivity (loge (recruitsper spawner)) of coho salmon populations in relation to the estimated number of lice on farmed fish during juvenile coho seaward migration. The black line is theestimated relationship between productivity and louse abundance based upon a hierarchical linear regression [26]. (d ) A random effect meta-analysis of chemother-apeutant treatment on the likelihood of adult Atlantic salmon returning to spawn. Lines are the 95% CIs of the effect size for each trial with the closed diamondrepresenting the overall meta-analytic effect across all studies [3].


371:20150203

7


disproportionate impacts on parasite population dynamicssuch that each mortality of a heavily infected host provides asubstantial reduction in the parasite population [16,54]. From amanagement perspective, heterogeneity in infection load canhamper estimation of sea louse abundance. Empirical studiesand mathematical simulations using individual-based modelssupport the idea that sampling a few fish from many pens,rather than sampling many fish from few pens, improves esti-mates of louse abundances at the host and farm levels [54].Because sea louse counts influence management decisions, accu-rate estimates of infection intensity are essential for optimizingtreatment regimens [56]. Therefore, the spatial distribution ofsea lice among hosts must be understood and accounted for inmonitoring programmes.

Aquaculture practices, particularly chemical treatments onsalmon farms, can place selection pressures on sea lice. Despitetheir implications for sustainable aquaculture, such evolu-tionary processes are poorly explored in marine diseasemanagement [57]. Those studies that exist demonstrate thataquaculture can cause substantial evolution of parasites. Forexample, microparasites evolved greater virulence whenfaced with elevated host densities on salmon farms (e.g. [53])and, in some areas, sea lice have evolved resistance to thechemicals used to control them [58,59]. Ordinary differentialequation models and individual-based models show that thefrequency of treatments and mixing rates between sea lice on

farmed and wild untreated hosts can influence the rate ofresistance evolution [60,61]. Mixing between wild-origin sealice and farm sea lice can dilute the proportion of resistant indi-viduals and provide an opportunity for resistant alleles to beremoved by natural selection if they have fitness costs [60].

5. Future directionsOver the past 30 years, substantial progress has been madetowards understanding how wild and farmed salmon popu-lations interact to influence infectious disease dynamics. Asaquaculture practices change and disease ecology matures asa multidisciplinary field, new applied and theoretical questionscontinue to emerge. We summarize some of these future direc-tions here.

(a) Co-ordinated management of multiple pathogensManaging the health of wild and farmed populationsextends beyond a single pathogen. When infection withone pathogen increases susceptibility to another (e.g. [62]),or when multiple pathogens may be transmitted throughthe same hydrodynamic pathways (e.g. [29]), coordinatingmanagement of multiple diseases may be advantageous.Coordinated efforts are most effective when they take intoaccount the impacts of disease on the health of the

http://rstb.royalsocietypublishing.org/https://www.researchgate.net/publication/230693640_Regulation_and_Stability_of_Host-Parasite_Population_Interactions_I_Regulatory_Processes?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/51601282_Effects_of_parasites_from_salmon_farms_on_productivity_of_wild_salmon?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/227657873_Coho_salmon_productivity_in_relation_to_salmon_lice_from_infected_prey_and_salmon_farms?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/227657873_Coho_salmon_productivity_in_relation_to_salmon_lice_from_infected_prey_and_salmon_farms?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/235773258_Transmission_dynamics_of_pancreas_disease_PD_in_a_Norwegian_fjord_Aspects_of_water_transport_contact_networks_and_infection_pressure_among_salmon_farms?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/263215171_Evaluating_the_effect_of_clustering_when_monitoring_the_abundance_of_sea_lice_populations_on_farmed_Atlantic_salmon?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/263215171_Evaluating_the_effect_of_clustering_when_monitoring_the_abundance_of_sea_lice_populations_on_farmed_Atlantic_salmon?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/250218436_Population_dynamics_of_salmon_lice_Lepeophtheirus_salmonis_on_Atlantic_salmon_and_sea_trout?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/5599810_The_Efficacy_of_Emamectin_Benzoate_against_Infestations_of_Lepeophtheirus_salmonis_on_Farmed_Atlantic_Salmon_Salmo_salar_L_in_Scotland_2002-2006?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/235369648_Detection_of_emamectin_benzoate_tolerance_emergence_in_different_life_stages_of_sea_lice_Lepeophtheirus_salmonis_on_farmed_Atlantic_salmon_Salmo_salar_L?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/241091431_A_simple_model_to_assess_selection_for_treatment-resistant_sea_lice?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/241091431_A_simple_model_to_assess_selection_for_treatment-resistant_sea_lice?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/233332595_Impact_of_parasites_on_salmon_recruitment_in_the_Northeast_Atlantic_Ocean_Proc?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/262772619_Applying_evolutionary_concepts_to_wildlife_disease_ecology_and_management?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/261742054_Disease_Resistance_in_Atlantic_Salmon_Salmo_salar_Coinfection_of_the_Intracellular_Bacterial_Pathogen_Piscirickettsia_salmonis_and_the_Sea_Louse_Caligus_rogercresseyi?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/230626798_Confidence_in_assessing_the_effectiveness_of_bath_treatments_for_the_control_of_sea_lice_on_Norwegian_salmon_farms?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/283017626_Using_Agent-Based_Modelling_to_Predict_the_Role_of_Wild_Refugia_in_the_Evolution_of_Resistance_of_Sea_Lice_to_Chemotherapeutants?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==


371:20150203

8


surrounding farms and wild communities and the role thatthe environment may play in mediating these impacts.

(b) Putting disease into a community contextInteractions between parasites and host communities arenumerous [63] and poorly understood for many marine patho-gens. Multi-host models are underutilized and could help toimprove our understanding of feedback between disease andcommunities. For example, in both the Pacific and AtlanticOceans, herring (Clupea sp.) form large potential host popu-lations for generalist sea lice (e.g. C. clemensi and C. elongatus)and differ in their migration timing from co-occurring salmon[64]. The extent to which herring, or other host species, play asignificant role in sea louse population dynamics, spread andevolution, is an important, unanswered question.

(c) Effects of aquaculture on evolutionary processesDespite the progress described above, the evolutionaryimplications of aquaculture—for hosts and for their para-sites—remain under-explored (but see [65]). The potentialselection pressures for increased defences in wild hosts andfor treatment resistance in pathogens are important con-siderations for disease management. For sea lice, ourunderstanding of disease-related evolutionary processes islimited to physiological changes in sea lice that make themmore tolerant of chemical treatments. However, sea louse be-haviour and life history could also undergo rapid selection.Finally, the role of artificial selection for disease-resistantfarmed hosts might also influence the dynamics of pathogenson both farmed and wild populations [61].

(d) Potential for better dataLack of data is a significant barrier to the development oftactical models of many marine diseases. While conceptualmodels can be parametrized using qualitative trends, site-or situation-specific models require detailed parametrization,and subsequent calibration and validation. In many cases, welack precise information on the population dynamics of wildpopulations and their infectious status. Conceptual modelsmight be useful for prioritizing data collection, such as byidentifying parameters with high model sensitivity. Ulti-mately, many management decisions depend upon morespecific, well-parametrized tactical models.

The salmon farming industry, government and conserva-tion groups often collect and hold complementary dataseparately. Some practical advances in data accessibility havealready been achieved through collaboration and data sharing.For example, in both Scotland and Atlantic Canada, evidenceof sea louse resistance to SLICEw was uncovered through mod-elling using shared industry-wide datasets well in advance oflaboratory-based confirmation [58,59]. Even in the relativelycontentious context of Pacific Canada, the Broughton Archipel-ago Monitoring Plan (www.bamp.ca) has illustrated thatprogress can be achieved through collaboration among stake-holders from industry, government and non-governmentalorganizations [15,41,66]. Keeping lines of communicationopen, while maintaining scientific integrity, will continue tobenefit management, science and conservation.

(e) Potential for better statistical modelsGreater computing power and more sophisticated statisticalmethods, now commonplace in ecology, will continue to

improve information gained from available data. Whereassumptions of simple dynamic patterns and normal errorspreviously prevailed, advanced Markov chain Monte Carlomethods now allow end-users to fit complex nonlinear,non-normal hierarchical statistical models. These methodsare well suited to the study of emerging infectious diseasesand we expect to see their use increase.

6. DiscussionMathematical models are an invaluable tool for understand-ing complex interactions in sea lice epidemiology. Their rolehas been to translate hypotheses into quantitative relation-ships between ecological processes, and they can yieldinsights into ecological dynamics. Consequently, models area quantitative framework with which to assess competingclaims; a tool to refine, clarify and communicate ideas; anda way to predict the outcomes of ecological or manage-ment-based changes. From interpretation of data todevelopment of novel theory, models have both facilitatedand driven inquiry.

While many biologists would predict some adverse effectsfrom maintaining elevated densities of host fish, feeding andprotecting hosts from predation, and placing farmed hosts incontact with wild migratory hosts, the details of these inter-actions have not always been clear. Only through thedevelopment of relevant hypotheses, translation into models,careful data collection and analysis—and the feedbackbetween them—has progress been possible.

Due in part to conflicts between economics and conserva-tion, there has been disagreement on the nature andmagnitude of effects of sea lice on wild populations[13,26,48,67,68]. One major challenge to research has beenthe inability to undertake ecosystem-wide controlledexperiments. This means that, by necessity, manipulativestudies are rare compared with correlative studies. Evenwhen manipulative studies have been possible [3,69], debateand controversy have persisted [70,71]. Another major chal-lenge is the fact that a negative statistical result does notnecessarily translate to a negative biological conclusion. Thissecond challenge arises because simple lack of evidence is notevidence of lack of an effect, particularly if the statistical powerof the approach is low. Fortunately, larger datasets increasestatistical power, and a spectrum of ecological conditionsgives the possibility of more closely emulating randomizedtrial conditions found in experimental manipulations.

As the aquaculture industry continues to expand andwild fish populations decline, new pathogens and noveltransmission pathways will emerge in both farmed andwild populations, raising similar questions to those askedabout sea lice over the past decades. What are the modes oftransmission? What is the potential for transmission betweenwild and farmed fish? If transmitted, how will these patho-gens affect individuals and populations? These questionsare not unique to salmon and sea lice, and it may be advan-tageous for scientists, industry and policy-makers toapproach other marine infectious diseases in light of all thathas been learned in this system. Indeed, in this review, wehave outlined only a fraction of the potential interactionsamong hosts, parasites and their environments. We hopethat questions surrounding features and outcomes illustratedhere will motivate further study of these complex systems.

http://www.bamp.cahttp://rstb.royalsocietypublishing.org/https://www.researchgate.net/publication/5769993_Declining_Wild_Salmon_Populations_in_Relation_to_Parasites_from_Farm_Salmon?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/51601282_Effects_of_parasites_from_salmon_farms_on_productivity_of_wild_salmon?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/49675530_Relationship_of_farm_salmon_sea_lice_and_wild_salmon_populations?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/5599810_The_Efficacy_of_Emamectin_Benzoate_against_Infestations_of_Lepeophtheirus_salmonis_on_Farmed_Atlantic_Salmon_Salmo_salar_L_in_Scotland_2002-2006?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/235369648_Detection_of_emamectin_benzoate_tolerance_emergence_in_different_life_stages_of_sea_lice_Lepeophtheirus_salmonis_on_farmed_Atlantic_salmon_Salmo_salar_L?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/280758304_Diverse_effects_of_parasites_in_ecosystems_Linking_interdependent_processes?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/234088087_Impact_of_Lepeophtheirus_salmonis_infestations_on_migrating_Atlantic_salmon_Salmo_salar_L_smolts_at_eight_locations_in_Ireland_with_an_analysis_of_lice-induced_marine_mortality?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/23676887_Comment_on_Declining_Wild_Salmon_Populations_in_Relation_to_Parasites_from_Farm_Salmon?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/233332595_Impact_of_parasites_on_salmon_recruitment_in_the_Northeast_Atlantic_Ocean_Proc?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/261107222_Comment_on_Jackson_et_al_'Impact_of_Lepeophtheirus_salmonis_infestations_on_migrating_Atlantic_salmon_Salmo_salar_L_smolts_at_eight_locations_in_Ireland_with_an_analysis_of_lice-induced_marine_mortali?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/250924474_Sea_lice_infestations_on_juvenile_chum_and_pink_salmon_in_the_Broughton_Archipelago_Canada_from_2003_to_2012?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/221853232_Life_history_and_virulence_are_linked_in_the_ectoparasitic_salmon_louse_Lepeophtheirus_salmonis?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/236193716_Modeling_Parasite_Dynamics_on_Farmed_Salmon_for_Precautionary_Conservation_Management_of_Wild_Salmon?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/274382917_Spatial_patterns_of_sea_lice_infection_among_wild_and_captive_salmon_in_western_Canada?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/283017626_Using_Agent-Based_Modelling_to_Predict_the_Role_of_Wild_Refugia_in_the_Evolution_of_Resistance_of_Sea_Lice_to_Chemotherapeutants?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==

rstb.royalsociety

9


Authors’ contributions. All authors contributed substantially to the con-ception and writing of this article.Competing interests. We have no competing interestsFunding. We acknowledge funding from the following sources: CERCin aquatic epidemiology (M.L.G., E.E.R.); Hakai Doctoral Scholarshipand NSERC CGS (L.A.R.); Watershed Watch Salmon Society andNSERC IPS (S.C.G.); NSERC Vanier CGS (S.J.P.); NSERC PDF

(A.W.B.); CRC (M.A.L., C.W.R.), NSERC Discovery and AcceleratorGrants (M.A.L.) and the Killam Foundation (M.A.L.).Acknowledgements. The authors thank Salmon Coast Field Station forhosting a formative meeting during the early development of themanuscript. Mike Foreman provided useful feedback relating tohydrodynamic modelling. William Chalmers and anonymousreviewers provided thoughtful editorial comments.

publishing.or
References gPhil.Trans.R.Soc.B
371:20150203

1. Wood CL, Lafferty KD, Micheli F. 2010 Fishing outmarine parasites? Impacts of fishing on rates ofparasitism in the ocean. Ecol. Lett. 13, 761 – 775.(doi:10.1111/j.1461-0248.2010.01467.x)

2. Ben-Horin T, Lafferty KD, Bidegain G, Lenihan HS.2016 Fishing diseased abalone to promote yield andconservation. Phil. Trans. R. Soc. B 371, 20150211.(doi:10.1098/rstb.2015.0211)

3. Krkošek M, Revie CW, Gargan PG, Skilbrei OT,Finstad B, Todd CD. 2013 Impact of parasites onsalmon recruitment in the Northeast Atlantic Ocean.Proc. R. Soc. B 280, 20122359. (doi:10.1098/rspb.2012.2359)

4. Harvell D et al. 2004 The rising tide of oceandiseases: unsolved problems and research priorities.Front. Ecol. Environ. 2, 375 – 382. (doi:10.1890/1540-9295(2004)002[0375:TRTOOD]2.0.CO;2)

5. Krkošek M et al. 2011 Fish farms, parasites, andpredators: implications for salmon populationdynamics. Ecol. Appl. 21, 897 – 914. (doi:10.1890/09-1861.1)

6. Godwin S, Dill L, Reynolds J, Krkošek M. 2015 Sealice, sockeye salmon, and foraging competition:lousy fish are lousy competitors. Can. J. Fish. Aquat.Sci. 72, 1113 – 1120. (doi:10.1139/cjfas-2014-0284)

7. Altizer S, Bartel R, Han BA. 2011 Animal migrationand infectious disease risk. Science 331, 296 – 302.(doi:10.1126/science.1194694)

8. Johns S, Shaw A. 2015 Theoretical insight into threedisease-related benefits of migration. Popul. Ecol.58, 213 – 221. (doi:10.1007/s10144-015-0518-x)

9. Pike A, Wadsworth S. 1999 Sealice on salmonids:their biology and control. Adv. Parasitol. 44,233 – 337. (doi:10.1016/S0065-308X(08)60233-X)

10. Costello MJ. 2006 Ecology of sea lice parasitic onfarmed and wild fish. Trends Parasitol. 22,475 – 483. (doi:10.1016/j.pt.2006.08.006)

11. Krkos̆ek M. 2010 Sea lice and salmon in PacificCanada: ecology and policy. Front. Ecol. Environ. 8,201 – 209. (doi:10.1890/080097)

12. Costello MJ. 2009 The global economic cost of sea liceto the salmonid farming industry. J. Fish Dis. 32,115 – 118. (doi:10.1111/j.1365-2761.2008.01011.x)

13. Krkošek M, Ford J, Morton A, Lele S, Myers RA,Lewis MA. 2007 Declining wild salmonpopulations in relation to parasites from farmsalmon. Science 318, 1772 – 1775. (doi:10.1126/science.1148744)

14. Ricker WE. 1954 Stock and recruitment. Fish. Res.Board Can. 11, 559 – 623. (doi:10.1139/f54-039)

15. Rogers LA, Peacock SJ, McKenzie P, DeDominicis S,Jones SRM, Chandler P, Foreman MGG, Revie CW,

Krkošek M. 2013 Modeling parasite dynamics onfarmed salmon for precautionary conservationmanagement of wild salmon. PLoS ONE 8, e60096.(doi:10.1371/journal.pone.0060096)

16. Anderson RM, May RM. 1978 Regulation andstability of host-parasite population interactions:I. Regulatory processes. J. Anim. Ecol. 47, 219 – 247.(doi:10.2307/3933)

17. Revie CW, Robbins C, Gettinby G, Kelly L, TreasurerJW. 2005 A mathematical model of the growth ofsea lice, Lepeophtheirus salmonis, populations onfarmed Atlantic salmon, Salmo salar L., in Scotlandand its use in the assessment of treatmentstrategies. J. Fish Dis. 28, 603 – 613. (doi:10.1111/j.1365-2761.2005.00665.x)

18. Peacock SJ, Connors BM, Krkošek M, Irvine JR, LewisMA. 2014 Can reduced predation offset negativeeffects of sea louse parasites on chum salmon?Proc. R. Soc. B 281, 20132913. (doi:10.1098/rspb.2013.2913)

19. Krkošek M, Lewis MA, Volpe JP. 2005 Transmissiondynamics of parasitic sea lice from farm to wildsalmon. Proc. R. Soc. B 272, 689 – 696. (doi:10.1098/rspb.2004.3027)

20. Groner ML, Gettinby G, Stormoen M, Revie CW,Cox R. 2014 Modelling the impact of temperature-induced life history plasticity and mate limitation onthe epidemic potential of a marine ectoparasite. PLoSONE 9, e88465. (doi:10.1371/journal.pone.0088465)

21. Revie CW, Gettinby G, Treasurer JW, Wallace C. 2003Identifying epidemiological factors affecting sea liceLepeophtheirus salmonis abundance on Scottishsalmon farms using general linear models. Dis.Aquat. Organ. 57, 85 – 95. (doi:10.3354/dao057085)

22. Middlemas SJ, Fryer RJ, Tulett D, Armstrong JD.2013 Relationship between sea lice levels on seatrout and fish farm activity in western Scotland.Fish. Manag. Ecol. 20, 68 – 74. (doi:10.1111/fme.12010)

23. Connors BM, Juarez-Colunga E, Dill LM. 2008 Effectsof varying salinities on Lepeophtheirus salmonissurvival on juvenile pink and chum salmon. J. FishBiol. 72, 1825 – 1830. (doi:10.1111/j.1095-8649.2008.01839.x)

24. Krkošek M, Hilborn R. 2011 Sea lice (Lepeophtheirussalmonis) infestations and the productivity ofpink salmon (Oncorhynchus gorbuscha) in theBroughton Archipelago, British Columbia, Canada.Can. J. Fish. Aquat. Sci. 68, 17 – 29. (doi:10.1139/F10-137)

25. Connors BM, Krkošek M, Ford J, Dill LM. 2010Coho salmon productivity in relation to salmon

lice from infected prey and salmon farms. J. Appl. Ecol.47, 1372– 1377. (doi:10.1111/j.1365-2664.2010.01889.x)

26. Krkošek M, Connors BM, Morton A, Lewis MA,Dill LM, Hilborn R. 2011 Effects of parasites fromsalmon farms on productivity of wild salmon. Proc.Natl Acad. Sci. USA 108, 14 700 – 14 704. (doi:10.1073/pnas.1101845108)

27. Groner ML, Cox R, Gettinby G, Revie CW. 2013 Useof agent-based modelling to predict benefits ofcleaner fish in controlling sea lice, Lepeophtheirussalmonis, infestations on farmed Atlantic salmon,Salmo salar L. J. Fish Dis. 36, 195 – 208. (doi:10.1111/jfd.12017)

28. Stucchi DJ, Guo M, Foreman MGG, Czajko P,Galbraith M, Mackas DDL, Gillibrand PA. 2011Modeling sea lice production and concentrationsin the Broughton Archipelago, British Columbia.In Salmon lice: an integrated approach tounderstanding parasite abundance and distribution(eds S Jones, R Beamish), pp. 117 – 150. Chichester,UK: Wiley-Blackwell.

29. Stene A, Viljugrein H, Yndestad H, Tavornpanich S,Skjerve E. 2014 Transmission dynamics of pancreasdisease (PD) in a Norwegian fjord: aspects of watertransport, contact networks and infection pressureamong salmon farms. J. Fish Dis. 37, 123 – 134.(doi:10.1111/jfd.12090)

30. Arneberg P, Skorping A, Grenfell B, Read AF. 1998Host densities as determinants of abundance inparasite communities. Proc. R. Soc. Lond. B 265,1283 – 1289. (doi:10.1098/rspb.1998.0431)

31. Anderson RM, May RM. 1979 Population biology ofinfectious diseases: part I. Nature 280, 361 – 367.(doi:10.1038/280361a0)

32. Lloyd-Smith JO, Cross PC, Briggs CJ, Daugherty M,Getz WM, Latto J, Sanchez MS, Smith AB, Swei A.2005 Should we expect population thresholds forwildlife disease? Trends Ecol. Evol. 20, 511 – 519.(doi:10.1016/j.tree.2005.07.004)

33. Krkošek M. 2010 Host density thresholds anddisease control for fisheries and aquaculture. Aquac.Environ. Interact. 1, 21 – 32. (doi:10.3354/aei0004)

34. Jansen PA, Kristoffersen AB, Viljugrein H, Jimenez D,Aldrin M, Stien A. 2012 Sea lice as a density-dependentconstraint to salmonid farming. Proc. R. Soc. B 279,2330 – 2338. (doi:10.1098/rspb.2012.0084)

35. Aldrin M, Storvik B, Kristoffersen AB, Jansen PA.2013 Space-time modelling of the spread of salmonlice between and within Norwegian marine salmonfarms. PLoS ONE 8, e64039. (doi:10.1371/journal.pone.0064039)

http://dx.doi.org/10.1111/j.1461-0248.2010.01467.xhttp://dx.doi.org/10.1098/rstb.2015.0211http://dx.doi.org/10.1098/rspb.2012.2359http://dx.doi.org/10.1098/rspb.2012.2359http://dx.doi.org/10.1890/1540-9295(2004)002[0375:TRTOOD]2.0.CO;2http://dx.doi.org/10.1890/1540-9295(2004)002[0375:TRTOOD]2.0.CO;2http://dx.doi.org/10.1890/09-1861.1http://dx.doi.org/10.1890/09-1861.1http://dx.doi.org/10.1139/cjfas-2014-0284http://dx.doi.org/10.1126/science.1194694http://dx.doi.org/10.1007/s10144-015-0518-xhttp://dx.doi.org/10.1016/S0065-308X(08)60233-Xhttp://dx.doi.org/10.1016/j.pt.2006.08.006http://dx.doi.org/10.1890/080097http://dx.doi.org/10.1111/j.1365-2761.2008.01011.xhttp://dx.doi.org/10.1126/science.1148744http://dx.doi.org/10.1126/science.1148744http://dx.doi.org/10.1139/f54-039http://dx.doi.org/10.1371/journal.pone.0060096http://dx.doi.org/10.2307/3933http://dx.doi.org/10.1111/j.1365-2761.2005.00665.xhttp://dx.doi.org/10.1111/j.1365-2761.2005.00665.xhttp://dx.doi.org/10.1098/rspb.2013.2913http://dx.doi.org/10.1098/rspb.2013.2913http://dx.doi.org/10.1098/rspb.2004.3027http://dx.doi.org/10.1098/rspb.2004.3027http://dx.doi.org/10.1371/journal.pone.0088465http://dx.doi.org/10.3354/dao057085http://dx.doi.org/10.1111/fme.12010http://dx.doi.org/10.1111/fme.12010http://dx.doi.org/10.1111/j.1095-8649.2008.01839.xhttp://dx.doi.org/10.1111/j.1095-8649.2008.01839.xhttp://dx.doi.org/10.1139/F10-137http://dx.doi.org/10.1139/F10-137http://dx.doi.org/10.1111/j.1365-2664.2010.01889.xhttp://dx.doi.org/10.1111/j.1365-2664.2010.01889.xhttp://dx.doi.org/10.1073/pnas.1101845108http://dx.doi.org/10.1073/pnas.1101845108http://dx.doi.org/10.1111/jfd.12017http://dx.doi.org/10.1111/jfd.12017http://dx.doi.org/10.1111/jfd.12090http://dx.doi.org/10.1098/rspb.1998.0431http://dx.doi.org/10.1038/280361a0http://dx.doi.org/10.1016/j.tree.2005.07.004http://dx.doi.org/10.3354/aei0004http://dx.doi.org/10.1098/rspb.2012.0084http://dx.doi.org/10.1371/journal.pone.0064039http://dx.doi.org/10.1371/journal.pone.0064039http://rstb.royalsocietypublishing.org/https://www.researchgate.net/publication/49771944_Animal_Migration_and_Infectious_Disease_Risk?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/49771944_Animal_Migration_and_Infectious_Disease_Risk?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/49771944_Animal_Migration_and_Infectious_Disease_Risk?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/43297938_Fishing_out_marine_parasites_Impacts_of_fishing_on_rates_of_parasitism_in_the_ocean?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/43297938_Fishing_out_marine_parasites_Impacts_of_fishing_on_rates_of_parasitism_in_the_ocean?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/43297938_Fishing_out_marine_parasites_Impacts_of_fishing_on_rates_of_parasitism_in_the_ocean?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/43297938_Fishing_out_marine_parasites_Impacts_of_fishing_on_rates_of_parasitism_in_the_ocean?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/294596236_Fishing_diseased_abalone_to_promote_yield_and_conservation?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/294596236_Fishing_diseased_abalone_to_promote_yield_and_conservation?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/294596236_Fishing_diseased_abalone_to_promote_yield_and_conservation?el=1_x_8&enrichId=rgreq-e2df90d94e48f7bcf8a7045fe1cd28dc-XXX&enrichSource=Y292ZXJQYWdlOzI5NDcyODcyMTtBUzozMzIwMjIxMTY1NjkwODhAMTQ1NjE3MTY0OTQxNg==https://www.researchgate.net/publication/294596236_Fishing_diseased_abalone_to_promote

Documents

Lessons from sea louse and salmon epidemiologymlewis/Publications 2016/Groner et...Figure 1. Data and models are key components in the scientific process. Although data and models