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Chromatin Nucleotides Dynamics Lecture
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Global Local at promoters of genes
Same nucleus
ChIPexperiments
Differential
Taverna et al. 2007
chromodomain
chromodomain
Dominant negative
Taverna et al. 2007
Histone/chromatin crosstalk
appears in mitosis but not interphase.
appears in mitosis but not interphase.
Chromatin / Nucleosome dynamics
Nucleosome folding /packaging regulatesaccessibility of DNA templates (nucleosomal DNA and
linker DNA)
The dynamics of chromatin structure are regulatedthrough multiple mechanisms including:
--histone variant incorporation--histone posttranslational modifications (PTMs)
--ATP-dependent chromatin remodeling --histone chaperones--architectural chromosomal proteins
Tessarz and Kouzarides (2014)
Cell cycle regulated
* *
Results
Conclusions
Jack et al (2013)
Tessarz and Kouzarides (2014)
Lysine
Tessarz and Kouzarides (2014)
Nucleosome lateral surfacemodifications
-may modulate histone-DNAinteractions --> nuc stability --> nuc position stability ->increased mobility =increased accessibility
PTMs may affectnucleosome structures and
dynamics
Tessarz and Kouzarides (2014)
Nucleosomehistone-histonemodifications
--may destabilizetetramer-dimerinteractions --> nucstability --> histoneexchange
Tessarz and Kouzarides (2014)
Nucleosome facemodifications
--may modulatechromatin-bindingprotein interactionspositively / negatively
--may modulate nuc-nuc interactions -->higher order chromatinstructure
+ respective writers, erasers, readers
+ EFFECTORS (dynamic interactions)