Lab safety (eng) - Kasetsart University

Lab Safety Rules and Guidelines

Content

** General lab safety rules

** Safety Symbols and Hazard Signs, Meanings

** Emergency procedureg y p

** Safety data sheet (SDS)

Dress code safety rules

Always do !

Y h ld l l b t

Tie back hair that is chin-length or longer.

You should always wear lab coat.

Before leaving the lab or eating, Before leaving the lab or eating,

always wash your hands.

Footwear should always cover the foot completely.

Laboratory rules

* Do not work alone in the lab

Always don’t !

Do not work alone in the lab.

* Never leave an ongoing experiment unattended.

* Do not chew gum, drink, or eat while working in the lab.g , , g

* Do not smoking

* Do not wear the gloves and lab coat outside laboratory

* Pets and children are prohibited

* Never lift any glassware, solutions, or other types of apparatus above eye level.

Chemicals

Chemical safety rulesChemical safety rules* Every chemical should be treated as though it were dangerous.

* All chemicals should always be clearly labeled with the name of the substance, y y ,

its concentration, the date it was received, and the name of the person responsible

for it.

* Before removing any of the contents from a chemical bottle, read the label twice.

* Do not put unused chemicals back into their original container.

* Ch i l th t i l h ld b t k t f th l b t* Chemicals or other materials should never be taken out of the laboratory.

* Chemicals should never be mixed in sink drains.

* Flammable and volatile chemicals should only be used in a fume hood. Flammable and volatile chemicals should only be used in a fume hood.

* Ensure that all chemical waste is disposed of properly.

* Water should not be poured into concentrated acid. Instead, pour acid slowly into

water while stirring constantly. In many cases, mixing acid with water is

exothermic.

Chemicals

(Globally Harmonised System for Classification and Labeling of Chemicals GHS(Globally Harmonised System for Classification and Labeling of Chemicals, GHS

Label Type Purpose Typical Label Location Example

NFPA 704 Di d P id i f ti b t O t id b ildi dNFPA 704 Diamond

(National Fire

Protection

Provides information about

hazards that occur during

emergency response

Outside buildings, on doors,

on tanks, visible to

emergency responders

Association) during spill or fire

DOT placard

(Department of


hazards to transportation

Tank cars, cargo tanks,

portable tanks bulk (Department of

Transportation)

hazards to transportation

workers and emergency

responders

portable tanks, bulk

packages, vehicles of

containers containing non-

bulk packages

OSHA Hazcom 2012

(Occupational Safety


hazards to worker using

Pipes, drums and

containers of materials that (Occupational Safety

and Health

Administration)

hazards to worker using

chemicals under normal

conditions of use

containers of materials that

are used in the workplace

Chemicals


* Unstable explosives

* Explosives, Self-reactive substances and mixtures

* Organic peroxides

ExplosiveExplosive

Chemicals


* Flammable substances

* Self-heating substances

and mixtures

Flammable

* Substances and mixtures,

which in contact with water, Flammable ,

emit flammable gases

* Organic peroxidesg p

Chemicals


* Oxidizing gases Oxidizing gases

* Oxidizing liquids

* Oxidizing solids

Oxidizing agent

Oxidizing solids

g g

Chemicals


* Compressed gases Compressed gases

* Liquefied gases

Gas under pressurep

Chemicals


* Corrosive to metals/skin Corrosive to metals/skin

Corrosive

Chemicals


* Acute toxicity

(oral, dermal, inhalation)

Toxic

* Acute hazards to the aquatic environment

Dangerous to the environment

Chemicals


* Respiratory sensitization

* Germ cell mutagenicity

* Carcinogenicity

Health hazards

* Reproductive toxicity

* Specific target organ toxicity following single/repeated g g y g g

exposure

* Aspiration hazard

Chemicals


* Acute toxicity (oral, dermal, inhalation)

* Skin irritation

* Eye irritation

Caution / Harmful

* Skin sensitization

* Specific target organ toxicity following single g g y g g

exposure

Chemicals

GHS: NFPAGHS: NFPA

Emergency procedure

Chemical Spill ManagementChemical Spill Management

Consult the Material Safety Data Sheet, but generally the following applies:

Organic Material use vermiculate to absorb and place spent vermiculite in

labelled container for waste disposal labelled container for waste disposal.

Acid spills should be neutralised with sodium bicarbonate. Laboratory Spill Kits p y p

usually contain soda ash (sodium bicarbonate) to sprinkle liberally over the spill.

Avoid breathing soda ash dust.

Alkali spills can be neutralised with boric acid.

First Aid for Chemical Exposures

How long should the skin or eyes be flushed with water in the event of a

chemical exposure?

* 5-minutes for non-irritants or mild irritants

* 15-20 minutes for moderate to severe irritants and chemicals that cause acute toxicity if

absorbed through the skin

* 30 minutes for most corrosives

* 60 minutes for strong alkalis (e.g., sodium, potassium or calcium hydroxide)


When should vomiting be induced following ingestion of a chemical?

Vomiting should NOT be induced following ingestion of a chemical in an

occupational setting unless advised by a Poison Centre or doctor.

This following information will assist them in determining the best course of action.

* the name of the product swallowed

* the amount swallowed

* the condition of the person

Should water or milk be given to dilute a chemical that has been ingested?

Based on their evaluation of the evidence for dilution with milk or water, the American

Heart Association and American Red Cross recommend that people should not take

anything by mouth for an ingested poison unless specifically told to do so by a doctor anything by mouth for an ingested poison unless specifically told to do so by a doctor

or the Poison Control Centre.


How do I know which antidote to have available for the chemicals in my workplace?

It is a common misperception that antidotes are available for most chemical poisonings.

* Activated charcoal is sometimes considered to be an antidote Activated charcoal Activated charcoal is sometimes considered to be an antidote. Activated charcoal

works by binding the chemical in the stomach so it cannot be absorbed through the

stomach (which is known to be adsorbed to charcoal) up to 1 hour previously.

Some chemical classes do have true antidotes;

* Consulting with a doctor

* The manufacturer/supplier of your product

Emergency cell

Important number

B k fi i T l 02 21039Bangkaen fire station Tel. 02-5210397

Paolo hospital Tel. 02-150-0900

Bangkaen police station Tel 02-5212232 02-5210070Bangkaen police station Tel. 02 5212232, 02 5210070

Dr. Pichit Somboon Tel. (+66) 84-3886249

Dr. Pratuang Puthsom Tel. (+66) 95-5425055

Dr. Trairat Neimsuwan Tel. (+66) 89-4773504

Theerat Ardsamang Tel. (+66) 86-5207006

Safety Data Sheet (SDS)

1 ขอ้มลเกีย่วกบัสารเคม ีบรษิทัผผ้ลติและหรอืจาํหน่าย (identification)1. ขอมลูเกยวกบสารเคม บรษทผผูลตและหรอจาหนาย (identification)

2. ขอ้มลูความเป็นอนัตราย (hazards identification)

3. สว่นประกอบและขอ้มลูเกีย่วกบัสว่นผสม (composition/information on ingredients)

4. มาตรการปฐมพยาบาล (first aid measures)

5. มาตรการผจญเพลงิ (fire fighting measures)

6. มาตรการจดัการเมือ่มกีารหกรัว่ไหล (accidental release measures) ( )

7. การใชแ้ละการจดัเกบ็ (handling and storage)

8. การควบคมุการไดร้บัสมัผสัและการป้องกนัสว่นบุคคล (exposure controls/personal protection)

9 สมบตัทิางกายภาพและเคมี (physical and chemical properties) 9. สมบตทางกายภาพและเคม (physical and chemical properties)

10. ความเสถยีรและการเกดิปฏกิริยิา (stability and reactivity

11. ขอ้มลูดา้นพษิวทิยา (toxicological information)

12. ขอ้มลูดา้นระบบนิเวศ (ecological information)

13. ขอ้พจิารณาในการกาํจดั (disposal considerations)

14. ขอ้มลูสาํหรบัการขนสง่ (transport information) ู ( p )

15. ขอ้มลูเกีย่วกบักฎขอ้บงัคบั (regulatory information)

16. ขอ้มลูอืน่ๆ (other information)

Laboratory introduction

Water systemWater system

* นํ้าปราศจากไอออน (Deionized water; DI)

D i i ti (DI) d i li ti i h i f f d t i Deionization (DI), demineralization or ion exchange removes ions from feed water using

synthetic resins.

Usage: Regular experiment, chemical preparation, Second rinse glassware.

* นํ้ากลัน่ (distilled water, DW)

Perhaps the best known method of water purification is distillation in which water is heated to

the boiling pointthe boiling point.

Usage: Biological experiment, chemical preparation.

* นํ้าประปา (Tab water)

Contains many substances that if left untreated may react

or catalyze reactions in undesired ways;

inorganic ions organic molecules bacteria virusesinorganic ions, organic molecules, bacteria, viruses

Usage: First rinse glassware.

แนะนําอปุกรณ์ในห้องปฏิบตัิการ

Drainage systemDrainage system

Hazardous wastes are prohibited from sink/drain disposal.

* Corrosive Solutions (pH at/or below 5.5 or at/above 12.0)

* Solutions that are flammable (flash point less than 60oC) Solutions that are flammable (flash point less than 60 C)

* Volatile organic compounds (acetone, alcohol, etc.)

* Any solid (glass, wood chips, pulp, etc.)

* Heavy metals solution

Lab Safety Rules and Guidelines

“The way to love anything is to realize

that it may be lost.”

“หนทางทีจ่ะรกับางสิง่คอืการคดิไดว้า่มนั

ไป”จะหายไป”― Gilbert K. Chesterton

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Lab safety (eng) - Kasetsart University