lab 02 microscope

8/7/2019 lab 02 microscope

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02

Diffusion, Microscope &Cell Lab


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Diffusion experimentMethylene blue MW 320g/mole

Potassium permagnate MW 150 g/mole

Form Hypothesis

T0 initial diameter T60 final diameter

Activity 1

Room temp Fridge


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A B

Carrying a Microscope


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Return the lowest power

objective in place

Wrap the cord around the

base

Return dustcover

Storing The Microscope


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Use lens paper on all glass parts of the

microscope.

Clean oil immersion lens with chemicalsprovided by your instructor

Cleaning the Microscope


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Parts of the Microscope

Fine adjustment knob

Stage

Stage clips

Aperture

Diaphragm

Light

Base

Ocular

Body tube

Nosepiece

Objective lenses

(10, 40, 100x)

Arm

Course

adjustment knob


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Microscope Parts

A. OcularB. Body tube

C. Stage clip

D. Revolving nose piece

E. Objective

F. Arm

G. Stage

H. Diaphragm

I. Lever to move stage clip

J. Course adjustmentK. Fine adjustment

L. Light source

M. Base


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Using the microscope

Always observe using the LOWEST POWER objective

first.

Focus using the COARSE ADJUSTMENT KNOB to

bring the object into focus. Bring the object into sharp

focus by using the fine adjustment knob. Focus, and then move to a higher power objective, if

needed.

Use only the FINE ADJUSTMENT KNOB when using

the HIGHEST (longest) POWEROBJECTIVE.

Keep both eyes open to reduce eyestrain.

Determine total magnification of the object by

multiplying the power of the ocular (10x) the power by

the power of the objective.


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Preparing a slide

Using a pipet or dropper, add a drop of water or

another solvent to a clean microscope slide.

Then, place the specimen in the water.

Place the edge of a coverslip on the slide so that

it touches the edge of the water. Slowly lower the coverslip to prevent the

formation of air bubbles.


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40x 100x

Oil Immersion Lens


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AnimalCell


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Plant Cell


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Prepare wet mount Cheek cell

Elodea cell

Onion cell

Look at specimen under high power

and draw what you see.

Use proper clean-up technique

Activity 2


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Cheek Cell


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Elodea


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EpidermalOnion Cell


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Prepare wet mount for dissecting scope Pond water

Look at specimen under high power and

draw what you see.Use proper clean-up technique

Activity 3


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Dispose Biological material


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Dispose Sharps


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Clean Up


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Microscope Parts Quiz


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Microscope Quiz

1.

1. Which objective uses oil?2. If your ocular is 10x and your

objective is 40x what is the total

magnification of your image?3. Why do you need a cover slip and

how do you avoid an air bubble?

4. What is the difference between a

light microscope and a dissectingmicroscope?


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Materials & Equipment 5 dissecting scopes

5 light microscopes

Cover slips

Slides (box)

Droppers Water in dropper

Oil and cleaner for oil immersion

lens

Lens paper

Onion

Elodea

Toothpicks (box)

Sharps and biohazard bag

disinfectant

Instructors Notes:

5 Rulers

10 agar plates plus a

few extra

Methylene blue

Potassium permagnate

Razor blade to cut

plants

Pondwater

Use of fridge

Documents

lab 02 microscope