Curriculum Vitae Amnon Kohen

Department of Chemistry Tel: (319) 335-0234 University of Iowa FAX: (319) 335-1270 Iowa City, IA 52242 amnon-kohen@uiowa.edu EDUCATION AND PROFESSIONAL HISTORY Education D.Sc., Chemistry 1989-1994 Technion – Israel Institute of Technology, Haifa, Israel Advisor: Professor T. Baasov Topic: Mechanistic Studies of the Enzyme KDO8P Synthase

B.Sc., Chemistry (with Honors) 1986-1989 Hebrew University, Jerusalem, Israel Positions Professor 2010-Present Department of Chemistry, University of Iowa, Iowa City, IA and Molecular and Cellular Biology Program, and MSTP faculty member Associate Professor 2005-2010 Department of Chemistry, University of Iowa, Iowa City, IA and Molecular and Cellular Biology Program

Assistant Professor 1999-2005 Department of Chemistry, University of Iowa, Iowa City, IA Postgraduate Researcher 1997-1999 With Professor Judith Klinman Department of Chemistry, University of California, Berkeley

Topic: Hydrogen Tunneling in Biology: Alcohol Dehydrogenases Postgraduate Fellow 1995-1997 With Professor Judith Klinman Department of Chemistry, University of California, Berkeley Topic: Hydrogen Tunneling in Biology: Glucose Oxidase Visiting Scholar Fall 1994 With Professor Karen S. Anderson Department of Pharmacology, Yale Medical School, New Haven, CT

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Affiliations American Society for Biochemistry and Molecular Biology (ASBMB, 2013 - present) Center for Biocatalysis and Bioprocessing (CBB) University of Iowa (2000-Present)

The Interdisciplinary Graduate Program in Molecular Biology, University of Iowa (2003-Present) American Association for the Advancement of Science (AAAS, 2011-present) American Chemical Society (1995-Present) Divisions: Organic Chemistry, Physical Chemistry, Biochemistry Sigma Xi (1997-Present) Protein Society (1996-1998)

Honors and Awards

• The Lady Davis Visiting Professorship (The Lady Davis Fellowship Trust, Israel – 2009) • Faculty Scholars Award (University of Iowa- 2007-2010) • Career Development Award (University of Iowa- 2006-2007) • NSF Faculty Early Career Development (CAREER) Award (2002-2007) • Wolf Foundation Award for excellence in doctoral studies (1992) • Miriam and Aaron Gutwirth Award for excellence in graduate studies (1991) • Amos-De-Shalit Scholarship for distinguished undergraduate students (1987) • Hebrew University Dean’s List (1987-1989)

SCHOLARSHIP

Publications All the publication below are in a peer reviewed journals and books. (* = Corresponding author, senior author, major contribution)

Publications resulting from work performed at Iowa:

1. Abeysinghe, T., Hong, B., Wang, Z., Kohen, A.,* “Preserved Hydride Transfer Mechanism in Evolutionary Divergent Thymidylate Synthases”, Submitted to J. Biol. Chem., Oct. 2014.

2. Singh, P., Morris, H., Tivanski, A.V., Kohen, A.,* “Determination of concentration and activity of immobilized enzymes”, Submitted to Anal., Biochem., Oct. 2014.

3. Francis, K., Kohen, A.,* “The Temperature Dependence of the Intrinsic Kinetic Isotope Effects of Human Dihydrofolate Reductase”, Submitted to Chem. Commun. Sept. 2014.

4. Mishanina, T., Kohen, A.,* “Synthesis and application of isotopically labeled flavin nucleotides”, Submitted to J. Labeled Comp. Radiopharma., Sept. 2014.

5. Doron, D., Stojković, V., Gakhar, L., Kohen, A., Major, D.,* “Free Energy Simulations of Active-Site Mutants of Dihydrofolate Reductase”, Submitted to J. Phys. Chem. June 17, 2014.

6. Kohen, A.,* “Role of Dynamics in Enzyme Catalysis: Substantial vs. Semantic Controversies”, Acc. Chem. Res. (Invited Review for Special Issue on “Enzymes Dynamics and Catalysis”) In Press, 2014. http://dx.doi.org/10.1021/ar500322s

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7. Liu, C.T.,‡ Francis, K.,‡ Layfield , J., Huang, X., Hammes-Schiffer, S., Kohen, A.,* Benkovic, S.J.,* “The Escherichia coli Dihydrofolate Reductase Catalyzed Proton and Hydride Transfers: Order and the Roles of Asp27 and Tyr100”, Proc. Nat., Acad. Sci. USA, In Press, 2014.

8. Klinman, J.P.,* Kohen, A,* “Evolutionary Aspects of Enzyme Dynamics”, J. Biol. Chem., Published on line Sept. 10, 2014. (an invited review; to be published in the Nov. issue)

9. Mishanina, T., Corcoran, J.M., Kohen, A.,* “Substrate Activation in Flavin-Dependent Thymidylate Synthase”, J. Am. Chem. Soc., 2014, 136, 10597–10600.

10. Roston, D., Kohen, A., Doron, D., Major, D.T.,* “Simulations of Remote Mutants of Dihydrofolate Reductase Reveal the Nature of a Network of Residues Coupled to Hydride Transfer”, J. Comp. Chem., 2014, 35, 1411-1417.

11. Islam, Z., Strutzenberg, S.T., Gurevic, I., and Kohen, A.*, “Concerted versus Step-Wise Mechanism in Thymidylate Synthase”, J. Am. Chem. Soc. 2014,136, 9850–9853.

12. Wang, Z., Singh, P., Czekster, C., Kohen, A.,* Schramm, V.L.,* “Protein Mass-modulated Effects in the Catalytic Mechanism of Dihydrofolate Reductase: Beyond Promoting Vibrations”, J. Am. Chem. Soc., 2014, 136, 8333-8341.

13. Doron, D., Kohen, A., Nam, K., Major, D.T.,* “How Accurate are Transition States from Simulations of Enzymatic Reactions?” J. Chem. Theory Comput., Accepted for Publication March 31, 2014.

14. Francis, K., Kohen, A.,* “Protein Motions and the Activation of the C-H Bond Catalyzed by Dihydrofolate Reductase”, Curr. Opinion Chem. Biol., 2014, 21, 19–24. (Invited Review).

15. Singh, P., Sen, A., Francis, K., Kohen, A.,* “Extension and limits of the network of coupled motions correlated to hydride transfer in dihydrofolate reductase”, J. Am. Chem. Soc., 2014, 136, 2575–2582.

16. Roston, D., Kohen, A.,* “Stereospecific Multiple Isotopic Labeling of Benzyl Alcohol”, J. Labeled

Comp. Radiopharma., 2014, 57, 75-77.

17. Roston, D., Ialam, Z., Kohen, A., “Kinetic Isotope Effects as a Probe of Hydrogen Transfers to and from

Common Enzymatic Cofactors”, Archives Biochem. Biophys, 2014, 544, 96-104.

18. Francis, K., Kohen, A., “Standards for the Reporting of Kinetic Isotope Effects in Enzymology”,

Perspectives in Science, 2014, 1, 110–120. A STRENDA and Beilstein book, Eds. Cornish-Bowden, A.,

and Cattenr, C., Pub. Elsevir. (Invited Chapter).

19. Francis, K., Stojković, V., Kohen, A.,* “Preservation of Protein Dynamics in Enzyme Evolution“, J. Biol.

Chem., 2013, 288, 35961-35968.

20. Roston, D., Kohen, A.,* “A Critical Test of the “Tunneling and Coupled Motion” Concept in Enzymatic

Alcohol Oxidation”, J. Am. Chem. Soc., 2013, 135, 13624–13627.

21. Islam, Z, Roston, D., Kohen, A., “Isotope effects as probes for H-tunneling and enzyme dynamics”, Special Issue on "Isotope Effects" in Molecules, Ed. Hansen, P.E., 2013, 18, 5543-5567. (Invited Review).

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22. Wang, Z., Sapienza, P.J., Abeysinghe, T., Luzum, C., Lee, A.L., Finer-Moore, J.S., Stroud, R.M., and Kohen, A.,* “Mg2+ binds to the surface of thymidylate synthase and affects hydride transfer at the interior active site”, J. Am. Chem. Soc., 2013. 135, 7583-7592.

23. Wang., Z., Ferrer, S., Moliner, V.,* Kohen, A.,* “QM/MM calculations suggest a unique mechanism for the proton abstraction catalyzed by thymidylate synthase”, Biochemistry, 2013, 52, 2348-2358.

24. Kohen, A, “Positron emission tomograpgy tracer“, US 2013/0034496 A1, Patent application published Feb. 7, 2013.

25. Cheatum, C.M.,* Kohen, A,* “Relationship of Femtoseconds Dynamics to the Catalyzed Hydrogen Transfer” Topics in Current Chemistry, Special issue on “Macromolecular Dynamics in Biological Catalysis”, Eds, Hammes-Schiffer, S. & Klinman, J.P., Pub: Elsevier, (Invited Review). Published on-line March 28, 2013.

26. Kohen, A.*, Klinman, J.P.*, “Hydrogen Tunneling Links Protein Dynamics to Enzyme Catalysis”, Ann. Rev. Biochem., 2013, 82, 471–496. (Invited Review).

27. Wang, Z., Abeysinghe, T., Finer-Moore, J.S., Stroud, R.M., Kohen, A.*, “A Remote Mutation Affects the Hydride Transfer by Disrupting Concerted Protein Motions in Thymidylate Synthase”, J. Am. Chem. Soc., 2012, 134, 17722-17730.

28. Vardi-Kilshtain, A., Major, D.T.,* Kohen, A., Engel, H., Doron, D., “Hybrid Quantum and Classical Simulations of the Formate Dehydrogenase Catalyzed Hydride Transfer Reaction on an Accurate Semi-Empirical Potential Energy Surface”, J. Chem. Theory Comput., 2012, 8, 4786–4796.

29. Sen, A., Stojković, V., Kohen, A.,* “ Synthesis of radiolabeled nicotinamide cofactors from labeled pyridines: versatile probes for enzyme kinetics“, Anal. Biochem. 2012, 430, 123–129.

30. Roston, D., Cheatum, C.M., Kohen, A.,* “Hydrogen Donor-Acceptor Fluctuations from Kinetic Isotope Effects: A Phenomenological Model”, Biochemistry, 2012, 51, 6860–6870.

31. Liu, Q., Zhao, Y., Hammann, B., Eilers, J., Lu, Y.,* Kohen, A.,* “A Model Reaction Assesses Contribution of H-Tunneling and Coupled Motions to Enzyme Catalysis”, J. Org. Chem., 2012, 77, 6825−6833.

32. Koehn, E.M., Perissinotti, L.L., Moghram, S., Prabhakar, A., Lesley, S.A., Mathews, I.I.,* Kohen, A.,* “The folate binding site of flavin-dependent thymidylate synthase”, Proc. Nat. Acad. Sci. USA, 2012, 109, 15722-15727.

33. Kohen, A., “Enzyme Dynamics: Consensus and Controversy”, J. Biocatal. Biotransformation 2012, 1:1, doi: 10.4172/jbcbt.1000e103 (Editorial).

34. Doron, D., Kohen, A., Major, D.T.,* “Collective Reaction Coordinate for Hybrid Quantum and Molecular Mechanics Simulations: A Case Study of the Hydride Transfer in Dihydrofolate Reductase”, J. Chem. Theory Comput., 2012, 8, 2484–2496.

35. Engel, H., Doron, D. Kohen, A., Major, D.T.,* “Momentum Distribution as a Fingerprint of Quantum Delocalization in Enzymatic Reactions: Open-Chain Path-Integral Simulations of Model Systems and the Hydride Transfer in Dihydrofolate Reductase”, J. Chem. Theory Comput., 2012, 8, 1223–1234.

36. Mishanina, T.V., Koehn, E.M., Conrad, J.A., Palfey, B., Lesley, S.A., Kohen, A.*, “Flavin-Dependent Thymidylate Synthase: Trapping of a Reaction Intermediate”, J. Am. Chem. Soc., 2012, 134, 4442–4448.

37. Wang, Z., Roston, D., Kohen, A.*, “Experimental and theoretical studies of enzyme-catalyzed hydrogen transfer reactions”, in Structural and Mechanistic Enzymology: Bringing together Experiments and

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Computing, Vol. Eds. Christov, C.Z., and Karabencheva-Christova, T., in series entitled Advance in Protein Chemistry and Structural Biology, Ed. Donev, R., Burlington, Academic Press, Elsevier Inc, Vol. 87, pp. 155-180, 2012. ISBN: 978-0-12-398312-1 (invited chapter).

38. Stojković, V., Perissinotti, L.L., Willmer D.J., Benkovic, S.J., Kohen, A.*, “Effects of the donor acceptor distance and dynamics on hydride tunneling in the dihydrofolate reductase catalyzed reaction”, J. Am. Chem. Soc., 2012, 134, 1738-1745.

39. Saeed, M., Tewson, T.J., Erdahl, C.E., Kohen, A.*, “A fast chemo-enzymatic synthesis of [11C]-N5,N10-methylenetetrahydrofolate as a potential PET tracer for proliferating cells”, Nucl. Med. Biol., 2012, 39, 697-701 (http://www.nucmedbio.com/article/S0969-8051(11)00305-2/abstract).

40. Mishanina, T.V., Koehn, E., Kohen, A.*, “Mechanisms and inhibition of uracil methylating enzymes”, Bioorg. Chem., 2012, 43, 37–43 (http://www.sciencedirect.com/science/article/pii/S0045206811000952) (invited review).

41. Dutta, S., Li, Y-L., Rock, W., Houtman, J.C., Kohen, A., Cheatum, C.M.*, "3-Picolyl Azide Adenine Dinucleotide as a Probe of Femtosecond to Picosecond Enzyme Dynamics", J. Phys. Chem. B., 2012, 116, 542–548.

42. Doron, D. Major, D.T.,* Kohen, A., Thiel, W., Wu, X., “Hybrid Quantum and Classical Simulations of the Dihydrofolate Reductase Catalyzed Hydride Transfer Reaction on an Accurate Semi-Empirical Potential Energy Surface”, J. Chem. Theory Comput., 2011, 7, 3420-3437.

43. Oyeyemi, O.A., Sours, K.M. Lee, T., Kohen, A., Resing, K.A., Ahn, N.G., and Klinman, J.P.*, “Comparative Hydrogen-Deuterium Exchange for a Mesophilic vs. Thermophilic Dihydrofolate Reductase at 25ºC: Identification of a Single Active Site Region with Enhanced Flexibility in the Mesophilic Protein”, Biochemistry 2011, 50, 8251–8260.

44. Saeed, M., Sheff, D, Kohen, A.*, “A novel positron emission tomography tracer distinguishes normal from cancerous cells ”, J. Biol. Chem., 2011, 286, 33872–33878.

45. Ditzler, L., Sen, A., Gannon, M., Kohen, A.,* Tivanski, A.,* “Self-assembled enzymatic monolayer directly bound to a gold surface: Activity and molecular recognition force spectroscopy studies”, J. Am. Chem. Soc. (Communication) 2011, 133, 13284-13287.

46. Dutta, S., Rock, W., Cook, R.J., Kohen, A., and Cheatum, C.M.*, “Two-dimensional infrared spectroscopy of azido-nicotinamide adenine dinucleotide in water”, J. Phys. Chem. published online Aug. 5, 2011, 135, 055106; (6 pages) doi:10.1063/1.3623418.

47. Sen, A., Yahasiri, A., Kohen, A.*, “Triple isotopic labeling and kinetic isotope effects: a sensitive and accurate method for exposing H-transfer steps in enzymatic systems”, Biochemistry, 2011, 50, 6462–6468. This article has been chosen to be highlighted on the journal’s home page.

48. Kanaan, N., Ferrer, S., Martí, S, Garcia-Viloca, M., Kohen, A., Moliner, V.*, “Temperature Dependence of the Kinetic Isotope Effects in Thymidylate Synthase. A Theoretical Study”, J. Am. Chem. Soc., 2011, 133, 6692-6702.

49. Kohen, A.*, Roston, D., Stojković, V., Wang, Z. In Encyclopedia of Analytical Chemistry; Meyers, R. A., Ed.; John Wiley & Sons, Ltd: Chichester, UK, 2011; Vol. S1-S3, p 77-99, ISBN: (978-0-470-97333-2).

50. Stojković, V., Perissinotti, L.L., Lee, J., Benkovic, S.J., Kohen, A.*, “The effect of active-site isoleucine to alanine mutation on the DHFR catalyzed H-transfer”, Chem. Commun. 2010, 46, 8974 - 8976.

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51. Dutta, S., Cook, R.J., Houtman, J.C.D., Kohen, A.*, and Cheatum, C.M.*, “Characterization of azido-NAD+ to assess its potential as a two-dimensional infrared probe of enzyme dynamics”, Anal. Biochem., 2010, 407, 241–246.

52. Bandaria, J., Dutta, S., Nydegger, M.W., Rock, W., Kohen, A., and Cheatum, C.M.*, “Characterizing the dynamics of functionally relevant complexes of formate dehydrogenase”, Proc. Natl. Acad. Sci. USA. 2010, 107, 17974–17979.

53. Wang, Z., and Kohen, A.*, “Thymidylate synthase catalyzed H-transfers: Two chapters in one tale”, J. Am. Chem. Soc., 2010, 132, 9820–9825.

54. Roston, D., and Kohen. A.*, “The elusive transition state of alcohol dehydrogenase unveiled”, Proc. Natl. Acad. Sci. USA., 2010, 107, 9572-9577.

55. Sen, A., and Kohen, A.*, “Enzymatic tunneling and kinetic isotope effects: chemistry at the crossroads”, J. Phys. Org. Chem. 2010, 23, 613–619 (invited review for a special issue entitled “Symposium in Print on Tunneling”).

56. Koehn, E.M., Kohen, A.*, “Flavin-dependent thymidylate synthase: A novel pathway towards thymine”, Archiv. Biochem. Biophys. 2010, 493, 96 – 102.

57. Yahashiri, A.; Nimrod, G.; Ben-Tal, N.; Howell, E.E.; Kohen, A.*, “Effect of electrostatic shielding on H-tunneling in R67 dihydrofolate”, ChemBioChem, 2009, 10, 2620 - 2623.

58. Hill, S.E., Bandaria, J.N., Fox, M., Vanderah, E., Kohen, A., and Cheatum, C.M.*, “Exploring the Molecular Origins of Protein Dynamics in the Active Site of Human Carbonic Anhydrase II”, J. Phys Chem. B. 2009, 113, 11505 - 11510.

59. Yahashiri, A., Sen, A., and Kohen, A.*, “Microscale synthesis and kinetic isotope effect analysis of (4R)-[Ad-14C, 4-2H] NADPH and (4R)-[Ad-3H,4-2H] NADPH”, J. Labeled Comp. Radiopharma, 2009, 52 463–466.

60. Bandaria, J., Cheatum, C.M., and Kohen, A.*, “Examination of enzymatic H-tunneling through kinetics and dynamics”, J. Am. Chem. Soc., 2009, 131, 10151–10155.

61. Koehn, E.M.,; Fleischmann, T., Conrad, J.A., Palfey, B., Lesley, S.A.; Kohen, A.*, “An unusual mechanism of thymidylate biosynthesis in organisms containing the thyX gene” Nature 2009, 458 919-923.

62. Wang, Z., Chernyshev, A., Koehn, E.M., Manuel, A., Lesley, S.A., and Kohen, A.*, “Oxidase Activity of a Flavin-Dependent Thymidylate Synthase”, FEBS J., 2009, 276, 2801–2810.

63. Stojković, V.; Kohen A.*, “Enzymatic H-transfers: Quantum Tunneling and Coupled Motion from Kinetic Isotope Effect Studies”, Isr. J. Chem. 2009, 49, 163-173 (Invited Review).

64. Sen, A., and Kohen, A.*, “Quantum Effects in Enzyme Kinetics”, in Quantum Tunneling in Enzyme Catalyzed Reactions, Allemann, R. and Scrutton, N., Eds, Royal Society of Chemistry, London, UK, 2009, Ch. 7, pp.161-178. ISBN 978-0-85404-122-0

65. Kanaan, N.; Marti, S.*; Moliner, V.*; Kohen, A., “QM/MM study of Thymidylate Synthase: Enzymatic motions and the temperature dependence of the rate-limiting step", J. Phys. Chem. A. 2009, 113, 2176–2182 (Special Issue on Chemical Dynamics: Max Wolfsberg Festschrift, Sharon Hammes-Schiffer Ed.).

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66. Kohen A., Spotlight: “Deep Tunneling Dominates the Biologically Important Hydride Transfer Reaction from NADH to FMN in Morphinone Reductase” J. Am. Chem. Soc. 2008, (invited Spotlight on JACS 2008, 130, 7092, http://pubs.acs.org/JACSbeta/jvi/issue3.html).

67. Yahashiri, A.; Howell, E.E.; Kohen, A.*, “Tuning of the H-Transfer Coordinate in Primitive vs. Well-Evolved Enzymes”, ChemPhysChem 2008, 9, 980-982.

68. Bandaria, J., Dutta, S., Hill, S., Kohen, A., and Cheatum, C.*, “Fast Enzyme Dynamics at the Active Site of Formate Dehydrogenase”, J. Am. Chem. Soc. 2008, 130, 22-23 (Communication).

69. Hong, B., Maley, F, and Kohen, A.*, “Role of Y94 in Proton and Hydride Transfers Catalyzed by Thymidylate Synthase”, Biochemistry., 2007, 46, 14188-14197.

70. Chernyshev, A., Fleischmann, T., Koehn, E., Lesley, S.A., and Kohen, A.*, “The relationships between oxidase and synthase activities of flavin-dependent thymidylate synthase (FDTS)”, Chem. Commun. 2007, 2861 - 2863.

71. Kanaan, N., Martí, S.*, Moliner, V.*, and Kohen, A., “A QM/MM Study of the catalytic mechanism of the thymidylate synthase”, Biochemistry 2007, 46, 3704-3713.

72. Chernyshev, A., Fleischmann, T., and Kohen, A.*, “Enzymes of thymidyl biosynthesis as antibiotic targets”,

Appl. Microbiol. Biotech. 2007, 74, 282-289 (invited review). 73. Kohen, A.*, “Coupled motion and dynamics in enzyme catalysis”, in Hydrogen-Transfer Reactions, Hynes, J.T.,

Klinman, J.P., Limbach, H.H., Schowen, R.L. Eds., Wiley VCH, Weinheim, 2007, Vol. 4, Ch. 12, pp. 1311-1340 [invited chapter].

74. Kohen, A.*, “Kinetic isotope effects as probes for hydrogen tunneling in enzyme catalysis” in Isotope Effects in

Chemistry and Biology, Kohen, A. and Limbach, H.H. Eds., Taylor & Francis -CRC Press, Boca Raton, FL, 2006, Ch. 28, pp-743-764. ISBN 0824724496 [invited chapter]

75. Wang, L., Goodey, N.M., Benkovic, S.J.*, and Kohen, A.*, "Coordinated Effects of Distal Mutations on

Environmentally Coupled Tunneling in Dihydrofolate Reductase", Proc. Natl. Acad. Sci. USA 2006, 103, 15753–15758.

76. Markham, K.A., and Kohen, A.*, “Analytical Procedures for the Preparation, Isolation, Analysis and Preservation

of Reduced Nicotinamides”, Curr. Anal. Chem., 2006, 2, 379-388 (invited review). 77. Hong, B., Haddad, M., Maley, F., Jensen, J.H., and Kohen, A.*, “Hydride transfer versus

hydrogen radical transfer in thymidylate synthase”, J. Am. Chem. Soc. (communication) 2006, 128, 5636 – 5637.

78. Mason, A., Agrawal, N., Washington, T., Lesley, S.A., and Kohen, A.*, “A lag-phase in the reduction of flavin-

dependent thymidylate synthase (FDTS) revealed a mechanistic missing link”, Chem. Commun. 2006, 1781 - 1783.

79. Limbach, H.H.*, Lopez, J.M., and Kohen, A., “Arrhenius curves of hydrogen transfers: Tunnel effects, isotope

effects and effects of pre-equilibria”, Phil. Trans. R. Soc. B, 2006, 361,1399 - 1415.

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80. Wang, L., Goodey, N.M., Benkovic, S.J., and Kohen, A.*, “The role of enzyme dynamics and tunneling in catalyzing hydride transfer: studies of distal mutations of dihydrofolate reductase”, Phil. Trans. R. Soc. B, 2006, 361, 1307-1215.

81. Wang, L., Tharp, S., Seltzer, T., Benkovic, S.J., and Kohen, A.*, “Effects of a distal mutation on active site

chemistry”, Biochemistry, 2006, 45, 1383-1392. 82. Pu, J., Ma, S., Garcia-Viloca, M., Gao, J.,* Truhlar, D.G.,* and Kohen, A., “Nonperfect synchronization of

reaction center rehybridization in the transition sate of the hydride transfer catalyzed by dihydrofolate reductase”, J. Am. Chem. Soc. 2005, 127, 14789-14886.

83. Hong, B. and Kohen, A.*, “Microscale synthesis of isotopically labeled 6R-N5, N10 methylene-5, 6, 7, 8-

tetrahydrofolate”, J. Labeled Comp. Radiopharma. 2005, 48, 759-769. 84. Sra, A.K., Hu, Y. Martin, G.E., Snow, D.D., Ribbe, M.W., and Kohen, A.*, “Competitive 15N Kinetic Isotope

Effects of Nitrogenase Catalyzed Dinitrogen Reduction”, J. Am. Chem. Soc. 2004, 126, 12768-12769. 85. Agrawal, N., Lesley, S., Kuhn, and Kohen, A.*, “Mechanistic Studies of a Flavin-Dependent Thymidylate

Synthase”, Biochemistry (Accelerated Publication), 2004, 43, 10295-10301. 86. Sikorski, R. S., Wang, L., Markham, K. A., Rajagopalan, P. T. R., Benkovic, S. J., and Kohen, A.*, “Tunneling

and Coupled Motion in the E. coli Dihydrofolate Reductase Catalysis”, J. Am. Chem. Soc. 2004, 126, 4778-4779. 87. Agrawal, N., Hong, B., Mihai, C., and Kohen, A.*, “Vibrationally Enhanced Hydrogen Tunneling in the E. coli

Thymidylate Synthase Catalyzed Reaction”, Biochemistry 2004, 43, 1998-2006. 88. Markham, K.A., Sikorski, R.S. and Kohen, A.*, “Synthesis and utility of 14C-labeled nicotinamide cofactors”,

Anal. Biochem. 2004, 325, 62–67. 89. Agrawal, N., Mihai, C., and Kohen, A.*, “Microscale synthesis of isotopically labeled (R)-[6- XH]N5, N10-

methylene-5,6,7,8-tetrahydrofolate as a cofactor for thymidylate synthase”, Anal. Biochem. 2004, 328, 44-50. 90. McCracken J.A., Wang, L. and Kohen, A.*, “Synthesis of R and S tritiated reduced nicotinamide adenine

dinucleotide 2` phosphate”, Anal. Biochem. 2004, 324, 131-136. 91. Markham, K.A., Sikorski, R.S. and Kohen, A.*, “Purification, analysis, and preservation of reduced nicotinamide

adenine dinucleotide 2’-phosphate”, Anal. Biochem. 2003, 322, 26-32. 92. Agrawal, N. and Kohen, A.*, “Microscale Synthesis of 2-tritiated isopropanol and 4R-tritiated reduced

nicotinamide adenine dinucleotide phosphate”, Anal. Biochem. 2003, 322, 179-184. 93. Kohen, A.*, “Kinetic isotope effects as probes for hydrogen tunneling, coupled motion and dynamics

contributions to enzyme catalysis”, Prog. React. Kin. Mech. 2003, 28, 119-156. [Review] 94. Kohen, A.* and Jensen J.H., “Boundary Conditions for the Swain-Schaad Relationship as a Criterion for

Hydrogen Tunneling”, J. Am. Chem. Soc. 2002, 124, 3858-3864. 95. Sra, A.K. and Kohen, A.*, “Nitrogenase catalyzed dinitrogen reduction – a new mechanistic approach” In

Nitrogen fixation Global Perspectives; Finan, T. M., O'Brian, M. R., Layzell, D. B., Vessey, J. K., Newton, W., Eds.; CABI Publishing: Oxon, New York; 2002, p 371.

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96. Truhlar, D. G.* and Kohen, A.*, “Convex Arrhenius plots and their interpretation”, Proc. Natl. Acad. Sci. USA

2001, 98, 848-851.

Book Edited

Isotope Effects in Chemistry and Biology. Kohen, A. and Limbach, H.H. Eds., Taylor & Francis - CRC Press, Boca Raton, FL, 2006, ISBN: 0824724496.

Manuscripts in Preparation

1. Nilaweera, T.D., Saeed, M., Kohen, A.,* “Enhancement of methylentetrahydrofolate uptake by inhibition of its endogenious biosynthesis ”, In preparation for JBC.

2. Abeysinghe, T., Kohen, A.,* “The role of Long-Range Protein Dynamics in Thymidylate Synthase Catalyzed Reactions and Altered Kinetic Steps of Y209W Mutant”, In preparation for J Am. Chem. Soc.

3. Mishanina, T., Kohen, A.,* “Mechanistic investigation of flavin-dependent thymidylate synthase”, In preparation for PNAS.

4. Roston, D., Dvir, D., Kohen, A.,* Major, D.T.,* “Simulations of Remote Mutants of Dihydrofolate Reductase Reveal the Nature of a Network of Residues Coupled to Hydride Transfer”, In preparation for J. Phys., Chem.

5. Hammann, B., Liu, Q.,; Bradshaw, J., Hall, C., Eilers, J., Lu, Y.,* Kohen, A., "Comparison of the Temperature-Dependence of Deuteride Kinetic Isotope Effects in Enzymes vs. Solution", in Preparation for Org. Lett.

6. Yahasiri, A., Kohen, A.*, “Dihydrofolate reductase catalyzed reduction of dihydrobiopterin: The role of the tail”, In preparation for Biochemistry.

7. Roston, D., Hong, B., Mihai, C., and Kohen, A.*, “2˚ Kinetic isotope effect studies with thymidylate synthase as a probe for combined methylene-hydride transfer”, in preparation for Biochemistry.

Press Exposure

1. “UI researcher studies evolution on the molecular level”, by Gary Galluzzo, http://now.uiowa.edu/2013/12/ui-researcher-studies-evolution-molecular-level Iowa New, Dec. 13, 2013.

2. Wang., Z., Ferrer, S., Moliner, V.,* Kohen, A.,* “QM/MM calculations suggest a unique mechanism for the proton abstraction catalyzed by thymidylate synthase”, Biochemistry, ASAP, 2013. http://pubs.acs.org/journals/bichaw/index.html highlighted on the journal’s home page

3. A fast chemoenzymatic synthesis of [11C]-N5,N10-methylenetetrahydrofolate as a potential PET tracer for proliferating cells, Global Medical Discovery [ISSN 1929-8536], Jan. 21, 2013.

4. UI chemists publish findings on how enzyme motion affects chemical reactions, University of Iowa News Release, Sept. 29, 2010.

5. Biochemistry: Anchors away, Maria Paola Costi & Stefania Ferrari, Nature 2009, 458, 840-841. A Nature “News and Views” covering our Nature paper in the same issue (Nature 2009, 458, 919-923).

6. Partial list of press releases related to our Nature 2009, 458, 919-923:

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a. UI chemists' DNA biosynthesis discovery could lead to better antibiotics, University of Iowa News Release, Apr. 15, 2009.

b. Scientists discover new chemical reaction for DNA production in bacteria and viruses, National Science Foundation News, Press Release 09-072, Apr. 16, 2009.

c. New Chemical Reaction For DNA Production In Bacteria And Viruses Has Potential For Development Of New Antibacterial And Antiviral Drugs, Medical News Today, Apr. 18, 2009 (linked from Medical New Today and MediLexicon).

d. An Unusual Mechanism for the Antimicrobial Target Flavin-dependent Thymidylate Synthase (FDTS), Stanford Synchrotron Radiation Lightsource (scientific highlight), Apr. 27, 2009.

e. Antibiotic target Nature Structural Genomics PSI-SGKB [doi:10.1038/fa_psisgkb.2009.33] Research Advances http://kb.psi-structuralgenomics.org/update/2009/08/full/fa_psisgkb.2009.33.html, July 16, 2009.

7. Enzymes' Many Movements, C&EN 2009, 87 (17), 34-36. An article covering a symposium Kohen and Warshel co-organized at the Spring 2009 ACS national meeting in Salt Lake City Utah.

8. Chemical Biology, Issue 8, 2007, presents: “The relationships between oxidase and synthase activities of flavin-dependent thymidylate synthase (FDTS), Anatoly Chernyshev, Todd Fleischmann, Eric M. Koehn, Scott A. Lesley and Amnon Kohen, Chem. Commun., 2007, 2861-2863”.

9. Warfield, R., “FAD stage down to the dumps: Exploring the mechanism of a thymidylate synthase”, Chemical

Biology, 2006, vol. 1, page B19, Royal Society of Chemistry Publishing (on-line Apr. 16, at www.rsc.org/ChemBiology).

10. Nigel S. Scrutton, Michael J. Sutcliffe, and P. Leslie Dutton, ”Quantum catalysis in enzymes: beyond the

transition state theory paradigm. A Discussion Meeting held at the Royal Society on 14 and 15 November 2005” J. Royal. Chem. Soc. Interface, doi:10.1098/rsif.2006.0122, published on-line March 21, 2006.

11. Borman S., “Much Ado About Enzyme Mechanisms”, C&E News 2004, Feb. 23, 35-39. 12. Wilson E.K., “Enzyme Dynamics”, C&E News 2000, July 17, 42-45.

Publications resulting from work performed prior to the appointment at Iowa

1. Kohen, A. and Klinman, J.P.*, “Protein flexibility correlate with degree of hydrogen tunneling in thermophilic and mesophilic alcohol dehydrogenase”, J. Am. Chem. Soc. 2000, 122, 10738-10739.

2. Kohen, A. and Klinman, J.P.*, “Hydrogen tunneling in biology”, Chemistry & Biology 1999, 6, R191-198. 3. Kohen, A., Cannio, R., Bartolucci, S., and Klinman, J.P.*, “Enzyme dynamics and hydrogen tunneling in a

thermophilic alcohol dehydrogenase”, Nature 1999, 399, 496-499. 4. Liang, P.H., Lewis, J., and Anderson, K.S., Kohen, A., D'Souza, F.W., Benenson, Y., and Baasov,T.*

“Catalytic mechanism of KDO8P synthase: transient kinetic studies and evaluation of a putative reaction intermediate”, Biochemistry 1998, 37, 16390-16399.

5. Kohen, A., Cannio, R., Bartolucci, S., and Klinman, J.P.*, “Beyond the classics: Hydrogen tunneling in enzyme

reaction”, Biophys. J. 1998, 74, A117-A117.

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6. Kohen, A. and Klinman, J.P.*, “Enzyme catalysis: beyond classical paradigms”, Acc. Chem. Res. 1998, 31, 397-404.

7. Kohen, A., Cannio, R., Bartolucci, S., and Klinman, J.P.*, “Thermophilic dehydrogenase: Hydrogen tunneling

at high and low temperature”, FASEB J. 1997, 11, A1134-A1134. 8. Liang, P.H., Kohen, A., Baasov, T., and Anderson, K.S.*, “Catalytic mechanism of KDO8P synthase. Pre-

steady-state kinetic analysis using rapid chemical quench flow methods”, Bioorganic & Medical Chem. Lett. 1997, 7, 2463-2468.

9. Kohen, A., Jonsson, T., and Klinman, J.P.*, “Effects of protein glycosylation on catalysis: Changes in hydrogen

tunneling and enthalpy of activation in the glucose oxidase reaction”, Biochemistry 1997, 36, 2603-2611. 10. Kohen, A., Jonsson, T., and Klinman, J.P.*, “Effect of enzyme glycosylation on the chemical step of catalysis,

as probed by hydrogen tunneling and enthalpy of activation”, Tech. Prot. Chem. VIII, D. R. Marshak Ed., 1997, pp. 311-319.

11. Baasov, T.* and Kohen, A. “Synthesis, inhibition and acid-catalyzed hydrolysis studies of model compounds of

the proposed intermediate in the KDO8P synthase catalyzed reaction”, J. Am. Chem. Soc. 1995, 117, 6165-6174.

12. Kohen, A., Belakhov, V., and Baasov, T.*, “Towards the synthesis of the putative reaction intermediate in the

KDO8P synthase - catalyzed reaction. Synthesis and evaluation of 3-deoxy-D-manno-octulosonate-2-phosphate”, Tetrahedron. Lett. 1994, 35, 3179-3182.

13. Baasov, T.*, Sheffer-Dee-Noor, S., Kohen, A., Jakob, A., and Belakhov, V. “Catalytic mechanism of 3-deoxy-

D-manno-octulosonate-8-phosphate synthase. The use of synthetic analogues to probe the structure of the putative reaction intermediate”, Eur. J. Biochem. 1993, 217, 991 – 999.

14. Kohen, A., Berkovich, R., Belakhov, V., and Baasov, T.*, “Stereochemistry of the KDO8P synthase. An

efficient synthesis of the 3-fluoro analogues of KDO8P”, Bioorganic & Medical Chem. Lett. 1993, 3, 1577-1582.

15. Kohen, A., Jakob, A., and Baasov, T.*, “Mechanistic studies of 3-deoxy-D-manno-octulosonate-8-phosphate

synthase from Escherichia coli”, Eur. J. Biochem. 1992, 208, 443-449.

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Funding Sources

ACTIVE GRANTS:

NIH-RO1 (continuously renewed since since 2002) Role: PI Sponsor: National Institutes of Health (NIGMS – RO1) Title: Tunneling and dynamics in enzyme catalyzed reactions (PI: Kohen) Amount: $1,441,890 Duration: June 2014 – Feb. 2018 NIH-RO1 Role: PI Sponsor: National Institutes of Health (NIGMS) Title: Catalytic Mechanism of Alternative Thymidylate Synthase (PI: Kohen)

Amount: $ 1,162,700.00 Duration: Aug. 2014 – July 2018 NSF (3rd renewal of the 2002 CAREER AWARD) Role: PI Sponsor: National Science Foundation (CHE – Experimental Physical Chemistry) Title: Protein dynamics and hydrogen tunneling in enzymatic catalysis (PI: Kohen)

Amount: $400,000 Duration: June 2012 - May. 2015

NSF (Supplemental instrument to existing grant) Role: PI Sponsor: National Science Foundation (CHE – Experimental Physical Chemistry) Title: Protein dynamics and hydrogen tunneling in enzymatic catalysis (PI: Kohen) Supplemental Instrumentation

Amount: $40,000 Duration: Aug. 2014 BSF Role: PI Sponsor: United States – Israel Binational Foundation Title: Protein dynamics in Enzymology (PIs: Kohen & Major)

Amount: $234,000 Duration: Oct. 2013 – Sept. 2017

NIH-RO1 Role: Co-PI (50%) (PI: Cheatum) Sponsor: National Institutes of Health (NIGMS) Title: The role of fs-ps dynamics in enzymatic H-transfer

Amount: $1,444,000 Duration: June 2010 – May 2015 ICRU Role: PI Iowa Center for Research by Undergraduate Students Title: Use of Heavy Enzyme to Explore Chemical Steps Catalyzed by Thymidylate Synthase (PI: Kohen; Advisee: Catherine Suchanek)

Amount: $ 2,000 Duration: Aug 2014 – May 2015

PENDING GRANTS:

Beckman Scholars at Iowa Role: Co-Investigator Sponsor: Beckman Title: Undergraduate Research at the Interfaces of Chemistry and Biomedicine (PI: Brenner)

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TRAINING GRANTS

NIH – T32 Role: PI (Director and Trainer) Sponsor: National Institutes of Health (NIGMS) Title: Predoctoral Training Program in Biotechnology

Amount: $1,019,700 Duration: July 2011 – June 2016 MSTP Training Grant Role: Trainer (Mike Knudson PI) Sponsor: National Institutes of Health MCB Training Grant Role: Trainer (Jackie Bickenbach PI) Sponsor: National Institutes of Health

PAST GRANTS:

NIH-RO1 (Renewal of the original 2002 award) Role: PI Sponsor: National Institutes of Health (NIGMS – RO1) Title: Tunneling and dynamics in enzyme catalyzed reactions (PI: Kohen)

Amount: $1,108,352 Duration: July 2009 – March 2014

Biological Science Funding Program (BSFP) Role: PI Sponsor: UI, OVPR Title: New Radiotracer for Cancer Imaging (PI: Kohen)

Amount: $25,000 Duration: June 2011 – May 2012

Clinical and Translational Science Award Role: PI Sponsor: Institute for Clinical Translational Science (ICTS) Title: Astrogene effects on cancerues cells

Amount: $50,000 Duration: June 2011 – May 2012

Chemistry Shared instrumentation facility Role: Contributor Sponsor: Roy J. Carver Charitable Trust Title: Acquisition of an Isothermal Titration Calorimetry and a Size Exclusion Separation Apparatus. Amount: $230,689 Awarded: January 2012

NSF (Supplemental instrument to existing grant) Role: PI Sponsor: National Science Foundation (CHE – Experimental Physical Chemistry) Title: Protein dynamics and hydrogen tunneling in enzymatic catalysis (PI: Kohen) Supplemental Instrumentation

Amount: $66,000 Duration: Aug. 2010

NSF (Renewal of the 2002 CAREER AWARD) Role: PI Sponsor: National Science Foundation (CHE – Experimental Physical Chemistry) Title: Protein dynamics and hydrogen tunneling in enzymatic catalysis (PI: Kohen)

Amount: $500,000 Duration: Aug 2007 – July 2011 plus one year “no-cost extension”

BSF Role: PI Sponsor: United States – Israel Binational Foundation Title: Protein dynamics in Enzymology (MPIs: Kohen & Major)

Amount: $204,000 Duration: Oct. 2008 – Sept. 2012

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Clinical and Translational Science Award Role: PI Sponsor: Institute for Clinical Translational Science (ICTS) Title: New PET imaging of cancerous tumors

Amount: $50,000 Duration: June 2009 – May 2010 Faculty Scholars Award (supplemental) Role: PI Sponsor: University of Iowa In addition to half my salary, each year I received $5,500 as supplemental funding from the UI Office of the Provost. Duration: 2007 – May 2010

ICRU Scholar Assistant program Role: PI Sponsor: University of Iowa Title: Mechanistic Studies of TSase from E. coli (PI: Kohen; Advisee: Michael Toraason)

Amount: $2,500 Duration: June. 2010 – Aug. 2010 ICRU Scholar Assistant program Role: PI Sponsor: University of Iowa Title: Isotopic labeling of nicotinamides (PI: Kohen; Advisee: Hyun Jo)

Amount: $2,500 Duration: Oct. 2008 – May 2009 NIH-RO1 Role: PI Sponsor: National Institutes of Health (NIGMS) Title: Tunneling and dynamics studies with DHFR (PI: Kohen)

Amount: $884,400 Duration: July 2002 – June 2008 NSF CAREER Role: PI Sponsor: National Science Foundation (CHE – Experimental Physical Chemistry) Title: CAREER: Protein dynamics and hydrogen tunneling in enzymatic catalysis (PI: Kohen)

Amount: $440,500 Duration: June 2002 – May 2007 Herman Frasch Grant Role: PI Sponsor: Herman Frasch Foundation Title: Studies of nitrogen fixation by the enzyme nitrogenase (PI: Kohen)

Amount: $200,000 Duration: July 2002 – June 2007

IREU – Iowa Research Experience for Undergraduates Role: PI Sponsor: University of Iowa Title: Flavin-dependent thymidylate synthase (PI: Kohen)

Amount: $3,000 Duration: Dec. 2006 – Aug. 2007 (to sponsor Undergraduate research).

IREU – Iowa Research Experience for Undergraduates Role: PI Sponsor: University of Iowa Title: 2 KIE studies of nicotinamide dependent enzymes (PI: Kohen)

Amount: $3,000 Duration: Dec. 2004 – Aug. 2005 (to sponsor Undergraduate research).

NIH – R21 Role: PI Sponsor: National Institutes of Health Title: Mechanistic studies of nitrogenase catalysis (PI: Kohen)

Amount: $220,500 Duration: July 2002 – June 2004

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ACS – PRF, Type G Role: PI Sponsor: American Chemical Society, Petroleum Research Fund Title: C-H bond activation in enzyme catalyzed reaction (PI: Kohen)

Amount: $25,000 Duration: Sep. 2001 – Sep. 2003 ACS – PRF, Type SE Role: PI Sponsor: American Chemical Society, Petroleum Research Fund Title: Symposium on structure-function correlation in enzyme action (PI: Kohen)

Amount: $3,600 Duration: Apr. 2002 Biosciences Initiative – Pilot Grant Program Role: PI Sponsor: University of Iowa Title: The role of protein dynamics coupled motion and tunneling in enzyme catalysis

(PI: Kohen) Amount: $50,000 Duration: Nov. 2000 – June 2001

CIFRE (Central Investment Fund for Research Enhancement) Role: PI Sponsor: University of Iowa Title: Biological catalysis – mechanistic studies (PI: Kohen)

Amount: $9,977 Duration: Jan. 2000 – June 2000

Invited lectures External invited lectures: Conferences

1. “Standards in isotopic measurements”, 7th Beilstein ESCEC Symposium, Ruedesheim, Germany, Sept. 14- 18, 2015.

2. “Functional enzyme dynamics at various timescales”, Telluride Workshop, Telluride CO, Aug. 3-7, 2015.

3. “Adiabatic vs. Non-Adiabatic H-transfers in Enzyme Catalyzed Reactions”, an Invited Talk at the ACS Phys. Chem. Symposium on Computational Chemical Dynamics in Honor of Don Truhlar, 249th ACS National Meeting, Denver, CO, March 22-26, 2015.

4. “Enzyme Mechanisms: From Physical Chemistry to Evolution and New Drug Targets”, an Invited Talk at the ACS Graduate Student Symposium, 249th ACS National Meeting, Denver, CO, March 22-26, 2015.

5. “Enzyme dynamics in thymidylate synthase sequential reactions”, International Enzyme Mechanism Conference (EMBO), Manchester, UK, June 1-4, 2014.

6. “Mechanistic studies of different thymidylate synthases and the role of protein dynamics in catalysis”, ASBMB Annual Meeting, San Diego, CA, April 26-30, 2014.

7. “Role of dynamics and H-tunneling in enzyme catalysis”, Telluride Workshop, Telluride CO, July 29 - Aug. 2, 2013.

8. “Flavin dependent thymidylate synthase”, Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley Resort in Waterville, NH, July 14-19, 2013.

9. “Temperature dependence of kinetic Isotope effects in enzymology”, ISOTOPES 2013, Sopot, Poland, June 16-21, 2013.

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10. “Protein dynamics in enzyme catalyzed reactions” 96th Canadian Chemistry Conference and Exhibition, Québec City, Canada, May 26 – 30, 2013.

11. “Role of dynamics in enzyme catalyzed reactions”, 245th ACS National Meeting, New Orleans, LA, April 7-11, 2013.

12. “Alternative mechanism for thymine biosynthesis”, 245th ACS National Meeting, New Orleans, LA, April 7-11, 2013.

13. “Structural - mechanistic studies of flavin-dependent thymidylate synthase”, 23rd Enzyme Mechanisms Conference, Loews Coronado Bay, Coronado CA, FL, Jan. 3-7, 2013.

14. “The role of dynamics and H-tunneling in enzyme catalysis”, Zing conference on Enzymes, Coenzymes, and Metabolic Pathways, Occidental Grand Xcaret resort, Mexico, November 17-21, 2011.

15. “Intrinsic kinetic isotope effects in enzymology”, ISOTOPE 2011, Gréoux-les-Bains, France, June 20-24, 2011.

16. “Thymidylate synthase: Quantitative and qualitative mechanistic studies”, Klinman Symposium, Berkeley, CA, April 16-17, 2011.

17. “Paths of de novo thymidylate biosynthesis“, 19th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids, Lyon, France, Aug. 29 - Sept. 3, 2010.

18. “Enzymatic H-Transfer: From Organic Mechanisms to Quantum Effects”, Theoretical Chemistry Symposium. Lodz, Poland, March 30-31, 2010.

19. “Qualitative and quantitative use of isotopes in mechanistic studies of enzymes”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences. Hotel Galvez in Galveston, Texas, Feb. 14-19, 2010.

20. “Enzyme catalyzed H-transfer: The role of coupled environmental dynamics”, 2010 Gentner Symposium on “Proton Mobility in Chemical and Biological Systems”, Ma`agan Holiday Village, Israel, Feb. 7-12, 2010.

21. “Alternative thymidylate synthase”, The Annual Meeting of the Israeli Society of Biochemistry and Molecular Biology (ISBMB), Rechovot, Israel, Feb. 4, 2010.

22. “The role of enzyme dynamics in C-H bond activation“, Telluride Science Research Center Workshop on Protein Dynamics, Telluride, Colorado, Aug. 3 - 7, 2009.

23. “An unusual mechanism of thymidylate biosynthesis in organisms containing the thyX gene”, Gordon Research Conference on Nucleosides, Nucleotides, and Oligonucleotides, Salve Regina University, Newport, RI, July 5-10, 2009. [I could not make it to this meeting and my student, Eric Koehn, presented on our behalf]

24. “Isotopic labeling and kinetic isotope effects in Enzymology”, Isotope 2009, Cluj - Napoca, Romania, May 25 - 29, 2009.

25. “Protein Dynamics and Enzyme Catalysis – Environmentally Coupled Hydrogen Tunneling”, A Mesilla Chemistry Workshop “Multi-Scale Modeling of Biological Molecules” Meson de Mesilla, NM, Feb. 4-7, 2009.

26. “Environmental dynamics in enzyme catalyzed reactions”, Second International Interdisciplinary Conference on Vitamins, Coenzymes and Biofactors (2nd IICVCB), Athens, GA, Oct. 26-31 2008.

27. “KIEs and their temperature dependency as probes for dynamics and coupling”, Conference on Protein Dynamics and Catalysis Tarrytown, NY, May 2-4, 2008.

28. “H-tunneling in enzymatic systems”, Israel Science Foundation Workshop on Diffusion, Solvation, and Transport of Protons in Complex and Biological Systems, Eilat, Israel, Jan. 13-17, 2008.

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29. "Enzymatic redox reactions: Hydride and proton transfer mechanisms using kinetic isotope effects", Stable Isotope Tools for the Assessment of Chemical and Microbial Transformation Reactions in Complex Natural and Contaminated Environments, Mt. Verità, Switzerland, Nov. 18- 23, 2007.

30. "The Temperature Dependence of Isotope Effects in Biological Catalysis", Quantum Atomic and Molecular Tunneling in Solids & other Condensed Phases, Huston, TX, Oct. 28-Nov.1, 2007.

31. "Intrinsic Kinetic Isotope Effects As Mechanistic Probes in Enzymology", Isotope 2007, Benicassim, Spain, May 27-June 1, 2007.

32. "A Network of Coupled Motions and H-Tunneling in Enzyme Catalysis", 20th Enzyme Mechanisms Conference St. Pete Beach, FL, Jan. 3-6th, 2007.

33. “H-transfer in Enzymology”, Chem06 University of Cairo, Egypt, 5-8th Mar. 2006.

34. “Effects of Distal Mutations on Enzymatic Activity as Probed by Vibrationally Coupled Tunneling”, Royal Society Meeting on “Quantum Catalysis in Enzymes- Beyond the Transition State Theory Paradigm”, London UK, Nov. 14-16 2005.

35. “Tunneling and coupled motion in enzymatic catalysis”, International Conference on Quantum Atomic and Molecular Tunneling in Solids (QAMTS), Santiago de Compostela, Spain, July 19-21, 2005.

36. “Isotope effects as probes for tunneling and coupled motion in enzyme catalysis”, Isotopes 2005, Bath UK, June 27-30 2005.

37. “Protein dynamics role in enzyme catalysis”, Conference by the Mathematical Biosciences Institute on “Enzyme Dynamics”, Columbus, OH, May 19-21, 2005.

38. “Nitrogenase catalyzed N2 reduction: 15N isotope effects studies”, Gordon Research Conference on Nitrogen Fixation, Colby Sawyer College, NH, July 20-25, 2004.

39. “Coupled motion and H-tunneling in the dihydrofolate reductase reaction”, 56th Harden Conference - Biological Electron and Proton Transfer. University of Plymouth, UK, Aug. 26-30 2003.

40. “H-Tunneling and Coupled Motion in Enzyme Catalysis”. International Conference on Quantum Atomic and Molecular Tunneling in Solids (QAMTS), Gainesville, FL, June 22-25, 2003.

41. “Kinetic Isotope Effects in Enzymology”, 54th Harden Conference on 'Enzymology: emerging trends and future prospects', Ambleside (Cumbria), UK. Aug. 20–24, 2002.

42. “Enzyme Catalysis: the Role of H-Tunneling and Protein Dynamics”, 10th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 22-24, 2001.

43. “Competitive Kinetic Isotope Effects with 2’-Phosphate Nicotine-Adenine-Dinucleotide”, 2001 – An Isotope Odyssey, Zakopane, Poland, June 24-29, 2001.

44. “Enzyme Dynamics and Catalysis Relationship As Probed by H-Tunneling and Coupled Motion”, 20th Midwest Enzyme Chemistry Conference, Chicago, IL, Sep. 23, 2000.

45. “Protein Dynamics and Enzyme Catalysis - Vibrationally enhanced Hydrogen Tunneling”, Third Mesilla Chemistry Workshop “Dynamics of Enzyme-Catalyzed Reactions” Meson de Mesilla, NM, Feb. 6-9, 2000.

46. “Enzyme Dynamics and Hydrogen Tunneling in a Thermophilic Alcohol Dehydrogenase”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences. Holiday Inn, Ventura, CA, Jan. 9-14, 2000.

Contributed Talks (Chosen by organizers based on submitted abstract)

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1. “The role of enzyme dynamics in catalysis”, Bar-Ilan University, Tel-Aviv, Israel, Dec. 29, 2013.

2. “Mechanistic studies of thymidylate Synthases”, Texas Enzyme Mechanisms Conference, Austin Jan. 8, 2012.

3. “Structure-dynamics-function relationship. A new perspective”, 237th ACS National Meeting, Salt Lake City, Utah, Mar. 22-26, 2009.

4. “H-tunneling in enzymatic systems”, Symposium on Computational Chemical Dynamic: From Gas-Phase to Condensed-Phase Systems, University of Minnesota, Minneapolis, MN, Oct. 7-9, 2004.

5. “Environmentally Coupled Tunneling in Enzymatic Hydride Transfer Reactions”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences. Holiday Inn, Ventura, CA, Feb. 15-20, 2004.

6. “Studying the chemical step in enzyme catalysis”, 223rd ACS National Meeting, Orlando, FL, Apr. 7-11, 2002.

External invited lectures: Universities 1. “H-tunneling and the role of protein dynamics in enzyme catalysis ", Departmetn of Chemistry, Uppsala

University, Uppsala, Sweden, May 28, 2014.

2. “The role of enzyme dynamics in catalysis”, Department of Chemistry, Bar-Ilan University, Israel, Dec. 29, 2013.

3. “Interactive theoretical-experimental studies of enzyme activated C-H bonds”, Dept. Química Física i Analítica, Universitat Jaume I, Castello, Spain, May 17, 2012.

4. “Physical and Organic Mechanisms in Enzymology”, Umeå, Sweden, March 6, 2012.

5. “Quantum and Dynamics Effects in biological Redox Reactions Involving C-H Bond Activation”, The Technion-Israel Institute of Technology, Haifa, Israel, Feb 28, 2012.

6. “Physical and Organic Mechanisms in Enzymology: Thymine Biosybthesis”, Department of Chemistry, University of Kansas, Lawrence, KN, Nov 11, 2011.

7. “Physical and Organic Mechanisms in Enzymology”, Department of Chemistry, Wichita State University, Wichita, MI, Nov 2, 2011.

8. “Enzyme Mechanisms: Physical and Organic Chemistry Perspective”, Department of Medicinal & Bioorganic Chemistry, University of Maryland at Baltimore Co, Oct 26, 2011.

9. “Enzymology: from physical and organic mechanisms to biochemical pathways”, Department of Chemistry and Biochemistry, University of Texas, Arlington, TX, Oct 21, 2011.

10. “Resolving the alcohol dehydrogenase paradox”, Department of Chemistry, Lodz University, Poland, March 23, 2010.

11. “Enzyme dynamics and H-tunneling in the dihydrofolate reductase reaction”, Department of Biochemistry, Tel-Aviv University, Israel, Feb. 2, 2010.

12. “Thymidylate synthases: From organic mechanisms to physical studies”, Department of Biochemistry, Pennsylvania State University, Oct 6, 2009.

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13. “Mechanisms of C-H bond Activation in enzyme catalysis: From arrow-pushing to quantum mechanics”, Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, NY, July 22, 2009.

14. “Dihydrofolate reductase as a model for enzyme structure-dynamics-function relationship”, Division of Structural Biology, The Weizmann Institute of Science, Rechovot, Israel, June 30, 2009.

15. “Mechanisms of C-H bond activation in enzyme catalysis”, Colloquium at the School of Chemistry, The Tel Aviv University, Tel Aviv, Israel, June 9, 2009.

16. “Physical description of enzyme catalyzed H-transfer”, Colloquium at the Department of Chemistry, The Weizmann Institute of Science, Rechovot, Israel, June 8, 2009.

17. “Quantum tunneling in Enzymology”, Manchester Interdisciplinary Biocentre, University of Manchester, Manchester, UK, May 14, 2009.

18. “Mechanistic Studies of Thymidylate Synthases: From QM-Tunneling to Organic Mechanisms”, Colloquium at the Department of Chemistry, Technion-Israel Institute of Technology, Haifa, Israel, May 7, 2009.

19. “Enzyme Catalyzed C-H Bond Activation: Role of H-tunneling and Coupled Dynamics”, Department of Chemistry, University of Michigan, Nov. 21, 2008.

20. “Role of the Protein Dynamics and H-Tunneling in Enzyme Catalysis”, Department of Chemistry, Bar Ilan University, Israel, Jan. 23, 2008.

21. “Enzyme Mechanisms from Isotope Effects”, Department of chemistry, University of California, Davis, CA, Nov. 8, 2007.

22. "Mechanistic studies of flavin-dependent thymidylate synthase", College of Pharmacy, Texas University, Austin, TX, Nov. 17, 2006.

23. "Distal effects in enzyme catalysis" Department of Biochemistry and Department of Chemistry, Texas A&M University, Collage Station, TX, Nov. 16, 2006.

24. “Tunneling and vibrational coupling in biological systems”, Department of Chemistry, University of Vienna, Vienna, Austria, Oct. 6, 2006.

25. “The role of protein motion and H-tunneling in thymine biosynthesis”, Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, Aug. 25, 2006.

26. “Enzyme dynamics and H-tunneling in Biocatalysis”, Department of Biochemistry and Cellular & Molecular Biology University of Tennessee, Knoxville, TN, Apr. 26, 2006.

27. “Hydrogen transfer in enzyme catalysis: Studies of dihydrofolate reductase and thymidylate synthase”, Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX, Apr. 7, 2006.

28. “H-tunneling and Matrix dynamics in Enzymology”, Departmental Seminar at the Department of Chemistry, University of Southern California, Los Angeles, CA, Feb 28, 2006.

29. “Effects of remote mutations on H-transfer in DHFR catalysis”, Department of Chemistry, Cardiff University, UK, Nov. 15, 2005.

30. “Studies of an alternative thymidylate synthase”, Laboratoire d'Optique et Biosciences, Ecole Polytechnique, Palaiseau Cedex, France, Nov. 9, 2005.

31. “Classical and flavin-dependent thymidylate synthases”, departmental seminar at the Department of Chemistry, Universitat Jaume I, Castellon, Spain, July 26 2005.

32. “Studying the chemical step in nitrogenase-catalyzed N2 reduction”, departmental seminar at the Department of Chemistry and Biochemistry, UC Irvine, CA, Apr. 15, 2005.

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33. “Mechanistic studies of DHFR and its distal mutants”, departmental seminar at the Department of Chemistry, John Hopkins University, MD, Mar. 8, 2005.

34. “Mechanistic studies of DHFR and its distal mutants”, departmental seminar at the Department of Chemistry, University of Delaware, DE, Mar. 7, 2005.

35. “Protein Dynamics Effects on DHFR Catalysis”, Department of Chemistry Colloquium, Utah State University, UT, Mar. 3, 2005.

36. “Bio-Physical-Organic Chemistry Studies of Enzyme Catalysis”, departmental seminar at the Department of Chemistry, University of Northern Iowa, IA, Nov. 11, 2004.

37. “Mechanistic Studies of Flavin-Dependent Thymidylate Synthase”, Rosalind Franklin University of Medicine and Science, Chicago, IL, Nov. 4, 2004.

38. “Tunneling, Coupled Motion, and Dynamics in Enzymatic Reactions”, Department of Chemistry Colloquium, Universitat Autonoma de Barcelona, Barcelona, Spain, Sept. 4, 2003.

39. “Studying the Chemical Step in Enzyme Catalyzed Reactions”, interdepartmental seminar at Universitat Pompeu Fabra, Barcelona, Spain, Sept. 3, 2003.

40. “Enzyme Dynamics and Hydride Transfer in Biocatalysis”, Biochemistry Colloquium. University of Nebraska, Lincoln, NE. Mar. 4, 2003.

41. “Chemical Catalysis In Biology: How Enzymes Activate C-H bonds”, ACS Chapter of Northeast Arkansas meeting. Lyon College, AR. Feb. 25, 2003.

42. “H-tunneling in Enzymology”, Physical Chemistry Seminar at the University of Notre Dame, IN, Nov. 8, 2001.

43. “Enzyme Catalysis: the Role of Protein Dynamics, H-Tunneling and Coupled Motion”, departmental seminar at the Department of Physiology & Biophysics, Albert Einstein College of Medicine, NY, Apr. 12, 2001.

44. “Kinetic Isotope Effect Studies of H-Tunneling in Enzyme Catalyzed Reactions”, Organic Division seminar at the Department of Chemistry, Technion – Israel Institute of Technology, Israel, Nov. 23, 2000.

45. “Protein Dynamics and Enzyme Catalysis”, Departmental Seminar at the Department of Biochemistry, Tel-Aviv University, Israel, Nov. 16, 2000.

University of Iowa Invited Seminars 1. “The chemistry of enzyme evolution”, MCB seminar, Nov. 6, 2014.

2. “Development of a new PET radiotracer for cancer, targeting de novo thymine biosynthesis", Translational Research Meeting, Department of Radiation Oncology, May 25, 2012.

3. “Biophysical and Mechanistic Studies of Thymidylate Synthase”, Department of Molecular Physiology & Biophysics (Host: Kevin Campbell), March 21, 2012.

4. "Development of new radio-tracer for cancer imaging", The Iowa Institute for Biomedical Imaging (IIBI) - Weekly Medical Imaging Seminar, March 24, 2011.

5. “Mechanistic Studies of Enzyme Catalyzed C-H Bond Activation”, Department of Chemistry Colloquium, University of Iowa, IA, Oct. 6, 2009.

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6. “Enzyme catalysis: From organic mechanisms to physical studies”, Department of Biochemistry, University of Iowa, IA, Sept. 17, 2009.

7. “Mechanistic Studies of Enzyme Catalyzed C-H Bond Activation”, Department of Chemistry colloquium, University of Iowa, IA, Oct. 15, 2004.

8. “Thymidylate Synthase and Dihydrofolate Reductase: Mechanistic Studies of Enzyme-Catalyzed Hydride Transfer”, Department of Biochemistry, University of Iowa, Jan. 29, 2004.

9. “Hydrogen Tunneling in Enzyme Catalysis”, Physical Chemistry seminar at the University of Iowa, Oct. 21, 2002.

10. “Kinetic Isotope Effects in Studying Hydrogen Tunneling”, Medicinal and Natural Products Chemistry seminar, College of Pharmacy, University of Iowa, Mar. 20, 2000.

Poster and Oral Presentations in Conferences by Students (Underlined names are presenting authors, and Oral Presentations are specified) Poster and oral presentations of work performed at Iowa:

1. Mishanina, T.V., Kohen, A. “Unusual flavin derivative intermediate in FAD-dependent thymidylate synthase”, 34th Midwest Enzyme Chemistry Conference”, Northwestern University, Chicago, IL, Sept. 27, 2014. (Oral Presentation)

2. Abeysinghe, T., Suchanek, C., Kohen, A., “Experimental Studies of Enzyme Catalyzed Hydrogen-Transfer Reactions”, 34th Midwest Enzyme Chemistry Conference”, Northwestern University, Chicago, IL, Sept. 27, 2014.

3. Francis, K., Kohen, A., “Preservation of Protein Dynamics in Enzyme Evolution”, 34th Midwest Enzyme Chemistry Conference”, Northwestern University, Chicago, IL, Sept. 27, 2014.

4. Ghosh, A., Krueger, K., Kohen, A., “Investigating the Role of Tyrosine 94 in Thymidylate Synthase”, 34th Midwest Enzyme Chemistry Conference”, Northwestern University, Chicago, IL, Sept. 27, 2014.

5. Guo, Q., Cheatum, C.M., Kohen, A., “Structural and Kinetic Studies on Formate Dehydrogenase from Candida boidinii”, 34th Midwest Enzyme Chemistry Conference”, Northwestern University, Chicago, IL, Sept. 27, 2014.

6. Gurevic, I., Karunaratne, K., Mondal, D., Kohen, A., “Alternative Quencher and Substrate Candidates in Mechanistic Studies of Flavin-Dependent Thymidylate Synthase”, 34th Midwest Enzyme Chemistry Conference”, Northwestern University, Chicago, IL, Sept. 27, 2014.

7. Islam, Z., Strutzenberg, T.S., Gurevic, I., Kohen, A., “Concerted versus Step-wise Mechanism in Thymidylate Synthase”, 34th Midwest Enzyme Chemistry Conference”, Northwestern University, Chicago, IL, Sept. 27, 2014.

8. Pagano, P., Guo, Q., Kohen, A., Cheatum, C.M., “Investigating Enzyme Dynamics at Fast Timescales Using Two Dimensional Vibrational Spectroscopy”, 34th Midwest Enzyme Chemistry Conference”, Northwestern University, Chicago, IL, Sept. 27, 2014.

9. Ranasinghe, C., Guo, Q., Kohen, A., “Protein Mass-modulated Effects on Function Relevant Dynamics of Formate Dehydrongenase”, 34th Midwest Enzyme Chemistry Conference”, Northwestern University, Chicago, IL, Sept. 27, 2014.

Kohen, A.

22

10. Singh, P., Kohen, A., “Linking Protein Motions to Enzyme Catalysis: Explorations in Enzymology”, 34th Midwest Enzyme Chemistry Conference”, Northwestern University, Chicago, IL, Sept. 27, 2014.

11. Ye, H., Guo, Q., Kohen, A., “Characterization and Kinetic Studies on Mutants of Formate Dehydrogenase from Candida boidinii”, 34th Midwest Enzyme Chemistry Conference”, Northwestern University, Chicago, IL, Sept. 27, 2014.

12. Abeysinghe, D.T., Kohen, A., “Mechanistic studies of thymidylate synthase”, HCCC retreat, Coralville, IA, June 16, 2014.

13. Singh, P.N., Kohen, A., “Global and local effects on enzyme dynamics: Studies of dihydrofolate reductase”, HCCC retreat, Coralville, IA, June 16, 2014

14. Francis, K., Kohen, A., “Exploring the Evolution of Protein Dynamics in Dihydrofolate Reductase through the Temperature Dependence of Intrinsic Kinetic Isotope Effects”, 3rd Southeast Enzyme Mechanism Conference, Atlanta, GA, April 5, 2014. (Oral Presentation)

15. Abeysinghe, T., Suchanek, C., Kohen, A., “Initial Velocity Studies Comparing Heavy and Light Thymidylate Synthase”, College of Engineering research open house, Iowa City, April 10, 2014.

16. Francis, K., Kohen, A., “Preservation of protein dynamics in dihydrofolate reductase evolution”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences, Galveston, Texas, Feb. 2-70, 2014. (Oral Presentation)

17. Abeysinghe, T., Kohen, A., “Kinetic isotope effects study of thymidylate synthase”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences, Galveston, Texas, Feb. 2-70, 2014.

18. Mishanina, T.V., Kohen, A., “An unusual flavin-nucleotide covalent adduct in the biosynthesis of thymine”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences, Galveston, Texas, Feb. 2-70, 2014.

19. Singh, P., Kohen, A., “Global and local effects on enzyme dynamics: Studies of dihydrofolate reductase”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences, Galveston, Texas, Feb. 2-70, 2014.

20. Strutzenberg, T., Islam, Z., Kohen, A., "Kinetic Isotope Effects as probes for the Hydride Transfer in E. Coli Thymidylate Synthase." ACS Iowa/Illinois Undergraduate Research Conference, St. Ambrose University, Davenport, IA, Nov. 16, 2013

21. Islam, Z., Kohen, A., “Kinetic Isotope Effects to Probe the Hydride-Transfer Mechanism in E.coli Thymidylate Synthase”, 22nd Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 21-22, 2013.

22. Singh, P. N., Kohen, A., “Dihydrofolate reductase role in catalysis”, 22nd Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 21-22, 2013.

23. Ghosh, A.K., Kohen, A., “Effects of Y94F in the proton abstraction step in thymidylate synthase”, 22nd Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 21-22, 2013.

24. Karunaratne, K.U., Mishanina, T.V., Foss Jr. F.W., Kohen, A., “Exploring the mechanism of FDTS Flavin-Dependent Thymidylate Synthase”, 22nd Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 21-22, 2013.

25. Abeysinghe, T., Kohen, A., “Effects of a remote mutation on different chemical steps catalyzed by Thymidylate Synthase”, 22nd Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 21-22, 2013.

Kohen, A.

23

26. Mishanina, T.V., Corcoran, J.M., Koehn, E.M., Lesley, S.A., Kohen, A., "Mechanism of Oxidative Half-Reaction of Flavin-Dependent Thymidylate Synthase", 22nd Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 21-22, 2013.

27. Guo, Q., Gakhar, L., Wickersham, K., Francis, K., Cheatum, C.M., Kohen, A., “Structural and Kinetic Studies of Formate Dehydrogenase”, 22nd Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 21-22, 2013.

28. Abeysinghe, T., Kohen, A., “Effects of a remote mutation on different chemical steps catalyzed by Thymidylate Synthase”, 22nd Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 21-22, 2013.

29. Suchanek, C., Abeysinghe, D.T., Kohen, A., “Method of Using Fastcloning Technique to Express Thymidylate Synthase with Polyhistidine-Tag to Aide in the Extraction of a Pure Protein”, AICHE, 2013. XX

30. Pagano, P., Cheatum, C.M., Kohen, A., “Incorporation of Thiocyanate as an Infrared Probe for Fast Dynamics at the Active-site of E. coli Dihydrofolate Reductase”, XXXIII Midwest Enzyme Chemistry Conference”, Layola University, Chicago, IL, Oct. 12, 2013.

31. Abeysinghe, D.T., Kohen, A., “The role of protein’s long-range dynamics in thymidylate synthase catalyzed reactions”, XXXIII Midwest Enzyme Chemistry Conference”, Layola University, Chicago, IL, Oct. 12, 2013.

32. Ghosh, A., Kohen, A., “The role of protein’s long-range dynamics in thymidylate synthase catalyzed reactions”, XXXIII Midwest Enzyme Chemistry Conference”, Layola University, Chicago, IL, Oct. 12, 2013.

33. Islam, Z., Kohen, A., “Concerted vs. Stepwise Hydride-Transfer in E. Coli Thymidylate Synthase”, XXXIII Midwest Enzyme Chemistry Conference”, Layola University, Chicago, IL, Oct. 12, 2013.

34. Karunaratne, K.U., Mishanina, T.V., Foss, F.W., Kohen, A., “5-deaza-FAD as a mechanistic probe to study FDTS Flavin-dependent thymidylate synthase”, XXXIII Midwest Enzyme Chemistry Conference”, Layola University, Chicago, IL, Oct. 12, 2013.

35. Mishanina, T.V., Koehn, E.M., Conrad, J.A., Palfey, B., Lesley, S.A., Kohen, A., “Elucidation of the chemical mechanism of alternative thymidylate synthase”, XXXIII Midwest Enzyme Chemistry Conference”, Layola University, Chicago, IL, Oct. 12, 2013.

36. Morgan, S., Koehn, E.M., Kohen, A., “Elucidation of the chemical mechanism of alternative thymidylate synthase”, XXXIII Midwest Enzyme Chemistry Conference”, Layola University, Chicago, IL, Oct. 12, 2013.

37. Roston, D., Lu, Y., Kohen, A., “Comparing Alcohol Oxidation by Alcohol Dehydrogenase with an Analogous Uncatalyzed Reaction”, XXXIII Midwest Enzyme Chemistry Conference”, Layola University, Chicago, IL, Oct. 12, 2013.

38. Singh, P., Wang, Z., Schramm, V.L., Kohen, A., “Enzyme dynamics role in catalysis: Study of Dihydrofolate Reductase”, XXXIII Midwest Enzyme Chemistry Conference”, Layola University, Chicago, IL, Oct. 12, 2013.

39. Wickersham, K., Gao, Q., Cheatum, C.M., Kohen, A., “Characterization of Catalytic Properties of Formate Dehydrogenase”, XXXIII Midwest Enzyme Chemistry Conference”, Layola University, Chicago, IL, Oct. 12, 2013.

40. Abeysinghe, T., Suchanek, C., Kohen, A., “Use of Fastcloning technique to clone a gene into a vector with a polyhistidine tag”, Summer undergraduate Research Conference, The University of Iowa, IA, July 31

, 2013.

Kohen, A.

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41. Wang, Z., Singh, P. N., Czekster, C., Kohen, A., Schramm, V. P., “Mass-modulated Catalytic Properties of Dihydrofolate Reductase”, Gordon Research Conferences on Enzymes, Coenzymes & Metabolic Pathways, Waterville Valley Resort, Waterville Valley, NH, July 18, 2013. (won the Jeremy Knowles Travel Award for best poster)

42. Singh, P. N., Kohen, A., “Extending the network of coupled dynamics across the Protein: Studies of Dihydrofolate Reductase”, Holden Comprehensive Cancer Center Research Retreat, The University of Iowa, Iowa City, IA, June 13, 2013.

43. Abeysinghe, T., Wang, Z., Kohen, A., “Histidine 147 and its implications on the mechanism of Thymidylate Synthase”, Holden Comprehensive Cancer Center Research Retreat, The University of Iowa, Iowa City, IA, June 13, 2013.

44. Mishanina, T.V., Lesley, S.A., Kohen, A., "Exploring the Chemical Mechanism of Flavin-Dependent Thymidylate Biosynthesis," NIH/CBB Training Program Symposium, Iowa City, IA, May 16, 2013. (Oral Presentation)

45. Singh, P. N., Kohen, A., “Extending the network of coupled dynamics across the Protein: Studies of Dihydrofolate Reductase”, The 15th Annual James F. Jakobsen Graduate Conference, The University of Iowa, Iowa City, IA, April 5-6 , 2013.

46. Abeysinghe, T., Kohen, A., “Active site muataion and altered chemical steps in the mechanism of Thymidylate Synthase”, The 15th Annual James F. Jakobsen Graduate Conference, The University of Iowa, Iowa City, IA, April 5-6 , 2013. (Oral Presentation)

47. Guo, Q., Cheatum, C.M., Kohen, A., “Remote Mutants Affect Enzyme Active Site Dynamics Revealed by Two Dimensional Infrared Spectroscopy”, 15th Annual James F. Jakobsen Conference, the University of Iowa, Iowa City, IA, Apr. 6, 2013. (Oral Presentation)

48. Mishanina, T.V., Koehn, E.M., Conrad, J.A., Palfey, B.A., Lesley, S.A., Kohen, A., "Mechanistic Studies of a New Antimicrobial Enzyme Target," 15th Jakobsen Graduate Conference, Iowa City, IA, April 5-6, 2013. (Oral Presentation)

49. Pagano, P., Cheatum, C.M., Kohen, A., "The Dynamic Nature of Dihydrofolate Reductase", Optical Science Technology Center, Iowa City, IA, March 8, 2013.

50. Guo, Q., Cheatum, C.M., Kohen, A., “Applications of Two-Dimensional Infrared Spectroscopy: Dynamics in Enzymes and solutions”, Optical Science and Technology Center 11th Annual Symposium, the University of Iowa, Iowa City, IA, Mar. 8, 2013.

51. Wang, Z., Kohen, A., “Experiments and calculations indicating new thymidylate synthase intermediate”, 23rd Enzyme Mechanisms Conference, Loews Coronado Bay, Coronado CA, FL, Jan. 3-7, 2013 (Oral presentation as the conference’s Founder's Award Lecture to Zhen Wang, plus $2,000 to cover travel expenses).

52. Mishanina, T., Kohen, A., “Unraveling the mechanism of a novel thymidylate biosynthesis enzyme”, 47th ACS Midwest Regional Meeting, Omaha, NE, Oct. 24-27, 2012 (Oral presentation).

53. Guo, Q., Kohen, A., “Remote Mutant Studies Reveal Altered Active Site Dynamics of Formate Dehydrogenase”, 47th ACS Midwest Regional Meeting, Omaha, NE, Oct. 24-27, 2012 (Oral presentation).

54. Singh, P.N., Kohen, A., “Network of Coupled Dynamics across the Protein: Studies of Dihydrofolate Reductase”, 47th ACS Midwest Regional Meeting, Omaha, NE, Oct. 24-27, 2012 (Oral presentation).

55. Abeysinghe, D.T., Kohen, A., “Effects of Y209W on the Hydride transfer step in Thymidylate synthase”, 47th ACS Midwest Regional Meeting, Omaha, NE, Oct. 24-27, 2012 (Oral presentation).

Kohen, A.

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56. Mishanin, T.V., Kohen, A., “Toward better antibiotics: Studies of alternative thymidylate synthase”, Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 15-16, 2012. (Oral Presentation).

57. Abeysinghe, D.T., Wang, Z. and Kohen, A., Effect of Y209F on the hydride transfer step in thymidylate synthase”, Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 15-16, 2012.

58. Roston, D., Cheatum, C.M., Major, D.T., Kohen, A., “Two Complementary Approaches Illuminate the Hydride Transfer Catalyzed by Dihydrofolate Reductase”, Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 15-16, 2012.

59. Singh P., Kohen, A., “Global network in enzyme catalysis: Studies of dihydrofolate reductase”, CBB Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 15-16, 2012.

60. Koehn, E.M., Perissinotti, L.L., Moghram, S., Prabhakar, A., Lesley, S.A., Mathews, I.I.,* Kohen, A.,* “The folate binding site of flavin-dependent thymidylate synthase”, XXXII Midwest Enzyme Chemistry Conference”, University of Chicago, IL, Oct. 13, 2012. (Oral presentation).

61. Abeysinghe D.T., Wang, Z., Kohen, A., “Efects of a remote mutation on the hydride transfer in thymidylate synthase”, XXXII Midwest Enzyme Chemistry Conference”, University of Chicago, IL, Oct. 13, 2012.

62. Guo, Q., Cheatum, C.M., Kohen, A., “Mutant formate dehydrogenase studies reveal altered active site dynamics”, XXXII Midwest Enzyme Chemistry Conference”, University of Chicago, IL, Oct. 13, 2012.

63. Mishanina, T.V., Koehn, E.M., Conrad, J.A., Palfey, B., Lesley, S.A., Kohen, A., “Piecing together the mechanism of flavin-dependent thymidylate synthase”, XXXII Midwest Enzyme Chemistry Conference”, University of Chicago, IL, Oct. 13, 2012.

64. Pagano, P., Cheatum, C.M., Kohen, A., “Thedynamic nature of enzyme catalysis: Studies of dihydrofolate reductase”, XXXII Midwest Enzyme Chemistry Conference”, University of Chicago, IL, Oct. 13, 2012.

65. Roston, D., Cheatum, C.M., Major, D.T., Kohen, A., “Two complementary approaches illuminate the hydride transfer catalyzed by dihydrofolate reductase”, XXXII Midwest Enzyme Chemistry Conference”, University of Chicago, IL, Oct. 13, 2012.

66. Singh P., Kohen, A., “Global network in enzyme catalysis: Studies of dihydrofolate reductase”, XXXII Midwest Enzyme Chemistry Conference”, University of Chicago, IL, Oct. 13, 2012.

67. Stojkovic, V., Perissinotti, L.L., Gakhar, L., Benkovic, S.J., Kohen, A., “Explorations in enzymology: Role of the active site residues in the hydride transfer in dihydrofolate reductase”, XXXII Midwest Enzyme Chemistry Conference”, University of Chicago, IL, Oct. 13, 2012.

68. Wang, W., Ferrer, S., Moliner, V., and Kohen, A., “Protein Motions in Thymidylate Synthase Catalyzed Reactions: Experiments and Theories”, 14th Jakobsen Graduate Conference, Iowa City, March 23, 2012. (Oral presentation). 2nd Prize for Best Abstract.

69. Mishanina, T.V., Koehn, E.M., Kohen, A., "Trapping of an intermediate in a novel thymine biosynthesis pathway." 14th Jakobsen Graduate Conference, Iowa City, March 23, 2012. (Oral presentation).

70. Singh, P.N., Stojkovic, V., Kohen, A., “Exploring the Role of Global Networks in Enzymatic Catalysis through the Study of Distal Mutants of Dihydrofolate Reductase Using Primary Kinetic Isotope Effect”, 14th Jakobsen Graduate Conference, Iowa City, March 23, 2012.

71. Roston, D. Cheatum, C.M., Kohen, A.*, “Conformational Distributions at the Tunneling Ready State of H-Transfers”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences. Hotel Galvez in Galveston, Texas, Feb. 5-10, 2012.

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26

72. Wang, W., Ferrer, S., Moliner, V., and Kohen, A., “The Role Of Protein Motions In Thymidylate Synthase Catalyzed Reactions: A Hybrid Experimental - Theoretical Study”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences. Hotel Galvez in Galveston, Texas, Feb. 5-10, 2012.

73. Saeed, M., Nilaweera, T.D., Kohen, A.*, “Targeting thymidylate synthase activity and folate receptors to assess therapeutic response and cancer imaging by positron emission tomography (PET)”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences. Hotel Galvez in Galveston, Texas, Feb. 5-10, 2012.

74. Mishanina, T.V., Koehn, E.M., Kohen, A., “Flavin-dependent thymidylate synthase: trapping of a reaction intermediate”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences. Hotel Galvez in Galveston, Texas, Feb. 5-10, 2012.

75. Roston, D. Cheatum, C.M., Kohen, A.*, “Conformational Distributions at the Tunneling Ready State of H-Transfers”, Gordon Research Seminar on Isotopes in Biological and Chemical Sciences. Hotel Galvez in Galveston, Texas, Feb. 4-5, 2012. (Oral presentation).

76. Saeed, M., Nilaweera, T.D., Kohen, A.*, “Targeting thymidylate synthase activity and folate receptors to assess therapeutic response and cancer imaging by positron emission tomography (PET)”, Gordon Research Seminar on Isotopes in Biological and Chemical Sciences. Hotel Galvez in Galveston, Texas, Feb. 4-5, 2012. (Oral presentation).

77. Wang, W., Ferrer, S., Moliner, V., and Kohen, A., “The Role Of Protein Motions In Thymidylate Synthase Catalyzed Reactions: A Hybrid Experimental - Theoretical Study”, Gordon Research Seminar on Isotopes in Biological and Chemical Sciences. Hotel Galvez in Galveston, Texas, Feb. 4-5, 2012. (Oral presentation).

78. Mishanina, T.V., Koehn, E.M., Kohen, A., “Flavin-dependent thymidylate synthase: trapping of a reaction intermediate”, Gordon Research Seminar on Isotopes in Biological and Chemical Sciences. Hotel Galvez in Galveston, Texas, Feb. 4-5, 2012.

79. Guo, Q., Cheatum, C.M., Kohen, A., “Characterizing Fast Dynamics at the Active Site of Formate Dehydrogenase Using 3-Picolyl Azide Adenine Dinucleotide”, Southeastern Regional Meeting of the American Chemical Society, Richmond, Virginia, Oct. 26-29, 2011. (Oral presentation).

80. Abeysinghe, T., Wang, Z., Kohen, A., “Effect of a mutant With altered dynamics on the hydride transfers catalyzed by Thymidylate Synthase”, The 46th Midwest / 39th Great Lakes Regional meetings of American Chemical Society (ACS), Saint Louis, MO, Oct. 19-22, 2011.

81. Guo, Q., Cheatum, C.M., Kohen, A., “Synthesis of picolyl azide analogues of nicotinamide as potential two-dimensional infrared probes of NAD(P)-dependent enzyme dynamics”, 10th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 17-18, 2011.

82. Wang, W., Moliner, V., and Kohen, A., “The Role Of Enzyme Dynamics In Thymidylate Synthase Catalyzed Reaction: A Combined Experimental-Theoretical Approach”, 10th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 17-18, 2011.

83. Roston, D. Cheatum, C.M., Kohen, A.*, “Using Kinetic Isotope Effects to Assess Dynamic Motions of the Substrates in Enzymatic H-Transfers”, XXXI Midwest Enzyme Chemistry Conference”, University of Chicago, IL, Oct. 15, 2011. (Oral presentation).

84. Mishanina, T.V., Koehn, E.M., Conrad, J.A., Palfey, B.A., Lasley, S.A., Kohen, A.*, “Chemical trapping of an intermediate in the reaction catalyzed by flavin dependent thymidylate synthase”, XXXI Midwest Enzyme Chemistry Conference”, University of Chicago, IL, Oct. 15, 2011.

Kohen, A.

27

85. Singh, P., Francis, K., Kohen, A.* “Study of distal mutants of dihydrofolate reductase and exploring the role of global networks in enzymatic catalysis”, XXXI Midwest Enzyme Chemistry Conference”, University of Chicago, IL, Oct. 15, 2011.

86. Wang, Z., Moliner, V., Kohen, A.*, “The dynamics-function relationship in the thymidylate synthase catalyzed reaction: A hybrid experimental-theoretical study”, XXXI Midwest Enzyme Chemistry Conference”, University of Chicago, IL, Oct. 15, 2011.

87. Guo, Q., Pagano, P., Perissinotti, L.L., Cheatum, C.M.,* and Kohen, A.,* “Synthesis of Picolyl Azide Analogues of Nicotinamide as Potential Two-dimensional Infrared Probes of NAD(P)-dependent Enzyme Dynamics”, XXXI Midwest Enzyme Chemistry Conference”, University of Chicago, IL, Oct. 15, 2011.

88. Abeysinghe, T., Wang, Z., and Kohen, A.,* “Long range protein effects in catalysis of thymidylate synthase”, XXXI Midwest Enzyme Chemistry Conference”, University of Chicago, IL, Oct. 15, 2011.

89. Saeed, M., Nilaweera, T., Kohen, A.,*, “Targeting Human Thymidylate Synthase Enzyme for the Development of Positron Emission Tomography Tracer to Distinguish Normal from Cancer Cells”, XXXI Midwest Enzyme Chemistry Conference”, University of Chicago, IL, Oct. 15, 2011.

90. Stojkovic, V., Perissinotti, L.L., Lee, J., Benkovic, S.J., Kohen, A.*, “Correlated effects of the donor acceptor distance and its fluctuation on hydride transfer in the Dihydrofolate reductase catalyzed reaction”, 242nd ACS National Meeting that will be held in Denver, Colorado, Aug. 28 – Sept. 1, 2011.

91. Stojkovic, V., Perissinotti, L.L., Lee, J., Benkovic, S.J., Kohen, A.*, "Dihydrofolate reductase: A correlation between the donor-acceptor distance and its fluctuation to the catalyzed hydride transfer", 42nd Meeting of the ACS Central Region in Indianapolis, IN, June 8 - 10, 2011. (Oral presentation).

92. Koehn, E.M., Lesley, S.A., Mathews, I.I., Kohen, A., “Flavin-dependent thymidylate synthase”, 42nd Meeting of the ACS Central Region in Indianapolis, IN, June 8 - 10, 2011. (Oral presentation).

93. Wang, Z. Stroud, R.M., Moliner, V., Kohen, A.*, “Thymidilate synthase: Dynamics-functions relationship in enzyme-catalyzed reactions”, ISOTOPE 2011, Gréoux-les-Bains, France, June 20-24, 2011. (Oral presentation)

94. Roston, D. Cheatum, C.M., Kohen, A.*, “Kinetic isotope effects as a probe of donor-acceptor dynamics in enzyme-catalyzed H-transfers”, ISOTOPE 2011, Gréoux-les-Bains, France, June 20-24, 2011. (Oral presentation)

95. Stojkovic, V., Perissinotti, L.L., Lee, J., Benkovic, S.J., Kohen, A.*, “Dihydrofolate reductase: A correlation between the donor-acceptor distance and its fluctuation to the catalyzed hydride transfer”, 22 Enzyme Mechanism Conference, St. Pete Beach, FL, Jan 2-6, 2011.

96. Wang, Z., Kohen, A.*, “Thymidylate synthase catalyzed H-transfers: Role of tunneling and dynamics in different enzyme catalyzed C-H activations”, 22 Enzyme Mechanism Conference, St. Pete Beach, FL, Jan 2-6, 2011.

97. Koehn, E.M., Lesley, S.A., Mathews, I.I., Kohen, A., “Flavin-dependent thymidylate synthase: Studies towards the development of better antibiotics”, 22 Enzyme Mechanism Conference, St. Pete Beach, FL, Jan 2-6, 2011.

98. Mishanina, T.V., Kohen, A., “Hunting for an intermediate: Studies of chemical mechanism of flavin-dependent thymidylate synthase”, MWRM Wichita, IL, Oct. 28-29, 2010.

99. Wang, Z., Kohen, A., “Thymidylate Synthase Catalyzed H-Transfer: Two Chapters in One Tale”, 19th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 18-19, 2010. (Oral Presentation).

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28

100. Mishanina, T.V., Kohen, A., “Hunting for an Intermediate: Studies of the Chemical Mechanism of Flavin-Dependent Thymidylate Synthase”, 19th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 18-19, 2010.

101. Roston, D., Kohen, A., “Kinetic Isotope Effects as a Probe of Donor-Acceptor Dynamics in Enzyme Catalyzed H-Transfer”, 19th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 18-19, 2010.

102. Toraason, M., Wang, Z., Luzum, C., Kohen, A., “Studies on the Stability of N5,N10-Methylene-5,6,7,8- Tetrahydrofolate”, 19th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 18-19, 2010.

103. Yahashiri, A., Nixon, R. III, Hashmi, A., Manjunath, N., Toraason, M.J., Sen, A., Kohen, A., “Comparative Cross-Reactivity Kinetic Studies Of L. major Pteridine Reductase And E. coli Dihydrofolate Reductase”, 19th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 18-19, 2010.

104. Wang, Z., Kohen, A., “Thymidylate Synthase Catalyzed H-transfers: Two Chapters in One Tale”, XXX Midwest Enzyme Chemistry Conference, Northwestern University, Chicago, Illinois, Oct. 16, 2010. (Oral Presentation)

105. Koehn, E.M., Lesley, S.A., Mathews, I.I., Kohen, A., “Thymidlylate biosynthesis: Studies towards the development of better antibiotics”, XXX Midwest Enzyme Chemistry Conference, Northwestern University, Chicago, Illinois, Oct. 16, 2010.

106. Toraason, M., Wang, Z., Luzum, C., Kohen, A., “Studies on the Stability of N5,N10-Methylene-5,6,7,8- Tetrahydrofolate”, XXX Midwest Enzyme Chemistry Conference, Northwestern University, Chicago, Illinois, Oct. 16, 2010.

107. Stojković, V., Perissinoti, L.M., Kohen, A., “Linking Enzyme Dynamics To Catalysis: Studies Of DHFR Active Site Mutants”, XXX Midwest Enzyme Chemistry Conference, Northwestern University, Chicago, Illinois, Oct. 16, 2010.

108. Ditzler L., Sen, A., Gannon, M., Tivanski, A., Kohen, A., “Examination Of Dihydrofolate Reductase Activity On Gold Surfaces Via Molecular Force Recognition Spectroscopy”, XXX Midwest Enzyme Chemistry Conference, Northwestern University, Chicago, Illinois, Oct. 16, 2010.

109. Yahashiri, A., Toraason, M.J., Hashmi, A., Nixon, R. III, Manjunath, N., Sen, A., Kohen, A., “Comparative Cross-Reactivity Kinetic Studies Of Pteridine Reductase And Dihydrofolate Reductase”, XXX Midwest Enzyme Chemistry Conference, Northwestern University, Chicago, Illinois, Oct. 16, 2010.

110. Roston, D., Kohen, A., “Kinetic Isotope Effects As A Probe Of Donor-Acceptor Dynamics In Enzyme-Catalyzed H-Transfers”, XXX Midwest Enzyme Chemistry Conference, Northwestern University, Chicago, Illinois, Oct. 16, 2010.

111. Guo, Q., Chetume, C., Kohen, A., “Characterizing Fast Dynamics at the Active Site of Formate Dehydrogenase”, XXX Midwest Enzyme Chemistry Conference, Northwestern University, Chicago, Illinois, Oct. 16, 2010.

Kohen, A.

29

112. Mishanina, T.V., Kohen, A., “Hunting for an intermediate: Studies of chemical mechanism of flavin-dependent thymidylate synthase”, XXX Midwest Enzyme Chemistry Conference, Northwestern University, Chicago, Illinois, Oct. 16, 2010.

113. Abeysinghe, T.D., Wang., Z., Kohen, A., “Purification Of R-[6 XH] - N5, N10-Methylene-5, 6, 7, 8-Tetrahydrofolate for Studies Thymidylate Synthase”, XXX Midwest Enzyme Chemistry Conference, Northwestern University, Chicago, Illinois, Oct. 16, 2010.

114. Roston, D., Kohen, A., “Studies of Alcohol Dehydrogenase - Resolving an Old Paradox”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences. Hotel Galvez in Galveston, Texas, Feb. 14-19, 2010.

115. Wang, Z., Kohen, A., “Exploring the Dynamics-Kinetics Relationship: Kinetic Isotope Effect Studies of E. coli Thymidylate Synthase”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences. Hotel Galvez in Galveston, Texas, Feb. 14-19, 2010.

116. Stojkovic, V., Kohen, A., “Effect of Donor-Acceptor Distance on H-Tunneling in Enzyme Catalyzed Reaction: Studies of Active Site Mutants of DHFR”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences. Hotel Galvez in Galveston, Texas, Feb. 14-19, 2010.

117. Koehn, E.M., Fleischmann, T., Lesley, S.A., Mathews, I.I., Kohen, A., “An Unusual Flavin-Dependent Mechanism of Thymidylate Biosynthesis”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences. Hotel Galvez in Galveston, Texas, Feb. 14-19, 2010.

118. Sen, A., Kohen, A., “The effect of pressure on dihydrofolate reductase and a dynamically-altered mutant”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences. Hotel Galvez in Galveston, Texas, Feb. 14-19, 2010.

119. Jean-Gilles, E., Koehn, E.M., Kohen, A., “The Order of Substrates Binding in Enzymatic Reaction” Annual Biomedical Research Conference for Minority Students (ABRCMS) conference, Phoenix, Arizona, Nov. 4 -7, 2009.

120. Roston, D., Kohen, A., “Studies of Alcohol Dehydrogenase - Resolving an Old Paradox”, 18th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 19-20, 2009. (Oral Presentation)

121. Wang, Z., Kohen, A., “Exploring the Dynamics-Kinetics Relationship: Kinetic Isotope Effect Studies of E. coli Thymidylate Synthase”, 18th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 19-20, 2009.

122. Luzum, C., Wang, Z., Kohen, A., “The Stability of N5,N10-Methylene-5,6,7,8-Tetrahydrofolate under Physiological Conditions”, 18th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 19-20, 2009.

123. Mishanina, T.V., Koehn, E.M., Jean-Gilles, E., Wang, Z., Kohen, A., “The Inhibition of Oxidase Activity of Flavin-Dependent Thymidylate Synthase by the Substrate CH2H4folate”, 18th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 19-20, 2009.

124. Yahashiri, A., Hashmi, A., Kohen, A., “Comparison of the Enzymatic H-transfer Reactions of Leishmania major Pteridine Reductase 1 and E. coli Dihydrofolate Reductase”, 18th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 19-20, 2009.

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125. Yahashiri, A., Jo, H., Nimrod, G., Ben-Tal, N, Howell, E.E., Kohen, A., “Variation of Isotopic Labeling of NADPH and their Application in the Assessment of Accelerated H transfer in Primitive R67 Dihydrofolate reductase at high ionic strength”, 18th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 19-20, 2009.

126. Dutta, S., Cheatum, C.M., Kohen, A., A New Mid-IR Probe to Investigate Ultrafast Dynamics of Enzymes”, Midwest Regional Meeting of the ACS (MWRM 2009), Iowa City, IA, Oct. 21-24, 2009.

127. Roston, D., Kohen, A., “The Elusive Transition State of Alcohol Dehydrogenase Unveiled”, Midwest Regional Meeting of the ACS (MWRM 2009), Iowa City, IA, Oct. 21-24, 2009. (Oral Presentation)

128. Stojkovic, V., Kohen, A., “Effect of Donor-Acceptor Distance on H-Tunneling in Enzyme Catalyzed Reaction: Studies of Active Site Mutants of DHFR”, Midwest Regional Meeting of the ACS (MWRM 2009), Iowa City, IA, Oct. 21-24, 2009. (Oral Presentation)

129. Koehn, E.M., Fleischmann, T., Lesley, S.A., Mathews, I.I., Kohen, A., “An Unusual Flavin-Dependent Mechanism of Thymidylate Biosynthesis”, Midwest Regional Meeting of the ACS (MWRM 2009), Iowa City, IA, Oct. 21-24, 2009. (Oral Presentation)

130. Sen, A., Kohen, A., “The effect of pressure on dihydrofolate reductase and a dynamically-altered mutant”, Midwest Regional Meeting of the ACS (MWRM 2009), Iowa City, IA, Oct. 21-24, 2009. (Oral Presentation)

131. Wang, Z., Kohen, A., “Exploring the Role of Dynamics in Enzymatic Catalysis: Kinetic Isotope Effect Studies of E. coli Thymidylate Synthase”. Midwest Regional Meeting of the ACS (MWRM 2009), Iowa City, IA, Oct. 21-24, 2009. (Oral Presentation)

132. Toraason, Wang, Z., M., Sheff, D., Kohen, A., “Studies On the Stability of [11-14C]-N5,N10-methylene-5,6,7,8-tetrahydrofolate Under Physiological Conditions”, Midwest Regional Meeting of the ACS (MWRM 2009), Iowa City, IA, Oct. 21-24, 2009.

133. Yahashiri, A., Toraason, M., Kohen, A., “Effective purification and kinetic studies of Pteridine Reductase 1”, Midwest Regional Meeting of the ACS (MWRM 2009), Iowa City, IA, Oct. 21-24, 2009.

134. Yahashiri, A., A., Kohen, A., “Studies of Hydrogen Transfer by E. Coli Chromosomal DHFR on an unnatural substrate”, Midwest Regional Meeting of the ACS (MWRM 2009), Iowa City, IA, Oct. 21-24, 2009.

135. Stojkovic, V., Kohen, A., “Contribution of Active site Dynamics to Enzyme Catalysis: Study on a Series of Active Site Mutants within Dihydrofolate Reductase (DHFR) from E.coli”, XXIX Midwest Enzyme Chemistry Conference, Loyola University, Chicago, Illinois, Oct. 10, 2009. (Oral Presentation)

136. Koehn, E.M., Fleischmann, T., Lesley, S.A., Mathews, I.I., Kohen, A., “An Unusual Flavin-Dependent Mechanism of Thymidylate Biosynthesis”, XXIX Midwest Enzyme Chemistry Conference, Loyola University, Chicago, Illinois, Oct. 10, 2009 (Oral presentation).

137. Sen, A., Hay, S., Scrutton, N. Kohen, A., “Pressure dependence of the hydride-transfer step in dihydrofolate reductase and a dynamically altered double mutant”, XXIX Midwest Enzyme Chemistry Conference, Loyola University, Chicago, Illinois, Oct. 10, 2009.

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138. Roston, D., Kohen, A., “Transition State Structure from Anomalous Kinetic Isotope Effects: Experiment and Theory”, XXIX Midwest Enzyme Chemistry Conference, Loyola University, Chicago, Illinois, Oct. 10, 2009.

139. Wang, Z., Kohen, A., “Probing the Dynamics-Kinetics relationship: Kinetic Isotope Effect Studies of E. coli Thymidylate Synthase”, XXIX Midwest Enzyme Chemistry Conference, Loyola University, Chicago, Illinois, Oct. 10, 2009.

140. Luzum, C., Wang, Z., Kohen, A., “The Stability of N5,N10-methylene-5,6,7,8-tetrahydrofolate under Physiological Conditions”, XXIX Midwest Enzyme Chemistry Conference, Loyola University, Chicago, Illinois, Oct. 10, 2009.

141. Mishanina, T.V., Jean- Glles, E., Koehn, E.M., Kohen, A., “Exploring the Mechanism of Flavin-Dependent Thymidylate Synthase”, XXIX Midwest Enzyme Chemistry Conference, Loyola University, Chicago, Illinois, Oct. 10, 2009.

142. Yahashiri, A., Hashmi, A., Toraason Kohen, A., “Investigation of H-transfer Reaction with E. coli Dihydrofolate Reductase and L. major Pteridine Reductase 1. ”, XXIX Midwest Enzyme Chemistry Conference, Loyola University, Chicago, Illinois, Oct. 10, 2009.

143. Hashmi, A., Toraason, M., Yahashiri, A., Kohen, A., “Leishmania Major Pteridine Reductase 1 Purification by Use of Methotrexate-Agarose Affinity Chromatography”, XXIX Midwest Enzyme Chemistry Conference, Loyola University, Chicago, Illinois, Oct. 10, 2009.

144. Koehn, J., Koehn, E.M., Kohen, A., “Synthesis of a Novel Intermediate of Thymidylate Biosynthesis”, Summer Undergraduate Research Conference, UI, July 30, 2009.

145. Jean-Gilles, E., Koehn, E.M., Wang, Z., Kohen, A., “Inhibition of the Oxidase Activity of Flavin –Dependent Thymidylate Synthase by the Substrate CH2H4folate”, Summer Undergraduate Research Conference, UI, July 30, 2009.

146. Hashmi, A., Toraason, M., Yahashiri, A., Kohen, A., “PTR1 Enzyme Purification”, Summer Undergraduate Research Conference, UI, July 30, 2009.

147. Koehn, E.M., Fleischmann, T., Lesley, S.A., Mathews, I.I., Kohen, A., “Thymine Biosynthesis in Organisms Containing the thyX Gene” Gordon Research Conference on Nucleosides, Nucleotides, and Oligonucleotides (GRC - N2O), Salve Regina University, Newport, RI, July 5-10, 2009. This is an invited talk and since Kohen could not attend the meeting Koehn presented this talk.

148. Yahashiri, A., Howell, E.E., Kohen, A., “Has Evolution Tuned Enzyme Dynamics and H-Tunneling?”, 21st Enzyme Mechanisms Conference, Tucson, AZ, Jan. 3-6, 2008.

149. Koehn, E.M., Fleischmann, T., Lesley, S.A., Mathews, I.I., Kohen, A., “A Novel Chemical Mechanism

of Thymine Biosynthesis in Organisms Containing the thyX Gene”, 21st Enzyme Mechanisms Conference, Tucson, AZ, Jan. 3-6, 2008.

150. Koehn, E.M., Fleischmann, T., Lesley, S.A., Mathews, I.I., Kohen, A., “Chemistry of Thymidylate

Synthesis in Organisms Containing the thyX Gene”, 17th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 20-21, 2008.

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151. Roston, D., Kohen, A., “Rewinding Yeast Alcohol Dehydrogenase: Tunneling and Coupled Motion in Reverse”, 17th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 20-21, 2008.

152. Stojković, V., Kohen, A., “Use of Kinetic Isotope Effects to Study the Active Site Dynamics of

Dihydrofolate Reductase (DHFR)”, 17th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 20-21, 2008.

153. Wang, Z., Chernyshev, A., Koehn, E.M., Manuel, A., Lesley, S.A., Kohen, A., “Studies of the Oxidase

Activity of a Flavin-Dependent Thymidylate Synthase Reveal Features of the Synthase Activity”, 17th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct 20-21, 2008.

154. Yahashiri, A., Howell, E.E., Kohen, A., “Proof of Accelerated H-Transfer with Primitive R67

Dihydrofolate Reductase at High Ionic Strength”, 17th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 20-21, 2008.

155. Jo, H., Yahashiri, A., Stojković, V., Kohen, A., “One-Step Chemo-Enzymatic Micro-Synthesis of

Reduced β-nicotinamide Adenine Dinucleotide 2’-Phosphate”, 17th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 20-21, 2008.

156. Banderia, J.N., Dutta, S., Nydegger, M.W., Rock, W.T., Cheatum, C.M., Kohen, A., “Comparison of

Kinetics and Dynamics of Formate Dehydrogenase Using Isotope Effects and Ultrafast Spectroscopy”, 17th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 20-21, 2008.

157. Stojković, V., Kohen, A., “Kinetic Isotope Effect Studies of Dihydrofolate Reductase (DHFR): The

Role of Local Alteration of the Active Site Environment in Catalysis”, XXVIII Midwest Enzyme Chemistry Conference, Chicago, Illinois, Oct. 4, 2008.

158. Roston, D., Kohen, A., “Tunneling and Coupled Motion in Yeast Alcohol Dehydrogenase”, XXVIII

Midwest Enzyme Chemistry Conference, Chicago, Illinois, Oct. 4, 2008.

159. Jo, H., Yahashiri, A., Stojković, V., Kohen, A., “Chemo-Enzymatic Synthesis of Biologically Important Reagents”, XXVIII Midwest Enzyme Chemistry Conference, Chicago, Illinois, Oct. 4, 2008.

160. Wang, Z., Chernyshev, A., Koehn, E.M., Manuel, A., Lesley, S.A., Kohen, A., “Oxidase Activity of

Flavin-Dependent Thymidylate Synthase”, XXVIII Midwest Enzyme Chemistry Conference, Chicago, Illinois, Oct. 4, 2008.

161. Wang, Z., Stroud, R.M., Kohen, A., “Probing the Dynamics-Kinetics Relationship: Studies on E. coli

Thymidylate Synthase and Its Mutants Distant from the Active Site”, XXVIII Midwest Enzyme Chemistry Conference, Chicago, Illinois, Oct. 4, 2008.

162. Koehn, E.M., Fleischmann, T., Lesley, S.A., Mathews, I.I., Kohen, A., “Determining the Chemical

Cascade of the Reaction Catalyzed by Flavin-Dependent Thymidylate Synthase“, XXVIII Midwest Enzyme Chemistry Conference, Chicago, Illinois, Oct. 4, 2008.

163. Yahashiri, A., Howell, E.E., Kohen, A., “Accelerated H Transfer of Primitive R67 Dihydrofolate

Reductase at High Ionic Strength”, XXVIII Midwest Enzyme Chemistry Conference, Chicago, Illinois, Oct. 4, 2008.

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164. Banderia, J.N., Dutta, S., Nydegger, M.W., Rock, W.T., Cheatum, C.M., Kohen, A., “Study of Kinetic Isotope Effect and Photon Echoes in Formate Dehydrogenase”, XXVIII Midwest Enzyme Chemistry Conference, Chicago, Illinois, Oct. 4, 2008.

165. Sen, A., Kohen, A., “Exploring the Role of Global Networks in Enzymatic Catalysis: Further Studies

with Dynamically Altered Mutants of Dihydrofolate Reductase”, XXVIII Midwest Enzyme Chemistry Conference, Chicago, Illinois, Oct. 4, 2008.

166. Cheatum, C.M., Bandaria, J.N., Hill, S.E., Dutta, S., Kohen, A., “Watching the Protein Mambo:

Spectroscopic Probes of Enzyme Dynamics”, 235th ACS National Meeting, New Orleans, LA, Apr. 6-10, 2008. (Oral)

167. Kohen, A., Yahashiri, A., Howell, E.E., “The Role of Evolution in Tuning Environmentally Coupled

Tunneling in Enzymes”. 235th ACS National Meeting, New Orleans, LA, Apr. 6-10, 2008.

168. Yahashiri, A., Howell, E.E., Kohen, A., “Tuning of the H-Transfer Coordinate in Primitive vs. Well-Evolved Enzymes”, Gordon Research Conferences: Isotopes In Biological & Chemical Sciences, Ventura, CA, Feb. 17-21, 2008. [Included in the ACS Posters2View™ database].

169. Fleischmann, T., Koehn, E., Kohen A., “Probing the Chemistry of Flavin-Dependant Thymidylate

Synthase”, American Chemical Society - Midwest Regional Meeting, Nov. 7 -10, 2007. [Oral presentation]

170. Sen, A., and Kohen, A., “Secondary Kinetic Isotope Effects Probing the Chemical Step in DHFR”,

American Chemical Society - Midwest Regional Meeting, Nov. 7 -10, 2007. [Oral presentation] 171. Sen, A., and Kohen, A., “Isotopic Labeling of Nicotinamide Co-factors for the Measurement of H/D

Kinetic Isotope Effects (KIEs) in Enzymes”, XXVII Midwest Enzyme Chemistry Conference, Chicago, Illinois, Sept. 29, 2007.

172. Sharma, V., Hong., B., and Kohen, A., “Temperature Dependence on the Proton Abstraction Step in the

Catalysis by Escherichia coli Thymidylate Synthase”, XXVII Midwest Enzyme Chemistry Conference, Chicago, Illinois, Sept. 29, 2007.

173. Fleischmann, T., Koehn, E., Kohen A., “Probing a Chemical Mechanism for FDTS”, XXVII Midwest

Enzyme Chemistry Conference, Chicago, Illinois, Sept. 29, 2007. 174. Yahashiri, A., Howell, E.E., and Kohen, A., “Evolutionary Prospective on Environmentally Coupled

Tunneling: Comparison of the Nature of Hydrogen Transfer between E. Coli Chromosomal DHFR and Plasmid-Coded R67 DHFR”, XXVII Midwest Enzyme Chemistry Conference, Chicago, Illinois, Sept. 29, 2007.

175. Wang, Z., Chernyshev, A., Fleischmann, T., and Kohen, A., “The Competition between O2 and 5,10-

Methylene-5,6,7,8-Tetrahydrofolate in the Oxidative Half Reactions of Flavin-Dependent Thymidylate Synthase”, XXVII Midwest Enzyme Chemistry Conference, Chicago, Illinois, Sept. 29, 2007.

176. Wang, Z., Chernyshev, A., Fleischmann, T., and Kohen, A. “The Competition between O2 and 5,10-

Methylene- 5,6,7,8-Tetrahydrofolate (CH2THF) in the Oxidative Half Reaction of Flavin-Dependent Thymidylate Synthase (FDTS)”, Central States Society of Toxicology Annual Meeting, Iowa City, IA, Sept. 20-21, 2007.

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177. Yahshiri A., Howell, E.E., and Kohen, a. “Nature of hydrogen transfer in R67 dihydrofolate reductase catalysis: evolutionary prospective on environmentally coupled tunneling ”, Central States Society of Toxicology Annual Meeting, Iowa City, IA, Sept. 20-21, 2007.

178. Haddad, M. and Kohen, A., “The study of flavin-dependent thymidylate synthase in pathogenic

organisms”, Annual Biomedical Research Conference for Minority Students (ABRCMS), Anaheim, CA, Nov. 8 - 11, 2006. Abstract published in FASEB J. 2007; 21:lb121

179. Wang, L., Goodday, N.M., Benkovic, S. J., and Kohen, A. ”Coordinated effects of distal mutations on

environmentally coupled tunneling in dihydrofolate reductase”, XXVI Midwest Enzyme Chemistry Conference, Chicago, Illinois, Sept. 29, 2006 (oral presentation).

180. Sen, A, Haddad, M., and Kohen, A. ”2˚ KIE studies of dihydrofolate reductase and its distal mutants”,

XXVI Midwest Enzyme Chemistry Conference, Chicago, Illinois, Sept. 29, 2006. 181. Chernyshev, A., Fleischmann, T., and Kohen, A., “Flavin-dependent thymidylate synthase dual

functionality”, XXVI Midwest Enzyme Chemistry Conference, Chicago, Illinois, Sept. 29, 2006. 182. Haddad, M. and Kohen, A., “Flavin-dependent thymidylate synthase”, 7th Research Symposium, Iowa

Biosciences Advantage, July 28, 2006 (oral presentation). 183. Haddad, M. and Kohen, A., “2˚ KIEs as probe of coupled motion in DHFR”, 37th ACS Great Lakes

Regional Meeting, Milwaukee, IL, May 30, 2006. 184. Wang, L., Antikainen, N., Benkovic, S. J., and Kohen, A., “The effects of altered dynamics on the chemical

step of dihydrofolate reductase”, Gordon Research Conferences: Isotopes In Biological & Chemical Sciences, Ventura, CA, Feb.17-21, 2006 (oral presentation).

185. Hong, B., Agrawal, N., and Kohen, A., “H-Tunneling with Y94F E. coli thymidylate synthase”, 25th

Annual Midwest Enzyme Chemistry Conference, Chicago, IL, USA, Oct. 8, 2005. 186. Bandaria, J.N., Gundogdu, C., Hill, S., Cheatum, C., and Kohen, A., “Two dimensional infrared

spectroscopy as a new tool in Enzymology”, 25th Annual Midwest Enzyme Chemistry Conference, Chicago, IL, USA, Oct. 8, 2005.

187. Wang, L., Antikainen, N., Benkovic, S. J., and Kohen, A., “The Effects of Altered Dynamics on the Chemical

Step of Dihydrofolate Reductase”, 25th Annual Midwest Enzyme Chemistry Conference, Chicago, IL, USA, Oct. 8, 2005.

188. N. Agrawal, and A. Kohen, “Comparison of flavin-dependent and classical thymidylate synthases”, ASBMB,

Experimental Biology Meeting, San Diego, CA, Apr. 2-6, 2005. 189. N. Agrawal, and A. Kohen, “An alternative flavin-dependent mechanism for biosynthesis of thymidylate

synthase”, AAAS Annual Meeting, Washington, DC, Feb. 17-21, 2005, (oral presentation). 190. L. Wang, S. Tharp, T. Selzer, S.J. Benkovic, and A. Kohen, “The Effects of Altered Dynamics on the Chemical

Step of Dihydrofolate Reductase” 14th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 24-26, 2005, (oral presentation).

191. L. Wang, S. Tharp, T. Selzer, S.J. Benkovic, and A. Kohen, “Enzyme Dynamics and H-tunneling with DHFR”,

19th Enzyme Mechanisms Conference, Asilomar, Pacific Grove, CA, Jan. 5-9, 2005.

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192. B. Hong, N. Agrawal, C. Mihai, and A. Kohen, “Mutational studies of thymidylate synthase”, 19th Enzyme

Mechanisms Conference, Asilomar, Pacific Grove, CA, Jan. 5-9, 2005.

193. N. Agrawal, and A. Kohen, “An alternative flavin-dependent mechanism for biosynthesis of thymidylate, one of the four building blocks of DNA”, 36th ACS Great Lakes Regional Meeting 2004, Peoria, IL, Oct.17-20, 2004.

194. N. Agrawal, and A. Kohen, “Mechanistic studies of a flavin-dependent thymidylate synthase”, Northeast

Regional ACS Meeting at Rochester, Rochester NY, Oct. 31- Nov. 3, 2004. 195. Wang, L., Thrap, S., Selzer, T., Benkovic, S. J., and Kohen, A., “Comparison of H-transfer between the wild

type E. coli dihydrofolate reductase and its G121V mutant”, 24th Annual Midwest Enzyme Chemistry Conference, Chicago, IL, USA, Oct. 9, 2004.

196. A.V. Chernyshev, A.K. Sra, M.W. Ribb, D.R. Dean, and A. Kohen, “KIE studies of the enzyme nitrogenase”,

24th Annual Midwest Enzyme Chemistry Conference, Chicago, IL, USA, Oct. 9, 2004. 197. N. Agrawal, B. Hong, C. Mihai, L. Wang, R.S. Sikorski, and A. Kohen, “Tunneling and dynamics studies with

dihydrofolate reductase and thymidylate synthase”, Gordon Research Conference on Enzymes, Cofactors, and Metabolic Pathways, Kimball Union Academy, Meriden, NH, July 18-23, 2004.

198. N. Agrawal, and A. Kohen, “Mechanistic studies of enzymes in aqueous buffers”, Gordon Research Conference

on Green Chemistry, Roger Williams University, Bristol, RI, July 4-9, 2004. 199. A.V. Chernyshev, A.K. Sra, M.W. Ribb, D.R. Dean, and A. Kohen, “Competitive 15N kinetic isotope effects of

nitrogenase catalyzed dinitrogen reduction”, Gordon Research Conference on Nitrogen Fixation, Colby-Sawyer, New London, NH, June 20-25, 2004.

200. N. Agrawal, B. Hong, C. Mihai, L. Wang, R.S. Sikorski, and A. Kohen (also oral presentation),

“Environmentally coupled tunneling in enzymatic hydride transfer reactions”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences, Holiday Inn, Ventura, CA, Feb. 15-20, 2004.

201. A.K. Sra, A.V. Chernyshev, M.W. Ribb, D.R. Dean, and A. Kohen, “15N V/K kinetic isotope effects of N2

reduction by nitrogenase”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences, Holiday Inn, Ventura, CA, Feb. 15-20, 2004.

202. L. Wang, R.S. Sikorski, and A. Kohen, “V/K kinetic isotope effects (KIE) with dihydrofolate reductase”, 23rd

Annual Midwest Enzyme Chemistry Conference, Chicago, IL, Oct. 4, 2003. 203. A.K. Sra, A.V. Chernyshev, M.W. Ribb, D.R. Dean, and A. Kohen, “Competitive 15N kinetic isotope effects of

nitrogenase catalyzed dinitrogen reduction”, 23rd Annual Midwest Enzyme Chemistry Conference, Chicago, IL, Oct. 4, 2003.

204. N. Agrawal, B. Hong, C. Mihai, and A. Kohen, “Enzyme dynamics and quantum mechanical tunneling in w.t.

E.coli thymidylate synthase”, 23rd Annual Midwest Enzyme Chemistry Conference, Chicago, IL, Oct. 4, 2003. 205. K.A. Markham and A. Kohen, “Mechanistic studies of the hydride transfer step in bovine liver glutamate

dehydrogenase”, 23rd Annual Midwest Enzyme Chemistry Conference, Chicago, IL, Oct. 4, 2003.

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206. N. Agrawal, C. Mihai, and A. Kohen, “Temperature dependence studies of kinetic isotope effects with w.t. E. coli thymidylate synthase”, International Isotope effects Conference, an EUCHEM conference, Uppsala, Sweden, June 22-27, 2003.

207. R.S. Sikorski, K.A. Markham, L. Wang and A. Kohen, “V/K kinetic isotope effects (KIE) with dihydrofolate

reductase”, International Isotope effects Conference, an EUCHEM conference, Uppsala, Sweden, June 22-27, 2003. (Oral presentation)

208. N. Agrawal and A. Kohen, “Exploring the mechanism of thymidylate synthase using kinetic isotope effects”,

18th Enzyme Mechanisms Conference, San Luis Resort, Galveston Island, TX, Jan. 4-7, 2003. 209. K.A. Markham, R. S. Sikorski. L. Wang and A. Kohen, “Mechanistic studies using V/K KIEs for wt E. coli

dihydrofolate reductase”, 18th Enzyme Mechanisms Conference, San Luis Resort, Galveston Island, TX, Jan. 4-7, 2003.

210. N. Agrawal and A. Kohen, “Mechanistic studies of thymidylate synthase using kinetic isotope effects”, 11th

Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 22, 2002. 211. K.A. Markham and A. Kohen, “Primary and Secondary H/T KIE Studies with wt E. coli Dihydrofolate

Reductase”, 11th Biocatalysis and Bioprocessing Conference, Iowa City, IA, Oct. 22, 2002. 212. N. Agrawal and A. Kohen, “Mechanistic Studies of Thymidylate Synthase using Kinetic Isotope Effects”, 22nd

Midwest Enzyme Chemistry Conference, Chicago, IL, Sept. 28, 2002. 213. K.A. Markham and A. Kohen, “Primary and secondary H/T KIE studies with wt E. coli dihydrofolate

reductase”, 22nd Midwest Enzyme Chemistry Conference, Chicago, IL, Sept. 28, 2002. 214. A.K. Sra, and A. Kohen (also oral presentation), “15N studies of N2 reduction by nitrogenase”, Gordon Research

Conference on Nitrogen Fixation. Colby Sawyer College New London, NH, June 30-July 5, 2002. 215. A. Kohen (also oral presentation), K.A. Markham, J.A. McCracken, and M.S. Moore, “Studying the chemical

step in enzyme catalysis”, 223rd ACS National Meeting, Orlando, FL, Apr. 7-11, 2002. 216. M.S. Moore and A. Kohen, “Synthesis of 2H, 3H, and 14C labeled NADPH with low 1H contamination”, Gordon

Research Conference on Isotopes in Biological and Chemical Sciences. Holiday Inn, Ventura, CA, Feb. 18-22, 2002.

217. J.A. McCracken, K.A. Markham, and A. Kohen, “Determination of kinetic isotope effects on V/K with

dihydrofolate reductase”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences. Holiday Inn, Ventura, CA, Feb. 18-22, 2002.

218. K.A. Markham, J.A. McCracken, and A. Kohen, “Synthesis, purification and preservation of NADPH labeled

with 14C and 3H”, Gordon Research Conference on Isotopes in Biological and Chemical Sciences. Holiday Inn, Ventura, CA, Feb. 18-22, 2002.

219. A.K. Sra, and A Kohen, “Nitrogenase catalyzed dinitrogen reduction – a new mechanistic approach”, 21st

Midwest Enzyme Chemistry Conference, Chicago, IL, Oct. 13, 2001. 220. J.A. McCracken, M.S. Moore, K.A. Markham, and A Kohen, “Synthesis and storage of isotopically labeled

NADPH”, 21st Midwest Enzyme Chemistry Conference, Chicago, IL, Oct. 13, 2001.

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221. A.K. Sra, and A Kohen, “Nitrogenase catalyzed dinitrogen reduction – a new mechanistic approach”, 13th

International Congress on Nitrogen Fixation, Hamilton, Ontario, Canada, July 2-7, 2001.

Poster presentations of work performed prior to the appointment at Iowa: 1. A. Kohen, R. Cannio, S. Bartolucci, and J.P. Klinman, “What Role May Protein Dynamics Play in Enzyme

Catalysis? Hydrogen Tunneling Probes a Mechanistic Phase-Transition with Thermophilic Dehydrogenase”, Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways. Kimball Union Academy, Meriden, NH, July 12-17, 1998.

2. A. Kohen, R. Cannio, S. Bartolucci, and J.P. Klinman, “Beyond The Classics: Hydrogen Tunneling in Enzyme

Reaction”, The 16th Annual Meeting of the Biophysical Society. Kansas City, Missouri, Feb. 22-26, 1998. 3. A. Kohen, R. Cannio, S. Bartolucci, and J.P. Klinman, “Kinetic Isotope Effect Studies of Thermophilic

Dehydrogenase: Hydrogen Tunneling at High and Low Temperature”, Gordon Research Conference on Isotopes in Biology and Chemistry. Ventura, CA, Jan. 11-16, 1998.

4. A. Kohen, R. Cannio, S. Bartolucci, and J.P. Klinman, “Thermophilic Dehydrogenase: Hydrogen Tunneling at

High and Low Temperature”, Seventeenth International Conference of Biochemistry and Molecular Biology/1997 Annual Meeting of the American Society of Biology and Molecular Biology, San Francisco, CA, Aug. 24-29, 1997 [The FASEB J. 11, 1617 (1997)].

5. A. Kohen, R. Cannio, S. Bartolucci, and J.P. Klinman, “Isotope Effect Studies of a Thermophilic Enzyme”, 15th

Enzyme Mechanisms Conference. Naples, FL, Jan. 4-8, 1997. 6. A. Kohen, T. Jonsson and J.P. Klinman, “Glycoforms Modify Hydrogen Tunneling and Enthalpy of Activation:

A Possible Probe for the Effect of Protein Dynamics on Catalysis”, 10th Symposium of the Protein Society. San Jose, CA, Aug. 3-7, 1996.

7. A. Kohen, T. Jonsson and J.P. Klinman, “Glycosylation Modify Hydrogen Tunneling and Enthalpy of

Activation: A Possible Probe for the Effect of Protein Dynamics on Enzyme Catalysis”, Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways. Kimball Union Academy, Meriden, NH, July 14-19, 1996.

8. A. Kohen, T. Jonsson and J.P. Klinman, “Correlation Between Glucose Oxidase Mass, Hydrogen Tunneling

and Enthalpy of Activation: A Possible Probe for Protein Dynamics Effect on Catalysis”, Gordon Research Conference on Isotopes in Biology and Chemistry. Ventura, California, Feb. 11-16, 1996.

9. T. Baasov, A. Kohen, S. Sheffer-Dee-Noor and S. Du, “Towards the Mechanism-Based Inhibitors of KDO8P

Synthase - A Key Enzyme in the Biosynthesis of Lipopolysaccharides”, The 61st Annual Meeting of the Israel Chemical Society. The Hebrew University, Jerusalem, Israel, Feb. 13-14, 1996.

10. A. Kohen (also oral presentation) and T. Baasov, “Insight into the Catalytic Mechanism of KDO8P Synthase -

A Key Enzyme in the Biosynthesis of Lipopolysaccharides”, The Annual Meeting of the Israel Biochemical Society. Bar Ilan University, Ramat-Gan, Israel, Mar. 21-22, 1994.

11. A. Kohen, R. Berkovitch and T. Baasov, “Stereochemistry of KDO8P Synthase Catalyzed Reaction”, Fourth

European Symposium on Organic Reactivity; 2nd Newcastle Meeting on Molecular Mechanisms in Bioorganic Processes. "Organic Reactivity: Physical and Biological Aspects", Newcastle Upon Tyne, U.K., July 11-16, 1993.

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12. A. Kohen , R. Berkovitch, V. Belakhov and T. Baasov, “Stereochemistry of KDO8P Synthase Catalyzed

Reaction”, The 58th Annual Meeting of the Israel Chemical Society. Bar-Ilan University, Israel, Feb. 17-18, 1993.

13. A. Kohen and T. Baasov, “Steady State Kinetic Studies on 3-deoxy-D-manno-octulosonate-8-phosphate

Synthase from Escherichia coli”, The 57th Annual Meeting of the Israel Chemical Society. Technion - Israel Institute of Technology, Haifa, Israel, Feb. 12-13, 1992.

14. A. Jakob, A. Kohen and T. Baasov, “Mechanistic Studies on KDO8P Synthase. The Geometry of Substrate

Binding and Product Release”, The 57th Annual Meeting of the Israel Chemical Society. Technion - Israel Institute of Technology, Haifa, Israel, Feb. 12-13, 1992.

15. T. Baasov, A. Jakob and A. Kohen, “Mechanistic Studies on the Enzymes of Lipopolysaccharide Biosynthesis”,

The 56th Annual Meeting of the Israel Chemical Society. The Hebrew University, Jerusalem, Israel, Feb. 11-12, 1991.

16. E. Magal, J. C. Louis, A. Kohen and E. Yavin, “Brain Protein Kinase C in the Fetal Rat After Global

Ischaemia”, Second international symposium on pharmacology of cerebral ischaemia. University of Marburg, Marburg, Federal Republic of Germany, Oct. 3-5, 1988.

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TEACHING AT THE UNIVERSITY OF IOWA (cont. next page)

The courses Research in Chemistry (4:290, Grad. & 4:160, Undergrad.) and Research Seminar (4:291) are taught every semester and are not included in this table.

Semester/Year ADVISEES COURSES TAUGHT

Undrgrad Graduate Course No. and Title Enrollment

Fall 1999 0 0 4:225: Special Topics in Organic Chemistry 7

Spring 2000 1 1 4:142: Organic Lab for Chemistry Majors

4:285: Seminar Organic Chemistry 35

Fall 2000 2 1 4:285: Seminar Organic Chemistry 13 Spring 2001 2 3 4:142: Organic Lab for Chemistry Majors 29

Fall 2001 1 3 4:211: Chemical Catalysis in Biology 13 Spring 2002 3 4 4:142: Organic Lab for Chemistry Majors 32

Fall 2002 4 5 4:211: Chemical Catalysis in Biology 5 Spring 2003 3 6 4:142: Organic Lab for Chemistry Majors 29

Fall 2003 2 7 Flexible load Spring 2004 2 8 4:008: General Chemistry II 76

Fall 2004

2 8 4:225: Special Topics in Organic in Chemistry 4:121: Organic Chemistry Ia

4:285: Seminar Organic Chemistry

13 358 12

Spring 2005 2 8 4:008: General Chemistry II 73

Fall 2005 2 8 4:121: Organic Chemistry Ia

052:223: Introduction to Biocatalysisb 375 24

Spring 2006 4 9 4:008: General Chemistry II 75 Fall 2006 3 9 Career Development Award

Spring 2007 2 8 4:121: Organic Chemistry I

142:280 Topics in MCBb 302 17

Fall 2007

2 8 4:121: Organic Chemistry Ia 052:223: Introduction to Biocatalysisb

4:285: Seminar Organic Chemistry

274 15 18

Spring 2008 2 9 Scholars Award Fall 2008 2 9 4:121: Organic Chemistry Ia 390

Spring 2009 2 9 Scholars Award

128001 (Technion, Israel): Biological Catalysis

35

Fall 2009

3 7 4:121: Organic Chemistry I 4:029: First Year Seminar 052:223: Introduction to Biocatalysisc

382 15 13

Spring 2010 3 9 Scholars Award Fall 2010 3 9 4:121: Organic Chemistry Ia 394

Kohen, A.

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4:029: First Year Seminar 13 Spring 2011 2 10 4:142: Organic Lab for Majors 47

Fall 2011

2 10 4:029: First Year Seminar 052:223: Introduction to Biocatalysisc 4:191 Graduate Orientation 4:192 Ethics in Chemical Sciences

15 26 32 30

Spring 2012 3 11 4:008 General Chemistry II 274

Fall 2012

5 11 143:030 First Year Seminar (Honors) 4:191 Graduate Chemistry Orientation 4:192 Ethics in Chemical Sciences 4:604 Ethics in Chemical Sciences for Postdocs

16 39 40 17

Spring 2013 8 11 4:008 General Chemistry II 305

Fall 2013

9 11 052:223 Introduction to Biocatalysisc&d

143:030 First Year Seminar (Honors) 4:191 Graduate Chemistry Orientation 4:192 Ethics in Chemical Sciences 4:604 Ethics in Chemical Sciences for Postdocs

17 19 19 47 1

Spring 2014 8 11 4:121: Organic Chemistry I 373

Fall 2014

6 11 143:030 First Year Seminar (Honors) 4:191 Graduate Chemistry Orientation 4:192 Ethics in Chemical Sciences 4:604 Ethics in Chemical Sciences for Postdocs

18 28 28 5

a. One of two teachers. b. One of five teachers. c. One of four teachers. d. Director and Organizer.

Students and Postdoctoral Scholars Trained or in Training

Name Training Period Outcome Postdoctoral Associates

Amandeep Sra July 2000-Nov 2003 Research Scientist, UT Dallas, TX Steve Sikorski Aug 2002-Dec 2003 Director of Product Development, Garden of Life, FL Cornelia Mihai July 2002-August 2004 Associate Professor, NW Oklahoma State University Omar Bouh Jan 2003 Kelli Markham Jan 2005-June 2006 Chief of Nuclear Safety, Office of Nuclear Energy, DOE Anatoly Chernyshev Aug 2003-May 2008 Assist. Professor, University of Nizwa, Oman and Senior

Research & Development Consultant for Science at The Research Council of the Sultanate of Oman

Laura Perissinotti April 2010-March 2011 Postdoc. University of Calgary Muhammad Saeed Nov 2009 – May 2013 Assistant Professor, U. Malakand, Pakistan. Kevin Francis May 2011 - present

Kohen, A.

41

Graduate Students Kelli Markham Jan 2000 – Dec. 2004 Ph.D., Dec 04. Chief of Nuclear Safety, Office

of Nuclear Energy, DOE Malia Moore Jan 2001 – July 2002 M.S. May 2002; MD from U of Kansas Medical

School 2007 Jocelyn McCracken Jan 2001 – July 2002 M.S. May 2002; MBA 2005 Nitish Agrawal Jan 2002 – July 2005 Ph.D., Aug 2005. Postdoc - Scripps CBB Fellow Lin Wang June 2002 – Dec. 2006 CBB Fellow Ph.D., Dec 2006. Postdoc - Penn. State Baoyu Hong June 2003 – Sept. 2007 Ph.D., Jan. 2011. Scientist, ADDS

Pharmaceutals at Sunnyvale, CA Jigar Bandaria Jan 2004 – May 2009 Ph.D., July 2009. Postdoc - UC Berkeley CBB Fellow Todd Fleischmann Jan 2004 – Aug 2009 M.S. Aug 2009. Rockwell Labs Ltd., MI Atsushi Yahashiri Jan 2005 – June 2010 Ph.D., July 2010. Postdoc - UI Arundeti Sen Jan 2006 – Sept. 2011 Ph.D., Feb 2012. Research Associate in King's

College, London, UK Vanja Stojkovic Dec 2006 – Oct 2012 Ph.D., Oct. 2012. Postdoc at UCSF w/Fujimori Zhan Wang Jan 2007 – Dec 2012 Ph.D., Dec. 2012. Postdoc AECM w/ Schramm CBB Fellow; Founder's Award at Enzyme Mechanism

Conference; Jeremy Knowles Travel Award at the Enzymes GRC Daniel Roston Aug. 2007 – Aug. 2013 Ph.D., Aug. 2013. Postdoc UW Madison w/Cui NIH (T32) Fellow Eric Koehn Jan. 2008 – Aug. 2013 Ph.D., Aug. 2013. Postdoc UC Berkeley NSF Predoctoral Fellowship; CBB Fellow w/Klinman Tatiana Mishanina Jan 2009 – Aug. 2014 PhD, Aug. 2014, Postdoc U. Michigan CBB Fellow, McCloskey Award. w/Banergee

D. Thelma Abeysinghe Jan 2010 – Present Postcomp. Expected PhD July 2014 McCloskey Fellowship 2013 Priyanka N. Singh Jan 2010 – Present Postcomp. Expected PhD Aug. 2014 Qi (Gail) Guo Jan 2010 – Present Postcomp. McCloskey Fellowship 2012 Thushani Nilaweera Jan 2011 – July 2013 M.S. PhD student at U. Virginia. Philip Pagano Jan 2011 – Present Postcomp. Zahidul Islam Jan 2012 – Present Postcomp. CBB Fellow Ananda K. Ghosh Jan 2013 – Present Kalani U. Karunaratne Jan 2013 – Present CBB Fellow

Ilya Gurevic Jan 2014 – Present NIH (T32) Fellow

Kohen, A.

42

Chethya Ranasinghe Jan 2014 – Present Dibyendu Mondal June 2014 - Present

MSTP Rotation Student

Michael Choi June – July 2013 Undergraduates and Honors Students

Jeff Smith Jan 2000-Dec 2000 [Honors; U of IA, Chemistry 4:192] Thesis title: “HPLC Separations of DHFR Substrates and Nucleotides”.

Darren Berger Jan 2001-May 2001 [U of IA, Chemistry] Suzan Klaus Jan 2001-May 2001 [U of IA, Chemistry] Mike Klotz Aug 2001-Dec 2001 [U of IA, Chemistry] Lena Rydberg May 2002-Aug 2002 [U of IA, Biochemistry] Carla Asbury Feb 2002-July 2003 [U of IA, Chemistry] Elizabeth Vanderah May 2004-Aug 2004 [Iowa State U, Biochemistry] Jennifer Dibbern May 2004-Aug 2004 [MIT, Chemistry] Scott Tharp May 2003-May 2005 [Honors; U of IA, Biochemistry 99:155]

Thesis title: “A New Probe for Primary and Secondary Hydrogen Coupling in the Dihydrofolate Reductase Catalyzed Reaction”.

Majd Haddad May 2005-May 2006 [Honors; U of IA, Biochemistry 99:155] Thesis title: “The Study of Flavin Dependent Thymidylate Synthase”.

Pierre Charly Point Du Jour May 2005-Aug 2005 [SROP summer student form Florida Memorial U., FL, Biochemistry] Wilmarie Santana May 2005-Aug 2005 [SROP summer student form U. Puerto Rico- Bayamon, Biochemistry] Eric Koehn May 2006-Dec. 2007 [Honors; U of IA, Chemistry 4:192]

Thesis title: “Unraveling the Catalytic Mechanism of thyX- encoded Flavin Dependent Thymidylate Synthase”.

Hyunok (Cindy) Jo Aug 2006 – May 2008 [Honors; U of IA, Chemistry 4:192] Thesis title: “Analyzing the Variables Affecting Sensitivity of Primary Kinetic Isotope Effects in Dihydrofolate Reductase (DHFR).

Hyun Jo May 2008 – May 2009 [U of IA, Chemistry] Michael Schultz Aug. 2008 – Aug 2009 [U of IA, Chemistry] Daniel Willmer May 2009 – Aug 2009 [Coe College, IA, Chemistry] Esther Jean-Glles May 2009 – Aug 2009 [SROP/McNair summer student form Brooklyn College, NY, Chemistry and Biochemistry] Michael Toraason May 2009 – Dec. 2010 [U of IA, Chemistry] Asad Hashmi May 2009 – May 2010 [U of IA, Chemistry] Celvin Luzum May 2009 – May 2010 [U of IA, Chemistry]

Kohen, A.

43

Erika Torres May 2010 – July 2010 [SROP/McNair summer student form U of Porto Rico, Chemistry] Angela Torres May 2010 – July 2010 [SROP/McNair summer student form U of Porto Rico, Chemistry] Jordan Koehn May 2010 – Aug. 2010 [U of Minnesota, Biochemistry] Laura Tvedte May 2010 – July 2010 [Truman State U, MO] Emily Batkie Aug. 2010 – May 2011 [U of IA, Chemistry] Pablo Marrero June 2011 – July 2011 [SROP/McNair summer student form U of Porto Rico, Chemistry] Eva Serem June 2011 – July 2011 [SROP/McNair summer student form Minnesota State University at Mankato] Salah Moghram June 2011 – present [U of IA, Biochemistry]

John Corcoran Aug. 2011 – Aug. 2013 [U of IA, Chemistry] DoC Undergraduate Award/Scholarship for 2012

Lena Kytmanova Jan. 2012 – Aug. 2012 [U of IA, Chemistry]

Casey Callandre Eyman Summer 2012 [Grinnell College]

Mahmoud Elkhalifa Aug. 2012 – May 2014 [U of IA, Chemistry]

Christine Schwartz Aug. 2012 – Dec. 2012 [U of IA, Chemistry]

Kyle Wickersham Aug. 2012 – present [U of IA, Chemistry]

Daniel Watson Aug. 2012 - Aug. 2013 [U of IA, Chemistry]

Catherine Suchanek Aug. 2012 - present [U of IA, Chemistry] American Institute of Chemists award for 2014

Erica Drapeau Jan. 2013 – Aug. 2013 [U of IA, Chemistry]

Devin Sweet Summer 2013 (U of IA, Biochemistry]

Timothy Strutzenberg June 2013 – Present [U of IA, Biochemistry] Simms Scholarship for 2014

Hepeng Ye Jan. 2014 - present [U of IA, Chemistry]

Ariel Kleinschmidt June 2014 - present [U of IA, Chemistry]

Jonathan Krueger June 2014 - present [U of IA, Chemistry]

SERVICE Service at the University of Iowa

Departmental service * Departmental Library Committee (1999-2004) * ACS Student Affiliate Committee (1999-2002) * Temporary Advisor of new “Organic” Graduate Students (2000-2005 and 2007-2009) * Student Travel Award Committee (2005) * Faculty Search Committees (Organic 2005 and Physical 2007) * Review committee for Lecturers (Spring 2004 and 2007)

Kohen, A.

44

* Participated in New Faculty Search and Selection process (2000-2009) * Participated in Graduate Student Recruiting (2000-2009) * Participated in Working Weekends at Iowa (2000-2008) * Search committee for Lecturers (2006) * Delivered recruiting seminars at local colleges (about once a year 2000-2009) * Pre-tenured faculty review committees (Chair, 2006 and 2007) * Co-director of Graduate Studies (2006-2009) * Undergraduate Advisor for Chemistry Majors (2005 – 2010) * Director of Graduate Studies (2010 – present) * Chair of Graduate Review Curriculum Committee (2010 - Present) * Executive Committee (2010 - Present). * Post tenure review committee for Quinn (Chair, w/ Small, 2011) * Hosted departmental colloquium speakers (ca. once a year 1999-2012) * Salary Committee 2012 * Mishtu Dey’s Review Committee (Chair, w/ Quinn, 2012) * Mishtu Dey’s Review Committee (w/ Messerle, 2013) * Five-year post-tenure review for Lou Messerle (w/ MacGillivray, 2013) * Mishtu Dey’s Review Committee (Chair, w/ Haes, 2014) * Computer Facility (2013-present) * Faculty Mentor of Ned Bowden (2014 - present) * Faculty Mentor of Renee Cole (2014 - present)

CLAS service * CLAS Faculty Assembly, (2006 – 2009). * CLAS Faculty Assembly Nomination Committee (2008). * Serving as reviewer on the external review for the Linguistics Department (Fall 2011)

CGS service * Postdoctoral Scholars Advisory Committee, PSAC (2007-2008). * Presidential Graduate Fellows program reevaluation committee (2011). * James F. Jakobsen Graduate Conference, University of Iowa, Iowa City, IA; faculty judge of student papers

(2012).

Interdisciplinary and University service * Executive Committee of the Center for Biocatalysis and Bioprocessing (2002-present) * Biosciences Advisory Committee (2003-2008) * Admission Committee of the UI Biosciences Program (2000-2006) * Served on Comprehensive Exams and Thesis Committees in Chemistry (~50), Chem. Biochem. Eng. (2), and

Biochemistry (3). * Round-table facilitator, CIC Summer Research Opportunities Programs (SROP) Conference of the UI

Graduate College (2004) * MPSFP VPR Funding Review Committee (2006, 2011) * Biosciences Program Advisor (2005, 2006, 2008, 2009, 2010, 2011) * MSTP Faculty Member (2005- present; last renewal of this appointment May 2013 until July 2008) * EHS: Basic science radiation protection committee (2009 – present) * Honors Faculty at the Iowa Honors Program (2009 - present) * Presentation of research in the SROP summer program (2011) * Presidential Graduate Fellows program evaluation committee – College of Graduate Studies (2011) * Mentorship committee for Prof. Michael Schultz (a compulsory committee in Radiology and in Free Radical

Radiation Biology Program – 2011 - present)

Kohen, A.

45

* Serving as reviewer on the external review for the Department of Pharmacology, UI College of Medicine (Winter 2012-2013).

* Conducting a class visit and report for the Department of Radiology and Radiation Oncology for P&T of Mike Schultz (Sept. 2013).

* MSTP Admissions Committee [~200 applications annually] (2013 – present) * Member of the University Judicial Commission (2014 – 2017) * Director of the UI Training Program in Biotechnology (2013- present)

External Service -- National and International Conference Organization and Guest Editor

• Editorial Board of J. Biol. Chem. (2015-2020). • Co-Organizer (with Chris Cheatum and Robert Challender) “The Role of Dynamics in Enzyme

Catalyzed Reactions” in Telluride, CO, Aug. 3-7, 2015. • Scientific Committee member for the ISOTOPR 2015. • ASBMB Publications Committee (American Society for Biochemistry and Molecular Biology, publisher of

J. Biol. Chem., Mol. Cell. Proteomics, J. Lipid. Res., and more). 2014-2017. • Advisory Panel for Program Project from NIGMS (PIs: Vern Schramm, Steve Schwartz, Brian Dyer, and

Robert Challender), 2104 – 2018. • Chair, Gordon Research Conference on Isotopes in chemistry and Biology, Galveston, TX, Feb. 2-7, 2014. • Co-Organizer (with Chris Cheatum and Robert Challender) “The Role of Dynamics in Enzyme

Catalyzed Reactions” in Telluride, CO, July 29-Aug. 2, 2013. • Executive Committee and presenter of Closing Remarks, ISOTOPES 2013, Gdynia, Poland, June 16-21,

2013. • Vice Chair, Gordon Research Conference on Isotopes in chemistry and Biology, Galveston, TX, Feb. 5-10,

2012. • Scientific Committee, ISOTOPES 2011, Gréoux-les-Bains, France, June 20-24, 2011. • Organizing Committee, ISOTOPES 2009, Cluj-Napoca, Romania May 25-29, 2009. • ACS Symposium Co-Organizer with Prof. Arieh Warshel at the ACS National Meeting (Division of

Physical Chemistry), Symposium title: Functional Motions in Enzyme Catalysis. Salt Lake City, Utah, March 22-27, 2009.

• Guest Editor Proc. Natl. Acad. Sci. USA (2008). • Organizing Committee, 17th Annual Center for Biocatalysis and Bioprocessing Conference, Oct. 20-21,

2008 Iowa City, IA. • Chair “XXVIII Midwest Enzyme Chemistry Conference”, University of Chicago, IL, Oct. 5, 2008. • Organizing Committee “QAMTS 2007”, Huston, TX, Oct. 28-Nov. 1, 2007. • Executive Committee “Isotopes 2007”, Castellon, Spain, May 27-June 1, 2007. • Discussion Leader at the Gordon Research Conference on Isotope in Chemistry and Biology, Ventura, CA.

Feb. 17-22, 2006. • Program Committee of the International Conference on Quantum Atomic and Molecular Tunneling in

Solids (QAMTS), Santiago de Compostela, Spain, July 19-21, 2005. • Discussion Leader at the Gordon Research Conference on Enzymes, Cofactors, and Metabolic Pathways,

Kimball Union Academy, Meriden, NH. July 18-23, 2004.

Kohen, A.

46

• ACS Symposium Organizer with Prof. Arieh Warshel at the ACS National Meeting (Division of Physical Chemistry), Symposium title: Structure Function Correlation of Biomolecules: Enzyme Action. Orlando FL. April 7-11, 2002.

• Session Organizer and Chair at the Gordon Research Conference on Isotope Effect in Chemistry and Biology, Session title: Tunneling in Enzyme Catalysis, Feb. 17-24, 2002, Ventura, CA.

• Organizing Committee of the 10th Annual Center for Biocatalysis and Bioprocessing Conference, Oct. 22-24, 2001 Iowa City, IA.

Editorial Boards

• Journal of Biocatalysis & Biotransformation (2012 – present)

External Examiner and Evaluator • External Examiner on a thesis committee – Knox College, May 26, 2004 (B.Sc. Honors) • External Examiner on a thesis committee – Albert Einstein college of Medicine, Sep. 14, 2004 (Ph.D.). • Review of a Promotion and Tenure Package for the School of Natural Sciences, UC Merced (2010). • External Examiner on a thesis committee – Albert Einstein college of Medicine, Sep. 14, 2012 (Ph.D.). • Reviewing promotion to Full Professor – Indiana University – Purdue University Indianapolis, 2014. • Reviewing promotion to Full Professor – Case Western Reserve University, 2014.

Review for Professional Journals and Granting Agencies

Manuscript Review for Professional Journals and Other Publications:

• J. Am Chem. Soc. (68) • Biochemistry (43) • J. Phys. Chem. (25) • Proc. Natl. Acad. Sci. USA (13) • Nature (6) • Science (5) • Chem. Rev. (3) • Acc. Chem. Res. (2) • Nature Chemical Biology (1) • Nature Chemistry (1) • Nature Commun. (2) • Protein Science (1) • J. Phys. Org. Chem. (3) • J. Proteome Res. (1) • J. Biol. Inorg. Chem. (1) • Mol. Pharmaceutics (1) • Bioorg. Chem. (1) • Chem. Comm. (2) • J. Biol. Chem. (5) • Biochem. Biophys. Acta (3) • Archives of Biochem. Biophys. (5) • Anal. Biochem. (5) • Biotechnology Progress (1)

• Phil. Trans. R. Soc. B (2) • J. Theor. Biol. (2) • Book chapters (4) • Molecular Microbiol. (1) • J. Inorg. Biochem. (1) • J. Bacteriol. (3) • Biophys. J. (2) • J. Org. Chem. (1) • Z.f. Phys. Chemie (1) • Environmental Sci. Technol. (1) • ACS Chem. Biol. (1) • ChemPhysChem (1) • Israel J. Chem. (2) • FEBS J. (2) • Wiley-Blackwell Publishers (2) • Rapid Commun. Mass Spect. (1) • Phys. Chem. Chem. Phys. (1) • J. Agric. Food Chem. (1) • FEMS Microbiol. Lett. (1) • PLoS ONE (4) • Open Biol (1) • ChemBioChem (3) • J. Proteomics (2)

Kohen, A.

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• Molecules (2) • J. Bioterrorism & Biodefense (1)

• Biochem. J. (2) • J. Biomolecular Struct. Dyn. (1)

Grant Review Panels: • NIH study session, Ad Hoc Panelist, MSFE Feb. 2008. • NSF panel, CHE-MCB joint session Nov. 2008. • Royal Society of Chemistry, UK, Chair of Scientific Advisory Group for LOLA grant, Feb. 2009. • NIH Special Emphasis Panel ZRG1, March 2009. • NIH RC1, Challenge Grants, ZRG1 BCMB P 58 R, July 2009. • NIH study session, Ad Hoc Panelist, MSFE Oct. 2009. • NIH Special Emphasis Panel ‘Special Topics in Biophysics’ Study Section, Dec 19, 2011. • NIH MSFE study session: Permanent member and Co-Chair 2010-2014. • NSF panel, CHE-MCB joint session Nov. 2012. • Research Cooperation for Science Advancement, Aug 2013. • Chair of a Special Emphasis NIH panel ZRG1 BCMB, Feb. 2014.

Mail Reviewer for Funding Agencies: • NSF (38) • USDA (3) • ACS-PRF (9) • U.S. Civilian Research and Development Foundation (1) • BBSRC/ Research Councils UK (4) • BSF (USA-Israel) (4) • ICTS (8)

Outreach Activities

• Iowa State Fair, Aug. 13, 2009.

• Teaching two science classes in Roosevelt Elementary School (2009, 2010).

• Teaching six science classes in Northwest Junior High per spring (2010, 2011). • iExploreSTEM STEM in Iowa expo (2011).

• Judge at The Eastern Iowa Science & Engineering Fair (EISEF http://www.eisef.org), March 17, 2011 and March 15, 2014.

• Advising summer teacher in Belin-Blank National Scholars Institute session entitled “Emzymes in life and medicine”, Summer 2014.

• Hosting students as part of the Solon Middle School STEM Tours (Workplace Learning Connection) 2014.

• United Teach volunteer (2014)