Key Enabling Technologies for Point-of-Care Diagnostic

SensingWout Knoben - Surfix

Aliki Tsopela – Micronit Microtechnologies

About Surfix

Our conviction

Micro- and nanotechnology are an integralpart of sustainable solutions for majorglobal challenges in healthcare, food andenergy.

At Surfix, we believe that accurate andprecise control of surface properties willenable our customers to exploit the fullpotential of their world changing devices.

About Surfix

A nanocoating for everyone• Tailor-made nanocoatings

− substrate material− surface properties− coating architecture− patterning

• Services− surface characterization− coating of devices− technology transfer− close collaboration

1-2 nm

10-100 nm

3D nanocoating

2D nanocoating

Surfix technology

Nanocoating options• Chemical surface modification

• Covalent attachment of organic molecules

• Coating architectures− 2D− 3D

• Patterning options− uniform− local− material-selective

material-selective nanocoating

local nanocoating

Surfix technology

Nanocoating options• Chemical surface modification

• Covalent attachment of organic molecules

• Coating architectures− 2D− 3D

• Patterning options− uniform− local− material-selective

Micronit microtechnologies – key facts

6

ISO9001

ISO13485

EnschedeThe Netherlands

Dortmund Germany

1000+we served

customers

50+ countries

in

100+ highlyeducated

multi-disciplinarypeople

founded in

Micronit microtechnologies - Our core business

Quality manufacturing

• Low-High-volume manufacturing capabilities

Creative product development

• Co-engineering

• Functional microfluidics design

• Design for manufacturing

Research products & prototyping services

• Off-the-shelf standard microfluidic chip modules

• Custom prototyping & low-volume manufacturing

7

Any material or combination of materials

8

HYBRID

HYBRID

HYBRID

SILICON POLYMERS

GLASS

Source: Yole Développement

Point-of-Care (PoC) devices

Medical tests at the point of care Market analysis

Coat PoCKET

Local surface modificationElectrostatically actuated capillary valves

Development of a capillary flow based immunoassay chip

Point-of-Care (PoC) diagnostics at Micronit

Antibody immobilization

Surfaces passivation

Antigen capture

Detection antibody

Labeling

Detection

Washing

ELISA on chip for the detection of cardiac biomarkers

Point-of-Care (PoC) diagnostics at Micronit

ELISA on chip for the detection of cardiac biomarkers

Integrated capillary flow control

Capillary burst valves

• Hot embossing and micromilling of polymers

• Increase in the depth and width of the channel just after the valve

20μm

20μm

Electrostatic actuation mechanism

• Thin film metal patterning on polymers

• Application of voltage between two electrodes for meniscus triggering

14

Integrated capillary flow control

Integrated capillary flow control

Sequential flow chip

• Multistep assays

• Sequential loading of liquid solutions

activated COC (bonding)

hydrophilic channel

hydrophobic patch

Coat PoCKET

Coating challenges• patterned coating of COC

• two patterning steps on one substrate

• alignment of coating to− channels− electrodes− previous coating

• compatibility with bonding− surface activation− temperature− solvent

Patterned coatings on ‘flat’ COC

Single patterning• WCA untreated COC: 95o

• WCA activated COC: 60o (temporary)

• WCA hydrophilic coating: 45o

• WCA hydrophobic coating: 115o

Double patterning• best result obtained

− hydrophobic patch on top of hydrophilic coating− WCA 45o vs. 125o (!)− coatings are compatible with thermal bonding conditions

m

Patterned coatings in COC channels

Coating of COC channels• alignment of coating with channels/electrodes

• coating in ‘deep’ channels

• hydrophilic coating works well

• hydrophobic coating more difficult to align

Bonding• is the uncoated COC surface still bondable?

• thermal fusion bonding (120 oC, 20 min)

• bonding works well if done on same day as coating400 mm

Status and outlook

Status• double patterned coating and bonding of COC devices is possible

• encouraging results from first batch of test devices (proof of principle)

• second batch should yield full functionality

Outlook• improve alignment of hydrophobic coating

• development of demonstrator assay

• development of integrated wafer level fabrication process

• Coat PoCKET project runs until the end of 2018

Concluding remarks

Summary• Capillary flow control and (patterned) surface modification are key

enabling technologies for the next generation PoC diagnostic devices

• Micronit Microtechnologies and Surfix are developing a demonstrator assay to show the potential of the combination of these technologies

More information• Micronit Microtechnologies

− booth− presentation at MicroNanoConference: 13:30 (Veilingzaal)

• Surfix− booth− presentation at MicroNanoConference: 14:00 (Veilingzaal)