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12/14/2015 Kenneth Vincent Mills
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Kenneth Vincent Mills College of the Holy Cross Phone: 15087933380Department of Chemistry FAX: 15087933530 1 College Street Email: [email protected], MA 01610 Employment2001present College of the Holy Cross, Department of Chemistry, Worcester, MA
Professor, 2014Department Chair, 2010 2014Associate Professor, 20072014Assistant Professor, 20012007
Education19962001 Harvard University, Cambridge, MA
Department of Chemistry and Chemical BiologyA.M. 1998, Ph.D. 2001, Advisor: Prof. Henry Paulus
19921996 Loyola College in Maryland, Baltimore, MD B.S., summa cum laude, 1996, Chemistry major, philosophy minor Research InterestsProtein splicing, mechanistic enzymology, protein chemistry, guidedinquiry undergraduate lab development Research Experience2001present College of the Holy Cross, Department of Chemistry, Worcester, MA Direct undergraduate researchers in the study of the mechanism of hedgehog autoprocessing and protein splicing. 19962001 Harvard University, Department of Chemistry and Chemical Biology, Cambridge, MA
Studied mechanism of protein splicing and developed an in vitro transsplicing protein ligation system using inteins fromRecA protein of Mycobacterium tuberculosis and DnaE protein of Synechocystis species PCC6803.
1995 Summer Research Institute for Applied Science, Lawrence Livermore National Laboratory, Livermore, CA
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Studied peptide models of prion protein folding. 19911996 Medical Research Institute for Chemical Defense, Aberdeen Proving Grounds, Edgewood Area, MD
Studied and designed active site mutants of human butyrylcholinesterase that demonstrated hydrolysis of organophosphateinhibitors.
Teaching Experience2001present College of the Holy Cross, Department of Chemistry MontserratDrug Design, Action and Abuse (Natural World cluster, Fall 2012 and 2013) MontserratBiotechnology and Ethics (Natural World cluster, Spring 2013 and 2014)
Atoms and Molecules, Chemistry 101/181 Lecture and Lab (Fall 2001, 2002, 2004, 2005, 2006, 2008)Introduction to Equilibrium and Reactivity/Equilibrium and Reactivity, Chemistry 231 Lecture and Lab (Spring 20022007,200912)Biochemistry, Chemistry 301, Fall 2008, 201013Bioorganic Chemistry, Chemistry 310 (Spring 2003, 2006)Advanced Biochemistry, Chemistry 363 (Spring 2004)Biochemistry II Laboratory, Biology 304 (Spring 2002)
20002001 Harvard University, Division of Continuing Education Head teaching fellow for General Chemistry course. 19972000 Harvard University, Division of Continuing Education
Teaching fellow for General Chemistry
19961997 Harvard University, Department of Chemistry and Chemical BiologyTeaching fellow for Organic Chemistry
External Grants 20152018 National Science Foundation, RUI Grant, $748,268 RUI: The Catalysis of Protein Splicing and the Structure and Stability of Thermophilic Inteins: An Integrated Researchand Education Program 20132016 National Science Foundation, RUI Grant, $567,115 RUI: Intein Structure and Catalysis of Protein Splicing and Hedgehog Autoprocessing: An Integrated Research and EducationProgram
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2012 National Science Foundation, REU supplement to RUI grant, $6,500 20102013 National Science Foundation, RUI Grant, $465,000 RUI: Catalysis and Coordination of the Steps of Protein Splicing by Noncanonical Inteins: An Integrated Research andEducation Program 20092016 Henry Dreyfus Teacher Scholar Award, $60,000 2010 National Institutes of Health, Subaward, $25,000 Subaward to collaborator Chunyu Wang’s NIH ARRA supplement (Rensselaer Polytechnic Institute) 20052010 National Science Foundation, CAREER grant, $794,482
“CAREER: Alternative Mechanisms of HINT domain autoprocessing: An integrated undergraduate research and educationprogram”
2009 American Society for Biochemistry and Molecular Biology, Undergraduate Outreach Award, $250 20032005 National Science Foundation, Major Research Instrumentation, $255,502
“Acquisition of Protein Purification and Analysis Equipment to Enhance Biochemical Research and Education at the Collegeof the Holy Cross”
20032005 Research Corporation, Cottrell College Science Award, $58,028
“The Mechanism and Molecular Evolution of Hedgehog Proteins and Related AutoProcessing Domains” 20022004 Petroleum Research Fund (American Chemical Society), $35,000 Type GB, “The Mechanism of Protein Splicing and the Molecular Evolution of Inteins.” 19972001 Howard Hughes Medical Institute Predoctoral Fellowship 19951996 Barry M. Goldwater Fellowship Internal Grants 2013 Hewlett Mellon Presidential Discretionary Fund Summer Workshop on Emerging Challenges in STEM Education 2012 Hewlett Mellon Presidential Discretionary Fund, Chemistry
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Chemistry Department Review: Reflection and Response 2009 Hewlett Mellon Presidential Discretionary Fund, Biochemistry Concentration Assessment: Refinement and Implementation 2008 Hewlett Mellon Presidential Discretionary Fund, Biochemistry Concentration Assessment: Mission, Goals and Student
Learning Outcomes 2003 Research and Publication Award 2002 Charles and Rosanna Batchelor (Ford) Foundation Summer Fellowship (declined to accept external funding) Awards/Fellowships 2015 Cottrell Scholar, Research Corporation2013 Mary Louise Marfuggi Award – Outstanding Scholarly Accomplishments2009 Henry Dreyfus Teacher Scholar Award, Dreyfus Foundation2009 Undergraduate Outreach Award, American Society for Biochemistry and Molecular Biology2002 Charles and Rosanna Batchelor (Ford) Foundation Faculty Fellowship19972001 Howard Hughes Medical Institute Predoctoral Fellowship1996, 1997 National Science Foundation Fellowship honorable mention1996 American Institute of Chemists, D.C. chapter, chemistry award19941996 Barry M. Goldwater Scholar19941996 Datatel Scholar1996 Award for highest overall GPA, Loyola College in Maryland1996 Chemistry Medal, Loyola College in Maryland1995 Phi Beta Kappa, Alpha Sigma Nu (Jesuit Honor Society)19921996 Dean's List, Loyola College in Maryland, all semesters19921996 Marion Burk Knott Scholarship, Maryland Distinguished Scholar19921993 National Merit Scholar, Byrd Scholar Publications (Undergraduate coauthors indicated by asterisk) Holy Cross publications: 30. Mills, K.V. (2015) Biochemistry in an undergraduate writingintensive firstyear program: Seminar courses in drugs and bioethics.Biochemistry and Molecular Biology Education 43, 263272.
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29. Kulevich, S.E., Herrick, R.S., and Mills, K.V. (2014) A Discovery Chemistry Experiment on Buffers. J. Chem. Educ. 91, 12071211. 28. Mills, K.V., Johnson, M.A., and Perler, F.B. (2014) Protein splicing: How inteins escape from precursor proteins. Journal of BiologicalChemistry, 289, 1449814505. 27. Topilina, N.I. and Mills, K.V. (2014) Recent Advances in in vivo applications of inteinmediated protein splicing. Mobile DNA 5, 5. 26. *Nicastri, M.C., *Xega, K., Li, L., Xie, J., Wang, C., Linhardt, R.J., Reitter, J.N., and Mills, K.V. (2013) Internal Disulfide Bond Acts asa Switch for Intein Activity. Biochemistry 52, 59205927. 25. Mills, K.V. (2013) SelfSplicing Proteins. In: Handbook of Proteolytic Enzymes, Third Edition, Vol. 1., ed. N. Rawlings and G. Salvesen,315321. 24. Chen, W., Li, L., Du, Z., Liu, J., Reitter, J.N., Mills, K.V., Linhardt, R.J. and Wang, C. (2012) Intramolecular Disulfide Bond betweenCatalytic Cysteines in an Intein Precursor. J. Am. Chem. Soc.,134, 25002503. 23. Du, Z., Liu, J., Albracht, C.D., Hsu, A., Chen, W., *Marieni, M.D., *Colelli, K.M., *Williams, J.E., Reitter, J.N., Mills, K.V. and Wang,C. (2011) Structural and Mutational Studies of a Hyperthermophilic Intein from DNA Polymerase II of Pyrococcus abyssi. J. Biol. Chem.286, 3863838648. (Selected for Nov. 4, 2011 cover.) 22. Liu, J., Du, Z., Albracht, C.D., Naidu, R.O., Mills, K.V. and Wang, C. (2011) 1H, 13C, and 15N NMR assignments of the Pyrococcusabyssi DNA polymerase II intein. Biomol. NMR Assign. 5, 233235. 21. Reitter, J.N. and Mills, K.V. (2011) Canonical protein splicing of a class one intein that has a class three noncanonical sequence motif.J. Bacteriol. 194, 994997. 20. *O’Brien, K.M., *Schufreider, A.K., *McGill, M.A., *O’Brien, K.M., Reitter, J.N. and Mills, K.V. (2010) Mechanism of proteinsplicing of the Pyrococcus abyssi lon protease intein. Biochem. Biophys. Res. Commun. 403, 457461. 19. *Kerrigan, A.M., *Powers, T.L., *Dorval, D.M., Reitter, J.N., and Mills, K.V. (2009) Protein splicing of the three Pyrococcus abyssiribonucleotide reductase inteins. Biochem. Biophys. Res. Commun. 387, 153157. 18. Mills, K.V., Herrick, R.S., Ditzler, M.A., Nestor, L.P., Shafer, H., and Guilmette, L.W. (2008) An Introduction to Electrochemistry: ATwoweek Discovery Chemistry Experiment. J. Chem. Educ. 85, 11161119. 17. Herrick, R.S., Mills, K.V., and Nestor, L.P. (2008) Data Pooling in a Chemical Kinetics Experiment: The Aquation of a Series of Cobalt(III) Complexes. J. Chem. Educ. 85, 11201122.
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16. Mills, K.V. and Guilmette, L.W. (2007) Thermochemical Analysis of Neutralization Reactions: An Introductory Discovery Experiment.J. Chem. Educ. 84, 326328. 15. Bellin, R.M. and Mills, K.V. (2007) The Holy Cross Biochemistry Concentration: An Integrated FourYear Program to DevelopUndergraduate Research Scholars. In Developing and Sustaining a ResearchSupportive Curriculum, ed K.K. Karukstis and T.E. Elgren,Council on Undergraduate Research. 14. Mills, K.V., Connor, K.R., *Dorval, D.M., and *Lewandowski, K.T. (2006) Protein Purification via TemperatureDependent, InteinMediated Cleavage from an Immobilized Metal Affinity Resin. Anal. Biochem. 356, 8693. 13. Mills, K.V., *Dorval, D.M., *Lewandowski, K.T. (2005) Kinetic analysis of the individual steps of protein splicing for the Pyrococcusabyssi PolII intein. J. Biol. Chem 280, 27142720. 12. Mills, K.V., *Gareau, J.R., and *Garcia, A.M. (2005) Lowcost modification to the Copacabana method for spreading transformationmixtures. BioTechniques 39, 188. 11. Mills, K.V., Perler, F.B. (2005) The Mechanism of InteinBased Protein Splicing: Variations on a Theme. Protein & Peptide Letters, 12,751755. 10. Mills, K.V. (2005) Protein Splicing. In Encyclopedia of Molecular Cell Biology and Molecular Medicine, Second Edition, ed, RAMeyers, 241265, Weinheim: WileyVCH. 9. Mills, K.V., Paulus, H. (2005) Biochemical Mechanisms of InteinMediated Protein Splicing. In Nucleic Acids and Molecular Biology 16(Homing Endonucleases and Inteins), ed. M. Belfort, 231255, Berlin: Springer Verlag. 8. Mills, K.V., *Manning, J.S., *Garcia, A.M., *Wuerdeman, L.A. (2004) Protein splicing of a Pyrococcus abyssi intein with a Cterminalglutamine. J. Biol. Chem. 279, 2068520691. 7. Mills, K.V. (2004) SelfSplicing Proteins. In: Handbook of Proteolytic Enzymes, Second Edition, Vol. 2., ed. AJ Barrett, 21362140,London: Academic Press, Elsevier Science. 6. Mills, KV. (2003) PostTranslational Control. In: Genetics, Vol. 3., ed. R Robinson, 178182, New York: Macmillan Reference USA. PreHoly Cross Publications: 5. Mills, K.V., Paulus, H. (2001) Reversible Inhibition of Protein Splicing by Zinc Ion. J. Biol. Chem. 276, 1083210838.
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4. Lew, B.M., Mills, K.V., Paulus, H. (1999) Characteristics of Protein Splicing in trans Mediated by a Semisynthetic Split Intein.Biopolymers (Peptide Science) 51, 355362. 3. Lew, B.M., Mills, K.V., Paulus, H. (1998) Protein Splicing in Vitro with a Semisynthetic TwoComponent Minimal Intein. J. Biol.Chem.273, 1588715890. 2. Mills, K.V., Lew, B.M., Jiang, Sq, Paulus, H. (1998) Protein splicing in trans by purified N and C terminal fragments of theMycobacterium tuberculosis RecA intein. Proceedings of the National Academy of Sciences, USA 95, 35433548. 1. Broomfield, C.A., Mills, K.V., Meier, B.M., Lockridge, O.A., and Millard, C.B. (1998) Reaction of human butyrylcholinesterase (BchE)H117 enzymes with carbamates. In: Structure and Function of Cholinesterases and Related Proteins, 223226. Abstracts (Recent, first author presenter) Jaramillo, M., Williams, J., Reitter, J. and Mills, K. (2015) Structure and Activity of Inteins from Pyrococcus abyssi and Pyrococcushorikoshii. FASEB J. 29, 722.4. Yakely, A., Bonano, A., Jaramillo, M., Williams, J., Reitter, J., and Mills, K. (2015) Relating Intein Flexibility to the TemperatureDependence of Activity. FASEB J. 29, 722.3. Siegart, N., Colelli, K., Reitter, J. and Mills, K. (2015) Protein Splicing of a TemperatureDependent Intein from an Extreme Thermophile.FASEB J. 29, 573.12.(Best Undergraduate Poster – Proteins and Enzymes: www.asbmb.org/News.aspx?id=63127) Giaccone, Z., Reitter, J., Steeves, A., Kulik, H. and Mills, K. (2015) Peptide Bond Cleavage through Asparagine Cyclization. FASEB J. 29,722.9. Koulopoulos, M., Giaccone, Z., Reitter, J., and Mills, K. (2015) A Biophysical Analaysis of Asp Cyclization Coupled to Peptide BondCleavage. FASEB J. 29, 722.2 Mills, K. (2015) Integrating Biochemistry into a Firstyear Undergraduate RhetoricIntensive Seminar Program. FASEB J. 29, 559.1. Mills, K., Reitter, J., Nicastri, M., Colleli, K., and Williams, J. (2015) Protein splicing: Regulation by Temperature and Oxidation State ofNonCanonical Inteins, Biophysical Journal 108 (2Sup.1), 30a 144Plat. *Jaramillo, M.V., *Nicastri, M.C., Reitter, J.N. and Mills, K.V. (2014) Protein splicing of an intein from the extreme halophileHalobacterium salinarum. FASEB J. 28, 768.9.
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*Haley, D.S., *Siegart, N.M., Reitter, J.N. and Mills, K.V. (2014) Structural analysis of human carbonic anhydrase II and a thermophilicintein. FASEB J. 28, 768.3. *Cahn, S.A.,Reitter, J.N. and Mills, K.V. (2014) Directed evolution toward inteins with alternative protein splicing mechanisms. FASEB J.28, 566.2. *Giaccone, Z.T., *Urbanski, L.M., *Siegart, N.M., Reitter, J.N. and Mills, K.V. (2014) Peptide bond cleavage through cyclization ofasparagine. FASEB J. 28, 768.4. *Siegart, N.M., *Colelli, K.M., *Williams, J.E., Reitter, J.N. and Mills, K.V. (2014) Protein splicing of a temperaturedependent intein froman extreme thermophile. FASEB J. 28, 768.10. Mills, K.V., Reitter, J.N., *Nicastri, M.C., *Williams, J.E., and *Colelli, K.M (2014) The stops and starts of protein splicing: regulation andmechanism of noncanonical inteins. FASEB J. 28, 584.1. *Siegart, N.M., *Urbanski, L.M., *Karanja, K., Reitter, J.N. and Mills, K.V. (2013) Protein Splicing of Noncanonical Inteins fromCyanobacteria Protein Science 22Sup1, 234. Mills, Kenneth V., Reitter, J.N., *Nicastri, M.C., *Williams, J.E., *Colelli, K.M., and *Marieni, M.D. (2013) The Structure and Regulationof NonCanonical Inteins. Protein Science 22Sup1, 93. *Williams, J.E., Reitter, J.N. and Mills, K.V. (2013) Structural analysis of an intein from an extreme thermophile. FASEB J. 27, 1005.1.(Honorable Mention – Best Undergraduate Poster) *Colelli, K.M., *Pusztay, J.M., Reitter, J.N. and Mills, K.V. (2013) The role of an extended betasheet in stabilizing the structure of athermophilic intein. FASEB J. 27, 1005.2. (Honorable Mention – Best Undergraduate Poster) *Nicastri, M.C., *Xega, K., Reitter, J.N., and Mills, K.V. (2013) Conditional protein splicing via disulfide bond formation. FASEB J. 27,789.3. *Siegart, N.M., *Urbanski, L.M., *Karanja, K., *Colelli, K.M., Reitter, J.N. and Mills, K.V. (2013) Protein splicing of inteins fromSynechococcus sp. PCC7002 and Trichodesmium erythraeum. FASEB J., 27, 789.7. *Cahn, S.A., *Savidis, G., *Drago, M.J., Reitter, J.N. and Mills, K.V. (2013) Expression and autoprocessing of hedgehoglike proteins fromBrugia malayi and Cryptosporidium. FASEB J. 27, 789.4. *Pusztay, J.M., *Drago, M.J., *Powers, T.L., *Schufreider, A.K., Connor, K.R., Reitter, J.N., and Mills, K.V. (2013) Splicing of a non
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canonical class three intein from Clostridium thermocellum. FASEB J. 27, 789.5. *Urbanski, L.M., *Chin, S.L., Reitter, J.N. and Mills, K.V. (2013) Peptide bond cleavage adjacent to asparagine or glutamine. FASEB J. 27,789.6. Mills, K.V., Reitter, J.N., Connor, K.R., *Nicastri, M.C., *Williams, J.E., *Colelli, K.M., and *Marieni, M.D. (2013) The structure,regulation and activity of noncanonical inteins. FASEB J. 27, 998.2. *Savidis, G., *Drago, M.J., Reitter, J.N., Mills, K.V. (2012) Expression and autoprocessing of hedgehoglike domains from Brugia malayi.FASEB J. 26, 753.2. *Colelli, K.M., Reitter, J.N. and Mills, K.V. (2012) Protein splicing of a temperaturedependent intein from an extreme thermophile. FASEBJ. 26, 756.3. (Honorable Mention – Best Undergraduate Poster) *Williams, J.E., Reitter, J.N. and Mills, K.V. (2012) Protein splicing facilitated by highly similar inteins from two extreme thermophiles.FASEB J. 26, 756.4. *Karanja, K.K., *Urbanski, L.M., Reitter, J.N. and Mills, K.V. (2012) Protein splicing of inteins from Synechococcus sp. PCC 7002 andPyrococcus abyssi. FASEB J. 26, 756.5. *Urbanski, L.M., *Chin, S.L., Reitter, J.N. and Mills, K.V. (2012) Sidechain cyclization of Gln or Asn coupled to peptide bond cleavage inan intein and a model peptide. FASEB J. 26, 756.6. *Chin, S.L., *Nadelson, A.C., *Urbanski, L.M., Reitter, J.N. and Mills, K.V. (2012) Inteinmediated peptide bond cleavage adjacent toasparagine or glutamine. FASEB J. 26, 963.6. Mills, K.V., Reitter, J.N., Connor, K.R., *Nicastri, M.C., *Urbanski, L.M., *Williams, J.E., *Colelli, K.M., *Savidis, G. and *Marieni, M.D.(2012) Noncanonical inteins: Protein splicing by alternate mechanisms. FASEB J. 26, 959.2. *Nicastri, M.C., *Xega, K., Reitter, J.N. and Mills, K.V. (2012) Conditional protein splicing of inteins with a noncanonical Cterminalglutamine. FASEB J. 26, 959.1. Mills, K., Reitter, J., *Dorval, D., *Lewandowski, K., *Marieni, M., *Duffee, L., *Donahue, C. (2011) Noncanonical inteins: Alternatemechanisms for protein splicing. Protein Science 20Sup 1, 180. *Xega, K., Reitter, J.N. and Mills, K.V. (2011) Posttranslational autoprocessing by an Oryza sativa hedgehoglike domain. FASEB J. 25,754.16.
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*O’Brien, K.M., *Schufreider, A.K., *McGill, M.A., *O’Brien, K.M., Reitter, J.N. and Mills, K.V. (2011) Posttranslational autoprocessingof a Pyrococcus abyssi intein and a Cryptosporidium hedgehoglike domain. FASEB J. 25, 754.10. *Marieni, M.D., *Duffee, L.R., *York, D.J., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2011) The influence of conserved catalyticresidues on the mechanism of protein splicing of the Pyrococcus abyssi PolII intein. FASEB J. 25, 520.10. *Donahue, C.A., *York, D.J., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2011) Determining the activation barrier and pHdependence ofeach step of protein splicing. FASEB J. 25, 520.6. *Chin, S.L., *Nadelson, A.C., *York, D.J., Reitter, J.N. and Mills, K.V. (2011) Utilizing a protein splicing side reaction to generate afragment for expressed protein ligation. FASEB J. 25, 520.3. Mills, K.V., *Powers, T.L., *Drago, M.J., Connor, K.R., and Reitter, J.N. (2011) Noncanonical inteins: Alternate mechanisms for proteinsplicing. FASEB J. 25, 520.11. *Duffee, L.R., *York, D.J., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2010) A kinetic analysis of each step of protein splicing of thePyrococcus abyssi PolII intein. FASEB J. 24, 463.5. Mills, K.V., Reitter, J.N., *Duffee, L.R., *York, D.J., *Dorval, D.M., and *Lewandowski, K.T. (2010) Noncanonical inteins: alternatemechanisms for protein splicing. FASEB J. 24, 520.11. *Nadelson, A.C., *York, D.J., *Dorval, D.M., *Lewandowski, K.T., Connor, K.R., Reitter, J.N. and Mills, K.V. (2010) Manipulation ofprotein splicing side reactions to facilitate protein purification and expressed protein ligation. FASEB J. 24, 463.13. *York, D.J., *Duffee, L.R., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2010) Estimating the activation barrier to each step of proteinsplicing for the noncanonical P. abyssi PolII intein. FASEB J. 24, 463.18. (Honorable Mention – Best Undergraduate Poster) *Duffee, L.R., *Nadelson, A.C., *York, D.J., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2009) Influence of conserved residues onindividual steps of protein splicing of the noncanonical Pyrococcus abyssi PolII intein. Protein Science 18Sup1, 86. Selected for an oralpresentation. *Nadelson, A.C., *York, D.J., *Lewandowski, K.T., *Dorval, D.M., Connor, K.R., Reitter, J.N. and Mills, K.V. (2009) Development ofprotein purification strategies using the Pyrococcus abyssi PolII intein. Protein Science 18Sup1, 86. Mills, K.V., Reitter, J.N., Connor, K.R., *Kerrigan, A.M., *Dorval, D.M., *Drago, M.J.,*Lewandowski, K.T. and *Powers, T.L. (2009) Noncanonical inteins: Alternate mechanisms of protein splicing. Protein Science 18Sup1,171.
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*Schufreider, A.K., *McGill, M.M., *O’Brien, K.M., Reitter, J.N, and Mills, K.V. (2009) Protein Splicing of the Pyrococcus abyssi lonprotease intein. FASEB J. 22, 502.2. (Honorable Mention – Best Undergraduate Poster) *Kerrigan, A.M., *Powers, T.L., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2009) The influence of extein residues on the protein splicingof three Pyroccocus abyssi inteins. FASEB J. 22, 502.3. (Honorable Mention – Best Undergraduate Poster) *York, D.J., Nadelson, A.C., Reitter, J.N., and Mills, K.V. (2009) Alternate protein splicing mechanisms: A directed evolution approach.FASEB J. 22, 502.2. Reitter, J.N., *Schufreider, A.K., and Mills, K.V. (2009) Protein Splicing by a Noncanonical Mechanism. FASEB J. 22, 677.3. *McGill, M.A., *O’Brien, K.M., and Mills, K.V. (2008) The P. abyssi lonb protease: Protein splicing activity and proteolysis by solublefragments. Abstracts of the ASBMB 2008 National Meeting. *Kerrigan, A.M., *Powers, T.L., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2008) The dependence of three P. abyssi inteins on exteinsequence for efficient protein splicing. FASEB J. 20, 611.13. Mills, K.V., Reitter, J.N., Connor, K.R., *Dorval, D.M., *Drago, M.J., *Lewandowski, K.T., and *Powers, T.L. (2008) Alternatemechanisms of protein splicing by noncanonical inteins. Abstracts of the ASBMB 2008 National Meeting. Mills, K.V., Reitter, J.N., Connor, K.R., *Powers, T.L. and *Drago, M.J. (2007) Protein splicing of a noncanonical intein from C.thermocellum. Abstracts of Papers, 234th ACS National Meeting, Boston, MA, BIOL237. *McGill, M.A., *O’Brien, K.M., and Mills, K.V. (2007) Protein splicing and extein activity of the Pyrococcus abyssi lon protease intein. Protein Science 16Sup 1, 162. Mills, K.V., Reitter, J.N., Connor, K.R., *Powers, T.L., *Drago, M.J., *Lewandowski, K.T., and *Dorval, D.M. (2007) Alternative proteinsplicing mechanisms of inteins from C. thermocellum and P. abyssi. Protein Science 16Sup 1, 158. *Dorval, D.M., Connor, K.R., and Mills, K.V. (2006) Catalysis of individual steps of protein splicing of the Pyrococcus abyssi PolII intein. FASEB J. 20, A40. *Powers, T.L., *Drago, M.J., *Dorval, D.M., Connor, K.R., and Mills, K.V. (2006) Protein splicing of a noncanonical Clostridiumthermocellum intein with Nterminal Gln. FASEB J. 20, A964. Mills, K.V., *Lewandowski, K.T., and *Dorval, D.M. (2005) The Mechanism of Protein Splicing of the Pyrococcus abyssi PolII intein. Protein Science 14Sup 1, 87.
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*Lewandowski, K.T., *Dorval, D.M., and Mills, K.V. (2005) A Kinetic study of the protein splicing mechanism of the Pyrococcus abyssiPolII intein. FASEB J. 19, A1401. Mills, K., *Manning, J., *Garcia, A., *Wuerdeman, L. (2004) In vitro protein splicing of an intein with a Cterminal glutamine fromPyrococcus abyssi. FASEB J. 18, C276. *Lewandowski, K., *Wuerdeman, L., *Twomey, P., and Mills, K. (2004) Murine Sonic Hedgehog proteins: Subcloning, overexpression inE. coli and affinity purification. FASEB J. 18, C277. Invited Research Seminars Loyola University, Department of Chemistry, Baltimore, MD, February 10, 2015 Biophysical Society 59th Annual Meeting, Baltimore, MD, February 8, 2015 FASEB/ASBMB National Meeting 2014, San Diego, CA, April 27, 2014 Union College, Department of Chemistry, Schenectady, NY, October 11, 2012 Wellesley College, Biochemistry, Wellesley, MA, September 19, 2012 FASEB/ASBMB National Meeting 2010, Anaheim, CA, April 28, 2010 Pomona College/Harvey Mudd College, Claremont, CA, April 27, 2010 University of Massachusetts, Department of Chemistry, Amherst, MA, March 4, 2010 University of Vermont, School of Medicine, Burlington, VT, April 17, 2009 Trinity College, Hartford, CT, May 30, 2008 Worcester Polytechnic Institute, Worcester, MA October 12, 2005 Clark University, Worcester, MA April 1, 2002 New England Biolabs, Beverly, MA October 11, 2001 Gordon Research Conference, Biological Regulation Mechanisms
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Holderness, NH, July 30August 4, 2000 Other Selected Professional Presentations, Poster Presentations FASEB/ASBMB 2015, Boston, MA, March 29, 2015 Winter Enzyme Mechanism Conference, Galveston, TX, January 5, 2015 28th Symposium of the Protein Society, San Diego, CA, July 29, 2014 FASEB/ASBMB 2014, San Diego, CA, April 26, 2014 27th Symposium of the Protein Society, Boston, MA July 20, 2013 FASEB/ASBMB 2013, Boston, MA, April 23, 2013 25th Anniversary Symposium of the Protein Society, Boston, MA July 26, 2011 FASEB/ASBMB 2011, Washington, DC, April 10, 2011 Dreyfus Foundation, New York, NY, October 30, 2010 FASEB/ASBMB 2010, Anaheim, CA, April 25, 2010 23rd Symposium of the Protein Society, Boston, MA, July 2529, 2009 Gordon Research Conference, Enzymes, Coenzymes and Metabolic Pathways University of New England, Biddeford, ME, July 2008 IUBMB/ASBMB 2008, San Diego, CA April 8, 2008 The 234th American Chemical Society National Meeting, Boston, MA, August 21, 2007 TwentyFirst Symposium of the Protein Society, Boston, MA July 21July 25, 2007 Nineteenth Symposium of the Protein Society, Boston, MA July 30August 3, 2005
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IUBMB/ASBMB 2004, Boston, MA June 15, 2004 Gordon Research Conference, Biological Regulation MechanismsHolderness, NH, July 30August 4, 2000
Thirteenth Symposium of the Protein Society, Boston, MA, July 2428, 1999 Student Research Mentoring Summer Research Students Summer 2015Alexander Bonano (Chemistry ’17)Christopher Cousin (Chemistry ’16)Joshua Long (Biology ’17)Christopher Minteer (Chemistry/Biochemistry ’17)Isabelle Pierre (UMassDartmouth, ’15)Christian Ramsoomair (Chemistry ’18) Summer 2014Alexander Bonano (Chemistry ’17, NSF)Dawilmer Castillo (’15, Bridgewater State NSF)Michael Koulopoulos (Chemistry/Biochemistry ’16, Holy Cross)Zachary Giaccone (Chemistry/Biochemistry ’15, NSF)Mario Jaramillo (Biology ’15, NSF)Anthony Yakely (Chemistry/Biochemistry ’16, Dreyfus) Summer 2013Julliard del Rosario, ’16 Cornell University (NSF)Zachary Giaccone (Chemistry/Biochemistry ’15)Daniel Haley (Chemistry ‘14)Mario Jaramillo (Biology ’15)Nicolle Siegart (Biology/Biochemistry ’15) Summer 2012Sarah Cahn (Chemistry ’14, Becton Dickinson)Kathryn Colelli (Biology/Biochemistry ’13, Beckman Foundation)
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Nick Gulbrandsen (Chemistry ’13, Fitchburg State)Jennifer Pusztay (Chemistry/Biochemistry ’13, NSF)Nicolle Siegart (Biology ’15, NSF) Summer 2011Kathryn Colelli (Biology/Biochemistry ’13, Beckman Foundation)Kevin Karanja (Worcester State Chemistry, ’12, NSF)Michael Nicastri (Chemistry/Biochemistry ’13, Holy Cross)Laura Urbanski (Chemistry/Biochemistry ’13, Holy Cross)Jennie Williams (Chemistry ‘13, NSF) Summer 2010Stacy Chin (Chemistry ’12, NSF)Colleen Donahue (Biology ’11, NIH)Michelle Marieni (Biology ’11, NSF)George Savidis (Biology/Biochemistry ’12, Dreyfus)Kristina Xega (Chemistry/Biochemistry ’11, NSF) Summer 2009Lauren Duffee (Biology/Biochemistry ’10, NSF)Adam Nadelson (Biology ’10, NSF)Kevin O’Brien (Biology/Biochemistry ’11, NSF)Daniel York (Biology/Biochemistry ’10, NSF) Summer 2008Adam Nadelson (Biology ’10, NSF)Daniel York (Biology/Biochemistry ’10, NSF) Summer 2007Adam Kerrigan (Biology ’09, NSF)Melissa McGill (Biology/Biochemistry ’08, NSF)Ann Schufreider (Chemistry ’09, NSF)Jennifer Winslow (Biology ’09, NSF) Summer 2006Deirdre M. Dorval (Biology ’06, NSF)Melissa McGill (Biology/Biochemistry ’08, NSF)Kathryn O’Brien (Chemistry/Biochemistry ’08, NSF)
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Summer 2005Matthew Drago (Biology/Biochemistry ’07, NSF)Deirdre M. Dorval (Biology ’06, Research Corporation)Taryn Powers (Biology ’07, NSF) Summer 2004Deirdre M. Dorval (Biology ’06, Research Corporation)Katherine Lewandowski (Biology/Biochemistry ’05, Simeon J. Fortin Charitable Trust) Summer 2003Jaclyn Gareau (Chemistry/Biochemistry ’04, Simeon J. Fortin Charitable Trust)Katherine Lewandowski (Biology/Biochemistry ’05, Simeon J. Fortin Charitable Trust) Summer 2002Alicia M. Garcia (Biology ’03, Simeon J. Fortin Charitable Trust)Jennifer S. Manning (Chemistry ’03, Simeon J. Fortin Charitable Trust)Timothy D. Quinn (Chemistry/Biochemistry ’04, Pfizer, PREPARE)Lisa A. Wuerdeman (Chemistry ’03, Simeon J. Fortin Charitable Trust) Academic Year Research Students 20152016Christopher Cousin (Chemistry ’16) Chemistry Research IMichael Koulopoulos (Chemistry/Biochemistry ’16) Chemistry Research II/Biochemistry ThesisJoshua Long (Biology ’17) Chemistry Research IChristopher Minteer (Chemistry/Biochemistry ’17) Chemistry Research IChristian Ramsoomair (Chemistry ’18) Introduction to ResearchAnthony Yakely (Chemistry/Biochemistry ’16) Chemistry Research II/Biochemistry Thesis 20142015
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Alexander Bonano (Chemistry ‘17) ALANA Mentoring ProgramVanessa Costa (UNDL ’18) First year Research Advancement ProgramChristopher Cousin (Chemistry ’16) Introduction to ResearchZachary Giaccone (Chemistry/Biochemistry ’15) Chemistry Research II/Biochemistry ThesisMichael Koulopoulos (Chemistry/Biochemistry ’16) Chemistry Research IMario Jaramillo (Biology ’15) Chemistry Research IIChristian Ramsoomair (UNDL ’18) First year Research Advancement ProgramNicolle Siegart (Biology/Biochemistry ’15) Chemistry Research II/Biochemistry ThesisAnthony Yakely (Chemistry/Biochemistry ’16) Chemistry Research I 20132014Alexander Bonano (Chemistry ‘17) ALANA Mentoring ProgramSarah Cahn (Chemistry ’14) Chemistry Research IIZachary Giaccone (Chemistry/Biochemistry ’15) Chemistry Research IDaniel Haley (Chemistry ‘14) Chemistry Research IMario Jaramillo (Biology ’15) Chemistry Research INicolle Siegart (Biology/Biochemistry ’15) Chemistry Research I 20122013Sarah Cahn (Chemistry ’14) Chemistry Research IKathryn Colelli (Biology/Biochemistry ’13) Chemistry Research II/Biochemistry Thesis
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Michael Nicastri (Chemistry/Biochemistry ’13) Chemistry Research II/Biochemistry ThesisJennifer Pusztay (Chemistry/Biochemistry ’13) Chemistry Research II/Biochemistry ThesisNicolle Siegart (Biology ’15) Introduction to ResearchLaura Urbanski (Chemistry/Biochemistry ’13) Chemistry Research II/Biochemistry Thesis/Honors ThesisJennie Williams (Chemistry ’13) Chemistry Research II 20112012Stacy Chin (Chemistry ’12) Chemistry Research IIKathryn Colelli (Biology/Biochemistry ’13) Chemistry Research IKevin Karanja (ChemistryWorcester State ’12)Michael Nicastri (Chemistry/Biochemistry ’13) Chemistry Research IJennifer Pusztay (Chemistry/Biochemistry ’13) Introduction to ResearchGeorge Savidis (Biology/Biochemistry ’12) Chemistry Research II/Biochemistry ThesisLaura Urbanski (Chemistry/Biochemistry ’13) Chemistry Research IJennie Williams (Biology ’13) Introduction to Research 20102011Stacy Chin (Chemistry ’12) Chemistry Research IColleen Donahue (Biology ’11) Chemistry Research IMichelle Marieni (Biology ’11) Chemistry Research IKevin O’Brien (Biology/Biochemistry ’11) Chemistry Research IIGeorge Savidis (Biology/Biochemistry ’12)
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Chemistry Research IJennie Williams (Biology ’13) Introduction to ResearchKristina Xega (Chemistry/Biochemistry ’11) Chemistry Research I 20092010Lauren Duffee (Biology/Biochemistry ’10)
Chemistry Research II/Biochemistry ThesisAdam Nadelson (Biology ’10)
Chemistry Research IIKevin O’Brien (Biology/Biochemistry ’11) Chemistry Research IKristina Xega (Chemistry/Biochemistry ’11) Introduction to ResearchDaniel York (Biology/Biochemistry ’10)
Chemistry Research II/Biochemistry Thesis 20082009Lauren Duffee (Biology/Biochemistry ’10) Introduction to ResearchAdam Kerrigan (Biology ’09)
Chemistry Research IIAdam Nadelson (Biology ’10)
Chemistry Research IAnn Schufreider (Chemistry ’09)
College HonorsJennifer Winslow (Biology ’09)
Chemistry Research IIDaniel York (Biology/Biochemistry ’10)
Chemistry Research I 20072008Adam Kerrigan (Biology ’09)
Chemistry Research IMelissa McGill (Biology/Biochemistry ’08)
Chemistry Research II/Biochemistry ThesisKathryn O’Brien (Chemistry/Biochemistry ’08)
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Chemistry Research II/Biochemistry ThesisAnn Schufreider (Chemistry ’09)
Chemistry Research IJennifer Winslow (Biology ’09)
Chemistry Research I 20062007Matthew Drago (Biology/Biochemistry ’07)
College Honors/Biochemistry ThesisMelissa McGill (Biology/Biochemistry ’08)
Chemistry Research IKathryn O’Brien (Chemistry/Biochemistry ’08)
Chemistry Research ITaryn Powers (Biology ’07)
Chemistry Research II 20052006Deirdre Dorval (Biology ’06)
College HonorsMatthew Drago (Biology/Biochemistry ’07)
Chemistry Research ITaryn Powers (Biology ’07)
Chemistry Research I 20042005Deirdre Dorval (Biology ’06)
Chemistry Research IKatherine Lewandowski (Biology/Biochemistry ’05)
Chemistry Research II/Biochemistry Thesis 20032004Jaclyn Gareau (Chemistry/Biochemistry ’04)
Chemistry Research II/Biochemistry Thesis/Departmental HonorsKathleen Leahy (Mathematics ’04) College Honors (oncampus advisor)Katherine Lewandowski (Biology/Biochemistry ’05) Chemistry Research I
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Timothy Quinn (Chemistry/Biochemistry ’04) Chemistry Research II/Biochemistry Thesis/Departmental Honors 20022003Alicia Garcia (Biology ’03) College HonorsJaclyn Gareau (Chemistry/Biochemistry ’04) Chemistry Research ITimothy Quinn (Chemistry/Biochemistry ’04) Chemistry Research IJennifer S. Manning (Chemistry ’03) Chemistry Research I Ralph M. Millilo (Biology ’03) with Prof. R. Bertin Biology Research/Biochemistry ThesisLisa Wuerdeman (Chemistry ’03) Chemistry Research I 20012002Beth Ambrose (Chemistry ’02) Chemistry Research IPatrick Twomey (Chemistry/Biochemistry ’02) Chemistry Research I/Biochemistry ThesisJennifer S. Manning (Chemistry ’03) Unofficial Chemistry ResearchLisa Wuerdeman (Chemistry ’03) Unofficial Chemistry Research College Honors Program Thesis Reader Katherine Beckwith (Biology ’03), 2003Cristiana Pineda (Chemistry ’12), 2012 Selected College Service/Professional Development 2013 STEME working group, cofounder2013 Search Committee, Director of Sponsored Research20112013 Finance and Planning Council
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2013 Independent Colleges Office Liaison Meeting participant20132014 Colleges of Worcester Consortium Leadership Fellows Program2013 Ad hoc Montserrat Writing Assessment Committee2013 Ad hoc AAC subcommittee on faculty research20112012 Presidential Colloquium on Jesuits and the Liberal Arts20082010 Committee on Faculty Affairs, Chair, 200910 Subcommittee Chair, Handbook on Tenure and Promotion Subcommittee on Appeals20082009 Science Ambassadors Faculty Advisor20082010 Faculty mentorship program20082009 NEASC Review Subcommittee on Governance20082010 Faculty Mentorship Program2010 Beckman Scholars selection committee2007 “Being a Holy Cross Student” Gateways Session Leader20042006 Academic Affairs Council, Priorities Committee20042006 Curricular Review Steering Committee2001present Biochemistry Concentration, CoChair20022003, 20042007 Committee for Graduate Studies and Fellowships20022003 Curricular Goals Committee: Acquisition, integration and application of knowledge200607, 1012 Presidential Service Award Selection Committee2002 Undergraduate Summer Research Symposium, cochair20022004, 2007 Summer Gateways Advisor2002present Founder/organizer of summer biomolecular summer student research student journal club2004 Presented talk on “Mad Cows and Cannibals” for
summer research program20042006 PAL host for Atoms and Molecules2005 Ad hoc Classroom of the Future committee20032006, 2008 Organized student group trip to MIT Center for Cancer
Research Annual Symposium20022007 Founded/organized annual summer student/faculty softball
game series (w/ G. Roberts) Selected Department Service 2009 Revised and lead department seminar program as teaching overload
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20042010 General Chemistry laboratory faculty director20052007 New Chemistry building planning, committees for Material Management, General Chemistry lab, Biochemistry research and
Nonsynthetic Research20012002 Cross and Crucibles faculty advisor2001,02,05.07 Participated in Fall Parent’s Weekend Open House2004, 12 Hosted prospective student department session2003,04,07,12 Hosted departmental Gateways browsing sessions2001present Hiring committees for ten faculty and four lab coordinators2003 Cowrote Departmental Academic Honesty Policy2001 Graduate School advising session panelist2001present Biochemistry coordinator2001present Hosted prospective student summer visits to the department Selected Community Service 2014 NSF Review Panel, Division of Undergraduate Education (Apr 2014)201216 Wachusett Regional School Committee member 20122013: Subcommittees on Education and Community Outreach 2012: Davis Hill Elementary School Improvement Council 2013: Subcommittees on Management and Legal Affairs (Chair)2012 Colleges of Worcester Consortium, Faculty panelist, “Planning Successful Scholarship: Obtaining Fellowships and Grants forSabbaticals and Ongoing Research” (11/2/12)2011 PKAL Learning Spaces Collaboratory Regional Workshop Panelist2011 NSF Review Panel, Molecular and Cellular Biosciences (Apr 2011)2010 NSF Review Panel, Molecular and Cellular Biosciences (Apr 2010)2010 Chemistry demonstration to 50 46 year olds, Goddard School, Auburn, MA2010,11,14 Poster judge at undergraduate poster session, ASBMB national meeting2009 NSF Review Panel MRIPUI, Chemistry (Oct 2009)2007 Ad hoc reviewer for Protein Science, Biochemistry, National Science Foundation MCB, Chembiochem, Journal of Chemical
Education, IUBMB Life, BMC Bioinformatics, Journal of Bacteriology, PLoS One, Acta Biochimica et Biophysica Sinica,Nature Biotechnology, Nature Chemical Biology, Frontiers in Microbiology
2009 Poster session judge at Protein Society meeting2008present Founder and Advisor, Holy Cross Undergraduate Affiliate Network of American Society for Biochemistry and Molecular
Biology2006 Seminar to postdoc association at UMass Medical School on career options in liberal arts colleges2005 Participated as panelist in Science faculty forum by Consortium on High
Achievement and Success, Mt. Holyoke College2005 Presented talk to Johns Hopkins Center for Talented Youth Odyssey Series
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2005 Panelist for HHMIBoston area alumni group career options discussion20012002 United Way Fund Drive Department Coordinator2002 Relay for Disaster Relief participant Professional Membership 2008present Project Kaleidoscope, F212000present American Chemical Society, Biological Chemistry Division2000present American Society for Biochemistry and Molecular Biology2005present Protein Society2001present Council on Undergraduate Research1995present Phi Beta Kappa1995present Alpha Sigma Nu (National Jesuit Honor Society)2010present American Society for Microbiology