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8/2/2019 Katherine Taylor Microbiology Definitions 2012
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Katherine Taylor
Microbiology Vocabulary
susceptibility The likelihood of suffering from an adverse effect or disorder when exposed to anoxious stimulus or pathogen.
B Lactam antibiotics-
minimum inhibitory concentration The minimum antibiotic concentration needed to inhibit
bacterial growth from a clinical isolateeg, a bloodborne infection, which is a form of
antimicrobial susceptibility testing
minimum bactericidal concentration The lowest concentration of an antibiotic that is
bactericidal to 99.9% of an original inoculum.
physical barrier- anything that impedes your progress or movement.
chemical barrier- variety of chemicals act as nonspecific immune devices in the body. The
important ones are : lysozyme, lactoferrin, transferrin
Opsonization the rendering of bacteria and other cells subject to phagocytosis
Chemotaxic The movement of cells in response to chemical stimulant.
Aquired immunity- Immunity acquired by infection or vaccination (active immunity) or by thetransfer of antibody or lymphocytes
Antibodies Specialized cells of the immune system which can recognize organisms that invade
the body (such as bacteria, viruses, and fungi). The antibodies are then able to set off a complex
chain of events designed to kill these foreign invaders.
AntigenAny substance that produces an immune response
Innate immunity- Immunity that occurs naturally as a result of a person's genetic constitution or
physiology and does not arise from a previous infection or vaccination.
IgG antibodies- IgG antibodies are involved in predominantly thesecondary immune response.
The presence of specific IgG, in general, corresponds to maturation of the antibody response
IgM antibodies - IgM antibodies are the largest antibody. They are found in blood and lymph
fluid and are the first type of antibody made in response to an infection
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Primary Immune response - The immune response occurring on the first exposure to a foreign
material in the body.
Secondary immune response- the immune response occurring on second and subsequent
exposures to an antigen, with a stronger response to a lesser amount of antigen, and a shorter lag
time compared to the primary immune response.
Rapid Methods - the study of improved methods in the isolation, early detection,
characterization, and enumeration of microorganisms and their products in clinical, food,
industrial, and environmental samples.
Vitek- system for rapid microbial identification and antibiotic susceptibility testing.
MicroScan WalkAway is an automated bacterial identification and susceptibility testing system
that has only recently been marketed in Australasia.
Numeric codes Numeric codes in microbiology, outbreaks of a number of diseases.
Profile number- new diseases that suddenly develop into epidemics which can affect a large
number of people.
Indigenous Flora - "the usually complex mixture of bacterial population that colonizes a given
area in the host
commensal - living in a state of commensalism;noun either of two different animal or plant
species living in close association but not interdependent
carrier - an organism that possesses a recessive gene whose effect is masked by a dominant
allele; the associated trait is not apparent but can be passed on to offspring;
parasite - an animal or plant that lives in or on a host (another animal or plant); it obtains
nourishment from the host without benefiting or killing the host; a follower who hangs around a
host (without benefit to the host) in hope of gain or advantage
pathogen - any disease-producing agent (especially a virus or bacterium or other
microorganism)
opportunistic - taking immediate advantage, often unethically, of any circumstance of possible
benefit
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virulence - extreme hostility; extreme harmfulness (as the capacity of a microorganism to cause
disease)
phagocytosis - process in which phagocytes engulf and digest microorganisms and cellular
debris; an important defense against infection
Lysosomes
chemotaxis - movement by a cell or organism in reaction to a chemical stimulus
inflammation - arousal to violent emotion; a response of body tissues to injury or irritation;
characterized by pain and swelling and redness and heat; the act of setting something on fire; the
state of being emotionally aroused and worked up
host - an animal or plant that nourishes and supports a parasite; it does not benefit and is often
harmed by the association
Exotoxins
Endotoxins
invasion - the act of invading; the act of an army that invades for conquest or plunder;
(pathology) the spread of pathogenic microorganisms or malignant cells to new sites in the body;
any entry into an area not previously occupied
dissemination - the property of being diffused or dispersed; the opening of a subject to
widespread discussion and debate; the act of dispersing or diffusing something
zoonoses.
epidemiology - noun the branch of medical science dealing with the transmission and control of
disease
endemic - adj. native to or confined to a certain region; of or relating to a disease (or anything
resembling a disease) constantly present to greater or lesser extent in a particular locality ;
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epidemic a widespread outbreak of an infectious disease; many people are infected at the same
time
polymicrobic marked by the presence of several species of microorganisms
etiologic agents microorganisms and microbial toxins that cause disease in humans and includebacteria, bacterial toxins, viruses
standard precautions guidelines recommended by the Centers for Disease Control and Prevention
for reducing the risk of transmission of blood-borne and other pathogens in hospitals.
non-invasive specimen
invasive specimen
direct examination
centrifuged sediment smear
enrichment broth one modified to permit growth by selected bacteria. An example is selenite
broth, which is selective for salmonellae.
Purelence
Gram Stain a staining procedure in which microorganisms are stained with crystal violet, treated
with strong iodine solution, decolorized with ethanol or ethanol-acetone, and counterstained with
a contrasting dye; those retaining the stain aregram-positive, and those losing the stain but
staining with the counterstain aregram-negative.
Wright Giemsa stain a solution containing azure II-eosin, azure II, glycerin, and methanol; used
for staining protozoan parasites, such asPlasmodiumandTrypanosoma, forChlamydia, for
differential staining of blood smears, and for viral inclusion bodies.
Ziel Neelson Stain Acid-fast stain (Ziehl-Neelsen method) is a useful differential staining
procedure that specifically stains all members of the genera mycobacteria
Methylene Blue Stain Methylene blue stain is a general biological stain used to highlight parts
of animal, bacteria and blood tissue specimens.
Dna Probe An agent that binds directly to a predefined sequence of nucleic acids
Amorphous debris Small / Moderate / Large amounts of. Red, white and epithelial cells. Any
number of red blood cells is abnormal in a urine deposit
Colonial morphology is a way scientists can identify bacteria
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Lactose fermenter
Non fermenter
Hemolysis is the breakdown of red blood cells
Alpha hemolysis is a greenish discoloration that surrounds a bacterial colony
Beta Hemolys is sometimes called complete hemolysis, is a complete lysis of red cells in the
media around and under the colonies: the area appears lightened (yellow) and transparent
Streamers a stream of light, especially one appearing in some forms of the aurora borealis
Puff Balls any of various basidiomycetous fungi, especially of the genusLycoperdon and allied
genera, characterized by a ball-like fruit body that emits a cloud of spores when broken.
Turbidity not clear or transparent because of stirred-up sediment
Catalase an enzyme that decomposes hydrogen peroxide into oxygen and water
Coagulase anyenzyme that causes coagulationofblood
Coagulase negative staphylococci
Protein a
Furnucles is a boil which has infected a hair follicle
Carbuncles an abscess larger than a boil, usually with one or more openings draining pus onto
the skin.
Osteomyelitis infection of the bone marrow
Novobiocin susceptibility is determined by placing a novobiocin-impregnated paper disk on a
nutrient agar plate seeded with the microbe under investigation.
Beta lactamase any of a group of enzymes, produced by almost all gram-negative bacteria, that
hydrolyze the -lactam ring of penicillins and cephalosporins, destroying their antibiotic activity.
Methicillin Resistant Staphylococci is a bacterium responsible for several difficult-to-treat
infections in humans.
Ritters Diseasepathological condition of a part, organ, or system of an organism resulting from
various causes, such as infection, genetic defect, or environmental stress
Streptococcus spherical Gram-positive bacteria occurring in pairs or chains; cause e.g. scarlet
fever and tonsillitis
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Enterococcus is a genus of lactic acid bacteria of the phylum Firmicutes
Lancefield Classification the systematic arrangement of similar entities on the basis of certain
differing characteristics
Group A Streptococci is the causative agent in Group A streptococcal infections,
Streptococcus pyogenes Streptococcus pyogenes is a spherical, Gram-positive bacterium that is
the cause of group A streptococcal infections.
Group B Streptococci also known as 'Streptococcus agalactiae' and more colloquially as Strep B
and group B Strep, can cause serious illness and sometimes death, especially in newborn infants,
the elderly, and patients with compromised immune systems
Bacitracin a polypeptide antibiotic of known chemical structure effective against several types of
Gram-positive organisms; usually applied locally
Sulfamethoxazole and trimethoprim a sulfonamide (trade name Gantanol) used to treat infections
(especially infections of the urinary tract
Optochin is a chemical used in cell culture techniques for the presumptive identification of
Streptococcus pneumoniae
Bile Solubility A modified bile solubility test is described which can be used to presumptively
identify Streptococcus pneumoniae recovered in blood culture
Strep pneumonia is a Gram-positive, alpha-hemolytic, aerotolerant anaerobic member of the
genus Streptococcus
Hippurate Hydrolysis a biochemical reaction used to identify bacteria. Particularly used for
differentiation of streptococci causing bovine mastitis
PYR hydrolysis test a preliminary identification for Streptococci
CAMP testis a test to identify Group B -streptococci based on their formation of a substance
(CAMP factor) that enlarges the area of hemolysis
Bile esculin hydrolysis test To isolate and identify bacteria able to hydrolyze esculin in the
presence of bile. Commonly used for presumptive identification of group D streptococci and
enterococci, all of which are positive
NACL test
M protein (Streptococcus), a virulence factor of the bacterium Streptococcus pyogenes
Hyaluronidase (spreading factor) This is also called the spreading factor because it catalyzes
the breakdown of hyaluronic acid, the substance that cements the human cells
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Streptolysin O is a bacterial protein that forms an alpha helix and is toxic to eukaryotic cells. Its
toxic effect is due to the protein's ability to bind
pharyngitis - inflammation of the fauces and pharynx
Scarlet fever is a rash that is caused by strep infections
.
impetigo - a very contagious infection of the skin; common in children; localized redness
develops into small blisters that gradually crust and erode
erysipelas - an acute streptococcal infection characterized by deep-red inflammation of the skin
and mucous membranes
cellulitis - an inflammation of body tissue (especially that below the skin) characterized by feverand swelling and redness and pain
rheumatic fever is an inflammatory disease that may develop after an infection with
Streptococcus bacteria
Acute Glomerulonephritis is a group of synptoms that occur with some disorders that cause
glomerulonephritis, or swelling and inflammation of the glomeruli in the kidney.
Streptoccoal toxic shock syndrome is a rare but extremely severe illness characterized by
hypotension (low blood pressure) and shock
Group D streptococci Group D streptococci have been reclassified and placed in the genus
Enterococcus(includingEnterococcus faecalis,Enterococcus faecium,Enterococcus durans,
andEnterococcus avium
Corynebacterium sp. is agenusofGram-positive, rod-shapedbacteriaresponsible for causing
diphtheria.
Diptheria toxin is an exotoxin secreted by Corynebacterium diphtheriae, the pathogen
bacterium that causes diphtheria
Arcanobacterium formerly known as Corynebacterium hmolyticum, is a bacterial species.
Listeria monocytogenes facultative anaerobe, intracellular bacterium, is the causative agent of
listeriosis. It is one of the most virulent foodborne pathogens
Tumbling motility A motile bacteria propels itself from place to place by rotating its flagella.
To move forward, the flagella rotate counterclockwise and the organism "swims
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Erysipelothrix rhusiopathiae is a gram-positive, catalase-negative, rod-shaped bacterium. It
grows aerobically and anaerobically and does not contain endotoxin
Bacillus is agenusofGram-positive, rod-shapedbacteriaand a member of the division
Firmicutes.Bacillus species can be obligateaerobesorfacultative anaerobes, and test positive
for theenzymecatalase
Anthrax is an infectious disease due to a type of bacteria called Bacillus anthracis
Nocardia is agenusof weakly stainingGram-positive,catalase-positive, rod-shapedbacteria. It
forms partiallyacid-fastbeaded branching filaments
Actinomadura is a filamentous bacterium found in soil. Actinomadura is an aerobic
actinomycetes
Streptomyces is the largest genus of Actinobacteria and the type genus of the family
Streptomycetaceae
TheNeisseria is a large genus ofcommensalbacteriathat colonize themucosalsurfaces of many
animals. Of the 11 species that colonize humans, only two arepathogens
Moraxella catarrhalis is a fastidious, nonmotile,Gram-negative,aerobic,oxidase-positive
diplococcusthat can causeinfectionsof therespiratory system,middle ear,eye,central nervous
systemandjointsof humans
Haemophilus is a genus of Gram-negative, pleomorphic, coccobacilli bacteria belonging to the
Pasteurellaceae family
Hematin is considered the "X factor" required for the growth of Haemophilus influenza
Factor V (pronounced factor five) is aproteinof thecoagulationsystem, rarely referred to as
proaccelerin or labile factor. In contrast to most other coagulation factors, it is not
enzymatically active but functions as acofactor
Satellitism the phenomenon in which certain bacterial species grow more vigorously in the
immediate vicinity of colonies of other unrelated species, owing to the production of an essential
metabolite by the latter species
Encapsulated enclosed in fibrous or membranous sheaths. It refers to organisms that form aprotective capsule.
Nonencapsulated It refers to organisms that do not form a protective capsule.
Chanchroid is also referred to as a venereal ulcer. It is caused by the Haemophilus ducreyi
bacillus
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Pink eye Conjunctivitis is swelling (inflammation) or infection of the membrane lining the
eyelids (conjunctiva)
Buboes is swelling of the lymph nodes. It is found in infections such as bubonic plague,
gonorrhea, tuberculosis
School of Fish Appearance descriptive term for a light microscopic pattern characterised by
multiple, discrete oval-to-elongated structures arranged in long, roughly parallel fascicles
Legionellosis is a potentially fatal infectious disease caused by gram negative, aerobic bacteria
belonging to the genus Legionella
Legionnaires' disease acquired its name in July 1976 when an outbreak of pneumonia occurred
among people attending a convention of the American Legion
Pontiac fever flu-like illness caused by the bacterium Legionella pneumophila contracted by
breathing mist that comes from a water source
Fluorescein isoothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging
applications including flow cytometry.
L cysteine a sulfur-containing, nonessential amino acid produced by enzymatic or acid
hydrolysis of proteins
Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or
grow certain bacteria, particularly the Gram-negative species
The Enterobacteriaceae is a large family of Gram-negative bacteria that includes many of themore familiar pathogens, such as Salmonella, Escherichia coli
Enterics rod-shaped gram-negative bacteria of the family Enterobacteriaceae ; most occur
normally or pathogenically in intestines of humans and other animals
O antigen repetitiveglycanpolymercontained within an LPS is referred to as the Oantigen, O
polysaccharide, or O side-chain of the bacteria. The O antigen is attached to the core
oligosaccharide, and comprises the outermost domain of the LPS molecule
H antigen a bacterial flagellar antigen important in the serological classification of enteric bacilli.
the precursor of the A and B blood group antigens; normal type O individuals lack the enzyme toconvert it to A or B antigens
K antigen a bacterial capsular antigen, a surface antigen external to the cell wall
E. Coli is a Gram-negative, rod-shaped bacterium that is commonly found in the lower intestine
of warm-blooded organisms (endotherms).
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Shiga toxin also known as verotoxin, is a toxin generated by some strains of Escherichia coli
Triple Sugar Iron medium is a differential medium that can distinguish between a number of
Gram-negative enteric bacteria based on their physiological ability (or lack thereof) to:
Kliger iron agar KIA are used to determine if bacteria can ferment glucose and/or lactose and if
they can produce hydrogen
Sulfid, indole, motility (SIM) medium is a semisolid agar used to determine hydrogen sulfide
(H2S) production, indole formation, and motility
Motility, indole, ornithine (MIO) Medium is designed to aid in the identification of members of
the family Enterobacteriaceae on the basis of motility
Beta galactosidase also called beta-gal or -gal, is ahydrolaseenzymethatcatalyzesthehydrolysisof
-galactosidesintomonosaccharides
Beta galactosidase permease catalyzes the hydrolysis of its disaccharide substrate via "shallow"
and "deep" binding, thus breaking down lactose
MRVP a differential test for bacterial respiration, used to distinguish/differentiate strainsof coliform bacteria (i.e., Escherichiaand Enterobacter) methyl red test contains a smallamount of carbohydrate fermentable by both organisms.
Indole A white crystalline compound obtained from coal tar or various plants and found in the intestines
and feces as a product of the bacterial decomposition of tryptophan. Also called ketole.
Deaminase Any of a class of enzymes that catalyze the hydrolysis of compounds containing an amino
group. Also called deaminating enzyme.
Decarboxlyase is a chemical reaction that releases carbon dioxide (CO2)
Nitrate Reduction Nitrate broth is used to determine the ability of an organism to reduce nitrate
(NO3) to nitrite (NO2) using the enzyme nitrate reductase.
Vibrio sp Cholera is an infection of the small intestine that causes a large amount of watery
diarrhea. Symptoms: Abdominal cramps; Dry mucus membranes or mouth; Dry skin; Excessive
thirst; Glassy or sunken eyes; Lack of tears
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Aeromonas sp. Aeromonas is a gram-negative, facultative anaerobic rod that morphologically
resembles members of the family Enterobacteriaceae
Plesimonas sp shigelloides is a species of bacteria. It is a Gram-negative, rod-shaped
bacterium which has been isolated from freshwater, freshwater fish
Campylobacter sp. is a genus of bacteria that are Gram-negative, spiral, and microaerophilic.
Motile, with either unipolar or bipolar
Campy
Darting motility A motile bacteria propels itself from place to place by rotating its flagella
Urea Breath test The urea breath test is a rapid diagnostic procedure used to identify infections
by Helicobacter pylori, a spiral bacterium implicated in gastritis, gastric ulcer,
Asaccharolytic incapable of metabolizing glucose or other carbohydrates
Pseudomembranous colitis Pseudomembranous colitis
Aerotolerance test the first biochemical test used for the identification of aerobic gram positive
bacteria; catalase
Presumptive identification identification by the colony morphology, growth on selective media,
gram stains
Clostridium sp is a genus of Gram-positive bacteria, belonging to the Firmicutes. They are
obligate anaerobes capable of producing endospores
Egg-Yolk Agar (EYA) is an enriched non-selective and differential medium for the presumptive
identification of certain anaerobic isolates
Actinomyces spp Greek "actis" ray, beam and "mykes" mucus, fungus, is a genus of the
actinobacteria class of bacteria
Bifidobacterium is a genus of Gram-positive, non-motile, often branched anaerobic bacteria.They are ubiquitous, endosymbiotic inhabitants
Eubacterium a genus of bacteria of the family Propionibacteriaceae, found as saprophytes in
soil and water
Mobiluncus is a genus of gram-positive, anaerobic, rod-shaped bacteria. While this species of
bacterium possesses a cell wall
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Lactobacillus also called Dderlein's bacillus, is a genus of Gram-positive facultative anaerobic
or microaerophilic rod-shaped bacteria
Propionibacterium sp. acnes is a relatively slow growing, typically aerotolerant anaerobic gram
positive bacterium (rod) that is linked to the skin condition acne
Petostreptococcus sp. a genus of gram-positive anaerobic chemoorganotrophic bacteria that occur in
pairs or chains. The potentially pathogenic organisms are found in normal and pathologic female genital
tracts and in the intestinal and respiratory tracts of normal humans. They have been associated with a
variety of disorders ranging from appendicitis to putrefactive wounds.
Gardnerella vaginalis is a facultatively anaerobic Gram-variable rod that can causebacterial vaginosis
in some women as a result of a disruption in the normalvaginalmicroflora
Spirochetes belong to a phylum of distinctive Gram-negative bacteria, which have long, helically
coiled (spiral-shaped) cells
Periplasmic flagella Although spirochete periplasmic flagella have many features similar to typical
bacterial flagella, they are unique in their structure and internal periplasmic location. This location
provides advantages for pathogenic spirochetes to enter and to adapt in the appropriate host
Fletchers semisolid medium Fletcher's Medium is an enriched, semisolid medium used for
the cultivation of Leptospira
Stuart liquid medium Liquid Stuart & Liquid Amies transport medium are designed to support a wide range of bacteria
Leptospirosis is a rare and severe bacterial infection that occurs when people are exposed tocertain environments
Weils Disease The disease was first described by AdolfWeil in 1886 when he reported an"acute infectious disease with enlargement of spleen, jaundice, and nephritis
Kelly medium
Arthropod vectors The deer tick, a vector for lyme disease. Arthropods form a major group of
disease vectors with mosquitoes, flies
Lyme borreliosis is an emerging infectious disease caused by at least three species of bacteriabelonging to the genus Borrelia
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Erythema chronicum migrans refers to the rash often (though not always) seen in the earlystage of Lyme disease.
Rapid plasma reagin is a screening test for syphilis. It looks for antibodies that are present in theblood of people who have the disease
Syphilis is a sexually transmitted infection caused by the spirochete bacterium Treponemapallidum subspecies pallidum
Chancre is a painless ulceration (sore) formed during the primary stage of syphilis. Thisinfectious lesion forms approximately 21 days
Gummas is a soft, non-cancerous growth resulting from the tertiary stage of syphilis. It is a formof granuloma
Mha-tp test The MHA-TP is used to confirm a syphilis infection after another method tests
positive for the syphilis bacteria
Yaws is a long-term (chronic) infection that mainly affects the skin, bones, and joints.Symptoms: About 2 - 4 weeks after infection, the person develops a sore called a "mother yaw"where bacteria entered the skin
Pinta is a human skin disease endemic to Mexico, Central America, and South America. It iscaused by infection with a spirochete, Treponema pallidum
Bejel form of endemic (nonvenereal) syphilis occurring among Bedouin tribes and elsewhere inthe Middle East
Mycoplasma sp refers to a genus of bacteria that lack a cell wall. Without a cell wall, they areunaffected by many common antibiotics such as penicillin
Ureaplasma sp is a genus ofbacteriabelonging to the familyMycoplasmataceae. As the name imples,ureaplasma is urease positive
Mycobacterium sp is agenusofActinobacteria, given its own family, the Mycobacteriaceae. The genusincludespathogensknown to cause serious diseases in mammals, includingtuberculosis
(Mycobacterium tuberculosis) andleprosy(Mycobacterium leprae
Acid fastness is a physical property of certain bacteria, specifically their resistance to
decolorization by acids during staining procedures
Nontuberculosis mycobacteria (NTM) NTM lung disease is a devastating chronic illness. NTMbacteria exist naturally in our environment
Kinyoun stain The Kinyoun stain is a method of staining acid-fast microorganisms, specificallymycobacterium
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Fluorochrome stains a fluorescent dye used to stain the cell walls of fungi and bacteria. Theorganisms then fluoresce when exposed to UV light rays
Lowenstein-Jensen The LowensteinJensen medium, more commonly known as LJ medium,is a growth medium specially used for culture of Mycobacterium
Middlebrook 7H10 and 7H11 agars Middlebrook 7H11//7H11 Selective Agar bi-plates provide two
media, one moderately selective and one highly selective, for the recovery of mycobacteria from
specimens containing mixed flora. The Middlebrook 7H11 Agar is contained in the sector differentiated
by the Roman numeral "I" molded into the bottom of the sector. The 7H11 Selective Agar is contained
in the sector marked "II."