JOURNAL OF BACTERIOLOGYVOLUME 107 * NUMBER 3 * SEPTEMBER 1971

EDITORIAL BOARD

L. LEON CAMPBELL, Editor-in-Chief(1975)University ofIllinois, Urbana

R. L. ANDERSON, Editor (1975) ROY CURTISS III, Editor (1974)Michigan State University, East Lansing The University of Tennessee, Oak Ridge

S. G. BRADLEY, Editor (1974) ALLEN G. MARR, Editor (1973)Virginia Commonwealth University, Richmond University of California, Davis

Martin Alexander (1972)W. Lane Barksdale (1972)M. E. Bayer (1972)Claire M. Berg (1973)Robert W. Bernlohr (1973)Allan Campbell (1972)A. J. Clark (1972)Royston C. Clowes (1972)V. W. Cochrane (1972)Stanley N. Cohen (1973)S. F. Conti (1972)A. L. Demain (1972)John A. DeMoss (1972)Walter J. Dobrogosz (1973)Martin Dworkin (1973)Alan D. Elbein (1973)Herman Friedman (1972)A. T. Ganesan (1972)Howard Goldfine (1972)Joseph S. Gots (1972)Harlyn 0. Halvorson (1972)F. M. Harold (1973)

Leonard Hayflick (1972)George Hegeman (1971)Charles E. Helmstetter (1972)Joseph T. Holden (1972)John G. Holt (1972)Stanley C. Holt (1971)John Ingraham (1973)John H. Law (1971)Hillel S. Levinson (1972)W. R. Lockhart (1972)Donald G. Lundgren (1971)Manley Mandel (1972)Paul Margolin (1972)Alvin Markovitz (1973)Julius Marmur (1971)Robert G. Martin (1971)M. L. Morse (1972)Richard P. Novick (1973)Leo Parks (1973)R. J. Phaff (1972)Allen T. Phillips (1973)Jack Preiss (1971)Charles C. Remsen III (1973)

William S. Reznikoff (1973)H. V. Rickenberg (1973)Antonio H. Romano (1973)John Roth (1973)M. R. J. Salton (1972)Moselio Schaechter (1973)David Schlessinger (1971)Simon Silver (1973)W. R. Sistrom (1972)Lucille Smith (1972)Bernard S. Strauss (1972)Noboru Sueoka (1972)Paul S. Sypherd (1971)A. L. Taylor (1971)H. E. Umbarger (1973)Arthur Weissbach (1971)N. E. Welker (1973)R. W. Wheat (1972)Helen R. Whiteley (1972)Meyer J. Wolin (1973)Barbara E. Wright (1971)Frank E. Young (1973)Stanley A. Zahler (1971)

ROBERT A. DAY, Managing Editor, 4715 Cordell A ve., Bethesda, Md. 20014

EX OFFICIOMORRIS F. SHAFFER, President (1971-1972)

DONALD E. SHAY, Secretary

The Journal of Bacteriology, a publication of the American Societyfor Microbiology, 4715 Cordell Ave., Bethesda, Md. 20014, is devoted tothe advancement and dissemination of fundamental knowledge concerningbacteria and other microorganisms. The Journal is published monthly, andthe twelve numbers are divided into four volumes per year. The subscrip-tion price is $52 (Postal Union Countries, $54; other foreign, $55) peryear. Single copies are $5.00 (foreign, 5.25). Members of the AmericanSociety for Microbiology may receive the Journal as part of their dues.Correspondence relating to reprints, defective copies, availability of backissues, lost or late proofs, disposition of submitted manuscripts, andgeneral editorial matters should be directed to the ASM PublicationsOffice, 4715 Cordell Ave., Bethesda, Md. 20014. Correspondence fromASM members relating to membership dues, member subscriptions,

R. G. E. MURRAY, Vice-President (1971-1972)T. J. CARSKI, Treasurer

changes of address, incorrect journals, etc., should be directed to theAmerican Society for Microbiology, 1913 St., N.W., Washington, D.C.20006. Nonmembers of the Society should write to The Williams & Wil-kins Co., 428 E. Preston St., Baltimore, Md. 21202, regarding institutionof nonmember, subscriptions and nonreceipt of journals. Publishedmonthly by the ASM at 428 E. Preston St., Baltimore, Md. 21202.

Second class postage paid at Baltimore, Md. 21202, and at additionalmailing offices.Made in the United States of America.Copyright) 1971, American Society for MicrobiologyAll Rights Reserved.

AUTHOR INDEXAmes, Bruce N., 578Andreesen, J. R., 570Arcement, Larry J., 580Augier, J., 548

Bender, R., 570Beretta, Maria, 415Betti, Mirella, 415Boisvert, H., 548Boman, Hans G., 420Bondi, Amedeo, 499Boyd, John W., 461Breitman, T. R., 535Burgan, Murrie W., 433Byers, B. R., 491

Camakaris, J., 406Campbell, L. Leon, 543Carr, N. G., 583Chang, George W., 578Cook, Thomas M., 528Cooper, Alan D., 433

Dahl, Rolf, 513Davis, Bernard D., 391Davis, C. L., 519Davis, W. B., 491Demain, Arnold L., 456Dempsey, Walter B., 580

Ennis, Herbert L., 486Evans, M. C. W., 574

Ferguson, Donald A., Jr., 461

Gancedo, Carlos, 401Genther, Clara S., 576Gibson, F., 557Gottschalk, G., 570Granato, Paul A., 551

Hachisuka, Y., 442Hahn, Fred E., 528Hammel, Jay M., 499Herdman, M., 583Herrmann, Robert L., 433Hollom, S. E., 505

Jackson, Robert W., 551Johnson, B. F. 468, 476Jolles, P., 548Jonsson, Staffan, 420

Kisliuk, Roy L., 576

Lester, Gabriel, 448Luke, R. K. J., 557

Mandel, Manley, 563Marucci, A. Alvin, 461Migliore, D., 548Monner, David A., 420Morse, M. L., 513

Olenick, John G., 528

Pace, B., 543Pace, N. R., 585Peterson, R. L., 585Phillips, Arthur W., 461Pittard, J., 406Polsinelli, Mario, 415

Radcliffe, C. W., 585Ron, Eliora Z., 391, 397Roszmann, John H., 576Rupp, W. D., 505

Shani, M., 397Smith, Carl C., 576Smith, R. V., 574Solomon, L. R., 535Sonstein, Stephen A., 499Stanier, R. Y., 468, 476Straus, Daniel, 578

Telfer, A., 574Tochikubo, K., 442

Wang, Richard J., 513Watson, Stanley W., 563White, Catherine W., 433White, Raymond F., 456Wilkins, B. M., 505Williams, L. S., 589

Yem, D. W., 589Yokoyama, M. T., 519

INDEX TO ADVERTISERS*AMERICAN OPTICAL Co....................... 13

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* Sustaining Member, American Society for Microbiology

AUTHOR INDEX

VOLUME 107

Akagi, J. M., 375Allen, Richard D., 295Ambrose, Carol A., 712Ames, Bruce N., 578Anastasio, Kathryn L., 143Andreesen, J. R., 570Andrews, S., 798Arcement, Larry J., 580Augier, J., 548

Ballesta, J. P. G., 251Bauer, S., 121Baumann, Linda, 268Baumann, Paul, 268, 295Beaman, T. Cabrera, 320Becker, Yechiel, 593Beebe, James L., 704Bender, R., 570Beretta, Maria, 415Berndtova, Lubica, 16Betti, Mirella, 415Beverin, Susan, 79Biely, P., 121Birnboim, H. C., 659Boisvert, H., 548Boman, Hans G., 420Bondi, Amedeo, 499Bott, T. L., 303Boyd, John W., 461Boyle, J. M., 61Bragg, P. D., 664Breitman, T. R., 535Brent, P., 203Brock, M. L., 303Brock, T. D., 303Brooks, David, 223Brooks, E. Lucille, 683Brown, D. J., 599Burgan, Murrie W., 433Burtner, Carolyn P., 375Byers, B. R., 491

Cagle, G. D., 315Camakaris, J., 406Campbell, L. Leon, 543Carr, N. G., 365, 583Carstensen, Edwin L., 106Chang, George W., 578Cheng, K.-J., 325Chibata, Ichiro, 741, 824Clark-Walker, G. D., 114Clowes, Royston C., 633, 900Cole, Roger M., 907Cole, Ronald S., 846Comp, Philip C., 162Cook, Thomas M., 528Cooper, Alan D., 433Costerton, J. W., 325

Couture, Jaque, 368Curtiss, Roy, III, 21

Dahl, Rolf, 513Davidson, B. E., 798Davis, Bernard D., 391Davis, C. L., 519Davis, W. B., 491DeBusk, A. Gib, 840De Lacy, Margaret L., 712Demain, Arnold L., 456Dempsey, Walter B., 580Domer, Judith E., 870Dopheide, T. A. A., 798Dorfman, Ben-Zion, 646Doudoroff, M., 690Duckworth, Donna Hardy, 259Duguid, J. P., 655Dyke, K. G. H., 68

Edwards, Merilyn J., 599Edwards, M. R., 303Elbein, Alan D., 217Englesberg, Ellis, 34Ennis, Herbert L., 486Evans, M. C. W., 574

Falkow, Stanley, 372Fenwick, Raymond G., Jr., 21Ferguson, Donald A., Jr., 461Finnerty, W. R., 806Fitz-James, P. C., 337Flavell, Richard B., 853Forsgren, Arne, 245Foulds, John, 833Fox, Maurice S., 889Freedman, M. L., 95Friedman, Solomon, 368

Gage, Vivian, 815Gancedo, Carlos, 401Genther, Clara S., 576Gerhardt, Philipp, 106, 320, 718Gibson, F., 557Goldring, Elizabeth S., 377Goodman, Robert N., 361Gordon, P. A., 114Gormus, B. J., 150Gottschalk, G., 570Granato, Paul A., 551Greenawalt, J. W., 926Grigg, G. W., 599Gross, Trevor S., 1Grossman, Lawrence I., 377Guay, Roger, 368Guerry, Patricia, 372

Hachisuka, Y., 442

Hahn, Fred E., 528Hammel, Jay M., 499Hansen, P. Arne, 382Haseltine, Florence P., 889Hatefi, Y., 223Hausmann, Celma, 633, 900Herdman, M., 583Herman, A. I., 371Herrmann, Robert L., 433Hoare, Derek S., 187Hollom, S. E., 505Hood, W., 365Huang, Pi-yu, 361

laccarino, Maurizio, 828Ingram, J. M., 325Itoh, Takuzi, 168

Jackson, Robert W., 551Jacobs, J. M., 203Jacobs, N. J., 203Johnson, B. F., 468, 476Johnston, L. H., 68JollMs, P., 548Jonsson, Staffan, 420

Kaji, Akira, 53Kaltwasser, H., 780Kato, Masahiko, 746Katz, Leonard, 34Kim, I. C., 664Kisliuk, Roy L., 576Kisumi, Masahiko, 741, 824Komatsubara, Saburo, 741, 824Kova'ri'k, J., 121Kratky, Z., 121Krishnapillai, V., 155Kubitschek, H. E., 95

Lakshmanan, Sitarama, 382Landm an, Otto E., 345Lavin, Karen, 671Lester, Gabriel, 162, 193, 448Lillich, Thomas T., 790Linder, Regina, 230Lomax, Christopher A., 1Luke, R. K. J., 557

McCuen, R. W., 636McDonald, Thomas L., 864McMurrough, I., 753MacPhee, D. G., 155Maitra, P. K., 759Makula, R. A., 806Mallavia, L., 864Mandel, M., 268, 563Margulies, Lola, 610Marmur, Julius, 377

AUTHOR INDEX

Marquis, Robert E., 106Marr, Allen G., 210Marucci, A. Alvin, 461Matin, Abdul, 179Mattern, Ina E., 8Matula, Mike, 217Mays, Laura L., 223Meyer, Ernest A., 770Michaels, George A., 385Migliore, D., 548Mirelman, David, 239Mitchell, Mike, 217Monner, David A., 420Mo Rho, Hyune, 840Morimyo, M., 623Morse, M. L., 513Mount, David W., 388

Nakata, Atsuo, 683Nauman, Robert K., 358Nordstrom, Kristina, 245Novotny, Charles P., 671

Ohnishi, Yoshinari, 918Old, D. C., 655Olenick, John G., 528Ordal, Z. John, 134Osawa, Syozo, 168Otaka, Eiko, 168

Pace, B., 543Pace, N. R., 585Palleroni, N. J., 690Pankratz, H. Stuart, 320Park, James T., 239Park, Sang Shin, 79Patch, Cephas T., 345Patel, Ramesh N., 187Paterson, M. C., 61Pearce, Susanne M., 337Peterson, George R., 683Peterson, R. L., 585Philipson, Lennart, 245Phillips, Arthur W., 461Pittard, A. J., 8, 406, 736Pollock, Jerry J., 230

Polsinelli, Mario, 415Popkin, Terry J., 907Porter, John F., 150Prabhakaran, Kochukunju, 787Premakumar, R., 100Prestidge, Louise, 815

Radcliffe, C. W., 585Ranu, Rajinder Singh, 53Reeve, J. N., 926Remeza, Virgilija, 599Repaske, Anne C., 712Repaske, Roy, 712Rittenberg, Sydney C., 179Ron, Eliora Z., 391, 397Roozen, Kenneth J., 21Rose, A. H., 753Roszmann, John H., 576Rothman, Frank G., 683Rudner, Rivka, 610Rupp, W. D., 505Russell, R. R. B., 736

Salton, Milton R. J., 230Sarov, Israel, 593Schaechter, M., 251Scherrer, Rene, 718Sedliakovi, Milena, 16Setlow, R. B., 61Shani, M., 397Shaw, D. R. D., 239Shaw, N., 130Shehata, Talaat E., 210Sheppard, David E., 79Shimazu, Y., 623Silver, Marvin, 368Silverman, David J., 358Simonds, Josephine, 382Simpson, R. J., 798Sjoquist, John, 245Smith, Carl C., 576Smith, R. V., 574Solomon, L. R., 535Sonstein, Stephen A., 499Soucheck, J. A., 143Spizizen, John, 815

Sreeleela, N. S., 100Stanier, R. Y., 468, 476Stead, D., 130§tefunkovA, Eva, 16Stewart, P. R., 114Stocker, B. A. D., 155Straus, Daniel, 578Subba Rao, P. V., 100Sugiura, Masaki, 741Sugiyama, H., 143Suzuki, K., 623

Tanguay, Robert, 368Theodore, Theodore S., 907Telfer, A., 574Thorne, C. B., 636Tochikubo, K., 442Tomlins, Richard I., 134Treiber, Gerda, 828Trivett, Terrence L., 770

Vaidyanathan, C. S., 100Vela, G. R., 315Voelz, Herbert, 358von Meyenburg, Kaspar, 878

Walker, R. T., 618Wang, Richard J., 513Watson, Stanley W., 563Weigand, R. A., 926Welker, N. E., 697Wheat, Robert W., 150White, Catherine W., 433White, David C., 790White, Raymond F., 456Wilkins, B. M., 505Williams, L. S., 589Winkler, Herbert H., 74, 259Woods, Robin A., 1Woodward, Dow O., 853Wright, Michel, 87

Yem, D. W., 589Yokoyama, M. T., 519Young, Frank E., 223

* @

J. BACTERIOL.

SUBJECT INDEX

VOLUME 107

A componentof S. zymogenies lysin, 551

-physical and chemical characterization of, 551purification of, 551

ade3 locus- in S. cerevisiae, 1ade3 mutants of yeast, 1Adenine-requiring mutants- in S. cerevisiae, 1Alcaligentes eutrophus- dissimilation of aromatic compounds by, 468- j3-ketoadipate pathway in, 468, 476Allantoin-- aerobic metabolization by B. fastidiosus, 780Allelic complementation- in S. cerevisiae, 646Ampicillin-resistant mutants- of E. coli K-12, 420Anaerobic growth- in M. genevensis, 114Anthranilate degradation- in A. iniger, 100- regulation of, 100Antibiotics- mutants of E. coli sensitive to, 486L-Arabinose operon- D-fucose, gratuitous inducer of, 79- gratuitous induction of, 79- in E. coli, 79ara operon- self-catabolite repression in, 34Xarog+ transductant- of E. coli, 798Aromatic compounds- dissimilation by A. eutrophus, 468Arthrobacter species- lipid composition of, 130L-Asparaginases- from S. marcescens and E. coli, 461- immunochemical comparison of, 461Aspergillus niger- anthranilate degradation in, 100Asymmetric template function- of microbial DNA, 610

Bacillus cereus spores-physical state of electrolytes within, 106-physical state of water within, 106Bacillus fastidiosus-physiology of, 780Bacillus licheniformis-competence for transformation in, 636--genetic mapping in, 636-peptide mutations in, 636Bacillus megaterium-action of 2-hydroxy-3-(cyclchexylpropyl)-1, 4-naph-thoquinone on, 528

-active transport of iron in, 491-molecular sieving by cell wall and protoplast, 718Bacillus stearothermophilus-cell wall structure, 697-rRNA of, 543Bacillus subtilis-glycerol-requiring mutant of, 790-inactive glucose dehydrogenase from, 442-mRNA transcription, 610-phospholipid metabolism in, 790-protease activities in, 815-ribosomes of, 53-sporulation in, 815Bacillus subtilis mutant-phosphatidylethanolamine-deficient, 704Bacteriocin-actions of, 143-boticinogeny of, 143Bacteriocin JF 246-from S. marcescenis, 833-inhibition of macromolecular synthesis by, 833Bacteriophage lam6da-inactivation by psoralen plus 360-nm light, 846Bacteriophage-like particles-in E. coli, 926-inhibitory, 926Bacteriophage lytic enzyme--effect on B. stearothermophilus cell walls, 697Betaine requirement-for porphyrin overproduction in P. denzitrificans, 456Bicarbonate requirement-effect on lag period, 712Blastomyces dermatitidis-monosaccharide and chitin content of cell walls, 870-yeast- and mycelial-phase cell walls of, 870Boticinogeny-in C. botulinum, 143Bovine strains of Mycobacterium-wax D from, 548

Candida utilis-fatty acid composition of, 753-temperature variation in, 753Carbohydrate mutations, pleiotropic-effect on tryptophan catabolism, 513Catabolite-repressible enzyme-in E. coli, 535-pseudouridylate synthetase, 535Cell envelope fraction-from E. coli, 664-nonheme iron in, 664Cell-free extracts-glycolytic enzymes in, 864Cell growth inhibition-effect of 2-deoxyglucose on, 121Cell wall-molecular sieving by, 718

iii

SUBJECT INDEX

Cell wall formation-effect of 2-deoxyglucose on, 121-in S. cerevisiae, 121Chitin-content in H. capsulatum and B. dermatitidis, 870Chromosome replication-of E. coli B/r, 95Citrate cycle and related metabolism-of L. monocytogenes, 770Clostridium botulinum-boticinogeny among type E and type E related or-

ganisms, 143ColB2-K, a fertility-repressed F-like sex factor, 655Colicin B-induction of, 633Competent bacteria-techniques for separating, 889Complementation of alleles, 646Conjugation in Escherichia coli K-12-effect of iododeoxuridine on, 433Cord factor-in mitochondria, 746-of C. diphtheriae, 746Corynebacterium diphtheriae-cord factor of, 746Coumarin-effect in E. coli, 599Coxiella burnetii-Embden-Meyerhof pathway in, 864ctr mutations-effect on tryptophan catabolism, 513Cytidine precursor-in M. mycoides RNA, 618Cytoplasm-phospholipids in membranes of, 918

3-Deoxy-D-arabinoheptulosonic acid-7-phosphate syn-thetase

-from E. coli, 798-purification and properties of, 798-repression in E. coli, K-12, 4062-Deoxyglucose-effect on cell wall formation, 121-relation to cell growth inhibition, 114Deoxyribonucleic acid-in E. coli, 16-transferred from UV-irradiated excision-defectiveHfr cells of E. coli, 505

Deoxyribonucleic acid breakdown-in E. coli, 599Deoxyribonucleic acid cross-links-formed after psoralen treatment, 846Deoxyribonucleic acid homologies--of P. solawacearum, 690Deoxyribonucleic acid polymerase-deficient mutant-of E. coli, 61Deoxyribonucleic acid, transferred-effect of iododeoxyuridine on, 433-in E. coli K-12, 433Deoxyribonucleic acid, transforming-bacterial inactivation of, 889Dielectric measurements in Bacillus cereus spores, 1062, 3-Dihydroxybenzoate

-conversion into enterochelin, 557-in E. coli K-12, 557Dimorphism-in M. genevensis, 114Dinitrophenyl method-determination of proteins by, 840o-Diphenoloxidase-effects of reducing agents on, 787Dipicolinic acid-reactivative action on inactive glucose dehydro-

genase, 442Division cycle-of E. coli B/r, 95

entD, entE, and entF-genes concerned with enterochelin synthesis, 557-in E. coli K-12, 557Electrolytes and water-dielectric study of, 106-in B. cereus spores, 106Electron microscopy-of staphylococcal mesosomal vesicles, 907Elementary bodies-RNA polymerase activity in, 593Embden-Meyerhof pathway-in C. burnetii, 864Endonuclease I-E. coli mutants lacking, 87Enterochelin-in E. coli K-12, 557-synthesized from 2,3-dihydroxybenzoate, 557Enzyme, phage lytic-mode of action, 697Enzyme synthesis-inhibition during limited respiration, 853Escherichia coli-L-arabinose operon in, 79-Xarog+ transductant of, 798-L-asparaginases from, 461-colicin B in, 633-3-deoxy-D-arabinoheptulosonic acid-7-phosphate

synthetase from, 798-DNA breakdown in, 599-DNA polymerase-deficient mutant of, 61-DNA synthesis in, 16-entD, entE, and entF, located in, 557-F-like sex factor from, 900--F pili, 671-genes concerned enterochelin synthesis, 557-genetic mapping of suppressor gene, 736-growth rate at elevated temperatures, 391, 397-growth rates in, 878--hexose-6-phosphate. transport system of, 74-homoserine trans-succinylase in, 397-inactivation by psoralen plus 360-nm light, 846-isoleucyl-tRNA synthetase from, 828-methionine in, 391-mRNA transcription, 610-mutants lacking endonuclease 1, 87-mutants lacking ribonuclease 1, 87-mutants lacking ribonuclease Il, 87-mutants sensitive to antibiotics, 486-mutants sensitive to synergistin A, 486

IV J. BACTERIOL.

VOL. 107, 1971 NUDJtA-J

-new suppressor in, 736-nonheme iron in, 664-operator mutants in, 8-phagelike particles, 926-phleomycin-induced death in, 599

-phospholipids of cytoplasmic membranes of, 918-pseudouridylate synthetase of, 535-ribosides in, 16-rRNA of, 543-transport mutant of, 878-tyrosine biosynthesis in, 8-UV resistance of, 16Escherichia coli B/r-chromosome replication of, 95-division cycle of, 95-ampicillin-resistant mutants of, 420Escherichia coli K-12-ColB2 factor transferred to, 900-con"ersion of 2,3-dihydroxybenzoate into entero-

chelin, 557-DNA transferred from UV-irradiated excision-de-

fective Hfr cells of, 505-lipopolysaccharide alterations affecting mating

ability, 420-lipopolysaccharide alterations affecting suscepti-

bility to sex-specific bacteriophages, 420-phoA locus in, 683-repression of 3-deoxy-D-arabinoheptulosonic acid-

7-phosphate synthetase (trp), 406-repression of tryptophan pathway enzymes, 406-temperature-sensitive recovery of a mutant of, 623-UV irradiated, 623-ColB2 factor transferred from, 900Ethionine stimulation-of porphyrin overproduction in P. denitri.ficans, 456Ethylenediaminetetraacetic acid-reactivative action on inactive glucose dehydro-

genase, 442

Fatty acid composition-effects of temperature variation on, 753-in C. utilis, 753-in M. genevensis, 114Fatty acids, unsaturated-hydrogenation by Treponema (Borrelia) strain

B25, 519Fertility-repressed mutants-from E. coli, 900Fimbriate transductants-dependent on motility, 655-of S. typhimiurium, 655F-like sex factor-ColB2, 900F pili-effects of temperature on, 671-growth of, 671D-fucose-gratuitous inducer of L-arabinose operon, 79Fructose-1, 6-diphosphatase-in yeast, 401-inactivation by glucose, 401

Genetic derepression

I INLItA V

-of isoleucine-valine enzymes, 824Genetic mapping-of suppressor gene in E. coli, 736-in B. licheniformis, 636Genetic recombination-in Micromonospora, 415Glucose-inactivation of fructose-I, 6-diphosphatase, 401Glucose dehydrogenase, inactive-obtained from heated spores of B. subtilis, 442-reactivative action of dipicolinic acid on, 442-reactivative action of EDTA on, 442Glycerol-requiring mutant

of B. subtilis, 790Glycolytic enzymes-in cell-free extracts of C. burnetil, 864Glyoxylate bypass-in L. monocytogenes, 770Growth inhibition-by isoleucine hydroxamate, 741-in S. marcescens, 741Growth rate-at elevated temperatures, 391, 397-in E. coli, 391, 397-in E. coli mutant, 878-limitation by methionine, 391-transport-limited, 878

Hexose-6-phosphate transport system-of E. coli, 74-kinetics of, 74Hfr cells-DNA transferred from, 505-of E. coli K-12, 505-UV-irradiated, excision-defective, 505Hfr Hayes strain-new suppressor carried by, 736Histoplasma capsulatum-monosaccharide and chitin content of cell walls, 870-yeast- and mycelial-phase cell walls of, 870Homoserine trans-succinylase-reversible inhibition of, 397Hydrocarbons-in M. cerificans metabolism, 806-microbial assimilation of, 806Hydrogenation of unsaturated fatty acids-by Treponema (Borrelia) strain B25, 519Hydrogenomonas eutropha-bicarbonate requirement, 712-elimination of lag period, 712Hydroxamic acids, secondary-in B. megaterium, 491-role in active transport of iron, 4912-Hydroxy-3-(cyclohexylpropyl)-1,,4-naphthoquinone-bactericidal action on B. megaterium, 528-biological and biochemical effects produced by, 528Hyperinducibility-from mutation in structural genes, 34

Immunochemical comparison of L-asparaginases, 461Iododeoxyuridine-effect on conjugation in E. coli K-12, 433-effect on transferred DNA in E. coli K-12, 433

VI TDII=Cr TXTr-%r=

SUBJECT INDEX

Iron, active transport of-in B. megateriuni, 491-role of secondary hydroxamic acids, 491Iron, nonheme-in E. coli, 664Isoleucine hydroxamate-an isoleucine antagonist, 741-growth inhibitor in S. marcescens, 741Isoleucine-valine biosynthetic enzymes-genetic derepression of, 824-in S. marcescens, 824-multivalent repression of, 824Isoleucyl-transfer ribonucleic acid synthetase-biochemical characterization of, 828-from E. coli, 828

Kinetics of exogenous induction, 74jl-Ketoadipate pathway-in A. eutrophus, 468, 476-regulation of, 476

Lag period-elimination in H. eutropha, 712Lipid composition-of Arthrobacter species, 130Lipids of Mucor genevensis, 114Lipopolysaccharide alterations-affecting mating ability in E. coli, 420-affecting susceptibility to sex-specific bacterio-

phages, 420Listeria moniocytogenes-citrate cycle of, 770-glyoxylate bypass in, 770-oxidation of pyruvate in, 770Lysin, group D-A component of, 551-of S. zymogelnes, 551Lytic agent-active against S. aureus, 499

Macromolecular synthesis-inhibition by bacteriocin, 833Mating ability in Escherichia coli-lipopolysaccharide alterations affecting, 420Mesosomal vesicles-from S. aureus, 907Mesosomes--ultrastructure during release and purification, 907Messenger ribonucleic acid-transcription of, 610Methionine-in E. coli, 391-limitation of growth rate by, 391Microbial deoxyribonucleic acids-asymmetric template function, 610Micrococcus cerificans-assimilation of hydrocarbons in, 806-phospholipid metabolism in, 806Micromonospora-genetic recombination in, 415Micromonospora chalcea-biochemical mutants of, 415Micromonospora echiniospora-biochemical mutants of, 415

Micromonzospora purpurea-biochemical mutants of, 415Minicells-plasmid containing, 21-RNA and protein synthesis in, 21Mitochondria-cord factor in, 746Mitomycin C-in colicin B induction, 633Molecular sieving-by B. megaterium, 718Monosaccharide-content in H. capsulatum and B. dermatitidis, 870Motility-dependent transductants-fimbriate, 655-of S. typhimurium, 655Mucor genevensis-anaerobic growth in, 114-fatty acid composition of, 114-dimorphism in, 114-sterol composition of, 114Multivalent repression-of isoleucine-valine enzymes, 824Mutants-of E. coli, sensitive to antibiotics, 486Mycobacterium-bovine strains of, 548-wax D from, 548Mycobacterium leprae-o-diphenoloxidase of, 787Mycoplasma mycoides var. capri-rRNA and tRNA from, 618

Neurospora crassa-end-product regulation of the tryptophan-nico-

tinic acid pathway in, 448-proteins of, 840NH2-terminal residues-of N. crassa proteins, 840Noncompetent bacteria-techniques for separating, 840Nonheme iron-in E. coli, 664Nucleosides-in tRNA of M. mycoides, 618

Operator mutants-in E. coli, 8-isolation and characterization of, 8

Penicillinase plasmids-in S. aureus, 68-stability of, 68Peptide mutations-in B. licheniijormis, 636Phenotypic characterization-of P. solainacearum, 690Pheomycin-induced death in Escherichlia coli, 599phoA locus-in E. coli, 683Phosphatidylethanolamine-deficient mutant-of B. subtilis, 704Phosphoglucoisomeraseless mutant of yeast, 759

Vi J. BACTERIOL.

SUBJECT INDEX

Phospholipid metabolism-in M. cerificans, 806-in mutant of B. subtilis, 790Phospholipids-of membranes in E. coli cytoplasm, 918Plasmid-containing minicells, 21Pleiotropic carbohydrate mutations-effect on tryptophan catabolism, 513Polyribosomes-new method for extraction, 659Porphyrin overproduction-betaine, requirement for, 456-by P. denitrificans, 456Protein synthesis-in minicells, 21Proteins-of N. crassa, 840Protoplast-molecular sieving by, 718Protoplast membranes-from S. aureus, 907Pseudomonas dentitrificans-porphyrin overproduction by, 456Pseudomonas solanacearum-DNA homologies of, 690-phenotypic characterization of, 690Pseudouridylate synthetase-a catabolite-repressible enzyme, 535-of E. coli, 535Psoralen-inactivation of E. coli and phage lambda by, 846Pyronin Y-effect in E. coli, 599Pyruvate-oxidation of, in L. monocytogenes, 770

Radiation-induced responses of Escherichia colipolAI-, 61

Reducing agents-effects on o-diphenoloxidase, 787Respiration-effects of limitation on enzyme synthesis, 853Ribonuclease I-E. coli mutants lacking, 87Ribonuclease II-E. coli mutants lacking, 87Ribonucleic acid extraction-new method, 659Ribonucleic acid polymerase activity-DNA-dependent, 593-in trachoma elementary bodies, 593Ribonucleic acid synthesis-in minicells, 21Ribonucleic acid transcription-effect of NaCl on, 593Ribosides-in E. coli, 16Ribosomal ribonucleic acid-accumulation in S. typhimurium, 134-of B. stearothermophilus, 543

of E. coli, 543Ribosomal ribonucleic acid homologies-of diverse bacterial species, 543Ribosomes

-accumulation in S. typhimurium, 134-effect of sulfhydryl reagents on, 53-of B. subtilis, 53Rumen spirochete-Treponema (Borrelia) strain B25, 519

Saccharomyces cerevisiae-adenine-requiring mutants, 1-allelic complementation in, 646-cell wall formation in, 121-glucose and fructose metabolism in mutant of, 759-inactivation of fructose-i, 6-diphosphatase in, 401-mutant types at ade3 locus, 1-phosphoglucoisomeraseless mutant of, 759Salmonella typhlimurium-fimbriate transductants, 655-recovery from thermal injury, 134-ribosomal RNA accumulation in vivo, 134-ribosome accumulation in vivo, 134Schizosaccharomyces pombe-new method for extraction of polyribosomes, 659-new method for extraction of RNA, 659Secondary hydroxamic acids-in B. megaterium, 491-role in active transport of iron, 491Self-catabolite repression--in the ara operon, 840Serratia marcescens-L-asparaginases from, 461-bacteriocin JF 246 from, 833-isoleucine antagonization in, 741-isoleucine hydroxamate in, 741-isoleucine-valine biosynthetic enzymes in, 824Sex-specific bacteriophages-lipopolysaccharide alterations affecting, 420Sporulation-in B. subtilis, 815-protease activities during, 815Staphylococcal bacteriophage-associated lysin, 499Staphtylococcus aureus-lytic agent active against, 499-mesosomal vesicles and protoplast membranes

from, 907-penicillinase plasmids in, 68-staphylococcal bacteriophage-associated lysin, 499Sterol composition-in M. genevensis, 114Streptococcus zymogenes-A component of group D lysin produced by, 551Suppressor, extragenic-carried by Hfr Hayes strain, 736-in E. coli, 736Synergistin A-mutants of E. coli sensitive to, 486Synthesis-of enzymes under limited respiration, 853

Temperature-effects on F pili growth, 671Temperature variation-effects on fatty acid composition, 753Thermal injury-recovery of S. typhimurium from, 134

VOL. 107, 1971 *vi

SUBJECT INDEX

Thermophilic phage lytic enzyme-action on B. stearothermophilus cell walls, 697Thiopurines-effect in E. coli, 599Trachoma elementary bodies-RNA polymerase activity in, 593Transductants-fimbriate, 655-of S. typhimurium, 655Transfer ribonucleic acid-modified nucleosides in, 618Transferred deoxyribonucleic acid-effect of iododeoxyuridine on, 433-in E. coli K-12, 433Transformation-competence for, in B. licheniformis, 636Transforming deoxyribonucleic acid-bacterial inactivation of, 889Transport mutant-of E. coli, 879Treponema (Borrelia) strain B25-a rumen spirochete, 519-hydrogenation of unsaturated fatty acids by, 519Tryptophan catabolism--effect of pleiotropic carbohydrate mutations on, 513Tryptophan-nicotinic acid pathway-end-product regulation of, 448-in N. crassa, 448Tryptophan pathway enzymes

Month

JulyAugust

September

-repression in E. coli K-12, 406Tyrosine biosynthesis-in E. coli, 8

Ultraviolet-induced responses of Escherichia colimutant, 61

Ultraviolet recovery-of E. coli mutant, 623Ultraviolet resistance enhancement-in E. coli, 16Unsaturated fatty acids-hydrogenation by Treponema (Borrelia) strain B25,

519Uric acid-aerobic metabolization by B. fastidiosus, 780Uridine precursor-in M. mycoides RNA, 618

Virus-induced membranes-in E. coli cytoplasm, 918

Wax D-from different bovine strains of Mycobacterium, 548

X-ray-induced responses of Escherichia coli mutant, 61

Yeast-inactivation of fructose-i, 6-diphosphatase in, 401-phosphoglucoisomeraseless mutant of, 759

INDEX TO DATE OF ISSUE

Date of Issue

9 July 197112 August 1971

8 September 1971

Pages

1-39039 1-592593-928

*iii J. BACTERIOL.

JOURNALOF

BACTERIOLOGY

VOLUME 107

BALTIMORE, MD. 21202

1971

JOURNAL OF BACTERIOLOGYVOLUME 107

EDITORIAL BOARD

L. LEON CAMPBELL, Editor-in-Chief (1975)University ofIllinois, Urbana

R. L. ANDERSON, Editor (1975)Michigan State University, East Lansing

S. G. BRADLEY, Editor (1974)Virginia Commonwealth University, Richmond

ROY CURTISS III, Editor (1974)The University of Tennessee, Oak Ridge

ALLEN G. MARR, Editor (1973)University of California, Davis

Martin Alexander (1972)W. Lane Barksdale (1972)M. E. Bayer (1972)Claire M. Berg (1973)Robert W. Bernlohr (1973)Allan Campbell (1972)A. J. Clark (1972)Royston C. Clowes (1972)V. W. Cochrane (1972)Stanley N. Cohen (1973)S. F. Conti (1972)A. L. Demain (1972)John A. DeMoss (1972)Walter J. Dobrogosz (1973)Martin Dworkin (1973)Alan D. Elbein (1973)Herman Friedman (1972)A. T. Ganesan (1972)Howard Goldflne (1972)Joseph S. Gots (1972)Harlyn 0. Halvorson (1972)F. M. Harold (1973)

Leonard Hayflick (1972)George Hegeman (1971)Charles E. Helmstetter (1972)Joseph T. Holden (1972)John G. Holt (1972)Stanley C. Holt (1971)John Ingraham (1973)John H. Law (1971)Hillel S. Levinson (1972)W. R. Lockhart (1972)Donald G. Lundgren (1971)Manley Mandel (1972)Paul Margolin (1972)Alvin Markovitz (1973)Julius Marmur (1971)Robert G. Martin (1971)M. L. Morse (1972)Richard P. Novick (1973)Leo Parks (1973)H. J. Phaff (1972)Allen T. Phillips (1973)Jack Preiss (1971)Charles C. Remsen III (1973)

William S. Reznikoff (1973)H. V. Rickenberg (1973)Antonio H. Romano (1973)John Roth (1973)M. R. J. Salton (1972)Moselio Schaechter (1973)David Schlessinger (1971)Simon Silver (1973)W. R. Sistrom (1972)Lucille Smith (1972)Bernard S. Strauss (1972)Noboru Sueoka (1972)Paul S. Sypherd (1971)A. L. Taylor (1971)H. E. Umbarger (1973)Arthur Weissbach (1971)N. E. Welker (1973)R. W. Wheat (1972)Helen R. Whiteley (1972)Meyer J. Wolin (1973)Barbara E. Wright (1971)Frank E. Young (1973)Stanley A. Zahler (1971)

ROBERT A. DAY, Managing Editor, 4715 Cordell Ave.,

EX OFFICIOMORRIS F. SHAFFER, President (1971-1972)

DONALD E. SHAY, Secretary

The Journal of Bacteriology, a publication of the American Societyfor Microbiology, 4715 Cordell Ave., Bethesda, Md. 20014, is devoted to

the advancement and dissemination of fundamental knowledge concerningbacteria and other microorganisms. The Journal is published monthly, andthe twelve numbers are divided into four volumes per year. The subscrip-tion price is S52 (Postal Union Countries, S54; other foreign, S55) per

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changes of address, incorrect journals, etc., should be directed to theAmerican Society for Microbiology, 1913 1 St., N.W., Washington, D.C.20006. Nonmembers of the Society should write to The Williams & Wil-kins Co., 428 E. Preston St., Baltimore, Md. 21202, regarding institutionof nonmember, subscriptions and nonreceipt of journals. Publishedmonthly by the ASM at 428 E. Preston St., Baltimore, Md. 21202.

Second class postage paid at Baltimore, Md. 21202, and at additional

mailing offices.Made in the United States of America.

Copyright@ 1971, American Society for MicrobiologyAll Rights Reserved.

Bethesda, Md. 20014

Volume 107 Contents for July Number 1Taxonomy and Ecology

Differentiation of the Ribosomal Protein Compositions in the Genus Escherichiaand Its Related Bacteria. SYOZO OSAWA, TAKUZI ITOH, AND EIKO OTAKA..... 168

Taxonomy of Marine Bacteria: the Genus Beneckea. PAUL BAUMANN, LINDA BAU-MANN, AND M. MANDEL................................................ 268

Structure and Arrangement of Flagella in Species of the Genus Beneckea and Photo-bacterium fischeri. RICHARD D. ALLEN AND PAUL BAUMANN................. 295

Microbial Life at 90 C: the Sulfur Bacteria of Boulder Spring. T. D. BROCK, M. L.BROCK, T. L. BOTT, AND M. R. EDWARDS.................................. 303

Base Composition of Deoxyribonucleic Acid Isolated from Athiorhodaceae. MAR-VIN SILVER, SOLOMON FRIEDMAN, ROGER GUAY, JAQUE COUTURE, AND ROBERTTANGUAY.............................. 368

Deoxyribonucleic Acid Hybridization Among Strains of Lactobacilli. JOSEPHINESIMONDS, P. ARNE HANSEN, AND SITARAMA LAKSHMANAN..................... 382

Morphology and UltrastructureNature and Origins of Phosphorus Compounds in Isolated Cell Walls of Staphylo-

coccus aureus. DAVID MIRELMAN, D. R. D. SHAW, AND JAMES T. PARK....... 239Giant Cysts and Cysts with Multiple Central Bodies in Azotobacter vinelandii. G. D.

CAGLE AND G. R. VELA.................................................. 315Paracrystalline Sheets Reaggregated from Solubilized Exosporium of Bacillus cereus.

T. CABRERA BEAMAN, H. STUART PANKRATZ, AND PHILIPP GERHARDT.......... 320Ultrastructure of the Cell Wall of Erwinia amylovora. PI-Yu HUANG AND ROBERT

N. GOODMAN........................................................... 361

Genetics and Molecular BiologyNew Mutant Types at the ade3 Locus of Saccharomyces cerevisiae. CHRISTOPHER

A. LOMAX, TREVOR S. GROSS, AND ROBIN A. WOODS........................ 1Regulation of Tyrosine Biosynthesis in Escherichia coli K-12: Isolation and Charac-

terization of Operator Mutants. INA E. MATTERN AND J. PITTARD........... 8Influence of Ribosides on Ultraviolet Resistance of Escherichia coli: Role of Deoxy-

ribonucleic Acid Synthesis in the Ultraviolet Resistance Enlhancement AfterAmino Acid Prestarvation. MILENA SEDLIAKOVA, EVA STEFUNKOVA, AND Lu-BICABERNAETOVA....... 16

Synthesis of Ribonucleic Acid and Protein in Plasmid-Containing Minicells of Esch-ericha coli K-12. KENNETH J. ROOZEN, RAYMOND G. FENWICK, JR., AND RoYCURTISS III............................................................. 21

Hyperinducibility as a Recult of Mutation in Structural Genes and Self-CataboliteRepression in the ara Operon. LEONARD KATZ AND ELLIS ENGLESBERG.34

Effect of Sulfhydryl Reagents on the Ribosomes of Bacillus subtilis. RAJINDER SINGHRANU AND AKIRA KAJI................................................... 53

Ultraviolet- and X-Ray-Induced Responses of a Deoxyribonucleic Acid Polynierase-Deficient Mutant of Escherichia coli. M. C. PATERSON, J. M. BOYLE,AND R. B.SETLOW.................................................................. 61

Stability of Penicillinase Plasmids in Staphylococcus aureus. L. H. JOHNSTON ANDK. G. H. DYKE.......................................................... 68

Kinetics of Exogenous Induction of the Hexose-6-Phosphate Transport System ofEscherichia coli. HERBERT H. WINKLER ....................................74

D-Fucose as a Gratuitous Inducer of the L-Arabinose Operon in Strains of Escherichiacoli B/r Mutant in Gene araC. SUSAN BEVERIN, DAVID E. SHEPPARD, AND SANGSHIN PARK............................................................. 79

Mutants of Escherichia coli Lacking Endonuclease I, Ribonuclease I, or RibonucleaseII. MICHEL WRIGHT...................................................... 87

Chromosome Replication and the Division Cycle of Escherichia coli B/r. H. E.KUBITSCHEK AND M. L. FREEDMAN.........................................95

Precursor Ribosomal Ribonucleic Acid and Ribosome Accumulation In Vivo Duringxi

xii CONTENTS

the Recovery of Salmonella typhimurium from Thermal Injury. RICHARD I1.ToMLINS AND Z. JOHN ORDAL......................134

Polynucleotide Sequence Relationships Among Some Bacterial Plasmids. PATRICIAGUERRY AND STANLEY FALKOW.......................372

Petite Mutation in Yeast. II. Isolation of Mutants Containing Mitochondrial Deoxy-ribonucleic Acid of Reduced Size. ELIZABETH S. GOLDRING, LAWRENCE I.GROSSMAN, AND JULIUS MARMUR......................377

Isolation and Genetic Analysis of a Strain of Eselherichia coli K- 12 with an AmberrecA Mutation. DAVID W. MOUNT.....................388

Physiology and MetabolismRegulation of the Pathway fol the Degradation of Anthranilate in Aspergillus niger.

P. V. SUBBA RAO, N. S. SREELEELA, R. PREMAKUMAR, AND C. S. VAIDYANATHAN. 100Dielectric Study of the Physical State of Electrolytes and Water Within Bacillus

cereus Spores. EDWIN L. CARSTENSEN, ROBERT E. MARQUIS, AND PHILIPPGERHARDT................................106

Fatty Acid and Sterol Composition of Mucor genevensis in Relation to Dimorphismand Anaerobic Growth. P. A. GORDON, P. R. STEWART, AND G. D. CLARK-WALKER.................................114Effect of 2-Deoxyglucose on Cell Wall Formation in Saccharom,yces cerevisiae andIts Relation to Cell Growth Inhibition. P. BIELY, Z. KRA'TKY, J. KOVA'RIK, AND9.BAUER.................................121

Lipid Composition of Some Species of Arthrobacter. N. SHAW AND D. STEAD....130Boticinogeny and Actions of the Bacteriocin. KATHRYN L. ANASTASIO, J. A. Sou-

CHECK, AND H. SUGIYAMA.........................143Occurrence of Pyruvic Acid in Capsular Polysaccharides from Various Klebsiella

Species. B. J. GORMUS, ROBERT W. WHEAT, AND JOHN F. PORTER........150Enzymes of Carbohydrate Metabolism in Thiobacillus Species. ABDUL MATIN AND

SYDNIEY C. RITTENBERG..........................179Physiological Studies of Methane and Methanol-Oxidizing Bacteria: Oxidation of

C- 1 Compounds by Methylococcus capsulatus. RAMESH N. PATEL AND DEREKS. HOARE.................................187

Regulation of Tryptophan Biosynthetic Enzymes in Neurospora crassa. GABRIELLESTER..................................193

Characterization of the Late Steps of Microbial Heme Synthesis: Conversion ofCoproporphyrinogen to Protoporphyrin. N. J. JACOBS, J. M. JACOBS, AND P.BRENT..................................203

Effect of Nutrient Concentration on the Growth of Escherichia coli. TALAAT E.SHEHATA AND ALLEN G. MARR.......................210

Partial Purification and Properties of a Highly Specific Trehalose Phosphate Phos-phatase from Mycobacterium smegmatis. MIKE MATULA, MIKE MITCHELL,AND ALAN D. ELBEIN...........................217

Protein A Mutants of Staphylococcus aureus. ARNE FORSGREN, KRISTINA NORD-STROM, LENNARr PHILIPSON, AND JOHN SJdQUIST................245

Effect of Shift-Down and Growth Inhibition on Phospholipid Metabolism of Esch-erichia coli. J. P. G. BALLESTA AND M. SCHAECHTER.............251

Metabolism of T4 Bacteriophage Ghost-Infected Cells: Effect of Bacteriophage andGhosts on the Uptake of Carbohydrates in Escherichia coli B. HERBERT H.WINKLER AND DONNA HARDY DUCKWORTH..................259

Interactions of Alkaline Phosphatase and the Cell Wall of Pseudomonas aeruginosa.K.-J. CHENG, J. M. INGRAM, AND J. W. COSTERTON..............325

Spore Refractility in Variants of Bacillus cereus Treated with Actinomycin D. Su-SANNE M. PEARCE AND P. C. FITZ-JAMES...................337

Comparison of the Biochemistry and Rates of Synthesis of Mesosomal and Periph-eral Membranes in Bacillus subtilis. CEPHAs T. PATCH AND OTTo E. LANDMAN.. 345

Ribosomal Helices: Formation in Escherichia coli During Acidic Growth. ROBERTK. NAUMAN, DAVID J. SILVERMAN, AND HERBERT VOELZ............358

CONTENTS

Mating Responses in Candida lipolytica. A. I. HERMAN........................ 371Variation in the Proportion of 50S and 30S Ribosomal Subunits at Different Growth

Rates. GEORGE A. MICHAELS............................................. 385

EnzymologyProperties of a Salmonella typhimurium Mutant with an Incomplete Deficiency of

Uridinediphosphogalactose-4-Epimerase. V. KRISHNAPILLAI, D. G. MACPHEE,AND B. A. D. STOCKER................................................... 155

Properties of an Extracellular f3-Galactosidase Secreted by Neurospora crassa. PHILIPC. COMP AND GABRIEL LESTER............................................ 162

Glucosylation of Teichoic Acid: Solubilization and Partial Characterization of theUridine Diphosphoglucose: Polyglycerolteichoic Acid Glucosyl Transferasefrom Membranes of Bacillus subtilis. DAVID BROOKS, LAURA L. MAYS, Y.HATEFI, AND FRANK E. YOUNG........................................... 223

Characterization of the Membrane-Bound Succinic Dehydrogenase of Micrococcuslysodeikticus. JERRY J. POLLOCK, REGINA LINDER, AND MILTON R. J. SALTON..... 230

Apparent Lack of Control by Repression of Arginine Metabolism in Blue-Green Algae.W. HOOD AND N. G. CARR............................. .... 365

Observations on the Rhodanese Activity of Desulfotomaculum nigrificans. CAROLYNP. BURTON AND J. M. AKAGI..................................... ......... 375

Volume 107 Contents for August Number 2

Taxonomy and EcologyHomology of Ribosomal Ribonucleic Acid of Diverse Bacterial Species with

Escherichia coli and Bacillus stearothermophilus. B. PACE AND L. LEON CAMPBELL. 543Comparison of the Morphology and Deoxyribonucleic Acid Composition of 27 Strains

of Nitrifying Bacteria. STANLEY W. WATSON AND MANLEY MANDEL............. 563

Genetics and Molecular BiologyRepression of 3-Deoxy-D-Arabinoheptulosonic Acid-7-Phosphate Synthetase (trp) and

Enzymes of the Tryptophan Pathway in Escherichia coli K-12. J. CAMAKARISAND J. PITTARD............................................... ........ 406

Genetic Recombination in Micromonospora. MARIA BERETTA, MIRELLA BETTI, ANDMARIO POLSINELLI........................................................ 415

Ampicillin-Resistant Mutants of Escherichia coli K-12 with Lipopolysaccharide Altera-tions Affecting Mating Ability and Susceptibility to Sex-Specific Bacteriophages.DAVID A. MONNER, STAFFAN JONSSON, AND HANS G. BOMAN................... 420

Effect of Iododeoxyuridine upon Conjugation and the Fate of Transferred Deoxyribo-nucleic Acid in Escherichia coli K-12. ALAN D. COOPER, MURRIE W. BURGAN,CATHERINE W. WHITE, AND ROBERT L. HERRMANN.......................... 433

Deoxyribonucleic Acid Transferred from Ultraviolet-Irradiated Excision-DefectiveHfr Cells of Escherichia coli K-12. B. M. WILKINS, S. E. HOLLOM, AND W. D.RUPP ...................................................... ........... 505

Effect of Pleiotropic Carbohydrate Mutations (ctr) on Tryptophan Catabolism. ROLFDAHL, RICHARD J. WANG, AND M. L. MORSE................... ............. 513

Location of Three Genes Concerned with the Conversion of 2, 3-Dihydroxybenzoateinto Enterochelin in Escherichia coli K- 12. R. K. J. LUKE AND F. GIBSON........ 557

Enriched Selection of Dominant Mutations: Histidine Operator Mutations. GEORGEW. CHANG, DANIEL STRAUS, AND BRUCE N. AMES............................ 578

Identification of the Forms of Vitamin B6 Present in the Culture Media of "VitaminB6 Control" Mutants. WALTER B. DEMPSEY AND LARRY J. ARCEMENT.......... 580

Observations on Replication and Cell Division in Synchronous Cultures of the Blue-Green Alga Anacystis nidulans. M. HERDMAN AND N. G. CARR.583

.x.ii

CONTENTS

Ribonucleic Acid Synthesis in Bacteria Treated with Toluene. R. L. PETERSON, C. W.RADCLIFFE, AND N. R. PACE............................................... 585

Inhibition of Arginyl-Transfer Ribonucleic Acid Synthetase Activity of Escherichiacoli by Arginine Biosynthetic Precursors. D. W. YEM AND L. S. WILLIAMS........ 589

Physiology and MetabolismReactivative Action of Ethylenediaminetetraacetic Acid or Dipicolinic Acid on Inactive

Glucose Dehydrogenase Obtained from Heated Spores of Bacillus subtilis. Y.HACHISUKA AND K. TOCHIKUBO.................. ........................ 442

End-Product Regulation of the Tryptophan-Nicotinic Acid Pathway in Neurosporacrassa. GABRIEL LESTER .................................................. 448

Porphyrin Overproduction by Pseudomonas denitrificans: Essentiality of Betaine andStimulation by Ethionine. ARNOLD L. DEMAIN AND RAYMOND F. WHITE....... 456

Mutants of Escherichia coli Sensitive to Antibiotics. HERBERT L. ENNIS.............. 486Active Transport of Iron in Bacillus megaterium: Role of Secondary Hydroxamic

Acids. W. B. DAVIS AND B. R. BYERS...................................... 491Hydrogenation of Unsaturated Fatty Acids by Treponema (Borrelia) Strain B25, a Ru-

men Spirochete. M. T. YOKOYAMA AND C. L. DAVIS......................... 519Bactericidal Action of 2-Hydroxy-3-(Cyclohexylpropyl)-1,4-Naphthoquinone on

Bacillus megaterium. JOHN G. OLENICK, THOMAS M. COOK, AND FRED E. HAHN... 528Wax D from Different Bovine Strains of Mycobacterium. D. MIGLIORE, J. AUGIER,

H. BOISVERT, AND P. JOLLES............................................... 5482-Keto-3-Deoxygluconate, an Intermediate in the Fermentation of Gluconate by

Clostridia. R. BENDER, J. R. ANDREESEN, AND G. GOTTSCHALK................ 570Folate Replacement by Tetrahydrohomopteroate in Triazine-Resistant Bacteria.

CLARA S. GENTHER, JOHN H. ROSZMANN, CARL C. SMITH, AND RoY L. KISLIUK ... 576

Enzymology

Growth Rate of Escherichia coli at Elevated Temperatures: Limitation by Methionine.ELIORA Z. RON AND BERNARD D. DAVIS.............................. .........391

Growth Rate of Escherichia coli at Elevated Temperatures: Reversible Inhibition ofHomoserine Trans-Succinylase. ELIORA Z. RON AND M. SHANI................ 397

Inactivation of Fructose-i,6-Diphosphatase by Glucose in Yeast. CARLOS GANCEDO. . 401Immunochemical Comparison of L-Asparaginases from Serratia marcescens and

Escherichia coli. ARTHUR W. PHILLIPS, JOHN W. BOYD, DONALD A. FERGUSON,JR., AND A. ALVIN MARUCCI.............................................. 461

Dissimilation of Aromatic Compounds by Alcaligenes eutrophus. B. F. JOHNSONAND R. Y. STANIER....................................................... 468

Regulation of the ,B-Ketoadipate Pathway in Alcaligenes eutrophus. B. F. JOHNSONAND R. Y. STANIER....................................................... 476

Staphylococcal Bacteriophage-Associated Lysin: a Lytic Agent Active Against Staphylo-coccus aureus. STEPHEN A. SONSTEIN, JAY M. HAMMEL, AND AMEDEO BONDI ...... 499

Pseudouridylate Synthetase of Escherichia coli: Catabolite-Repressible Enzyme. L. R.SOLOMON AND T. R. BREITMAN............................................. 535

Characterization of the A Component of Streptococcus zymogenles Lysin. PAUL A.GRANATO AND ROBERT W. JACKSON........................................ 551

Complementary Functioning of Nitrogenase Components from a Blue-Green Algaand a Photosynthetic Bacterium. R. V. SMITH, A. TELFER, AND M. C. W. EVANS. 574

Volume 107 Contents for September Number 3Taxonomy and Ecology

Phenotypic Characterization and Deoxyribonucleic Acid Homologies of Pseudomonassolanacearum. N. J. PALLERONI AND M. DOUDOROFF.......................... 690

XiV

CONTENTS

Morphology and UltrastructureSelection of Fimbriate Transductants of Salmonella typhimurium Dependent on Mo-

tility. D. C. OLD AND J. P. DUGUID............... 655Monosaccharide and Chitin Content of Cell Walls of Histoplasma capsulatum and

Blastomyces dermatitidis. JUDITH E. DOMER................................ 870Electron Microscopy During Release and Purification of Mesosomal Vesicles and

Protoplast Membranes from Staphylococcus aureus. TERRY J. POPKIN, THEODORES. THEODORE, AND ROGER M. COLE....................................... 907

Genetics and Molecular BiologyDeoxyribonucleic Acid-Dependent Ribonucleic Acid Polymerase Activity in Purified

Trachorna Elementary Bodies: Effect of Sodium Chloride on Ribonucleic AcidTranscription. ISRAEL SAROV AND YECHIEL BECKER.......................... 593

Effects of Coumarin, Thiopurines, and Pyronin Y on Amplification of Phleomycin-Induced Death and Deoxyribonucleic Acid Breakdown in Escherichia coli. G. W.GRIGG, MERILYN J. EDWARDS, AND D. J. BROWN.................. 599

Asymmetric Template Function of Microbial Deoxyribonucleic Acids: Transcriptionof Messenger Ribonucleic Acid. LOLA MARGULIES, VIRGILIJA REMEZA, ANDRIVKA RUDNER..... 610

Biosynthetic Precursors of Some Modified Nucleosides in the Transfer RibonucleicAcid of Mycoplasma mycoides var. capri. R. T. WALKER..................... 618

Temperature-Sensitive Recovery of a Mutant of Escherichia coli K-12 Irradiated withUltraviolet Light. Y. SHIMAZU, M. MORIMYO, AND K. SUZUKI................. 623

Mitomycin C and Temperature Induction of Colicin B in the Absence of Deoxy-ribonucleic Acid Synthesis. CELMA HAUSMANN AND ROYSTON C. CLOWES........ 633

Genetic Mapping of Genes Concerned with Glutamyl Polypeptide Production byBacillus licheniformis and a Study of Their Relationship to the Development ofCompetence for Transformation. R. W. MCCUEN AND C. B. THORNE ....... 636

Allelic Variability in Comparative Complementation Confirming that the adel2-Speci-fied Protein of Yeast is Bifunctional. BEN-ZION DORFMAN..................... 646

New Method for Extraction of Ribonucleic Acid and Polyribosomes from Schizo-saccharomyces pombe. H. C. BIRNBOIM..................................... 659

Some Effects of Temperature on the Growth of F Pili. CHARLES P. NOVOTNY ANDKAREN LAVIN.... ............................................... 671

Location and Orientation of the phoA Locuis on the Escherichia coli K-12 LinkageMap. ATSuo NAKATA, GEORGE R. PETERSON, E. LUCILLE BROOKS, AND FRANK G.ROTHMAN.............................................................. 683

New Suppressor in Escherichia coli. R. R. B. RUSSELL AND A. J. PITTARD............ 736NH2-Terminal Residues of Neurospora crassa Proteins. HYUNE Mo RHO AND A. GIB

DEBUSK ................................I ................................. 840Inactivation of Escherichia coli, F' Episomes at Transfer, and Bacteriophage Lambda

Systems by Psoralen Plus 360-nm Light: Significance of Deoxyribonucleic AcidCross-Links. RONALD S. COLE............................................ 846

Transport-Limited Growth Rates in a Mutant of Escherichia coli. KASPER VONMEYENBURG............................................................ 878

Bacterial Inactivation of Transforming Deoxyribonucleate. FLORENCE P. HASELTINEAND MAURICE S. Fox.................................................. 889

ColB2-K 77; a Fertility-Repressed F-Like Sex Factor. CELMA HAUSMANN ANDROYSTON C. CLOWES .................................................... 900

Presence of Bacteriophage-Like Inhibitory Particles in Escherichia coli. J. N. REEVE,R. A. WEIGAND, AND J. W. GREENAWALT................................... 926

Physiology and MetabolismProperties of Nonheme Iron in a Cell Envelope Fraction from Escherichia coli. I. C.

KIM AND P. D. BRAGG................................................. 664Structure of the Cell Wall of Bacillus stearothermophilus: Mode of Action of a Thermo-

philic Bacteriophage Lytic Enzyme. N. E. WELKER.......................... 697

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CONTENTS

Isolation and Characterization of a Phosphatidylethanolamine-Deficient Mutant ofBacillus subtilis. JAMES L. BEEBE ... ......... 704

Bicarbonate Requirement for Elimination of the Lag Period of Hydrogenomonaseutropha. RoY REPASKE, CAROL A. AMBROSE, ANNE C. REPASKE, AND MARGARETL. DE LACY............................................................ 712

Molecular Sieving by the Bacillus megaterium Cell Wall and Protoplast. RENESCHERRER AND PHILIPP GERHARDT ........................................ 718

Isoleucine Hydroxamate, an Isoleucine Antagonist. MASAHIKO KISUMI, SABUROKOMATSUBARA, MASAKI SUGIURA, AND ICHIRO CHIBATA...................... 741

Site of Action of the Cord Factor of Corynebacterium diphtheriae in Mitochondria.MASAHIKO KATO........................................................ 746

Effects of Temperature Variation on the Fatty Acid Composition of Candida utilis. I.MCMURROUGH AND A. H. ROSE......................................... 753

Glucose and Fructose Metabolism in a Phosphoglucoisomeraseless Mutant ofSaccharomyces cerevisiae. P. K. MAITRA................................... 759

Citrate Cycle and Related Metabolism of Listeria monocytogenes. TERRENCE L.TRIVETT AND ERNEST A. MEYER......................................... 770

Studies on the Physiology of Bacillus fastidiosus. H. KALTWASSER ..... ............. 780Unusual Effects of Reducing Agents on o-Diphenoloxidase of Mycobacterium leprae.

KOCHUKUNJU PRABHAKARAN ................... .......................... 787Phospholipid Metabolism in the Absence of Net Phospholipid Synthesis in a Glycerol-

Requiring Mutant of Bacillus subtilis. THOMAS T. LILLICH AND DAVID C. WHITE. 790Microbial Assimilation of Hydrocarbons: Phospholipid Metabolism. R. A. MAKULA

AND W. R. FINNERTY.............................................. .... 806Protease Activities During the Course of Sporulation in Bacillus subtilis. LouIsE

PRESTIDGE, VIVIAN GAGE, AND JOHN SPIZIZEN.............................. 815Mode of Action of a Bacteriocin from Serratia marcescens. JOHN FOULDS........... 833Phospholipids of Virus-Induced Membranes in Cytoplasm of Escherichia coli.

YOSHINARI OHNISHI ..................................................... 918

EnzymologyPurification and Properties of 3-Deoxy-D-Arabinoheptulosonic Acid-7-Phosphate

Synthetase (phe) from a XaroG+Transductant of Escherichia coli. R. J. SIMPSON,B. E. DAVIDSON, T. A. A. DOPHEIDE, S. ANDREWS, AND J. PITTARD............ 798

Multivalent Repression and Genetic Derepression of Isoleucine-Valine BiosyntheticEnzymes in Serratia marcescens. MASAHIKO KISUMI, SABURO KOMATSUBARA, ANDICHIRO CHIBATA........................................................ 824

Biochemical Characterization of a Mutant Isoleucyl-Transfer Ribonucleic Acid Syn-thetase from Escherichia coli K- 12. GERDA TREIBER AND MAURIZIO IACCARINO. 828

Selective Inhibition of Enzyme Synthesis Under Conditions of Respiratory Inhibition.RICHARD B. FLAVELL AND Dow 0. WOODWARD............................. 853

Biochemistry of Coxiella burnetii: Embden-Meyerhof Pathway. THOMAS L. MCDONALDAND L. MALLAVIA...................................................... 864

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