380 ISA was obtained by comparisons with ELISA and IFA for 66 field samples. IFA detected antibodies in 25 of the samples, while CELISA and ELISA identified 31 as positives. A closer analysis revealed possibility of false-positives in the ELISA: serum from one horse was apparently false- positive by CELISA. Although ELISA and CELISA were equally efficient, marked disparity in distinctions between positive and negative samples made CELISA highly efficient. High sensitivity and specificity, coupled with simplicity, make this test versatile and amenable to be used as a stall-side test. This assay can be used for efficient diagnosis, pre-season screening, and monitoring the antibody levels following vaccination. REFERENCES Dutta, S.K., Rice, R.M., Hughes, T.D., Savage, P.K. and Myrup, A.C., 1987. Detection of serum antibodies against Ehrlichia risticii in Potomac horse fever by enzyme-linked immunosorbent assay. Vet. Immunol. Immunopathol., 14: 85-92. Pretzman, C.I., Rikihisa, Y., Ralph, D., Gordon, J.C. and Bech-Nielsen, S., 1987. Enzyme-linked immunosorbent assay for Potomac horse fever disease. J. Clin. Microbiol., 25:31-36. Ristic, M., Holland, C.J., Dawson, J,E., Sessions, J. and Palmer, J.E., 1986. Diagnosis of equine monocytic ehrlichiosis (syn. Potomac horse fever) by indirect immunofluorescence. J. Am. Vet. Med. Assoc., 189: 39-46. Shankarappa, B. and Dutta, S.K., 1988. Production and characterization of monoclonal antibodies to Ehrlichia risticii. Am. J. Vet. Res. (in press). Shankarappa, B., Dutta, S.K., Sanusi, J. and Mattingly, B., 1989. Monoclonal antibody-mediated immunodiagnostic competitive enzyme-linked immunosorbent assay for equine monocytic ehrlichiosis. J. Clin. Microbiol., 27: 24-28. FURTHER ANNOUNCEMENTS 14th INTERNATIONAL MEETING OF ALLERGOLOGY AND CLINICAL IMMUNOLOGY OF GAILL Dates: 5-7 October 1989 Place: Lisbon, Portugal Info.: GAILL Secretariat, Rua Sampaio e Pina, 16-40, Lisbon, Portugal. IUIS-WHO ADVANCED IMMUNOLOGY COURSE, WITH FOCUS ON THE IMMUNOL- OGY OF INFECTIOUS DISEASES Dates: 14-25 November 1989 Place: New Delhi, India In[o.: Dr. F. Celada, Department of Rheumatology, Room 1410, Hospital for Joint Diseases, 301 East 17th Street, New York, NY 10003, U.S.A.

IUIS-WHO advanced immunology course, with focus on the immunology of infectious diseases

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Page 1: IUIS-WHO advanced immunology course, with focus on the immunology of infectious diseases

380

ISA was obtained by comparisons with ELISA and IFA for 66 field samples. IFA detected antibodies in 25 of the samples, while CELISA and ELISA identified 31 as positives. A closer analysis revealed possibility of false-positives in the ELISA: serum from one horse was apparently false- positive by CELISA. Although ELISA and CELISA were equally efficient, marked disparity in distinctions between positive and negative samples made CELISA highly efficient.

High sensitivity and specificity, coupled with simplicity, make this test versatile and amenable to be used as a stall-side test. This assay can be used for efficient diagnosis, pre-season screening, and monitoring the antibody levels following vaccination.

REFERENCES

Dutta, S.K., Rice, R.M., Hughes, T.D., Savage, P.K. and Myrup, A.C., 1987. Detection of serum antibodies against Ehrlichia risticii in Potomac horse fever by enzyme-linked immunosorbent assay. Vet. Immunol. Immunopathol., 14: 85-92.

Pretzman, C.I., Rikihisa, Y., Ralph, D., Gordon, J.C. and Bech-Nielsen, S., 1987. Enzyme-linked immunosorbent assay for Potomac horse fever disease. J. Clin. Microbiol., 25:31-36.

Ristic, M., Holland, C.J., Dawson, J,E., Sessions, J. and Palmer, J.E., 1986. Diagnosis of equine monocytic ehrlichiosis (syn. Potomac horse fever) by indirect immunofluorescence. J. Am. Vet. Med. Assoc., 189: 39-46.

Shankarappa, B. and Dutta, S.K., 1988. Production and characterization of monoclonal antibodies to Ehrlichia risticii. Am. J. Vet. Res. (in press).

Shankarappa, B., Dutta, S.K., Sanusi, J. and Mattingly, B., 1989. Monoclonal antibody-mediated immunodiagnostic competitive enzyme-linked immunosorbent assay for equine monocytic ehrlichiosis. J. Clin. Microbiol., 27: 24-28.

FURTHER ANNOUNCEMENTS

14th INTERNATIONAL MEETING OF ALLERGOLOGY AND CLINICAL IMMUNOLOGY

OF GAILL

Dates: 5-7 October 1989 Place: Lisbon, Portugal Info.: GAILL Secretariat, Rua Sampaio e Pina, 16-40, Lisbon, Portugal.

IUIS-WHO ADVANCED IMMUNOLOGY COURSE, WITH FOCUS ON THE IMMUNOL-

OGY OF INFECTIOUS DISEASES

Dates: 14-25 November 1989 Place: New Delhi, India In[o.: Dr. F. Celada, Department of Rheumatology, Room 1410, Hospital for Joint Diseases, 301

East 17th Street, New York, NY 10003, U.S.A.