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IS Sf Insect

Serum-free, Animal Component-free Medium for Protein Expression and Viral Vector Production Using Baculovirus Expression Systems in Sf9 and Sf21 Cells

IS Sf Insect Achieves Robust Growth in Comparison to Other Commercially-available Serum-free Insect Cell Media

IS Sf Insect delivers reliable growth of Sf9 and Sf21 insect cells in suspension cultures, and supports Baculovirus Expression Vector Systems (BEVS) for robust protein expression, including recombinant proteins and viral-like particles, as well as viral vector production.

Key benefits include:

• Animal component-free (ACF), serum-free medium supports high cell density, viability, and yield of recombinant proteins in Sf9 and Sf21 suspension cultures

• Low hydrolysate concentration provides consistent performance lot-to-lot

• Recombinant protein expression in a wide variety of expression systems, including BEVS

• Single, ready-to-use medium for long-term cultures

• cGMP manufactured to facilitate a seamless transition from research to commercial production

• Formulated for scalability, reliability, and quality

Achieve optimal production of recombinant proteins and viral vectors in Sf9 and Sf21 cells with an animal component-free, low hydrolysate medium

Figure 1. IS Sf Insect supports high viable cell density of Sf9 and Sf21 cultures

Sf9 and Sf21 cells were cultured in IS Sf Insect, or commercially-available media from Supplier 1 (ACF and AD) and Supplier 2 (ACF) for eight days. Viable cell density (VCD) was measured for Sf9 (A) and Sf21 (B) cells. Standard deviation of four replicate cultures represented.

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Advanced Formulation Reduces Cell Culture VariabilityYeast hydrolysate is commonly used to boost performance of cells, but variability between lots is a major factor leading to inconsistent performance between cell cultures. IS Sf Insect is serum-free, ACF, and features a low hydrolysate concentration to deliver more consistent performance between lots.

Reduced Hydrolysate Formula Delivers Increased Lot-to-lot Consistency

Figure 2. IS Sf Insect shows consistent performance between lots

Four lots of IS Sf Insect medium were manufactured using different lots of yeast hydrolysate in each lot of medium (Lots 1-4). Sf9 cells were cultured in Lots 1-4; viable cell density (VCD) (A) and maximum VCD (B) were measured for each lot and showed consistent performance. Data shown above represents the average of four replicate cultures. To measure statistical significance a two-way ANOVA test was performed on the Sf9 VCD data (A) and the results indicated yeast hydrolysate lots did not affect the VCD significantly (p=0.5005). A one-way ANOVA test was performed on the Sf9 maximum VCD data (B) and the results indicated yeast hydrolysate lots did not affect the maximum VCD significantly (p=0.3937).

The FUJIFILM Irvine Scientific Advantage

Stringent raw material qualification for ensuring

lot-to-lot consistency

Customized packaging including bags

(available upon request)

Customized media optimization and

development to meet specific customer needs

and applications

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IS Sf Insect Supports Efficient Protein Yields in Comparison to Other Commercially-available Serum-free Insect Cell Media

Designed to Facilitate a Seamless Transition from Research to Commercial Production

Figure 3. IS Sf Insect supports high infection rates by BEVS and protein yields that are comparable to commercially-available media

Sf9 and Sf21 cells were infected with BEVS expressing Vascular Endothelial Growth Factor (VEGF) on Day 0 (Sf9:MOI=0.1, Sf21: MOI=0.5). Infection efficiencies were measured by the percentage of cells expressing GFP through flow cytometry on Day 3 post-infection (A). The protein yields were measured by VEFG ELISA on Day 3 post-infection (B); data shown in B were normalized against the average VEGF value expressed in IS Sf Insect. Data shown represent four replicate cultures.

• cGMP manufactured for quality, consistency, and scalability

• Robust raw material controls and supply chain management

• Traceability documentation provided, including Certificates of Analysis, Certificates of Origin, and Drug Master Files (DMF) filed with the U.S. FDA

• Extensive QA testing, including functionality, sterility, and endotoxin

• Custom sizes and packaging available on request

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Discover the FUJIFILM Irvine Scientific Advantage With more than four decades of experience, we have cultivated deep industry knowledge, custom media expertise, superior customer service, and reliable results—positioning us as the cell culture media partner of choice for the biopharmaceutical, vaccine, and cell therapy industries.

FUJIFILM Irvine Scientific is focused on providing the highest quality cell culture media. We are dedicated to bringing life to products, and ultimately, to the patients who benefit from the resulting therapeutic advances. World-renowned for our unwavering commitment to full-spectrum partnership, FUJIFILM Irvine Scientific has defined the industry standard in support, innovation, and best-in-class turnaround time.

IS and the FUJIFILM Irvine Scientific logo are registered trademarks of FUJIFILM Irvine Scientific, Inc. in various jurisdictions. © 2019 Irvine Scientific P/N 000400 Rev.0

IS Sf Insect 91174 1 L Animal component-free, serum-free, low hydrolysate medium

Custom sizes and packaging available on request

Anti-clumping Supplement 91150 50 mL

Media Catalog# Size Additional Information