Disclosure to Promote the Right To Information

Whereas the Parliament of India has set out to provide a practical regime of right to information for citizens to secure access to information under the control of public authorities, in order to promote transparency and accountability in the working of every public authority, and whereas the attached publication of the Bureau of Indian Standards is of particular interest to the public, particularly disadvantaged communities and those engaged in the pursuit of education and knowledge, the attached public safety standard is made available to promote the timely dissemination of this information in an accurate manner to the public.

इंटरनेट मानक

“!ान $ एक न' भारत का +नम-ण”Satyanarayan Gangaram Pitroda

“Invent a New India Using Knowledge”

“प0रा1 को छोड न' 5 तरफ”Jawaharlal Nehru

“Step Out From the Old to the New”

“जान1 का अ+धकार, जी1 का अ+धकार”Mazdoor Kisan Shakti Sangathan

“The Right to Information, The Right to Live”

“!ान एक ऐसा खजाना > जो कभी च0राया नहB जा सकता है”Bhartṛhari—Nītiśatakam

“Knowledge is such a treasure which cannot be stolen”

“Invent a New India Using Knowledge”

है”ह”ह

IS 5654-1 (1970): Feeds for Laboratory Animals, Part I:Rats and Mice [FAD 5: Livestock Feeds, Equipment andSystems]

IS : 5654 ( Part I ) - 1970

Indian Standard

SPECIFICATION FOR

FEEDS FOR LABORATORY ANIMALS PART I RATS AND MICE

Animal Feeds Sectional Committee, AFDC 15

Chairman

DR K. K. IYA

Members

Representing

Indian Council of Agricultural Research, New Delhi

SHRI V. P. ANANTANARAYANAN Directorate of Marketing & Inspection ( Ministry of Food, Agriculture, Community Development

SHRI I,. K. SHUKLA ( Altersafe) & Co-operation ), Faridabad

DR I. S. BHATIA Punjab Agricultural University, Ludhiana SHRI ATMA SIN~H KOCAHAR ( Alternate )

SHRI N. BHOWMIK Directorate Genera1 of Technical Development, New Delhi

S~IRI R. D. KAWATRA ( A!ternole ) BRIG CHANDAN SINGR Directorate of Military Farms, Army Headquarters

LT-COL DILBAGH SIN~H ( Alternate ) ( Ministry of Defence )

DR H. B. CHAWLA Solvent Extractors’ Association of India, Bombay SARI P. V. VORA ( Alternate )

SHRI A. W. COURT Shaw Wallace & Company Ltd, Calcutta DR R. GANESH ( Alternate )

DR N. N. DASTUR National Dairy Research Institute, Karnal DR P. G. NAIR ( Alternate )

SHRI H. S. GURUDAS Voltas Limited, Bombay SERI N. J. AGA ( ‘Alternate )

HEAD OF THE ANIMAL NUTRITION Indian Veterinary Research Institute, Izatnagar DIVISION

JOINT COMMISSIONER ( LIVESTOCK Animal Husbandry Commissioner ( Ministry of PRODUCTION ) Food, Agriculture, Community Development

& Co-operation ) ASSISTANT COMMISSIONER (CATTLE FEEDS)( Alternate )

SHRI R. C. KHERA Indian Council of Agricultural Research, New Delhi SHRI F. G. T. MENEZES Directorate of Sugar & Vanaspati (Ministry of

Food, Agriculture, Community Development & Co-operation )

DEPUTY DIRECTOR ( VANASPATI ) ( Alternote ) DR B. PANDA Indian Veterinary Research Institute, Izatnagar DR V. N. PARGAONKAR Andhra Pradesh Agricultural

Hyderabad University,

DR C. V. REDDY ( A(tematc ) DR B. M. PATEL Institute of Agriculture, Anand

( Continued on fqqe 2 )

INDIAN STANDARDS INSTITUTION MANAK BHAVAN, 9 BAHADUR SHAH ZAFAR MARG

NEW DELHI 1

IS : 5654 ( Part I ) - 1970

( Continuedfrom page 1 )

Members Representing

DR A. P. SACH~EV All India Association of Poultry Industry, Bombay Du N. SAT,~PA~'HY Tata Oil Mills Co Ltd, Bombay

DR K. C. DANnoN. ( Alternate ) DR H. C. SAXENA Hindustan Lever Limited, Bombay

SHRI W. A. D. McF~nze.4N ( Alternate ) DR K. C. SEN In personal capacity ( 403 Jodhpur Park, Calcutta 31 ) SHRI v. H. SHAH Kaira District Co-operative Milk Producers’ Union

Ltd, Anand DR ( KUMA~I ) AMRITA P.~TEL ( Alternate )

SI~RI K. SHIVARA 1: {KRISHANAN Dairy Development Commissioner, Government of Maharashtra

DR B. SAHAJ ( Alternate ) SHRI G. V. SIRUII The Compound Livestock Feeds Manufacturers’

Association of India, Bombay SHKI W. .2. D. MCF~DZEAN ( Alternate )

SHRI VINEIZX~ VI~MANI Jawala Flour Mills, New Delhi SHIU ANANU VIRXXANI ( Alternate )

DR HARI BHAGWAN, Director General, IS1 ( Ex-oficio Member ) Deputy Director ( Agri & Food )

Secretary

SHRI G. S. VILKHU

Deputy Director ( ilgri & Food ): IS1

Feeds for Laboratory Animals Subcommittee, AFDC 15:6

Convener

Dn C. M. SINGH

Members

DR C. W. BROACH SIIHI T. G. COUTINHO Srrn~ M. K. GOVERDHAN DII A. E. KULKARNI DIG J. SAHA Drc S. R. SARVOT~IAM

DR K. S. VARADAN ( Alternate) DR H. C. SAXENA DR H. G. SEN

Indian Veterinary Research Institute, Izatnagar

Central Drug Research Institute ( CSIR ), Lucitnow Bhaba Atomic Research Centre, Bombay Virus Research Centre, Poona Haffkine Institute, Bombay Central Drugs Laboratory, Calcutta Hindustan Antibiotics Ltd, Poona

Hindustan Lever Limited, Bombay CIBA of India Limited, Bombay

2

IS:5654(Part I);1970

SPECIFICATION FOR FEEDS FQR LABORATORY ANIMALS

PART I RATS AND MICE

0. FOREWORD

0.1 This Indian Standard ( Part I ) was adopted by the Indian Standards Institution on 16 June 1970, after the draft finalized by the Animal Feeds Sectional Committee had been approved by the Agricultural and Food Products Division Council.

0.2 Various kinds of laboratory animals, such as rats, guinea pigs, ham- sters, rabbits and monkeys are finding an increasing use in modern biologi- cal research and testing of various drugs and pesticides. The animals maintained in the stock-colonies thrive and reproduce efficiently when fed on proper diet which is also necessary to ensure that the animals are free from pathogens. A series of Indian Standards is, therefore, being formulated to ensure availability of compounded feeds of suitable quality for feeding different laboratory animals. It is expected that these standards would be of assistance to the rapidly developing laboratory animal feeds industry.

0.3 The fungus AspergillusJlavus generally associated with groundnuts and oilcakes produces aflatoxin, the presence of which in laboratory animal feeds might affect the health of these animals thereby affecting results of the experiments in which they have been used. It is, therefore, recommend- ed that the users while procuring feeds for their use should ensure that their alfatoxin content is within safe limits.

0.4 Indian Standard codes of practice dealing with other aspects, such as care, breeding and management of laboratory animals, are being prepared separately. However, Indian Standards for compounded feeds for various laboratory animals are complementary to these codes.

0.5 ‘I’he ‘Indian Standard specification for feeds for laboratory animals’ will bc issued in several parts. This part ( Part I ) covers the requirements for the feeds for rats and mice. Specification covering the requirements for feeds for other laboratory animals are under preparation.

0.6 l:or the purpose of deciding whether a particular requirement of this staudard is complied with, the final v-alue, observed or calculated,

3

ISr5654( Part I)- 1970

expressing the result of a test, shall be rounded off in accordance with IS : 2 - 1960*. The number of significant places retained in the rounded off value should be the same as that of the specified value in this standard.

1. SCOPE 1.1 This standard ( Part I ) prescribes the requirements and the qethods of sampling and test for feeds for laboratory rats and mice.

2. REQUIREMENTS

2.1 Description - Compounded feeds for laboratory rats and mice shall be in the form of crumbs, cubes or pellets. The feed shall be free from rancidity, musty infestation.

odour, toxic ingredients, adulterants, moulds and insect

2.2 Compounded feeds for laboratory rats and mice shall also conform to the requirements prescribed in Table 1.

TABLE 1 REQUIREMENTS FOR COMPOUNDED FEEDS FOR LABORATORY RATS AND MICE

SL CHARACTERISTIC

( Clauses 2.2 and 5.1 )

REQUIREMENT METHOD OF TEST, No.

.(I) i)

ii)

iii)

iv)

vi)

vii)

viii)

(2) Moisture, percent by weight,

Max

Crude protein ( N x 6.25 ), percent by weight, &fin

Crude fat ( or ether extract ), percent by weight, Min

Crude fibre, percent by weight, Max

Total ash, percent by wright, Max

Acid insoluble ash, percent by weight, Max

Calcium ( as Ca ), percent by weight, Min

Available phosphorus, percent by weight, Min

(3) 10

24 C -

5

6

9

1.0

0.6

0.3

REF TO APPENDIX IN _____-____-_ IS : 1374-1968* IS : 1942-1968t

(4) (5.) B -

- D

D -

- E

- F

- B

K -

‘Specification for poultry feeds ( second revision ).

tSprcification for bone-meal as livestock feed supplement ( jrsf tevision ).

IS : 5654 ( Part I ) - 1970

2.3 Compounded feeds for laboratory rats and mice shall contain the fol- lowing in quantity not less than the value shown against each:

L-lysine, percent by weight, Min Methionine and cystine, percent by weight, Min

Tryptophan, percent by weight, Min L-arginine, percent by weight, Min

Essential fatty acids, percent by weight, Min

Vitamin A, IU/kg, Min

Vitamin D, IU/kg, Min Vitamin E, mg/kg

Riboflavin, mg/kg, Min

Calcium-D-pantothenate, mg/kg

Nicotinic acid ( amide ), mg/kg, Min

Vitamin B1 2, pg/kg, Min

Choline, mg/kg, Min

1-O

@8

0.2

0.2

0.2

2 700

200

40

6.0

20

60

10

1 100

2.3.1 The manufacturers shall declare on the label (see 3.2) the above- mentioned constituents.

2.4 Ingredients - The ingredients which may be used in the manufacture of fee&,for laboratory rats and mice are given in Appendix A.

2.4.1 Artificial ingredients, such as antibiotics, hormones or other drugs shall not be used in compounding feeds for laboratory animals.

3. PACKING AND MARKING

3.1 Packing - The feeds shall be packed in clean, sound, plain or polythene-lined jute or laminated paper bags. The mouth of each bag shall be either machine-stitched or rolled over and hand-stitched.

3.2 Marking - Each bag shall be suitably marked or labelled so as to give the following information:

a) Name of the material,

b) Name of the manufacturer,

c) Batch or code number,

d) Net weight,

e) Declaration ( see 2.3.1 ),

5

IS : 5654 ( Part I ) - 1970

f) List of ingredients, and

g) Date of manufacture.

3.2.1 Each bag may also be marked with the IS1 Certification Mark.

NOTE - The use of the IS1 Certification Mark is governed by the provisions of the Indian Standards Institution ( Certification Marks ) Act, and the Rules and Regula- tions made thereunder. Presence of this mark on products covered by an Indian Standard conveys the assurance that they have been produced to comply with the requirements of that standard, under a well-defined system of inspection, testing and quality control during production. IS1 and operated by the producer,

This system, which is devised and supervised by has the further safeguard that the products as

actually marketed are continuously checked by IS1 for conformity to the standard. Details of conditions, under which a licence for the use of the IS1 Certification Mark may be granted to manufacturers or processors, may be obtained from the Indian Standards Institution.

4. SAMPLING

4.1 Representative samples of the material shall be drawn according to the method prescribed in Appendix G of IS : 1374-1968*.

5. TESTS

5.1 Tests shall be carried out as prescribed in appropriate appendices specified in co1 4 and 5 of Table 1.

5.2 Quality of Reagents - Unless specified otherwise, pure chemicals shall be employed in tests and distilled water ( see IS : 1070-1960~ ) shall be used where the use of water as a reagent is intended.

NOTE - ‘Pure chemicals’ shall mean chemicals that do not contain impurities which affect the results of analysis.

APPENDIX A

( Clause 2.4 )

INGREDIENTS FOR FEEDS FOR LABORATORY RATS AND MICE

A-l. In the compounding of feeds for laboratory rats and mice a variety of ingredients is used. A list of some of such ingredients is as follows.

‘Sprcification for poultly feed ( spcorrrl revision ). tSpccification for water, distillrd quality ( reoisrd ).

6

IS : 5654 ( Part I ) -1970

Foodgrain and their By-Products

Foodgrains

Grain sievings

Maize bran, maize gluten and maize gluten feed

Rice bran or solvent extracted rice bran and polishings

Wheat bran

Oilcakes and Meals

Copra cake, coconut cake ( expeller-pressed or solvent extracted )

Cottonseed oil cake ( decorticated ) ( expeller-pressed or solvent extracted ) - up to 5 percent by weight

Groundnut oil cake ( expeller-pressed or solvent extracted )

GUAR ( Cyamopsis tctragonoloba ) meal -up to 5 percent by weight

Maize germ oil cake

Mustard oilcake

Safflower ( Carthumus tinctorius ) cake ( expeller-pressed or solvent extracted)

Sesamum ( Sesamum in&cum orientale ) cake ( expeller-pressed or solvent extracted )

Animal Products

Blood meal Fish meal Liver residue Meat meal and meat scrap Dried skimmed milk Casein

IS : 5654 ( Part I ) - 1970

Minerals, Vitamins and Supplements

Bonemeal ( steamed )

Calcium pentothenate

Choline chloride

Common salt

Dicalcium phosphate (fluorine content not to exceed O*O5 percent) Folic acid

Limestone

Niacin

Riboflavin supplement

Vitamin A supplement

Vitamin B1 2 supplement

Vitamin E supplement

Greens

Berseem ( Trifolium alexandrium ) leaf meal

Lucerne ( hledicago sativa ) leaf meal

8