Ion Chromatography - Universidad De Antioquiaquimica.udea.edu.co/~carlopez/cromatoion/Ion... · Ez-Lute ™ Buffer ... Ion Chromatography: Alltech vs. EPA Method 300.0 A0037 Analysis

Bulletin #380

NEW Application Guide: pp6-27

Columns:pp28 - 34

Starter Kits: p35

Standards & Buffers:pp36-37

Accessories:pp38-41

Sample Preparation:pp42-45

Instruments:pp45-65

Application and ProductSelection Guide

Ion Chromatography

carlopez carlopez

carlopez carlopez

carlopez carlopez

“Specialists in Chromatography”2

Contact your Alltech office or distributor for current or local prices.© Copyright 1998 Alltech Associates, Inc.

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Featured Products

Ion Chromatography Sample Preparation Products

Alltech is the industry leader in the sample preparation productsarea. Many sample preparation devices are available for solvingvarious interference problems in ion chromatography. The latestinnovation in this field is the SCAN™ (Sample Concentrator andNeutralizer)1000 Sample Processor. The SCAN 1000 is an on-line sample pretreatment device for neutralizing acidic and basicsamples before IC analysis. The same instrument can be usedas a preconcentration device for analyzing ppb and ppt levels ofionic species. Alltech continues to offer off-line SPE productssuch as Novo-Clean IC disks, Maxi-Clean IC cartridges, andExtract-Clean tubes.

Ion Chromatography Columns

Alltech offers the widest range of IC columns for both suppressor-based and single-column ion chromatography methods. TheAllsep Anion Column separates inorganic anions by suppressor-based or single-column IC methods. The new high capacityAllsep A-2 Anion Column separates organic and inorganic anionsin one run. Both columns can be used for the determination ofinorganic anions in water per US EPA Method 300.0. The AlltechUniversal Cation Column is excellent for separating mono &divalent cations and transition metals. A large assortment ofcolumns are available for analyzing anions, cations, organicacids, transition metals, anionic and cationic surfactants.

Total Customer Satisfaction

Alltech is known for our excellent technical staff and support in the chromatography industry. We offer many services to satisfyevery customer. Product recommendation, personalized method development, on-site installation and training, and continuousproduct support are some of the services we offer. Your complete satisfaction is guaranteed.

Ion Chromatography Accessories

A variety of IC accessories including tubing, fittings, tools,certified IC standards, EZ-lute buffer concentrates, and starterkits are available from Alltech for beginners or for experienced ICusers. The IC accessory starter kit contains all the essentialsupplies for smooth installation and operation of IC systems.Several new buffer concentrates have been recently introduced.The standards and buffer concentrates simplify your reagentpreparations. Different types of PEEK fittings including the newsuper Flange-Free Plus™ fittings are available for makingconnections. The Flange-Free Plus fitting system improves thepressure rating for connections to a standard 1/4-28 Port. Asimple, stainless-steel bushing helps compress the ferrule againstthe tubing for a secure seal to 5,000psig.

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Ion Chromatography Instrumentation

Alltech offers many unique instruments for ion chromatography.The Odyssey Ion Chromatography system is the most powerfuland versatile system available for running both suppressor-based and single-column IC methods. The 550 ConductivityDetector provides extraordinary detection sensitivity for all ionicspecies. The ERIS™1000HP Autosuppressor is the most reliableand versatile suppressor ever developed. The stable solid phasechemistry works with many mobile phases, methods, andinstruments. Add other HPLC detectors to convert the Odysseyinto an HPLC system. Add the new 527 Gradient Controller toform a high performance gradient system. For completeautomation and high volume applications, add the new 580Autosampler. All Alltech IC systems and components meet therequirements of US EPA Method 300.0.

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“Specialists in Chromatography” 3

Contact your Alltech office or distributor for current or local prices. © Copyright 1998 Alltech Associates, Inc.

Table of ContentsT

able of Contents

APPLICATIONS

Inorganic Anions .................................................... 6-13

Organic Anions .......................................................... 13

Organic Acids ....................................................... 14-15

Cations ................................................................. 16-20

Lanthanides ............................................................... 17

Transition Metals .................................................. 18-19

Metal Cyano-Complexes ........................................... 18

Cationic Amines ........................................................ 20

Anionic Surfactants ................................................... 21

Cationic Surfactants .................................................. 21

Trace Anions ........................................................ 22-23

Trace Cations ....................................................... 22-23

Complex Matrices................................................. 24-27

COLUMNS

Allsep......................................................................... 28

Allsep A-2 .................................................................. 28

Sarasep AN1 ............................................................. 29

Anion HC ................................................................... 29

Wescan Anion/R ....................................................... 30

Wescan Anion/S ........................................................ 30

Wescan Anion Exclusion ........................................... 31

Universal Cation ........................................................ 31

High Speed Universal Cation .................................... 31

Wescan Cation/R ...................................................... 32

Scavenger ................................................................. 32

Transition Metals ....................................................... 32

Surfactant .................................................................. 33

Surfactant/R .............................................................. 33

Guard Columns ......................................................... 34

STARTER KITS

Accessory Starter Kits ............................................... 35

Anion Starter Kits ...................................................... 35

Cation Starter Kits ..................................................... 35

STANDARDS

Anions ....................................................................... 36

Cations ...................................................................... 36

Organic Acids ............................................................ 37

Multi-Component Mixtures ........................................ 37

BUFFERS

Ez-Lute™ Buffer Concentrates ................................... 37

ACCESSORIES

Metal-Free Tubing ..................................................... 38

Tubing Cutter ............................................................ 38

High Pressure Metal-Free Fittings ............................ 39

Low Pressure Metal-Free Fittings ............................. 40

Flange-Free Plus Metal-Free Fittings ........................ 40

Injection Valves ......................................................... 41

Sample Loops ........................................................... 41

Plastic Syringes ......................................................... 41

Solvent Inlet and In-Line Filters ................................. 41

SAMPLE PREPARATION

Maxi-Clean IC....................................................... 42-43

Extract Clean IC ........................................................ 42

Novo-Clean IC ........................................................... 44

SCAN 1000 Sample Processor ............................ 45-47

INSTRUMENTS

SCAN 1000 Sample Processor ............................ 45-47

Model 550 Conductivity Detector ......................... 48-49

ERIS 1000HP Autosuppressor and Cells ............. 50-53

Model 335 Suppressor Module ................................. 54

Model 530 Column Heater ........................................ 55

Model 526 HPLC Pump ............................................ 56

Model 426 Pump ....................................................... 57

Model 301 HPLC Pump ............................................ 57

Model 527 Gradient Controller .................................. 58

On-Line Degassing System ...................................... 58

Linear 200 UV Detector ............................................. 59

Model 580 Autosampler ............................................ 60

Model 570 Autosampler ............................................ 61

TurboChrom Data Station ......................................... 62

Chromjet™ and Datajet™ Integrator ............................ 63

High Performance Odyssey IC System ..................... 64

Basic Odyssey IC System ......................................... 65

Fully Automated IC System ....................................... 65

INDICES

Analytes Index ........................................................... 66

Part Number Index .................................................... 67



Col

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Column Selection Guide, Technical Publications

S A M P L E

INORGANIC ANIONS CATIONS OTRANSITION METALS

Weak Acids Strong Acids Group I & II LanthanidesDivalent &Trivalent

MetalComplexes

Allsep A-2Column

AllsepColumn

Wescan Cation/RColumn

Universal CationColumn

AllsepColumn

Universal CationColumn

Wescan Anion /RColumn

Allsep A-2Column


Transition Metal C18Column

Transition Metal C18Column

AllsepColumn

Anion HCColumn


Wescan AnionExclusion Column

Wescan Anion/RColumn

Wescan Anion/SColumn

Recent PublicationsInstrumentation:

1. Raaidah Saari-Nordhaus, Lakshmy M. Nair, Robert M. Montgomery,and James M. Anderson, Jr., “ Electro-elution Ion and LiquidChromatography: Controlling Sample Retention by ElectrochemicallyGenerating or Modifying the Mobile phase” . J. Chromatogr. A, 782(1997) 69.

2. Raaidah Saari-Nordhaus and James M. Anderson, Jr.,“Electrochemically Regenerated Ion Suppressors, New Suppressorsfor Ion Chromatography” . J. Chromatogr. A, 782 (1997) 75.

3. Raaidah Saari-Nordhaus and Lakshmy M. Nair, “ElectrochemicallyRegenerated Ion Suppression (ERIS™) Improves and SimplifiesAnion and Cation Analyses by Suppressor-based IonChromatography” Lab Asia, 4 (3), (1997) 34.

4. Lakshmy M. Nair and Raaidah Saari-Nordhaus, “Use of a ConventionalHPLC System for Performing U.S. EPA Method 300.0, Determinationof Inorganic Anions by Ion Chromatography” Am. Lab. 29 (4) (1997)33FF.

5. Raaidah Saari-Nordhaus and James M. Anderson.,Jr.,“Electrochrmically Regenerated Solid-phase Suppressors for IonChromatography” Am. Lab. 28 (3) (1996) 33N.

6. Raaidah Saari-Nordhaus and James M. Anderson, Jr.,“IonChromatographic Analysis of Anions Usng Solid Phase ChemicalSuppressor” , Am. Lab. January. (1994) 28C.

Sample Preparation:1. Robert M. Mongomery, Raaidah Saari-Nordhaus and James M.

Anderson Jr., “ On-Line Sample Preparation Technique for IonChromatography”. J. Chromatogr. A, In Press (1998).

2. Raaidah Saari-Nordhaus and James M. Anderson, Jr., “Membrane-based Solid-Phase Extraction as a Sample Clean-Up Technique forAnion Analysis by Capillary Electrophoresis” J. Chromatogr. A, 706(1995) 563.

3. Raaidah Saari-Nordhaus, Lakshmy M. Nair, and James M. Anderson,Jr., “Elimination of Matrix Interferences in Ion Chromatography bythe Use of Solid-Phase Extraction Disks” J. Chromatogr. A, 671(1994) 159.

3. Inga K. Henderson, Raaidah Saari-Nordhaus, and James M.Anderson, Jr., “ Sample Preparation for Ion Chromatography”, J.Chromatogr., 546 (1991) 61.

4. Raaidah Saari-Nordhaus, James M. Anderson, Jr., and Inga K.Henderson, “Sample Preparation for Ion Chromatography usingSolid Phase Extraction”, Int. Lab., October (1990), 36.

5. Raaidah Saari-Nordhaus, James M. Anderson, Jr., and Inga K.Henderson, “Sample Preparation for Ion Chromatography usingSolid Phase Extraction”, Am. Lab., 22 (12) (1990), 18.



Column Selection Guide, Technical Publications, Application NotesC

olumn S

election Guide

Ion Chromatography Application Notes

A0001 A Complete Listing of All Application NotesA0002 Analysis of Anions Using p-Hydroxybenzoic AcidA0003 Analysis of Anions Using Phthalic Acid EluantA0004 Analysis of Weak Acid AnionsA0005 Analysis of Organic Acids by Anion Exclusion

ChromatographyA0006 Analysis of Monovalent Cations Using Conventional

Cation Exchange ColumnA0007 Analysis of Divalent Cations Using Conventional

Cation Exchange ColumnA0008 Determination of Aluminum by Ion ChromatographyA0009 Analysis of Divalent Transition Metals with

Conductivity DetectionA0010 Analysis of Divalent Transition Metals with Post

Column ReactionA0011 Analysis of Hydroxyquat, Monoquat,and DiquatA0012 Simultaneous Analysis of Monovalent and Divalent

CationsA0013 Analysis of Anions Using Carbonate/Bicarbonate

Mobile PhaseA0014 Ion Chromatographic Analysis of LanthanidesA0015 Analysis of Metal Cyano Complexes by Ion

Interaction ChromatographyA0016 Analysis of OxalateA0017 Anions in ToothpasteA0018 Anions in AnilineA0019 Analysis of Cationic SurfactantsA0020 Analysis of Phosphate Species Using ICA0021 Analysis of Anionic SurfactantsA0022 Determination of Iodide and Other Late Eluting

Anions by ICA0023 Determination of Dibutylphosphoric Acid by SCICA0024 Analysis of AminesA0025 Temperature Effects on Conductivity Detection in

Single Column Ion ChromatographyA0026 Analysis of Azide by Ion ChromatographyA0027 The Analysis of Fatty Acids by Ion ChromatographyA0028 Transition Metal Analysis Using PBDMA Stationary

PhaseA0029 Determination of Haloacetic Acids by ICA0030 The Analysis of Inorganic and Organic Ions in Food

Products by Ion ChromatographyA0031 Analysis of Arsenate, Selenite, Selenate,

Tungstate, & Molybdate by Suppressor-based ICA0032 Analysis of Bicarbonate/Carbonate by ICA0033 Analysis of Alkanesulfonate and Alkyl Suflate

Surfactants by Ion ChromatographyA0034 Hydrazine AnalysisA0035 On-Line Sample Preparation with Electrochemically

Regenerated Ion Suppressor (ERIS™)A0036 Determination of Inorganic Anions in Reagent Water by

Ion Chromatography: Alltech vs. EPA Method 300.0A0037 Analysis of Sulfite and Sulfate by Suppressor-Based ICA0038 Analysis of Guanidine and L-Arginine by IC

A0039 Analysis of Plating Bath Solutions by IC

Call and request Application Notes orview on our website.

ORGANIC ACIDS SURFACTANTS

Anionic Cationic

Surfactant/RColumn

Surfactant/RColumn

Wescan AnionExclusion Column

Surfactant C8 & C18Column

AllsepColumn

Allsep A-2Column

Columns and Methods:1. Lakshmy M. Nair and Raaidah Saari-Nordhaus., “Recent Developments

in Surfactant Analysis by Ion Chromatography” J. Chromatogr. A, InPress (1998).

2. Lakshmy M. Nair, Raaidah Saari-Nordhaus and Robert M. Montgomery.,“Applications of a New Methacrylate-Based Anion Stationary Phase forthe Separation of Inorganic Anions” J. Chromatogr. A, 789 (1997) 127.

3. Lakshmy M. Nair, Brian R. Kildew, and Raaidah Saari-Nordhaus.,“Enhancing the Anion Separations on a Polydivinylbenzene-basedanion stationary phase” J. Chromatogr. A, 739 (1996) 99.

4. Lakshmy M. Nair, Raaidah Saari-Nordhaus, and James M. Anderson,Jr., “Ion Chromatographic Separation of Transition Metals on aPolybutadiene Maleic Acid-Coated Stationary Phase” J. Chromatogr. A,671 (1994) 43.

5. Lakshmy Nair, Raaidah Saari-Nordhaus, and James M. Anderson, Jr.,“Simultaneous Analysis of Alkali and Alkaline Earth Cations onPolybutadiene-Maleic Acid Coated Stationary Phase Using Mineral AcidEluants”, J. Chromatogr., 640 (1993) 40.

6. Raaidah Saari-Nordhaus and James M. Anderson, Jr.,“IonChromatographic Separation of Inorganic Anions and Carboxylic Acidson a Mixed-Mode Stationary Phase”, Anal. Chem.,64 (1992) 2283.


App

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s Anion Analysis

Ion chromatography (IC) is an indispensable and superior methodfor anion analysis. Alltech offers instruments, columns andmethods for analyzing inorganic anions. Other than thechromatograms and conditions shown in this catalog, there areseveral application notes available for specific methods. AlltechIC application’s specialists are only one phone call away for anyindividual applications to customize your needs.

Fast and Sensitive Analysis ofCommon Inorganic Anions 8542

Column: Allsep Anion, 100 x 4.6mm (Part No. 51200)Mobile Phase: 0.85mM NaHCO3/0.9mM Na2CO3

Flowrate: 2.0mL/minDetector: Suppressed Conductivity

1. Fluoride (20ppb)2. Chloride (40ppb)3. Nitrite-N (12ppb)4. Bromide (40ppb)5. Nitrate-N (9ppb)6. Phosphate-P (19ppb)7. Sulfate (60ppb)

The Allsep™ column separates common inorganic anions in thesingle-column IC mode using a p-hydroxybenzoic acid mobilephase. This method detects ppm level anions and is also the onlysuitable method for measuring carbonate or bicarbonate in varioussamples.

The Allsep™ Anion column separates low ppb level anions in lessthan 10 minutes. Use the Allsep Column for EPA Method 300.0 (Part'A') analysis of ground water, raw water, drinking water, waste waterand solid waste extracts. Use this same method for other matricescontaining common inorganic anions.

At pH 3.8 to 4.5 phthalic acid mobile phase is commonly used foranion analysis by single-column IC. In this pH range, samplecarbonate and hydroxide are neutralized and won’t interfere withthe analysis of other anions. This mobile phase is also useful foranalyzing late eluting anions such as oxalate and thiosulfate.

Common Inorganic Anionsand Carbonate

Column: Allsep Anion, 100 x 4.6mm (Part No. 51200)Mobile Phase: 4mM p-Hydroxybenzoic Acid, pH 7.5 with LiOHFlowrate: 1.0mL/minDetector: Conductivity

1. Fluoride (1ppm)2. Carbonate (5ppm)3. Chloride (2ppm)4. Nitrite (2ppm)5. Bromide (2ppm)6. Nitrate (2ppm)7. Phosphate (3ppm)8. Sulfate (3ppm)

Common and Late Eluting Anions8461

Column: Allsep Anion, 100 x 4.6mm (Part No. 51200)Mobile Phase: 4mM Phthalic Acid, pH 4.2 with LiOHFlowrate: 1.0mL/minDetector: Conductivity

1. Fluoride (9ppm)2. Chloride (18ppm)3. Nitrite (18ppm)4. Bromide (18ppm)5. Nitrate (18ppm)6. Oxalate (50ppm)7. Sulfate (27ppm)8. Thiosulfate (50ppm)

Sodium octane sulfonate is a strong mobile phase which can elutepolarizable anions such as iodide, thiocyanate and thiosulfatealong with other anions. This method can be adapted to all industriesthat require the determination of iodide, thiocyanate and thiosulfate.

Strongly Retained Anions

Column: Allsep Anion, 100 x 4.6mm (Part No. 51200)Mobile Phase: 1mM Sodium Octane SulfonateFlowrate: 1.5mL/minDetector: ConductivityColumn Temp: 45°C

1. Fluoride (2ppm)2. Chloride (2ppm)3. Nitrate (5ppm)4. Iodide (20ppm)5. Sulfate (5ppm)6. Thiocyanate (20ppm)7. Thiosulfate (20ppm)

Succinic acid is a weak aliphatic carboxylic acid and at pH 3.0, it willelute only monovalent anions. At this pH, the phosphate exists asa monovalent anion and elutes before chloride and nitrite. Thiscondition is ideal for phosphate speciation.

Phosphate Speciation

Column: Allsep Anion, 100 x 4.6mm (Part No. 51200)Mobile Phase: 20mM Succinic Acid, pH 3.0 with LiOHFlowrate: 1.5mL/minDetector: Conductivity

1. Phosphate (10ppm)2. Phosphite (20ppm)3. Hypophosphite (20ppm)4. Chloride (4ppm)5. Nitrite (8ppm)

8581

8596

8496

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Inorganic Anions


Pyrophosphate is a very strongly retained phosphate species. Itcan be eluted with trimesic acid, which is a strong aromatic carboxylicacid mobile phase.

Pyrophosphate and Other Anions

The Allsep Anion Column and the Alltech Odyssey™ IonChromatography System are powerful tools for plating-bath analysis.Simultaneous separation of hexafluorosilicate (SiF62-), nitrite, sulfate,and chromate in plating bath is possible by this method.

Chromate Plating Bath

Sodium-azide nitrogen generators are used to inflate automobile airbags. Trace amounts of unreacted azide left in the inflator aredangerous to both passengers and car industry workers. IC is anexcellent method for monitoring azide levels in such matrices.Perchlorate can also be analyzed using this method.

Azide, Nitrate and Perchlorate

Column: Allsep Anion, 100 x 4.6mm (Part No. 51200)Mobile Phase: 5mM Trimesic AcidFlowrate: 1.0mL/minDetector: Conductivity

1. Fluoride (2ppm)2. Phosphate (6ppm)3. Chloride (4ppm)4. Nitrite (4ppm)5. Bromide (4ppm)6. Nitrate (4ppm)7. Pyrophosphate (20ppm)8. Sulfate (6ppm)

Column: Allsep Anion, 100 x 4.6mm (Part No. 51200)Mobile Phase: 0.85mM NaHCO3/ 0.9mM Na2CO3


1. Hexafluorosilicate2. Nitrite3. Sulfate4. Chromate

Column: Allsep Anion, 100 x 4.6mm (Part No. 51200)Mobile Phase: 4mM Phthalic Acid, pH 4.2 with LiOHFlowrate: 1.6mL/minDetector: Conductivity

1. Azide2. Nitrate3. Perchlorate

Monofluorophosphate (MFP) and fluoride are anti-caries agents inoral care formulations. Over time, MFP can breakdown to phosphateand fluoride. IC is the best method for the simultaneous separationof MFP and fluoride in toothpaste and other oral hygenic products.

Toothpaste

Iodate is a preservative in bakery products while bromate is ableaching and conditioning agent in bakery dough. The AllsepAnion is the column of choice for the separation of iodate andbromate in bakery products.

Bakery Additives

A mixture of tributylphosphoric acid (TBP) and kerosene is used toextract uranium from the reactor fuel and fission products. Dibutyl(DBP) and monobutyl phosphoric acids are degradation byproductsof this process. IC is an ideal method for monitoring DBP content inthis process.

Nuclear Power Plant Extract





1. Fluoride2. Chloride3. Phosphate4. Monofluorophosphate

Column: Allsep Anion, 100 x 4.6mm (Part No. 51200)Mobile Phase: 5mM Trimesic AcidFlowrate: 1.2mL/minDetector: Conductivity

1. Iodate2. Bromate

1. Phosphate2. Dibutylphosphate3. Monobutylphosphate

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Inorganic AnionsA

pplications


Simultaneous separation of weakly retained iodate and stronglyretained iodide species is usually difficult. The Allsep AnionColumn with suppressed conductivity detection provides an easymethod for the determination of iodide and iodate.

Iodide and Iodate8601

Single-Column IC is the method of choice for analyzing arsenite.Suppressed conductivity detection is not suitable for this applicationsince the suppressor converts arsenite to a non-conductivearsenious acid.

Arsenite and Arsenate8600

Determination of weakly ionized carboxylic acids is often problematicbecause of co-elution with each other or with other inorganicanions. These ions are often misinterpreted as fluoride. The highcapacity Allsep A-2 Anion Column separates these ions fromfluoride and chloride.

Weak Acid Anions8743

Selenate and arsenate can be found in soil samples. Tungstateenters the water and soil via dust produced in the production ofalloy steels. Molybdate is essential for enzyme functioning inplants. The determinations of these anions are important due totheir biological effects on the environment.

Selenite, Selenate, Arsenate,Tungstate and Molybdate 8610

Aspartame is a sugar substitute that can be analyzed by ionchromatography. This method is an alternative to HPLC with UVdetection method, which often suffers from interference of dyesand flavors.

Diet Cola8458

Quantification of oxalate in urine is important to diagnose kidneystone disease. Oxalate in urine can be successfully analyzed by ICin less than 20 minutes. Only dilution and filtration were used assample preparation.

Oxalate in Urine8451



Column: Allsep Anion, 100 x 4.6mm (Part No. 51200)Mobile Phase: 4mM Phthalic Acid, pH 4.2Flowrate: 1.2 mL/minDetector: Conductivity





Column: Allsep Anion, 100 x 4.6mm (Part No. 51200)Mobile Phase: 4mM Phthalic Acid, pH 4.2Flowrate: 1.2mL/minDetector: Conductivity

Column: Allsep A-2 Anion, 100 x 4.6mm(Part No. 51218)

Mobile Phase: 2.1mM NaHCO3/1.6mM Na2CO3

Flowrate: 2.0 mL/minDetector: Suppressed Conductivity

1. Fluoride2. Chloride

6. Phosphate7. Selenite8. Sulfate9. Arsenate

10. Selenate11. Tungstate12. Molybdate

1. Aspartame2. Chloride3. Nitrate4. Sulfate

1. Chloride2. Nitrate3. Phosphate4. Sulfate5. Oxalate

1. Iodate (20ppm)2. Iodide (20ppm)

1. Arsenite (40ppm)2. Arsenate (40ppm)

1. Fluoride (1ppm)2. Acetate (1ppm)3. Formate (1ppm)4. Chloride (1ppm)

0

21

34 5 6

78

910 11 12

10 20 30 40 Min.

3. Nitrite4. Bromide5. Nitrate

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App

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sInorganic Anions

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Simultaneous separation of selenite and selenate, arsenite andarsenate is difficult under most IC conditions. The Wescan AnionExclusion Column can separate these ions under acidic conditions.Detection is by UV detector at 210nm.

Phosphate and phosphite are difficult to separate, but often foundin the same sample. A longer Allsep Anion Column with a weaksodium bicarbonate mobile phase can separate these species.

Phosphate and Phosphite8708

Tetrafluoroborate is formed when borate is reacted with hydrofluoricacid. Sodium octane sulfonate and the Allsep column provide aneasy method for tetrafluoroborate analysis.

Tetrafluoroborate8608

Arsenic and Selenium Speciation8705

EDTA is a strongly retained anion with a -4 charge. A highconcentration bicarbonate/carbonate mobile phase with the additionof p-cyanophenol can elute EDTA from the Allsep column.

EDTA8609

This method shows the separation of Iron (III) without the interferenceof other ions. The EDTA mobile phase complex with iron to formEDTA-iron which is UV absorbing at 330nm.

Iron (III)8704

Bromate is used as a bleaching and conditioning agent in bakeryproducts. Sodium perchlorate mobile phase with indirect UVdetection provides an excellent method for analyzing bromate andbromide when other ions are not analytes of interest.

Bromate and Bromide8698

Column: Allsep Anion, 100 x 4.6mm (Part No. 51200)Mobile Phase: 2.8mM NaHCO3/

2.2mM Na2CO3 in 1mM p-CyanophenolFlowrate: 1.2mL/minDetector: Suppressed Conductivity

Column: Allsep Anion, 100 x 4.6mm (Part No. 51200)Mobile Phase: 2mM EDTAFlowrate: 1.2mL/minDetector: UV at 330nm

Column: Allsep Anion, 150 x 4.6mm (Part No. 51208)Mobile Phase: 1mM Sodium PerchlorateFlowrate: 1.0mL/minDetector: Indirect UV at 215nm

Column: Allsep Anion, 150 x 4.6mm (Part No. 51208)Mobile Phase: 8mM Sodium BicarbonateFlowrate: 1.6mL/minDetector: Suppressed Conductivity

Column: Allsep Anion, 100 x 4.6mm (Part No. 51200)Mobile Phase: 1mM Sodium Octane SulfonateFlowrate: 1.5mL/minDetector: Conductivity

Column: Wescan Anion Exclusion, 300 x 7.5mm(Part No. 269062)

Mobile Phase: 10mM Phosphoric acidFlowrate: 1.0mL/minDetector: UV at 210nm

1. EDTA (20ppm)

1. Iron III (10ppm)

1. Bromate (10ppm)2. Bromide (10ppm)

1. Tetrafluoroborate (5ppm)2. Sulfate (5ppm)

1. Phosphate (30ppm)2. Phosphite (30ppm)

0 4

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1. Selenate (20ppm)2. Arsenate (50ppm)3. Selenite (20ppm)4. Arsenite (50ppm)

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24 Min.0 4 8 12 16 20

The Sarasep AN1 column provides a fast method for analyzingsulfite and other anions. This column resolves sulfite away fromsulfate.

Sulfite with Other Anions

Selenium is an essential nutrient or can be a serious hazardouselement. The difference between essential and toxic concentrationsis rather narrow. Selenium species can be analyzed easily by IC.

Selenite, Selenate andOther Anions

Hypophosphite is added to nickel plating solution as a reducingagent for the reduction of metal ions to metal. Both nickel andhypophosphite are exhausted in the plating process. Hypophosphiteis converted to phosphite and to control the plating bath,phosphorous species needs to be analyzed.

Nickel Plating Solution

The high-capacity Anion HC Column separates common anionsand oxyhalides in one run. Use this method for routine environmentalmonitoring of drinking water, ground water, waste water, andbrines.

Inorganic Anions and Oxyhalides(EPA 300B)

Presence of high chloride concentration in sea water sample causesthe bromide and nitrate separation to be extremely difficult. Thehigh capacity Anion HC Column can separate bromide and nitrateaway from chloride, simplifying bromide and nitrate quantificationin sea water.

Anions in Sea Water

Ozone treated water contains byproducts such as bromate, chlorate,and nitrate. Because of its unique selectivity, the Anion HC Columnseparates low level bromate in the presence of high chlorideconcentrations.

Ozone-treated Water

6316

8611

8454

7870

7883

7944

Column: Anion HC Column, 150 x 4.6mm (Part No. 27410)Mobile Phase: 2.8mM NaHCO3/2.2mM Na2CO3 in 10% MethanolFlowrate: 1.2mL/minDetector: Suppressed Conductivity

Column: Anion HC, 150 x 4.6mm (Part No. 27410 )Mobile Phase: 2.8mM NaHCO3/2.2mM Na2CO3 in 10% MethanolFlowrate: 1.2mL/minDetector: Suppressed Conductivity

Column: Anion HC, 150 x 4.6mm (Part No. 27410)Mobile Phase: 1.4mM NaHCO3/1.1mM Na2CO3 in 5% MethanolFlowrate: 1.4mL/minDetector: Suppressed Conductivity

Column: Sarasep AN1 Column, 250 x 4.6mm(Part No. 38140)



Column: Sarasep AN1 Column, 250 x 4.6mm(Part No. 38140)



Column: Allsep Anion, 100 x 4.6mm, (Part No. 51200)Mobile Phase: 0.85mM NaHCO3/0.9mM Na2CO3


1. Fluoride (2.0ppm)2. Chlorite (2.0ppm)3. Bromate (2.0ppm)

4. Chloride (2.0ppm)5. Nitrite (2.0ppm)6. Phosphate (2.0ppm)7. Bromide (2.0ppm)8. Chlorate (2.0ppm)9. Nitrate (2.0ppm)

10. Sulfate (2.0ppm)

1. Chloride2. Bromide3. Nitrate4. Sulfate

1. Bromate2. Chloride3. Chlorate4. Nitrate

1. Hypophosphite2. Phosphate & Phosphite3. Sulfate

1. Fluoride2. Chloride3. Nitrite

4. Bromide5. Nitrate6. Selenite7. Phosphate8. Sulfate9. Selenate

1. Water2. Fluoride3. Chloride

4. Nitrite5. Bromide6. Nitrate7. Phosphate8. Sulfite9. Sulfate

0 5 10 15 20 Min.

10987653

2

1 4

1

23

4

5 6

7

8 9

0 2 4 6 8 10 12 14 16 Min.

0 5 10 15 20 25 Min.

1

2

3

4

0 4

1

2

3

8 12 16 20 24 Min.0 5 10 15 20 25 Min.

431

2

0 4

123

4 5

67

8

9

8 12 16 Min.

App

lica

tion

sInorganic Anions


Nickel sulfamate bath contains high concentrations of sulfamic andboric acids. Traditionally two separation methods, ion exchangeand ion exclusion, were used for the separation of strongly ionizedsulfamate and weakly ionized borate. The Wescan Anion/R Columnseparates both ions in one run.

Nickel Sulfamate Bath

Sulfite and its oxidation products, sulfate and thiosulfate arecomponents of pulping liquors. Thiosulfate analysis is especiallyimportant to control the liquor composition so any corrosionproblems will be minimized.

Sulfite, Sulfate and Thiosulfate

Pharmaceutical samples such as IV Solution must be monitoredclosely for integrity reasons. The Wescan Anion/R column canseparate acetate, lactate, and chloride in IV solution in thesuppressor-based IC mode.

Intravenous (IV) Solution

Weak acids are present in antifreeze, waste water, industrial productsand clinical samples. Single-column IC is the only method foranalyzing weak acid anions. Weak acids are converted to non-conductive ions in the suppressor, eliminating suppressor-basedIC as a detection method.

Weak Acid Anions

Separation of sulfite in the presence of sulfate is difficult butimportant in many applications. Sulfite is a very unstable ion and itoxidizes easily to sulfate. The Anion/R Column can separate sulfiteand sulfate along with other common anions.

Sulfite and Other Anions

Copper electroless plating bath contains both EDTA ion and copperEDTA complex. The Wescan Anion/R column can separate both ofthese species in the Single-Column IC mode.

Electroless Copper Bath

5943

8559

8604

5697

6397

5938

Column: Anion/R, 250 x 4.1mm, (Part No. 269029)Mobile Phase: 4mM Sodium Hydroxide/0.1mM Sodium BenzoateFlowrate: 1.5mL/minDetector: Conductivity

Column: Anion/R, 100 x 4.1mm, (Part No. 269031)Mobile Phase: 1.7mM NaHCO3/1.8mM Na2CO3


Column: Wescan Anion/R, 100 x 4.1mm (Part No. 269031)Mobile Phase: 5mM Sulfuric AcidFlowrate: 1.0mL/minDetector: Conductivity

Column: Anion/R, 100 x 4.1mm, (Part No. 269031)Mobile Phase: 4mM Sodium Hydroxide/0.1mM Sodium BenzoateFlowrate: 1.5mL/minDetector: Conductivity

Column: Anion /R, 100 x 4.6mm, (Part No. 269036)Mobile Phase: 1.7mM NaHCO3/1.8mM Na2CO3 in

0.3mM p-CyanophenolFlowrate: 2.5mL/minDetector: Suppressed Conductivity

Column: Anion/R, 250 x 4.1mm,(Part No. 269029)Mobile Phase: 5mM Sodium HydroxideFlowrate: 1.5mL/minDetector: Conductivity

1. Borate (5ppm)2. Silicate (10ppm)3. Formate (10ppm)4. Sulfide (10ppm)5. Chloride (20ppm)6. Cyanide (20ppm)

1. Borate2. Sulfamate

1. Fluoride (2.5ppm)2. Carbonate3. Chloride (5ppm)4. Nitrite (5.0ppm)5. Bromide (5.0ppm)6. Phosphate (8.5ppm), Nitrate (5ppm)7. Sulfite (5.0ppm)8. Sulfate (8.5ppm)

1. EDTA2. CuEDTA

1. Acetate (10ppm)2. Lactate (10ppm)3. Chloride (10ppm)

1

23

456

0 9 18 Min.

1

2

0 2 4 Min.

0 5

1

2

3

10 15 20 Min.0 5 10 15 20 Min.

1 3

4

56

7

8

2

1

2

0 3 6 Min. 0 5

12

3

10 15 20 25 Min.

1. Sulfite2. Sulfate3. Thiosulfate

Inorganic AnionsA

pplications



Certain sulfocarboxylic acids are present in detergents and cleansingproducts. Single-column ion chromatography is an excellent methodfor analyzing various sulfocarboxylic acids.

Sulfocarboxylic Acids

Thiocyanate is a metabolite of cyanide. Therefore cyanidedeterminations can be made indirectly by determining thiocyanatelevels. Monitoring thiocyanate in urine helps to determine theexposure of cyanide at work place.

Thiocyanate in Urine

The High capacity Anion/R column separates polyphosphates andphosphonates using acidic mobile phase and post columnderivatization with UV/VIS detection.

Phosphate and Phosphonate Species

Monitoring of scrubber solutions in flue gas desulfurization liquorsis an important application. The scrubber solutions contain differentsulfur species that can be analyzed by IC.

Flue Gas Desulfurization Liquors

Chelating agents such as EDTA are difficult to analyze because theyare polyvalent and retain strongly onto the ion-exchange resins.However, EDTA can be analyzed using the Wescan Anion/S columnwith a sulfuric acid mobile phase. EDTA is present as a monovalentanion with the acidic mobile phase.

EDTA

Cyclamate is an artificial sweetener. It is extremely non chromophoricwhich makes it a good candidate to detect by conductivity. It can beanalyzed in a diet cola sample by ion chromatography.

Cyclamate in Soft Drink

5889

8605

8573

5911

8284

5888

Column: Wescan Anion/S, 100 x 4.6mm (Part No. 269013)Mobile Phase: 3mM Phthalic Acid, pH 3.8Flowrate: 2.7mL/minDetector: Conductivity

Column: Wescan Anion/S, 100 x 4.6mm (Part No. 269013)Mobile Phase: 1.5mM Sulfuric AcidFlowrate: 1.0mL/minDetector: UV at 210nm

Column: Wescan Anion/S, 250 x 4.6mm (Part No. 269001)Mobile Phase: 5mM Phthalic Acid, pH unadjustedFlowrate: 1.6mL/minDetector: Conductivity

Column: Wescan Anion/S, 250 x 4.6mm (Part No. 269001)Mobile Phase: 10mM Phthalic Acid, pH unadjustedFlowrate: 2.3mL/minDetector: Conductivity

Column: Wescan Anion/S, 100 x 4.6mm (Part No. 269013)Mobile Phase: 5mM Phthalic Acid, pH 5.0 with KOHFlowrate: 4.0mL/minDetector: Conductivity

1. Chloride2. Nitrate3. Sulfate4. Hydroxylamine Disulfonate5. Dithionate

1. Chloride2. Sulfoacetate3. Sulfopropionate4. Sulfosuccinate

1. System peak2. Thiocyanate

1. Nitrite (50ppm)2. EDTA (100ppm)3. Nitrate (50ppm)4. Bromide (50ppm)

1. Chloride2. Cyclamate

1

2

3

4

5

0 5 10 15 20 Min.

1

4

23

0 3 6 9 12 Min.

0

2

1

10 20 Min.0 4 8 12 16 20 24 Min.

1

2

3 4

0 5 10 15 20 Min.

1

2

Column: Wescan Anion/R, 250 x 4.1mm(Part No. 269029)

Mobile Phase: 10mM Nitric AcidPost Column Reagent: 2.5mM Ferric Nitrate/2% Perchloric acidReagent Flowrate: 0.5mL/minFlowrate: 1.0mL/minDetector: UV at 330nm

1. Phosphate2. Hydroxyethylidene-1,1

Diphosphonic Acid (HEDP)3. 2-phosphonobutane-1,2, 6

Tricarboxylic Acid (PBTC)4. Pyrophosphate0 3

1 23

4

6 9 12 Min.

App

lica

tion

sInorganic Anions


Ion-exchange chromatography is useful for the separation of organicand inorganic anions in one run. For example, samples containingoxalate and inorganic anions can be analyzed by a single method asshown in this chromatogram.

Oxalate and Other Anions

Organic acids are weakly ionized species that are retained on anAnion Exclusion Column. Strongly ionized inorganic anions areexcluded from the pores of the resin and will elute in the voidvolume. This technique is ideal for analyzing organic acids insamples containing highly ionized inorganic anions.

Organic Acid Standards

Trifluoroacetate is used to cleave peptides from solid-phase resinsduring peptide synthesis. The presence of both acetate andtrifluoroacetate can be determined by anion exchange or anionexclusion chromatography.

Peptide Sample

Separation of weak and strong acid anions in one run simplifiessample analysis. Generally this would have to be done by twodifferent methods or with gradient elution. However, the AllsepA-2 Anion Column has the capacity to separate weak organic anionsand strong acid anions in one run.

Isocratic Separation of Weakand Strong Acid Anions

Haloacetic acids are present in many samples due to their use invarious industrial applications. Trace amounts of these acids arepresent in drinking water as chlorination byproducts. Ion exchangeand ion exclusion can be used for these acids.

Chloroacetic Acids by Ion Exchange

Traditionally organic acids are analyzed by ion-exclusion.Trichloroacetic acid is highly ionized and will therefore elute morerapidly than the other chloroacetic acids.

Chloroacetic Acids by Ion Exclusion

8602

6061

8579

8742

8574

6392

Column: Allsep A-2 Anion, 100 x 4.6mm (Part No. 51218)Mobile Phase: 2.1mM NaHCO3/ 1.6mM Na2CO3




Column: Alltech Anion Exclusion, 300 x 7.8mm(Part No. 269006)

Mobile phase: 1% Phosphoric AcidFlowrate: 0.8mL/minDetector: UV at 210nm



Column: Anion Exclusion, 100 x 7.5mm (Part No. 269068)Mobile Phase: 1mM Sulfuric AcidFlowrate: 0.6mL/minDetection: Conductivity

Column: Allsep Anion, 100 x 4.6mm (Part No. 51200)Mobile Phase: 0.85mM NaHCO3/ 0.9mM Na2CO3

Flowrate: 1.2mL/minDetection: Suppressed Conductivity

1. Trichloroacetic Acid2. Dichloroacetic Acid3. Acetic Acid4. Monochloroacetic Acid

1. Acetate2. Trifluoroacetate

1. Oxalic Acid2. Maleic Acid3. Malic Acid4. Succinc Acid5. Formic Acid6. Acetic Acid

1. Acetate (5ppm)2. Monochloroacetate (5ppm)3. Dichloroacetate (5ppm)4. Trichloroacetate (50ppm)

1. Fluoride (1ppm)2. Acetate (1ppm)3. Formate (1ppm)4. Chlorite (1ppm)

5. Chloride (2ppm)6. Nitrite (2ppm)7. Bromide (2ppm)8. Chlorate (5ppm)9. Nitrate (2ppm)

10. Phosphate (3ppm)11. Sulfate (3ppm)

1. Fluoride (4ppm)2. Chloride (8ppm)3. Nitrite (8ppm)4. Bromide (8ppm)5. Nitrate (8ppm)6. Phosphate (12ppm)7. Sulfate (12ppm)8. Oxalate (20ppm)

0 4

1 2

34

56

7

8

8 12 16 20 Min.

12

45

6

3

0 10 20 Min.0 8

1

2

34

16 24 32 Min.

0 5 10 15 Min.

34

2

1

0 2

12

4 6 8 10 12 Min.

Organic and Inorganic AnionsA

pplications0 4

1

2

3

4

5

67

8

910

11

8 12 16 20 24 Min.

Sulfite is a major component of pulping liquor and its analysis isimportant to control corrosion problems. Anion exclusionchromatography provides a simple method for sulfite analysis.

Sulfite inPulping Liquor

Phthalic and isophthalic acids are important to analyze for the saltindustry. They are two monomers that are used in the synthesis ofsalt. They can be analyzed by anion exclusion chromatographywith conductivity detection.

Phthalic, Isophthalic Acids

Acrylic acid is a monomer of polyacrylic acid. The Wescan AnionExclusion Column can separate the monomer and dimer form ofpolyacrylic acid.

Polyacrylic Acid

Analyzing organic acids in samples containing strong acid anionsis possible by anion exclusion chromatography. Strong acid anionsare unretained while weakly ionized organic acids are retained onthe column in the order of increasing pKa.


Ion Chromatography is used to analyze liquors that are used in themanufacturing of printed circuit boards. Electroless nickel solutionis one of the liquors. The identified organic acids can be found inthe bath using anion exclusion chromatography.

Electroless Nickel Solution

6445

8616

6064

6061

5966

Organic Acid Analysis

Organic acids in food and beverages, biological samples, industrialprocess liquors, treated waste waters, and fermentation processesmay be analyzed by anion exchanger (as shown in previouspage) or anion exclusion chromatography. In anion exclusion,highly ionized species are excluded from the pores of the resinand elute in the column void volume. The weakly ionized or non-ionized species are retained on the column and separated. Thistechnique is ideal for the analysis of weakly-ionized organic acidsin the presence of highly-ionized inorganic ions.

Column: Anion Exclusion, 100 x 7.5mm(Part No. 269069)

Mobile Phase: 1mM Sulfuric AcidFlowrate: 0.6mL/minDetector: Conductivity

Column: Anion Exclusion, 300 x 7.8mm (Part No. 269006)Mobile Phase: 3.2mM Nitric AcidFlowrate: 1.2mL/minDetector: Conductivity




Mobile Phase: 0.5mM Benzoic Acid in 10% MethanolFlowrate: 1.0mL/minDetector: Conductivity

Column: Wescan Anion Exclusion , 300 x 7.8mm(Part No. 269006)

Mobile Phase: 1mM Sulfuric AcidFlowrate: 1.0mL/minDetector: UV at 210nm

1. Oxalic acid2. Maleic acid3. Malic acid4. Succinic acid5. Formic acid6. Acetic acid

1. Malic acid2. Lactic acid

1. Monomer2. Dimer

1. Phthalic acid2. Metharylic acid3. Terephthalic acid4. Isophthalic acid

1. Sulfite

1 2

4

5

6

3

0 10 20 Min.

0 5 10 15 20 Min.

1

0 5

1

23 4

10 15 20 25 Min.

12

0 2 4 6 8 Min.

1 2

0 10 20 Min.

App

lica

tion

sOrganic Acids


Trichloroacetic and dichloroacetic acids are important ingredientsin herbicide. Anion exclusion is a reliable method for the analysis ofherbicide.

Herbicides

Ion exclusion columns selectively retain weakly ionized organicacids. A dilute solution of sulfuric or nitric acid is commonly usedas the mobile phase.

Glycolic and Monochloroacetic Acids

Organic acids are added to food products to enhance or modify foodflavor, so they will be more palatable to consumers. They are alsoadded to food products for product quality and stability. WescanAnion Exclusion Column is used for a fast profile of tartaric, lacticand acetic acids in canned foods, like sauerkraut.

Sauerkraut Juice

Haloacetic acids are present in various samples such as drinkingwater, herbicides, antiseptics, and pharmaceutical synthesisproducts. Because many haloacetic acids are toxic, a reliablemethod for their determination is important. Anion exclusionchromatography is a simple method for haloacetic acid analysis.

Haloacetic Acids

Fluoride and acetate, which are byproducts of peptide synthesiscan be analyzed by ion chromatography. Simple dilution and filtrationthrough 0.2µm syringe filter is the only sample preparation requiredfor this application.

Peptide Sample

Anesthetic solution contains sulfite and citrate among other organiccompounds. Wescan Anion Exclusion Column separates sulfiteand citrate rapidly, without the interferences from other ions.Simple dilution with deionized water is the only sample preparationnecessary for this application.

Sulfite and Citric Acid inAnesthetic Solution

6389

6446

6210

6288

6063

8612

Column: Alltech Anion Exclusion, 300 x 7.8mm(Part No. 269006)

Mobile Phase: 1% Phosphoric AcidFlowrate: 0.6mL/minDetector: UV at 210nm

Column: Wescan Anion Exclusion , 300 x 7.8mm(Part No. 269006)



Mobile phase: 3mM Sulfuric AcidFlowrate: 0.5mL/minDetector: Conductivity


Mobile Phase: 1% Phosphoric AcidFlowrate: 0.8mL/minDetector: UV at 210





1. Trichloroacetic Acid2. Dichloroacetic Acid3. Acetic Acid4. Monochloroacetic Acid

1. Trichloroacetic Acid2. Dichlorocaetic Acid

1. Glycolic Acid (50ppm)2. Monochloroacetic Acid (10ppm)

1. Fluoride (10ppm)2. Acetate (100ppm)

1. Citrate2. Sulfite

1. Tartaric Acid2. Lactic Acid3. Acetic Acid

1 2

34

0 11 22 Min. 0 5 10 15 Min.

2

1

1

2

0 5 10 Min.

1

2

3

0 20 Min.5 10 15

Organic AcidsA

pplications

1

2

0 3 6 9 12 Min.

0 5

12

10 15 Min.


Group I and Group II Cations

Carnitine and choline are common vitamin components. Ionchromatography provides a simple method for the analysis of thesecompounds in samples such as vitamins, drugs, and baby foods.

Carnitine and Choline in Vitamins

The Alltech High Speed Cation Column separates lithium, sodium,ammonium, potassium, magnesium and calcium in less than 10minutes by single-column IC.

Rapid and Sensitive Analysis ofGroup I and Group II Cations

Controlling the ion content of power plant water helps preventcorrosion and damage of power plant components. IonChromatography is a fast and accurate method for monitoringcations in power plant water.

Cooling Tower Water

Use the suppressor-based IC for low level cation analysis.Suppressor-based IC provides stable, noise-free baselines,improving detection sensitivity.

Low Level Cations in Reagent Water

The above chromatogram demonstrates the superior resolvingpower of the Universal Cation Column. Samples containing highbackground ionic levels, such as brine and acid digests, can beseparated on the Universal Cation Column with little samplepreparation.

Trace Ammonium in Brine Solution

6291

8455

8457

8089

8462

Cation Analysis

Ion chromatography is a fast and simple method for many typesof cation analyses. Until recently, separating group I and groupII cations in one isocratic run was impossible due to their widelydiffering retentions on sulfonic-acid cation exchangers. Newcarboxylic-acid functionalized cation exchangers, like those usedin the Alltech Universal Cation™ Columns, solve this problem,separating both group I and II cations in one isocratic run. Thecompatibility of these columns with organic and mineral acidmobile phases addresses a wide-range of applications in bothsingle-column and suppressor-based IC modes. The HighSpeed Universal Cation Column separates group I and group IIcations in under 10 minutes. Choose this time-saving column fornon-complex samples. The Universal Cation Column has morecapacity and resolution than the High Speed Universal CationColumn, separating group I and group II cations within 14minutes. Choose the Alltech Universal Cation Column for sampleswith widely varying analyte concentrations, or to separate groupI cations, group II cations, and transition metals in one run.

Column: Universal Cation, 100 x 4.6mm (Part No. 27100)Mobile Phase: 3mM Methanesulfonic AcidFlowrate: 1.0mL/minDetector: Suppressed Conductivity

Column: Universal Cation, 100 x 4.6mm (Part No. 27100)Mobile Phase: 2.5mM Oxalic AcidFlowrate: 1.0mL/minDetector: Conductivity

Column: Universal Cation, 100 x 4.6mm (Part No. 27100)Mobile Phase: 5mM HClFlowrate: 1.0mL/minDetector: Conductivity

Column: High-Speed Universal Cation, 100 x 4.6mm(Part No. 26100)

Mobile Phase: 1.5mM Sulfuric AcidFlowrate: 1.0mL/minDetector: Conductivity

Column: High-Speed Universal Cation, 100 x 4.6mm(Part No. 26100)

Mobile Phase: 1.5mM Sulfuric AcidFlowrate: 1.0mL/minDetector: Conductivity

1. Lithium (10 ppb)2. Sodium (150ppb)3. Ammonium (70ppb)4. Potassium (175ppb)5. Magnesium (50ppb)6. Calcium (50ppb)

1. Sodium (10ppm)2. Ammonium (0.01ppm)3. Potassium (10ppm)4. Magnesium (10ppm)5. Calcium (10ppm)

1. Sodium2. Ammonium3. Magnesium4. Calcium

1. Lithium (0.1ppm)2. Sodium (0.75ppm)3. Ammonium (0.75ppm)4. Potassium (1.25ppm)5. Magnesium (1.0ppm)6. Calcium (1.0ppm)

1. Sodium (1ppm)2. L-Carnitine (3ppm)3. Choline (3ppm)4. Calcium, trace

0 5 10 15 20 Min.

1

2

3 4

56

0 5

1

2

3 4

5

10 15 20 Min.0 2

1

23 4

4 6 8 10 Min.

0 2

1

2

3

4

5

6

4 6 8 10 Min.

1

2 34

0 10 20 30 Min.App

lica

tion

s


Group I and Group II Cations and Lanthanides

Cation determination in oil field water helps to avoid the formationof scale. Scale formation is due to the formation of calcium, bariumand strontium salts. These salts can corrode pipes, tubing and otherequipments. The Alltech Universal Cation Column can separatemany cations in one run.

Cesium and Strontium

Common cations as well as rubidium and barium can be separatedon the Universal Cation column with an acidic mobile phase. Thischromatogram shows the excellent selectivity of the UniversalCation Column as it can separate many different monovalent anddivalent cations in one run.

Rubidium and Barium

Lanthanide analysis is important in mining industries, nuclearpower plants and waste water monitoring. These elements aredifficult to separate because of their similar chemical structures.The Wescan Cation/R, a polystyrene divinyl-benzene based cationexchanger is developed for the separation of lanthanides.

Lanthanides

Morpholine is added to boiler and cooling water to inhibit corrosionand the formation of deposits. Monitoring the levels of this andother volatile amines can reduce the water treatment costs. Cationexchange chromatography is a fast method for morpholine analysis.

Morpholine and Group I & II Cations

Soil composition changes with time. Single-Column IC and UniversalCation Column can provide a simple method for monitoring changesin group 1 and group II cations in soil.

Cations in Soil

Group I and Group II cations in beverages such as coffee and softdrinks, can be easily determined by ion chromatography.

Cations in Coffee Extract

6442

6292

6008

8632

6304

6303

Column: Universal Cation, 100 x 4.6mm (Part No. 27100)Mobile Phase: 3mM Methanesulfonic AcidFlowrate: 1.0ml/minDetector: Conductivity


Column: Universal Cation, 100 x 4.6mm (Part No. 27100)Mobile Phase: 5mM HClFlowrate: 1.0ml/minDetector: Conductivity

Column: Universal Cation, 100 x 4.6mm (Part No. 27100)Mobile Phase: 3mM Methanesulfonic AcidFlowrate: 1.0mL/minDetector: Conductivity


Column: Wescan Cation/R, 100 x 3.2mm (Part No. 269004)Mobile Phase: 1.25mM Ethylenediamine/ 1.67mM Tartaric Acid,

pH 4.2 unadjustedFlowrate: 0.7mL/minDetector: Conductivity

1. Lithium (0.16ppm)2. Sodium (1.2ppm)3. Ammonium (1.2ppm)4. Potassium (2ppm)5. Morpholine (2ppm)6. Magnesium (1.6ppm)7. Calcium (1.6ppm)

1. Lithium (0.12ppm)2. Sodium (0.90ppm)3. Ammonium (0.90ppm)4. Potassium (1.5ppm)5. Magnesium (1.2ppm)6. Cesium (0.8ppm)7. Calcium (1.2ppm)8. Strontium (1.6ppm)

1. Lithium (0.2 ppm)2. Sodium (1.5ppm)3. Ammonium (1.5ppm)4. Potassium (2.5ppm)5. Rubidium (2ppm)6. Magnesium (2ppm)7. Calcium (2ppm)8. Barium (5ppm)

1. Sodium2. Ammonium3. Potassium4. Magnesium5. Calcium

1. Potassium2. Magnesium3. Calcium

1. Ytterbium (20ppm)2. Thulium (20ppm)3. Gadolinium (20ppm)4. Neodymium (20ppm)5. Praseodynium (20ppm)6. Cerium (20ppm)7. Lanthanum (30ppm)

0 4

1

23

4 5 6

7

8 12 16 Min. 0 5 10 15 20 Min.

1 4

6

7

8

53

2

1

2

34 5

0 12 24 Min.

1

23

4

5

6

7 8

0 10 20 30 40 Min.

1

3

2

0 13 26 Min.

76

54

3

21

0 15 30 Min.

Applications


Cations and Transition Metals

Fermentation broths are very important to analyze since thebyproducts of fermentation process may cause feedback inhibition.This method shows the separation of transition metals infermentation broths using the post column derivatization method.

Transition Metalsin Fermentation Broth

Cyanide is used in electroplating, refining precious metals, andmany other chemical processes. Cyanide forms complexes withmetals and they can be separated on the Transition Metal C18Column and detected by UV detection.

Metal-Cyano Complexes

The Universal Cation Column separates transition metals alongwith Group I and Group II cations using a mobile phase containingtwo complexing acids. This method is simpler and less expensivethan the conventional post-column derivatization/UV detectiontechnique.

Monovalent, Divalent andTransition Metals

When analyzing low concentration level of transition metals, postcolumn derivatization with UV detection provides better sensitivitythan conductivity detection. Another advantage of this method isthat common cations will not interfere with the transition metalssince they do not form complexes with the post column reagent.

Transition Metals by PostColumn Reaction

6071

5358

63555357

Column: Universal Cation, 100 x 4.6mm (Part No. 27100)Mobile Phase: 2mM Tartaric Acid/1mM Oxalic AcidFlowrate: 1.0mL/minDetector: Conductivity

1. Copper (1ppm)2. Lead (1ppm)3. Zinc (0.5ppm)4. Nickel (0.5ppm)5. Cobalt (1ppm)6. Cadmium (2ppm)7. Iron (2ppm)8. Manganese (1ppm)

Column: Transition Metal, C18, 250 x 4.6mm(Part No. 269067)

Mobile Phase: 2mM Octane Sulfonate, 40mM Tartaric Acid in5% MeOH, pH 4.1 adjusted with NaOH

Flowrate: 0.8mL/minDetector: Conductivity


Mobile Phase: 0.005M Tetrabutylammonium Sulfate in 30%Acetonitrile

Flowrate: 0.7mL/minDetector: UV at 214nm

Transition Metal Analysis

Transition Metals are traditionally analyzed by cation-exchangeor ion-pair chromatography. A polybutadiene maleic acid coatedsilica-based cation-exchange column (Alltech Universal CationColumn) can be used for transition metal analysis. Using amixture of two complexing acids, several cations including groupI and group II cations along with transition metals can beseparated on this column. Reversed-phase ion interactionchromatography (Ion-pair chromatography) also provides theseparation of divalent transition metals. Both methods can beused with conductivity detection and the detection sensitivity ofthe transition metals are in the high parts-per-billion or in theparts-per-million ranges. For better sensitivity (in the low parts-per-billion range) ion-pair separation along with post column UVdetection method is recommended. Another advantage of thisUV method is that Group I and Group II cations will not interferewith the transition metals if they are present in the sample.

1. Lithium (0.2ppm)2. Sodium (0.5ppm)3. Ammonium (0.5ppm)4. Potassium (0.8ppm)5. Nickel (5ppm)6. Zinc (5ppm)7. Cobalt (5ppm)8. Magnesium (0.7ppm)9. Calcium (0.7ppm)


Mobile Phase: 2mM Sodium Octane Sulfonate/40mMTartaric Acid in 5% MeOH, pH 4.0 adjustedwith NaOH

Flowrate: 1.0mL/minPost Column Reagent: 0.2mM PAR (4-(2-pyridylazo) resorcinol in

3M Ammonium Hydroxide & 1M Acetic AcidReagent Flowrate: 0.5mL/minDetector: UV/Vis at 510nm

1. Fe(CN)64- (5ppm)2. Ag(CN)23- (5ppm)3. Fe(CN)63- (5ppm)4. Ni(CN)42- (5ppm)5. Au(CN)2- (10ppm)

1. Zinc2. Nickel3. Cobalt4. Cadmium5. Iron6. Calcium7. Manganese8. Magnesium

0 25 Min.2015105

65

4

32

1

7

8

9

0 10 20 30 40 Min.

1

2 3 4 5

12 3 4 5 7

68

0 8 16 24 32 Min.

App

lica

tion

s

0 11 22 33 Min.

1

2

3

4

5

6

7

8


Silver is a monovalent cation. It can easily be separated on theUniversal Cation Column with oxalic acid mobile phase. This columnhas excellent resolving power for silver and the other cations.

Analysis of Silver

Aluminum is a trivalent cation and is strongly bound to the ionexchange resin. A high concentration, divalent p-phenylenediaminemobile phase elute aluminum on a Wescan Cation/R Column.

Aluminum

Separation of Group I and Group II cations along with manganese isdifficult with other methods and columns. A pyridine 2, 6-dicarboxylicacid mobile phase alters the selectivity of magnesium and calcium,and separates manganese from magnesium.

Manganese in Soybean Leaves

Separation of trimethyl tin along with other cations is necessary inthe production of trimethyl tin. The Universal Cation Column canseparate these ions in one run in single-column IC mode.

Trimethyl Tin Chloride

Ion chromatography is a simple method for analyzing copper andzinc in brass. A mixture of two complexing acids can elute metalions when using the Universal Cation Column.

Brass Ferrule

Copper, zinc, and lead can be determined with a mixture of tartaricacid/ oxalic acid mobile phase. Simple conductivity detection canbe used for these transition metals as no post column derivatizationreaction with UV detection is required.

Copper, Zinc and Lead

6420

6060

6378

6179

6372

6375

1. Lithium (0.3ppm)2. Sodium (2.07ppm)3. Ammonium (2.07ppm)4. Potassium (4.1ppm)5. Trimethyl tin (20ppm)6. Magnesium (2ppm)7. Iron (10ppm)8. Calcium (2ppm)

Column: Universal Cation, 100 x 4.6mm (Part No. 27100)Mobile Phase: 2mM Tartaric Acid/1mM Oxalic AcidFlowrate: 1.5mL/minDetector: Conductivity

Column: Universal Cation, 100 x 4.6mm (Part No. 27100)Mobile Phase: 3mM Tartaric Acid/2mM Oxalic AcidFlowrate: 1mL/minDetector: Conductivity

Column: Universal Cation, 100 x 4.6mm (Part No. 27100)Mobile Phase: 2.5mM Oxalic AcidFlowrate: 1.0mL/minDetector: Conductivity

Column: Wescan Cation/R, 100 x 3.2mm (Part No. 269004)Mobile Phase: 8.2mM p-Phenylenediamine, pH 3.0 with HClFlowrate: 1.0mL/minDetector: Conductivity

Column: Universal Cation, 100 x 4.6mm (Part No. 27100)Mobile Phase: 3mM Phthalic Acid/

0.5mM Pyridine Dicarboxylic AcidFlowrate: 1.5mL/minDetector: Conductivity

Column: Universal Cation, 100 x 4.6mm (Part No. 27100)Mobile Phase: 5mM Citric AcidFlowrate: 0.8mL/minDetector: Conductivity

1. Lithium (0.1ppm)2. Sodium (3ppm)3. Ammonium (0.7ppm)4. Potassium (1.3ppm)5. Silver (10ppm)6. Magnesium (1ppm)7. Calcium (1ppm)

1. Copper2. Zinc

1. Aluminum

1. Copper (10ppm)2. Sodium (1.2ppm)3. Ammonium (1.2ppm)4. Potassium (2ppm)5. Zinc (10ppm)6. Magnesium (2ppm)7. Calcium (2ppm)8. Lead (10ppm)

1. Sodium2. Potassium3. Manganese4. Unknown5. Calcium6. Magnesium

0 30 Min.252015105

8

7

2

1

3

4 5 6

0 35 Min.30252015105

1

23

4 5 6

1

0 2 4 Min.0 4 8 12 Min.

12

1

23

4

5 6

7 8

0 10 20 30 Min. 0 8 16 24 Min.

76

5

4

3

1

2

Cations and Transition MetalsA

pplications


Cations and AminesA

ppli

cati

ons

Organic cations such as tetrapropyl and tetrabutyl ammoniumcompounds are strongly retained due to the larger ionic sizes.Adding acetronitle to a higher mobile phase concentration canreduce the retention time considerably.

Commercial explosives used in mining and construction industriesuse inorganic salts as inexpensive and effective oxidizing agents.The analysis of these salts is necessary for process control andquality assurance. IC methods replace the traditional long andtedious wet chemical methods.

Oxidizer Salts

Tetrapropyl and Tetrabutyl AmmoniumCompounds

Hydrazine derivatives are used as rocket propellant, corrosioninhibitors, plant growth regulators, antimicrobial drugs and in avariety of agricultural and industrial applications. Due to the toxicnature of these chemicals, it is important to determine them so theywon’t be hazardous to human beings.

Mono and Dimethylhydrazine

Mono-, di-, and tri-ethanolamines are used to scrub carbon dioxideand hydrogen sulfide from refinery water. Ion chromatography isused to monitor ethanolamine concentrations in refinery processstreams and for EPA compliance.

Ethanolamines

The determination of low molecular weight amines in food andbeverages is very important due to the formation of carcinogenicnitrosamines from amines. Also, they may indicate food spoilage.The Universal Cation Column gives a simple method for analyzinglow molecular weight amines.

Alkylamines

The determination of hexamine or hexamethylenetetramine isimportant in commercial explosive manufacturing industries. Thesilica-based Universal Cation Column can separate this compoundfrom other inorganic cations using single-column IC method.

Hexamine

6225

8599

8427

6315

6425

6334

Column: Universal Cation, 100 x 4.6mm (Part No. 27100)Mobile Phase: 5mM Hydrochloric AcidFlowrate: 1.0mL/minDetector: Conductivity

Column: Universal Cation, 100 x 4.6mm (Part No. 27100)Mobile Phase: 3mM Methanesulfonic Acid, 5% AcetonitrileFlowrate: 1.0mL/minDetector: Conductivity

Column: Universal Cation, 100 x 4.6mm (Part No. 27100)Mobile Phase: 3mM Methanesulfonic AcidFlowrate: 1.0mL/minDetector: Conductivity

Column: Universal Cation, 100 x 4.6mm (Part No. 27100)Mobile Phase: 5mM Phthalic AcidFlowrate: 1.0mL/minDetector: Conductivity

Column: Universal Cation, 50 x 4.6mm (Part No. 271092)Mobile Phase: 6.0 Methanesulfonic Acid in 30% AcetonitrileFlowrate: 1.7mL/minDetector: Conductivity

Column: Wescan Cation/R, 100 x 3.2mm (Part No. 269004)Mobile Phase: 3mM Nitric Acid in 10% MethanolFlowrate: 2.0mL/minDetector: Conductivity

1. Monoethanolamine2. Diethanolamine3. Triethanolamine4. Magnesium5. Calcium

1. Ammonium2. Hexamethylenetetramine3. Magnesium4. Calcium

1. Tetrapropylammonium (5ppm)2. Tetrabutylammonium (5ppm)

1. Methylamine (10ppm)2. Dimethylamine (10ppm)3. Trimethylamine (10ppm)4. Calcium5. Triethylamine (10ppm)6. Ethylenediamine (10ppm)

1. Trace Sodium2. Trace Magnesium3. Trace Calcium4. Hexamine

1. Sodium2. Ammonium3. Potassium4. Monomethylhydrazine (16ppm)5. Dimethylhydrazine (19ppm)

1

23

4

5

0 10 20 30 Min.

1

2

3

4

0 14 28 Min.

0 2

1

2

4 6 8 Min.0 15 30 45 Min.

65

4

1 2

3

12 3

4

0 7 14 21 Min. 0 4

1

23

5

4

8 12 16 20 24 Min.


Anionic and Cationic SurfactantsA

pplications

Surfactants may also be separated by ion-pair chromatography ona reversed-phase column with non-suppressed conductivitydetection. This method is however, not as sensitive as thesuppressed conductivity detection method.

Short Chain Cationic Surfactants(Isocratic)

Sodium dodecyl sulfate (SDS) is added to toothpaste as a cleaningagent. Ion chromatography can be used to analyze SDS in toothpastesamples.

Toothpaste

Quaternary alkyl ammonium salts are found in low concentrationsin many cosmetic products. Ion- pair separation with suppressedconductivity is an excellent method for analyzing these surfactants.

Isocratic conditions may be used when all sample surfactants havesimilar hydophobicity (i.e. chain length). Longer-chain surfactantsrequire increased mobile phase organic content.

Alkanesulfonates (Isocratic)

8591

84258429

Cationic surfactants are found in many cosmetic products. Sincemost of these quaternary ammonium compounds are non-chromophoric, suppressed conductivity detection offers a sensitivemethod for this application.

Surfactant Analysis

Surfactants are widely used in commercial detergents, cleansingagents, cosmetics, and hygienic products. Detection of thesecompounds at trace levels is of growing concern due to theireffect on the environment. A reversed-phase separation followedby suppressed conductivity detection provides a successfulmethod for this application.

Column: Surfactant/R Column, 150 x 4.6mm(Part No. 25100)

Mobile Phase: A: 5mM Lithium Hydroxide inAcetonitrile:Methanol:Water (5:5:90)


Column: Surfactant/R Column, 150 x 4.6mm(Part No. 25100)

Mobile Phase: A: 2mM Nonafluoropentanoic AcidB: 100% Acetonitrile

Gradient: Time: 0 5 8 20%B: 30 30 35 80


Column: Alltech Surfactant/R, 150 x 4.6mm (Part No. 25101)Mobile Phase: 2mM Nonapentafluoropentanoic Acid in

30% AcetonitrileFlowrate: 1.0mL/minDetector: Suppressed Conductivity

Column: Surfactant/R Column, 150 x 4.6mm (Part No. 25100)Mobile Phase: A: 10mM Lithium Hydroxide

B: 60:20:20 Acetonitrile:Water:MethanolGradient: Time: 0 3 10 20

%B: 3 3 20 50Flowrate: 1.0mL/minDetector: Suppressed Conductivity

Column: Surfactant C8, 250 x 4.6mm (Part No. 269092)Mobile Phase: 40% Methanol/60% Ammonium Acetate (0.02g/L)Flowrate: 1.2mL/minDetector: Conductivity

1. C7 Sulfonate (10ppm)2. C8 Sulfonate (10ppm)3. C10 Sulfonate (40ppm)

1. Unidentified2. Dodecyl Sulfate (C12)

1. Tetramethyl Ammonium (20ppm)2. Tetrapropyl Ammonium (20ppm)

1. Tetramethyl Ammonium (20ppm) &Tetraethyl Ammonium (20ppm)

2. Tetrapropyl Ammonium (20ppm)3. Tetrabutyl Ammonium (30ppm)4. Dodecyltrimethyl Ammonium (30ppm)5. Cetyltrimethyl Ammonium (40ppm)6. Myristyltrimethyl Ammonium (50ppm)

0 2

2

1

4 6 8 Min.

0 3

3

2

1

6 9 12 15 18 21 Min.0

2

1

4 8 12 16 20 Min.

Alkyl Sulfates5998

1. Octyl Sulfate (C8)2. Decyl Sulfate (C10)3. Dodecyl Sulfate (C12)2

3

1

0 6 12 18 24 30 Min.

Short and Long Chain CationicSurfactants (gradient) 8598

0 5

1

23

45

6

10 15 20 Min.


Trace Anions and CationsA

ppli

cati

ons

8664

Ppt Level Cation Analysis

8709

Ppt Level Anion Analysis

ppb and ppt Level Anion and CationAnalysis

Ion Chromatography has become an important technique for thedetermination of ionic species. Anions and cations in the part-per-million and part-per-billion range can be analyzed easily withdirect injection. For the determination of low levels of ionicimpurities in the low part-per-billion or part-per-trillion levels,sample preconcentration is usually used. Typically, thepreconcentration is accomplished by replacing the injection loopwith a short concentrator column. The sample is loaded using anHPLC pump onto the concentrator column in one direction, thenback-flushed with the mobile phase onto the analytical column,where separation occurs as normal. This technique is limited tosamples that contain low level of interfering ions. This systemalso requires an additional pump in the IC system and is difficultto automate.

Alltech introduces a new instrument (SCAN™ 1000 SampleProcessor) and technique for preconcentration. This newtechnique does not require an additional pump and can easily beautomated with autosamplers. Samples containing highconcentration of interfering ions such as hydroxide and hydrogenions can be neutralized and concentrated at the same time. Thefollowing chromatograms were obtained using the SCAN 1000.

Part per trillion level cations can easily be determined withthe SCAN 1000 Sample Processor. In this application, 5mLof sample was injected. Higher volume samples may beused to analyze lower cation concentrations.

Ultrapure H2O is usually analyzed using the traditionalpreconcentration technique. The SCAN 1000 simplifies theultrapure water analysis. In this sample, chloride andsulfate are detected at less than 1 ppb concentration.

8746

Trace Anions inUltrapure Water

In the power and semiconductor industries, it is important toanalyze ppt level anions and cations. The SCAN 1000 SampleProcessor provides a simple and reliable method for trace-level ion analysis. The SCAN 1000 lets you inject largersample volumes, significantly improving sensitivity.

Column: Universal Cation, 100 x 4.6mmMobile Phase: 3mM Methanesulfonic AcidFlowrate: 1.0mL/minDetector: ConductivitySample Volume: 5mL

1. Lithium (16 ppt)2. Sodium (120ppt)3. Ammonium (205ppt)4. Potassium (200ppt)5. Magnesium (160ppt)6. Calcium (160ppt)

0 4

2

1

3

45 6

8 12 16 Min.

Column: Allsep Anion, 1000 x 4.6mmMobile Phase: 0.85mM NaHCO3/0.9mM Na2CO3

Flowrate: 1.2mL/minDetector: Suppressed ConductivitySample Volume: 4mL

1. Chloride (500ppt)2. Sulfate (500ppt)


0 4

1

2

3

45

6

8 12 16 Min.

Column: Sarasep AN1, 250 x 4.6mmMobile Phase: 1.7mM NaHCO3/1.8mM Na2CO3

Flowrate: 1.0mL/minDetector: Suppressed ConductivitySample Volume: 1.0mL

1. Fluoride (50ppt)2. Chloride3. Nitrite4. Bromide (100ppt)5. Nitrate (100ppt)6. Sulfate (150ppt)

0 4

1

2

8 12 16 Min.

Trace Anions and CationsA

pplications

8640

Cations in Concentrated HCl

This application shows how the instrument can be used asa neutralizer and a concentrator. It is impossible to analyzethe cations in this concentrated acid with direct injection.The SCAN 1000 treats and concentrates the samplesimultaneously. After treating the sample, the hydrochloricacid is neutralized and the low level cations are detectedwith ease.

The SCAN 1000 Sample Processor provides a simple methodto analyze low levels of anions by Single Column IC (SCIC).With 100 to 200 µL direct injection in the SCIC mode, it isdifficult to detect these ppb levels anions. With SCAN 1000,the ppb level anions can easily be analyzed.

8665

Trace Anions by SCIC Method

8706

Trace Cations in Ultrapure Water

Simplify your high purity, trace-level water analysis with theSCAN 1000 Sample Processor. Unlike coventionalpreconcentration techniques, the SCAN 1000 does notrequire a secondary pump and can easily be automated withautosamplers for multi-sample, unattended operation.

SCAN 1000 Sample Processor simplifies the analysis oftrace cationic impurities in various samples. In this leadframe sample, 5mL sample was injected and ppb levelcations are easily quantitated.

8628

Trace Cations in Lead Frame

Column: Universal Cation, 100 x 4.6mmMobile Phase: 3mM Methanesulfonic AcidFlowrate: 1.0mL/minDetector: ConductivitySample Volume: 100µL

Column: Allsep Anion, 100 x 4.6mmMobile Phase: 2.5mM Phthalic Acid, pH 4.2Flowrate: 1.20mL/minDetector: ConductivitySample Volume: 5mL


Column: Universal Cation, 100 x 4.6mmMobile Phase: 3mM Metanesulfonic AcidFlowrate: 1.0mL/minDetector: ConductivitySample Volume: 5mL

1. Sodium (0.5ppm)2. Ammonium (0.3ppm)3. Potassium (0.2ppm)4. Magnesium (0.4ppm)5. Calcium (0.8ppm)

0 3

1

2

3

4 5

6 9 12 15 18 Min.

1. Fluoride (7.5ppb)2. Chloride (15ppb)3. Nitrite (15ppb)4. Bromide (15ppb)5. Nitrate (15ppb)6. Sulfate (22.5ppb)

0 4

1

23 4 5 6

8 12 16 20 Min.

1. Sodium (2.2ppb)2. Potassium (4.0ppb)3. Magnesium (1.1ppb)4. Calcium (1.1ppb)

0 3

1

2

3

4

6 9 12 15 18 Min.

1. Sodium (0.63ppb)2. Ammonium (0.51ppb)3. Calcium (0.43ppb)


0 3

1

2 3

6 9 12 Min.

App

lica

tion

s Acidic, Basic, and Complex Samples

The separation of certain components by ion chromatography can be complicated by the presence of interfering components in thesample matrix. The complication becomes greater when the sample is too acidic, too basic, or one or more components are presentin great excess. The interfering components can overload the analytical column, mask the peaks of interest, or cause baselinedisturbances, thus reducing precision and accuracy. Some interferences may bind irreversibly to the analytical column, causingpermanent damage to the column, thereby reducing column life. Some type of sample preparation is usually necessary to clean-upsamples before analysis.

Alltech offers three products for sample preparation. They are the Sample Concentrator and Neutralizer SCAN™ 1000 SampleProcessor, the Novo-Clean™ IC SPE disks, and the Maxi-Clean™ IC SPE cartridges. With the SCAN 1000, samples are treated on-line as they are analyzed, and are less labor intensive than SPE. With the Novo-Clean and Maxi-Clean devices, samples are treatedoff-line. The following chromatograms show the applications of these products.

Chloride and Nitrate in Caustic



1. Chloride (10ppm)2. Nitrite (10ppm)

0 4 8 12 Min.

1

2

0 4 8 12 Min.

After treating with SCAN 1000,the hydroxide is removed andchloride and nitrate can bequantitated easily. An ERIN*cell packed with cationexchanger in the hydrogenform was used in the SCAN1000 to neutralize thehydroxide.

*Electrochemically Regenerated IonNeutralizer

8481 8482

Complex Matrices

This sample containsapproximately 0.5M sodiumhydroxide. Without the samplepretreatment, the high hydroxideconcentration causes a largenegative peak due to theconversion of the hydroxide towater in the suppressor. Thelarge negative peak interfereswith the anion quantification.

Untreated Treated On-Line w/ SCAN 1000(Part No. 635100 or 635101)

Trace Cations in Concentrated Hydrochloric Acid

Column: Universal Cation, 100 x 4.6mmMobile Phase: 3mM Methanesulfonic AcidFlowrate: 1.0mL/minDetector: Conductivity

1. Sodium (0.5ppm)2. Ammonium (0.3ppm)

After treating the sample withSCAN 1000, the acid isneutralized and themonovalent and divalentcations are easily quantified.An ERIN* cell packed withanion exchanger in thehydroxide form was used inthe SCAN 1000 to neutralizethe acid.

*Electrochemically Regenerated IonNeutralizer

8641 8640

The concentrated hydrochloricacid (12M) was diluted 1:1 withdeionized water. The final acidconcentration in this sample isapproximately 6M. The high acid(hydrogen ion) concentrationcauses a large negative peak,making it impossible to quantitatethe cations in the sample.


3. Potassium (0.2ppm)4. Magnesium (0.4ppm)5. Calcium (0.8ppm)

0 4 8 12 16 Min. 0 4

1

2

3

4 5

8 12 16 20 24 Min.


Trace Cations in Nickel Plating Bath


8489 8490

1. Sodium2. Potassium3. Nickel

0

2

1

3

84 12 16 20 Min. 0 4

32

1

8 12 16 Min.

Complex Matrices

After treating the sample with the SCAN1000, the boric acid is neutralized, allowingfor the quantitation of sodium and potassium.Since nickel hydroxide, which is partiallyinsoluble, is formed after the treatment, nickelin the sample is also removed. The retentiontime for the sodium and potassium returnedto normal. An ERIN cell packed with anionexchanger in the hydroxide form was used inthe SCAN 1000 for this application.

This plating bath solution contains highconcentration of boric acid and nickel.In the untreated sample, the highconcentration of boric acid causes anegative peak that interferes with thesodium peak. The high nickelconcentration produced a large peakon the chromatogram and shifted theretention time of other cations.


Trace Anions in Rock Fusion Sample



8643 8642

1. Fluoride (4.5ppm)2. Chloride (2.5ppm)

After treating the sample with SCAN 1000,the hydroxide is removed and the anions ofinterest can be quantitated easily. An ERINcell packed with cation exchanger in thehydrogen form was used in the SCAN 1000to neutralize the hydroxide.

This sample contains highconcentration of sodium hydroxidesince it was used as the flux materialduring the fusion process. The highhydroxide concentration interferes withthe analysis.


3. Nitrate (1.4ppm)4. Phosphate (723ppm)5. Sulfate (5.0ppm)

Applications

0 63 9 12 15 Min. 0 4

1

2 3

4

5

8 12 16 Min.

Trace Anions in Silica HPLC Stationary Phase8484 8483


Column: Allsep Anion Column, 100 x 4.6mmMobile Phase: 0.85mM NaHCO3/0.9mM Na2CO3


1. Chloride2. Nitrite

By treating the sample with the SCAN 1000,the high hydroxide in silica sample isneutralized and trace anions are separated.

The silica sample was dissolved in0.5M sodium hydroxide solution. Thehigh concentration of sodium hydroxidemasks the trace amount of chlorideand nitrite.

0 5 10 Min. 0 2

1 2

4 6 8 10 Min.


App

lica

tion

s

Anions in Paper Coating (HCl Digest)

Column: Durasep A-2, 100 x 4.6mmMobile Phase: 1.02mM NaHCO3/1.08mM Na2CO3

Flowrate: 1.2mL/minDetector: Suppressed Conductivity w/ SPCS

Anions in Battery Electrolyte (KOH)

Anions in Caustic

Buffer: 5mM Na2CrO4*/0.5mM CTAB,pH 8.0Polarity: NegativeDetector: Indirect UV at 25nmControl Mode: Constant Voltage, 15kV

Anions in a Technical Grade Sodium Carbonate

Untreated Treatedw/Novo-Clean IC-Ag

(Part No. 70266)

6350 6349 Untreated Treatedw/Novo-Clean IC-H

(Part No. 70265)

6406 6407

Untreated Treatedw/Novo-Clean IC-H

(Part No. 70265)

6387 6386Untreated Treated

w/Novo-Clean IC-H(Part No. 70265)

6450 6451

This sample contains highconcentration of chloride. In theuntreated sample, the chloridemasks the nitrate peak, making itimpossible to quantitate.

After treating the sample with Novo-Clean IC-Ag, the nitrate and sulfatepeaks are easily quantitated.

After treating the sample with theNovo-Clean IC-H, the ions of theinterest are easily quantified.Migration times are stable andconsistent.

After treating with Novo-CleanIC-H, the hydroxide is neutralizedand the anions are easilyquantitated.

After treating the sample with Novo-Clean IC-H, trace level of chloride,sulfate, nitrite, nitrate, and phosphatewere found in the sample. Dilution isnot suitable for this application sincethe concentration of the anions isvery low compared to carbonate.

Complex Matrices

1. Chloride2. Nitrate3. Sulfate

1

3

0 13 26 Min.

23

0 26 Min.13The Novo-Clean and Maxi-CleanIC SPE devices may be used forsample treatment before analysisby Capillary Electrophoresis (CE).In the untreated sample, the highhydroxide concentration masks thechloride and sulfate peaks anddecreases their migration times.

1. Chloride2. Sulfate3. Fluoride

This sample contains highconcentration of potassiumhydroxide. High hydroxideconcentration causes a largenegative water dip on a suppressor-based IC, which interferes with thepeaks of interest.

4 5 6 7 Min. 4 5 6 7 Min.

1

2

345

6

7

0 1 2 3 4 5 6 Min.

3

0 1 2 3 4 5 6 Min.

1

2

3

Column: Durasep A-2, 50 x 4.6mmMobile Phase: 1.02mM NaHCO3/1.08mM NaCO3



Buffer: 5mM Na2CrO4*/0.5mM CTAB,pH 8.0Polarity: NegativeDetector: Indirect UV at 25nmControl Mode: Constant Voltage, 15kV

1. Chloride2. Sulfate3. Nitrite4. Nitrate5. Unknown6. Phosphate7. Carbonate

This sample contains 2 grams ofsodium carbonate dissolved in100mL deionized water. In theuntreated sample only carbonatepeak was observed on theelectropherogram.

0 6 12 18 Min. 0 6 12 18 Min.

1

2

3


*US Patent No. 5,128,005, WatersCorporation, Milford MA.

Applications

Anions in Antiseptic Solution

Organic contaminants in thissample cause baselinedisturbances during iodide andsulfate elution, making it difficult toquantitate these anions. Theorganic components may also bindirreversibly to the column packing,slowly damaging the column andreduces its life.

After treating the sample with Maxi-Clean IC-RP, the organiccomtaminants are removed and theanalytes of interest are easilyquantitated. The Maxi-Clean IC-RP removes hydrophobiccomponents from the samplethrough reversed-phase interactionwith the packing.

Anions in Battery Acid

Battery acid contains highconcentration of sulfate. Sulfatein the untreated sample shifts theretention time and interferes withthe quantitation of other anions.

After treating the sample with Maxi-Clean IC-Ba, the sulfateconcentration is reduced and otheranions are easily quantified.

Nitrite in Brine

This sample contains highconcentration of chloride. In theuntreated sample, the chloridemasks the nitrite peak, making itimpossible to quantitate.

After treating the sample with Maxi-Clean IC-Ag, the chloride isremoved and nitrite is easilyquantified.

Monovalent Cations in 1M Nitric Acid

In the untreated sample, the highnitric acid concentration cause thetwo cation peaks to coelute. Alarge negative peak is alsoobserved.

After treating the sample with Maxi-Clean IC-OH, the acid is neutralizedand the two cations are nicelyseparated.

12

3

4

0 5 Min.

0 10 20 Min. 0 10 20 Min.

1

2

0 12 24 Min. 0 12 24 Min.

7654312

7

654

321

0

1

2

0 5 10 15 Min. 5 10 15 Min.

Untreated Treatedw/Maxi-Clean IC-RP

(Part No. 30260)

6251 6250 Untreated Treatedw/Maxi-Clean IC-Ag

(Part No. 30266)

5347 5347

Untreated Treatedw/Maxi-Clean IC-Ba

(Part No. 30266)

5348 5348Untreated Treated

w/Maxi-Clean IC-OH(Part No. 30262)

5352 5352

Complex Matrices

0

12

3

4

5 Min.

Column: Durasep A-2, 50 x 4.6mmMobile Phase: 1.4mM NaHCO3/1.1mM Na2CO3

0.2mM p-CyanophenolFlowrate: 2.0mL/minDetector: Suppressed Conductivity

1. Chloride2. Nitrate3. Iodide4. Sulfate

Column: Anion/R, 250 x 4.1mmMobile Phase: 5mM pHBA, pH 8.5 w/LiOHFlowrate: 1.5mL/minDetector: Conductivity

1. Chloride2. Nitrate

Column: Anion/R, 250 x 4.1mmMobile Phase: 5mM pHBA, pH 8.5 w/LiOHFlowrate: 1.5mL/minDetector: Conductivity

1. Fluoride2. Chloride3. Nitrite4. Bromide5. Nitrate6. Phosphate7. Sulfate

Column: Cation/R, 100 x 3.2mmMobile Phase: 3.2mM HNO3

Flowrate: 1.0mL/minDetector: Conductivity

1. Sodium2. Potassium




Allsep and Allsep A-2 AnionC

olum

ns

Allsep A-2 Anion Columns• Separates Both Weak and Strong Acid Anions in

One Run• Resolves Acetate and Formate Away from

Fluoride and Chloride

Simplify your IC analysis using the Allsep A-2 Anion Column - onecolumn to separate both weakly retained organic and stronglyretained inorganic anions in one run. The Allsep A-2 Anioncolumn separates weakly retained organic anions such as acetateand formate without the interference from fluoride and chloride.The high capacity Allsep A-2 Anion Column is also suitable for theseparation of chlorite and chlorate along with other anions. Sincethis column is hydrophilic in nature, it provides symmetrical peakshape for all anions.

Allsep Anion Columns• Works with Both Suppressor-Based and Single-

Column IC• Separates Anions with Excellent Peak Shape• Resolves Fluoride Away from the Water Dip

Broaden your IC applications with the Allsep Anion Column —the ultimate column for anion separation. This column separatesvarious inorganic and organic anions in both suppressor-basedand single-column IC modes. The hydrophilic characteristics ofthe Allsep Column provide symmetrical peak shape for allanions.

The Allsep Column is compatible with common IC mobilephases: sodium bicarbonate/carbonate, p-hydroxybenzoic acid,phthalic acid, succinic acid, and sodium octane sulfonate.

The Allsep Column is packed with a durable hydrophilic anionexchanger with quarternary amine functionalities. Thehydrophilicity of this packing provides symmetrical peak shapefor all anions especially for polarizable anions such as nitrate.

The Allsep Anion column resolves fluoride away from thewater dip and is an excellent choice for fluoride quantification.Use this column for performing EPA Method 300.0 “Determinationof Inorganic Anions in Water” per US EPA guidelines.

Particle PEEK SSPacking Size Length x ID Part No. Part No. Price

ALLSEP ANION COLUMN

Allsep 7µm 50 x 4.6mm 51214 51213100 x 4.6mm 51207 51200150 x 4.6mm 51209 51208

ALLSEP ANION COLUMNS SPECIFICATIONS

Composition: Methacrylate based w/QuaternaryAmine Functional Group

Particle Size: 7µmMobile Phase Limits: pH 2-11, 0-100% Organic Modifier


ALLSEP A-2 ANION COLUMN

Allsep A-2 7µm 100 x 4.6mm 51219 51218

ALLSEP A-2 ANION COLUMNS SPECIFICATIONS

Composition: Methacrylate based w/AlkanolQuaternary Amine Functional Group

Particle Size: 7µmMobile Phase Limits: pH 2-11, 0-100% Organic Modifier

Introduction to Alltech IC ColumnsAlltech offers a wide range of IC Columns to meet all your ion chromatography needs. The columns are available in stainless steelor PEEK-Lined™ (Metal-Free) formats to use with all IC systems, regardless of manufacturer.

Alltech’s PEEK-Lined Column Systems combine the best mechanical features of stainless steel systems with the biocompatabilityand inertness of PEEK. A threaded PEEK-lined stainless steel tube accepts PEEK inserts, PAT frits and PEEK endfittings. Theentire flow path is biocompatible and metal-free, but maintains the high pressure ratings and good thermal characteristics ofconventional stainless steel columns.

Description Qty Part No. Price

GUARD COLUMNS

All-Guard ™ CartridgesGA-1, 7.5 x 4.1mm (PEEK) 3 38108

Guard HoldersAll-Guard Cartridge Holder Ea 80101

All-Guard Starter KitGA-1 Anion Kit Ea 38109 1 Holder, 3 Cartridges


GUARD COLUMNS






Sarasep AN1 and Anion HCC

olumns

Anion HC Columns• High Capacity for the Separation of 10 Anions in

One Run• Separates Seven Anions Plus Oxyhalides• pH Stable Polymer Media for Longer Column Life

Alltech’s Anion HC column separates 10 inorganic anions requiredby EPA Method 300.0 A and B. The Alltech Anion HC column canseparate seven common inorganic anions plus chlorite, bromate,and chlorate in one isocratic run. This high capacity anionexchange column is packed with poly(styrene-divinylbenzene)with quaternary amine functional group. The column is specificallydeveloped for suppressor-based ion chromatography to performEPA method 300.0 part A and B. The Alltech Anion HC columnis pH stable from 2-12. The large particle size of the packingmaterial allows a higher flowrate of mobile phase for fasterseparations.

ALLTECH ANION HC COLUMN SPECIFICATIONS

Composition: Poly(styrene-divinylebenzene)Quaternary Amine Functional Group

Particle Size: 12µmExchange Capacity: 0.30meq/gEluant Limits: pH 2-12, 0-100% Organic Modifier


GUARD COLUMNS




Particle PEEKPacking Size Length x ID Part No. Price

ALLTECH ANION HC COLUMNS

Anion HC 12µm 100 x 4.6mm 2740012µm 150 x 4.6mm 27410

Sarasep AN1 Columns• Separate Anions with Carbonate/Bicarbonate

and Hydroxide Eluants• Selectivity Comparable to HPIC ™ AS4A Column• Improved Fluoride Determination

The Sarasep AN1 anion-exchange column is compatible withboth suppressor based and single-column ion chromatographysystems. A variety of eluants including sodium carbonate/bicarbonate, sodium hydroxide, and borate/gluconate may beused. Sodium carbonate/bicarbonate eluant combined with thesuppressed conductivity detection allows the separation anddetection of fluoride, chloride, nitrite, bromide, nitrate, phosphate,and sulfate. The selectivity is comparable with HPIC AS4Acolumns. The AN1 columns may also be used for separation ofsulfite and sulfate or selenite or selenate. The unique propertiesof the AN1 resin provide a nice separation of fluoride from thewater dip for a more reliable determination of flouride.

The macroporous polymeric packing is poly(styrene-divinylbenzene) based with alkyl dimethylethanol ammoniumfunctionalities. This material is rugged, and the column bed isstable and exhibits long life. The columns are packed in metal-free hardware that withstands pressures up to 5000psig.

SARASEP AN1 COLUMN SPECIFICATIONS

Composition: Poly(styrene-divinylbenzene)alkyl dimethylethanol ammoniumfunctional group

Particle Size: 8µmExchange Capacity: 0.05meq/gMobile Phase Limits: pH 2-13

Particle PEEKPacking Size Length x ID Part No. Price

SARASEP COLUMNS

AN1 8µm 250 x 4.6mm 38140


GUARD COLUMNS

Sarasep AN1, 50 x 4.6mm, Ea 38145PEEK Guard Column

Replacement Guard-Disc® 5 38146

6316

Separation of Sulfite and Sulfate



1. Water2. Fluoride3. Chloride4. Nitrite

1

23

4

5 6

7

8 9

0 2 4 6 8 10 12 14 16 Min.

5. Bromide6. Nitrate7. Phosphate8. Sulfite9. Sulfate



Wescan Anion/R and Wescan Anion/SC

olum

ns Wescan Anion/R Columns• Quality and Versatility for Anion Determinations• Polymer Support Offers pH Stability• Separates Seven Common Inorganic Anions

The Wescan Anion/R column is packed with poly(styrene-divinylbenzene) based anion exchanger. Because of its high pHstability, this column is ideal for the separation of anions usinghigh pH mobile phases such as p-hydroxybenzoate, sodiumhydroxide, and sodium carbonate/bicarbonate. Thep-hydroxybenzoate mobile phase is best used for the separationof bicarbonate and the seven common inorganic anions bysingle-column IC method. Sodium hydroxide mobile phase isbest used for the separation of weak acid anions such as silicate,borate, sulfide, cyanide, formate, and acetate using single-column IC method with indirect conductivity detection. Thiscolumn can also be used with sodium carbonate/bicarbonatemobile phase for the analysis of sulfite and the seven commoninorganic anions by suppressor-based IC method. The largerdimension columns (150 x 4.6 and the 250 x 4.1mm) are suitablefor a high resolution separation of the seven common inorganicanions. The shorter Anion/R column is better suited for fastseparations.

WESCAN ANION/R COLUMN SPECIFICATIONS

Composition: Poly(styrene-divinylbenzene)Trimethylammonium

Particle Size: 10µm, SphericalExchange Capacity: 0.19 ± 0.02meq/gMobile Phase Limits: pH 2 - 12, 0 - 100% Organic

Modifier


ANION/R COLUMNS

Anion/R 10µm 100 x 4.1mm — 26903110µm 250 x 4.1mm — 26902910µm 100 x 4.6mm 269036 —10µm 150 x 4.6mm 269034 —


GUARD COLUMNS

All-Guard ™ CartridgesAnion/R, 7.5 x 4.6mm (SS) 3 280778GA-1, 7.5 x 4.1mm (PEEK) 3 38108


All-Guard Starter KitsAnion/R (SS) Ea 280779

1 Holder, 3 CartridgesGA-1 (PEEK) Ea 38109

1 Holder, 3 Cartridges

Wescan Anion/S Columns• Analyze Inorganic and Organic Anions• Low Adsorption Characteristics for Rapid Mobile

Phase Equilibration• Hydrophilic Backbone for Sharp, Symmetrical

Polarizable Anions Peaks

The Anion/S columns are best suited for mobile phases in the pHrange from 2 to 5.5. Since fluoride attacks silica, these columnsare not suitable for fluoride analysis. The hydrophilic packingprovides sharp, symmetrical peaks for polarizable anions suchas bromide and nitrate.

Use the 250mm Wescan Anion/S Columns (Part No. 269001and 269011) for routine separations of chloride, bromide, nitrate,and sulfate. Use the shorter Wescan Anion/S Columns (Part No.269013 and 269012) for high-speed separations.

WESCAN ANION/S COLUMN SPECIFICATIONS

Composition: Silica with Quaternary AmmoniumIon Exchanger

Particle Size: 10µmExchange Capacity: 0.25meq/gMobile Phase Limits: pH 2-5.5,

0-100% Organic Modifier


WESCAN ANION/S COLUMNS

Anion/S 10µm 100 x 4.6mm — 26901310µm 250 x 4.6mm — 26900110µm 100 x 4.6mm 269012 —10µm 250 x 4.6mm 269011 —


GUARD COLUMNS

All-Guard ™ CartridgesAnion/S, 7.5 x 4.6mm (SS) 3 12591Anion/S, 7.5 x 4.1mm (PEEK) 3 38133


All-Guard Starter KitsAnion/S (SS) Ea 269090

1 Holder, 3 CartridgesAnion/S (PEEK) Ea 38132




Wescan Anion Exclusion and Universal CationC

olumns

Wescan Anion Exclusion Columns• Separates Organic Acids and Weak Acid Anions• Durable Resin Packing• Rapid Organic Acid Analysis

The Wescan Anion Exclusion Columns are packed with highlysulfonated poly(styrene-divinylbenzene) resin. These columnsseparate organic acids and weakly ionized anions by anionexclusion mechanism. Dilute mineral acids are the typical mobilephases for the separation. Acetonitrile may be used as anorganic modifier to decrease the retention of hydrophobiccompounds.

The longer columns (300 x 7.8mm or 300 x 7.5mm) arerecommended for Krebs Cycle acids and other organic acids.The shorter column is recommended for fast analysis of weakacid anions such as sulfite.

ANION EXCLUSION COLUMN SPECIFICATIONS

Composition: Highly Sulfonated Poly(styrene-divinylbenzene)Cation Exchanger

Particle Size: 10µmExchange Capacity: 1.4meq/gMobile Phase Limits: <40% Acetonitrile,

<15% Isopropyl or Ethyl Alcohols


ANION EXCLUSION COLUMNS

Anion 10µm 100 x 7.8mm — 269068Exclusion 10µm 300 x 7.8mm — 269006

10µm 100 x 7.5mm 269069 —10µm 300 x 7.5mm 269062 —

Description QtyPart No. Price

GUARD COLUMNS

All-Guard ™ CartridgesAnion Exclusion, 7.5 x 4.6mm (SS) 3 269075Anion Exclusion, 7.5 x 4.1mm (PEEK) 3 38118

Guard HoldersAll-Guard Cartridges Ea 80101

All-Guard Starter KitsAnion Exclusion (SS) Ea 269078

1 Holder, 3 CartridgesAnion Exclusion (PEEK) Ea 38117


Universal Cation Columns• Separate Mono and Divalent Cations on Single-

Column or Suppressor-Based IC Systems!• Compatible with Variety of Mobile Phases• Separate Transition Metals without Post Column

Reaction

The Alltech Universal Cation Column1 separates group I andgroup II cations in one run under isocratic conditions. Thiscolumn is compatible with a variety of mobile phases includingcomplexing acids (citric acid, tartaric acid, oxalic acid), mineralacids (nitric acid, hydrochloric acid, sulfuric acid) and non-complexing organic acids (methanesulfonic acid). The AlltechUniversal Cation Column can be used with SCIC or suppressor-based ion chromatography systems.

The High Speed Universal Cation Column separates group Iand group II cations in under 10 minutes (see page 16). Choosethis time-saving column for non-complex samples.

The Universal Cation Column has more capacity and resolutionthan the High Speed Universal Cation Column, separating groupI and group II cations within 14 minutes. Choose the AlltechUniversal Cation Column for samples with widely varying analyteconcentrations, or to separate group I cations, group II cations,and transition metals in one run.

The transition metals are separated using a mobile phasecontaining a mixture of two complexing acids2. This method issimpler and less expensive than the conventional post-columnUV detection method for the transition metal analysis.

1. L.Nair, R.Saari-Nordhaus, and J.M. Anderson, Jr., J. Chromatogr., 640(1993)41-48.2. L. Nair, R.Saari-Nordhaus, and J.M. Anderson, Jr., J. Chromatogr., 671(1994)43-49.

UNIVERSAL CATION COLUMN SPECIFICATIONS

Composition: Spherical Silica Coated withPolybutadiene/Maleic Acid Copolymer

Particle Size: 7µmMobile Phase Limits: pH 2-7, 0-100% Organic


ALLTECH UNIVERSAL CATION COLUMNS

High Speed 7µm 100 x 4.6mm 26101 26100Universal 7µm 100 x 4.6mm 27106 27100Cation


GUARD COLUMNS

All-Guard ™ CartridgesGC-2, 7.5 x 4.6mm 3 271010


All-Guard Starter KitsGC-2 Starter Kit Ea 27109




Cation/R, Scavenger, and Transition MetalC

olum

ns Wescan Cation/R Columns• Durable Resin-based Columns for Cation Analysis• pH Stable from 1 to 12 for Long Column Life• Inert Packing for Use with Organic Modifiers

The Wescan Cation/R Columns rapidly separate monovalent anddivalent cations. Separate lithium, sodium, ammonium, potassium,rubidium, and cesium in one run using a nitric acid mobile phase.Separate divalent cations such as magnesium, calcium, strontiumand barium using ethylenediamine as the mobile phase. Aluminumcan be separated using p-phenylenediamine mobile phase.

The Wescan Cation/R Column may also be used for theseparation of transition metals and Lanthanides. A complexingreagent, such as citric or tartaric acid, is added to the mobilephase to selectively elute the metals off the column.

WESCAN CATION/R COLUMN SPECIFICATIONS

Composition: Poly(styrene-divinylbenzene) withSulfonic Acid Functional Groups

Particle Size: 10µmExchange Capacity: 0.04meq/gMobile Phase Limits: pH 1 - 12, No Acetonitrile, No More

Than 40% Methanol


CATION/R COLUMNS

Cation/R 10µm 50 x 4.6mm 269025 26902410µm 100 x 3.2mm — 269004


GUARD COLUMNS

All-Guard ™ CartridgesCation/R 7.5 x 4.6mm (SS) 3 269083Cation/R 7.5 x 4.1mm (PEEK) 3 38114

Guard HoldersAll-Guard Cartridges Ea 80101

Scavenger Columns• Eliminates Cation and Anion Column Deactivation


CATION SCAVENGER COLUMN

Cation Scavenger Column Ea 382160Anion Scavenger Column Ea 382161

Cation columns are easily “poisoned” by traces of heavy metalions in the mobile phase. Anion columns are easily poisoned bytraces of strongly retained anions, especially when using sodiumhydroxide as the mobile phase. As the column exchange sitesare tied up by the strongly bound ions, retention time decreases.To eliminate this problem, connect the scavenger column betweenthe pump and the injector to remove strongly bound ions from themobile phase. The use of scavenger column eliminates frequentcolumn regeneration and prolongs column life.

Transition Metal Columns• Separate Divalent Transition Metals and Metal-

Cyano Complexes

Divalent Transition Metal Analysis

Separate divalent transition metals by Ion Chromatography.Conductivity detection may be employed for concentrations aslow as 1ppm. For ppb level analysis, a post-column reactionsystem using 4-(2-pyridylazo) resorcinol (PAR) as a derivitizingagent, combined with UV/VIS detection at 520nm is necessary.

Metal-cyano Complexes

Separate metal-cyano complexes using the Alltech Low UV IPCA (Preformulated Tetrabutyl Ammonium Sulfate, 0.005M, PartNo. 185149) as the ion-pair reagent, and methanol or acetonitrileas the organic modifier.

Particle PEEK PistonPacking Size Length x ID Part No. Part No. Price

TRANSITION METAL COLUMNS

C18 3µm 100 x 4.6mm 269064 2288517µm 250 x 4.6mm 269067 228850

Metal-cyano Complexes

TRANSITION METAL COLUMN SPECIFICATIONS

Composition: Silica-based with C18 BondedPhase (20% Carbon)

Particle Size: 7µm, (Standard) Spherical3µm, (High Speed) Spherical

Mobile Phase Limits: pH 2 - 9

1. Fe(CN)64- (5ppm)2. Ag(CN)2- (5ppm)3. Fe(CN)63- (5ppm)4. Ni(CN)42- (5ppm)5. Au(CN)2- (10ppm)

Column: Transition Metal C18, 250 x 4.6mmMobile Phase: 0.005M Tetrabutylammonium Sulfate

(Preformulated Alltech Low UV IPC A,Part No. 185149) in 30% Acetonitrile

Flowrate: 0.7mL/minDetector: UV at 214nm

0 10 20 30 40 Min.

1

2 3 4 5

Description QtyPart No. Price

GUARD COLUMNS

All-Guard ™ CartridgesTransition Metals, 7.5 x 4.1mm (PEEK) 3 38128

Guard HoldersAll-Guard Cartridge Holders Ea 80101

All-Guard Starter KitsTransition Metal (PEEK) Ea 38125


5358



Surfactant and Surfactant/RC

olumns

Surfactant C8, C18 Columns• Separates Short and Long Chain Anionic

Surfactants• Bonded C8 or C18 for a Wide Range of

Applications

Coupling a conductivity detector with an HPLC separationmechanism provides the ideal tool for rapid, sensitive analysis ofanionic surfactants in a variety of matrices. The retention time isreadily controlled by varying the percentage of the organicmodifier (methanol or acetonitrile) in the eluant.

Use the Alltech Surfactant C8 Columns (Part No. 228853,269072 and 269092) for analyzing short chain anionic surfactantssuch as short alkyl sulfates and alkyl sulfonates. For long chainor aromatic surfactants such as xylene sulfonate and dodecylbenzene sulfonate, use the Surfactant C18 Columns (Part No.269071, 228853 and 269091).

Alkyl Sulfates

SURFACTANT COLUMN SPECIFICATIONS

Composition: Silica-based C8 and C18Particle Size: 5µm, SphericalMobile Phase Limits: pH 2-8, 0-100% Organic Modifier

1. Octyl Sulfate2. Decyl Sulfate3. Dodecyl Sulfate

Column: Surfactant C8, 250 x 4.6mmMobile Phase: 0.02g/L Ammonium Acetate in 40% MethanolFlowrate: 1.2mL/minDetector: Conductivity

Particle PEEK PistonPacking Size Length x ID Part No. Part No. Price

SURFACTANT COLUMNS

C18 5µm 250 x 4.6mm 269071 228852C8 5µm 250 x 4.6mm 269072 228853


GUARD COLUMNS

All-Guard ™ CartridgesReversed-Phase, 7.5 x 4.1mm (PEEK) 3 38129


2

3

1

0 6 12 18 24 30 Min.

5998

Surfactant/R Columns• Separates Anionic and Cationic Surfactants• Polymer-based Packing for Extended Durability

The Alltech Surfactant/R Column is designed for the separationof anionic and cationic surfactants by ion-pair chromatographyalong with suppressed conductivity detection. This column mayalso be used for other reversed-phase applications. For anionicsurfactants, lithium hydroxide is used as the ion pair reagent. Forcationic surfactants, the ion-pair reagent is hydrochloric acid.Organic solvents are added to the mobile phase to reduce thereversed-phase interaction between the surfactant and the columnand to modify the selectivity of the separation.

Short and long chain anionic surfactants can be separated inone run by gradient elution. Isocratic conditions can be usedwhen all sample surfactants have similar hydrophobicity (i.echain length).

SURFACTANT/R COLUMN SPECIFICATIONS

Composition: Polydivinylbenzene (DVB) based resinParticle Size: 7µmMobile phase Limits: pH 0-14, 0-100% Organic Modifier

Alkyl Sulfates and Alkanesulfonates

Column: Alltech Surfactant/R, 150 x 4.6mmMobile Phase: A: 10mM Lithium Hydroxide

B: Acetonitrile:Methanol:Water (60:20:20)Gradient: Time : 0 3 10 20

%B: 3 3 20 50Flowrate: 1.0mL/minDetector: Conductivity

1. Sulfate C6 (5ppm)2. Sulfonate C6 (5ppm)3. Sulfonate C7 (10ppm)4. Sulfonate C8 (10ppm)5. Sulfate C8 (20ppm)6. Sulfonate C10 (20ppm)7. Sulfate C10 (30ppm)8. Sulfonate C12 (30ppm)9. Sulfate C12 (40ppm)


SURFACTANT/R COLUMNS

Alltech Surfactant/R Column150 x 4.6mm (SS) Ea 25100150 x 4.6mm (PEEK) Ea 25101

Guard Cartridges for Surfactant/R ColumnsAll-Guard™ GRP-1 Cartridges, 3 381247.5 x 4.1mm

All-Guard Cartridge Holder Ea 80101All-Guard GRP-1 Cartridge Kit Ea 38122 Includes: Holder and 3 Cartridges

0 4

12

3

45

6

7

8

9

8 12 16 20 Min.

8428



Guard ColumnsG

uard

Col

umns All-Guard ™ IC Cartridge System

• Inert Metal-Free Fluid Path• Available in Cost Saving Starter Kits• Simple - Fewer Components• Convenient - Change Cartridges in Seconds

Without Tools• Reliable - Pressure and Chromatographically

Tested

Expensive columns can be a real budget-breaker in the IC lab.Make sure you get the most from your IC columns by protectingthem from contaminants with All-Guard cartridges from Alltech.The All-Guard cartridge system is a break-through in simplicity,reliability and performance. The system has only three sealsurfaces and three parts that makes it the easiest system toassemble. All-Guard’s unique holder is open on the side for fastinstallation of cartridges.

We offer several packing chemistries suitable for use withmost popular IC columns. The 7.5 x 4.6mm cartridges haveadequate capacity to remove contaminants from mobile phase orsample. All-Guard holders and cartridges use uniform, small flowchannels that maintain separation efficiency. Contaminatedcartridges can be replaced in seconds by hand - No tools arerequired.

Starter Kits

For your convenience, Alltech provides Guard Cartridge StarterKits that include an All-Guard Holder and three guard cartridgesall at a substantial savings over individually purchasedcomponents. Replacement cartridges are sold three per package.Connect the All-Guard Holder directly to your analytical columnusing Alltech’s patented Direct-Connect™ Column Coupler,included with each holder. No connecting tubing required.

2763


GUARD COLUMNS

All-Guard ™ Starter Kits (Includes 3 Cartridges, 1 Holder,and 1 Direct-Connect Column Coupler)

GA-1 Anion Ea 38109GC-1 Cation Ea 38113GC-2 Cation Ea 27109GIE-1 Ion Exclusion Ea 38117GRP-1 Resin Reversed-Phase Ea 38122GRP-2 Silica Reversed-Phase Ea 38123

Replacement CartridgesGA-1 Anion 3 38108GC-1 Cation 3 38114GC-2 Universal Cation 3 271010GIE-1 Ion Exclusion Cartridges 3 38118GRP-1 Resin Reversed-Phase 3 38124GRP-2 Silica Reversed-Phase 3 38129

Guard Holder and AccessoriesAll-Guard Cartridge Holder* Ea 80101Replacement Column Coupler Ea 28191

*Includes Direct-Connect Column Coupler.

All-Guard All-Guard Column Starter Kit KitCompany Column Type Type Part No.

GUARD COLUMN REFERENCE TABLE

Dionex IonPac™ AS4, AS4A GA-1 38109 AS4A-SC, AS7, AS9, AS10, AS11, AS12, AS14IonPac™ CS3, CS10, CS11 GC-1 38113IonPac™ CS12, CS14 GC-2 27109IonPac™ NS1 GRP-1 38122IonPac™ AS1, AS5 GIE-1 38117

Waters ™ IC-Pak™-A GA-1 38109IC-PakT-M/D GC-2 27109Ion Exclusion GIE-1 38117µBondapak™ C18 GRP-2 38123Radial-Pak™ C18 GRP-2 38123Nova-Pak™ C18 GRP-2 38123

Hamilton PRP™-X100 GA-1 38109PRP-X200 GC-1 38113PRP-X300 GIE-1 38117PRP-1 GRP-1 38122

Alltech Allsep, Allsep A-2, Anion HC GA-1 38109Wescan Anion/R GA-1 38109Wescan Cation/R GC-1 38113Universal Cation GC-2 271010Wescan Anion Exclusion GIE-1 38117Transition Metal C18 GRP-2 38123Surfactant/R GRP-1 38122Surfactant C8, C18 GRP-2 38123

Misc. Bio-Rad®, Aminex®, HPX-87 GIE-1 38117Interaction® ION-120 GA-1 38109Interaction ION-210 GC-1 38113Interaction ORH-801 GIE-1 38117Metrohm SUPER SEP GC-2 27109



Accessory, Anion, and Cation KitsS

tarter Kits

Alltech IC Accessory and Analysis StarterKits

• Accessory Kit Includes Commonly RequiredFittings, Tubing, and Supplies

• Analysis Kits Include Columns, Standards, andMobile Phases for Common IC Assays

Alltech offers several IC kits to help you set up and maintain yourIC system quickly and economically. The IC Accessory StarterKit (Part No. 269101) is a must for both the new and experiencedIC users. This kit contains metal-free tubing and fittings, a tubingcutter, and other essential supplies for smooth operation ofcommon IC systems — all at a substantial savings over individuallypurchased components.

Alltech’s Anion and Cation Starter Kits contain the columns,guard columns, and mobile phases to perform the most commonanion and cation analyses. Each kit includes an Alltech ICcolumn, matched guard column, EZ-LUTE™ IC Mobile PhaseConcentrate, and IC Standards — all at a substantial savingsover individually purchased components.

Accessories Starter Kit (Part No. 269101)• Accessories Starter Kit Includes Common

Fittings and Tools for IC Systems

Description Part No. Price

ION CHROMATOGRAPHY STARTER KITS

IC Starter KitsAccessories Starter Kit 269101Anion Starter Kit 270102Suppressor-Based Anion Starter Kit 270104Cation Starter Kit 269104Mono and Divalent Cation Starter Kit 269105

Description Qty

ACCESSORIES STARTER KIT

1/16" x 0.007" ID PEEK Tubing (35712) 10ft1/16" x 0.010" ID PEEK Tubing (35702) 10ftPEEK Nuts and Ferrules (35735) 10PEEK 1/16" Union (32141) Ea#22GA Needle with CTFE Luer-Hub (90536) 11mL Plastic Syringe for Injection (44701100) 1220mL Plastic Syringe for Priming Pump (447018) 2Plastic Tubing Cutter (3206) Ea1/4" x 5/16" Open-End Wrench (1998) EaMetal-Free Solvent-Inlet Filter (32170) EaMetal-Free In-Line Filter (68250) Ea

Description Qty

ANION STARTER KIT

Alltech Allsep Anion Column (51207) EaAll-Guard GA-1, Anion Kit (38109) EaEZ-LUTE 4mM pHBA (470200) 6 x 50mLAnion Standards Kit* (37035) Ea/125mL

* 200ppm Each of Certified Fluoride, Chloride, Nitrite, Bromide, Nitrate, Phosphate, and Sulfate

Description Qty

SUPPRESSOR-BASED ANION STARTER KIT

Alltech Allsep Anion Column (51207) EaAll-Guard GA-1 Cartridge Kit (38109) EaEZ-LUTE 0.85mM NaHCO3/ 0.9mM Na2CO3 (470208) EaAnion Standards Kit* (37035) Ea/125mL * 200ppm Each of Certified Fluoride, Chloride, Nitrite, Bromide, Nitrate, Phosphate, and Sulfate

Description Qty

CATION STARTER KIT

Wescan Cation/R Column (269025) EaAll-Guard GC-1 Cation Kit (38113) EaEZ-LUTE 3.2mM Nitric Acid (470111) 6 x 50mLEZ-LUTE 1mM Ethylenediamine 6 x 50mL

pH 6.1 (470112)Cation Standards Kit* (37040) Ea/125mL

* 200ppm Each of Certified Lithium, Sodium, Ammonium, Potassium, Calcium, and Magnesium

Anion Starter Kit (Part No. 270102)• For the Analysis of Anions by Single-Column IC

Technique

Suppressor-Based Anion Starter Kit(Part No. 270104)

• For the Analysis of Anions by Suppressor-basedIC Technique

Description Qty

MONO AND DIVALENT CATION STARTER KIT

Alltech Universal Cation PEEK Column (27106) EaAll-Guard GC-2 Universal Cation Kit (27109) EaEZ-LUTE 3mM Methanesulfonic Acid (470220) 6 x 50mLCation Standard Kit* (37040) Ea/125mL

*200ppm Each of Certified Lithium, Sodium, Ammonium, Potassium, Calcium, and Magnesium

Mono and Divalent Cation Starter Kit(Part No. 269105)

• For the Simultaneous Analysis of Monovalentand Divalent Cations Using the Universal CationColumn

Cation Starter Kit (Part No. 269104)• For the Analysis of Monovalent and Divalent

Cations using the Wescan Cation/R Column



Sta

ndar

dsAnion and Cation Standards

Certified Multi-Component Mixtures

Multi-component mixtures are also sold in 125mL quantities.These mixtures are recommended for QC or method developmentwork.


MULTI-COMPONENT MIXTURES

Anion StandardsMix A: 125mL Mixture Contains: 26910200F(10ppm), Cl(20ppm), NO2(20ppm),Br(20ppm), NO3(20ppm),PO4(30ppm), SO4(30ppm)

Mix 1: 125mL Mixture Contains: 269106F(1ppm), Cl(5ppm), NO2(5ppm),Br(5ppm), NO3(5ppm), PO4(5ppm),SO4(10ppm)

Mix 2: 125mL Mixture Contains: 269107F(1ppm), Cl(10ppm), NO2(10ppm),Br(10ppm), NO3(10ppm),PO4 (10ppm), SO4(10ppm)

Mix 3: 125mL Mixture Contains: 269108F(10ppm), Cl(20ppm),NO2(20ppm), Br(20ppm),NO3(20ppm), PO4(20ppm),SO4(20ppm)

Mix 4: 125mL Mixture Contains: 269109F(20ppm), Cl(40ppm), NO2(40ppm),Br(40ppm), NO3(40ppm),PO4(40ppm), SO4(40ppm)

Mix 5: 125mL Mixture Contains: 269110F(25ppm), Cl(50ppm), NO2(50ppm),Br(50ppm), NO3(50ppm),PO4(50ppm), SO4(50ppm)

Mix 6: 125mL Mixture Contains: 269111Cl(1000ppm), Br(1000ppm),NO3(1000ppm), PO4(1000ppm),SO4(1000ppm)

Mix 7: 125mL Mixture Contains: 269112Cl(15ppm), Br(15ppm), NO3(15ppm),PO4(15ppm), SO4(5ppm)

Mix 8: 125mL Mixture Contains: 269113F(25ppm), Cl(50ppm),SO4(100ppm)

Cation StandardsMix A: 125mL Mixture Contains: 26910300Li(0.5ppm), Na(3ppm), NH4(3ppm),K(6ppm)

Mix B: 125mL Mixture Contains: 26910400Li(0.2ppm), Na(1.5ppm),NH4(1.5ppm), K(2.5ppm),Mg(2.0ppm), Ca(2ppm)

1000ppm 200ppm100mL 100mL

Standard Part No. Price Part No. Price

INDIVIDUAL CERTIFIED IC STANDARDS

NH4 37001 37002Br 37005 37006Ca 37007 37008Cl 37009 37010F 37011 37012Li 37015 37016Mg 37017 37018NO3 37019 37020NO2 37021 37022PO4 37023 37024K 37025 37026Na 37029 37030SO4 37031 37032

1528

Anion and Cation Standards

Add reliability and confidence to your ion chromatographic analysiswith certified analytical standards from Alltech. Our anion andcation standards are prepared from NIST Standard ReferenceMaterials (SRM). Each anion and cation standard is certified byat least two independent methods that are directly traceable toNIST. The method used, the exact concentration, and the NISTSRM number is given on every certificate of analysis. Choosefrom 13 individual standards in 200ppm or 1000ppmconcentrations. Each anion and cation standard is sold in 125mLquantities. All standards are supplied in screw-cap polyethylenebottles. The expiration date is stamped on each bottle.

Certified IC Standards• High Purity Starting Materials• Traceable to NIST• Certified by Two Independent Methods• Convenient Multi-Component Mixtures for QC

and Method Development



Standards and Buffer ConcentratesS

tandards and Buffers

Certified Multi-Standard Kits

Multi-Standard Kits are offered in 200ppm and 1000ppm anionor cation kits - all at a substantial savings over individuallypurchased components.

1527


MULTI-STANDARD KITS

Anion Kits200ppm Kit: Contains 125mL Each of 37035

200ppm Certified Cl, F, Br, NO3, NO2,PO4, & SO4 Standards

1000ppm Kit: Contains 125mL Each of 370361000ppm Certified F, Cl, NO2, Br,NO3, PO4, & SO4 Standards

Cation Kits200ppm Kit: Contains 125mL Each of 37040

200ppm Certified Li, Na, NH4, K, Mg,& Ca Standards

1000ppm Kit: Contains 125mL Each of 370411000ppm Certified NH4, Ca, Li, Mg,K & Na Standards

EZ-LUTE™ Buffer Concentrates• Simplify Mobile Phase Preparation• Pre-formulated Buffers for Accurate

Concentrations Every Time• Wide Selection for Anion, Cation, and Organic

Acid Analyses• Single-Column and Suppressor-Based IC Buffers

For the most consistent results, mobile phases should be preparedfresh daily. This minimizes the potential for changes in pH andconcentration caused by bacterial degradation or atmosphericabsorption. Unfortunately, weighing and dilution can be time-consuming (and therefore expensive) procedures. As a result,there is a common tendency to “get by” and stretch a batch ofbuffer out for a few days or a week. All too often, the result is “oldbuffer syndrome”, a significant deterioration in reproducibility,efficiency, and column lifetime.

Alltech has a sure cure for “old buffer syndrome” - EZ-LUTEconcentrates. These buffer concentrates are so easy andeconomical to use. Simply dilute the EZ-LUTE concentrate withdegassed, deionized water to make up your mobile phase bufferaccurately every time! Each bottle of SCIC buffer makes 2 litersof mobile phase. Each bottle of bicarbonate/carbonate buffermakes 10 liters of mobile phase.


EZ-LUTE BUFFER CONCENTRATES

SCIC Buffers, 6 x 50mL4mM LiHP, pH 4.5 4701041mM pHBA 4702042.5mM pHBA 4702064mM pHBA 4702005mM pHBA 4701057mM pHBA 4701065mM LiOH/Benzoate 4701073.2mM Nitric Acid 4701111mM EDAC, pH 6.1 4701125mM Citric Acid 4701177mM Citric Acid 4701181mM Sulfuric Acid 4701104mM Phthalic Acid 4701023mM Methanesulfonic Acid 470220

SCIC Buffers, 6 x 100mL20mM Phthalic Acid 470101

Suppressor-Based IC Buffers, 1 x 100mL1.7mM NaHCO3/1.8mM Na2CO3 4701192.8mM NaHCO3/2.2mM Na2CO3 4702010.68mM NaHCO3/0.72mM Na2CO3 4702022.1mM NaHCO3/1.6mM Na2CO3 4702031.02mM NaHCO3/1.08mM Na2CO3 4702051.19mM NaHCO3/1.26mM Na2CO3 4702070.85mM NaHCO3/0.9mM Na2CO3 470208500mM NaHCO3 470209500mM Na2CO3 470210


Organic acid standards are available in 125mL quantities at1000ppm concentrations. They are prepared from 90 - 95% purechemicals and are not traceable to NIST. These standards arerecommended for QC and methods development work.


ORGANIC ACID STANDARDS

Organic Acid StandardsOxalic Acid, 1000ppm 37033Maleic Acid, 1000ppm 37037Malic Acid, 1000ppm 37039Succinic Acid, 1000ppm 37043Formic Acid, 1000ppm 37045Acetic Acid, 1000ppm 37047Citric Acid, 1000ppm 37049Tartaric Acid, 1000ppm 37051Lactic Acid, 1000ppm 37053Abietic Acid, 1000ppm 37055Methane Sulfonic Acid, 1000ppm 37057



Acc

esso

ries

Metal-Free Tubing and Tubing Cutter

0750

Flex-Connect ™ Tubing• Self-Adjusting Length• Will Not Bend or Kink• Made of PEEK –

Biocompatible andChemically Inert

• Fittings Included

Flex-Connect™ Tubing from Alltech is the most convenient wayto connect components on your HPLC or IC system. The coiledformat provides flexibility for easy installation and springs back tokeep excess tubing out of your way. Each piece comes with 6"of straight tubing on each end for connections as well as twoWrench-Fingertight Fittings and Double-Sided Ferrules for areliable connection to any standard 10-32 port.

Flex-Connect is the ideal choice for modular LC and ICsystems, giving you the freedom to move components withoutbreaking connections.

Teflon Tubing• Chemically Inert• Easily Cut and Flanged

Dimensions Qty Part No. Price

TEFLON TUBING

TFE Tubing1/16" OD x 0.007" ID 10ft 356771/16" OD x 0.010" ID 10ft 200641/16" OD x 0.020" ID 10ft 200331/16" OD x 0.030" ID 10ft 200311/16" OD x 0.040" ID 10ft 201061/8" OD x 1/16" ID 10ft 200631/8" OD x 0.100" ID 10ft 20096

Color-Coded PEEKTubing

• Easy to Cut with RazorBlade or Alltech TubingCutter

• High Strength PolymerReplaces Stainless Steel

• Chemically Inert andBiocompatible

PEEK (Poly Ether Ether Ketone) tubing is the ideal metal-freetubing for high pressure applications. Its mechanical strength,chemical resistance and biocompatibility are properties thatmake it appropriate for a wide variety of LC and IC applications,especially those requiring metal-free conditions.

1036

Solid or Striped Coding for Easy Identification of Internal Diameter.

Tubing Max. Striped SolidColor OD x ID (psig) Qty Part No. Part No. Price

COLOR CODED PEEK TUBING

Red 1/16" x 0.005" 8,000 10ft 35714 35720Yellow 1/16" x 0.007" 8,000 10ft 35712 35722Blue 1/16" x 0.010" 6,000 10ft 35702 35728Orange 1/16" x 0.020" 6,000 10ft 35708 35726Green 1/16" x 0.030" 5,000 10ft 35710 35724Grey 1/16" x 0.040" 3,000 10ft 35705 —

* First dimension is relaxed coil, second dimension is extended coil. Does notinclude straight lengths at ends.

Tubing Pres.Rate Size Striped SolidColor ID (psig) Range* Part No. Part No. Price

FLEX-CONNECT PEEK TUBING

Red 0.005" 8,000 1-5cm 35851 358010.005" 8,000 2-10cm 35852 358020.005" 8,000 3-15cm 35853 358030.005" 8,000 5-25cm 35854 35804

Yellow 0.007" 8,000 1-5cm 35861 358110.007" 8,000 2-10cm 35862 358120.007" 8,000 3-15cm 35863 358130.007" 8,000 5-25cm 35864 35814

Blue 0.010" 6,000 1-5cm 35871 358210.010" 6,000 2-10cm 35872 358220.010" 6,000 3-15cm 35873 358230.010" 6,000 5-25cm 35874 358240.010" 6,000 10-50cm 35875 358250.010" 6,000 15-75cm 35876 35826

Orange 0.020" 5,000 1-5cm 35881 358310.020" 5,000 2-10cm 35882 358320.020" 5,000 3-15cm 35883 358330.020" 5,000 5-25cm 35884 35834

Green 0.030" 4,000 1-5cm 35891 358410.030" 4,000 2-10cm 35892 358420.030" 4,000 3-15cm 35893 358430.030" 4,000 5-25cm 35894 35844


PLASTIC TUBING CUTTER

Plastic Tubing Cutter 3206Replacement Blade 3204

This is a spring-loaded cutter forplastic tubing. The hardenedsteel blade produces clean,right-angle cuts. Compact – only3" long!

Plastic Tubing Cutter

0090



High Pressure Metal-Free FittingsA

ccessories

Metal-Free Unions, Tees and Crosses• Universal• Chemically Inert• Twice the Gripping Power

Graphic Description Material Qty Part No. Price

METAL-FREE UNIONS, TEES AND CROSSES

Metal-Free Unions, Tees and CrossesA Union PEEK Ea 32141B Tee PEEK Ea 32143C Cross PEEK Ea 32145Bulkhead UnionD 10-32 PEEK Ea 31504

A

No-Slip One-Piece Fittings• No-Slip ‘O’-Ring Keeps Fitting on the Tubing

Between Connections• Fits All Conventional 10-32 Ports• Chemically-Inert, PEEK Construction• Convenient, Built-In Ferrule

Tired of searching the bench or floor looking for a fitting that felloff the tubing? Alltech’s new No-Slip fittings stay in placebetween connections because a built-in ‘o’-ring lightly grips thetubing even when the fitting is not connected to a mating port.This exclusive feature keeps the fitting in place when you’rechanging columns, sample loops, or any other time the fitting isnot engaged. The non-wetted ‘o’-ring will not compromisechemical resistance.

The No-Slip One-Piece fitting has a unified nut and ferrule.The built-in ferrule eliminates the inconvenience of additionalloose parts, while still including all the essentials for a leak-freeseal.

One-Piece Fingertight Fittings• Fingertight – One-Piece Construction• Universal – Works With All 1/16" OD Tubing and

Female 10-32 Ports• 100% PEEK Construction – Inert & Biocompatible

Graphic Description Mat'l Qty Part No. Price

NO-SLIP ONE-PIECE FITTINGS

No-Slip One-Piece FittingsA Short Finger-Tight PEEK 10 32293B Long Finger-Tight PEEK 10 32295C Short Hex Head PEEK 10 32297D Long Hex Head PEEK 10 32299

Graphic Color Mat'l Qty Part No. Price

ONE-PIECE FINGERTIGHT FITTINGS

One-Piece Fingertight FittingsA Natural PEEK 10 32233

Double-Sided Ferrule (DSF) Fittings• Universal• Chemically Inert• Twice the Gripping Power• Suitable For Use with All 10-32 Threaded Ports

and Fittings

These fittings use a Double-Sided Ferrule. In addition to beingUniversal, the double-sided design is ideal for polymeric tubing.

By gripping the tubing in two places, the DSF has increasedholding power without concentrating all the force on one area ofthe tubing. This makes them perfect for use with polymeric tubingsuch as PEEK or Tefzel®, which may be crushed by other fittingsystems when tightened for high-pressure applications.

A

Graphic Color Material Qty Part No. Price

DOUBLE-SIDED FERRULE FITTINGS

Standard Wrench/Fingertight Nuts with FerrulesA Natural PEEK 10 35753

Male-Female Wrench/Fingertight Nuts with FerrulesB Natural PEEK 10 35755

Long Hex-Head Nuts with FerrulesC Natural PEEK 10 35735

Short Hex-Head Nuts with FerrulesD Natural PEEK 10 35744

Replacement FerrulesE Ferrules PEEK 10 35740E Ferrules Kel-F® 5 32025

B C D

A B C D

D E

A B C



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Low Pressure and Flange-Free Plus Metal-Free Fittings

Flange-Free ™ Fittings• Compatible with all 1/4-28 TPI Fitting Systems• Connects Directly to any 1/16" or 1/8" Tubing• Connects Finger Tight—No Tools Required

The Flange-Free System makes flanged tubing connections obsolete(Figure 1 ). The connection (Figure 2 ) can be made quickly and easily with noflanging tools. Hand-tightened connections produce a leak-free seal to 500psigon 1/8" OD Teflon® tubing and to 1,000psig on 1/16" OD Teflon tubing.

Figure 1

Tees, Crosses and Adaptors for Flanged or Flange-Free Fittings

The fittings listed on this page all feature 1/4-28 TPI threaded ports and will work with Flange-Free or conventional flanged fittings.

1/16" OD 1/8" ODTubing Tubing

Graphic Description Material Qty Part No. Part No. Price

FLANGE-FREE NUTS AND FERRULES

A PEEK NutsNatural PEEK 5 20124 20116

B Ferrules1/16" Bore PEEK 5 20117 —1/8" Bore PEEK 5 — 201271/16" Bore Kel-F® 5 20115 200871/8" Bore Kel-F 5 — 20125

* U.S. Patent #4690437.

Graphic Description Material Qty Part No. Price

1/4-28 LOW PRESSURE FITTINGS

UnionsPEEK Union A PEEK Ea 20088Bulkhead B PEEK Ea 31506

Tees0.8mm Bore Tee C PEEK Ea 200581.5mm Bore Tee C PEEK Ea 20023

Crosses0.8mm Bore Cross D PEEK Ea 200591.5mm Bore Cross D PEEK Ea 20025

Adaptors1/4-28 to Male Luer Adaptor E Tefzel® Ea 200191/4-28 to Female Luer Adaptor F Tefzel Ea 200551/4-28 to 1/16"-5/32" ID Flexible Tubing Adaptor G PEEK Ea 28848Female 1/4-28 to Female 10-32 Adaptor H PEEK 2 32262Male 1/4-28 to Female 10-32 Adaptor I PEEK 2 32261

Luer Adaptors10-32 Female to Female Luer J PEEK Ea 201321/4-28 Female to Female Luer J PEEK Ea 20136M6 Female to Female Luer J PEEK Ea 2013810-32 Female to Male Luer K PEEK Ea 201331/4-28 Female to Male Luer K PEEK Ea 20137M6 Female to Male Luer K PEEK Ea 20139

D

B

C

E

F

��G

K ��

H

J ��

I

Figure 2

Improved Pressure Rating withAlltech’s Flange-Free Plus ™

• Seals Fingertight to 5,000psig• Connects to any Standard 1/4-28 Port

The Flange-Free Plus fitting system improves thepressure rating for connections to a standard 1/4-28Port. A simple, stainless-steel bushing helpscompress the ferrule against the tubing for a secureseal to 5,000psig. And since the fitting doesn’t bindto the bushing, connections are made easily, withouttwisting your tubing.

The Flange-Free Plus system uses a PEEKferrule for its liquid seal, maintaining an inert, metal-free fluid path. That PEEK ferrule is held securelyin place by the mechanical strength of the stainless-steel bushing.


FLANGE-FREE PLUS FITTINGS

Fitting System 10 25020 Includes Fittings,

Bushings, andFerrules

Replacement PartsBushings 10 25022Ferrules 10 25021

A



Injection Valves, Sample Loops, Syringes, and FiltersA

ccessories

Model 9725 and 9725i Dual Mode PEEKInjection Valves

• Accommodate Complete or Partial Loop Filling• 5000psig Maximum Pressure

The 9725 and 9725i are six-port rotary Dual Mode Injection valve.The needle port design permits sample introduction by eithercomplete or partial loop filling. Since the wetted materials arePEEK and Tefzel, these valves are ideal for applications requiringa biocompatible or metal-free flow path. Also available is theSuction Needle Adaptor which eliminates exposure of the sampleto the metal of a syringe needle. The 9725i comes with built-inposition sensing switch.


PEEK INJECTION VALVES

Model 9725 40010Model 9725i w/ Position Sensing Switch 40011Suction Needle Adaptor 40016

Flex-Connect ™ Sample Loops• Flexible Coil Bends into Place

Quickly and Easily• Universal Fittings Prevent

Dead Volume• 100% PEEK Construction –

Prevents Corrosion andSample Loss

Flex-Connect Sample Loops are the most convenient sampleloops to use with your Rheodyne injection valve. The loop coilexpands and contracts to make alignment and installation of theloop quick and convenient.

Each Flex-Connect Sample Loop is supplied with Hex-HeadNuts and a special two-piece ferrule. This new ferrule allows youto tighten the fitting without twisting the tubing.

0813


ALLTECH FLEX-CONNECT SAMPLE LOOPS

Flex-Connect Sample Loops5µL Flex-Loop Ea 3219410µL Flex-Loop Ea 3219620µL Flex-Loop Ea 3219550µL Flex-Loop Ea 32202100µL Flex-Loop Ea 32204200µL Flex-Loop Ea 32206500µL Flex-Loop Ea 322101mL Flex-Loop Ea 32222

FittingsShort Hex-Head Nut and Ferrule 5 32215Long Hex-Head Nut and Ferrule 5 32217

Two-Piece Ferrule 5 32218

Plastic Syringes• Inexpensive• Free of Contaminant Ions• Non-Breakable

Plastic syringes with Luer tip are ideal for injection of aqueoussamples in ion chromatography. They are inexpensive and non-breakable. Use of plastic syringes eliminates the possibleleaching of contaminant ions that are often associated with glasssyringes.


PLASTIC SYRINGES

1mL Plastic Syringes 12 447011005mL Plastic Syringes 12 44701220mL Plastic Syringes 2 447018#22 GA Needle w/CTFE Luer-hub 6/pkg 90536

1224

Metal-Free Pump Inlet Filter• Replaceable 20 µm Porosity Filter Elements• Threaded Holder allows Easy Connection

Alltech’s solvent in-line filter removes damaging particulates inthe mobile phase before they enter the HPLC system. The filterconsists of a reusable PEEK port, nut and bushing and aninexpensive replacement filter element.

��

0735

Metal-Free In-Line Filter• Extend Column Life• Reduce Equipment Wear• Economical Replacement Filter Elements

Alltech’s high-pressure in-line filter removes damaging particulateswithout exposing samples or mobile phases to metal surfaces.This In-Line filter is placed between the injection valve andcolumn and consists of a reusable PEEK holder and inexpensivePAT™ (PEEK Alloyed with Teflon®) replacement filter elements.

0729

Porous Polypropylene Filter Pump Inlet Tubing

PEEK Nut and BushingFemale 1/4"-28 PEEK Port


PUMP-INLET FILTERS

Alltech Metal-Free Pump Inlet FilterFilter with 1/8" Fitting Ea 32170Filter with 1/16" Fitting Ea 32171Replacement Filter Elements 5 32173

Alltech Metal-Free In-Line FilterMetal-Free In-Line Filter with Ea 68250 2µm Filter ElementReplacement 2µm Filter Elements 5 68152


Sam

ple

Pre

para

tion

Maxi-Clean IC

For Maxi-Clean application chromatograms,refer to pages 27.

See page27

The Maxi-Clean IC-Ag and IC-Ba depend on selectiveprecipitation reactions to remove or reduce the concentration ofhalides or sulfate, respectively. When a large concentration ofhalides or sulfate is present in a sample, low levels of otheranions may be lost during pretreatment with the Maxi-CleanIC-Ag or IC-Ba[2]. A study of the recovery of ions of interest ineach sample matrix should be determined before quantitativeresults are obtained.

The Maxi-Clean IC-Na can be used to remove cations fromsamples without changing the pH of the sample. The Maxi-CleanIC-Chelate removes polyvalent cations and transition metalsfrom the sample. The Maxi-Clean IC Mixed-Mode RP-OHCartridge provides simultaneous removal of hydrophobiccomponents and increases the pH of acidic samples. The Maxi-Clean IC Mixed-Mode RP-H Cartridge provides simultaneousremoval of hydrophobic components and decreases the pHof alkaline samples.[1] R. Saari-Nordhaus, J.M. Anderson, Jr. and I.K. Henderson, Am. Lab. August

(1990) 18.[2] I.K. Henderson, R. Saari-Nordhaus and J.M. Anderson, Jr., J. Chromatogr.

546 (1991) 61.


MAXI-CLEAN IC

Maxi-Clean IC Cartridges, 0.5mL Bed VolumeIC-RP 50 30260IC-OH 50 30262IC-H 50 30264IC-Ag 50 30266IC-Ba 50 30268IC-Na 50 30270IC-Chelate 50 30250

Maxi-Clean IC Plus Cartridges, 1.5mL Bed VolumeIC-RP Plus 25 30252IC-OH Plus 25 30254IC-H Plus 25 30256IC-Ag Plus 25 30258IC-Ba Plus 25 30261IC-Na Plus 25 30263IC-Chelate Plus 25 30265

Maxi-Clean IC Mixed Mode Cartridges, 0.5mL Bed VolumeMixed-Mode RP-OH 25 30259Mixed-Mode RP-H 25 30267

Eliminates Matrix Interferences BeforeIon Analysis

1545

The most difficult part of many ion chromatography applicationsis eliminating interfering components from the sample matrix.These interferences may co-elute or mask peaks of interest,overload the column, or bind irreversibly to the column packingthereby shortening its useful life. Maxi-Clean IC cartridgeseliminate many of these interferences[1,2].

Each cartridge contains 0.5mL (Maxi-Clean™ IC) or 1.5mL(Maxi-Clean IC Plus) of purified polystyrene resin, containedby 20µm polyethylene frits within an injection-moldedpolypropylene housing. The resin has been functionalized toretain specific types of components from the sample. Thecartridges have luer inlets and outlets allowing samples to beprocessed with any luer-hub syringe.

Maxi-Clean IC Cartridges are available in nine differentchemistries to address a variety of IC sample preparationproblems. The Maxi-Clean IC-RP provides a reliable method forthe removal of surfactants, organic acids, proteins and otherorganic substances. The Maxi-Clean IC-H and IC-OH adjust thepH of acidic or alkaline sample prior to injection. The recovery ofweakly ionized anions such as nitrite and phosphate may bepoor after sample pretreatment with the Maxi-Clean IC-H[2].

Extract-Clean™ IC

Designed for labs with higher samplethroughput requirements, Extract-Clean ICtubes contain the same 0.5 or 1.5mL ofpolystyrene resins as the original Maxi-CleanIC. The standard 3.0mL Extract-Clean tube isterminated in a male luer tip that can be usedwith a vacuum manifold or with a lab robot likethe Gilson ASPEC or Zymark Benchmate™.

Successful use of Extract-Clean IC dependson the same parameters as the Maxi-CleanIC: 1) proper tube conditioning, 2) sampleflowrates of 1mL/min or less, and 3) controlof the sample size to stay within the tube’scapacity.

0368


EXTRACT-CLEAN IC

Extract-Clean IC Cartridges, 0.5mL Bed VolumeIC-RP 50 40260IC-OH 50 40262IC-H 50 40264IC-Ag 50 105050IC-Ba 50 40268IC-Na 50 40270IC-Chelate 50 40250

Extract-Clean IC Cartridges, 1.5mL Bed VolumeIC-RP Plus 3 30 140252IC-OH 30 140254IC-H 30 140256IC-Ag 30 140258IC-Ba 30 140261IC-Na 30 140263IC-Chelate 30 140265


Maxi-Clean ICS

ample P

reparation

Trace Ions in AnilineProcedure

1. Extract 1g of Aniline with a mixture of methylenechloride and 1% sodium hydroxide (5mL each).

2. Apply 4mL of aqueous layer from step 1 to cartridge.

3. Discard the first 1mL of eluate.

4. Collect and analyze remaining eluate.

Unextracted Extracted

Column: Anion/S, 250 x 4.6mmMobile Phase: 4mM KHP, pH 4.5, w/LiOHFlowrate: 2mL/minDetector: Conductivity


Capacity (Meq)Cartridge Packing Retains 0.5mL/1.5mL Applications

MAXI- AND EXTRACT CLEAN IC

IC-RP Polystyrene Hydrophobic Variable Removes surfactants, organic acids, and other organiccomponents substances. Inorganic ions pass through.

IC-OH Strong anion exchanger Anions 0.6 / 1.5 Exchanges anions for hydroxide. May be used toin hydroxide form remove or concentrate anions from sample and to

increase pH of acidic samples. Removes cations thatform insoluble hydroxide salts.

IC-H Strong cation exchanger Cations 0.8 / 2.0 Exchanges cations for H+. May be used to remove orin H+ form concentrate cations from sample and to reduce pH of

basic samples.IC-Ag Strong cation exchanger Chloride 0.8 / 2.0 Removes excess halides through formation of

in Ag+ form Iodide Ag-halide salts.Bromide

IC-Ba Strong cation exchanger Sulfate 0.8 / 2.0 Removes excess sulfate through formation of BaSO4.in Ba+2 form

IC-Na Strong cation exchanger Cations 0.8 / 2.0 Exchanges cations for Na+. May be used to removein Na+ form or retain cations from sample without changing the pH

of the sample.IC-Chelate Styrene-divinylbenzene Polyvalent 0.2 / 0.5 Exchanges transition metals and divalent cations for

containing chelating group metal ions Na+. May be used to remove or retain divalent cations(iminodiacetate in Na+ form) and transition metals from sample.

IC Mixed- Polystyrene/strong anion Hydrophobic Variable- Simultaneously removes hydrophobic components andMode exchanger in hydroxide components 0.6 anions and increases the pH of acidic samples.RP-OH form and anionsIC Mixed- Polystyrene/strong cation Hydrophobic Variable- Simultaneously removes hydrophobic components andMode exchanger in hydrogen components 0.8 cations and reduces the pH of basic samples.RP-H form and cations

0 12 24 Min. 0 12 24 Min.

32

1

Anions in HCl

Column: Durasep A-2, 100 x 4.6mmMobile Phase: 1.02mm NaHCO3/1.08mm Na2CO3


1. Fluoride2. Formate3. Chloride

Unextracted Extracted

0 5 10 15 20 25 Min. 0 5 10 15 20 25 30 Min.

12 4

5

6

3

8351

4. Bromide5. Nitrate6. Sulfate

Maxi-Clean IC Mixed-Mode RP-H Removes OrganicContaminants and Neutralizes Hydroxide

Maxi-Clean IC-Ag Removes Chloride

5351


Sam

ple

Pre

para

tion

Novo-Clean IC

3129

Need more information onNovo-Clean IC?

Request Bulletin #364

For Novo-Clean application chromatograms,refer to page 26.

See page26

Remove IC and CE Matrix Interferenceswith Novo-Clean™ IC Solid-PhaseExtraction Disks

• Revolutionary Impregnated PTFE-MembraneEliminates Bed Channeling

• High Cross-Sectional Area Increases ExtractionSpeed

• High-Purity Membrane and Housing EliminateIonic Contaminants

• Convenient Disposable Disks are Affordable andEasy to Use

Novo-Clean IC Disks are useful for sample clean-up before IC orCE[1,2]. The disk contains high purity polystyrene-divinylbenzenecation exchange beads impregnated in a PTFE membrane,contained in a polypropylene housing. Simply pass your samplethrough the membrane using a luer-hub syringe or vacuummanifold. As the sample passes through the membrane, specificion-exchange interactions selectively retain matrix interferenceswhile analytes pass through unchanged. Collect the Novo-Cleaneffluent and inject!

Novo-Clean SPE Disks replace packed-bed SPE devices witha wide, thin section of membrane. This uniform, stable extractionmatrix eliminates bed channeling that is often encountered withpacked-bed devices, increasing capacity and precision. Samplesare easier to push through the disks because of the high crosssectional area.

*Total capacity measurement is based on Novo-Clean IC-H. It may vary slightly with Ag form.

Typical CapacityDisk Membrane Retains 25mm/13mm Applications

NOVO-CLEAN™ IC DISK SPECIFICATIONS

IC-H Strong Cation Exchanger Cations 2.0meq/0.5meq Exchanges cations for hydrogen.in the hydrogen form. Removes or concentrates sample cations or

reduces pH of basic samples.

IC-Ag Strong Cation Exchanger Chloride 2.0meq* Exchanges cations for silver.in the silver form. Iodide Removes excess halides through formation

Bromide of silver-halide salts.

1076

Process samples with a syringe or a vacuum manifold.Loose 13mm or 25mm Novo-Clean membranes are also

available to fit standard filter holders.

PartDescription Qty No. Price

ALLTECH NOVO-CLEAN IC DISKS

Novo-Clean Disks13mm Novo-Clean IC-H Disk 50 7026425mm Novo-Clean IC-H Disk 25 7026525mm Novo-Clean IC-Ag 25 70266

Novo-Clean Membranes13mm IC-H Membrane 10 7025425mm IC-H Membrane 10 7025547mm IC-H Membrane 5 7025625mm IC-Ag Membrane 10 70257

Accessories13mm Stainless Steel Filter Holder Ea 203825mm Stainless Steel Filter Holder Ea 2042

Two Chemistries Solve a Variety of Sample Preparation Problems

1. R. Saari-Nordhaus, L. M. Nair, J. M. Anderson, Jr., J. Chromatogr. 671 (1994) 159-163.2. R. Saari-Nordhaus and J. M. Anderson, Jr., J. Chromatogr. A. 706 1995) 563-569.


Sample Concentrator and Neutralizer

New! Alltech SCAN™ 1000 SampleProcessor

• Automates Sample Concentration andNeutralization for Improved Productivity

• Eliminates Costly Disposable SPE Devices withElectrochemical Neutralization

• Preconcentrates Large-Volume Trace-LevelSamples without Secondary Pumps

• Compatible with Any IC System

Alltech’s new dual-purpose SCAN™ 1000 Sample Processor*(Sample Concentration And Neutralization) automaticallyconcentrates and/or neutralizes samples much more effectivelyand conveniently than current techniques.

SCAN 1000 Sample Processor concentrates ultra-trace levelsamples for ppb and ppt anion and cation detection. No secondarypumps are required, as with coventional preconcentrationtechnique, because SCAN 1000 uses the IC system’s detectoreffluent to deliver the sample to the concentrator column. SCAN1000 Sample Processor is the only concentration techniquethat’s easily automated for multi-sample, unattended operation.

In the neutralization mode, SCAN 1000 Sample Processor treatsacidic or basic samples on-line as they are analyzed, eliminatinglabor-intensive manual off-line pretreatment techiques. Samplesare automatically delivered to the IC system free of interferences.Because SCAN 1000 regenerates a reuseable neutralization cellelectrochemically (patent pending) after each injection, costly,disposable SPE (solid-phase extraction) pretreatment cartridgesare eliminated. SCAN 1000 is easily automated to run continuouslywith any ion chromatography system.

*Patent Pending.

8640

Trace Cations in Concentrated HCl8641

The SCAN 1000 Neutralizes Acidic Sample Before CationAnalysis in Concentrated HCl

Untreated Treated

Column: Universal Cation, 100 x 4.6mmMobile Phase: 3mM Methanesulfonic AcidFlowrate: 1.0mL/minColumn Temp: 35°CDetector: Conductivity

0 4 8 12 16 Min. 0 3

1

2

3

4 5

6 9 12 15 18 Min.

1. Sodium (0.5ppm)2. Ammonium (0.3ppm)3. Potassium (0.2ppm)4. Magnesium (0.4ppm)5. Calcium (0.8ppm)

Instrum

ents

Analyze ppt Level Anions Easily with the SCAN 1000

Trace Anionsin Ultrapure Water

1. Chloride (500ppt)2. Sulfate (500ppt)

A Major Improvement Over ConventionalTechniques

SCAN 1000’s patent-pending system is a major improvementover conventional preconcentration and neutralization techniques.Consider these exclusive SCAN 1000 advantages:

- Conventional preconcentration techniques require a second pumpto deliver the sample to the preconcentration column. With SCAN1000, polished, deionized detector effluent does the job. No extrapumps are required.

- Conventional sample neutralization techniques are manual, laborintensive procedures that use costly, disposable SPE cartridges ortubes. SCAN 1000 Sample Processor is automated and uses anelectrochemically-regenerated neutralization cell that rarely requiresreplacement. Productivity increases and cost per analysis falls.

- Conventional preconcentration and neutralization techniques aredifficult or impossible to automate. SCAN 1000 is easily automated,offering continuous operation with any ion-chromatography system.

- Analytical data from SCAN 1000’s simple, computer-controlledoperation is significantly more precise and accurate than data fromconventional techniques.

3783


Flowrate: 1.2mL/minDetector: Suppressed ConductivitySample Volume: 4mL

0 4

1

2

8 12 16 Min.

8746

For SCAN 1000 application chromatograms,refer to pages 22-25.

See pages22-25


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Sample Concentrator and Neutralizer

Unique Valve Configuration Simplifies SampleConcentration and Neutralization

1111

00

99 88

7766

55

4433

22

SCAN 1000

From HPLC Pump

To Analytical Column

To ERIS or Waste

Detector Effluent

10Port

Valve From InjectionValve

To Injection Valve

ERIN Cell

Path during sample analysis

Path during sample neutralization

Polishing Column

Concentrator Column

SCAN™ 1000 Sample Processor uses an electrically-actuated10-port Rheodyne valve, a concentrator column, an ERIN™(Electrochemically Regenerated Ion Neutralization) cell, and aconstant current power supply. SCAN 1000 operates in twomodes: concentration and neutralization.

In either mode, sample is first loaded into an external sampleinjection valve either manually or with an autosampler. Next, theIC system’s detector effluent, which first flows through a polishingcolumn to remove ionic contaminants pushes the sample fromthe loop into SCAN 1000.

In the Neutralization mode, the sample flows through anERIN cell that’s packed with anion-exchange material in thehydroxide form or cation-exchange material in the hydrogenform. The hydrogen form cation-exchange ERIN cell is used foranalysis of anions in matricies that contain high concentrations

Microprocessor-Control for Simple User-Interface

SCAN 1000 Sample Processor has a simple user interface.Front panel controls are easy to operate and all importantparameters are displayed on a front-panel LCD window or easilyaccess with a single key-stroke. All steps are controlled by theSCAN 1000 software.

of hydroxide. The hydroxide-form anion-exchange ERIN cell isused for the analysis of cations in matricies that contain highconcentrations of hydronium ions. The neutralization reactionsthat take place in the ERIN cell are shown in Table 1.

Neutralized samples flow from the ERIN cell through aconcentrator column where the anionic or cationic analytes areretained by ion-exchange. Next, the SCAN 1000 valve rotatessending mobile phase through the concentrator column, elutingthe analytes onto an analytical column, where the separationoccurs. During the separation process, the ERIN cell iselectrochemically regenerated1 and equilibrated back to it’soriginal hyroxide or hydrogen form, ready to neutralize the nextsample. The process is repeated for each sample. Fullyautomated, unattended operation is possible when anautosampler is used.

In the Concentration mode, large volumes of trace-levelsamples are concentrated prior to analysis. The process isidentical to Neutralization, but the ERIN cell is replaced by aunion and electrochemical regeneration doesn’t take place. Inthis case, the polished detector effluent pushes the sample fromthe loop through SCAN 1000’s concentrator column where theanalytes are retained. Next, the SCAN 1000’s valve switches,sending mobile phase through the concentrator column, elutingthe analytes onto an analytical column, where the separationoccurs. SCAN 1000 lets you inject sample volumes (and masses)much larger than can be conveniently injected directly onto theanalytical column. With the 580 Autosampler, up to 10mLsample may be injected. This larger sample mass increases thedetector response, significantly improving sensitivity. Part-per-billion (ppb) and part-per-trillion (ppt) detection limits are routinelyacheived with the SCAN 1000.

1. R. Saari-Nordhaus and J. M. Anderson, Jr., Am. Lab., 1996; 28(3), 33N.

Acidic Sample:

R-(NH3)3+- OH- (cell) + H+- X- (acid sample matrix) --> R-(NH3)3+- X- + H2O where X = anions.

For acidic samples, the counteranions from the sample exchange with the hydroxide from the ERIN cell. The releasedhydroxide ions react with the hydrogen from the sample to form water. The sample cations are converted to their hydroxideforms and pass through the cell onto the concentrator column.

Basic Sample:

R-SO3- - H+(cell) + M+-(OH-) (basic sample matrix) --> R-SO3- - M+ + H2O where M+ = cations.

For basic samples, the countercations from the sample exchange with the hydrogen ions from the ERIN cell. The releasedhydrogen ions react with the hydroxide from the sample to form water. The sample anions are converted to their acid formsand pass through the cell onto the concentrator column.

ERIN Cells Neutralize Strongly Acidic or Basic Samples

Table 1


Sample Concentrator and NeutralizerI

nstruments


SCAN 1000 SAMPLE PROCESSOR

SCAN 1000*110V 635100220V 635101

*Must order ERIN cell, concentrator column, and indicator polishing cartridgesseparately.

SCAN 1000 SAMPLE PROCESSOR SPECIFICATIONS

Display: Backlit LCD; 4 line x 24 CharacterAlphanumeric

Line Voltage: 100-240 VAC, 50-60HZCell Voltage: ±25 VDCValve: Two Position 10-Port Electrically ActuatedFluid Path: All wetted parts are inert polymer:

PEEK (Polyetherether ketone)


CONCENTRATOR COLUMNS

Concentrator ColumnsAnion Concentrator Kit 635004

(contains: 1 holder and 3 anionconcentrator columns)

Anion Concentrator Column, 3/pkg 635005Cation Concentrator Kit 635006

(contains: 1 holder and 3 cationconcentrator columns)

Cation Concentrator Column, 3/pkg 635007Holder

Concentrator Column Holder (Guard 80101Cartridge Holder), Ea


POLISHING CARTRIDGES

Polishing CartridgesIndicator Polishing Cartridges, 25/pkg 635008High Capacity Polishing Cartridges Kit** 635011

(contains: 1 holder and 5 highcapacity polishing cartridges)

High Capacity Polishing 635012Cartridges, 5/pkg**

HoldersIndicator Polishing Cartridge 335001

Holder, 2/pkgHigh Capacity Polishing Cartridge Holder 635013

**High Capacity Polishing Cartridge is optional and not required to operate theSCAN 1000, especially if you are using a separate water pump as the samplecarrier. If you are using the detector effluent, this cartridge is stronglyrecommended.

The SCAN 1000 Simplifies Trace Cationic Analysis


1. Sodium (2.2ppb)2. Potassium (4.0ppb)3. Magnesium (1.1ppb)4. Calcium (1.1ppb)

0 3

1

2

3

4

6 9 12 15 18 Min.

8628

Trace Cations in Lead Frame


MODEL 580 AUTOSAMPLER*

Model 580 MF Autosampler w/ Large Volume Tray24 (10mL) Vials 580600

Field Installable OptionStandard Volume and Capacity Tray 580201

84 (1.5mL) and 3 (10mL) vials*For more information, please refer to page 60.


AUTOSAMPLER VIALS, CAPS & SAMPLE LOOPS

12 x 32mm Polypropylene(PP) Limited Volume Vials750µL Snap Top PP Vials 100 98050

11mm Caps for 12 x 32mm VialsSnap Top Clear Cap

with TFE/Silicone Liner 100 98034with TFE/Rubber Liner 100 98038

Larger Volume Vials, Supplied with Caps & Liners5mL Teflon PFA Vials 10 970507mL Teflon PFA Vials 10 970707mL Polyethylene Vials 100 97052

Liner for Larger Volume Teflon Vials23mm PTFE-faced Liner for 5mL, 7mL 36 95363

Flex-Connect Sample Loops (PEEK)5mL Ea 332247mL Ea 3322510mL Ea 33226


ERIN NEUTRALIZER CELLS

ERIN Cells Starter Kit 635001(contains one each of thebase (1.2meq) and acid(0.6meq) neutralizer cells)

ERIN Base Neutralizer1.2 meq Capacity Cell, Ea 6350020.6 meq Capacity Cell, Ea 635009

ERIN Acid Neutralizer0.6 meq Capacity Cell, Ea 6350030.3 meq Capacity Cell, Ea 635010


Conductivity Detector

2265

Inorganic Anions and Oxyhalides

1. Fluoride (1.3ppm)2. Chlorite (1.0ppm)3. Bromate (1.0ppm)4. Chloride (2.5ppm)5. Nitrite (2.5ppm)6. Phosphate (3.3ppm)7. Bromide (2.5ppm)8. Chlorate (4.0ppm)9. Nitrate (2.5ppm)


Column: Alltech Anion HC, 150 x 4.6mmMobile Phase: 2.8mM NaHCO3/2.2mM Na2CO3 in

10% MethanolFlowrate: 1.4mL/minDetector: Suppressed Conductivity

0 5 10 15 20 Min.

109

87653

2

14


1. Lithium (0.2ppm)2. Sodium (1.5ppm)3. Ammonium (1.5ppm)4. Potassium (2.5ppm)5. Magnesium (2ppm)6. Calcium (2ppm)

Cations by SCIC

1

2

3

4

5

6

0 16 Min.4 8 12

Baseline noise and drift are observed with conductivitydetectors that use temperature compensation.

The 550 Conductivity Detector’s active temperaturecontrol improves baseline stability.

0 2 Hrs.

Other Conductivity Detectors

The Alltech Model 550

0 2 Hrs

Alltech Model 550 Conductivity Detector• Detects Anions, Cations, Organic Acids, Metals,

and Surfactants• Advanced Microprocessor-Based Circuit

Improves Detection Sensitivity• Temperature Controlled Cell for Stable, Drift-Free

Baselines• Compatible with Suppressor-based and Single-

Column IC Methods• Method Program Capability for Convenience and

Reliability

Turn your HPLC system into an ion chromatograph with theAlltech 550 Conductivity Detector for fast, simple and inexpensivequantification of anions, cations, metals, organic acids andsurfactants down to ppb levels. The new micro-processor basedelectric circuitry improves the detection sensitivity of analytes byreducing baseline noise and drift. The detector cell is housed ina temperature controlled housing that isolates it from externaltemperature fluctuations. A unique heat exchanger heats themobile phase and the sample before they reach the detector cell.Both of these features provide stable baselines, free of temperatureinduced drift that is common with other conductivity detectors.

Use the 550 Conductivity Detector with or without chemicalsuppressors. Two types of chemical suppressors* are availablefrom Alltech for improving the sensitivity of ion analysis if itrequires parts per billion levels of quantification. If single-columnIC methods are chosen, the 550 detector autozero can offsethigh conductivity mobile phase for sensitive detection of ions inparts per million levels. Other conductivity detectors that aredesigned to work with suppressor-based IC methods lack thisfeature, so can only be operated with suppressor-based IC.

*The Alltech ERIS™1000HP Autosuppressor and The Alltech Model 335Suppressor.

7870

63368115

8114

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Conductivity Detector

3692

Need more information on the550 Conductivity Detector?

Request Bulletin #335

550 Detector Front Panel

The Model 550 Conductivity Detector is convenient and reliable.Front panel controls are easy to operate and all importantparameters are displayed on the LCD window. One-touchautozero offsets mobile phase background conductance. Thedetector cell-compartment temperature (“Set” and “Actual”) andconductance (“Output” and “Total”) are continuously displayedfor quick, accurate troubleshooting. Store and program up to 10methods for fast and accurate system set-up. An RS-232 serialport provides digital communication and control via an externalcomputer. The 550 detector can also be used as a conductivitymeter for measuring the absolute conductivity of solutions.


550 CONDUCTIVITY DETECTOR

Model 550 Conductivity Detector120V 550100240V 550101

Each 550 Detector is factory calibrated with traceableconductivity solutions for accurate conductivity measurements.Active temperature control eliminates temperature-inducedanalyte and background signal level changes (typically 2%/°C)that affect less sophisticated conductivity detectors. Themicroprocessor based electrical circuit of the 550 Detectorensures stable detector response, run-to-run, day-to-day andlab-to-lab.

Linearity Curve

2146Concentration (ppm)

SulfateNitriteBromide

00

10

20

30

10 20 30 40 50 60

Rela

tive

Peak

Are

a550 CONDUCTIVITY DETECTOR SPECIFICATIONS

Detection: Alternating Current, SynchronousDetection, 10KHz Frequency

Range: 0.01-5000 µS in 12 StepsNoise: Less than 0.0004µS

(DI Water, 1mL/min)Drift: Less than 0.004µS/hr.

(DI Water, 1mL/min, 35°C)Display: LCD, 2 x 20 CharactersAutozero: Offsets up to 10,000 µSTime Constant: 0.1, 0.5, 1, 5, 10 (sec), Software

SelectableMethod Storage: 1-10, User Defined Cell

CompartmentDimensions: 2.25" H x 2.25" W x 6.25" D

(5.72cm H x 5.72cm W x15.88cm D)Insulation: Minimum 1/2" All SidesHeater: 25W DC Heating Element

Vulcanized to Aluminum BlockPreheater: PEEK Tubing Knitted and Encased

in High Thermal Mass HousingCell Temp.: Factory set at 35°C, User Adjustable

Ambient to 60°CTemp. Precision: ±0.05°CCell Type: 2 Gold-plated SS ElectrodesCell Volume: 0.5µLPressure Rating: 400psigFluid Path: Wetted Parts Are Inert Polymers,

PEEK and Tefzel Electronic/Electrical

Output (analog): 10mV, 100mV, 1V Full Scale, SwitchSelectable

Output (digital): RS-232Computer Interface: RS-232 Serial CommunicationPower: 120/240V 50/60Hz, 100Watts

(Field Selectable)Dimensions: 7" H x 10" W x 12" D

(17.8cm H x 25.4cm W x 30.5cm D)Weight: 13lbs (5.9kgs)

Instrum

ents


Autosuppressor

2230

Solid-Phase Performance withContinuous Operation

The ERIS valve configuration allows endless cyclingbetween two suppressor cells for continuous instrumentoperation without interruption or suppressorreplacement.

Alltech ERIS™ 1000HP Autosuppressor• Electrochemical Self-Regeneration Delivers

Continuous, Reagent-Free Operation• Reliable Solid-Phase Chemistry Avoids

Membrane Suppressor Failures• Simple, Trouble-Free Operation Improves

Productivity• Meets US EPA method 300.0 Requirements• Compatible with Any IC or HPLC System

Alltech’s ERIS™ (Electrochemically Regenerated Ion Suppressor)1000HP Autosuppressor is the most versatile, reliable, productivesuppressor ever developed! An electrochemical technique (PatentPending) self-regenerates solid-phase suppressor cells. Thestable, reliable solid-phase replaces fragile, restrictive, trouble-prone membranes, while electrolysis of the detector effluent self-regenerates the solid-phase without regeneration systems orreagents. With the ERIS 1000HP, suppressor operation issimple, reliable, and economical.

A Revolutionary New Technique Eliminates FragileMembranes, Regeneration Systems, and Reagents

The Alltech ERIS 1000HP Autosuppressor consists of a 10-portvalve, two solid-phase electrochemical suppressor cells packedwith either cation (for anion analysis) or anion (for cation analysis)exchange resins, and a constant current power supply. Themobile phase from the analytical column flows through one cellat a time. While one cell suppresses the mobile phase, the otheris electrochemically regenerated and equilibrated. The valveswitches between cells at each injection, providing a freshsuppressor for each analysis.

Checking Suppressor Operation is Fast andConvenient

Eliminate the guesswork associated with checking ‘black-box’membrane suppressor operation. With the ERIS Autosuppressor,checking suppressor operation is quick and simple. The solid-phase resin used in the ERIS Cell is coated with an inert dye. Foranion analysis, the color changes from gold to magenta as thehydrogen is replaced by mobile phase and sample counter-cations. For cation analysis, the color changes from blue to beigeas the hydroxide ion is replaced by mobile phase and samplecounter-anions. The solid-phase suppressor bed is alwaysvisible, and an inert dye continuously indicates suppressorstatus.

2202

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3693

Need more information on the ERIS1000HP Autosuppressor?

Request Bulletin #334A or CallAlltech for a Demo or a Quote.


Autosuppressor

Microprocessor-Control for Simple User-Interface

The Alltech ERIS™ 1000HP Autosuppressor has a simple, intuitiveuser interface for reliable operation by any skilled lab personnel,from technician to PhD level! Just turn on the power, enter thecell type (anion or cation), mobile phase concentration, flowrate,and run time. The Alltech ERIS 1000HP Autosuppressor controlsthe valve switching, regeneration, and equilibration. No separatemodules, reagents, or external control from other instruments isrequired! The Alltech ERIS 1000HP Autosuppressor is compatiblewith all IC and HPLC systems.

Regeneration Screen - Display critical electrochemicalregeneration parameters at any time with a single key-stroke.

Analysis Screen - All user entries are prompted for quick,intuitive method set-up.

ERIS Front Panel - Eight clearly labelled keys and abright, back-lit LCD control and display all ERISfunctions.

Improve Sensitivity, Baseline Stability, andResolving Power

The ERIS™ Autosuppressor improves IC detection sensitivity,baseline stability, and resolving power. ERIS simultaneouslyreduces the mobile phase’s conductivity and increases analyteconductivity, improving detection sensitivity by up to two ordersof magnitude. Since the mobile phase’s conductivity is reduced,the baseline stability is also improved and gradient elution ispossible. Monitoring inorganic anions in water by US EPAguidelines requires low detection limits, high column efficiency,and reproducible retention times and peak area. The ERIS1000HP meets these rigorous requirements.

Monitor Inorganic Anions inWater per US EPA Method 300.0

Column: Allsep Anion, 100 x 4.6 mmMobile Phase: 0.85mM NaHCO3/0.9mM Na2CO3


8542

Analyze ppb Level Cations Easilywithout Preconcentration

Column: Universal Cation, 100 x 4.6mmMobile Phase: 3mM Methane Sulfonic AcidFlowrate: 1.0mL/minDetector: Suppressed Conductivity

1. Lithium (10ppb)2. Sodium (150ppb)3. Ammonium (70ppb)4. Potassium (175ppb)5. Magnesium (50ppb)6. Calcium (50ppb)

0 5 10 15 20 Min.

1

2

3 4

56

8089

“The ERIS unit was easy to install and has workedextremely well for our low level ammonium analysis.

Coupled with our Alltech Autosampler, we’ve been able toachieve great results in a high throughput environment.”

Craig Just, University of Iowa

12

3

4 56

7

0 2 4 6 8 10 Min.

1. Fluoride (20ppb)2. Chloride (40 ppb)3. Nitrite-N (12ppb)4. Bromide (40 ppb)5. Nitrate-N (9ppb)6. Phosphate-P (19ppb)7. Sulfate (60ppb)

Instrum

ents


ALLTECH ERIS 1000HP AUTOSUPPRESSOR

Display: Backlit LCD; 4 line x 24 CharacterAlphanumeric

Line Voltage: 100-240 VAC, 50-60HzCell Voltage: ±25 VDCValve: Two Position 10-Port Electrically ActuatedRemote out: Contact Closure (NO or NC) on ErrorInject: External Injection Contact ClosureRemote In: Trigger Standby Mode on External ErrorDimensions: 4.1" H x 10" W x 18.1" D

(10.5cm H x 25.4cm W x 46cm D)Weight: 15lbs (6.8 kg)Fluid Path: All wetted parts are inert polymers:

PEEK (Polyetherether ketone)

Anions and Oxyhalides perU.S EPA Method 300.0

Unlike some self-regenerated membrane suppressors, theAlltech ERIS 1000HP Autosuppressor operates reliablywith organic containing mobile phases. There is no needto change operating mode or supply external reagent withorganic containing mobile phases.

Column: Anion HC, 150 x 4.6mmMobile Phase: 2.8mM NaHCO3/2.2mM Na2CO3 in 10% MeOHFlowrate: 1.4mL/minDetector: Suppressed Conductivity

1. Fluoride (3ppm)2. Chlorite (6ppm)3. Bromate (6ppm)4. Chloride (6ppm)5. Nitrite (6ppm)6. Phosphate (6ppm)7. Bromide (6ppm)8. Chlorate (6ppm)9. Nitrate (6ppm)

10. Sulfate (6ppm)

0 5 10 15 20 25 Min.

10

987

65

3

2

1 4

Reliable Solid-Phase Chemistry SimplifiesOperation and Reduces Maintenance

The Alltech ERIS™ 1000HP Autosuppressor’s rugged solid-phase cells tolerate high backpressure, and electroactive and/ororganic-containing mobile phases. Cells are made from inert,durable components for extended lifetime. Unlike membrane-based suppressors, ERIS Cells tolerate high backpressure andwon’t rupture or deform if an upstream blockage occurs. Thereare no tedious backpressure adjustments, and no routinemaintenance, minimizing operating costs and downtime.

Advanced Chemistry Eliminates the Drawbacks ofEarly Packed-bed Suppressors

Early packed-bed suppressors, introduced in 1975, were nomatch for the analytical performance of today’s membranesuppressors. They required periodic regeneration and sufferedfrom band broadening, Donnan exclusion, and oxidation of nitriteto nitrate. ERIS 1000HP’s unique small-volume cells and advancedsolid-phase chemistry eliminate these drawbacks, providingexcellent analytical performance along with rugged durability,reliability, and versatility membrane suppressors can’t match.

Extending Suppression Capabilities to MoreMethods and Instruments

The Alltech ERIS 1000HP Autosuppressor is the most flexible,versatile suppressor system available. It’s the only self-regenerating suppressor versatile enough to work with organiccontaining and electroactive mobile phases, providing moreoptions for suppressor-based method. Unlike membrane-basedsuppressors, sold for exclusive use on one manufacturer’ssystem, when you buy Alltech ERIS 1000, you choose where,when, and how to use it. Purchase the Alltech ERIS 1000HPAutosuppressor alone to upgrade existing IC or LC systems,regardless of manufacturer, or as part of a complete Alltech ICsystem. The choice is yours.

8009

3147

AutosuppressorI

nstr

umen

ts


Use the ERIS 1000HP Autosuppressor for On-LineSample Preparation

The ERIS 1000HP Autosuppressor may be used as anelectrochemically regenerated guard column to protect yourexpensive organic acid columns. Samples containing metal ionscontaminate organic acid column packing, causing a gradualloss in capacity and eventually, column failure. The ERIS1000HP Autosuppressor overcomes these problems by removingsample contaminants on-line. Simply install the ERIS 1000HPbetween your injector and the Organic acid column.

Description Part. No. Price

ALLTECH ERIS 1000 AUTOSUPPRESSOR

Alltech ERIS 1000HP Autosuppressor*120 VAC (Cells not included) 535101240 VAC (Cells not included) 535102

*Must order cells separately.

526 HPLC Pump

320 Conductivity Detector

ERIS™ 1000HP Autosuppressor

When sample passes through the ERIS cell, it removesthe components that would otherwise retain and damagethe analytical column.

“We are using anion-exclusion columns for ourorganic acid analysis. The performance and lifetime

of these columns is greatly affected by cation contaminationpresent in the sample. We decided to use ERIS as aguard column to remove the cations before reaching theanalytical column. We installed ERIS between the injectorand the analytical column. We found that the Alltech ERISAutosuppressor works very well for this application. Theperformance of our anion-exclusion column is improvedand the life expectancy of the column increasedsignificantly.”

Jim Steinke, Cargill, Inc.

Autosuppressor

For more information on using the ERIS 1000HPAutosuppressor to protect your organic acid columns,

please request Application Note #0035.

HPLCPump

Injector

ERIS™ 1000HPAutosuppressor

Detector

AnalyticalColumn

Data System

Instrum

ents

Instrument Configuration for On-LineSample Preparation

Read What ERIS Users Have to Say:

Description Part. No. Price

AUTOSUPPRESSOR CELLS†

Standard Suppressor Cells (1500psig pressure limit)Suppressor Cell Kit

Contains two each of anion and 535001cation suppressor cells (14 x 7.5)

Anion Suppressor Cells14 x 7.5mm, 2/pkg 5350020Low Capacity, 7 x 7.5mm, 2/pkg 5350030

Cation Suppressor Cells14 x 7.5mm, 2/pkg 5350040

High Pressure (HP) Cells (3000psig pressure limit)HP Suppressor Cell Kit

Contains two each of the HP Anion 535010and cation suppressor cells (14 x 7.5)

HP Anion Suppressor CellsNormal Capacity, 14 x 7.5mm, 2/pkg 535011Low Capacity, 7 x 7.5mm, 2/pkg 535012

HP Cation Suppressor CellsNormal Capacity, 14 x 7.5mm, 2/pkg 535014Low Capacity, 7 x 7.5mm, 2/pkg 535015

High Pressure (HP) PEEK Cells (5000psig pressure limit)HP PEEK Anion Suppressor Cells

Normal Capacity, 14 x 7.5mm, 2/pkg 535021Low Capacity, 7 x 7.5mm, 2/pkg 535022High Capacity, 21 x 7.5mm, 2/pkg 535023

HP PEEK Cation Suppressor CellsNormal Capacity, 14 x 7.5mm, 2/pkg 535024Low Capacity, 7 x 7.5mm, 2/pkg 535025High Capacity, 21 x 7.5mm, 2/pkg 535026

Narrowbore High Pressure (HP) PEEK Cells(5000psig pressure limit)

Narrowbore HP PEEK Anion Suppressor CellsNormal Capacity, 15 x 4.6mm, 2/pkg 535031Low Capacity, 8 x 4.6mm, 2/pkg 535032

Narrowbore HP PEEK Cation Suppressor CellsNormal Capacity, 15 x 4.6mm, 2/pkg 535034Low Capacity, 8 x 4.6mm, 2/pkg 535035

† Two types of cells are available. The standard cell (Part No. 535001,5350020, 5350030, and 5350040) can tolerate up to 1500psig pressure. Thiscell can beused with the ERIS 1000 and ERIS 1000HP Autosuppressors. Thenew High Pressure (HP) cell (Part No. 535010, 535011, 53512, 535014,535015) can tolerate up to 3000psig pressure. This cell can be used only withthe ERIS 1000HP Autosuppressor. Your applications will determine which cellto use. The standard 1500psig pressure limit cell is more than sufficient for ICanalysis. The HP cell is recommended when your applicationsrequire morethan 1500psig pressure, such as interfacing ERISwith your mass spectrometerto remove buffers from the mobile phase, or using ERIS as a samplepreparation device. If your applications require more than 3000psigpressure,cells that are made of PEEK (polyetherether ketone) housing arerecommended. These cells withstand up to 5000psig pressure. The PEEKhousing cells are not transparent and you are not able to see the color changeas the suppressor is being used. Narrowbore cells are also available in PEEKhousing. These cells are recommended for use with 2.1mm or smaller IDcolumn. The pressure limit on narrowbore cells is 5000psig.


Need more information on the 335 Suppressor Module?

Request ISS #019 orCall Alltech for a Demo or a Quote.

Suppressor

Low Level Anions and Oxyhalidesper US EPA Method 300.0, Part B

1. Fluoride (0.5ppm)2. Chlorite (0.5ppm)3. Bromate (0.5ppm)4. Chloride (1.0ppm)5. Nitrite (1.0ppm)6. Phosphate (1.5ppm)7. Bromide (1.0ppm)8. Chlorate (5.0ppm)9. Nitrate (1.0ppm)


Column: Anion HC, 150 x 4.6mmMobile Phase: 2.8mM NaHCO3/2.2mM Na2CO3 in

10% MethanolFlowrate: 1.4mL/minDetector: Suppressed Conductivity

0 5 10 15 20 Min.

109

8

7652

3

1

4

Household Cleanser

Column: Surfactant/R, 150 x 4.6mmMobile Phase: A: 2mM Nonafluoropentanoic Acid

B: AcetonitrileGradient: Time: 0 5 8 20

% B: 30 30 35 80Flowrate: 1.0mL/minDetector: Suppressed Conductivity

SPCS Eliminates Regeneration Systems, Reagents,and Maintenance

SPCS uses a unique valve configuration with two disposablesuppressor cartridges. While one cartridge provides suppression,the other is equilibrated or replaced. Depending on your mobilephase ionic strength and flowrate, each SPCS cartridge lastsbetween 7-12 hours for anion analysis, and between 4-6 hoursfor cation analysis. Changing the disposable SPCS cartridge isfast and easy, and since there is no periodic regeneration, yoursystem runs uninterrupted as long as personnel are available tochange cartridges. For fully unattended operation, use theAlltech ERIS™ 1000HP Autosuppressor (see page 50-53).

The rugged suppressor cartridges are inexpensive,disposable, and require no maintenance.


MODEL 335 SUPPRESSOR MODULE

Model 335 Suppressor Module 335100(Includes Two Holders and25 Anion cartridges)

Anion SPCS Cartridges, 25/pkg 335002Cation SPCS Cartridges, 25/pkg 335003

Alltech Model 335 Suppressor Module• Meets US EPA Method 300.0 Requirements• Convenient Disposable Cartridges• Installs Easily and Quickly in Any IC or HPLC

System

Chemical suppression offers many advantages for ion analysis,including stable baselines, ppb level anion and cation detectionsensitivity, and gradient capabilities. Alltech’s Solid-PhaseChemical Suppressor (SPCS) lets you enjoy all the advantagesof chemical suppression for less than the cost of some columns!Add SPCS to any IC or LC system that has a conductivitydetector. Explore new separations or run existing suppressor-based methods, including EPA method 300.0 part A and B.

3602

8133 8650

0

1

2

34

4 8 12 16 20 Min.

1. Dimethyl ammonium2. Dioctyl ammonium3. Octyl decyldimethyl

ammonium4. Didecyl dimethyl

ammonium

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Column Heater

ALLTECH 530 COLUMN HEATER

Temp. Control: Ambient to 85°CTemp. Stability: ±0.05°CHeated Zone Dimensions: 1.3" H x 3.5" W x 17.6" D

(3.30cm H x 8.89cm W x 44.70cm D)Power: 110V, 220V

The Alltech Model 530 Column Heater• Unique Drawer Provides Fast, Convenient Access

to Columns and Conserves Bench Space• Microprocessor Controlled for Improved

Temperature Precision (±0.05°C)• Continuous Monitoring of “Set” and “Actual”

Temperature

Improve the baseline stability, retention time reproducibility, andpeak area precision of your IC and HPLC analysis with the AlltechModel 530 Column Heater. The Alltech Model 530 ColumnHeater provides precise temperature with an accuracy of 1°Cand control stability of ±0.05°C. The temperature can be setbetween ambient and 85°C using the white arrows on the frontpanel. The column chamber has a built-in preheater consisting oftwo feet of 0.01" PEEK tubing cast in tin alloy block, stainlesssteel preheater assembly is also available. This preheater heatsthe mobile phase and sample before they reach the column. Thetemperature within the column chamber is maintained within±0.05°C of the set point by a sophisticated microprocessor-based sensor/control circuit. The LED display in the front panelshows the “Set” and “Actual” temperature.

The Alltech Model 530 Column Heater is designed with aunique drawer for quick, easy access to column chamber withoutremoving the heater cover. All fluid connections are made fromthe front panel. The column drawer is pulled out to install thecolumns. A large column chamber (1.3" high, 3.5" wide, and17.6" deep) accepts two columns plus guard columns fromanalytical to semi-prep size (1/2" OD x 300mm long). A prepcolumn up to 1" OD x 300mm long can also be used with the 530heater. The column chamber is well insulated to provide maximumtemperature stability. The column chamber is also equippedwith a drain line to direct liquid from leaks safely away fromelectronics to a bulkhead union on the instrument back panel.

Because the 530 Column Heater is easily accessed withoutremoving the instrument cover, it may be stacked with otherequipment to reduce bench space requirements. A mountingbracket is provided to install an injection valve or switching valveon the side panel.


MODEL 530 COLUMN HEATER

Model 530 Column Heater110V 530100220V 530101

AccessoriesPEEK Preheater Assembly 330110Stainless Steel Preheater Assembly 330111

3600

Column preheater ensures enhanced thermal stability

3601

Unique drawer provides fast, convenient access tocolumns and conserves bench space

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HPLC Pumps


MODEL 526 HPLC PUMP

Isocratic Pump SystemStandard, Metal-Free

110V/50-60 Hz 526100220V/50-60Hz 526101

Standard, Stainless Steel110V/50-60Hz 526200220V/50-60Hz 526201

Macro, Metal-Free110V/50-60Hz 526300220V/50-60Hz 526301

Macro, Stainless Steel110V/50-60Hz 526400220V/50-60Hz 526401

Binary Gradient Pump System(Includes 2 pumps, an injector mounting bracket, a mixingtee, a mixing coil, tubing and fittings)Standard, Metal-Free

110V/50-60Hz 526105220V/50-60Hz 526106


Macro, Metal Free110V/50-60Hz 526315220V/50-60Hz 526316


AccessoryHigh Pressure Gradient Kit 105526

(Includes tubing, fittings, and acable to set up a gradient system)

Alltech Model 526 HPLC Pump• Ideal for Sensitive Research and Method

Development Applications• Multiple Event and Input/Output Triggers for

Remote Operation• Built-in Pulse Dampener and Digital Piston Refill

for Pulse-Free Operation

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MODEL 526 HPLC PUMP SPECIFICATIONS

Max. Operating Pressure: 0 to 6000psig (Standard)0 to 1500psig (Macro)

Flowrate Range: 0.01 to 10mL/min (Standard)0.04 to 40mL/min (Macro)

Flowrate Precision: 0.2% RSDFlow Accuracy: 2.0%

3603

526 426 301Function Pump Pump Pump

526, 426, AND 301 PUMP COMPARISON

Built-in Pulse Dampener: Yes Yes YesSelf-Flush Pump Head: Yes Yes YesSetable Upper and Lower

Pressure Limit: Yes Yes NoSystem Pressure Display: Yes Yes NoExternal Voltage/Frequency

Interface: Yes Yes NoExternal Event Terminal: Yes No NoUpgradeable for High Pressure

Binary Gradient: Yes No NoUpgradeable with 527 Gradient

Controller for Up to 4-SolventLow Pressure Gradient: Yes Yes No

The Model 526 Pump is a high performance pump idealfor analytical method development and research applications.This pump features a single piston with digitally-controlled refillstroke and a patented low dead-volume pulse dampener forvirtually pulse-free operation. This pulseless technologyimproves pulse-sensitive electrochemical, conductivity andrefractive index detector performance. High ionic strengthmobile phases, which cause excessive seal wear in other pumps,are not a problem with the 526 Pump. The automatic seal washfeature extends the pump seal life by flushing the back of theprimary seal with a flushing solution. This feature and a sealedmembrane keypad assure years of trouble free operation. Thispump carries a three year warranty.

Use a single 526 Pump for demanding isocratic applications.Add a second 526 Pump to form a high pressure binary gradientsystem. The second pump operates as a slave to the first pump,and all required software is built in. Add the 527 GradientController (see page 58) to perform low pressure binary, ternary,or quaternary gradient. External devices such as autosamplersand switching valves can be triggered from the 526 Pumpsoftware. An RS-232 port and relay outputs are standard, andpermit the 526 Pump to control or be controlled by other devices.

The 526 Pump is available in either 316 stainless steel ormetal-free PEEK (polyetherether ketone) for metal sensitiveapplications. The standard pump is programmable from 0.01to 10mL/min, making it suitable for both micro and standardLC applications. A macro pump is available for preparativeapplications and is programmable from 0.04 to 40.0mL/min.


PUMP HEAD KITS FOR MODELS 526, 426, 301 PUMPS

Metal-Free Pump Head KitsStandard 120800Macro 120802

Stainless Steel Pump Head KitsStandard 120799Macro 120801


HPLC Pumps

3788

MODEL 426 & MODEL 301 LC PUMP SPECIFICATIONS

Max. Operating Pressure: 0 to 6000psig (Standard)0 to 1500psig (Macro)

Flowrate Range: 0.01 to 10mL/min (Standard)0.04 to 40mL/min (Macro)

Flowrate Precision: 0.2% RSDFlow Accuracy: 2.0%

Alltech Model 426 and 301 HPLC Pumps• Built-in Pulse Dampener and Rapid Refill for

Pulse-Free Operations• Self-Flushing Pump Head for Longer Seal Life• Automatic Prime/Purge System Simplify Priming• LED Display and Keypad for Monitoring Pressure

and Changing Flowrates

Model 426 HPLC Pump

The Alltech Model 426 HPLC pump is a digital, isocratic pump foradvanced applications. This pump features a single piston,patented low dead volume pulse dampener and rapid refill toprovide virtually pulse free operations. The flowrate can be setfrom 0.01 to 10mL/min. A macro version is available for preparativeapplications to set the flowrate from 0.04 to 40 mL/min. The pumpseal life is extended by flushing the back of the primary seal witha flushing solution. The self flush is automatic and the seals canbe changed in five minutes without any tools. An automaticprime/purge button makes it easy to prime or purge the pump.The upper and lower pressure limits can be set easily and thepressure can be monitored on the LED display.

The Model 426 Pump also provides several interfacing featuresfor convenient pump control from other instruments such asautosamplers, integrators, and computers. An RS-232C modularjack can be used for remote pump operation via a PC and theappropriate software. Other features include PUMP-RUN andPUMP-STOP for basic pump control.

The Model 426 Pump is a low cost high performance pumpideal for any isocratic, semi-preparative to preparative, researchand development, quality assurance and educational applications.Add the 527 Gradient Controller to upgrade the 426 to a gradientpump.

Model 301 HPLC Pump

The Alltech 301 HPLC Pump is an isocratic, single piston pumpfor basic applications. This pump has a built-in low dead volumepulse dampener and rapid refill for pulse-free operations. Theflowrates can be set from 0.01 to 10 mL/min. An optional macropump is available for preparative applications allowing theflowrates from 0.04 to 40 mL/min. The Model 301 pump requiresvery low maintenance due to its self-flushing pump head. Anautomatic prime/purge button makes it easier to prime the pump.This low price pump is ideal for any isocratic applications.Available in both stainless steel and PEEK formats for yourspecific applications.

NOTE: The Model 301 HPLC pump does not monitor pressureand you cannot set the upper and lower pressure limits.


MODELS 426 AND 301 HPLC PUMPS

Model 426 HPLC PumpStandard, Metal Free

110V/50-60 Hz 426100220V/50-60Hz 426101




Model 301 HPLC PumpStandard, Metal Free

110V/50-60 Hz 301100220V/50-60Hz 301101




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See page56

1529A

Model 426 HPLC Pump

Model 301 HPLC Pump


Gradient Controller and Degassing System

On-Line Degassing System• Improves LC Precision

and Accuracy• Eliminates Regassing

with NO-OX™ Tubing

The Alltech On-Line DegassingSystem continuously removesdissolved gases from the mobilephase as it is delivered to your LCsystem. By eliminating dissolvedgases, you eliminate flow stabilitiesand noisy drifting baselines.

The degasser is economical and easy to use. Simply attachyour solvent reservoir(s) and pump inlet lines to the Alltech On-Line Degassing System and turn on the power. Degassingbegins immediately. The system comes complete with fittingsand tubing to connect up to four solvents.

The Alltech On-Line Degassing System is the most effectivevacuum degassing system available. Its superior function comesfrom a unique tubular membrane (U.S. Patent No. 5,340,384)which carriers your solvent through a vacuum chamber to yourpump. The vacuum draws the dissolved gases out through themembrane wall. Another key feature is the two-layer design ofthe NO-OX Tubing. It combines a chemical inert inner layer toprevent regassing as the degassed solvent leaves the vacuumchamber.

ON-LINE VACUUM DEGASSING SPECIFICATIONS

Number of Channels: 4Degassing Efficiency: 0.5ppm at 1mL/min

Starting with Water Saturated withO2 at 8.1ppm at 25°C

Wetted Parts: PEEK, TeflonDead Volume/Channel: 15mLMax. Flowrate/Channel: 10mL/minMax. Pressure/Channel: 5psigVacuum Level: 27" of HgDimensions: 3.5" W x 6.87" H x 14.37" D

(8.9cm" W x 17.5cm H x 36.5cm D)Weight: 7.5lbs (3.4kg)Power: 100/120/220/240VAC,

50/60Hz Selectable


ON-LINE VACUUM DEGASSING SYSTEM

On-Line Vacuum Degassing System100/120/220/240VAC, 50/60Hz 590100

(Includes: 12ft NO-OX Tubingand 8 NO-OX Fittings

Replacement PartsDegassing Channels Using Modular 590114

Channels for Part No. 590100 OnlyRebuild Damaged Degassing Channels 590112Vacuum Sensor 590113

The New 527 Gradient Controller• Modular - Upgrades

Alltech Model 426 and526 Pumps to GradientPumping Systems

• Versatile - Capable of LowPressure or HighPressure Mixing

The Model 527 Gradient Controllerprovides accurate, precise solventcomposition and flow control for ICor HPLC systems. The 527controller is compatible with Alltech 426 and 526, stainless steelor metal-free pumps. Simple isocratic systems can easily beupgraded to high performance gradient systems.

The versatile 527 Gradient Controller is capable of low pressureor high pressure gradient mixing. One pump with the 527 easilybecomes a low pressure quaternary gradient system whichcontrols solvent composition by proportioning valves. Thisconfiguration is more economical and works well for almost allapplications. For enhanced gradient control up to four pumpswith the 527 can be combined for a high pressure quaternarysystem. The 527 controls the flowrate of each pump in highpressure mode which is ideal for small changes in solventcomposition over long periods of time.

The 527 Gradient Controller stores up to ten methods. It linksmethods and interfaces to autosamplers for true multi-methodautomation. External events can be programmed into themethods to extend the system control and automation capability.These events can be used to trigger valves, or sample collectors.

A high quality vacuum degasser is recommended for improvinggradient performance.


ALLTECH MODEL 527 GRADIENT CONTROLLER

Model 527 Gradient ControllerFor 526 HPLC Pump 527100For 426 HPLC Pump 527200

527 GRADIENT CONTROLLER SPECIFICATIONS

Gradient Mixing Modes: Low or High-PressureGradient Percent Range: 0-100% in 0.1% IncrementsGradient Profiles: Concave, Convex, or Linear.

Ten Curve ChoicesProgramming: 10 Methods with Up to 50

Event Lines per MethodAnalysis Time: 999.9 min.Flowrate Range: 0.01-9.99mL in 0.01 StepsDisplay: 4 Lines x 20 Characters LCDOutputs: Four Contact Closure RelaysInputs: Remote Start and Stop,

TTL or Contact ClosureDimensions: 10.4" W x 17.5" D x 5.5" H

(26.4cm W x 44.6cm D x 14cm H)Weight: 24lbs (11kg)Power: 100/120/220/240VAC, 50/60Hz

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09243786


LINEAR MODEL 200 SPECIFICATIONS

Optical Design: Concave Holographic GratingMonochromator with Dual-BeamOptics

Wavelength Range: 190-800nm with Deuterium andTungsten Lamps

Wavelength Accuracy: ±1.0nmWavelength Precision: ±1.0nmSpectral Bandwidth: 6nmNoise: <±1.0 x 10-5 AU @ 254nmDrift: <2 x 10-4 AU/hour After Warm-UpLinearity: <1% to 2.0 AUAbsorbance Range: 0.0005-2.0 AUFSAutozero Range: >1.5 AUDigital Display: 3-Digit LEDIntegrator Output: 1 AU/VRecorder Outputs: 10mV, 100mV, 1V FSPower: 100/120/220/240V; 50/60HzDimensions: 6.3" H x 13.4" W x 9.8" D

(16cm H x 34cm W x 25cm D)Weight: 20lbs (9.1kgs)

Linear Model 200 Variable WavelengthUV/Vis Detector

• Simple Analog Controls• Interchangeable Flowcells and Lamps

The advanced optical design in the Model 200 provides anoperating range of 190 to 800nm. Dual-beam optics reduce noiseand drift to minimum levels, allowing effective operation at 0.0005AUFS. The low level of stray light allows a linear range of 1% to2 AU. Refractive index effects have been greatly reduced,permitting high sensitivity isocratic and gradient applications.

The Linear 200 is designed with a unique optical system. Ahigh-efficiency, concave holographic grating provides operationin the full wavelength range, reducing the need for other attenuatingoptics and lenses. Combined with a special quartz fiber-opticbeam splitter, this optical design results in high transmittance andlow noise. Stray light is minimized for high linearity andextraordinary accuracy.

The Model 200 comes ready to use with a deuterium lamp formeasurements for 190 to 365nm and either a stainless steel orKel-F 6mm, 9µL analytical flow cell. A tungsten lamp is availablefor detection in the visible range from 366 to 800nm. Only onelamp can be mounted on the detector at a time, but all lamps areprealigned for easy installation. Choose among ten differentinterchangeable flowcells, from capillary to preparative.


LINEAR MODEL 200 UV/VIS DETECTOR

Model 200 Detector*With 6mm, 9µL SS Flowcell 27410000With 6mm, 9µL Kel-F Flowcell 27410010With No Flowcell 274100

LampsDeuterium Lamp and Assembly 2037077Tungsten Lamp and Mounting Plate 2037079

*Other UV detectors are available. Please contact your Alltech representative.

3604

Path Flowcell Tubing Maximum HeatLength Type Volume Max. Flow Material ID/OD Pressure Exchanger Part No. Price

INTERCHANGEABLE FLOWCELLS FOR LINEAR 200 AND 205/201 DETECTORS

6mm Analytical 9µL 50mL/min SS/TFE 0.01/0.063" 1000psig Yes 203707310mm Analytical 15µL 50mL/min SS/TFE 0.01/0.063" 1000psig Yes 20370876mm Bio-Analytical 9µL 50mL/min Kel-F 0.01/0.063" 500psig Yes 20370766mm Bio-Analytical 9µL 50mL/min Ti/Kalrez 0.01/0.063" 1000psig Yes 20370713mm Microbore 1.2µL 10mL/min SS/TFE 0.005/0.063" 1000psig Yes 20370746mm Microbore 5µL 20mL/min SS/TFE 0.007/0.063" 1000psig Yes 20370823mm Semi-Prep, 4.5µL 100mL/min SS/TFE 0.03/0.063 1000psig Yes 2037075

Open Column0-3mm Preparative 0-4.6µL 6000mL/min SS/Kalrez 0.087/0.125" 2000psig No 20370830-3mm Bio-Prep 0-4.6µL 6000mL/min Ti/Kalrez 0.087/0.125" 2000psig No 20370932mm High-Pressure 0.25µL 5mL/min SS/PEEK 0.005/0.063" 7000psig No 2037095

Microbore0.24mm Off-Column 0.35µL 0.5mL/min Fused Si N/A 5000psig No 2037094

CapillaryOn-Column – – – – – – 2037072

CapillaryCuvette Cell – – – – – – 2037081

Holder** (for 10mm ID, 12mm OD Cuvettes)

UV/VIS DetectorI

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VIALS

1.5mL 12 x 32mm Glass VialsStandard Mouth Crimp Vials 100 66002Wide Mouth Crimp Vials 100 98003Wide Mouth Snap Ring Vials 100 98030

12 x 32mm Polypropylene (PP) Limited Volume Vials750µL Snap Top PP Vials 100 98050

11mm Caps for 1.5mL VialsSeal with TFE/Silicone, 3/37mils 100 98740Silver Seal w/TFE/Butyl Rubber Liner 100 73070Snap Top Cap with 100 98034

TFE/Silicone LinerSnap Top Cap with 100 98038

TFE/Butyl Rubber LinerLarge Volume Glass Vials

5mL, 20 x 38mm Vials (for 570 only) 100 6613010mL, 23 x 46mm Vials (for 580 only) 100 6655

Large Volume Teflon PFA Vials, Supplied w/Caps & Liners5mL, 25 x 35mm Vials (for 580 only) 10 970507mL, 25 x 35mm Vials (for 580 only) 10 97070

Liner for Larger Volume Teflon Vials23mm PTFE-faced Liner for 5mL,7mL 36 95363

20mm Caps for 5mL VialsSeal with TFE/Silicone, 125mils 72 95587Silver Seal w/TFE/Butyl Rubber Liner 100 98801

Model 580 Autosampler• Multifunctional

AutosamplerImproves LaboratoryEfficiency

• Performs AccurateSample Dilutions

• Automates Pre-ColumnDerivatization

• Delivers InternalStandards to Sample Vials

• Provides Three Injection Techniques forMaximum Versatility

Improve laboratory efficiency by automating sample preparationtasks with the Alltech Model 580 Autosampler. Syringe controlguarantees superior precision for reagent addition during sampledilution, derivatization, and internal standard addition. The highresolution syringe mechanism breaks down each microliter ofvolume into 24 motor-controlled steps. This accurately aspiratesand dispenses small volumes. Therefore, solvent and dilutedsample waste are minimized decreasing disposal costs. Reagentaddition methods contain up to 15 program lines, includingmixing. Rapid dispensing thoroughly mixes samples and reagentsinto programmed vials.

A unique sequence of valve switching and syringe samplewithdrawal maximizes the injection volume range from a fewmicroliters to milliliters. The Model 580 Autosampler offers threeloop filling options. Flushed loop injections overfill the sampleloop by up to 3X resulting in better than 0.3% reproducibility.Partial loop injections provide programmable injection volumeswithout hardware changes for maximum flexibility. When samplewaste cannot be tolerated, µL pick-up injections are ideal.

The 580 Autosampler’s intelligent software performs selfdiagnostic tests. The display will alert you to problems with theinjection valve, syringe dispenser, injection needle, sample tray,or missing vials. An alarm output will be activated if an erroroccurs to shutdown the HPLC pump or to control other instruments.The software records valve rotor seal and syringe plunger sealmovements. Preventive seal maintenance can be executedbefore performance degrades. Up to nine methods can bestored and linked in series for the run sequence.

The Alltech Model 580 Autosampler comes with a standardsample tray which holds 84 sample vials (1.5mL) and 3 reagentvials (10mL). A large volume sample tray is optional which holds24 (10mL) vials for semi-prep to preparative injection volumes.A large capacity tray is also available which holds 96 (1.5mL)vials. A built in column heater is standard for the Model 580.Column temperature control combined with the optional samplecooling ensures consistent results. The Rheodyne series 9000metal-free valve option is ideal for ion sensitive or biologicalapplications.


ALLTECH MODEL 580 AUTOSAMPLER

Standard Volume and Capacity Autosamplerwith 84 (1.5mL) and 3 (10mL) Vial Tray 580100with Sample Cooling Option 580300with Metal-Free Valve 580500with Sample Cooling Option, Metal-Free 580700

Field Installable OptionsLarge Volume Tray, 580200

24 Vial Capacity, 10mL VialsLarge Capacity Tray, 580265

96 Vial Capacity, 1.5mL Vials

3138

ALLTECH 580 AUTOSAMPLER SPECIFICATIONS

Vial Capacity: 84 (1.5mL) vials, 3 (10mL) vials96 (1.5mL) vials (optional),24 (10mL) vials (optional)

Injection Volume: 1-5000µL in 1µL incrementsInjections per Vial: 9, maximumAnalysis Time: 9hrs 59min 59sec, maximumReproducibility: Full Loop Injections: <=0.3% RSD

Partial Loop Injections: <=0.5% RSDµL Pick-Up Injections (>10µL):<=1.0%

Carry Over: <0.05% with needle washColumn Oven Temp. Range: Ambient+5°C - 60°CHeated Zone Dimensions: 1" W x 1" D x 11.8" H

(2.5cm W x 2.5cm D x 30cm H)Power Requirements: 115 VAC; +15% - 22%; 50/60Hz; 100 VA

230 VAC; +15% - 22%; 50/60Hz; 100 VADimensions: 11.8" W x 19.7" D x 13.4" H

(30cm W x 50cm D x 34cm H)Weight: 33lbs (15kgs), 39lbs (18kgs) with cooling option

Need more information on the 580 Autosampler?Request ISS #007

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Documents

Ion Chromatography - Universidad De Antioquiaquimica.udea.edu.co/~carlopez/cromatoion/Ion... · Ez-Lute ™ Buffer ... Ion Chromatography: Alltech vs. EPA Method 300.0 A0037 Analysis