Introduction Of Ddobyul Technology 8.12

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This document is CONFIDENTIAL and the property of Unhwa Corporation. It is intended solely for the use of the individual to whom it is addressed or any other recipient expressly authorized by

Unhwa Corporation. Any unauthorized disclosure, reproduction, copying, distribution or use of this document is strictly

prohibited.

Copyright by 2005-2012 Unhwa Corporation. All rights re-served.

Unhwa CorporationDdobyul Plant Stem Cell Intro-duction

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Power of Plant

Power of Plant

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The oldest living organism

on earth?

Bristlecone Pines in California, U.S.,

which have been alive for 4,772 years.

Power of Plant

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The largest living organism

on earth?

the Giant Sequoia in California, U.S., with 87

meters in height and 2,000 tons in weight

Power of Plant

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Power of Plant

Ginkgo tree is also known as “living fossil”,

as it coexisted with dinosaurs 250 million years ago and

survived through Ice Age with strong vitality.

Adonis annua is a perennial plant which does

not lose its vitality, even blooming flowers breaking

through the snow

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Power of Plant

Endless Living Cycle for Plants

One-way aging process for animals and human beings

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Secret of Plant’s Vitality

Secret of Plant’s Vi-tality

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VASCULARCAMBIUM

VASCULARCAMBIUM

“Plant stem cells, contained in specialized structures called

meristems, have amazing regenerative powers.

They enable plants to grow and produce new organs

throughout lifetimes that can span hundreds of years.”

- ’Nature’ 415, 751-754 (2002)

Plant stem cell is an immortal cell and the origin of plant vitality in which

the growth and regeneration of plant is programmed in its entirety.

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“They enable plants to grow and produce new organs throughout lifetimes that can span hundreds of years.”

- ’Nature’ 415, 751-754 (2002)

- Massive Multiply: exact duplication

- Generate other Plant Organs

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횡단면

Preserve lots of nec-

essary materials for

regenerationPlant Stem Cell

has Great Re-

generation

Power

Prepare for a new lifecycle

-Regeneration Power reason of why trees can grow again in the spring

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- Source of Plant’s Surviving Strategy Plant stem cells make useful compounds for the whole plant to survive in the harsh environment.

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However due to the distinct structural characteristics of plant vascular stem cell, none has succeeded in isolation and culture of plant vascular stem cell (vascular cambium).


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Ddobyul® Plant Stem Cell


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In 2005, Unhwa Corporation succeeded in

the world’s first isolation and culture of plant stem cell.

This pure plant stem cell is trademarked as Ddobyul®.

Following the success of isolating plant stem cell, in 2006,

Unhwa Corporation began joint research with University of

Edinburgh Institute of Molecular Plant Sciences, a world-

renowned biotechnology research institute.

The research outcome of plant stem cell isolation and culture

technology and the potentials of bioprocess innovation were

published by a world-renowned scientific journal, Nature

Biotechnology.

*Ddobyul® is a registered trademark of Unhwa Corporation.

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Published on Top Academic Journal:Nature Biotechnol-ogy2010 November

Platform Technol-ogy Patent Regis-tered in Major Countries

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2011 Korea Technology Award

Green Certification & New Ex-cellent Technology Certifica-tion

Documentaries by Discovery Channel

Coverage by all major media in Korea

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Unhwa Corporation isolates stem cell from various plant species and conducts mass production to develop

pharmaceutical, nutrition, and cosmetic ingredient as well as botanical drug.

“To our knowledge, this is the firs time a stable plant stem cell line has been generated, constituting a significant milestone in biological research. We consider this work to be cutting-edge research with enormous commercial potential.”

-Professor Gary Loake, Institute of Molecular Plant Sciences, University of Edinburgh

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So far, Rare, Precious, or Endangered Plants are NEVER or SELDOM been used for commercial products, although they are believed to have the best efficacy…

50-year-old Wild Ginseng(USD 100,000 / root)

1,000-year-old Yew Tree(Yew Tree lived from Tang-Dynasty)


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Something only for Kings & Queens once per year….

50-year-old Wild Ginseng:

USD 100,000/root

…Now is ready for everybody’s daily consumption

But, we change it all…

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Strong vitality

Anti-oxidation

Anti-inflammation

Anti-aging

Immune enhancement

Anti-cancer?

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Cosmetics Efficacy of Ddobyul® Plant Stem Cell

Effica-cies

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Efficacy of Ddobyul® Plant Stem Cell

Boundless Vital-ity• Ddobyul is pure plant stem cell that does not suffer from variation even after long period of culture. Ddobyul has a strong vitality and survives various physical, chemical, and biological stress.

Long-term culture

Different from common plant cells, Ddobyul maintains the same stable biological and genetic states for long period of culturing.

Initial culture

Callus

Cell

Ddobyul

After 3 years

Survival in Freezing

Culture after Thawing

Callus

Cell

The cell is destroyed by rigid cell wall.

The cell is pre-served by flexible cell wall.

Rapidly dies after thawing.

Re-grows at the same growth rate be-fore freezing.

Ddobyul

Freezing

Different from common plant cells, Ddobyul survives extremely low temperature freez-ing condition, and perfectly re-grows after thawing, which enables the best method for plant cell cryopreservation.

Anti-oxidant Activity

• Generally, the cultured cells have higher anti-oxidant activity compared with other plant cells in order to prevent themselves from being harmed by oxidant stresses of free radicals. Yew Ddobyul has 33.6 times higher CAT* activity than other cultured cell lines.

• Strong anti-oxidant activity keeps the human body healthy by eliminating the free radicals which cause many of the diseases. - Antioxidant efficacy testing with yew Ddobyul -

※ CAT activity is an anti-oxidant activity that serves an important role in eliminat-ing free radicals, and decrease in CAT activity is correlated with progression of Cancer cells (Breast Cancer Research and treatment;2000)

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Anti-cancer Ac-tivity

UHIST proved that yew Ddobyul is non-toxic and has a strong anti-cancer effect through in-vivo & in-vitro experiments per-formed with the advice of Professor Ho-geun Lee of Jeonbuk Uni-versity.

• Anti-cancer in-vivo test w/ yew Ddobyul: Ddobyul treated (oral intake) mice’s tumors weighted approximately 10 times less than the controlled sub-jects. Tumor tissue examination results confirmed that Ddobyul cell line induced cancer cell apoptosis and angiogenesis inhibition.

• Anti-cancer in-vitro test w/ yew Ddobyul: Large intestine cancer, oral cavity carcinoma, lung cancer, prostatic carcinoma, bone cancer, leukemia, uterine carcinoma, skin cancer, pancreas cancer, breast can-cer, stomach cancer, kidney cancer and liver cancer cells were tested dur-ing in-vitro experiment. In comparison to the blockbuster drug ingredient, paclitaxel, Ddobyul cells, which didn’t contain paclitaxel, exhibited simi-lar or more potent anti-cancer capabilities. Although Ddobyul contains multiple compounds, its anti-cancerous suppressive power was no less than paclitaxel, a single substance anti-cancer drug.

Tum

or

vol-

um

e

Con-trol

Ddobyul

Tum

or

weig

ht

Con-trol

Ddobyul

In the experiment to cause inflammatory reaction onto HDF cells applying LPS, yew Ddobyul

methanol extract has shown strong anti-inflammatory activity by inhibiting expression of in-

flammatory cytokines.

* Due to confidentiality, detailed data cannot be disclosed. Should you need additional information, please contact us (see page 17 for contact information).

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Immune Enhancement

Through research collaboration with professor Sang-yun Nam of Jeonju University, Unhwa Biotech Corp. has found that the wild ginseng Ddobyul greatly in-creases the activity of the NK cell*. It also induces bone marrow cell / lymph node cell proliferation and helps boost the overall hematogenous function and immune system.• After injecting an antigen, the mouse was supplemented with Ddobyul in a 1:20 ratio(free-choice feeding) for 10 days. The NK cell activity was ap-proximately 1.5 times greater than the mouse that was not supple-mented with Ddobyul.

• The mice supplemented with feed(free-choice feeding) mixed with Ddobyul had almost twice the number of bone marrow cells com-pared with the mice that were supplemented with only feed. The bone marrow cells produce the main compartments of blood; red blood cells, platelets etc. and can differentiate to immune cells; B cells, T cells etc. The bone marrow cell proliferation signifies the acceleration of the hematogenous function and improvement of the immune system.

• Cells of lymph node increased in the mice that were supplemented with the feed mixed with Ddobyul. Most of cells in the lymph node are T cells and the increase of the cells is the first response to antigen recognition. As antigen-specific T cells increase, other immune responses following T cell-increase are anticipated.

Through collaborative research with a cosmet-ics company with R&D expertise, Unhwa Biotech Corp. has veri-fied that Ddobyul has an outstanding efficacy on the skin. Ddobyul has anti-aging effect to block the very cause of aging. The patent applied on wild ginseng de-

rived plant stem cell’s efficacy of anti-aging and anti-oxidant (Application number : 10-2008-0056008)

The world’s first INCI (the International Nomenclature of Cosmetic Ingredi-ents) registration of plant stem cell and its extract (June 2008)

Anti-aging Activity

Yew tree derived plant stem cell’s efficacy of anti-oxidant and anti-inflammatory (Application number : 10-2008-0044227) Wild ginseng derived plant stem cell’s efficacy of anti-aging and anti-oxidant (Application number : 10-2008-0056008) Yew tree derived plant stem cell’s efficacy of anti-cancer (Application number : 10-2008-0099718) Wild ginseng derived plant stem cell’s efficacy of anti-cancer (Application number : 10-2008-0110086) Wild ginseng derived plant stem cell’s efficacy of immune enhancement (Application number : 10-2009-0015040)

Pat

ents

* Due to confidentiality, detailed data cannot be disclosed. Should you need additional information, please contact us.

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Somatic cell-origin cell culture (A)vs. Ddobyul cell culture (B)

Comparison of competitiveness of Ddobyul cell culture technology

Previous cell culture technology Ddobyul cell culture technology

Genetic variation Variation No variation

Homogeneity Heterogeneous aggregated cell population Homogenous single cell population

Stability of prolifera-tion

Slow cell proliferation in continuous culture, grad-ual loss of cell line proliferation ability

Continuous, stable proliferationwith a high proliferation rate

Productivity of useful material

Variation occurs, loss of productivity during cul-ture

Continuous maintenance of high productivity of useful materials without variation

Cell necrosisCell necrosis from severe aggregation and shear

stressNo cell necrosis from aggregation, strong re-

sistance to shear stress

Need of external ma-terial supply

Requires continuous external material supply No need of continuous material supply

Resistance to Shear stress

Easily damaged/necrotized from stressResistant to stress and shock, proliferation

and productivity are not affected

Survival rate of cell cryopreservation

10~20% 80~90%

A B

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Commercialization of Ddobyul Plant Stem Cell

Ddobyul from various plants possess diverse potentials which directly connect to five different businesses.

Wild Ginseng

Yew tree

…

Various Plant

species

Ddobyul

Efficacy

Anti-cancerAnti-oxidantAnti-agingAnti-inflammatory

Bioprocessinnovation

Stable culture

Cryopreservation

Pharmaceutical

Health food

Cosmetic

Mass production

Cell line bank

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This is the beginning of a New Era• Perfectly Safe• Highly Effective• Stable at Medical Level• No pollution made• Do not harm original plants

Welcome to the revolution brought by Unhwa

Documents

Introduction Of Ddobyul Technology 8.12