Embed Size (px)
Citation preview
Versatile Laboratory
Applications
• Water and wastewater• Food and beverages• Inorganics• Quality control of chemicals• Life science
Visible and UV-Visible Spectrophotometers
Spectronic GENESYS 10
Analyze • Detect • Measure • Control™
Industrial QC
Chemical
Food & Beverage
Environmental
Academic
Scientists, researchers, and techniciansworldwide have long depended on ThermoElectron Corporation to provide Visible andUV-Visible spectrophotometers to suit theirspecific applications and budgets. TheSpectronic GENESYS 10 spectrophotometerseries is the standard that lab managers cancount on to deliver accurate, reliable resultsfor top productivity in the lab.
Meet the New Laboratory Standard
BR50857_E 12/04M
Thermo Electron Scientific Instruments Corporation, Madison, WI USA is ISO Certified.
©2004 Thermo Electron Corporation. All rights reserved.All trademarks are the property of Thermo ElectronCorporation and its subsidiaries.
Specifications, terms and pricing are subject to change.Not all products are available in all countries. Pleaseconsult your local sales representative for details.
In addition to these offices, ThermoElectron Corporation maintains a network of representative organizationsthroughout the world.
Australia+61 2 9898 1244 • [email protected]
Austria+43 1 333 50340 • [email protected]
Belgium+32 2 482 30 30 • [email protected]
Canada+1 800 532 4752 • [email protected]
China+86 10 5850 3588 • [email protected]
France+33 1 60 92 48 00 • [email protected]
Germany+49 6103 4080 • [email protected]
Italy+39 02 950 591 • [email protected]
Japan+81 45 453 9100 • [email protected]
Netherlands+31 76 587 98 88 • [email protected]
Nordic+46 8 556 468 00 • [email protected]
South Africa+27 11 570 1840 • [email protected]
Spain+34 91 657 4930 • [email protected]
Switzerland+41 61 48784 00 • [email protected]
UK+44 1442 233555 • [email protected]
USA+1 800 532 4752 • [email protected]
www.thermo.com
Spectronic Spectronic SpectronicGENESYS 10 Vis GENESYS 10 UV-Vis GENESYS 10 UV-Vis ScanningPart Number 335900 Series Part Number 335902 Series Part Number 335906 Series
Spectral Bandwidth 5 nm
Optical Design Single-beam, grating-based, Split-beam, grating-based, dual detectorssingle detector
Lamp Source Tungsten-Halogen (1000 hrs.) Xenon (5 years)(typical service life)
Wavelength:Range 325 – 1100 nm 190 – 1100 nmAccuracy ± 1.0 nm ± 1.0 nmRepeatability ± 0.5 nm ± 0.5 nm
Display 320 x 240 pixel backlit LCD, 3.8" x 2.8"
Photometric:Range - 0.1 – 3.0 A; 0.3 – 125%T; ± 9999 CReadout Absorbance, Transmittance, ConcentrationAccuracy 0.5% or 0.005 A, whichever is greater, up to 2 A
Noise < 1 mA at 0 A; < 2 mA at 2 A, peak-to-peak at 340 nm
Drift < 2 mA/hour (after warmup) < 1 mA/hour
Stray Light < 0.1%T at 340 and 400 nm < 0.1%T at 220, 340, and 400 nm
Data Interface Bi-directional RS232C Bi-directional RS232C (text only); parallel(text only) output in HP PCL format (text and graphics)
Cell Holder 1-position or automatic 6-position Cell Holder
Keyboard Membrane keypad
Software
Test Storage Up to 80 sets of test parameters
Languages Software, printouts and operator’s manual: English, French, German, Spanish, Italian (user selectable)
Internal Printer 40-column internal graphics(optional)
Analog Output 0 – 1 V for - 0.3 to 2.5 A Not available(optional)
Power Requirements Selected automatically; 100 – 240 Volts
Dimensions 300 W x 400 D x 250 H mm (11.8" x 15.7" x 9.8")
Weight 8.6 kg (19 lb.)
Warranty 1 year
Absorbance/Transmittance/ConcentrationStandard Curve Absorbance RatioAbsorbance DifferenceKineticsSurvey Scan (100 nm increments)3-Point NetMultiwavelengthPerformance Validation
Same as Spectronic GENESYS 10 UV-Visbut with full-range scanningScanning Speed: 200 – 1000 nm/minSampling Interval: 1, 2, 3, 5 nmCell CorrectionSmartStart Program
m o l e c u l a r s p e c t r o s c o p y
Versatile Laboratory
Applications
• Water and wastewater• Food and beverages• Inorganics• Quality control of chemicals• Life science
Visible and UV-Visible Spectrophotometers
Spectronic GENESYS 10
Analyze • Detect • Measure • Control™
Industrial QC
Chemical
Food & Beverage
Environmental
Academic
Scientists, researchers, and techniciansworldwide have long depended on ThermoElectron Corporation to provide Visible andUV-Visible spectrophotometers to suit theirspecific applications and budgets. TheSpectronic GENESYS 10 spectrophotometerseries is the standard that lab managers cancount on to deliver accurate, reliable resultsfor top productivity in the lab.
Meet the New Laboratory Standard
BR50857_E 12/04M
Thermo Electron Scientific Instruments Corporation, Madison, WI USA is ISO Certified.
©2004 Thermo Electron Corporation. All rights reserved.All trademarks are the property of Thermo ElectronCorporation and its subsidiaries.
Specifications, terms and pricing are subject to change.Not all products are available in all countries. Pleaseconsult your local sales representative for details.
In addition to these offices, ThermoElectron Corporation maintains a network of representative organizationsthroughout the world.
Australia+61 2 9898 1244 • [email protected]
Austria+43 1 333 50340 • [email protected]
Belgium+32 2 482 30 30 • [email protected]
Canada+1 800 532 4752 • [email protected]
China+86 10 5850 3588 • [email protected]
France+33 1 60 92 48 00 • [email protected]
Germany+49 6103 4080 • [email protected]
Italy+39 02 950 591 • [email protected]
Japan+81 45 453 9100 • [email protected]
Netherlands+31 76 587 98 88 • [email protected]
Nordic+46 8 556 468 00 • [email protected]
South Africa+27 11 570 1840 • [email protected]
Spain+34 91 657 4930 • [email protected]
Switzerland+41 61 48784 00 • [email protected]
UK+44 1442 233555 • [email protected]
USA+1 800 532 4752 • [email protected]
www.thermo.com
Spectronic Spectronic SpectronicGENESYS 10 Vis GENESYS 10 UV-Vis GENESYS 10 UV-Vis ScanningPart Number 335900 Series Part Number 335902 Series Part Number 335906 Series
Spectral Bandwidth 5 nm
Optical Design Single-beam, grating-based, Split-beam, grating-based, dual detectorssingle detector
Lamp Source Tungsten-Halogen (1000 hrs.) Xenon (5 years)(typical service life)
Wavelength:Range 325 – 1100 nm 190 – 1100 nmAccuracy ± 1.0 nm ± 1.0 nmRepeatability ± 0.5 nm ± 0.5 nm
Display 320 x 240 pixel backlit LCD, 3.8" x 2.8"
Photometric:Range - 0.1 – 3.0 A; 0.3 – 125%T; ± 9999 CReadout Absorbance, Transmittance, ConcentrationAccuracy 0.5% or 0.005 A, whichever is greater, up to 2 A
Noise < 1 mA at 0 A; < 2 mA at 2 A, peak-to-peak at 340 nm
Drift < 2 mA/hour (after warmup) < 1 mA/hour
Stray Light < 0.1%T at 340 and 400 nm < 0.1%T at 220, 340, and 400 nm
Data Interface Bi-directional RS232C Bi-directional RS232C (text only); parallel(text only) output in HP PCL format (text and graphics)
Cell Holder 1-position or automatic 6-position Cell Holder
Keyboard Membrane keypad
Software
Test Storage Up to 80 sets of test parameters
Languages Software, printouts and operator’s manual: English, French, German, Spanish, Italian (user selectable)
Internal Printer 40-column internal graphics(optional)
Analog Output 0 – 1 V for - 0.3 to 2.5 A Not available(optional)
Power Requirements Selected automatically; 100 – 240 Volts
Dimensions 300 W x 400 D x 250 H mm (11.8" x 15.7" x 9.8")
Weight 8.6 kg (19 lb.)
Warranty 1 year
Absorbance/Transmittance/ConcentrationStandard Curve Absorbance RatioAbsorbance DifferenceKineticsSurvey Scan (100 nm increments)3-Point NetMultiwavelengthPerformance Validation
Same as Spectronic GENESYS 10 UV-Visbut with full-range scanningScanning Speed: 200 – 1000 nm/minSampling Interval: 1, 2, 3, 5 nmCell CorrectionSmartStart Program
m o l e c u l a r s p e c t r o s c o p y
Versatile Laboratory
Applications
• Water and wastewater• Food and beverages• Inorganics• Quality control of chemicals• Life science
Visible and UV-Visible Spectrophotometers
Spectronic GENESYS 10
Analyze • Detect • Measure • Control™
Industrial QC
Chemical
Food & Beverage
Environmental
Academic
Scientists, researchers, and techniciansworldwide have long depended on ThermoElectron Corporation to provide Visible andUV-Visible spectrophotometers to suit theirspecific applications and budgets. TheSpectronic GENESYS 10 spectrophotometerseries is the standard that lab managers cancount on to deliver accurate, reliable resultsfor top productivity in the lab.
Meet the New Laboratory Standard
BR50857_E 12/04M
Thermo Electron Scientific Instruments Corporation, Madison, WI USA is ISO Certified.
©2004 Thermo Electron Corporation. All rights reserved.All trademarks are the property of Thermo ElectronCorporation and its subsidiaries.
Specifications, terms and pricing are subject to change.Not all products are available in all countries. Pleaseconsult your local sales representative for details.
In addition to these offices, ThermoElectron Corporation maintains a network of representative organizationsthroughout the world.
Australia+61 2 9898 1244 • [email protected]
Austria+43 1 333 50340 • [email protected]
Belgium+32 2 482 30 30 • [email protected]
Canada+1 800 532 4752 • [email protected]
China+86 10 5850 3588 • [email protected]
France+33 1 60 92 48 00 • [email protected]
Germany+49 6103 4080 • [email protected]
Italy+39 02 950 591 • [email protected]
Japan+81 45 453 9100 • [email protected]
Netherlands+31 76 587 98 88 • [email protected]
Nordic+46 8 556 468 00 • [email protected]
South Africa+27 11 570 1840 • [email protected]
Spain+34 91 657 4930 • [email protected]
Switzerland+41 61 48784 00 • [email protected]
UK+44 1442 233555 • [email protected]
USA+1 800 532 4752 • [email protected]
www.thermo.com
Spectronic Spectronic SpectronicGENESYS 10 Vis GENESYS 10 UV-Vis GENESYS 10 UV-Vis ScanningPart Number 335900 Series Part Number 335902 Series Part Number 335906 Series
Spectral Bandwidth 5 nm
Optical Design Single-beam, grating-based, Split-beam, grating-based, dual detectorssingle detector
Lamp Source Tungsten-Halogen (1000 hrs.) Xenon (5 years)(typical service life)
Wavelength:Range 325 – 1100 nm 190 – 1100 nmAccuracy ± 1.0 nm ± 1.0 nmRepeatability ± 0.5 nm ± 0.5 nm
Display 320 x 240 pixel backlit LCD, 3.8" x 2.8"
Photometric:Range - 0.1 – 3.0 A; 0.3 – 125%T; ± 9999 CReadout Absorbance, Transmittance, ConcentrationAccuracy 0.5% or 0.005 A, whichever is greater, up to 2 A
Noise < 1 mA at 0 A; < 2 mA at 2 A, peak-to-peak at 340 nm
Drift < 2 mA/hour (after warmup) < 1 mA/hour
Stray Light < 0.1%T at 340 and 400 nm < 0.1%T at 220, 340, and 400 nm
Data Interface Bi-directional RS232C Bi-directional RS232C (text only); parallel(text only) output in HP PCL format (text and graphics)
Cell Holder 1-position or automatic 6-position Cell Holder
Keyboard Membrane keypad
Software
Test Storage Up to 80 sets of test parameters
Languages Software, printouts and operator’s manual: English, French, German, Spanish, Italian (user selectable)
Internal Printer 40-column internal graphics(optional)
Analog Output 0 – 1 V for - 0.3 to 2.5 A Not available(optional)
Power Requirements Selected automatically; 100 – 240 Volts
Dimensions 300 W x 400 D x 250 H mm (11.8" x 15.7" x 9.8")
Weight 8.6 kg (19 lb.)
Warranty 1 year
Absorbance/Transmittance/ConcentrationStandard Curve Absorbance RatioAbsorbance DifferenceKineticsSurvey Scan (100 nm increments)3-Point NetMultiwavelengthPerformance Validation
Same as Spectronic GENESYS 10 UV-Visbut with full-range scanningScanning Speed: 200 – 1000 nm/minSampling Interval: 1, 2, 3, 5 nmCell CorrectionSmartStart Program
m o l e c u l a r s p e c t r o s c o p y
A powerful, long-lastingxenon lamp provides high-energy light throughput foraccuracy and precision –and runs for years withoutreplacement.
A large graphical display allows easyviewing of instrument conditions and results.
The small instrument footprintgives you plenty of room forother lab equipment.
Cleanable, chemical-resistant surfaces andrugged design make this system the idealchoice for harsh laboratory environments.
Simple keyboard-based operation and “Softkeys”, specific to each screen, simplify setting up the instrument with your laboratory’s test parameters.
6-position Cell Holder models hold a blank and up to five samples in 10 mm square cuvettes, or 10 mm or 1/2" test tubes. The standard turret will measure all six positionsautomatically or can be rotated tospecific positions by pressing thecell changer buttons. The powerfuloptics allow you to measure 70 µLor 10 µL samples in the single cell holder.
An optional, built-in, 3 1/8" (79 mm)wide printer documents all parametersincluding:
• time & date• instrument serial number• test name• test data• final test results
It displays graphs, statistics, and sample number – all useful for GoodLaboratory Practice (GLP) requirements.
Cell changer buttons rotatesamples into position.
Full-range scanscan be run on theSpectronicGENESYS 10 UV-Visscanning model.
The Spectronic GENESYS 10 spectrophotometer series is ready tomeet the demands of today’s busy labs. These instruments have allof the features you have come to expect from a Spectronic GENESYSspectrophotometer including a rugged design, a user-friendly interfaceand unrivaled reliability. Best of all, this spectrophotometer seriesprovides the functionality of an advanced instrument, yet is affordablypriced. All Visible and UV-Visible Spectronic GENESYS 10spectrophotometer models feature a powerful optical design, flexible sample handling and versatile software capabilities thatmake these systems the best choice for your application needs.
Powered to Perform, Priced to Surprise Unique Benefits Save Time and Money
With the UV-Vis scanning model, save time by using the handy SmartStart™ feature toselect specific tests to appear on the screen at instrument boot-up.
UV-Vis scanning model tests also include an exclusive Cell Correction program. It“matches” up to six cuvettes for up to 31 individual wavelengths AND one full wavelengthscan, eliminating the need to purchase expensive matched cuvettes.
Complex Tests Made Easy
The Spectronic GENESYS 10 spectrophotometer has been programmed to perform complextests as effortlessly as your simple measurements. As you begin your tests, the instrumentinstantly zeroes your blank and displays each cell measuring position throughout the test.
All test programs allow you to set the measurement mode, run single or multiple* samples, print automatically, and perform statistics on the final results. Program the instrument once with each of our customized tests, name them, and they will always beavailable for quick and easy recall. A total of 80 tests can be saved, with the option to locktests for security.
* Scan and kinetics tests allow single samples only.
Standard Curve
• Shows standard curve with date prepared• Offers seven standard-curve fit options• Displays data in any of 10 pre-programmed units or allows you
to create your own• Allows measurement of up to 15 standards
(a) Set-up screen for preparing standard curve showing curve fit options
(b) Measurement of sulfide standards showing absorbances and statistical values
Absorbance Difference
• Optional 3rd wavelength for turbidity correction• Programmable factors to calculate corrected protein and nucleic
acid concentration• Formula: (Aλ1)factor1 – (Aλ2)factor2
OR(Aλ1 – Aref)factor1 – (Aλ2 – Aref)factor2
(d) Calculation of protein concentration in Absorbance Difference modeusing Warburg-Christian equation
Absorbance Ratio
• Calculates the absorbance ratio of two wavelengths with anoptional reference wavelength
• Formula:
(e) Results of DNA purity measurement showing test name, absorbance values and outside-range indication
ORAbsλ1
Absλ2
Absλ1 – Absref
Absλ2 – Absref
(c)
(a)
(b)
(d)
(e)
Multiwavelength
• Measure absorbances of up to 31 wavelengths on one sample• Calculate concentration at each wavelength
(c) Measurement of multiple wavelengths at 10 nm increments
Scan• Scan at different scan speeds• Label peaks and valleys – select specific wavelengths to report
values for QC applications• Expand scale• Calculate 3-point net and area under the curve
(h) Scan of DNA showing data presentation and editing options
(i) Screen showing calculation of 3-point net value
Kinetics• Measure reactions in intervals as low as one second• Measure reactions for up to 96 hours• Calculate reaction rates and display results in units of your choice• Rescale to get rates over linear phases of the reaction• View on screen or on optional analog recorder
(j) Enzyme kinetics run showing curve and concentration calculation
3-Point Net• 3-Point Net method measures at three-entered wavelengths to
compensate for a sloping baseline caused by turbidity
• Formula:
Performance Validation• Perform routine performance checks on your instrument• Time, date, serial number shown on printout• Verify the document instrument performance to satisfy regulatory
requirements• Use with Spectronic Standards for performance validation• Wavelength Accuracy: Uses internal lamp or filter standards; Visible
model has built-in didymium filter; UV-Visible models use multiplewavelengths on the xenon lamp
• Photometric Accuracy: Use Spectronic Standards or third-party standards. Set tolerances to flag out-of-range results.
• Noise: Measurements at 0 A done at 230, 340 or 500 nm. Set yourown tolerances; see actual results with Pass/Fail indication
• Stray Radiant Energy: Measure stray light of optical system at 220,340 and 400 nm; reports Pass/Fail result
• Diagnostics: Check internal printer, RS232C and Analog Output
(f) Selection of tests in Performance Validation program
(g) Screen used to enter desired wavelength accuracy tolerance
(f)
(g)
(h)
(i)
(j)
Printout from built-in printer of sulfidedetermination showing test parameters,standard curve graph, statistics andabsorbance values
Spectronic GENESYS 10 Vis
Spectronic GENESYS 10 UV-Vis
Spectronic GENESYS 10 UV-Vis Scanning
Routine Absorbance andTransmittance measurementscan be performed from the instrument’s main screen using a very simple three-step process.Concentration measurementsonly require two additional steps:measuring and entering the concentration of the standard.
λ3 – λ2
λ3 – λ1
A2 – A3 +([A1 – A3] * )( )
Flow-Through Accessory
• Optical glass or quartz flowcell
• Great for speeding up sample throughput
• Selectable tubing and pumping times accommodate sample volumes from 2 – 15 mL
• Option to return sample to vessel
Filter Holders
• Used to measure transmittance and cut-off wavelengths in glass and filters
• Both spring-loaded and adjustable holders are available
Test Tube Holder
• Ideal for COD tubes and glassware fromreagent kits
• Holds test tubes up to 25 mm diameter and up to 102 mm tall
Funnel Flowcell
• Fast sample throughput
• Used with external vacuum pump orwater aspiration system
• Used on Single Cell Platform
Longpath Cell Holders
• Used to increase sensitivity of readings
• Hold rectangular or cylindrical cells
• Hold up to three 50 mm pathlength cells in 6-Position Cell Holder or one 100 mm pathlengthcell in Single Cell Platform
Faster Measurements Using
Sample Holder of Your Choice
Before the software can work its magic, you need aninstrument versatile enough to accommodate a widerange of sample holders. The Spectronic GENESYS 10spectrophotometer gives you an impressive range ofchoices for fast, efficient sample measurements.
Typical printout showing wavelength
accuracy, photometric accuracy, noise and stray light measurements
A powerful, long-lastingxenon lamp provides high-energy light throughput foraccuracy and precision –and runs for years withoutreplacement.
A large graphical display allows easyviewing of instrument conditions and results.
The small instrument footprintgives you plenty of room forother lab equipment.
Cleanable, chemical-resistant surfaces andrugged design make this system the idealchoice for harsh laboratory environments.
Simple keyboard-based operation and “Softkeys”, specific to each screen, simplify setting up the instrument with your laboratory’s test parameters.
6-position Cell Holder models hold a blank and up to five samples in 10 mm square cuvettes, or 10 mm or 1/2" test tubes. The standard turret will measure all six positionsautomatically or can be rotated tospecific positions by pressing thecell changer buttons. The powerfuloptics allow you to measure 70 µLor 10 µL samples in the single cell holder.
An optional, built-in, 3 1/8" (79 mm)wide printer documents all parametersincluding:
• time & date• instrument serial number• test name• test data• final test results
It displays graphs, statistics, and sample number – all useful for GoodLaboratory Practice (GLP) requirements.
Cell changer buttons rotatesamples into position.
Full-range scanscan be run on theSpectronicGENESYS 10 UV-Visscanning model.
The Spectronic GENESYS 10 spectrophotometer series is ready tomeet the demands of today’s busy labs. These instruments have allof the features you have come to expect from a Spectronic GENESYSspectrophotometer including a rugged design, a user-friendly interfaceand unrivaled reliability. Best of all, this spectrophotometer seriesprovides the functionality of an advanced instrument, yet is affordablypriced. All Visible and UV-Visible Spectronic GENESYS 10spectrophotometer models feature a powerful optical design, flexible sample handling and versatile software capabilities thatmake these systems the best choice for your application needs.
Powered to Perform, Priced to Surprise Unique Benefits Save Time and Money
With the UV-Vis scanning model, save time by using the handy SmartStart™ feature toselect specific tests to appear on the screen at instrument boot-up.
UV-Vis scanning model tests also include an exclusive Cell Correction program. It“matches” up to six cuvettes for up to 31 individual wavelengths AND one full wavelengthscan, eliminating the need to purchase expensive matched cuvettes.
Complex Tests Made Easy
The Spectronic GENESYS 10 spectrophotometer has been programmed to perform complextests as effortlessly as your simple measurements. As you begin your tests, the instrumentinstantly zeroes your blank and displays each cell measuring position throughout the test.
All test programs allow you to set the measurement mode, run single or multiple* samples, print automatically, and perform statistics on the final results. Program the instrument once with each of our customized tests, name them, and they will always beavailable for quick and easy recall. A total of 80 tests can be saved, with the option to locktests for security.
* Scan and kinetics tests allow single samples only.
Standard Curve
• Shows standard curve with date prepared• Offers seven standard-curve fit options• Displays data in any of 10 pre-programmed units or allows you
to create your own• Allows measurement of up to 15 standards
(a) Set-up screen for preparing standard curve showing curve fit options
(b) Measurement of sulfide standards showing absorbances and statistical values
Absorbance Difference
• Optional 3rd wavelength for turbidity correction• Programmable factors to calculate corrected protein and nucleic
acid concentration• Formula: (Aλ1)factor1 – (Aλ2)factor2
OR(Aλ1 – Aref)factor1 – (Aλ2 – Aref)factor2
(d) Calculation of protein concentration in Absorbance Difference modeusing Warburg-Christian equation
Absorbance Ratio
• Calculates the absorbance ratio of two wavelengths with anoptional reference wavelength
• Formula:
(e) Results of DNA purity measurement showing test name, absorbance values and outside-range indication
ORAbsλ1
Absλ2
Absλ1 – Absref
Absλ2 – Absref
(c)
(a)
(b)
(d)
(e)
Multiwavelength
• Measure absorbances of up to 31 wavelengths on one sample• Calculate concentration at each wavelength
(c) Measurement of multiple wavelengths at 10 nm increments
Scan• Scan at different scan speeds• Label peaks and valleys – select specific wavelengths to report
values for QC applications• Expand scale• Calculate 3-point net and area under the curve
(h) Scan of DNA showing data presentation and editing options
(i) Screen showing calculation of 3-point net value
Kinetics• Measure reactions in intervals as low as one second• Measure reactions for up to 96 hours• Calculate reaction rates and display results in units of your choice• Rescale to get rates over linear phases of the reaction• View on screen or on optional analog recorder
(j) Enzyme kinetics run showing curve and concentration calculation
3-Point Net• 3-Point Net method measures at three-entered wavelengths to
compensate for a sloping baseline caused by turbidity
• Formula:
Performance Validation• Perform routine performance checks on your instrument• Time, date, serial number shown on printout• Verify the document instrument performance to satisfy regulatory
requirements• Use with Spectronic Standards for performance validation• Wavelength Accuracy: Uses internal lamp or filter standards; Visible
model has built-in didymium filter; UV-Visible models use multiplewavelengths on the xenon lamp
• Photometric Accuracy: Use Spectronic Standards or third-party standards. Set tolerances to flag out-of-range results.
• Noise: Measurements at 0 A done at 230, 340 or 500 nm. Set yourown tolerances; see actual results with Pass/Fail indication
• Stray Radiant Energy: Measure stray light of optical system at 220,340 and 400 nm; reports Pass/Fail result
• Diagnostics: Check internal printer, RS232C and Analog Output
(f) Selection of tests in Performance Validation program
(g) Screen used to enter desired wavelength accuracy tolerance
(f)
(g)
(h)
(i)
(j)
Printout from built-in printer of sulfidedetermination showing test parameters,standard curve graph, statistics andabsorbance values
Spectronic GENESYS 10 Vis
Spectronic GENESYS 10 UV-Vis
Spectronic GENESYS 10 UV-Vis Scanning
Routine Absorbance andTransmittance measurementscan be performed from the instrument’s main screen using a very simple three-step process.Concentration measurementsonly require two additional steps:measuring and entering the concentration of the standard.
λ3 – λ2
λ3 – λ1
A2 – A3 +([A1 – A3] * )( )
Flow-Through Accessory
• Optical glass or quartz flowcell
• Great for speeding up sample throughput
• Selectable tubing and pumping times accommodate sample volumes from 2 – 15 mL
• Option to return sample to vessel
Filter Holders
• Used to measure transmittance and cut-off wavelengths in glass and filters
• Both spring-loaded and adjustable holders are available
Test Tube Holder
• Ideal for COD tubes and glassware fromreagent kits
• Holds test tubes up to 25 mm diameter and up to 102 mm tall
Funnel Flowcell
• Fast sample throughput
• Used with external vacuum pump orwater aspiration system
• Used on Single Cell Platform
Longpath Cell Holders
• Used to increase sensitivity of readings
• Hold rectangular or cylindrical cells
• Hold up to three 50 mm pathlength cells in 6-Position Cell Holder or one 100 mm pathlengthcell in Single Cell Platform
Faster Measurements Using
Sample Holder of Your Choice
Before the software can work its magic, you need aninstrument versatile enough to accommodate a widerange of sample holders. The Spectronic GENESYS 10spectrophotometer gives you an impressive range ofchoices for fast, efficient sample measurements.
Typical printout showing wavelength
accuracy, photometric accuracy, noise and stray light measurements
A powerful, long-lastingxenon lamp provides high-energy light throughput foraccuracy and precision –and runs for years withoutreplacement.
A large graphical display allows easyviewing of instrument conditions and results.
The small instrument footprintgives you plenty of room forother lab equipment.
Cleanable, chemical-resistant surfaces andrugged design make this system the idealchoice for harsh laboratory environments.
Simple keyboard-based operation and “Softkeys”, specific to each screen, simplify setting up the instrument with your laboratory’s test parameters.
6-position Cell Holder models hold a blank and up to five samples in 10 mm square cuvettes, or 10 mm or 1/2" test tubes. The standard turret will measure all six positionsautomatically or can be rotated tospecific positions by pressing thecell changer buttons. The powerfuloptics allow you to measure 70 µLor 10 µL samples in the single cell holder.
An optional, built-in, 3 1/8" (79 mm)wide printer documents all parametersincluding:
• time & date• instrument serial number• test name• test data• final test results
It displays graphs, statistics, and sample number – all useful for GoodLaboratory Practice (GLP) requirements.
Cell changer buttons rotatesamples into position.
Full-range scanscan be run on theSpectronicGENESYS 10 UV-Visscanning model.
The Spectronic GENESYS 10 spectrophotometer series is ready tomeet the demands of today’s busy labs. These instruments have allof the features you have come to expect from a Spectronic GENESYSspectrophotometer including a rugged design, a user-friendly interfaceand unrivaled reliability. Best of all, this spectrophotometer seriesprovides the functionality of an advanced instrument, yet is affordablypriced. All Visible and UV-Visible Spectronic GENESYS 10spectrophotometer models feature a powerful optical design, flexible sample handling and versatile software capabilities thatmake these systems the best choice for your application needs.
Powered to Perform, Priced to Surprise Unique Benefits Save Time and Money
With the UV-Vis scanning model, save time by using the handy SmartStart™ feature toselect specific tests to appear on the screen at instrument boot-up.
UV-Vis scanning model tests also include an exclusive Cell Correction program. It“matches” up to six cuvettes for up to 31 individual wavelengths AND one full wavelengthscan, eliminating the need to purchase expensive matched cuvettes.
Complex Tests Made Easy
The Spectronic GENESYS 10 spectrophotometer has been programmed to perform complextests as effortlessly as your simple measurements. As you begin your tests, the instrumentinstantly zeroes your blank and displays each cell measuring position throughout the test.
All test programs allow you to set the measurement mode, run single or multiple* samples, print automatically, and perform statistics on the final results. Program the instrument once with each of our customized tests, name them, and they will always beavailable for quick and easy recall. A total of 80 tests can be saved, with the option to locktests for security.
* Scan and kinetics tests allow single samples only.
Standard Curve
• Shows standard curve with date prepared• Offers seven standard-curve fit options• Displays data in any of 10 pre-programmed units or allows you
to create your own• Allows measurement of up to 15 standards
(a) Set-up screen for preparing standard curve showing curve fit options
(b) Measurement of sulfide standards showing absorbances and statistical values
Absorbance Difference
• Optional 3rd wavelength for turbidity correction• Programmable factors to calculate corrected protein and nucleic
acid concentration• Formula: (Aλ1)factor1 – (Aλ2)factor2
OR(Aλ1 – Aref)factor1 – (Aλ2 – Aref)factor2
(d) Calculation of protein concentration in Absorbance Difference modeusing Warburg-Christian equation
Absorbance Ratio
• Calculates the absorbance ratio of two wavelengths with anoptional reference wavelength
• Formula:
(e) Results of DNA purity measurement showing test name, absorbance values and outside-range indication
ORAbsλ1
Absλ2
Absλ1 – Absref
Absλ2 – Absref
(c)
(a)
(b)
(d)
(e)
Multiwavelength
• Measure absorbances of up to 31 wavelengths on one sample• Calculate concentration at each wavelength
(c) Measurement of multiple wavelengths at 10 nm increments
Scan• Scan at different scan speeds• Label peaks and valleys – select specific wavelengths to report
values for QC applications• Expand scale• Calculate 3-point net and area under the curve
(h) Scan of DNA showing data presentation and editing options
(i) Screen showing calculation of 3-point net value
Kinetics• Measure reactions in intervals as low as one second• Measure reactions for up to 96 hours• Calculate reaction rates and display results in units of your choice• Rescale to get rates over linear phases of the reaction• View on screen or on optional analog recorder
(j) Enzyme kinetics run showing curve and concentration calculation
3-Point Net• 3-Point Net method measures at three-entered wavelengths to
compensate for a sloping baseline caused by turbidity
• Formula:
Performance Validation• Perform routine performance checks on your instrument• Time, date, serial number shown on printout• Verify the document instrument performance to satisfy regulatory
requirements• Use with Spectronic Standards for performance validation• Wavelength Accuracy: Uses internal lamp or filter standards; Visible
model has built-in didymium filter; UV-Visible models use multiplewavelengths on the xenon lamp
• Photometric Accuracy: Use Spectronic Standards or third-party standards. Set tolerances to flag out-of-range results.
• Noise: Measurements at 0 A done at 230, 340 or 500 nm. Set yourown tolerances; see actual results with Pass/Fail indication
• Stray Radiant Energy: Measure stray light of optical system at 220,340 and 400 nm; reports Pass/Fail result
• Diagnostics: Check internal printer, RS232C and Analog Output
(f) Selection of tests in Performance Validation program
(g) Screen used to enter desired wavelength accuracy tolerance
(f)
(g)
(h)
(i)
(j)
Printout from built-in printer of sulfidedetermination showing test parameters,standard curve graph, statistics andabsorbance values
Spectronic GENESYS 10 Vis
Spectronic GENESYS 10 UV-Vis
Spectronic GENESYS 10 UV-Vis Scanning
Routine Absorbance andTransmittance measurementscan be performed from the instrument’s main screen using a very simple three-step process.Concentration measurementsonly require two additional steps:measuring and entering the concentration of the standard.
λ3 – λ2
λ3 – λ1
A2 – A3 +([A1 – A3] * )( )
Flow-Through Accessory
• Optical glass or quartz flowcell
• Great for speeding up sample throughput
• Selectable tubing and pumping times accommodate sample volumes from 2 – 15 mL
• Option to return sample to vessel
Filter Holders
• Used to measure transmittance and cut-off wavelengths in glass and filters
• Both spring-loaded and adjustable holders are available
Test Tube Holder
• Ideal for COD tubes and glassware fromreagent kits
• Holds test tubes up to 25 mm diameter and up to 102 mm tall
Funnel Flowcell
• Fast sample throughput
• Used with external vacuum pump orwater aspiration system
• Used on Single Cell Platform
Longpath Cell Holders
• Used to increase sensitivity of readings
• Hold rectangular or cylindrical cells
• Hold up to three 50 mm pathlength cells in 6-Position Cell Holder or one 100 mm pathlengthcell in Single Cell Platform
Faster Measurements Using
Sample Holder of Your Choice
Before the software can work its magic, you need aninstrument versatile enough to accommodate a widerange of sample holders. The Spectronic GENESYS 10spectrophotometer gives you an impressive range ofchoices for fast, efficient sample measurements.
Typical printout showing wavelength
accuracy, photometric accuracy, noise and stray light measurements
A powerful, long-lastingxenon lamp provides high-energy light throughput foraccuracy and precision –and runs for years withoutreplacement.
A large graphical display allows easyviewing of instrument conditions and results.
The small instrument footprintgives you plenty of room forother lab equipment.
Cleanable, chemical-resistant surfaces andrugged design make this system the idealchoice for harsh laboratory environments.
Simple keyboard-based operation and “Softkeys”, specific to each screen, simplify setting up the instrument with your laboratory’s test parameters.
6-position Cell Holder models hold a blank and up to five samples in 10 mm square cuvettes, or 10 mm or 1/2" test tubes. The standard turret will measure all six positionsautomatically or can be rotated tospecific positions by pressing thecell changer buttons. The powerfuloptics allow you to measure 70 µLor 10 µL samples in the single cell holder.
An optional, built-in, 3 1/8" (79 mm)wide printer documents all parametersincluding:
• time & date• instrument serial number• test name• test data• final test results
It displays graphs, statistics, and sample number – all useful for GoodLaboratory Practice (GLP) requirements.
Cell changer buttons rotatesamples into position.
Full-range scanscan be run on theSpectronicGENESYS 10 UV-Visscanning model.
The Spectronic GENESYS 10 spectrophotometer series is ready tomeet the demands of today’s busy labs. These instruments have allof the features you have come to expect from a Spectronic GENESYSspectrophotometer including a rugged design, a user-friendly interfaceand unrivaled reliability. Best of all, this spectrophotometer seriesprovides the functionality of an advanced instrument, yet is affordablypriced. All Visible and UV-Visible Spectronic GENESYS 10spectrophotometer models feature a powerful optical design, flexible sample handling and versatile software capabilities thatmake these systems the best choice for your application needs.
Powered to Perform, Priced to Surprise Unique Benefits Save Time and Money
With the UV-Vis scanning model, save time by using the handy SmartStart™ feature toselect specific tests to appear on the screen at instrument boot-up.
UV-Vis scanning model tests also include an exclusive Cell Correction program. It“matches” up to six cuvettes for up to 31 individual wavelengths AND one full wavelengthscan, eliminating the need to purchase expensive matched cuvettes.
Complex Tests Made Easy
The Spectronic GENESYS 10 spectrophotometer has been programmed to perform complextests as effortlessly as your simple measurements. As you begin your tests, the instrumentinstantly zeroes your blank and displays each cell measuring position throughout the test.
All test programs allow you to set the measurement mode, run single or multiple* samples, print automatically, and perform statistics on the final results. Program the instrument once with each of our customized tests, name them, and they will always beavailable for quick and easy recall. A total of 80 tests can be saved, with the option to locktests for security.
* Scan and kinetics tests allow single samples only.
Standard Curve
• Shows standard curve with date prepared• Offers seven standard-curve fit options• Displays data in any of 10 pre-programmed units or allows you
to create your own• Allows measurement of up to 15 standards
(a) Set-up screen for preparing standard curve showing curve fit options
(b) Measurement of sulfide standards showing absorbances and statistical values
Absorbance Difference
• Optional 3rd wavelength for turbidity correction• Programmable factors to calculate corrected protein and nucleic
acid concentration• Formula: (Aλ1)factor1 – (Aλ2)factor2
OR(Aλ1 – Aref)factor1 – (Aλ2 – Aref)factor2
(d) Calculation of protein concentration in Absorbance Difference modeusing Warburg-Christian equation
Absorbance Ratio
• Calculates the absorbance ratio of two wavelengths with anoptional reference wavelength
• Formula:
(e) Results of DNA purity measurement showing test name, absorbance values and outside-range indication
ORAbsλ1
Absλ2
Absλ1 – Absref
Absλ2 – Absref
(c)
(a)
(b)
(d)
(e)
Multiwavelength
• Measure absorbances of up to 31 wavelengths on one sample• Calculate concentration at each wavelength
(c) Measurement of multiple wavelengths at 10 nm increments
Scan• Scan at different scan speeds• Label peaks and valleys – select specific wavelengths to report
values for QC applications• Expand scale• Calculate 3-point net and area under the curve
(h) Scan of DNA showing data presentation and editing options
(i) Screen showing calculation of 3-point net value
Kinetics• Measure reactions in intervals as low as one second• Measure reactions for up to 96 hours• Calculate reaction rates and display results in units of your choice• Rescale to get rates over linear phases of the reaction• View on screen or on optional analog recorder
(j) Enzyme kinetics run showing curve and concentration calculation
3-Point Net• 3-Point Net method measures at three-entered wavelengths to
compensate for a sloping baseline caused by turbidity
• Formula:
Performance Validation• Perform routine performance checks on your instrument• Time, date, serial number shown on printout• Verify the document instrument performance to satisfy regulatory
requirements• Use with Spectronic Standards for performance validation• Wavelength Accuracy: Uses internal lamp or filter standards; Visible
model has built-in didymium filter; UV-Visible models use multiplewavelengths on the xenon lamp
• Photometric Accuracy: Use Spectronic Standards or third-party standards. Set tolerances to flag out-of-range results.
• Noise: Measurements at 0 A done at 230, 340 or 500 nm. Set yourown tolerances; see actual results with Pass/Fail indication
• Stray Radiant Energy: Measure stray light of optical system at 220,340 and 400 nm; reports Pass/Fail result
• Diagnostics: Check internal printer, RS232C and Analog Output
(f) Selection of tests in Performance Validation program
(g) Screen used to enter desired wavelength accuracy tolerance
(f)
(g)
(h)
(i)
(j)
Printout from built-in printer of sulfidedetermination showing test parameters,standard curve graph, statistics andabsorbance values
Spectronic GENESYS 10 Vis
Spectronic GENESYS 10 UV-Vis
Spectronic GENESYS 10 UV-Vis Scanning
Routine Absorbance andTransmittance measurementscan be performed from the instrument’s main screen using a very simple three-step process.Concentration measurementsonly require two additional steps:measuring and entering the concentration of the standard.
λ3 – λ2
λ3 – λ1
A2 – A3 +([A1 – A3] * )( )
Flow-Through Accessory
• Optical glass or quartz flowcell
• Great for speeding up sample throughput
• Selectable tubing and pumping times accommodate sample volumes from 2 – 15 mL
• Option to return sample to vessel
Filter Holders
• Used to measure transmittance and cut-off wavelengths in glass and filters
• Both spring-loaded and adjustable holders are available
Test Tube Holder
• Ideal for COD tubes and glassware fromreagent kits
• Holds test tubes up to 25 mm diameter and up to 102 mm tall
Funnel Flowcell
• Fast sample throughput
• Used with external vacuum pump orwater aspiration system
• Used on Single Cell Platform
Longpath Cell Holders
• Used to increase sensitivity of readings
• Hold rectangular or cylindrical cells
• Hold up to three 50 mm pathlength cells in 6-Position Cell Holder or one 100 mm pathlengthcell in Single Cell Platform
Faster Measurements Using
Sample Holder of Your Choice
Before the software can work its magic, you need aninstrument versatile enough to accommodate a widerange of sample holders. The Spectronic GENESYS 10spectrophotometer gives you an impressive range ofchoices for fast, efficient sample measurements.
Typical printout showing wavelength
accuracy, photometric accuracy, noise and stray light measurements
Versatile Laboratory
Applications
• Water and wastewater• Food and beverages• Inorganics• Quality control of chemicals• Life science
Visible and UV-Visible Spectrophotometers
Spectronic GENESYS 10
Analyze • Detect • Measure • Control™
Industrial QC
Chemical
Food & Beverage
Environmental
Academic
Scientists, researchers, and techniciansworldwide have long depended on ThermoElectron Corporation to provide Visible andUV-Visible spectrophotometers to suit theirspecific applications and budgets. TheSpectronic GENESYS 10 spectrophotometerseries is the standard that lab managers cancount on to deliver accurate, reliable resultsfor top productivity in the lab.
Meet the New Laboratory Standard
BR50857_E 12/04M
Thermo Electron Scientific Instruments Corporation, Madison, WI USA is ISO Certified.
©2004 Thermo Electron Corporation. All rights reserved.All trademarks are the property of Thermo ElectronCorporation and its subsidiaries.
Specifications, terms and pricing are subject to change.Not all products are available in all countries. Pleaseconsult your local sales representative for details.
In addition to these offices, ThermoElectron Corporation maintains a network of representative organizationsthroughout the world.
Australia+61 2 9898 1244 • [email protected]
Austria+43 1 333 50340 • [email protected]
Belgium+32 2 482 30 30 • [email protected]
Canada+1 800 532 4752 • [email protected]
China+86 10 5850 3588 • [email protected]
France+33 1 60 92 48 00 • [email protected]
Germany+49 6103 4080 • [email protected]
Italy+39 02 950 591 • [email protected]
Japan+81 45 453 9100 • [email protected]
Netherlands+31 76 587 98 88 • [email protected]
Nordic+46 8 556 468 00 • [email protected]
South Africa+27 11 570 1840 • [email protected]
Spain+34 91 657 4930 • [email protected]
Switzerland+41 61 48784 00 • [email protected]
UK+44 1442 233555 • [email protected]
USA+1 800 532 4752 • [email protected]
www.thermo.com
Spectronic Spectronic SpectronicGENESYS 10 Vis GENESYS 10 UV-Vis GENESYS 10 UV-Vis ScanningPart Number 335900 Series Part Number 335902 Series Part Number 335906 Series
Spectral Bandwidth 5 nm
Optical Design Single-beam, grating-based, Split-beam, grating-based, dual detectorssingle detector
Lamp Source Tungsten-Halogen (1000 hrs.) Xenon (5 years)(typical service life)
Wavelength:Range 325 – 1100 nm 190 – 1100 nmAccuracy ± 1.0 nm ± 1.0 nmRepeatability ± 0.5 nm ± 0.5 nm
Display 320 x 240 pixel backlit LCD, 3.8" x 2.8"
Photometric:Range - 0.1 – 3.0 A; 0.3 – 125%T; ± 9999 CReadout Absorbance, Transmittance, ConcentrationAccuracy 0.5% or 0.005 A, whichever is greater, up to 2 A
Noise < 1 mA at 0 A; < 2 mA at 2 A, peak-to-peak at 340 nm
Drift < 2 mA/hour (after warmup) < 1 mA/hour
Stray Light < 0.1%T at 340 and 400 nm < 0.1%T at 220, 340, and 400 nm
Data Interface Bi-directional RS232C Bi-directional RS232C (text only); parallel(text only) output in HP PCL format (text and graphics)
Cell Holder 1-position or automatic 6-position Cell Holder
Keyboard Membrane keypad
Software
Test Storage Up to 80 sets of test parameters
Languages Software, printouts and operator’s manual: English, French, German, Spanish, Italian (user selectable)
Internal Printer 40-column internal graphics(optional)
Analog Output 0 – 1 V for - 0.3 to 2.5 A Not available(optional)
Power Requirements Selected automatically; 100 – 240 Volts
Dimensions 300 W x 400 D x 250 H mm (11.8" x 15.7" x 9.8")
Weight 8.6 kg (19 lb.)
Warranty 1 year
Absorbance/Transmittance/ConcentrationStandard Curve Absorbance RatioAbsorbance DifferenceKineticsSurvey Scan (100 nm increments)3-Point NetMultiwavelengthPerformance Validation
Same as Spectronic GENESYS 10 UV-Visbut with full-range scanningScanning Speed: 200 – 1000 nm/minSampling Interval: 1, 2, 3, 5 nmCell CorrectionSmartStart Program
m o l e c u l a r s p e c t r o s c o p y
![QM-IDI : LIMS / SAP Enterprise Integration Session Code: [3906] Neil Millar Thermo Electron Corporation Neil.millar@thermo.com](https://img.pdfslide.us/doc/110x75/56649d265503460f949fd157/qm-idi-lims-sap-enterprise-integration-session-code-3906-neil-millar.jpg)
